ライフサイエンスコーパス: TFIIIA

1 TFIIIA also functions in transport and storage of 5 S RN

2 TFIIIA alters CPD formation primarily in the transcribed

3 TFIIIA and 42sp43 were also very active transcriptionall

4 TFIIIA can bind to the somatic 5S rRNA gene assembled in

5 TFIIIA from S.cerevisiae or vertebrates contains a total

6 TFIIIA is essential for RNA polymerase III-based transcr

7 TFIIIA is required to activate RNA polymerase III transc

8 TFIIIA regulates 5S rRNA synthesis and is the prototype

9 TFIIIA zinc fingers 1-3 are connected by highly conserve

10 rystal structures of a nucleosome core and a TFIIIA-DNA cocomplex yields a precise picture of the ter

11 ergent N- and C-terminal regions harboring a TFIIIA-type zinc finger and helix-turn-helix DNA-binding

12 nding inhibition, whereas preincubation of a TFIIIA/5S RNA complex with lead did not result in DNA-bi

13 Additionally, TFIIIA binds the internal promoter of the 5S RNA gene an

14 H2A-H2B dimer or the H3-H4 tetramer affects TFIIIA binding to its cognate DNA site within the 5S nuc

15 ficantly relieves this inhibition and allows TFIIIA to exhibit high-affinity binding to nucleosomal D

16 Here it is reported that amphibian TFIIIA proteins contain a sequence element with homology

17 er C-terminal tails, mammalian and amphibian TFIIIAs share a conserved transcription activation domai

18 nserved residues found in fish and amphibian TFIIIAs.

19 anying conserved residues found in amphibian TFIIIAs but not found in yeast or human TFIIIAs.

20 By analyzing TFIIIA mutants with disruptions of individual zinc finge

21 f use in the design of 5 S RNA molecules and TFIIIA peptides for structural studies of the interactio

22 robably involved in pre-rRNA processing, and TFIIIA.

23 nto the structure and function of TFIIIA and TFIIIA-type zinc finger proteins.

24 factors, such as Sp1, AP1, AP2, AP3, Y1, and TFIIIA, were identified in the 5'-region of the gene and

25 muno-precipitation of the complex using anti-TFIIIA-intP antibody.

26 e of three boxes (A, IE, or C) designated as TFIIIA contact sites in the ICR, while one site is betwe

27 ed the identity of this divergent protein as TFIIIA by showing that it binds specifically and with hi

28 se correspond to the A and C boxes that bind TFIIIA, found in the genes from other genera.

29 ween +50 and +60, which functions in binding TFIIIA.

30 Because 5S RNA binds TFIIIA zinc fingers, this result is consistent with an i

31 ame extent as X. laevis and Xenopus borealis TFIIIAs.

32 UV photofootprinting of the 50 bp TFIIIA binding region of 5S rDNA (or ICR) shows that TFI

33 Zinc-fingers mostly interact with DNA, but TFIIIA binds not only specifically to the promoter DNA,

34 ducts in 5S rDNA can be markedly affected by TFIIIA binding, and complex formation is inhibited by UV

35 nontemplate strand is much less affected by TFIIIA binding, where only one CPD site is inhibited in

36 We propose that RNA binding by TFIIIA shows similarity to DNA binding in the use of the

37 The energetics of DNA binding by TFIIIA thus emerges as a compromise between individual f

38 le another CPD site is strongly enhanced, by TFIIIA binding.

39 ancy of a subset of the oocyte-type genes by TFIIIA that become positioned at the nuclear periphery s

40 NER complex could be sterically hindered by TFIIIA.

41 n of DNase I protection patterns produced by TFIIIA from multiple species suggests a novel mode of DN

42 d at several sites in the template strand by TFIIIA binding.

