ライフサイエンスコーパス: TIRF

1 TIRF images were constructed from several layers within

2 TIRF imaging indicates that the granules can be triggere

3 TIRF imaging of this cortical pool demonstrates more Pom

4 TIRF microscopic observations of functional ElmoA-GFP re

5 TIRF microscopy and biophysical modeling of fluorescence

6 TIRF microscopy and cryo-correlative light microscopy an

7 TIRF microscopy can be used in conjunction with CFP/YFP

8 TIRF microscopy experiments revealed that increasing the

9 TIRF microscopy localized the entering virus-like partic

10 TIRF provides a superior signal-to-noise ratio, but we a

11 TIRF was used to monitor biospecific interactions, while

12 TIRF-PBM provides a novel and extendible platform for mu

13 TIRF/FRET experiments revealed cotransfection of wild-ty

14 This live-cell 2D TIRF-SIM-TFM methodology offers a combination of spatio-

15 emonstrate, using quantitative live-cell 4D, TIRF, and FRET imaging, that endocytosis and trafficking

16 hly sensitive fluorescence immunoassay for a TIRF (total internal reflection)-based point-of-care tes

17 By attaching a rolling-circle substrate to a TIRF microscope-mounted flow chamber, we are able to mon

18 sformed to match the results obtained with a TIRF-based structured illumination microscope.

19 monstrate in vitro using assembly assays and TIRF microscopy, and in primary neurons using live-cell

20 Using in vitro bulk and TIRF microscopy assays, we find that DAAM2 variants alte

21 umin was injected in the channel chamber and TIRF was used to determine the time to reach the assay s

22 resulting dissociation of Arp2/3 complex and TIRF microscopy to visualize filament severing and the l

23 h community by bridging between confocal and TIRF detection geometries in a simple and efficient way.

24 ondria, as witnessed by dynamic confocal and TIRF microscopy.

25 , as judged by epifluorescent, confocal, and TIRF microscopy, but fluoresces brightly within the Ser-

26 Similarly, electrophysiological data and TIRF microscopy show that NEDD4 unrestrained mutant cons

27 s-based prescribing assessments; (3) FDA and TIRF sponsor communications; (4) modifications to the RE

28 Using FLIM and TIRF imaging, we find that changes in MCAK conformation

29 e effective viscosity of the bulk lipid, and TIRF microscopy indicates that it clusters in segregated

30 aneous membrane capacitance measurements and TIRF imaging.

31 ro reconstitution with purified proteins and TIRF microscopy to investigate the activity of human SSN

32 it achieves a similar optical sectioning as TIRF does.

33 lecule microscopy and microfluidics-assisted TIRF imaging, we demonstrate that twinfilin transiently

34 Here, we use in vitro microfluidics-assisted TIRF microscopy to show that the C terminus of CAP promo

35 Using microfluidics-assisted TIRF microscopy, we find that formin, CP and twinfilin c

36 Here, using microfluidics-assisted TIRF microscopy, we show that mouse twinfilin, a member

37 Using microfluidics-assisted TIRF, we show that Cyclase-associated protein (CAP) and

38 malian cells with any commercially available TIRF microscope.

39 Commercially available TIRF systems are either objective based that employ expe

40 l, inexpensive, LED powered, waveguide based TIRF system that could be used as an add-on module to an

41 lection fluorescence, fiber-optic biosensor (TIRF-FOB) for protein detection, which integrates a lipo

42 ingle-vesicle fusion events in PC12 cells by TIRF micro-scopy.

43 distinct fluorescent signals as detected by TIRF microscopy.

44 ion factors TBP, TFIIA and IIB determined by TIRF-PBM are similar to those determined by traditional

45 the time evolution of individual fibrils by TIRF microscopy.

46 Single-vesicle optical (by TIRF microscopy) and biophysical measurements of ATP rel

47 on behavior of these particles was probed by TIRF microscopy on bleb-derived supported membranes.

48 These results are further supported by TIRF data for LIG1 binding to DNA with a single nick sit

49 When tracked by TIRF and spinning-disk microscopy, cells expressing phos

50 ) trafficking pathways as shown by live cell TIRF and structured illumination microscopy (SIM).

51 vents at the plasma membrane using live cell TIRF microscopy.

52 amin2-EGFP instead of dynamin2 and live-cell TIRF imaging with single-molecule EGFP sensitivity and h

53 We used live-cell TIRF imaging with single-molecule EGFP sensitivity and h

54 Live-cell TIRF microscopy revealed that SHCA clustering at the cel

55 M and dSTORM super-resolution, and live-cell TIRF microscopy to characterize the structural organizat

56 Using live-cell TIRF microscopy, we demonstrated that ATG9A-positive ves

57 nd tracked the motor pairs using two-channel TIRF microscopy.

