ライフサイエンスコーパス: V region

1 ited by antibodies directed against the Scl1-V region.

2 ocuses pre-BCR-dependent selection on the HC V region.

3 vity to multiple residues located across the V region.

4 start at CDR3 position four, well within the V region.

5 ne contained a G2576U mutation in the domain V region.

6 V mutation in vivo and its targeting to the V region.

7 re efficient AID deamination target than the V region.

8 d motifs and, to some degree, throughout the V region.

9 f its BCR to an idiotypic epitope in its own V region.

10 grafted into a nonhomologous human germ line V region.

11 iversity may be generated by mutation in the V regions.

12 antibody responses against a wide variety of V regions.

13 ed in combination with deletions of selected V regions.

14 ntral and peripheral T cell tolerance to BCR V regions.

15 ited cross-reactivity with heterologous TprK V regions.

16 sponse during infection is directed to these V regions.

17 paired somatic hypermutation (SHM) of the Ig V regions.

18 y specificity is a function of the variable (V) region.

19 ir generates mutations within immunoglobulin V-regions.

20 rgoes antigenic variation in seven variable (V) regions.

21 1 are found at "active" (DJ) and "inactive" (V) regions.

22 ty of antibodies sharing identical variable (V) regions.

23 n of different Env proteins, suggesting anti-V regions 1 and 2 Abs may be a marker of ADCC breadth.

24 FcgammaR-binding Abs specific to V regions 1 and 2 were strongly associated with increase

25 eterminants of Env, the CD4 binding site and V region 3, could in part account for the neutralizing a

26 itory receptor genes that encode a variable (V) region, a unique V-like C2 (V/C2) domain, a transmemb

27 r reactivity to patients' sera carrying anti-V region Abs to COL-1.

28 Looping of a subset of IgH V regions, albeit at lower frequency, was also observed

29 immune repertoires including the entire IgH V region and enough of the IgH C region to identify isot

30 ay is regulated by the alternatively spliced V region and high-affinity heparin-binding domain of fib

31 ls, which depends on the location within the V region and is isotype and subclass dependent.

32 w antisense transcription is specific to the V region and suggest transcripts extend across several g

33 s the CS-1 site on the alternatively spliced V region and the EIIIA region.

34 Idiotypes (Ids) are unique epitopes of Ab V regions and can trigger anti-Id immune responses, but

35 This interchangeability of TCR V regions and CDR3 motifs permits multiple structural so

36 vs, including gp160 or gp145 with or without V regions and gp41 deletions.

37 TCR beta- and alpha-chains showed identical V regions and invariant charged residues within the CDR3

38 of physical specificity between TCR germline V regions and MHC.

39 sively contained somatically mutated lambda1 V regions and were capable of producing Ag-specific Ab-f

40 The mutations were limited to V regions and were localized in known hotspots.

41 d in part by its syndecan-specific variable (V) region and in part by the second conserved (C2) regio

42 volved are specific to families of variable (V) regions and to some extent different rules may govern

43 s that the local sequence environment of the V region, and especially of the CDR1 and CDR2, is highly

44 BCRs, insufficient adaptive mutations in Ab V regions, and conformational instability of gp120.

45 eterogeneity is localized to seven variable (V) regions, and tprK sequence diversity accumulates with

46 ame is localized in seven discrete variable (V) regions, and variability results from apparent base c

47 he exact profile of rearrangement within the V region appears to be V gene specific.

48 These V regions are identical to those recently found in a reg

49 The TprK V regions are the focus of anti-TprK antibodies arising

50 These V regions are the targets of the host humoral immune res

51 body and have deimmunized both the variable (V) regions as well as the junction between the heavy (H)

52 athway by which microbial toxins that target V region-associated BCR sites induce programmed cell dea

53 ranslocations, and share features typical of V-region-associated somatic hypermutation.

54 tructural constraints imposed by C region on V region binding.

55 paring codons of CDR and FW of the germ-line V regions both to each other and to control regions.

56 ally matured versions of a type II variable (V) region, both in the presence and absence of its antig

57 of the different genes encodes an N-terminal V region but differs in the number of extracellular Ig d

58 dogenes are not evenly spread throughout the V region, but rather cluster together.

