ライフサイエンスコーパス: acetoacetyl

1 3-Acetoacetyl-4,6-diaryl-2-pyridones are synthesized in th

2 er protein (ACP) to make the FAS primer beta-acetoacetyl-ACP in the accompanying article.

3 The radiolabeled acetoacetyl-ACP product is precipitated and separated fr

4 ein (ACP) synthase III (KAS III, also called acetoacetyl-ACP synthase) encoded by the fabH gene is th

5 ACP) allows the generation of a biotinylated acetoacetyl-ACP.

6 ularly against the KASIII domain of the beta-acetoacetyl-acyl carrier protein (ACP) synthase FabH.

7 alonyl-CoA derived extender unit to yield an acetoacetyl-, beta-ketopentanoyl-, 3-oxo-4-methylpentano

8 role of AACT2 in generating the bulk of the acetoacetyl CoA precursor required for the cytosol-local

9 Acetoacetyl CoA thiolase (AACT, EC 2.3.1.9) catalyzes th

10 , acetyl CoA, crotonoyl CoA, n-propzoyl CoA, acetoacetyl CoA, malonyl CoA) were completely separated

11 ensation of two acetyl CoA molecules to form acetoacetyl CoA.

12 by monitoring the use of a second substrate, acetoacetyl-CoA (300 nm).

13 n solution, where HMG-CoA is cleaved to form acetoacetyl-CoA (AcAc-CoA) and acetate.

14 netic and chemical mechanisms of KACPR using acetoacetyl-CoA (AcAc-CoA) as a substrate.

15 imilar K(m) values for binding of substrates acetoacetyl-CoA (K(m) 9.8 +/- 0.8 microM) and CoA (K(m)

16 DH II inhibited, in parallel, reduction of S-acetoacetyl-CoA (Ki approximately 1.6 microM), as well a

17 tructure of E170Q in complex with NAD(+) and acetoacetyl-CoA (R = 21.9%, R(free) = 27.6%, 2.2 A) reve

18 ossessed virtually unchanged K(m) values for acetoacetyl-CoA and CoA but had a greater than 99% decre

19 t in opposite directions) for the binding of acetoacetyl-CoA and indoleacryloyl-CoA to the enzyme.

20 h apparent Km values of 89 and 20 microM for acetoacetyl-CoA and NADH, respectively.

21 eneral base both in the condensation between acetoacetyl-CoA and the acetylated enzyme, and the hydro

22 The acetoacetyl-CoA binary structure demonstrates reduced co

23 contrast, abstraction of the alpha-proton of acetoacetyl-CoA by Arg-94 --> Gln mutant dehydrogenase i

24 ed HMGS from Staphylococcus aureus and bound acetoacetyl-CoA by cryo-cooling enzyme crystals at three

25 off-rate" of acetoacetyl-CoA from the enzyme-acetoacetyl-CoA complex.

26 argely attributed to a decreased affinity of acetoacetyl-CoA for these enzymes and, more specifically

27 n increase in the dissociation "off-rate" of acetoacetyl-CoA from the enzyme-acetoacetyl-CoA complex.

28 A dehydrogenase complexed with the inhibitor acetoacetyl-CoA has been determined at 2.25 A resolution

29 Succinyl-CoA and acetoacetyl-CoA increased the rate of glycine proton rem

30 catalyzes the condensation of acetyl-CoA and acetoacetyl-CoA into 3-hydroxy-3-methylglutaryl CoA.

31 athway is the condensation of acetyl-CoA and acetoacetyl-CoA into HMG-CoA, catalyzed by the enzyme HM

32 initially reacts with acetoacetate to yield acetoacetyl-CoA plus succinate in the succinyl-CoA-aceto

33 rmational states with NADPH and NADP(+) plus acetoacetyl-CoA present, including structures with the A

34 This would be followed by acetoacetyl-CoA reacting with acetyl-CoA to generate HMG

35 catalyzed by a beta-ketothiolase (PhaA), an acetoacetyl-CoA reductase (PhaB), and a polyhydroxyalkan

36 etic fragment encoding beta-ketothiolase and acetoacetyl-CoA reductase behind a modified synthase gen

37 haB2 and phaB3 as well as 15 other potential acetoacetyl-CoA reductases.