58 Using four-color TIRF, we show that IQGAP1's displacement activity extend

59 Utilizing multi-color TIRF microscopy of in vitro reconstituted F-actin networ

60 However, two-color TIRF microscopy using fluorescent proteins fused to clat

61 Using a microfluidics-assisted multi-colour TIRF microscopy assay with close-to-nm and sub-second pr

62 By combining TIRF microscopy and a stochastic model of exocytosis, we

63 elds, and we provide a roadmap for comparing TIRF data across images, experiments, and laboratories.

64 and allows switching between epifluorescence/TIRF/bright field modes without adjustments or objective

65 measure of ligand-receptor binding, an FCS & TIRF receptor dimerization assay was developed to measur

66 Overall, the results suggest that the FCS & TIRF receptor dimerization assay can assess FGFR dimeriz

67 ese nanoparticles using ITC, DLS, FRET, FCS, TIRF, and TEM.

68 on cryoEM reconstruction and single filament TIRF microscopy we identify two dynamic and structural s

69 ing total internal reflectance fluorescence (TIRF) microscopy, we found that beta-catenin is required

70 of total internal reflectance fluorescence (TIRF) spectroscopy, swellable hydrogel double-stranded D

71 sing total internal reflection fluorescence (TIRF) and confocal microscopy, we studied the mechanisms

72 h as total internal reflection fluorescence (TIRF) and Forster resonance energy transfer (FRET) has p

73 ular total internal reflection fluorescence (TIRF) configuration.

74 Total internal reflection fluorescence (TIRF) confines fluorescence excitation by an evanescent

75 of a total internal reflection fluorescence (TIRF) flow cell.

76 in a total internal reflection fluorescence (TIRF) format.

77 T in total-internal reflection fluorescence (TIRF) Forster resonance energy transfer (TIRF-FRET) micr

78 cing total internal reflection fluorescence (TIRF) images that are evenly lit.

79 d by total internal reflection fluorescence (TIRF) imaging and iterative particle image velocimetry (

80 Total-internal reflection fluorescence (TIRF) imaging is capable of determining membrane-binding

81 and total internal reflection fluorescence (TIRF) imaging to visualize MV scanning in the context of

82 ion, total internal reflection fluorescence (TIRF) imaging was used to visualize the migration of flu

83 with total internal reflection fluorescence (TIRF) imaging.

84 Total-internal reflection fluorescence (TIRF) microscope is a unique technique for selective exc

85 ngle total internal reflection fluorescence (TIRF) microscope.

86 with total internal reflection fluorescence (TIRF) microscopy allowed us to image GFP-tagged SMSr pro

87 ning total internal reflection fluorescence (TIRF) microscopy and a series of complementary Fluoresce

88 cule total internal reflection fluorescence (TIRF) microscopy and allows the probing of single macrom

89 sing total internal reflection fluorescence (TIRF) microscopy and BS(3) cross-linking, we determined

90 Total internal reflection fluorescence (TIRF) microscopy and its variants are key technologies f

91 n of total internal reflection fluorescence (TIRF) microscopy and molecular dynamics (MD) simulations

92 sing total internal reflection fluorescence (TIRF) microscopy and observed intramolecular condensatio

93 sing total internal reflection fluorescence (TIRF) microscopy and patch-clamp recording from single J

94 ined total internal reflection fluorescence (TIRF) microscopy and patch-clamp recording to localize S

95 cule total internal reflection fluorescence (TIRF) microscopy constitutes an umbrella of powerful too

96 itro total internal reflection fluorescence (TIRF) microscopy demonstrated that Tpm1 strongly enhance

97 with total internal reflection fluorescence (TIRF) microscopy demonstrates that the sensor can be use

98 that total internal reflection fluorescence (TIRF) microscopy images of subcellular structures within

99 ore, total internal reflection fluorescence (TIRF) microscopy imaging of single actin filaments confi

100 u by total-internal-reflection fluorescence (TIRF) microscopy imaging.