43 The C2H2 TFIIIA/Kruppel class of zinc finger proteins are an impo

44 ave purified and characterized A.castellanii TFIIIA (AcTFIIIA) as a step toward obtaining a clearer u

45 Catfish TFIIIA lacks the conserved transcription activation doma

46 Catfish TFIIIA was able to bind the catfish and Xenopus 5S RNA g

47 The N-terminal region of catfish TFIIIA contains the oocyte-specific initiating Met amino

48 The zinc fingers of Saccharomyces cerevisiae TFIIIA are interrupted between fingers eight and nine by

49 at these three sites may partially displace TFIIIA, thereby enabling rapid repair.

50 tion of the TFIIIA/5S rDNA complex displaces TFIIIA at doses of 0.8-2 CPDs/fragment, indicating the c

51 N-Terminal zinc fingers from either TFIIIA or p43 bind to multiple specific sites on 5S RNA

52 nuclear extract immunodepleted of endogenous TFIIIA.

53 Gene-specific factor TFIIIA interacts with DNA and acts as an adaptor for TFI

54 gnate binding sites for transcription factor TFIIIA are significantly more accessible when the rDNA i

55 specific region of the transcription factor TFIIIA binding site interferes with 5S RNA gene expressi

56 ine-zinc finger Xenopus transcription factor TFIIIA the central group of fingers, fingers 4 to 7, hav

57 te for the 5 S-specific transcription factor TFIIIA was systematically varied and the relative bindin

58 chemical studies on the transcription factor TFIIIA, which regulates the 5S ribosomal RNA genes of Xe

59 ding of the primary 5S transcription factor, TFIIIA.

60 rRNA promoter requires transcription factors TFIIIA, TFIIIC, and TFIIIB.

61 ined the DNA binding activity of nine finger TFIIIA and shorter recombinant analogs in the presence o

62 central regions of the complete nine-finger TFIIIA-5S RNA complex.

63 mino acids required for zinc finger folding, TFIIIAs from different species are remarkably divergent,

64 varied and the relative binding affinity for TFIIIA determined.

65 III display minor decreases in affinity for TFIIIA.

66 These results reveal a novel function for TFIIIA as a negative regulator that recruits histone mod

67 pecific arsenic ions were not inhibitory for TFIIIA binding.

68 of the human homolog (termed TFIIIA-intP for TFIIIA-interacting protein) was determined from expresse

69 In order to identify protein partners for TFIIIA, yeast two-hybrid screens were performed using th

70 Preincubation of free TFIIIA with lead resulted in DNA-binding inhibition, whe

71 d RNA removal, whereas preincubation of free TFIIIA with the metal ions resulted in inhibition of sub

72 is composed of (His)2(Cys)2, such as, e.g., TFIIIA.

73 tions involving 5 S rRNA, the 5 S rRNA gene, TFIIIA, and L5 mediates both feedback inhibition of 5 S

74 core histones within the 5S DNA-core histone-TFIIIA triple complex is not changed upon TFIIIA associa

75 ults in very stable, kinetically homogeneous TFIIIA-DNA complexes.

76 er to investigate the molecular basis of how TFIIIA binds to the nucleosome and to ascertain if bindi

77 These results show how TFIIIA can recognize several separated DNA sequences by

78 However, TFIIIA binds with high affinity to 5S nucleosomes lackin

79 However, TFIIIA is not completely displaced during NER, at least

80 The N-terminal regions of rodent and human TFIIIA do not contain the oocyte-specific initiating Met

81 onservation in catfish, amphibian, and human TFIIIA zinc fingers allows deduction of possible finger

82 C-terminal tail found in amphibian and human TFIIIA.

83 mbinant nine-finger form of a putative human TFIIIA clone.

84 In vitro interaction of recombinant human TFIIIA-intP with recombinant Xenopus TFIIIA was demonstr

85 und to rat and mouse TFIIIA but not to human TFIIIA in Western blots.

86 Rodent and human TFIIIAs share about 87% aa sequence identity in their zi

87 bian TFIIIAs but not found in yeast or human TFIIIAs.

88 inding of individual zinc fingers of the ICR-TFIIIA complex.

