ライフサイエンスコーパス: Triton X-100

1 pon the addition of nonionic detergent (0.1% Triton X-100).

2 of Jurkat cells in the presence of the agent Triton X-100).

3 +), O 2, alpha-ketoglutarate, ascorbate, and Triton X-100.

4 t is extracted from low density fractions by Triton X-100.

5 olubilized from frozen RBC by addition of 1% Triton X-100.

6 nel is reversibly inhibited by the detergent Triton X-100.

7 the presence of a sublethal concentration of Triton X-100.

8 s reactive on extraction of the protein into Triton X-100.

9 ective to facilitate CeO2-NPs transport than Triton X-100.

10 microdomains (DRMs) were extracted with cold Triton X-100.

11 GPI(+) PrP-sen resisted extraction with cold Triton X-100.

12 ral elasticity and resists solubilization by Triton X-100.

13 d resistance of the patches to extraction by Triton X-100.

14 n levels if cultured in the presence of 0.1% Triton X-100.

15 be reversed or prevented by the addition of Triton X-100.

16 ions of the host cell and was extractable by Triton X-100.

17 nce of high levels of salt and the detergent Triton X-100.

18 g of the GEM domains and their resistance to Triton X-100.

19 constructs for resistance to extraction with Triton X-100.

20 at pH 8 and is inhibited by 0.01% (or more) Triton X-100.

21 s, which were isolated in the presence of 1% Triton X-100.

22 f cell monolayers or tissue homogenates with Triton X-100.

23 acrylate combined with a nonionic surfactant Triton X-100.

24 ed iron oxidation, and was also sensitive to Triton X-100.

25 ut are disrupted in harsh detergents such as Triton X-100.

26 teinase K only when exosomes were exposed to Triton X-100.

27 e removed effectively by nonionic surfactant Triton X-100.

28 H 7), aqueous, micellar solutions of reduced Triton-X 100.

29 ase activity required Mg(2+) ions (8 mm) and Triton X-100 (0.25 mm) at pH 5.0.

30 Maximum PAP activity was dependent on Triton X-100 (20 mm), PA (2 mm), Mg(2+) (0.5 mm), and 2-

31 r the homogenization of the oil samples with Triton X-100, 200 muL of methanol was added to facilitat

32 les composed of 10% anionic phospholipids in Triton X-100 a monodisperse protein-lipid complex is for

33 When 0.1% reduced Triton X-100, a detergent that more closely simulates th

34 Conversely pre-extraction in Triton X-100, a treatment that promotes SNARE complexes

35 We utilize purified Triton-X 100, a nonionic surfactant, to make soap bubble

36 Triton X-100 also thickens Lo domains, but partitions to

37 g in methanol, we report that the surfactant Triton-X 100 also yields a large absorbance or fluoresce

38 The protein was solubilized with 1% Triton X-100 and 0.5 M sodium chloride and then purified

39 of these observations, we used a mixture of Triton X-100 and 1-butanol and observed that water-solub

40 ne/HCl, pH 7, buffer containing 0.025% (w/v) Triton X-100 and 15 mM MgCl(2) provided optimum conditio

41 % or 1% sodium dodecyl sulfate (SDS) or 0.1% Triton X-100 and assayed for clinical chemistry and mala

42 The subsequent introduction of Triton X-100 and CoQ10 causes the MLs lysis and the cres

43 amprenavir, and the Envs were solubilized in Triton X-100 and isolated by sedimentation in a sucrose

44 h medium and hypersensitive to the detergent Triton X-100 and multiple antibiotics.

45 Milk sample slurries were prepared using Triton X-100 and nitric acid for direct analysis of Pb u

46 mide; and importantly, low concentrations of Triton X-100 and other non-ionic detergents were strongl

47 anced autolysis and increased sensitivity to Triton X-100 and penicillin, attributable in part to inc

48 The sarV mutant was more resistant to Triton X-100 and penicillin-induced lysis compared to th

49 emicellar aggregation of the DiC(6)SNPE with Triton X-100 and PLA(2).