101 and total internal reflection fluorescence (TIRF) microscopy in combination with fluorescence recove

102 cule total internal reflection fluorescence (TIRF) microscopy in solid-supported lipid bilayers and s

103 and total internal reflection fluorescence (TIRF) microscopy in vitro, and the mechanism mimics the

104 Total internal reflection fluorescence (TIRF) microscopy is a rapidly expanding optical techniqu

105 apse total internal reflection fluorescence (TIRF) microscopy is used to directly measure the kinetic

106 tion total internal reflection fluorescence (TIRF) microscopy of live cells, we followed the movement

107 and total internal reflection fluorescence (TIRF) microscopy of live tumor cells, revealing that flu

108 and total internal reflection fluorescence (TIRF) microscopy of living mammalian cells and correlati

109 and total internal reflection fluorescence (TIRF) microscopy revealed that HSV-1 was released at spe

110 Total internal reflection fluorescence (TIRF) microscopy reveals highly mobile structures contai

111 olor total internal reflection fluorescence (TIRF) microscopy reveals that a low number of INF2 molec

112 apse total internal reflection fluorescence (TIRF) microscopy showed that signaling via the T cell an

113 Total internal reflection fluorescence (TIRF) microscopy suppresses the background from the cell

114 tive total internal reflection fluorescence (TIRF) microscopy system to directly visualize the moveme

115 sing total internal reflection fluorescence (TIRF) microscopy that glucose as well as the Ca(2+) mobi

116 and total internal reflection fluorescence (TIRF) microscopy to directly measure the frictional forc

117 sing total internal reflection fluorescence (TIRF) microscopy to image Ca(2+) influx in Xenopus laevi

118 and Total Internal Reflection Fluorescence (TIRF) microscopy to measure lateral diffusion coefficien

119 use total internal reflection fluorescence (TIRF) microscopy to probe individual QDs immobilized on

120 use total internal reflection fluorescence (TIRF) microscopy to show that Dip1 causes actin assemble

121 cule total internal reflection fluorescence (TIRF) microscopy to study their interactions.

122 used total internal reflection fluorescence (TIRF) microscopy to track the interactions between micro

123 Total internal reflection fluorescence (TIRF) microscopy was used to quantify the growth of sing

124 lly, total internal reflection fluorescence (TIRF) microscopy was used to track docked vesicles and o

125 ning total internal reflection fluorescence (TIRF) microscopy with fluorescence recovery after photob

126 nce, total internal reflection fluorescence (TIRF) microscopy, and live-cell photoactivation localiza

127 nder total internal reflection fluorescence (TIRF) microscopy, and patch-clamp analysis.

128 nder total internal reflection fluorescence (TIRF) microscopy, in which excitation light only penetra

129 By total internal reflection fluorescence (TIRF) microscopy, Scrib and integrin alpha5 colocalize a

130 olor total internal reflection fluorescence (TIRF) microscopy, single particle tracking and motility

131 sing total internal reflection fluorescence (TIRF) microscopy, the fusion of VAMP8-positive vesicles

132 and total internal reflection fluorescence (TIRF) microscopy, we demonstrate that glucose stimulates

133 cule Total Internal Reflection Fluorescence (TIRF) Microscopy, we determined the mechanism controllin

134 sing total internal reflection fluorescence (TIRF) microscopy, we found that TGFbeta enhanced the ass

135 d by total internal reflection fluorescence (TIRF) microscopy, we observed a positive FRET signal.

136 sing total internal reflection fluorescence (TIRF) microscopy, we studied the mechanisms of surface m

137 with total internal reflection fluorescence (TIRF) microscopy, which enables interfacing coherent dia

138 in a total internal reflection fluorescence (TIRF) microscopy-based real-time nucleation assay.

139 sing total internal reflection fluorescence (TIRF) microscopy.

140 nder total internal reflection fluorescence (TIRF) microscopy.

141 sing total internal reflection fluorescence (TIRF) microscopy.

142 cule total internal reflection fluorescence (TIRF) microscopy.

143 with Total Internal Reflection Fluorescence (TIRF) microscopy.

144 sing total internal reflection fluorescence (TIRF) microscopy.

145 llel total internal reflection fluorescence (TIRF) microscopy.

146 tive total internal reflection fluorescence (TIRF) microscopy.

147 and total internal reflection fluorescence (TIRF) microscopy.

148 with total internal reflection fluorescence (TIRF) microscopy.

149 dard total internal reflection fluorescence (TIRF) microscopy.

150 sing total internal reflection fluorescence (TIRF) spectroscopy.

151 sing total internal reflection fluorescence (TIRF) with fluorescence imaging with 1-nm accuracy (FION

152 the total internal reflection fluorescence (TIRF) zone beneath the plasma membrane.