59 ucing mutations templated by adjacent pseudo-V regions, but impairment of gene conversion switches mu

60 e to MHC binding by other TCRs using related V regions, but not usually so dominantly.

61 ving exceptionally long CDR H3 loops and few V regions, but the mechanism for creating diversity is n

62 contrast, D47 and D19 IgG, sharing the same V regions, but which were not transcytosed by the pIgR,

63 and we propose a model for generation of new V regions by segmental gene conversion.

64 New sequences were observed in all seven V regions by the fifth slow passage.

65 These data show that one TCR V region can mediate a mechanism of recognition of two r

66 that frequently result in the attachment of V region carbohydrate.

67 four criteria--the activities of N-cadherin/V region chimeras, syndecan-1 deletion mutants, or synde

68 the immunoglobulin heavy-chain variable (Igh-V) region compared with the constant region and partiall

69 t immunogen binding to Abs in their germline V region configuration expressed as BCRs, insufficient a

70 stricting flexibility, maintains a favorable V region conformation to allow superagonistic activity.

71 ontacts compared with wild-type, yet the TCR V-region contacts with MHC are conserved.

72 In contrast to switch regions, V regions contained predominantly single-strand breaks,

73 V region-containing chitin-binding proteins (VCBPs) cons

74 V region-containing immune-type genes constitute a far m

75 s of mouse-human chimeric Abs with identical V regions demonstrate differences in fine specificity an

76 These activities correlate with a V-region-dependent incorporation of cell-surface syndeca

77 B cells can present V region-derived Id peptides on their MHC class II molec

78 V region-derived idiotypic (Id) peptides can be displaye

79 and 3, both germline and somatically matured V regions displayed significant structural changes in th

80 Chicago strain of T. pallidum and confirmed V region diversification during passage of this isolate.

81 V region diversity, particularly in V6, accumulates more

82 istinct B cell subpopulation with limited Ig V region diversity.

83 Abs can acquire N-linked glycans in their V regions during Ag-specific B cell responses.

84 ucleotide long RNA, comprising loop A, helix V, region E and helix IV, but lacking helix II, retains

85 The demonstration that the V regions elicit a variant-specific antibody response su

86 as diverse, with five of six MAb heavy-chain V regions encoded by distinct members of the J558 family

87 mutated heavy chain immunoglobulin variable (V) region encoded by IGHV1-69, IGHD3-16, and IGHJ3 with

88 Focused libraries are built using V regions encoding combinations of canonical structures

89 ion, antibodies developed against all of the V regions except V1 and V3.

90 one, antibodies developed against all of the V regions except V1, while antibodies developed against

91 us, multigenic families encoding diversified V regions exist in a species lacking an adaptive immune

92 n the primary structure of the H and L chain V regions exist, the possibility that this level of rest

93 cells generated via preassembled IgH and IgK V region exons (HL).

94 assembly and subsequent selection of TCRbeta V region exons during thymocyte development.

95 Combinatorial assembly of IgH and IgL V region exons from gene segments generates preimmune Ig

96 utations that arose in the IGHV3-23*01 human V region expressed in vivo by human memory B cells revea

97 e initial immune response raised against the V regions expressed in the inoculum.

98 In summary, V-region expression in the context of the constant (C) r

99 Clonal evolution of Ig V regions, expression of activation-induced cytidine dea

100 se includes assignments to the IMGT germline V regions for heavy and light chains for several species

101 The average clone had 37 mutations in the V region, for a frequency of 0.11 mutations/base.

102 erantigens, which can interact via conserved V region framework subdomains of the Ag receptors of lym

103 gion heavy chain (V(H)) clan III genes via a V region framework surface that has been highly conserve

104 transcription cofactor Spt5 and AID, in the V region from germinal center cells, which is totally ab

105 ructed by domain swapping, which contain the V region from Scl1 proteins, were able to bind to ApoB10

106 generated phage-displayed libraries of IgNAR V regions from an immunized animal and found a family of

107 Sequencing of V regions from CD138(+) cells in MS CeSF has revealed so

108 nst synthetic peptides representing the TprK V regions from each clone.