89 fate) in the zinc finger contacts of the ICR-TFIIIA complex.

90 Transcription factor IIIA (TFIIIA) activates 5S ribosomal RNA gene transcription in

91 finger domains of transcription factor IIIA (TFIIIA) and a 15-base-pair DNA duplex was investigated b

92 nc finger protein transcription factor IIIA (TFIIIA) and binding of its individual zinc fingers was s

93 million fold more transcription factor IIIA (TFIIIA) and its corresponding mRNA than in a somatic cel

94 Transcription factor IIIA (TFIIIA) and p43 zinc finger protein form distinct comple

95 Transcription factor IIIA (TFIIIA) binds to 5S rRNA transcripts and this interactio

96 Xenopus transcription factor IIIA (TFIIIA) binds to over 50 base pairs in the internal cont

97 The gene-specific transcription factor IIIA (TFIIIA) binds to the internal promoter element of the 5

98 of Xenopus laevis transcription factor IIIA (TFIIIA) bound to its cognate DNA duplex was determined b

99 of Xenopus laevis transcription factor IIIA (TFIIIA) bound with 31 bp of the 5S rRNA gene promoter ha

100 Zinc fingers in transcription factor IIIA (TFIIIA) contribute differentially to RNA and DNA binding

101 ne finger protein transcription factor IIIA (TFIIIA) from Xenopus oocytes binds a 43 base pair region

102 RNA gene-specific transcription factor IIIA (TFIIIA) interacts with the small ubiquitin-like modifier

103 Transcription factor IIIA (TFIIIA) is a Cys2His2 zinc finger protein that regulates

104 Xenopus transcription factor IIIA (TFIIIA) is phosphorylated on serine-16 by CK2.

105 Transcription factor IIIA (TFIIIA) is specifically required for transcription of 5S

106 ts the binding of transcription factor IIIA (TFIIIA) to its cognate DNA sequence within the internal

107 Binding of transcription factor IIIA (TFIIIA) to site-specific mutants of Xenopus oocyte 5 S r

108 5S gene-specific transcription factor IIIA (TFIIIA) to the 5S internal promoter.

109 cDNA for rat transcription factor IIIA (TFIIIA) was cloned by degenerate PCR and rapid amplifica

110 Transcription factor IIIA (TFIIIA), a cysteine-rich regulatory protein, is the prot

111 contact site for transcription factor IIIA (TFIIIA), accounting for the mutually exclusive binding o

112 of overexpressing transcription factor IIIA (TFIIIA), the 5 S rRNA gene-specific transcription factor

113 nscriptionally by transcription factor IIIA (TFIIIA), which upon transcription, binds 5S rRNA, formin

114 using the Xenopus transcription factor IIIA (TFIIIA)-5S rDNA complex and Xenopus oocyte nuclear extra

115 nc finger protein transcription factor IIIA (TFIIIA).

116 erties of Xenopus transcription factor IIIA (TFIIIA).

117 o which tail acetylation or removal improves TFIIIA binding cannot be simply explained by a commensur

118 for the wild-type leucine at position 148 in TFIIIA results in much larger compensating changes in th

119 the same putative alpha-helix amino acids in TFIIIA zinc fingers are essential for both RNA and DNA b

120 The 5S rRNA-derived exon in TFIIIA gene exists in all representative land plant spec

121 omains does not lead to further increases in TFIIIA binding.

122 itution at these four positions were made in TFIIIA RNA binding zinc fingers, tz4-7 and DNA binding z

123 e tail domains directly negatively influence TFIIIA binding to the nucleosome in a manner that requir

124 hether in an isolated group or in the intact TFIIIA molecule.

125 strong support for the application to intact TFIIIA of recent structural models of the N-terminal zin

126 nct from the smaller footprint of the 51 kDa TFIIIA from Saccharomyces cerevisiae.

127 Although all known TFIIIA homologs harbor nine zinc fingers that mediate DN

128 to a promoter cis element in Xenopus laevis TFIIIA gene (B3).