153 with total internal reflection fluorescence (TIRF), confocal, and EM analyses, we show that the N-ter

154 AP), total internal reflection fluorescence (TIRF), deconvolution, and siRNA knockdown, we propose th

155 eld, total internal reflection fluorescence (TIRF), super-resolution, single-molecule, and laser-scan

156 and total internal reflection fluorescence (TIRF), to investigate the dynamics of LIG1-nick DNA bind

157 and total internal reflection fluorescence (TIRF)-based assay to show that ensembles of kinesin-5, a

158 sing total-internal reflection fluorescence (TIRF).

159 Total-Internal-Reflection-Fluorescence (TIRF) microscopy experiments and data from a laser tweez

160 lamp, Total Internal Reflection Fluorescent (TIRF) microscopy, and fluorescence recovery after photob

161 merit of a high signal/background ratio from TIRF microscopy.

162 at gives rise to nonevanescent components in TIRF.

163 in all experiments could also be verified in TIRF and confocal microscopy.

164 indicated substantial rates of inappropriate TIRF use.

165 r pathological mechanism in AD and introduce TIRF imaging for massively parallel single-channel studi

166 address evidence of high rates of off-label TIRF use, and, although the REMS program had a noncompli

167 ization of TNFR1 was observed via time-lapse TIRF and flow cytometry, and this correlated with increa

168 ing single molecule fluorescence, time-lapse TIRF microscopy and AFM imaging we characterize this phe

169 Using time-lapse TIRF microscopy, we observed and quantified the severing

170 epth) toward the critical angle (the largest TIRF depth) to preferentially photobleach fluorescence f

171 e features of this ultra-sensitive liposomal TIRF-FOB are (i) fluorescence is excited via evanescent

172 ce fluorescence protein-binding microarrays (TIRF-PBM) to evaluate the effects of protein phosphoryla

173 fluorescence and scanning force microscope (TIRF-SFM) to pinpoint fluorescently labeled human homolo

174 internal reflection fluorescence microscopy (TIRF) that a proportion of ARHGAP18 localizes to microtu

175 internal reflection fluorescence microscopy (TIRF).

176 -based total internal reflection microscopy (TIRF) imaging.

177 We envision that our designed mini-TIRF platform will serve as a robust platform for point-

178 total internal reflection fluorescence (mini-TIRF) microscope, we detect the S-RBD and pseudotyped SA

179 Through in silico modeling, TIRF microscopy, and cell-based assays, we determine tha

180 cence microscopy (vaTIRFM) adapted to modern TIRF setup.

181 sslinking mass spectrometry, single molecule TIRF microscopy and biochemical assays identify inter-ho

182 Using a combination of single molecule TIRF microscopy and kinetic analysis of PI(4)P lipid pho

183 tly to Daam1, and multicolor single-molecule TIRF imaging revealed that fascin recruited Daam1 to and

184 Using single-molecule TIRF imaging, we have measured the affiliation of GLP-1

185 ocal microscopy and in vitro single-molecule TIRF imaging, we reveal that Zn2+ inhibits activity of m

186 he low signal/noise ratio in single-molecule TIRF microscopy experiments, it is important to determin

187 Here we use single-molecule TIRF microscopy in living cells to reveal that the enzym

188 We show, using a single-molecule TIRF microscopy technique, that the exchange process is

189 uding azimuthal scanning SAIM and multiangle TIRF.

190 Here, multicolor TIRF microscopy was applied to visualize and quantitativ

191 with purified mammalian proteins, multicolor TIRF-microscopy, and interaction kinetics measurements,

192 structed from several layers within a normal TIRF excitation zone by sequentially imaging and photobl

193 Here, we use a combination of TIRF excitation and supercritical angle fluorescence emi

194 w, making the quantitative interpretation of TIRF data problematic.

195 n of DNA standards to quantify the limits of TIRF-FRET resolution.

196 The optical sectioning of TIRF is based on the excitation confinement of the evane

197 that SAF improves the surface selectivity of TIRF, even at shallow penetration depths.

198 chnique involves the recording of a stack of TIRF images, by gradually increasing the incident angle

199 package amenable to any standard confocal or TIRF microscope.

200 solution using modern widefield, confocal or TIRF microscopes with illumination orders of magnitude l

201 cence structured-illumination microscopy, or TIRF-SIM, to visualize individual myosin II bipolar fila

202 the case of NMIIB-HMM in optical tweezers or TIRF/in vitro motility experiments.

203 ed versions of gammaTuRC, gammaTuNA, and our TIRF assay, the first real-time observation that gammaTu

204 estimator is thus suited for single-particle TIRF microscopy of dense biological samples in which the

205 Single-particle TIRF microscopy shows that wild-type channels in PM have

206 red by monitoring intensity of the polarized TIRF images.

