ライフサイエンスコーパス: Tn

1 Tn is composed of TnC, TnI, and TnT.

2 Tn-antigen (Tn), a single N-acetylgalactosamine (GalNAc)

3 Tn-Seq is a high throughput technique for analysis of tr

4 Tn-Seq is an experimental method for probing the functio

5 Tn-seq is based on the assembly of a saturated Mariner t

6 Tn-seq measures the frequency of actual members of a het

7 Tn-seq screening of E. coli Tn10 insertion library revea

8 Tn-seq was used to identify genes essential for phototro

9 In the absence of Ca(2+) , Tn binds to actin and constrains Tm to an azimuthal loca

10 In the absence of Ca(2+), Tn that is tightly bound to Tpm binds actin and holds th

11 (WT) Tn, non-phosphorylatable cTnI (S23/24A) Tn or phosphomimetic cTnI (S23/24D) Tn.

12 S23/24A) Tn or phosphomimetic cTnI (S23/24D) Tn.

13 employing 22 million Europeans in a euro 1.5 Tn endeavour, being the premier global economic growth o

14 (6)), to tag and convert Tn to Gal((13)C(6))-Tn, which gives rise to a unique glycan mass.

15 leased at the N-termini of the Gal((13)C(6))-Tn-occupied Ser/Thr residues from immobilized peptides t

16 hmarked by analyzing Jurkat cells, where 947 Tn-glycosylation sites from 480 glycoproteins were mappe

17 C. burnetii clone was isolated containing a Tn insertion within the C terminus of the cell division

18 nTHyp was not evaluated as it did not form a Tn complex under a variety of conditions.

19 One STM mutant, JG736, with a Tn insertion in lpp, encoding Braun's lipoprotein, was c

20 lized B cell line from a male patient with a Tn-syndrome-like phenotype.

21 h may be important in understanding aberrant Tn antigen expression in human diseases, including IgA n

22 In addition, Tn mutants of 14 genes displayed enhanced piglet infecti

23 In the adult Tn environment (cTnT3 + cardiac troponin I), the single

24 ing of the intensity profiles obtained after Tn exchange at pCa 5.8 suggest that the profiles are bes

25 tic efficacy of CAR T cells directed against Tn-MUC1 and present aberrantly glycosylated antigens as

26 also terminal Ser/Thr O-linked alphaGalNAc (Tn antigen).

27 s, characterized by 10-base pair periodic An/Tn-clusters (PATCs), can license transgenes for germline

28 me, indicating a dynamic character to the An/Tn periodicity.

29 cal methods have been proposed for analyzing Tn-Seq data to identify genetic interactions, including

30 te HMM provides an improved way of analyzing Tn-seq data and assessing different levels of essentiali

31 sialylated core 1 O-glycans (T-antigens) and Tn-antigens.

32 novel HexNAc-GalNAc-mucin-type O-glycan, and Tn-antigen; identified the glycosyltransferases for asse

33 gene in mice causes embryonic lethality and Tn antigen expression.

34 We characterize for the first time the T and Tn glycoform O-glycoproteome of the Drosophila melanogas

35 ohydrate antigens, Globo-H, GM2, STn, TF and Tn-to maleimide-modified carrier protein KLH.

36 analysis available for most transposons and Tn-Seq associated approaches (e.g. TraDis, HiTS, IN-Seq)

37 Anti-Tn-MUC1 CAR T cells demonstrated target-specific cytotox

38 (ZPS) PS A1 and the well-known tumor antigen Tn has been designed, synthesized, and studied for immun

39 Tn-antigen (Tn), a single N-acetylgalactosamine (GalNAc) monosacchar

40 Selection with a noncognate human antigen, Tn-MUC1, yielded scFv variants that were broadly reactiv

41 mmon tumor-associated carbohydrate antigens, Tn and sialyl Tn (STn), result from somatic mutations in

42 s of tumor-associated carbohydrate antigens, Tn and STn, were assembled on a single cyclic peptide sc

43 a cluster of the most common TACA (known as Tn antigen) covalently attached to T-cell peptide epitop

44 ngle N-acetylgalactosamine (GalNAc, known as Tn antigen) rather than the normally extended carbohydra

45 in number and location of cancer-associated Tn antigen using the "tea bag" approach.