109 ulin heavy chain (IgH) locus is critical for V region gene assembly.

110 TCR beta-chain V region gene diversity was determined by sequencing.

111 Our results reveal a subtle tier of V region gene evolution in which DNA sequence has been m

112 ssion through analysis of the TCR beta-chain V region gene products expressed in samples obtained fro

113 se collection of heavy-chain and light-chain V region gene products to form specific paratopes, with

114 Secondary Ab V region gene segment rearrangement, termed receptor edi

115 TRBV2 (formerly BV22) were the most expanded V region gene segments in DR3(+) LS patients relative to

116 arising from the use of common H and L chain V region gene segments that share CDR3 structural featur

117 An immune network that has limited V region gene usage likely exists in the CSF and central

118 We studied the V region gene use in murine anti-MPO Abs derived from Sp

119 The variable (V) region gene encoding the heavy (H) chain underwent V-

120 This enables a single variable (V) region gene to be used in conjunction with different

121 Variable (V) region gene usage was diverse, with five of six MAb h

122 We sequenced Ab H and L chain V region genes (V(H) and V(L)) of clones expanded from s

123 study the selection of individual Ab H chain V region genes (V(H)), we performed CDR3 spectratyping o

124 th aberrant AID expression in Ph(+) ALL, IGH V region genes and BCL6 were mutated in many Ph(+) but u

125 Sequences of these V region genes demonstrated that each hybridoma expresse

126 led to productively rearrange immunoglobulin V region genes encoding a functional B-cell receptor (BC

127 studying nonproductive (hence unselected) Ig V region genes for somatic mutations and processed pseud

128 finding that the framework 2 region of kappa V region genes is highly mutable despite its importance

129 Analysis of the rearranged H chain V region genes of mAbs isolated from seven of these wome

130 The TCR gamma translocon contains at least 5 V region genes, 3 J segment genes, and 1 C segment.

131 crease occurring in cases with unmutated IgH V region genes.

132 ll development, the immunoglobulin variable (V) region genes are subject to somatic hypermutation in

133 Ig variable (V) region genes are subjected to a somatic hypermutation

134 bstitutions into immunoglobulin variable (Ig V) region genes at all four bases, but the mutations at

135 d immunoglobulin heavy chain (IGH) variable (V) region genes devoid of somatic mutations.

136 atic mutation of immunoglobulin variable (Ig V) region genes is an important prognostic indicator of

137 Somatic mutation in immunoglobulin variable (V) region genes occurs largely in the germinal center an

138 of heavy and light chain germ line variable (V) region genes to form pneumococcal capsular PS (PPS) 6

139 the rearranged immunoglobulin (Ig) variable (V) region genes, and class-switch recombination (CSR) al

140 ypermutation (SHM) of the antibody variable (V)-region genes.

141 dominantly immunoglobulin G, were encoded by V-region genes expressed late in development, and displa

142 ng clonal relatives or by usage of different V-region genes.

143 Basic knowledge about patterns of V region glycosylation at different stages of B cell dev

144 These results suggest that inappropriate V region glycosylation could contribute to ineffective A

145 nce analysis of B-1a, B-1b, and B-2 cell IgH V region H chain (V(H)) genes revealed increased usage o

146 ow passages (30- to 35-day intervals), three V regions had sequences that were completely different f

147 The BCR V region has been implicated as a potential avenue of T

148 cytidine deaminase targets the DNA encoding V regions, has enabled the analysis of its targeting pro

149 portions but with minor differences in their V regions have been demonstrated to interact with FcRn w

150 fold above normal, are targeted primarily at V-region hot spots by unknown mechanisms.

151 In contrast to mutations in V regions, however, these mutations are not detectable i

152 onse, B cells undergo Ab class switching and V region hypermutation, with the latter process potentia

153 more, isotype affected the polyreactivity of V region identical antibodies, implying a role for C reg

154 (V) region to affect paratope structure in a V region identical IgG(1), IgG(2a), IgG(2b), and IgG(3)

155 is study we compared the fine specificity of V region-identical IgE and IgA to Cryptococcus neoforman

156 leave the peptide at a much faster rate than V region-identical IgG subclasses and IgA, consistent wi

157 namic properties of the interactions of four V region-identical monoclonal antibodies with a univalen

158 hypermutation (ac-Nglycs) within Ig H chain V region (IGHV) genes as alternative selective pressures

159 using high-throughput sequencing of the IgH V region (IGHV) genes, we have studied features of TG2-s

160 The IgH V region (IGHV) is central to Ag binding and consists of

161 d-type cells, substitution of a heterologous V region impaired the survival of three ABC lines.