129 and 4 to 7, respectively, and of full-length TFIIIA.

130 ese clones code for the respective mammalian TFIIIAs.

131 p53 tumor suppressor bound to rat and mouse TFIIIA but not to human TFIIIA in Western blots.

132 A putative mouse TFIIIA clone was identified in an expressed sequence tag

133 cleotides protected by Xenopus laevis native TFIIIA on the Xenopus 5S RNA gene internal control regio

134 Seven of the nine TFIIIA zinc fingers participate in major groove DNA cont

135 of the Xenopus 5S ribosomal RNA gene but not TFIIIA-independent transcription of the human adenovirus

136 to the DNA complexes of zinc fingers 1-3 of TFIIIA (zf1-3) and the four zinc fingers of the Wilms' t

137 and Al3+ ions also inhibited the ability of TFIIIA to bind complementary single-stranded DNA and pro

138 and/or H2B, directly modulate the ability of TFIIIA to bind nucleosomal DNA.

139 effect on the DNA or RNA binding activity of TFIIIA.

140 e-DNA interactions attenuate the affinity of TFIIIA for its cognate DNA element by a factor of 50-100

141 he neurula stage, as is a limiting amount of TFIIIA.

142 ed 5S rRNA in quantitative autoregulation of TFIIIA homeostasis.

143 Mutations in helix V affect binding of TFIIIA to 5 S rRNA and to the gene similarly and provide

144 Binding of TFIIIA to the substrates was assayed by measurement of d

145 cule known to be important in the binding of TFIIIA.

146 cription efficiency parallels the binding of TFIIIA.

147 a direct role in restricting the binding of TFIIIA.

148 e the identification and characterization of TFIIIA from Schizosaccharomyces pombe.

149 identical to that of the ternary complex of TFIIIA and TFIIIC bound to a somatic 5S rRNA gene.

150 M) to visualize the S. cerevisiae complex of TFIIIA and TFIIIC bound to the promoter.

151 tor element of tRNA genes and the complex of TFIIIA with a 5S rRNA gene.

152 -terminal transcription activation domain of TFIIIA.

153 ing the three amino-terminal zinc fingers of TFIIIA (zf1-3) co-occupy the TFIIIA binding site, in agr

154 ion indicates that the N-terminal fingers of TFIIIA are affected by the metal ions.

155 ur data reveal that the first six fingers of TFIIIA bind and displace approximately 20 bp of histone-

156 residues in more C-terminal zinc fingers of TFIIIA for which high-resolution structural information

157 ltaneous binding by all nine zinc fingers of TFIIIA may involve a substantial energetic cost.

158 ing energy among the various zinc fingers of TFIIIA remains poorly understood.

159 minimally involves the N-terminal fingers of TFIIIA.

160 ltaneous binding by all nine zinc fingers of TFIIIA.

161 midone (6-4) dimers, reduced the fraction of TFIIIA bound by approximately 70%.

162 insights into the structure and function of TFIIIA and TFIIIA-type zinc finger proteins.

163 used to probe the structure and function of TFIIIA.

164 central fingers within helices IV and II of TFIIIA.

165 e I footprinting show a strong inhibition of TFIIIA binding to UV-damaged 5S rDNA.

166 The inhibition of TFIIIA function in vitro by xenobiotic metals offers new

167 contributes positively to the initiation of TFIIIA gene transcription in Xenopus oocytes.

168 polyamides block the specific interaction of TFIIIA or zf1-4 with the 5 S RNA gene, supporting a mode

169 ed by DNase I protection, the interaction of TFIIIA with the 50-bp internal control region of the 5S

170 The specific interaction of TFIIIA with the internal control region (ICR) of the 5S

171 ICR does not significantly affect the Kd of TFIIIA.

172 t site) in the ICR markedly enhanced koff of TFIIIA from the complex.

