ライフサイエンスコーパス: WGA

1 WGA allows amplification of the entire genome, which gre

2 WGA binding affinity can be further improved (K(D) ~ 10

3 WGA bound exclusively to SA residues on the apical surfa

4 WGA cells represent a powerful system to study the regul

5 WGA samples have high call rates (97.5% on average, comp

6 WGA was demonstrated to have high specificity for Lp(a)

7 WGA, MAA, and concanavalin A significantly inhibited fer

8 WGA-Fc directly inhibited fungal growth in standard cult

9 WGA-Fc opsonization increased fungal phagocytosis, as we

10 WGA-HRP injections in conjunction with GAD immunohistoch

11 WGA-X enhances genome recovery from individual microbial

12 Following this strategy, 20 WGA products from six Cryptosporidium species or genotyp

13 wever, showed significant contamination in 5 WGA products (proportion of positive colonies derived fr

14 Fifty nanoliters of 2.5% WGA-HRP were microinjected into the NMC in the cat.

15 nterpret the set of P-values emerging from a WGA study.

16 label in these same regions as a result of a WGA-HRP injection suggests that the connections are reci

17 involves binding of synthesized chitin to a WGA-coated surface followed by detection of the polymer

18 ear the base of the heart of muskrats with a WGA-HRP solution to label retrogradely preganglionic par

19 vercomes the current limitations of accurate WGA, which is the major obstacle to studying genetic div

20 In addition, WGA-HRP labeling was occasionally observed in lamina I.

21 ing sites is ca. 9 A, wheat germ agglutinin (WGA) (shortest distance between binding sites 13-14 A),

22 luorophore-conjugated Wheat Germ Agglutinin (WGA) and a lipophilic blue fluorochrome, Ac-201, for the

23 ther, coexpression of wheat germ agglutinin (WGA) and an axon-targeted beta-gal supports mapping both

24 ciated virus encoding wheat germ agglutinin (WGA) and by immunoelectron microscopy determined the pre

25 ere we use the lectin wheat germ agglutinin (WGA) as ligand; WGA inhibits nuclear transport and has b

26 Wheat germ agglutinin (WGA) binds to the glycosylated extracellular domain III

27 Wheat germ agglutinin (WGA) binds with high affinity and specificity to several

28 Wheat germ agglutinin (WGA) bound only to the outermost region of J3 and produc

29 l PM by absorption on wheat germ agglutinin (WGA) did not deplete G alpha subunits.

30 ble to HRP-conjugated wheat germ agglutinin (WGA) disrupted delivery of HA but not endolyn.

31 st walls (PCWs) or to wheat germ agglutinin (WGA) inhibited excystation by > 90%.

32 romatography and by a wheat germ agglutinin (WGA) lectin affinity column.

33 Wheat germ agglutinin (WGA) reactive glycans on fibronectin and thrombospondin-

34 Wheat germ agglutinin (WGA) showed significantly decreased binding to adenomas

35 normal phalloidin and wheat germ agglutinin (WGA) staining of Tdrd7-/- fiber cells, particularly thos

36 neuronal tract tracer wheat germ agglutinin (WGA) to nonpeptidergic nociceptive neurons.

37 anavalin A (ConA) and wheat germ agglutinin (WGA) to their target monosaccharides indicating affinity

38 gent against mycosis, wheat germ agglutinin (WGA) was linked to the effector Fc region of murine IgG2

39 ion protocol based on wheat germ agglutinin (WGA), a lectin that binds to N-acetylglucosamine and sia

40 s (K(D) ~ 40 nM) with wheat germ agglutinin (WGA), a model lectin that exhibits multivalent binding w

41 transneuronal tracer, wheat germ agglutinin (WGA), in the 5HT neurons so as to study the interplay be

42 transneuronal tracer, wheat germ agglutinin (WGA), is induced in primary sensory neurons, but only af

43 and the more general wheat germ agglutinin (WGA), were selected to label REVs.

44 ed-conjugated lectin, wheat germ agglutinin (WGA), which binds SA residues.

45 by Co2+, followed by wheat germ agglutinin (WGA)-affinity HPLC or size-exclusion HPLC (SE-HPLC) usin

46 The retrograde tracer wheat germ agglutinin (WGA)-apoHRP-gold was used to identify neurons with appro

47 eveloped by employing wheat germ agglutinin (WGA)-coated Flashplates.