162 o demonstrate functional roles of syndecan-1 V region in laminin-dependent C2C12 cell adhesion and th

163 eptides derived from the immunoglobulin (Ig) V region in some but not all patients.

164 animal indicating the functionality of this V region in the context of immune defense against pathog

165 demonstrate the dominant role of the H chain V region in TSHR recognition.

166 found that RNA polymerase II accumulates in V regions in B cells after both types of stimulation for

167 The usage of TCR alpha and beta V regions in the cell line was oligoclonal.

168 e the possibility that expression of certain V regions in the context of alpha and C regions affects

169 ue to the failure to utilize the appropriate V regions in the pre-immune repertoire.

170 be a global defect in T cell tolerance to Ab V regions in this autoimmune-prone strain either before

171 his study, we searched for ssDNA in mutating V regions in which DNA-protein complexes were preserved

172 K (TprK) differs in seven discrete variable (V) regions in isolates and that the antibody response du

173 LE in which all somatic mutations within ANA V regions, including those in CDR3, could be unequivocal

174 pecificity is solely the result of variable (V)-region interactions with an antigen.

175 However, monoallelic looping of IgH V regions into close proximity of the IgH DJ cluster was

176 eable TAT-V peptide--we demonstrate that the V region is necessary and sufficient for these cell beha

177 tained when a peptide from the BCR variable (V) region is expressed by small numbers of B cells as it

178 w the somatic mutation of antibody variable (V) regions is generated.

179 We confirmed these findings in an analogous V region knock-in mouse and/or in non-Tg mice.

180 a cells secrete a monoclonal Ig containing a V region L chain (VL) epitope recognized by CD4+ T cells

181 CD8(+) T cells recognized overlapping third V region loop peptides.

182 er that innate responses of antigen receptor V-regions may be more widespread, for example, inducing

183 rface Ig is mandatory and carries a striking V-region modification because of introduction of glycan

184 The results suggest that V-region motifs associated with annular binding and opso

185 V region mutation hotspots are largely determined by AID

186 allenging assumptions about the location and V region mutation status of memory cells.

187 sites of B cell responses, and they include V-region mutation and result in liver MBC localization.

188 s exhibit 5-10-fold increases in heavy-chain V-region mutations targeted only predominantly to RGYW (

189 ss the transcription factor T-bet and harbor V-region mutations.

190 shows selection for rearrangement within the V region of a number of genes and for CD8 and CD4 cells.

191 ccurring somatic mutations in the Ab H chain V region of Fab19, a well-described neutralizing human m

192 Somatic hypermutation (SHM) diversifies the V region of Ig genes and underlies the process of affini

193 ed carbohydrates are frequently found in the V region of Ig H chains and can have a positive or negat

194 dependence of the kinetics revealed that the V region of IgG could modulate a degree of conformationa

195 e have tested the hypothesis that the unique V region of syndecan-1 cytoplasmic domain has a crucial

196 haviors that depend on signaling through the V region of syndecan-1.

197 nce is caused by mutations within the domain V region of the 23S ribosomal RNA (rRNA) gene, which is

198 A 420-bp domain V region of the 23S rRNA gene from all isolates was ampl

199 presence of a T2500A mutation in the domain V region of the 23S rRNA gene.

200 act to immunogenic peptides derived from the V region of the BCR that are created by somatic mechanis

201 ation-induced cytidine deaminase mutates the V region of the Ig genes to increase the affinity of Abs

202 ic and intergenic, occurs extensively in the V region of the immunoglobulin heavy chain locus.

203 Furthermore, the non-collagenous V region of the Scl1 protein is responsible for LDL/ApoB

204 that differed solely with respect to the IgH V region of their BCRs.

