ライフサイエンスコーパス: Z-line

1 ves of ellipticity 2/1 (major axis along the Z-line).

2 restricted to triadic junctions (located at z-lines).

3 ber axis or pointing toward or away from the Z line.

4 in which Ca(2+) release occurred only at the Z-line.

5 te a broader band of release centered at the Z-line.

6 nd T-cap/telethonin, a 19-kDa protein of the Z-line.

7 e SR around the contractile apparatus at the Z-line.

8 eractions with both a thick filament and the Z-line.

9 nds to alpha-actinin and is localized in the Z-line.

10 The beta2 did not localize to the Z-line.

11 t function that includes interactions at the Z-line.

12 , intercalated disc, and at the level of the Z-line.

13 60 kD of titin spans the entire width of the Z-line.

14 the remaining short thin filaments near the Z-line.

15 obulin-containing proteins in the sarcomeric Z-line.

16 etween the end of the thick filament and the Z-line.

17 ENH-CypherS-Calsarcin protein complex at the Z-line.

18 1 are within the same protein complex at the Z-line.

19 ecting each end of the thick filament to the Z-line.

20 skeletal protein localized in the sarcomeric Z-line.

21 of both calsarcin-1 and myotilin within the Z-line.

22 line provided information about the width of Z lines.

23 oscopy confirms localization specifically to Z lines.

24 binds the barbed ends of actin at sarcomeric Z-lines.

25 fered with localization of endogenous FAK to Z-lines.

26 sis shows DMN colocalized with desmin at the Z-lines.

27 for Ca2+ release at locations other than at z-lines.

28 dhesions, cell-cell junctions, and embryonic Z-lines.

29 ructs such as actin filaments and sarcomeric Z-lines.

30 fect correlation between actin filaments and Z-lines.

31 these same regions also displayed dislocated z-lines.

32 lly attaching the sarcolemma to myofibrillar Z-lines.

33 tially activated along the Z-line and across Z-lines.

34 evere sarcomeric disruptions starting at the Z-line, along with filamentous actin accumulations consi

35 fic "capping protein (actin filament) muscle Z-line, alpha 3" gene (Capza3).

36 The Z-line, alternatively termed the Z-band or Z-disc, is a

37 t muscle is in the M-lines and dense bodies (Z-line analogs).

38 in-binding domain; encoded by exon 6) of the Z-line anchor protein ACTN2 is predominantly excluded fr

39 ltivariate analysis, patients with irregular Z line and patients with BE of >/= 1 cm did not differ s

40 to the perinuclear region, and overlying the Z lines and M bands of adult rat cardiac myocytes.

41 the sarcolemmal domains that lie over nearby Z lines and that also contain beta-spectrin.

42 undles within sarcomeres that interconnected Z lines and were cross-linked by alpha-actinin.

43 poral characteristics of sparks found in the Z-line and A-band zones were very similar, whereas spark

44 seem to be sequentially activated along the Z-line and across Z-lines.

45 containing protein located in the sarcomeric Z-line and I-band.

46 g at Z-lines within the muscle fibers and at Z-line and M-line domains at costameres at the sarcolemm

47 eptide-associated complex protein skNAC as a Z-line and ribosome-associated protein.

48 2 C) between the rise of fluorescence at the z-line and the m-line.

49 ulations with release sites clustered at the Z-lines and a transverse diffusion coefficient 50% of th

50 5) misalignment of alpha-actinin containing Z-lines and abnormal myocardial ultrastructure despite p

51 fluorescence was localized at the sarcomeric z-lines and absent from nuclei.

52 mulations with release sites localized along Z-lines and isotropic diffusion yielded highly elliptica

53 We found perturbations that affected Z-Lines and M-Lines differently, suggesting that they ma

54 showed that PDE1C1 is distributed along the Z-lines and M-lines of cardiac myocytes in a striated pa

55 domain is sufficient for localization to the Z-lines and that if the microtubule network is disrupted

56 muscles, the actin filaments are anchored at Z-lines and the myosin and actin filaments are in regist

57 RPSA localized ribosomes at Z-lines and was trafficked via microtubules.

58 ing cells, with the absence of myofilaments, Z lines, and intercalated disks.

59 s in a double line, flanking desmin over the Z lines, and is apparently in alignment with the termina

60 tively spliced PDZ-motif and myotilin at the Z-line, and an incremental shift towards oxidative metab

61 CPbeta1 attaches actin filaments to the Z-line, and CPbeta2 organizes the actin at the intercala

62 calized in the cytoplasm at the level of the Z-line, and interestingly, FHL2 interacted more efficien

63 to mutations in mitochondrial, cytoskeletal, Z-line, and sarcomeric proteins.