48 By using wheat germ agglutinin (WGA)-coated scintillation proximity assay (SPA) beads to

49 ately 50% decrease in wheat germ agglutinin (WGA)-labeled components of the GCX under DF conditions,

50 ), jacalin (JAC), and wheat germ agglutinin (WGA).

51 avalin A (Con A), and wheat germ agglutinin (WGA).

52 ydrate affinity using wheat germ agglutinin (WGA).

53 blocked by the lectin wheat germ agglutinin (WGA).

54 ession of the lectin, wheat germ agglutinin (WGA).

55 cell-binding ligand, wheat germ agglutinin (WGA).

56 ivity with the lectin wheat germ agglutinin (WGA).

57 oxidase conjugated to wheat germ agglutinin (WGA-HRP) with biotinylated dextran amine (BDA) transport

58 can-binding domain of wheat germ agglutinin (WGA-HRP).

59 The lectin, wheatgerm agglutinin (WGA) conjugated to horseradish peroxidase (HRP), previou

60 e general problem of whole-genome alignment (WGA).

61 t_pangenes performs whole genome alignments (WGA) to call syntenic gene models based on coordinate ov

62 Further, whole-genome amplification (WGA) amplified contents from a few retrieved cells, foll

63 was processed by whole genome amplification (WGA) and hybridized onto a D. mccartyi microarray for co

64 chnologies using whole genome amplification (WGA) and preimplantation genetic haplotyping (PGH) of em

65 rcalator dye and whole genome amplification (WGA) assays.

66 ping methods for whole genome amplification (WGA) by random or degenerate oligonucleotide-primed PCR.

67 me this is to do whole-genome amplification (WGA) in clinical samples, but only limited studies have

68 Whole-genome amplification (WGA) is a useful tool for amplification of very small qu

69 f the GenomePlex whole genome amplification (WGA) kit to amplify frozen and FFPE tissue for use in ar

70 ts introduced in whole-genome amplification (WGA) make it difficult to derive accurate genomic inform

71 However, current whole-genome amplification (WGA) methods are limited by low accuracy of copy-number

72 Whole-genome amplification (WGA) methods offer a solution for this problem, and earl

73 cation steps and whole-genome amplification (WGA) of DNA from purified oocysts.

74 NA obtained from whole genome amplification (WGA) of RNA and DNA from sewage and animal (avian, cattl

75 Whole genome amplification (WGA) of single cells is generally the first step in such

76 on sequencing, a whole-genome amplification (WGA) process is required, but it substantially distorts

77 ection (LCM) and whole-genome amplification (WGA) to separately collect and amplify DNA from adjacent

78 vation and novel whole-genome amplification (WGA) to sequencing platforms and genome assembly.

79 ally single cell whole genome amplification (WGA) was employed to pre-amplify the single-cell DNA by

80 Improvements in whole genome amplification (WGA) would enable new types of basic and applied biomedi

81 ein analysis and whole genome amplification (WGA), and we demonstrate this by doing both for the same

82 ailable kits for Whole Genome Amplification (WGA), PicoPLEX and RepliG were compared on small amounts

83 cell sorting and whole-genome amplification (WGA), we are able to generate high-quality material from

84 cation (MDA) for whole-genome amplification (WGA), which is followed by high-throughput sequencing.

85 have developed a whole genome amplification (WGA)-based genotyping assay for PKD1 and PKD2, and exami

86 procedure before whole genome amplification (WGA).

87 specific PCR and whole genome amplification (WGA).

88 nting the use of whole-genome amplification (WGA).

89 DNA content and whole-genome amplification (WGA); this is followed by next-generation sequencing to

90 Weak interactions with MAL, Con A, and WGA were also quantified.

91 Subsequent application of the FACS and WGA protocols to two enrichment cultures containing appr

92 A, based on primase-polymerase features, and WGA-X employing a thermostable phi29 polymerase, were pr

93 A significantly inhibited fertilization and WGA was found to block fertilization by preventing sperm

94 LM-PCR and WGA, the most popular sample amplification techniques, r

95 Exposure of cysts to anti-PCW and WGA prior to, but not after, stage II was sufficient to

96 n kidney biopsies stained with SYTO16/PI and WGA.