205 oo contain an amino-terminal non-collagenous V region of unknown function.

206 rated a double knock-in mouse that expresses V regions of a somatically mutated anti-Id mAb with inte

207 teins that interact with conserved motifs in V regions of B cell Ag receptor shared by large sets of

208 Analysis of somatic mutations in V regions of Ig genes is important for understanding var

209 quences that are preferentially found in the V regions of Ig genes.

210 tion of a human rIgA(2) mAb that carries the V regions of IgG1 b12, a potent and broadly neutralizing

211 lish patterns of N-glycosylation sites in Ab V regions of naive and memory B cell subsets.

212 e demonstrate that antibody responses to the V regions of one TprK molecule show limited cross-reacti

213 quisition of N-glycosylation sites within Ab V regions of peripheral blood and bone marrow B cells of

214 dues within the basic pocket and surrounding V regions of the B30.2 domain abrogated prenyl pyrophosp

215 Antibodies were highly specific for the V regions of the infecting clone, and cross-reactivity w

216 e describe the sequence anatomy of the seven V regions of tprK and the identification of putative don

217 for targeting AID activity to the variable (V) region of Ig genes are unknown.

218 The genes encoding the variable (V) region of the B-cell antigen receptor (BCR) are assem

219 However, the Variable (V) regions of antigen receptors include germ line-encode

220 of somatic hypermutation (SHM) of variable (V) regions of Ig genes.

221 Instead, the variable (V) regions of IgNAR bind antigen as soluble single domai

222 genomic DNA sequencing of multiple variable (V) regions of the bacterial 16S ribosomal gene, to inter

223 the possibility that looping of distinct IgH V regions plays a role in promoting long-range interacti

224 known about their genetics or the variable (V) region polymorphisms that affect their protective fun

225 Antibodies to the N-region or V-region polypeptides, but not antibodies to the rC-Msp

226 The N- and V-region polypeptides, but not rC-Msp, also bound to the

227 We determined complete V region primary structures and performed antigen bindin

228 advantage of in vivo affinity-maturation of V regions prior to library generation.

229 -regulates Ig H chain production from select V region promoters and requires Bright dimerization, Bru

230 he results suggest that the C region affects V region protein conformation, leading to differences in

231 developed CLL clones with identical antibody V regions randomly is highly improbable and suggests sel

232 ydrate addition sequence generated by either V region rearrangement or somatic hypermutation may resu

233 traightforward, even when the same variable (V) region recognizes the same MHC molecule.

234 hat the diversity of the expressed variable (V) region repertoire of the immunoglobulin (Ig)H chain o

235 e ability of TCR chain pairing to modify how V region residues interact with pMHC helps to explain ho

236 to bind to its own cell surface depended on V region residues that mediate the intrinsic autoreactiv

237 To minimize anti-V region responses, a variant of HuCOL-1 was generated b

238 The V region-restricted histone hyperacetylation resulting f

239 Targeting of AID to antibody variable (V) regions results in somatic hypermutation, whereas its

240 rrangement frequency distribution within the V region reveals selection on CDR3 position four.

241 Light-chain V-region segments were derived from the Vk1, Vk4/5, Vk10

242 d passages (9- to 10-day intervals), no tprK V region sequence changes were observed.

243 assembled the entire 2.5-Mb mouse IgH (Igh) V region sequence of the C57BL/6 strain from public sequ

244 ective efficacy, presumably due to variable (V)-region sequence differences resulting from somatic mu

245 ted for approximately 70% of the IgG H chain V regions sequenced, while the corresponding IgM reperto

246 ammadelta cells and demonstrating that Vbeta V region sequences are not required for selection of an

247 This indicates that gene conversion between V region sequences can occur in mouse B cells; we propos

248 To test our hypothesis that V region sequences change during infection and passage,

249 determine the distribution of TCR beta-chain V region sequences expressed in the transferred cells as

250 Sequence analysis of amplified IgG V region sequences identified the rearranged germline se

251 abbits may explain the limited repertoire of V region sequences seen in the Nichols strain.