64 ugh this protein colocalizes with BIN1 along Z-lines, and binds all 4 isoforms.

65 rupts dyad architecture, dyad positioning at Z-lines, and junctional sarcoplasmic reticulum Ca(2+) re

66 ensors appear to be localized to the cardiac z-lines, and respond to cumulative calcium transients at

67 ar myocytes, FKBP12.6 and CaM colocalized to Z-lines, and the efficiency of energy transfer to both t

68 s without robust, repeatable tools to assess z-line architecture across different labs and experiment

69 echanism by which sarcomere and by extension z-line architecture can impact contraction and which cha

70 es and choosing metrics to summarize overall z-line architecture have gone largely unaddressed in pre

71 act contraction and which characteristics of z-line architecture should be used to assess striated my

72 metrics that summarize different aspects of z-line architecture that correspond to expert tissue qua

73 or semi-manual methods, characterization of z-line architecture using the metrics discussed and impl

74 e distal esophagus, also called an irregular Z line, are encountered.

75 e distal esophagus, also called an irregular Z line, are encountered.

76 issue of the JCI, Haddad et al. identify the Z-line as a critical site for localized translation of s

77 n-II functions at the muscle termini and the Z-line as an actin crosslinker and acts to maintain the

78 tubules (TTs) that align with sarcomeres and Z-lines as well as longitudinal tubules (LTs) that are p

79 keletal muscle of the rat, anti-p6 decorates Z lines, as defined by antidesmin distribution, and is a

80 wed that these subunits are expressed at the Z lines, as shown previously for Nav1.5.

81 pher is not required for sarcomerogenesis or Z-line assembly, but rather is required for maintenance

82 ered phenotypes ranging from partial loss of Z line-associated filamentous actin to collapse of the c

83 netic interaction with mutants affecting the Z-line-associated protein muscle LIM protein 84B.

84 nregulated in disease, is a key ribosome and Z-line-associated protein responsible for cardiomyocyte

85 the tricarboxylic acid cycle, reductions in Z-line-associated proteins and increases in probe sets f

86 of titin is located at the periphery of the Z-line at the border of the adjacent sarcomere, whereas

87 oducing additional release sites between the Z-lines at a density 20% of that on the Z-lines produced

88 beta1 is at the Z-line; beta2 is at the intercalated disc and cell perip

89 Ig repeats Z1 and Z2 in the periphery of the Z-line bind to a novel 19-kD protein, referred to as tit

90 that nebulin extended 1.01-1.03 mum from the Z-line, but Tmod localized 1.13-1.31 mum from the Z-line

91 Bursts remained localized to individual z-lines, but adjacent sites on the same z-line could be

92 ed the higher release site density along the Z-lines by acting as transverse diffusion barriers and s

93 e nanoscopic microdomains formed adjacent to Z-lines by apposition of transverse tubules and junction

94 ing is suppressed, we estimated that >90% of Z-line CaM is RyR2-bound.

95 odel of release occurring exclusively at the Z-line cannot explain our experimental data and suggest

96 via interactions with alpha-actinin or other Z-line components.

97 Both FKBP12.6 and FKBP12 concentrate at Z-lines, consistent with RyR2 and Ca spark initiation si

98 dual z-lines, but adjacent sites on the same z-line could be 'driven' by a bursting site to generate

99 N-terminal domain of PKC-delta, mimicked the Z-line decoration and slow binding rate of the full-leng

100 None of the patients with irregular Z line developed HGD or EAC during a median follow-up pe

101 study, we found that patients with irregular Z line do not develop HGD or esophageal cancer within 5

102 Alternatively, Z line domains may be lost, whereas domains oriented lon

103 sociates with and selectively stabilizes the Z line domains of costameres, but that cytokeratins asso

104 isrupted sarcomeric structures with punctate Z-lines due to impaired myosin replacement during early

105 ut rather is required for maintenance of the Z-line during muscle function.

106 anchors junctional sarcoplasmic reticulum to Z-lines, establishes dyad architecture, and regulates dy

107 r essential for ribosome localization to the Z-line, facilitating local translation and sarcomere mai

108 R microdomain that was distinct from another Z line-flanking SR microdomain containing Tmod1 and Tmod

109 postnatal mouse heart, BIN1 localizes along Z-lines from early developmental stages, consistent with

110 Challenges such as isolating z-lines from regions of off-target staining that occur a

111 gher proportion of patients in the irregular Z-line group were female (26.3%) than in the BE group (1

112 ower proportion of patients in the irregular Z-line group were smokers (33.5%) than in the BE group (

113 was not observed between sites on different Z-lines (i.e. separated longitudinally by 1.8 microns).