97 ar antigen, which is composed of protein and WGA reactive carbohydrate, and indicate that cross-react

98 from the Q-Sepharose, heparin-Sepharose, and WGA-agarose also indicated only the single radiolabeled

99 raphy on Q-Sepharose, heparin-Sepharose, and WGA-agarose.

100 scently labeled WGA with a metal-tagged anti-WGA antibody, we introduce a dual-labeling strategy comp

101 We have further applied WGA to ADPKD mutation analysis of low DNA-yield specimen

102 at can impede de novo whole-genome assembly (WGA).

103 framework of post-whole genome association (WGA) annotation, we have developed WGAViewer, a suite of

104 were identified by whole genome association (WGA) mapping of cytokine concentrations.

105 MDA-based WGA is a simple and reliable method that could have sign

106 ast to 4-6 orders of magnitude for PCR-based WGA methods.

107 trograde labeling of trigeminal afferents by WGA injection into the tip of the tongue showed an incre

108 ated by anti-Hs immunoglobulin G and also by WGA.

109 Inhibition by WGA was reversed by either chitotriose or sialic acid, w

110 8 weeks, ocular dominance stripes labeled by WGA-HRP appeared adultlike with smooth, sharply defined

111 The area of cell bodies labeled by WGA-HRP appeared similar to the area of cell bodies labe

112 Here we report an improved single-cell WGA method, Linear Amplification via Transposon Insertio

113 wn to exceed previously reported single-cell WGA methods in amplification uniformity, genome coverage

114 t performance in high-throughput single-cell WGA remains elusive.

115 ected for further investigation by combining WGA analysis with previously published QTL for murine re

116 In conclusion, WGA-based LR-PCR represents a major technical improvemen

117 supporting nor globose basal cells contained WGA-HRP, suggesting that uptake was primarily into olfac

118 bution of NRA axons in the lumbosacral cord, WGA-HRP injections were made into the NRA in seven monke

119 ng phased parental genotypes and deciphering WGA-distorted SNP B-allele fractions via a process we co

120 lied for the first time a recently developed WGA method, multiple displacement amplification (MDA), t

121 t only limited studies have tested different WGA methods in FFPE cancer specimens using targeted next

122 These findings suggest that each WGA method should be tested thoroughly before using it f

123 Although numerous studies employing WGA have focused primarily on clinical applications, few

124 However, existing WGA methods like degenerate oligonucleotide-primed PCR s

125 As expected, WGA products with low (<16.0) threshold cycle (CT) value

126 Both approaches resulted in extensive WGA expression in the cell bodies and dendrites of neuro

127 The facile WGA-HRP method permitted high surface coverage of cellul

128 The evolving technology of PGH following WGA may increase the diagnostic scope and availability o

129 ed nucleus of the stria terminalis following WGA-Au-HRP injections that incorporated the rostrolatera

130 Triple labeling for WGA-Au, Fos, and orexin revealed that the percentage of

131 The tissue was processed sequentially for WGA-HRP, and then BDA immunohistochemistry using two dif

132 er aberrations have to be discriminated from WGA artifacts.

133 ding on the application, background DNA from WGA kits can be problematic.

134 ther than acoustic fragmentation to generate WGA-free whole-genome libraries.

135 d to horseradish peroxidase coupled to gold (WGA-Au-HRP) was injected into either the nucleus LC or t

136 al cord involved placement of the tracer HRP/WGA-HRP on the cut end of the nerve root.

137 Changes in WGA binding to the human surface epithelium allowed regi

138 pre-amplification is the only method used in WGA of single sperm, whereas it has limited capacity to

139 , some inter- and intrasample variability in WGA was observed, but these biases were minimized by per

140 bohydrates and a range of lectins, including WGA, Con A, UEA-I, GS-II, PNA and SBA, were monitored in

141 xylic acid or hydroxyl groups, do not induce WGA binding.

142 By integrating WGA-X with calibrated index-cell sorting and high-throug

143 -directed amplification (PTA), an isothermal WGA method that reproducibly captures >95% of the genome

144 peptide sequence was obtained from a 220-kDa WGA-binding protein.