46 at has specificity for the cancer-associated Tn antigen, to compare the expression of both antigens i

47 9T was unable to recognize cancer-associated Tn epitopes on tumor cell lines.

48 oped a CAR that recognized cancer-associated Tn glycoform of MUC1, a neoantigen expressed in a variet

49 NAc residues present in the tumor-associated Tn antigen (alphaGalNAc-Ser/Thr) and its sialylated form

50 An S. aureus bursa aurealis Tn library consisting of 1,952 non-essential genes was s

51 melanoma-derived cells lines, expressed both Tn and STn antigen due to loss-of-function mutations in

52 f human gut epithelial cells was assessed by Tn-insertion site sequencing (Tn-seq).

53 role of eight additional genes identified by Tn-seq in A. baumannii resistance to killing by NHS but

54 nditionally essential proteins identified by Tn-seq were analyzed by targeted proteomics, and 70% of

55 tion of a cardiac biomarker, troponin I-T-C (Tn I-T-C) complex, was developed.

56 cal model in which strong A-M binding and Ca-Tn binding independently activates the rate of A-M weak-

57 on actin either through calcium-troponin (Ca-Tn) binding or by actin-myosin (A-M) strong binding.

58 Although it is important in cancer, Tn modified glycoproteins are not entirely clear owing t

59 und to be > 2-fold faster from whole cardiac Tn compared with skeletal Tn.

60 or cells exposing both NA2/NA3 and clustered Tn structures.

61 actosamine (GalNAc), the so-called clustered Tn antigen, a cancer-specific O-glycan on mucins.

62 A3-presenting asialofetuin and the clustered Tn-rich asialo-bovine submaxillary mucin, were subsequen

63 n of mouse tumor cells that bear its cognate Tn-glycopeptide epitope in podoplanin, also called OTS8.

64 netic interactions, which involves comparing Tn mutant libraries generated in different genetic backg

65 MAGenTA is a complete Tn-Seq analysis pipeline making sensitive genome-wide fi

66 abeled UDP-Gal((13)C(6)), to tag and convert Tn to Gal((13)C(6))-Tn, which gives rise to a unique gly

67 s work, a transposon insertion mutant (cpsA::Tn) of Mycobacterium marinum was studied.

68 prior to this work, the size of the discrete Tn cluster remained at T3, with only 10 metal sites (e.g

69 ss-reactive toward mucin proteins displaying Tn.

70 rnetii nopA::Tn or a Dot/Icm-defective dotA::Tn mutant strain present a functional innate immune resp

71 Droplet Tn-Seq (dTn-Seq) solves this problem by microfluidics fa

72 At each SL, F(max) was unaffected by either Tn exchange and/or PKA treatment.

73 nge in rigor myofibrils was used to estimate Tn dissociation rates from the nonoverlap and overlap re

74 EXoO-Tn utilizes solid-phase immobilization of proteolytic pe

75 Given the significance of Tn in cancer, EXoO-Tn is anticipated to have broad translational and clinic

76 Here, we introduce a method named EXoO-Tn for large-scale mapping of Tn-glycosylated proteins a

77 The effectiveness of EXoO-Tn was benchmarked by analyzing Jurkat cells, where 947

78 The EXoO-Tn was further applied to the analysis of pancreatic can

79 as tested on simulated data and experimental Tn-Seq data from Serratia marcescens transposon mutant l

80 mutations in Cosmc, and therefore expressing Tn and STn antigens to study the role of O-glycans in TR

81 Guided by the structure of the 237 Fab:Tn-OTS8-glycopeptide complex, here we conducted a deep m

82 Thus far, Tn clusters up to T4 with 20 metal sites can be synthesi

83 In the fetal Tn environment, (cTnT1 + slow skeletal troponin I), the

84 For Tn-seq, a saturated Tn5 insertion library was grown in v

85 olate scFv variants with higher affinity for Tn-OTS8.