114 We show that Enigma is present at the Z line in skeletal muscle and that the PDZ domain of Eni

115 CYK-1 persisted at Z lines in adulthood, and its near complete depletion fr

116 the actin-binding protein alpha-actinin-2 at Z lines in skeletal muscle.

117 mobility 25 kDa) that localizes to the M and Z lines in skeletal muscle.

118 ncoding a protein that is a component of the Z-line in both skeletal and cardiac muscle.

119 is the major binding site for CaM along the Z-line in cardiomyocytes, and dissociating CaM from RyR2

120 MYPN was localized at the Z-line in control skeletal muscles but was absent from a

121 receptor staining and movement away from the z-line in HF, which was reversed following recovery from

122 nd that nonmuscle myosin-II localizes to the Z-line in mature larval muscle.

123 otherwise inextensible, collapsed toward the Z-line in sarcomeres shorter than approximately 2.0 micr

124 tin, ZIg1 and ZIg2, which are present at the Z-line in situ.

125 ed ubiquitin ligase that is localized to the Z-line in skeletal muscle.

126 rsarcomeric mitochondria concentrated at the Z-line in the IIB fiber types resulting in a periodic pa

127 dinal tubules (LTs) that are present between Z-lines in some species.

128 adhesion proteins Cas, FAK, and paxillin at Z-lines in the cardiac myocyte may regulate, either dire

129 e, but Tmod localized 1.13-1.31 mum from the Z-line, in seven different rabbit skeletal muscles.

130 by recruitment of the titin segment near the Z-line into the elastic pool.

131 d in sarcomeric Z-lines, suggesting that the Z-line is an important signaling locus in these cells.

132 ith FAK, together with their localization to Z-lines, is critical to assembly of sarcomeric units in

133 riated muscle protein that co-localizes with Z-lines, junctional sarcoplasmic reticulum proteins, and

134 its C-terminal region integrates within the Z-line lattice.

135 s localized to discrete release sites at the Z-line level of sarcomeres, indicating that the local OG

136 tribution of all PKD forms in a non-nuclear, Z-line localized, striated reticular pattern, suggesting

137 ricular myocytes, estimate the percentage of Z-line-localized CaM that is RyR2-bound, and test cellul

138 ning with the anti-alpha-actinin antibody (a z-line marker) showed that nearly all GFP-RyR2 clusters

139 y and irregularly in transverse planes along Z-lines (mean spacing between sites of 0.76 microns).

140 AC between groups of patients with irregular Z line (n = 167) and those with BE of >/= 1 cm (n = 1624

141 s in association with alpha-actinin-2 at the Z lines of myofibers.

142 Subsequently, the z lines of the sarcomeres aligned and registered in dist

143 ike MSP-300, are localized to the sarcomeric Z-line of both skeletal and cardiac muscle.

144 oskeleton and tethers actin filaments to the Z-line of the sarcomere in muscles.

145 h tether calcineurin to alpha-actinin at the z-line of the sarcomere of cardiac and skeletal muscle c

146 eight and was diffusely localized around the Z-line of the sarcomere, suggesting a Ca(2+)-dependent m

147 ocalized with alpha-actinin, a marker of the Z-line of the sarcomere.

148 ay enable these heterofilaments to help link Z-lines of adjacent myofibrils and, thereby, play an imp

149 ies, and demonstrated that they label at the Z-lines of both adult avian and porcine cardiac and skel

150 alization of Cypher and alpha-actinin at the Z-lines of cardiac muscle.

151 nd p40 colocalizes with alpha-actinin at the Z-lines of differentiated myotubes.

152 ance of Ca(V) 1.2 'super-clusters' along the z-lines of ISO-stimulated cardiomyocytes.

153 ted expression pattern, especially along the Z-lines of myofibrils.

154 ng protein 3 (LDB3), which is located at the Z-lines of the sarcomere, at different times during the

155 'Z-Bodies" of sarcomere precursors and the 'Z-Lines' of sarcomeres, and has been used previously to

156 aline-like bodies adjacent to a disorganized Z-line on electron microscopy, recapitulating the diseas

157 found GFP-RyR2 clusters interspersed between z-lines only at the periphery of live ventricular myocyt

158 NH2-terminal or I-band regions of titin, the Z-lines, or the thin filaments.

159 the muscle cytoskeleton and occur along the Z lines owing to interaction of the PDZ domain with the

160 c development, CMYA5 positioning adjacent to Z-lines precedes junctional sarcoplasmic reticulum posit

161 the Z-lines at a density 20% of that on the Z-lines produced circular waves.