173 These high levels of TFIIIA gene expression are achieved primarily by transcr

174 udies with COOH-terminal deletion mutants of TFIIIA and 5S nucleosomes reconstituted with native and

175 tion to generate and screen large numbers of TFIIIA mutants for those with altered 5 S rRNA gene-bind

176 Phosphorylation of TFIIIA by CK2 may allow the factor to continue to act as

177 Preincubation of TFIIIA bound to 5S RNA with either Cd2+ or Al3+ resulted

178 enopus TFIIIA that increase the stability of TFIIIA-5 S rRNA gene complexes.

179 This nucleosome may act positively on TFIIIA transcription in oocytes by placing transcription

180 Catfish oocyte TFIIIA was identified by its association with 5S rRNA in

181 cloning and functional divergence of oocyte TFIIIA from the channel catfish.

182 59 kDa, significantly larger than all other TFIIIA homologs isolated to date.

183 tiple independent Arabidopsis overexpressing TFIIIA transgenic lines under osmotic and salt stress, s

184 In contrast, the S.pombe TFIIIA sequence includes ten potential zinc finger motif

185 Recombinant S.pombe TFIIIA was also shown to support specific transcription

186 for the transcriptional function of S.pombe TFIIIA, but neither is required for wild-type 5S rRNA ge

187 and virus-associated RNA genes) or promoter-TFIIIA complexes (for the 5S RNA gene) and subsequent re

188 Interaction of rat TFIIIA with rat TFIIIA-intP was indicated by co-chromato

189 e tag database by sequence similarity to rat TFIIIA.

190 Interaction of rat TFIIIA with rat TFIIIA-intP was indicated by co-chromatography of the tw

191 In a HeLa cell nuclear extract, recombinant TFIIIA-intP was able to stimulate TFIIIA-dependent trans

192 the gene of its own transcription regulator, TFIIIA (transcription factor for polymerase III A).

193 transcription factor-1 (MTF-1), contains six TFIIIA-type Cys(2)-His(2) motifs, each of which was proj

194 ns, which reflects unique properties of some TFIIIA homologs.

195 ecombinant TFIIIA-intP was able to stimulate TFIIIA-dependent transcription of the Xenopus 5S ribosom

196 moval of the histone tail domains stimulates TFIIIA binding to the 5S nucleosome greater than 100-fol

197 on activation domain dramatically stimulates TFIIIA binding to the native nucleosome, while further C

198 imary structure of the human homolog (termed TFIIIA-intP for TFIIIA-interacting protein) was determin

199 egree of homology occurs in pol III, TFIIIB, TFIIIA and the three initiation-related subunits of TFII

200 c histone-to-DNA cross-linking, we show that TFIIIA binding neither induces nor requires nucleosome m

201 inding region of 5S rDNA (or ICR) shows that TFIIIA binding modulates photoproduct formation primaril

202 ent at -30, we show that from -425 to +7 the TFIIIA gene contains only two positive cis elements cent

203 Because the TFIIIA aa sequence is highly diverged, elucidating speci

204 Because 5S rRNA also binds the TFIIIA zinc finger domains, these results indicate that

205 lly characterized zinc finger complexes, the TFIIIA complex exhibits several novel features.

206 of histone-DNA interactions, we examined the TFIIIA-nucleosome complex by hydroxyl radical footprinti

207 nting and mutagenesis data available for the TFIIIA-DNA complex.

208 2)His(2)-type zinc finger, distinct from the TFIIIA-type, is extended at its C terminus by two additi

209 This arrangement of the cis elements in the TFIIIA gene is striking because these two elements are p

210 thermodynamically unfavorable strain in the TFIIIA.5 S rRNA gene complex may be derived from bending

211 ngers at opposite ends of the protein in the TFIIIA.5 S rRNA gene complex.