86 Novel statistical method for Tn-seq data analysis is needed to infer functions of gen

87 The higher affinities of SBA and VML for Tn-PSM relative to Fd-PSM indicate the importance of car

88 The higher affinities of SBA and VML for Tn-PSM relative to its two shorter chain analogs demonst

89 The resulting data from Tn-seq experiments consist of sequence reads mapped to m

90 gests that Ca(2+) binding to each functional Tn activates < 7 actins of a structural regulatory unit

91 y exposed in the absence of core 1 O-glycan, Tn antigen, in the colon epithelium of a subset of UC pa

92 oma cells often express truncated O-glycans, Tn (GalNAcalpha1-Ser/Thr), and Sialyl-Tn (Siaalpha2-6Gal

93 highly efficient intersystem-crossing S1 --> Tn and phosphorescence T1 --> S0.

94 osamine-transferases (GalNAc-Ts) drives high Tn levels in cancer cell lines and in 70% of malignant b

95 However, Tn-Tx functions in an array of biological processes, and

96 ew studies have compared intermediate and hs-Tn in patients undergoing transcatheter aortic valve rep

97 High-sensitivity Troponin (hs-Tn) has emerged as a useful marker for patients with myo

98 al for core 1 O-glycosylation, were found in Tn-positive epithelia.

99 identifying conditionally essential genes in Tn-Seq experiments with high detection sensitivity and s

100 eletal) muscle, Ca(2+) binding to individual Tn complexes is insufficient to completely activate thei

101 , screened genome-wide transposon insertion (Tn-seq) and transcriptome (RNA-seq) libraries to charact

102 Compared with HCTnT3 (adult isoform), Tn complexes containing HSSTnT1, -2, and -3 did not alte

103 All rod-shaped C. jejuni Tn mutants and all rod-shaped laboratory, clinical and e

104 se activity as a function of pCa and labeled Tn exchange in rigor myofibrils was used to estimate Tn

105 The time course of labeled Tn exchange at pCa 9 and 4 were quite different between

106 However, the synthesis of large Tn clusters is a significant challenge, and for several

107 Mechanistically, Tn/STn antigens impair homo-oligomerization and stabilit

108 high-throughput strategy based on the method Tn-seq that can be applied to any genetically manipulata

109 Here we present a method (Tn-seq) for accurately determining quantitative genetic

110 cultured with Cu, strains containing a mntA::Tn accumulated less Cu than the parent strain.

111 The presence of a mntA::Tn mutation protected iron-sulfur (FeS) enzymes from ina

112 h was abrogated by the introduction of mntA::Tn.

113 The exquisite Gal((13)C(6)) modified Tn are then recognized by a human-gut-bacterial enzyme,

114 eolytic peptides of proteins, which modifies Tn by glycosyltransferase C1GalT1 with isotopically labe

115 These T4 clusters are the largest molecular Tn clusters known to date and can be made in various com

116 elial tumor marker MUC1 carrying one or more Tn, T, or sialyl-T antigens.

117 nts that were broadly reactive with multiple Tn-glycoproteins.

118 n concomitant Ca(2+) binding at neighbouring Tn sites and/or crossbridge feedback effects on Ca(2+) b

119 that cells exposed to the C. burnetii nopA::Tn or a Dot/Icm-defective dotA::Tn mutant strain present

120 reduces costs and retains the advantages of Tn-Seq, while expanding the method's original applicabil

121 is specifically designed for the analysis of Tn-Seq data.

122 An important emerging application of Tn-Seq is for identifying genetic interactions, which in

123 gnificant changes occurred in the binding of Tn containing the RCM cTnT mutation to actin-Tm.

124 alNAc residue along the polypeptide chain of Tn-PSM before dissociating.

125 Comparison of Tn-seq results from laboratory cultures and from monoino

126 erived 81 amino acid tandem repeat domain of Tn-PSM containing approximately 23 alpha-GalNAc residues

127 Expression of Tn and STn in tumor cells attenuates their sensitivity t

128 chaperone Cosmc, favoring the expression of Tn antigen.