50 The protein was solubilized with Triton X-100 and purified to near-homogeneity.

51 en perfused with PBS, followed by successive Triton X-100 and SDS solutions in saline buffer.

52 In Triton X-100 and sodium cholate solutions, the aggregate

53 SDS, Brij-35, Brij-58, Triton X-100 and Span-40 were explored for the extractio

54 s dependent on the presence of the detergent Triton X-100 and the methyldonor S-adenosylmethionine.

55 d on a single cryo-cooled crystal grown with Triton X-100 and the structure was solved by molecular r

56 KCl solution containing nonionic surfactant Triton X-100 and their translocation was studied at diff

57 In aqueous solution of nonionic surfactants (Triton X-100 and Tween 20) arrays from the second series

58 d from HeLa-Cx43(His)(6)/Cx45 cells by using Triton X-100 and were applied to a Ni(2+)-NTA column, wh

59 s (donor ages, 69-84 years) and treated with Triton X-100 and/or coated with a mixture of laminin (33

60 onorhamnolipid was compared to that of SDBS, Triton X-100, and ethanol.

61 ocked with cis-Golgi, were solubilized in 2% Triton X-100, and proteins were immunoprecipitated using

62 pilQ also resulted in reduced entry of heme, Triton X-100, and some antibiotics.

63 moderate concentrations, the activity of the Triton X-100- and octylglucoside-solubilized material co

64 gh concentrations of inorganic ions, using a Triton X-100 aqueous solution to dilute the sample durin

65 ted as detergent concentrations (Tween 80 or Triton X-100) are increased up to their critical micelle

66 ap the interface of the Bax oligomer we used Triton X-100 as a membrane surrogate and performed site-

67 duction of micellar organised media by using Triton X-100 as an extraction solvent.

68 nd basolateral plasma membrane domains using Triton X-100 as detergent, and characterized their lipid

69 the CPD transmembrane domain was soluble in Triton X-100 as was endogenous palmitoylated CPD, indica

70 Bovine ROS membranes were incubated with 1% Triton X-100 at 4 degrees C and subjected to density gra

71 After lysis with Triton X-100 at 4 degrees C and sucrose linear-density c

72 on of cells expressing the F protein with 1% Triton X-100 at 4 degrees C, ca. 40% of total, cell-asso

73 insolubility in nonionic detergents such as Triton X-100 at 4 degrees C.

74 e are solubilized by lower concentrations of Triton X-100, at least within certain temperature ranges

75 ondrial membranes contains up to 120 nmol of Triton X-100 bound per nanomole of the enzyme.

76 Removal of Triton X-100 bound to bovine cytochrome bc(1) was accomp

77 el resistance to HAs (e.g., erythromycin and Triton X-100) but only a twofold increase in resistance

78 rmediates are disrupted by solubilization in Triton X-100, but chemical cross-linking stabilizes a pu

79 s was more easily perturbed by the detergent Triton X-100, but not other non-ionic detergents.

80 by dilute buffer, high salt concentrations, Triton X-100, Ca(2+)-dipicolinic acid, dithiothreitol, o

81 (pH 7.6) containing 0.1 M NaCl and 1% (v/v) Triton X-100 caused liberation of the Ru(bpy)32+ from th

82 sucrose gradient ultracentrifugation of cold Triton X-100 cell lysates yielded both CD36 and caveolin

83 Western blot analysis of Triton X-100 cold-extracted membrane fractions confirmed

84 e complexation and extraction (pH, DDTC, and Triton X-100 concentration, vortex agitation time and co

85 dependent binding of cytoplasmic proteins to Triton X-100 cytoskeletons of L-929 cells grown on colla

86 , these GPC microdomains are soluble in cold Triton X-100 detergent and are thus distinct from conven

87 WT beta2 subunits are resistant to live cell Triton X-100 detergent extraction from the hippocampal a

88 activity by approximately 70% and abolished Triton X-100 detergent inhibition of Ca-dependent nucleo

89 sites maintained 'lipid rafts' as defined by Triton X-100 detergent resistant membrane formation.

90 glycosylation status, expression level, and Triton X-100 detergent sensitivity.