212 Aluminum ions were also found to inhibit the TFIIIA-5S RNA gene interaction, albeit at higher concent

213 zinc fingers of TFIIIA (zf1-3) co-occupy the TFIIIA binding site, in agreement with the known locatio

214 The proteins that bind E1 and E3 of the TFIIIA gene have been identified as Xenopus USF (Xl-USF)

215 ve cis-elements in the control region of the TFIIIA gene located at positions -269 to -264 (E1), -235

216 Xl-USF binds to element 1 of the TFIIIA gene which is immediately adjacent to element 2.

217 ted to assess the role of this region of the TFIIIA molecule in more detail than hitherto.

218 terminus and contains 16 zinc fingers of the TFIIIA subclass, in comparison to human RREB-1 which was

219 Members of the TFIIIA superfamily contain Cys2His2 zinc finger domains

220 otein containing 29 C2H2 zinc fingers of the TFIIIA type.

221 e existing NMR and crystal structures of the TFIIIA-DNA complex.

222 These findings support the role of the TFIIIA-type Zn fingers in both protein-protein interacti

223 Furthermore, irradiation of the TFIIIA/5S rDNA complex displaces TFIIIA at doses of 0.8-

224 Efficient repair occurs just outside the TFIIIA-binding site (within 10 bp), and in the absence o

225 We further show that the TFIIIA NES can functionally replace the NES of BR1 in bo

226 It is further demonstrated that this TFIIIA sequence element functions as a protein nuclear e

227 rs in RNA and DNA binding we exchanged three TFIIIA linker amino acids with the equivalent amino acid

228 sed to probe the binding to 5 S RNA of three TFIIIA peptides Tf(1-6), Tf(4-6) and Tf(4-7), consisting

229 Thus, TFIIIA binding occurs by displacement of H2A-H2B-DNA con

230 se results indicate that the 5S RNA bound to TFIIIA protects the protein from metal inhibition and im

231 s at levels comparable to those of wild-type TFIIIA; however, there is no transcription of the oocyte

232 ne-TFIIIA triple complex is not changed upon TFIIIA association.

233 to those produced by the smaller vertebrate TFIIIA homologs, but distinct from the smaller footprint

234 All known vertebrate TFIIIAs have a similar organization: nine zinc fingers,

235 ence indeed occurs between zinc fingers when TFIIIA binds to the 5 S rRNA gene and that the greatest

236 t most sites in both strands of the ICR when TFIIIA is bound.

237 anscribed strand)] are repaired rapidly when TFIIIA is bound, while CPDs within approximately 5 bases

238 obtain insights into the mechanism by which TFIIIA recognizes 5 S RNA, we determined the solution st

239 RNAPIII transcription system, as did Xenopus TFIIIA.

240 We have identified four mutations in Xenopus TFIIIA that increase the stability of TFIIIA-5 S rRNA ge

241 human immunodeficiency virus and in Xenopus TFIIIA.

242 ormed using the C-terminal region of Xenopus TFIIIA as an attractor and a rat cDNA library as a sourc

243 with the DNA-binding specificity of Xenopus TFIIIA is expressed in yeast cells, where it specificall

244 as the basis for a genetic assay of Xenopus TFIIIA's DNA-binding function in yeast, an assay that we

245 Exemplified by the prototypic Xenopus TFIIIA protein, and more recently by mammalian p53, this

246 t human TFIIIA-intP with recombinant Xenopus TFIIIA was demonstrated by immuno-precipitation of the c

247 The selective expression of the Xenopus TFIIIA gene in immature oocytes is principally regulated

248 The Xenopus TFIIIA gene is transcribed very efficiently in oocytes.

249 uence identity was observed with the Xenopus TFIIIA zinc finger region.

250 atively to allow the affinity of the Xenopus TFIIIA-5 S rRNA gene interaction to be deduced from meas

251 rotein in yeast that interacted with Xenopus TFIIIA but not with yeast TFIIIA.

252 sequence identity was observed with Xenopus TFIIIA.

253 y peptide sequence similarities with Xenopus TFIIIA.

254 acted with Xenopus TFIIIA but not with yeast TFIIIA.