129 of cancer associated with the expression of Tn.

130 apoptotic stimuli, suggesting expression of Tn/STn may offer tumor cell survival advantages through

131 Ag/AgCl reference electrode as a function of Tn I-T-C complex concentration during incubations.

132 egy that incurs significant incorporation of Tn antigen.

133 Inhibition of Tn-Tx potentially offers a new therapeutic intervention

134 ost malignant tumors have elevated levels of Tn, an O-GalNAc glycan.

135 hod named EXoO-Tn for large-scale mapping of Tn-glycosylated proteins and glycosylation sites.

136 ine, which could be considered as a mimic of Tn antigen.

137 Our results highlight the power of Tn-seq for the discovery of new genes and novel pathways

138 m motility that persisted in the presence of Tn and submaximal Ca(2+) Furthermore, decreasing the ext

139 This report reveals the protective role of Tn fetal isoforms as they rescue the increased Ca(2+) se

140 ived 38/40 amino acid cleavage product(s) of Tn-PSM containing approximately 11-12 alpha-GalNAc resid

141 Given the significance of Tn in cancer, EXoO-Tn is anticipated to have broad trans

142 Depending on the activation state of Tn and the binding state of myosin, Tm can occupy the bl

143 nin (Tn) isoform expression and/or status of Tn phosphorylation, we studied fiber bundles that had be

144 idue-specific consequences of acetylation on Tn-Tpm-based regulation of actomyosin activity, we asses

145 timulation of cell migration is dependent on Tn-bearing proteins present in lamellipodia of migrating

146 An ordered Tn library in strain AB5075 with insertions in every non

147 However, the affinity of bound SBA for other Tn-PSM molecules during cross-linking is much higher tha

148 ty of bound SBA for GalNAc residues on other Tn-PSM molecules appears to be due to the favorable entr

149 estyle to bloodstream invasion, we performed Tn-seq on GBS strain A909 with human whole blood.

150 A controls the expansion of IL-17A-producing Tn cell populations through IL-17R.

151 ecretion, and the number of IL-17A-producing Tn cells were elevated in Il17ra(-/-) and Il17ra(-/-)Itg

152 The total number of CD3+ IL-17A-producing Tn cells were significantly reduced in the spleen and la

153 Me, L = Cl(-), NCS(-), NCO(-), N(3)(-); R' = Tn, L = Cl(-), NCS(-).

154 e endogenous cTn was replaced by recombinant Tn.

155 y expressed fast skeletal muscle recombinant Tn.

156 s, restores T-synthase activity, and reduces Tn antigen expression.

157 ough IL-17A-producing neutrophil regulatory (Tn) cells, most of which express gammadelta TCR.

158 show that expression of the disease-related Tn antigen can result from deregulation or loss of Cosmc

159 nd approximately 2300 alpha-GalNAc residues (Tn-PSM) binds to the soybean agglutinin (SBA) with a K(d

160 mately 2300 GalNAcalpha1-O-Ser/Thr residues (Tn-PSM) has been ascribed to an internal diffusion mecha

161 d be regarded as conformationally restricted Tn antigen mimics, as we have demonstrated by biological

162 binds MUC1 that carries the Tn or sialyl (S)Tn glycan.

163 s gamma-globin gene were achieved using a SB-Tn beta-globin cis construct.

164 suggested that a fluorescent reporter cis SB-Tn system can be used to enrich mammalian cells harborin

165 ere a novel dual fluorescent reporter cis SB-Tn system that permitted nonselective fluorescent-activa

166 We then constructed cis SB-Tn-beta-globin plasmids using a minimal beta-globin gene

167 ant enrichment (>60%) of cells exhibiting SB-Tn-mediated genomic insertions and long-term expression

168 ve fluorescent-activated cell sorting for SB-Tn-transduced K562 erythroid cells.