91 ions containing BIG/TIR3 and partitions into Triton X-100 detergent-resistant membrane (DRM) fraction

92 e Nephrin and Podocin, Neph1 was enriched in Triton X-100 detergent-resistant membrane fractions.

93 A protocol to prepare Triton X-100 detergent-resistant membranes (DRMs) was de

94 that of monomeric BR solubilized in reduced Triton X-100 detergent.

95 nfirmed by TEM, treatment of sperm with 0.2% Triton X-100 disrupted the plasma, acrosomal, and inner

96 We demonstrated that Triton X-100 dissociated the heptameric complex into thr

97 ECM by detergent decellularization methods (Triton X-100, DOC and SDS are compared).

98 Treatment of blood samples with 0.1% SDS or Triton X-100 does not inactivate EBOV.

99 ced in the presence of low concentrations of Triton X-100 either by brief exposure to 80 degrees C or

100 nol gave slightly better results than when a Triton X-100-ethanol solution was used for dilution.

101 all TM layers, but not in tissues killed by Triton X-100 exposure.

102 liposomes generated from a flippase-enriched Triton X-100 extract of ER can flip analogues of phospha

103 By using cold Triton X-100 extractability of different lipids as a cri

104 Triton X-100 extracted approximately 10% of the cellular

105 phalloidin staining and Western blotting of Triton X-100 extracted cell lysate.

106 O flipping in proteoliposomes generated from Triton X-100-extracted Saccharomyces cerevisiae microsom

107 was found to be largely insoluble following Triton X-100 extraction and cofractionationed with bioch

108 r localization of CLIC-5A was retained after Triton X-100 extraction and was disrupted by treatment w

109 S disk membrane was found to be resistant to Triton X-100 extraction at 4 degrees C.

110 this fraction was resistant to high salt and Triton X-100 extraction at pH 6.5.

111 Experiments using Triton X-100 extraction followed by OptiPrep density gra

112 is showed that full-length LEDGF/p75 resists Triton X-100 extraction from chromatin.

113 We now demonstrate by Triton X-100 extraction immunoprecipitation and in vitro

114 Triton X-100 extraction of cardiac muscle fibers promote

115 with CKI-7, and Cx43 content was analyzed by Triton X-100 extraction, cell-surface biotinylation, and

116 ng the cell membrane and soluble proteins by Triton X-100 extraction, we found that the remaining com

117 teins CD4, CXCR4, and CCR5, as determined by Triton X-100 extraction.

118 t disrupted by hypotonic treatment and brief Triton X-100 extraction.

119 d, at least at 4 degrees C, are resistant to Triton X-100 extraction.

120 We observed that Triton X-100 extracts of N. gonorrhoeae strain F62 conta

121 rast to assays utilizing intact membranes or Triton X-100 extracts, assays using CHAPS- or tDOC-washe

122 l membranes were extracted sequentially with Triton X-100, followed by DNase I or potassium iodide to

123 Permeabilization with Triton X-100 for 60 min also abolished hypoxic relaxatio

124 The resulting Triton X-100-free cytochrome bc(1) retained nearly full

125 rthermore, GPC was more readily extracted by Triton X-100 from adenosine triphosphate (ATP)-depleted

126 ure, buffered at pH 7.8 and containing Tris, Triton X-100, glucose-6-phosphate, nicotinamide adenine

127 to protein extraction and reconstitution in Triton X-100/glycerol-permeabilized smooth muscle fibers

128 to a butyl Sepharose column and eluted in a Triton X-100 gradient.

129 ta activity was abolished by the denaturants triton X-100, Gua-HCl, Gua-thiocyanate, SDS and urea in

130 ed IC50 values of three cytotoxic chemicals, Triton X-100, H2O2, and cadmium chloride, as model compo

131 A mixture of phospholipids and Triton X-100 in a molar ratio of 5:1 forms well-aligned

132 turing detergent perfluoro-octanoic acid and Triton X-100 in combination with gel filtration, sucrose

133 s largely relieved by the inclusion of 0.01% Triton X-100 in the assay solutions, which has been sugg

134 by sodium dodecyl sulfate, Nonidet P-40, or Triton X-100 in the mass spectrometry analysis.