252 The parent strain expresses many different V region sequences, and infection with this strain induc

253 entification of putative donor sites for new V region sequences, and we propose a model for generatio

254 rance in developing T cells specific for BCR V region sequences, thus ensuring that T cell help to B

255 ivation-induced cytidine deaminase acting on V-region sequences is sufficient to initiate authentic f

256 Somatic mutations in the IgNAR V region serve to increase the number of contacts with a

257 Sequence analysis of antibody V regions showed evidence of somatic and affinity matura

258 microbial Ig-binding protein specific for a V region site on Ig L chains.

259 oducing mutations within the antigen-binding V regions (somatic hypermutation, SHM) and double-strand

260 terodimeric configurations with other native V-region splice variants in cartilage.

261 es not efficiently heterodimerize with other V-region splice variants of fibronectin.

262 We conclude that the C region can modify the V region structure to alter the Ab paratope, thus provid

263 tributed the major variability in the intact V region structure.

264 affect binding affinity and specificity and V-region structure.

265 ient B cells were mostly specific for the Ig V region, suggesting a local or time-dependent need for

266 (A) and proline-rich (P) repeats flanking a V region that is projected distal from the cell.

267 However, breaks in V regions that arise during somatic hypermutation are po

268 We found that V regions that undergo SHM were enriched in short patche

269 ceptors (BCRs) for antigen express variable (V) regions that are enormously diverse, thus serving as

270 somatic hypermutation (SHM) of Ig variable (V) regions that is required for the affinity maturation

271 -X-serine/threonine (N-gly sites) within the V-region that are rarely found in normal B-cell counterp

272 ass switch serves to distribute a particular V region to different Ig C regions.

273 se that antisense transcription remodels the V region to facilitate V(H)-to-DJ(H) recombination.

274 conformational constraints on the variable (V) region to affect paratope structure in a V region ide

275 in (IgH) gene that allows the same variable (V) region to be expressed with any one of the downstream

276 CRs had an alpha-chain bearing the variable (V) region TRAV1-2 rearranged to the joining (J) region T

277 ne profiling and deep sequencing of TCR-beta V regions, two subsets of cTregs, based on expression of

278 Rearranged Ig V regions undergo activation-induced cytidine deaminase

279 Secondary diversification occurs when Ig V regions undergo somatic hypermutation (SHM) and affini

280 sm responsible for the formation of ssDNA in V regions undergoing SHM has yet to be experimentally es

281 treatment with a TLR9 agonist protected IgH V region unmutated, but not mutated, CLL cells from apop

282 blood, in the case of B-CLL with Ig H chain V region-unmutated BCR and <30% CD38(+) cells in blood.

283 murine IgG switch variants that differed in V region usage for Cryptococcus neoformans glucuronoxylo

284 IgH V regions used by the BCRs of ABC DLBCL biopsy samples v

285 adults comparing Spanish regions using PCV13 vs regions using PPV23 vaccine was also analyzed for 201

286 cted mutagenesis of the MAb 12A1 heavy-chain V region (V(H)) was followed by serological and function

287 CSR) by deaminating cytidines to uridines at V region (V) genes and switch (S) regions.

288 V neutralizing murine IgM with H and L chain V regions (V(H) and V(L) regions) free of immunogen-driv

289 is, the TprK Ag undergoes variation of seven V regions (V1-V7) by nonreciprocal recombination of sile

290 "semi-composite" 16S profiles using multiple V regions validated by quantitative PCR analysis confirm

291 oxymethyl that forms a common TCR beta-chain V region variant.

292 ally done by interrogation of paired H chain V region (VH) and L chain V region (VL) sequences of ind

293 of paired H chain V region (VH) and L chain V region (VL) sequences of individual and Ag-specific B

294 n, the frequency of mutations throughout the V region was reduced.

295 n reported to be associated with light chain V regions, we have begun an analysis of germ line light

296 this, we found that both DNA strands in the V region were transcribed.

297 Expressed Ig V regions were amplified by RT-PCR.

298 ete and definitive assembly of the mouse Igh V region will facilitate detailed study of promoter func

299 e C region can affect the interaction of the V region with an Ag.

300 somatic hypermutation of rearranged lambda1 V regions within secondary AFCs showed a strong correlat