169 The SB-Tn system is a promising nonviral vector for efficient g

170 Sleeping Beauty transposon (SB-Tn) has emerged as an important nonviral vector for inte

171 Using a high-throughput screen (Tn-seq), we identified genes in recipients that contribu

172 ere assessed using ToxT and in vivo RNA-seq, Tn-seq, and cholera stool proteomic and other genome-wid

173 transposon mutagenesis and deep sequencing (Tn-seq) to identify T6SS immunity proteins in Vibrio cho

174 s and a new transposon insertion sequencing (Tn-Seq) dataset that we generated.

175 Transposon insertion sequencing (Tn-Seq) is a microbial systems-level tool, that can dete

176 Transposon insertion sequencing (Tn-seq) is an emerging technology that combines transpos

177 We used transposon insertion sequencing (Tn-Seq) to define essential genes in nine strains of Pse

178 e used transposon insertion site sequencing (Tn-seq) to comprehensively assess the contribution of ne

179 as assessed by Tn-insertion site sequencing (Tn-seq).

180 next-generation high-throughput sequencing (Tn-seq) promises to revolutionize systems level analysis

181 um resistance, a transposon (Tn) sequencing (Tn-seq) approach was used to identify genes contributing

182 genetic screen with a transposon sequencing (Tn-seq) library of a pneumococcal strain in a ferret tra

183 previously reported a transposon sequencing (Tn-seq) system for performing genomewide assessment of g

184 A screen using transposon sequencing (Tn-seq) was performed to search for genes within ExPEC i

185 re published in 2009: transposon sequencing (Tn-Seq), transposon-directed insertion site sequencing (

186 eutropenic mice using transposon sequencing (Tn-seq).

187 Here, we used transposon-sequencing (Tn-seq) to assess whether there were any sporulation gen

188 tory factors, we used transposon-sequencing (Tn-Seq)(5) to screen for mutations affecting the growth

189 ced affinity relative to GalNAcalpha1-O-Ser (Tn), the pancarcinoma carbohydrate antigen.

190 ociated carbohydrate antigens, Tn and sialyl Tn (STn), result from somatic mutations in the gene Cosm

191 ycans, Tn (GalNAcalpha1-Ser/Thr), and Sialyl-Tn (Siaalpha2-6GalNAcalpha1-Ser/Thr, STn) on their surfa

192 scrimination of the cancer-associated sialyl-Tn (STn) antigen was developed by using Sambucus nigra a

193 corporation into the carcinoma marker Sialyl-Tn, and is the first example of such a novel mechanism f

194 Ser/Thr) and its sialylated form, the sialyl-Tn antigen.

195 9T mutant failed to interact with the sialyl-Tn epitope.

196 recombinant Tm mutants and purified skeletal Tn.

197 from whole cardiac Tn compared with skeletal Tn.

198 Small Tn clusters can also be synthesized in discrete forms, a

199 C-ms proteomics), gene essentiality studies (Tn-seq), genome wide protein localization studies, and g

200 We present herein several sulfa-Tn antigens incorporated in MUC1 sequences that possess

201 Supertetrahedral Tn clusters are exact fragments of a cubic ZnS type latt

202 Supertetrahedral Tn clusters are exact fragments of cubic ZnS-type lattic

203 sents the largest molecular supertetrahedral Tn cluster known to date.

204 mmune responses derived from single naive T (Tn) cells, single primary, and single secondary central

205 f IL-17A-expressing neutrophil-regulatory T (Tn) cells; CD4(-)CD8(-)alphabeta(low), CD4(+)CD8(-)alpha

206 ocked down in ESCC cell lines (KYSE450 and T.Tn), immortalized normal esophageal epithelial cell line

207 The Tn complex consists of three subunits, troponin C (TnC),

208 The Tn-PS A1 conjugate construct confers specificity toward

209 ero-inflated Poisson model for analyzing the Tn-seq data that are high-dimensional and with an excess

210 In cancer cells, some glycans (such as the Tn antigen) are highly up-regulated, but this remains la

211 n with terminal GalNAc moieties, such as the Tn antigen, on CD45 of effector T cells, thereby down-re

212 tures of MUC1-like glycopeptides bearing the Tn antigen (alpha-O-GalNAc-Ser/Thr) in complex with an a

213 CAR selectively binds MUC1 that carries the Tn or sialyl (S)Tn glycan.

214 59) mediates H bonds directly or engages the Tn-glycopeptide backbone through water molecules.