135 is opposite to the observed orientation for Triton X-100 in the SCP-2-like domain from the peroxisom

136 e neuroglian is resistant to extraction with Triton X-100 in the sorting zone and nerve layer, possib

137 mphiphiles, sodium dodecyl sulfate (SDS) and Triton X-100, in addition to a similar synthesized dendr

138 TcGP63 is soluble in cold Triton X-100, in contrast to Leishmania GP63, which is d

139 tion of cardiac myocytes with saponin and/or Triton X-100 increased NAADP synthesis, indicating that

140 as resistant to solubilization with urea and Triton X-100, indicating the formation of larger tau agg

141 erythrocytes were more easily extractable by Triton X-100, indicating weaker association to the cytos

142 of USA300 was found to be more resistant to Triton X-100-induced autolysis and also to lysis by lyso

143 on of gcp expression can effectively inhibit Triton X-100-induced lysis, eliminate penicillin- and va

144 otidase activities while greatly attenuating Triton X-100 inhibition of Mg-dependent nucleotidase act

145 mple dilution with an OIS and the surfactant Triton X-100 (inorganic media) or ethanol (organic media

146 pid raft association, using as the criterion Triton X-100 insolubility, loss of raft association did

147 ls; like HMW2, HMW2Deltamid exhibited marked Triton X-100 insolubility.

148 with the actin cytoskeleton, we isolated the Triton X-100 insoluble actin cytoskeleton from platelets

149 We also found similar Triton X-100 insoluble forms of alpha-synuclein in an al

150 isfolded and aggregated alpha-synuclein in a Triton X-100 insoluble fraction as well as a high molecu

151 IL-11 induced a shift of hsp25 to Triton x-100 insoluble fractions containing cytoskeletal

152 cking the N-terminal domain, was detected in Triton X-100 insoluble fractions in Western blot analysi

153 istribution was characterized by analysis of Triton x-100 insoluble fractions, 2-D isoelectric focusi

154 lted in a redistribution of PrP(C) away from Triton X-100 insoluble lipid rafts.

155 was associated almost exclusively with 0.1% Triton X-100 insoluble material, consistent with its sig

156 ibited by DP-S2849G-GFP in the cytoskeletal (Triton X-100 insoluble) fraction, and keratin filament r

157 lated (activated) EGFR was found only in the Triton X-100-insoluble (lipid raft) fraction, whereas to

158 butes to the edge of the lamellae and to the Triton X-100-insoluble actin cytoskeleton of activated W

159 ither WTsyn or A53Tsyn led to a reduction in Triton X-100-insoluble aggregates and an increase in pro

160 Expression of 16 kDa also resulted in a Triton X-100-insoluble aggregation of an unusual 87-kDa

161 localized in Triton X-100-soluble as well as Triton X-100-insoluble cell fractions.

162 Flightless-I targets caspase-11 to the Triton X-100-insoluble cytoskeleton fraction and the cel

163 Triton X-100-insoluble cytoskeletons were stretched by 1

164 tin and moesin and was found enriched in the Triton X-100-insoluble fraction along with p67(phox) and

165 searched for Dyrk1A binding proteins in the Triton X-100-insoluble fraction extracted with urea and

166 ome isoforms being enriched primarily in the Triton X-100-insoluble fraction in lens tissue.

167 ounts of all three proteins increased in the Triton X-100-insoluble fraction of cell lysates and decr

168 GFP-CFTR localized to low density Triton X-100-insoluble fractions in lysates of Madin-Dar

169 The Triton X-100-insoluble fractions of M. penetrans and M.

170 Both receptors were excluded from Triton X-100-insoluble lipid rafts, and at high agonist

171 brain NgR1 and NgR2 are strongly enriched in Triton X-100-insoluble lipid rafts.

172 c tail abrogated the association of BHA with Triton X-100-insoluble lipid rafts.