162 an alpha-actinin- and gamma-filamin-binding Z line protein expressed predominantly in skeletal muscl

163 Thus, myozenin is a skeletal muscle Z line protein that may be a good candidate gene for lim

164 contains a binding site for the myofibrillar Z-line protein alpha-actinin.

165 MYPN encodes the Z-line protein myopalladin implicated in sarcomere integ

166 Furthermore, ZASP-GFP, a Z-line protein, colocalizes with F-actin in puncta at th

167 with alpha-actinin, an integral myofibrillar Z-line protein.

168 identified ribosomal protein SA (RPSA) as a Z-line protein.

169 er, ENH interacted with Calsarcin-1, another Z-line protein.

170 y immunostaining of alpha-actinin, a cardiac z-line protein.

171 structure is often evaluated by staining for z-line proteins such as alpha-actinin.

172 ough protein-protein interactions with other Z-line proteins, (ii) myocardial ablation of Cypher resu

173 er) of the PDZ-LIM domain protein family are Z-line proteins.

174 y, phallacidin fluorescence intensity at the Z line provided information about the width of Z lines.

175 s (12 from its central region and 3 from its Z-line region).

176 xhibits disorganization and streaming of the Z-line similar to that seen in nemaline myopathy.

177 binding sites for alpha-actinin within their Z-line spanning region.

178 Electron microscopy showed areas with Z-line streaming and a dilated sarcotubular system with

179 ia, deeply infolded sarcolemma, and frequent Z-line streaming regions, which were more severe in male

180 ASP1-D117N did not significantly disturb the Z-line structure.

181 ad moving within the sarcomere away from the Z-line structures and leaving behind the sarcoplasmic re

182 s, and paxillin were localized in sarcomeric Z-lines, suggesting that the Z-line is an important sign

183 Internal CRUs were more tightly packed along z-lines than surface CRUs, contained larger and more num

184 ine the molecular layout of titin within the Z-line; the most NH2-terminal 30 kD of titin is located

185 Decreased sarcomere length and increased Z-line thickness were observed in the mutant hearts cons

186 Single molecules of titin extend from the Z-line to the M-line.

187 ound inside the sarcomere, spanning from the Z-line to the M-line.

188 of myomesin and a protein that connects the Z-Lines to the M-Line (titin).

189 parameters were adjusted until the simulated Z-line transient matched the experimental one.

190 ugh the model was accurate in predicting the Z-line transient under conditions of high [EGTA], it pre

191 ct key parameters of the experimental M- and Z-line transients, even when model parameters were adjus

192 in in the internal solution localized at the Z-line/triad region.

193 ue roles for CypherL isoforms in maintaining Z-line ultrastructure and signaling that are distinct fr

194 from these mutants exhibited defects in both Z-line ultrastructure and specific aberrations in calcin

195 are respectively higher and faster near the Z-line versus M-line.

196 [Ca(2+)](mito) near SR Ca(2+) release sites (Z-line) versus mid-sarcomere (M-line) reached a high pea

197 within Cypher localize independently to the Z-line via interactions with alpha-actinin or other Z-li

198 that synemin may anchor IFs to myofibrillar Z-lines via interactions with alpha-actinin and to costa

199 aned' in HF, although RyR distribution along Z-lines was relatively sparse.

200 ter understand the structure and function of Z lines, we used sarcomeric isoforms of alpha-actinin an

201 iated body wall muscles near or on sarcomere Z lines, where barbed ends of actin filaments are anchor

202 ransferase (OGT) are mainly localized at the Z-line, whereas OGA is at the A-band.

203 0-nm-long thin filaments protruding from the Z-line, whereas the rest of the I-band became devoid of

204 co-localized with ryanodine receptors at the Z-lines, whereas in myopathic hearts the degree of co-lo

205 The segment of titin near the Z-line, which is otherwise inextensible, collapsed towar

206 ning, MYPN was found to bind to titin in the Z-line, which was confirmed by microscale thermophoresis

207 sarcomeres is ideally perpendicular to their z-lines, which couple parallel myofibrils and give cardi

208 Titin filaments may specify Z-line width and internal structure by varying the lengt

209 ce of HGD and EAC in patients with irregular Z line with intestinal metaplasia.

210 ling and enhanced the alignment of sarcomere Z-lines with ryanodine receptor 2, a protein that mediat

211 ui, like CaMKIIdelta, is concentrated at the z-lines, with low baseline activation state.

212 antibodies for K8 and K19 showed labeling at Z-lines within the muscle fibers and at Z-line and M-lin

213 h the zone of the junctional or corbular SR (Z-line zone).

214 all GFP-RyR2 clusters were localized in the z-line zone.