207 ly high levels of knowledge regarding proper TIRF prescribing, yet some survey items as well as claim

208 mechanistic agent based modeling, published TIRF imaging data, and parameter estimation to determine

209 Yet truly quantitative TIRF remains problematic.

210 rs appeared under total internal reflection (TIRF) illumination, and some of them associated with gra

211 pyrene-actin and total internal reflection (TIRF) microscopy elongation assays.

212 ontrast (RIC) and total internal reflection (TIRF) microscopy, respectively.

213 g single-molecule total internal reflection (TIRF) microscopy, we have examined the assembly and disa

214 work paves the way for ultra-high-resolution TIRF-FRET studies on many biomolecules, including DNA pr

215 gle was tuned from the highest (the smallest TIRF depth) toward the critical angle (the largest TIRF

216 azimuthal and polar beam scanning (spinning TIRF), atomic force microscopy, and wavefront analysis o

217 ts with subcellular resolution on a standard TIRF microscope, with a removable Bertrand lens as the o

218 gher than the operational limit for standard TIRF experiments.

219 SW-TIRF is a wide-field superresolution technique with reso

220 e total internal reflection fluorescence (SW-TIRF).

221 esults confirm the superior resolution of SW-TIRF in addition to the merit of a high signal/backgroun

222 We demonstrate the performance of the SW-TIRF microscopy using one- and two-directional SW illumi

223 expression immobilized GLUT4 vesicles in the TIRF zone and promoted insulin-induced GLUT4 exposure to

224 ion, increased GLUT4 vesicle velocity in the TIRF zone, and prevented their externalization.

225 Reconstruction of the TIRF images enabled 3D imaging of biological samples wit

226 Despite this prominent feature of the TIRF microscope, making quantitative use of this techniq

227 d high-numerical aperture aberrations of the TIRF objective as one important source.

228 is review of FDA documents pertaining to the TIRF REMS, surveys of pharmacists, prescribers, and pati

229 microtubule density and curvature within the TIRF-illuminated region of the cell.

230 Data presented here from real-time TIRF (TIRFM) and confocal microscopy coupled with surfac

231 Here, using cryo-electron tomography, TIRF microscopy, and cell membrane-derived vesicles call

232 ce (TIRF) Forster resonance energy transfer (TIRF-FRET) microscopy allows multiple biomolecules to be

233 the excitation impurities in objective-type TIRF are only weakly affected by changes of azimuthal or

234 simple extension to existing objective-type TIRF microscopes that allows wide-field observations of

235 far-field excitation light in objective-type TIRF, at least for most types of weakly scattering cells

236 n light that is contaminating objective-type TIRF.

237 anted far-field excitation in objective-type TIRF. Pt.1. Identifying sources of nonevanescent excitat

238 We then use TIRF-FRET to monitor the behavior of individual insulin-

239 We use TIRF microscopy to show that cortactin bundles branched

240 tionalized supported lipid bilayers and used TIRF microscopy to follow F, G, and ephrinB2.

241 Here we used TIRF and electron microscopy to directly compare for the

242 We used TIRF microscopy and a statistical method to track and cl

243 We used TIRF microscopy to directly observe actin filament sever

244 Using TIRF microscopy at the single molecule level, transient

245 Using TIRF single-molecule assays, we demonstrate that ScaC in

246 ing of an endogenous protein target by using TIRF microscopy to selectively activate intracellular mo

247 in the plasma membrane of living cells using TIRF microscopy.

248 ents on membrane-bound actin filaments using TIRF microscopy.

249 specific regions of the wave landscape using TIRF microscopy and constitutively active formin constru

250 in vitro analysis of the K118M mutant using TIRF microscopy indicates the actual number of branches

251 also visualized at the cell periphery using TIRF microscopy.

252 alpha6- and beta3-containing receptors using TIRF.

253 asured with single liposome resolution using TIRF microscopy, which allows detection of pore forming

254 Here we show, using TIRF-SIM to examine the organization of microclusters at

255 we developed a single molecule system using TIRF (total internal reflection fluorescence) microscopy

256 near-infrared optical tweezers combined with TIRF microscopy, we were able to trap peroxisomes and ap

257 pectroscopy, particularly in comparison with TIRF.

258 to visualize individual actin filaments with TIRF microscopy, we found that neither flow-induced fila

259 plifier and sandwich immunoassay format with TIRF-FOB.

260 Here, we combine optical trapping with TIRF-based microscopy to measure the force dependence of

261 have combined lateral magnetic tweezers with TIRF microscopy to simultaneously control the restrictiv