173 re spermatozoa, caveolin-1 was enriched in a Triton X-100-insoluble membrane fraction, as well as in

174 fts, Triton X-100-soluble fractions, and the Triton X-100-insoluble pellet following apical infection

175 Flotation of the cold Triton X-100-insoluble portion of membranes on sucrose g

176 post-translationally converted into a 20-kDa Triton X-100-insoluble proteasome substrate.

177 The amount of endogenous p130Cas in the Triton X-100-insoluble protein fraction, and fibronectin

178 redistribution of Kir2.1 and Kv2.1 from the Triton X-100-insoluble to the Triton X-100-soluble membr

179 Triton X-100 is employed at finely tuned concentrations

180 ether dodecyl maltoside, decyl maltoside, or Triton X-100 is the primary solubilizing detergent or th

181 t when the apposed bilayers are dissolved in Triton X-100; it is also observed during fusion of isola

182 to nonionic micelles in the running buffer (Triton X-100), linking the tagged DNA to the micellar dr

183 Density gradient centrifugation of Triton X-100 lysates from B lymphocytes demonstrated tha

184 radient analysis of plasma membrane-labeled, Triton X-100-lysed cells shows that proximity measured b

185 non-detergent or detergent-based (saponin or Triton X-100) methods.

186 t interact in the hetero-complex formed in a Triton X-100 micelle as a membrane surrogate.

187 ha (>15-fold) with a K(a) of 2.4 mol % C-1-P/Triton X-100 micelle.

188 We also show that smaller Triton X-100 micelles give a read length of 103 bases in

189 Arabidopsis SphK with Sph presented in mixed Triton X-100 micelles indicated that SphK associates wit

190 zyme activity in a cell-free system using PA/Triton X-100 mixed micelles as substrate, analyzing it i

191 Binding of DiC(6)SNPE when presented as Triton X-100 mixed micelles saturated at 0.93 binding si

192 centrations at all surface concentrations in Triton X-100 mixed micelles.

193 hange in the lipin 1beta affinity for the PA/Triton X-100 mixed micelles.

194 The nonionic detergents Triton X-100, Nonidet P-40, Brij, Tween, and octylglucos

195 mbrane expressing CFTR in detergents such as Triton X-100, Nonidet P-40, deoxycholate, and SDS tended

196 o]-1-propanesulfonate (CHAPS, zwitterionic), Triton X-100 (nonionic), sodium dodecyl sulfate (SDS, an

197 d point temperature of non-ionic surfactant, Triton X-100 occurred and complex was entrapped in surfa

198 in a highly purified plant PSII preparation (Triton X-100, octylthioglucoside).

199 We have studied the solubilization by Triton X-100 of binary mixtures composed of egg sphingom

200 ticity because the micelle-forming detergent Triton X-100 only minimally affects TRPV1 properties.

201 LpxE activity is dependent upon Triton X-100, optimal near pH 6.5, and Mg2+-independent.

202 Perturbations caused by Triton X-100 or mutations convert faster to slower forms

203 were obtained if extraction media contained Triton X-100 or sodium dodecyl sulfate.

204 les (e.g., diheptanoylphosphatidylcholine or Triton X-100) or 0.1-0.2 M inorganic salts.

205 %, but it was not inhibited by Nonidet P-40, Triton X-100, or octyl glucoside, even at concentrations

206 Analysis of surface dilution kinetics with Triton X-100/PA-mixed micelles yielded constants for sur

207 of 4.2, 4.5, and 4.3 mol %, respectively) in Triton X-100/PA-mixed micelles.

208 Confocal microscopy of Triton X-100 permeabilized neutrophils showed that a sma

209 Triton X-100-permeabilized proplatelets containing dynei

210 In this study, a Triton X-100/phosphatidylcholine (PC) mixed micelle assa

211 centration of substrate phospholipids in the Triton X-100/phospholipid mixed micelles.

212 Platelet-derived FV/Va, purified from Triton X-100 platelet lysates was composed of a mixture

213 The effects of the nonionic surfactant Triton X-100 (poly(ethylene glycol) tert-octylphenyl eth

214 When lipid vesicles were disrupted by Triton X-100, PrP aggregation was necessary to maintain

215 inner and outer leaflet lipids, whereas the Triton X-100 rafts contained a preponderance of outer le

216 lin than did the non-EGF receptor-containing Triton X-100 rafts.