38 se-catalyzed condensation of acetyl-CoA with acetoacetyl-CoA requires enolization/carbanion formation

39 analyses showed limited activity against an acetoacetyl-CoA substrate in vitro.

40 ydrogenase in cultured skin fibroblasts with acetoacetyl-CoA substrate showed reduced activity.

41 which, unlike many organisms, also exhibits acetoacetyl-CoA synthetase (KBC) activity.

42 zation, and a new acetylation target, namely acetoacetyl-CoA synthetase (SlAacS).

43 However, lowering acetoacetyl-CoA synthetase 80% partially inhibited gluco

44 sent a conserved mechanism for regulation of acetoacetyl-CoA synthetase activity in all domains of li

45 succinyl-CoA:3-ketoacid-CoA transferase and acetoacetyl-CoA synthetase to synthesize and use acetoac

46 forms acetoacetate in the mitochondria, and acetoacetyl-CoA synthetase, which uses acetoacetate to f

47 ivo evidence show that SlAacS is a bona fide acetoacetyl-CoA synthetase.

48 hree genes encoding JH biosynthetic enzymes, acetoacetyl-CoA thiolase (thiolase), farnesyl diphosphat

49 d more forcefully to regulatory stimuli than acetoacetyl-CoA thiolase activity but usually less than

50 6.5-kDa mvaE gene product catalyzed both the acetoacetyl-CoA thiolase and HMG-CoA reductase reactions

51 Under conditions at which acetoacetyl-CoA thiolase and long-chain thiolase were co

52 g as a selective SNO-CoA reductase, protects acetoacetyl-CoA thiolase from inhibitory S-nitrosylation

53 Kinetic studies of acetoacetyl-CoA thiolase implicated a ping-pong mechanis

54 s demonstrated by using HMG-CoA synthase and acetoacetyl-CoA thiolase/HMG-CoA reductase from E. faeca

55 d mitochondrial thiolases, as well as to the acetoacetyl-CoA thiolases of prokaryotes.

56 the kinetics of the reaction that have shown acetoacetyl-CoA to be a potent inhibitor of the overall

57 r) is crotonyl-CoA reductase, which converts acetoacetyl-CoA to butyryl-CoA for use as a 4C extender

58 acetyl-CoA, which precedes condensation with acetoacetyl-CoA to form the HMG-CoA product.

59 the DeltaH degrees value for the binding of acetoacetyl-CoA to the enzyme was 5.6 kcal/mol more favo

60 a crystal structure of HMG-CoA synthase with acetoacetyl-CoA was determined at 2.5-A resolution.

61 differences in (13)C NMR chemical shifts for acetoacetyl-CoA when bound as an enolate to MCAD and eno

62 catalyzed the NADH-dependent reduction of S-acetoacetyl-CoA with a Km of approximately 68 microM and

63 r H264 interacts with the carbonyl oxygen of acetoacetyl-CoA's thioester, turnover of S-(3-oxobutyl)-

64 of acetyl phosphate, acetoacetyl coenzyme A (acetoacetyl-CoA), butyryl CoA, acetoacetate, and beta-hy

65 st that, in the absence of second substrate (acetoacetyl-CoA), enzymatic addition of H(2)(18)O to the

66 nt types of CoA-ligands (viz., octenoyl-CoA, acetoacetyl-CoA, and indoleacryloyl-CoA) to human liver

67 rporate carbon from glucose into acetyl-CoA, acetoacetyl-CoA, and succinyl-CoA and carbon from leucin

68 le substrate analogs, 3-thiaglutaryl-CoA and acetoacetyl-CoA, are not altered by the mutations.