145 It behaves as a 250 kDa, WGA-reactive glycoprotein with a basic isoelectric point

146 Double-labeled WGA-HRP/ChAT neurons were found in the pedunculopontine

147 Double-labeled WGA-HRP/NADPH-d-positive neurons could be seen in many n

148 By combining fluorescently labeled WGA with a metal-tagged anti-WGA antibody, we introduce

149 llustrate the diverse uses of these ZW (LacZ-WGA) mice, we triggered WGA expression either by crossin

150 We show evidence that LCM, WGA, and NGS of adjacent tumor regions provide an import

151 -cell whole-genome amplification method (LCS-WGA) that can efficiently capture spontaneous DNA damage

152 is inhibited by microinjection of a lectin, WGA, without affecting the normal inactivation of the M-

153 ectin wheat germ agglutinin (WGA) as ligand; WGA inhibits nuclear transport and has been shown to dir

154 vel differentiated human hepatoma cell line (WGA), derived from HepG2, which expresses CYP2B6 and CAR

155 l evaluation of existing methods, a modified WGA strategy was developed that appears to have utility

156 rily resistant to the effects of neurotoxin, WGA-LH(N)/A conjugate potently inhibits secretory respon

157 We therefore developed a novel WGA-independent bioinformatics method called TELR that i

158 ttern of PAG axons in the medulla oblongata, WGA-HRP was injected into the PAG and adjacent tegmentum

159 Hence, the opsonic activity of WGA-Fc effectively modulates fungal cell recognition and

160 Administration of WGA-Fc also dramatically diminished pulmonary inflammati

161 Prophylactic administration of WGA-Fc fully protected mice against H. capsulatum, corre

162 Immunoblot analysis of WGA bound membrane proteins crosslinked with DSS identif

163 Quantitative PCR (qPCR) analysis of WGA products was used as an initial quality assessment o

164 The decreased binding of WGA and HPA to the luminal surface epithelium in human d

165 thodology was tested by measuring binding of WGA to the surface of confluent monolayers of living Cac

166 We show here that the choice of WGA method should be determined by the planned downstrea

167 of a three-part nanoconjugate consisting of WGA-HRP, AuNPs, and drugs for the treatment of diaphragm

168 CX, neuraminidase induced the degradation of WGA-labeled GCX under UF cell culture conditions or in B

169 acts, primarily on the proximal dendrites of WGA-HRP-labeled motoneurons.

170 A heavy density of WGA-HRP-labeled neurons was found in the ipsilateral mes

171 Finally, we found very few examples of WGA-immunoreactive noradrenergic neurons, which suggests

172 Following injections of WGA-Au-HRP into the nuclear LC, triple labeled neurons w

173 amber and anteromedian nucleus injections of WGA-HRP in the same animal.

174 Injections of WGA-HRP into OPt labeled terminals bilaterally in the an

175 Injections of WGA-HRP into the anteromedian nucleus labeled fusiform p

176 Injections of WGA-HRP into the ventral (MGv), dorsal (MGd), or medial

177 Single injections of WGA-HRP or discrete injections of red and green latex mi

178 Large intraocular injections of WGA-HRP were placed into the eye, and patterns of labele

179 roup of animals was given microinjections of WGA-HRP in the medial nucleus of the medial geniculate (

180 Complementary interdigitating patches of WGA-HRP and BDA labeling were found primarily in transit

181 To determine the performance of WGA products on a large-scale genotyping array, we compa

182 A 7- to 10-fold higher proportion of WGA- and anti-PCW-treated cysts than control cysts remai

183 The purity of WGA products was assessed by Sanger sequencing of cloned

184 ed by quality control (QC) on the success of WGA via housekeeping genes.

185 the phenomenon of transsynaptic transfer of WGA-HRP after injection into the olfactory bulb or rats

186 ury there was no intraganglionic transfer of WGA.

187 ously accessing genomic information based on WGA.

188 nsity was assessed by di-4-ANEPPS, FM4-64 or WGA staining using confocal microscopy.

189 s detected in the vast majority of PHA-L- or WGA-positive terminals forming asymmetric synapses.

190 We found that PHA-L- or WGA-positive terminals from tagged VTA cells made asymme

191 termined the presence of VGluT2 in PHA-L- or WGA-positive terminals.