215 lls lack T-synthase activity and express the Tn antigen.

216 ies of mucins including those expressing the Tn cancer antigen.

217 onal scan showing that residues flanking the Tn-glycan contributed significant binding energy to the

218 of a scFv antibody fragment specific for the Tn-glycoform of MUC1 had potent activity in preclinical

219 ase, the only enzyme that galactosylates the Tn antigen (GalNAcalpha1-Ser/Thr-R) to form core 1 Galbe

220 g synthetic glycopeptides with O-GalNAc (the Tn antigen) or O-GlcNAc, we demonstrated that the method

221 Knowing the Tn-glycosylated proteins and glycosylation sites are ess

222 T-synthase and consequent expression of the Tn antigen (GalNAcalpha1-Ser/Thr), which is associated w

223 sm underlying the abnormal expression of the Tn antigen, which may be important in understanding aber

224 N-lobe to rotate relative to the rest of the Tn molecule.

225 A polymerizable version of the Tn-antigen glycan was prepared and converted into well-d

226 A special feature of the Tn-seq data is that multiple mutants in a gene provides

227 ncer specimens that showed expression of the Tn/STn antigens were also found to have mutations in Cos

228 osin ATPase activity than that of TnC or the Tn complex.

229 ts suggest that MGL not only connects to the Tn carbohydrate epitope, but also engages the underlying

230 tion of antibodies that are selective to the Tn-antigen glycan and cross-reactive toward mucin protei

231 ate construct confers specificity toward the Tn antigen alone, and specific carbohydrate immunoglobul

232 The analysis used the Tn-seq circle method to achieve high genome coverage and

233 the identification of glycopeptides with the Tn antigen by mass spectrometry.

234 hod, we identified 96 glycoproteins with the Tn antigen in Jurkat cells.

235 st designed to enrich glycoproteins with the Tn antigen.

236 ion (R = tert-butyl, R' = H, Me, 2'-thienyl (Tn), L = Cl(-), NCS(-), NCO(-), N(3)(-)), has been chara

237 riation and morphological trends within this Tn library, and in various C. jejuni wild type strains,

238 lence determines the affinities of the three Tn-PSM analogs.

239 time course of myosin-S1 binding to actin-Tm-Tn filaments in solution at various calcium levels with

240 In addition to Tn-actin interactions, inhibitory Tm positioning require

241 Our approach was applied to Tn-Seq libraries made in isogenic strains of Mycobacteri

242 Ca(2+) binding to Tn releases the Tpm from actin so that it moves azimutha

243 le contraction by coupling Ca(2+) binding to Tn with myosin binding to the thin filament.

244 ing negative cooperativity of SBA binding to Tn-PSM correlates with a decreasing level of internal di

245 investigated its binding characteristics to Tn-containing glycopeptides derived from the MGL ligands

246 9T variant lost high-affinity binding toward Tn-containing glycopeptides, especially at low probing c

247 Transposon (Tn) gene-inactivation libraries were generated in three

248 Of 3,850 transposon (Tn) mutants screened, 46 were identified as colonization

249 pC, we identified mutants from a transposon (Tn) insertion library which lack surface-exposed Ebp pil

250 We screened a transposon (Tn) mutant library in the cop- background and isolated s

251 strointestinal tract utilising a transposon (Tn) mutant library screen.

252 A. baumannii serum resistance, a transposon (Tn) sequencing (Tn-seq) approach was used to identify ge

253 us, we screened a low-complexity transposon (Tn) mutant library to identify novel genes important for

254 enerated by mariner-based Himar1 transposon (Tn) mutagenesis.

255 ced by technological advances in transposon (Tn) mutagenesis.

256 A C. jejuni transposon (Tn) mutant library was screened for non-helical mutants

257 d potential drug targets, random transposon (Tn) mutants derived from the A. baumannii strain AB307-0

258 system, the Sleeping Beauty (SB) transposon (Tn) to insert a wild-type beta-globin expression cassett

259 the wide activity of the Mariner transposon, Tn-seq has the potential to contribute to the exploratio

260 ssing miRNA precursors by the Translin-Trax (Tn-Tx) ribonuclease.