69 ay a role in anchoring the second substrate, acetoacetyl-CoA, by interacting with the carbonyl oxygen

70 in formation of a stable binary complex with acetoacetyl-CoA, F204L exhibits binding stoichiometries

71 of the purine and nicotinamide nucleotides, acetoacetyl-CoA, H2O2, reduced glutathione, and 2-monoac

72 ndensation of two molecules of acetyl-CoA to acetoacetyl-CoA, is thermodynamically unfavorable.

73 ondria and enzymes that can form acetyl-CoA, acetoacetyl-CoA, malonyl-CoA, and HMG-CoA in their cytos

74 ass spectrometry measurements of acetyl-CoA, acetoacetyl-CoA, succinyl-CoA, hydroxymethylglutaryl-CoA

75 s acutely stimulated 1.5-5-fold increases in acetoacetyl-CoA, succinyl-CoA, malonyl-CoA, hydroxymethy

76 In the case of acetoacetyl-CoA, the spectrum of the enzyme-ligand compl

77 enzyme complexed with its second substrate, acetoacetyl-CoA, to 1.9 A.

78 of S-(3-oxobutyl)-CoA, a thioether analog of acetoacetyl-CoA, was investigated.

79 acetyl-S-enzyme during its condensation with acetoacetyl-CoA.

80 y E95Q was not stimulated in the presence of acetoacetyl-CoA.

81 ernary complex of the enzyme with NAD(+) and acetoacetyl-CoA.

82 of S-3-oxobutyl-CoA, the thioether analog of acetoacetyl-CoA.

83 roximately 100-fold increase in the k(m) for acetoacetyl-CoA.

84 ensitive to feedback substrate inhibition by acetoacetyl-CoA.

85 nsible for the formation of butyryl-CoA from acetoacetyl-CoA.

86 arent k(m) values for S-(3-oxobutyl)-CoA and acetoacetyl-CoA.

87 obtained using the physiological substrate, acetoacetyl-CoA.

88 Escherichia coli cells that overexpress the acetoacetyl-CoA:acetyl-CoA transferase, AtoAD (EC 2.8.3.

89 h microsomal HMG-CoA reductase and cytosolic acetoacetyl coenzyme A (AcAc-CoA) thiolase activities.

90 e, and increased levels of acetyl phosphate, acetoacetyl coenzyme A (acetoacetyl-CoA), butyryl CoA, a

91 oding beta-ketothiolase (phaA), NADPH-linked acetoacetyl coenzyme A (acetyl-CoA) reductase (phaB), an

92 strate that FadA5 catalyzes the thiolysis of acetoacetyl-coenzyme A (CoA).

93 obacterium tuberculosis and its complex with acetoacetyl-coenzyme A at 1.8 and 2.3 A resolution, resp

94 express in transgenic cotton genes encoding acetoacetyl-coenzyme A reductase and polyhydroxyalkanoic

95 ired activation of acetoacetate by cytosolic acetoacetyl-coenzyme A synthetase (AACS).

96 ncode two enzymes of the mevalonate pathway, acetoacetyl-coenzyme A thiolase and 3-hydroxy-3-methylgl

97 the Lewis acids and diketene, monoprotonated acetoacetyl fluoride and diprotonated acetoacetyl fluori

98 Monoprotonated acetoacetyl fluoride is characterized by a six-membered

99 onated acetoacetyl fluoride and diprotonated acetoacetyl fluoride were obtained.

100 haracterizations of iso-CoA, acetyl-iso-CoA, acetoacetyl-iso-CoA, and beta-hydroxybutyryl-iso-CoA usi

101 ase (PKS-NRPS) that makes and releases cyclo-acetoacetyl-L-tryptophan (cAATrp), the tetramic acid tha

102 e synthetase (PKS-NRPS) that generates cyclo-acetoacetyl-L-tryptophan (cAATrp).

103 ctures including 4 novel modifications: N(6)-acetoacetyl lysine, N(6)-isovaleryl lysine, N(6)-(2-meth

104 an acetate-derived beta-methyl branch on an acetoacetyl-S-carrier protein and ultimately generate a

105 alyzes a Dieckmann-type cyclization on the N-acetoacetyl-Trp intermediate bound in thioester linkage