192 radish peroxidase coupled to gold particles (WGA-Au-HRP) or fluorescein-conjugated latex beads, into

193 tify the neurons of origin for this pathway, WGA-HRP injections were centered in the CCS.

194 Two percent WGA-HRP was injected into the lower thoracic/upper lumba

195 utinin conjugated to horseradish peroxidase (WGA-HRP) and fluorescent dyes were made into the medial

196 heat germ agglutinin-horseradish peroxidase (WGA-HRP) and up to four different fluorochromes in V2 la

197 utinin conjugated to horseradish peroxidase (WGA-HRP) and up to four fluorochromes.

198 heat germ agglutinin-horseradish peroxidase (WGA-HRP) as a tracer.

199 germ agglutinin and horseradish peroxidase (WGA-HRP) combined with glutamate and choline acetyltrans

200 heat germ agglutinin-horseradish peroxidase (WGA-HRP) from olfactory sensory neurons to the olfactory

201 heat germ agglutinin-horseradish peroxidase (WGA-HRP) from the ventral hippocampal formation or by an

202 heat germ agglutinin-horseradish peroxidase (WGA-HRP) in dorsal (PMD) and ventral (PMV) premotor area

203 heat germ agglutinin-horseradish peroxidase (WGA-HRP) in the left nodose ganglion.

204 gglutinin-conjugated horseradish peroxidase (WGA-HRP) injections revealed differences in the pattern

205 wheatgerm agglutinin-horseradish peroxidase (WGA-HRP) into ICXv.

206 gglutinin-conjugated horseradish peroxidase (WGA-HRP) into the area of the CCS revealed a distinctive

207 gglutinin-conjugated horseradish peroxidase (WGA-HRP) into the beta-nucleus and dmcc of the inferior

208 gglutinin-conjugated horseradish peroxidase (WGA-HRP) into the muscle.

209 utinin conjugated to horseradish peroxidase (WGA-HRP) into the SC, the heaviest concentrations of lab

210 heat germ agglutinin-horseradish peroxidase (WGA-HRP) or choleragenoid-horseradish peroxidase (B-HRP)

211 heat germ agglutinin-horseradish peroxidase (WGA-HRP) or cytochrome oxidase (CO) histochemistry, afte

212 heat germ agglutinin-horseradish peroxidase (WGA-HRP) placed into the PM resulted in widespread anter

213 heat germ agglutinin-horseradish peroxidase (WGA-HRP) was injected into the lumbosacral cord in three

214 heat germ agglutinin horseradish peroxidase (WGA-HRP) was injected into the NRA in six monkeys.

215 inin conjugated with horseradish peroxidase (WGA-HRP) were made into dorsal/ventral striatum (DS/VS),

216 utinin conjugated to horseradish peroxidase (WGA-HRP) were made into the ferret's superior colliculus

217 utinin conjugated to horseradish peroxidase (WGA-HRP) were placed in caudal (CPB) and rostral (RPB) d

218 inin conjugated with horseradish peroxidase (WGA-HRP) were placed in topographically different locati

219 wheatgerm agglutinin-horseradish peroxidase (WGA-HRP), BDA, or a fluorescent tracer, iontophoreticall

220 heat-germ agglutinin-horseradish peroxidase (WGA-HRP), the carbocyanine dye DiI, and biocytin) to det

221 heat germ agglutinin-horseradish peroxidase (WGA-HRP)-labeled spinocerebellar mossy fiber terminals i

222 utinin conjugated to horseradish peroxidase (WGA-HRP).

223 eat germ agglutinin horse radish peroxidase (WGA-HRP) chemically conjugated to gold nanoparticles (Au

224 of the polymer with a horseradish peroxidase-WGA conjugate.

225 that a nonadherent yolk sac cell population (WGA+, density < 1.077, AA4.1+) can give rise to B cells,

226 Here we present WGA-X, a method based on multiple displacement amplifica

227 ned with a library preparation without prior WGA using an NEBNext Ultra II paired-end kit, which requ

228 Therefore, we propose WGA-Fc as a potential "pan-fungal" therapeutic that shou

229 ecules-of-interest (CRT and a marker protein WGA), and (b) machine-learning (trainable WEKA segmentat

230 s a slight decrease in the total call rates, WGA methods provide a reliable approach for increasing t

231 The resultant WGA-Fc displayed high affinity to purified chitin and bo

232 led with succinyl Triticum vulgare lectin (S-WGA) and represents the major DBA-binding component in T

233 A sensitive WGA-Alexa Fluor 488 fungal staining, surface creation an

234 tated with C-CAM from detergent solubilized, WGA-purified proteins.