261 generated an S. aureus ddh/ldh1/ldh2 triple Tn mutant that cannot produce D- or L-lactate.

262 t least in part by the troponin-tropomyosin (Tn-Tm) switch on the thin (actin-containing) filaments.

263 Troponin (Tn) is an important regulatory protein in the thin-filam

264 Troponin (Tn) is the calcium-sensing protein of the thin filament.

265 nd Lethocerus tropomyosin (Tm) and troponin (Tn) regulate the binding of rabbit myosin to rabbit acti

266 ils by replacing endogenous Tm and troponin (Tn) with recombinant Tm mutants and purified skeletal Tn

267 , myosin-S1, tropomyosin (Tm), and troponin (Tn).

268 en years ago, mutations in cardiac troponin (Tn)T and alpha-tropomyosin were linked to familial hyper

269 force-pCa relationship, endogenous troponin (Tn) was exchanged in rat ventricular trabeculae with eit

270 ng to F-actin by the thin filament troponin (Tn)-tropomyosin (Tm) complex.

271 To rule out any alterations in troponin (Tn) isoform expression and/or status of Tn phosphorylati

272 In skeletal and cardiac muscles, troponin (Tn), which resides on the thin filament, senses a change

273 uence data suggest the presence of troponin (Tn) components, consistent with actin-linked regulation,

274 The inhibitory region of troponin (Tn)I differs by a single residue, proline at position 11

275 ding and dissociation of Ca(2+) on troponin (Tn) to the movement of tropomyosin on actin filaments.

276 Ca(2+) via the regulatory proteins troponin (Tn) and tropomyosin (Tpm), which are associated with act

277 The troponin (Tn) complex also influences Tpm's position along F-actin

278 ase the calcium sensitivity of the troponin (Tn) complex or reconstituted thin filaments with or with

279 ning of Tm along the actin (Ac):Tm:troponin (Tn) thin filament to block or expose myosin binding site

280 Mechanisms underlying Tn up-regulation and its effects remain unclear.

281 on consisting of sialylated and unsialylated Tn-antigens and core 1 and core 2 structures.

282 Here we use Tn-seq to assess gene function in the Gram negative gamm

283 Here, we used Tn-seq to identify genes important for resistance to com

284 By using Tn-Seq, RNA-Seq, microarray and proteomics datasets from

285 ysis of a saturated mutant GAS library using Tn-sequencing and generation of a conditional-expression

286 sequenced in a high throughput manner using Tn-seq to identify potential mechanisms of xds regulatio

287 using Sanger sequencing and in a pool using Tn-seq.

288 on bottlenecks in a murine host, we utilized Tn-seq to monitor the composition of mixed populations o

289 imilar reduction also could be observed when Tn contained cTnI(Thr>Pro) (deltaEC50=0.24+/-0.04 microm

290 he analysis of pancreatic cancer sera, where Tn-glycoproteins were identified.

291 A total of 123 genes of which Tn mutants showed attenuated piglet infection were ident

292 While Tn-Seq is a powerful tool to determine genome-wide bacte

293 a(2+) dissociation rates (k(off)) from whole Tn complexes containing sTnC (26 +/- 0.1 s(-1)), cTnC (3

294 This strategy provides CARs with Tn-peptide specificities, all based on a single scFv sca

295 vors exhibited abnormalities correlated with Tn antigen expression that are related to several human

296 A murine SCD model coupled with Tn-seq mutagenesis identified 60 noncapsular pneumococca

297 ated form of Cosmc observed in patients with Tn syndrome has reduced chaperone function.

298 he cop- background and isolated strains with Tn insertions in the mntABC operon that permitted growth

299 function, their subunit interactions within Tn and with actin-tropomyosin are different.

300 icular trabeculae with either wild-type (WT) Tn, non-phosphorylatable cTnI (S23/24A) Tn or phosphomim