217 The Triton X-100 releases the cytoplasmic contents into the

218 opy, SCD overexpression led to a decrease of Triton X-100-resistant domains in the plasma membrane, i

219 Analysis of proteins associated with the Triton X-100-resistant fraction showed it to be enriched

220 Biochemical analysis of the Triton X-100-resistant fraction showed it to be enriched

221 ll of the Cx43 in G0 cells is assembled into Triton X-100-resistant junctions, Cx43-ZO-1 interaction

222 orts the isolation and characterization of a Triton X-100-resistant membrane fraction from homogenate

223 t with EGCG caused a marked reduction in the Triton X-100-resistant membrane fraction.

224 to cellular membranes but are not present in Triton X-100-resistant membrane rafts.

225 The Triton X-100-resistant membranes described herein did no

226 class V myosin isoforms are associated with Triton X-100-resistant membranes isolated from mouse for

227 When the Triton X-100-resistant membranes were treated with methy

228 The lipid profiles of the Triton X-100-resistant membranes were virtually identica

229 (QRD(194-196)) is required for strong (i.e., Triton X-100-resistant) alpha 3 beta 1 association and f

230 d Brij 98-resistant) and those that did not (Triton X-100-resistant), we used multidimensional electr

231 7-s incubation times were 290 and 250 microM Triton X-100, respectively.

232 was diluted and homogenised by n-hexane and Triton X-100, respectively.

233 ation of transfected cells with digitonin or Triton X-100, respectively.

234 Addition of submicellar amounts of Triton X-100 resulted in a linear nonsaturating response

235 derivatives in aqueous solutions of reduced Triton X-100 (RTX-100) were determined by measurements o

236 th itself, integrins, or other tetraspanins (Triton X-100-sensitive but Brij 96-resistant) were indep

237 sodium dodecyl sulfate (SDS), Lubrol PX, or Triton X-100, separately or in combination with 1-hexano

238 Binding of DiC(6)SNPC with 2.0 mM Triton X-100 showed positive cooperativity (Hill coeffic

239 c(1) is fully active; however, protein-bound Triton X-100 significantly interferes with structural st

240 Triton X-100 solubility assays demonstrate that mutant D

241 First, we did cold Triton X-100 solubility assays.

242 ve compound, did not significantly alter the Triton X-100 solubility properties of the membrane.

243 both by fluorescence microscopy and by actin Triton X-100 solubility.

244 anol were microdissected and subjected to 1% Triton X-100 solubilization at 4 degrees C.

245 -48 h) certain mixtures were not amenable to Triton X-100 solubilization at one or more temperatures.

246 ne sphingolipids, for enabling resistance to Triton X-100 solubilization, and for interacting with sp

247 rol are colocalized and are resistant to the Triton X-100 solubilization.

248 amounts of soluble CD44S in POAG aqueous and Triton X-100-solubilized CD44H characterized POAG in the

249 Sucrose gradient analysis of Triton X-100-solubilized connexins showed that either Cx

250 uced using nonspecific alkaline phosphatase, Triton X-100-solubilized membranes from DGK1-overexpress

251 Cytochrome bc(1) isolated from Triton X-100-solubilized mitochondrial membranes contain

252 Using proteoliposomes reconstituted from Triton X-100-solubilized rat liver ER membrane proteins,

253 osomes prepared from phosphatidylcholine and Triton X-100-solubilized rat liver ER-membrane proteins.

254 minally truncated HMW2 derivative was partly Triton X-100 soluble and incapable of restoring HMW1, HM

255 TJ proteins were redistributed/localized in Triton X-100-soluble as well as Triton X-100-insoluble c

256 ereas total cellular EGFR was present in the Triton X-100-soluble fraction.

257 Dimers and oligomers were found in Triton X-100-soluble fractions from adenovirus-mediated

258 hyperphosphorylated occludin in lipid rafts, Triton X-100-soluble fractions, and the Triton X-100-ins

259 Claudin-4 was localized to Triton X-100-soluble gradient fractions of control or CP

260 in normal astrocytes exists primarily in the Triton X-100-soluble membrane fraction, distinct from th

261 Kv2.1 from the Triton X-100-insoluble to the Triton X-100-soluble membrane fraction.

262 raction of cell lysates and decreased in the Triton X-100-soluble pool.