235 is by a whole genome amplification strategy (WGA).

236 re and its contributive role in a successful WGA project.

237 Succinylated WGA reveals differences in these axon classes earlier, a

238 on SPA beads, were even more effective than WGA-coated SPA beads for capturing the insect cells.

239 t IB4-HRP was a much less robust tracer than WGA-HRP.

240 These findings highlight the impact that WGA kit selection can have on metagenomic analysis of lo

241 Our results indicate that WGA product performs well on the 250K array compared to

242 The percentages that WGA-HRP retrogradely labelled neurons composed of the pr

243 problem, and earlier studies have shown that WGA samples perform reasonably well in small-scale genet

244 l, and then microarray results verified that WGA from 10(6) cells or approximately 1 ng of genomic DN

245 The WGA-Flashplate is suitable for fully automated high-thro

246 n label the circuits that are engaged by the WGA-expressing damaged neurons.

247 However, we also found the WGA tracer in DRG cell bodies of uninjured sensory neuro

248 n the fluorescence assay but negative in the WGA assay.

249 ches that correct for biases inherent in the WGA procedure and allow for accurate determination of co

250 Mutant BRAF in the WGA-amplified genomic DNA was further amplified by a two

251 Compared to the gDNA method the WGA-based assay had a sensitivity and specificity of 100

252 ophoblasts, short-tandem repeat (STR) of the WGA-amplified content was compared against STR from mate

253 negative in the fluorescence assay while the WGA results for these two kits were ambiguous.

254 genotyping results of genomic DNA and their WGA products from four individuals.

255 This WGA method should readily lend itself to the determinati

256 Three WGA kits were tested for their utility in a metagenomics

257 carboxamide riboside, an AMPK activator, to WGA and human hepatocytes induces CYP2B6 gene expression

258 onditions, three additional proteins bind to WGA-Sepharose and are revealed by the organelle trap ass

259 which were primarily found contralateral to WGA-HRP muscle injections.

260 188-Nup205 complex does not bind directly to WGA but binds indirectly via the N-acetylglucosamine-mod

261 r polyploid genomes that are recalcitrant to WGA and yields new insights into the mechanisms of genom

262 eyes, studied with the transneuronal tracer WGA-HRP, are intermixed in the binocular zone of albinos

263 nilateral injections of a retrograde tracer (WGA-Au, 350-400 nl) were made into the VTA or a nonrewar

264 inase promotes the expression of the tracer, WGA (wheat germ agglutinin), and used these in combinati

265 We used the transganglionic tracers WGA-HRP, IB4-HRP, and CTB-HRP to trace the central proje

266 nd histochemical localization of transported WGA-HRP or B-HRP was performed.

267 xpected, sciatic nerve transection triggered WGA expression in NPY-positive DRG neurons, most of whic

268 es of these ZW (LacZ-WGA) mice, we triggered WGA expression either by crossing the mice with two Cre-

269 The novel TruPrime WGA, based on primase-polymerase features, and WGA-X emp

270 therefore tested the two most commonly used WGA methods, multiple displacement amplification (MDA-Qi

271 sialylated from hyposialylated glycans, used WGA, SNA, PNA, Jacalin, HPA, and VVA, indicating glomeru

272 m retrograde tracing were confirmed by using WGA-HRP as an anterograde tracer from input sources.

273 ntocellularis and magnocellularis, that were WGA-immunoreactive, i.e., were transneuronally labeled f

274 ation and mild fixation were compatible with WGA, although fresh samples delivered better genome qual

275 s study demonstrates that coupling FACS with WGA and microarrays is a promising tool to expedite geno

276 noperated group (controls) was injected with WGA-HRP in the left nodose ganglion.

277 weeks, following intranasal irrigation with WGA-HRP.

278 f AEN trigeminal ganglion cells labeled with WGA-HRP, and (2) electron microscopic analysis of the AE

279 did not react with anti-Hs antibody or with WGA.

280 body reactivity and restores reactivity with WGA.