263 Ubr1 function leads slGFP to accumulate in a Triton X-100-soluble state with slGFP degradation interm

264 quantitatively removed using injection of 5% Triton X-100 solution, generating a fresh surface for ea

265 ively removed using a cleaning method of 0.5%Triton X-100 sonication plus 1 N nitric acid sonication.

266 e inner membrane fraction was solubilized by Triton X-100, suggesting that GerD is a lipoprotein, and

267 he DMPC lipid membrane (tuned by addition of Triton X-100 surfactant or by the increase of the soluti

268 Interestingly, CHAPS and Triton X-100, thanks to similar surface binding preferen

269 ional channels, even without the presence of Triton X-100 that has been found necessary for in vitro-

270 When extracted with Triton X-100, the isolated Ndi1 did not contain Q.

271 Some tissues were exposed to Triton X-100 to establish dead tissue controls.

272 ndorff-perfused mouse hearts were treated by triton X-100 to produce endothelial dysfunction and subs

273 can be stimulated 35-fold by the addition of Triton X-100 to the reaction mixture.

274 function during reperfusion was impaired in triton X-100 treated hearts compared with vehicle-treate

275 y by affinity isolation using native but not Triton X-100-treated budded vesicles.

276 st, synaptic tau was partially soluble after Triton X-100 treatment and most likely represents aggreg

277 Triton X-100 treatment of transgenic mouse cardiac myofi

278 erently on native gels both before and after Triton X-100 treatment.

279 l sulfate (SDS), sodium deoxycholate, Chaps, Triton X-100, Triton X-114, NP-40, Brij-35, octyl glucos

280 llows: n-octyl glucoside, dodecyl maltoside, Triton X-100, Tween 20, 3-[(3-cholamidopropyl)dimethylam

281 xperiments in which neutral additives (e.g., Triton X-100, Tween 20, poly(ethylene glycol)) are remov

282 I) and ABI-attenuation by additives, such as Triton X-100 (TX) and human serum albumin (HSA), are not

283 ort, we confirm that TRAF2 translocates to a Triton X-100 (TX)-insoluble compartment upon TNF-R2 enga

284 month-old mice by intranasal irrigation with Triton X-100 (TX).

285 by using extraction with the mild detergent Triton X-100 (TX-100) followed by a sucrose gradient flo

286 (OLs) in culture MOG is not raft associated [Triton X-100 (TX-100) soluble, 4 degrees C].

287 rlin resists solubilization by the detergent Triton X-100 (TX-100), a property commonly attributed to

288 majority of CEACAMs were solubilized by cold Triton X-100 (TX-100), indicating their absence from lip

289 s the tegument was resistant to removal with Triton X-100 (TX-100), whereas it was lost nearly comple

290 e demonstrated that Gs alpha migrates from a Triton X-100 (TX-100)-insoluble membrane domain (lipid r

291 otein expression by Western blot analysis of Triton X-100 (TX-100)-soluble and TX-100-insoluble cell

292 (erythro-GUVs) when exposed to the detergent Triton X-100 (TX-100).

293 ity, and viability caused by the presence of Triton X-100 (TX100), a nonionic surfactant, were studie

294 exhibit differential functional stability in Triton X-100 versus dodecyl maltoside.

295 Sucrose gradient analysis of Triton X-100 virus lysates showed that Gag and envelope

296 In one assay, insolubility in Triton X-100 was assessed in bilayers composed of sterol

297 s of IPC synthase activity solubilized in 2% Triton X-100 was between 1.5 x 10(6) and 20 x 10(6) Da,

298 An average LC50 value of 138 microM for Triton X-100 was obtained for an incubation period of 7-

299 from beta(2)m upon exposure to the detergent Triton X-100, whereas a mutant expressing only glycan 2

300 Combining the use of Triton X-100 with the newly introduced UPLC method, we w