ライフサイエンスコーパス: acetoxymethyl

1 d compounds, 5-nitro-2-furoic acid (5NFA), 5-acetoxymethyl-2-furaldehyde (5AMF), and methyl-5-nitro-2

2 Acetyl-L-alanyl-gamma-D-glutamyl-L-lysine]-3-acetoxymethyl-3-ceph em-carboxylic acid (compound 6), wa

3 Furthermore, the 4-acetoxymethyl-3-methoxy-4-methyl-5-trimethylsilyl-2,5-cy

4 The 4-acetoxymethyl-4-alkyl-3-trimethylsilyl-2,5-cyclohexadien

5 BAPTA is delivered to the cytoplasm as the acetoxymethyl (AM) ester BAPTA/AM, but released AM group

6 s involving bulk loading of neurons with the acetoxymethyl (AM) ester version of Cal-590, combined tw

7 by masking the carboxylate of RhoVR 1 as an acetoxymethyl (AM) ester.

8 The study demonstrated that Calcein acetoxymethyl (AM) proved to be a suitable dye for detec

9 pHi was recorded fluorimetrically with acetoxymethyl (AM)-loaded carboxy-SNARF-1 at either 27 o

10 reacted with lead tetraacetate to give a bis(acetoxymethyl) derivative that condensed with an alpha-u

11 s doors to avoid using labile esters such as acetoxymethyl derivatives which are not optimal in imagi

12 rabbit ventricular myocytes loaded with the acetoxymethyl ester (AM) form of carboxy-seminaphthorhod

13 Myotubes loaded with the acetoxymethyl ester (AM) form of fluo-3 were imaged at r

14 of free cytosolic [Ca2+] ([Ca2+]c) using the acetoxymethyl ester (AM) form of indo-1 may be compromis

15 ing by cell-permeable Ca(2+) chelators [EGTA-acetoxymethyl ester (AM), 1, 2-bis(2-aminophenoxy)ethane

16 rated that, like MDR1, SPGP effluxed calcein-acetoxymethyl ester (AM).

17 on flexible membranes and loaded with Fura-2 acetoxymethyl ester (AM).

18 r esterase activity were analyzed by calcein-acetoxymethyl ester (AM)/ethidium homodimer assay.

19 + buffer, 2-aminophenol-N,N,O-triacetic acid acetoxymethyl ester (APTRA-AM, 20-40 mg/kg), on synaptic

20 inophenoxy)ethane-N,N,N',N'-tetraacetic acid-acetoxymethyl ester (BAPTA-AM) and completely eliminated

21 inophenoxy)ethane-N,N,N',N'-tetraacetic acid-acetoxymethyl ester (BAPTA-AM) and decreased by 1.7-fold

22 phenoxy)ethane-N, N, N', N'-tetraacetic acid-acetoxymethyl ester (BAPTA-AM) reduced cytosolic Ca(2+)

23 inophenoxy)ethane-N,N,N',N'-tetraacetic acid acetoxymethyl ester (BAPTA-AM), a Ca2+ chelator.

24 minophenoxy)ethane-N,N,N',N'-tetraacetate-AM acetoxymethyl ester (BAPTA-AM), cyclosporine, and inhibi

25 iniphenoxy)ethane-N,N,N',N'-tetraacetic acid acetoxymethyl ester (BAPTA-AM); and (4) after pretreatme

26 2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM), that accumulates only in

27 in culture media containing 2 microM calcein-acetoxymethyl ester (calcein-AM) and 4 microM ethidium h

28 toma cells was evaluated with 4-hour calcein acetoxymethyl ester (calcein-AM) microcytotoxicity assay

29 o)-2'-O-methyladenosine-3',5'-monophosphate, acetoxymethyl ester (cpTOME) increased Socs3 expression

30 Ca2+ imaging studies using fura-2 acetoxymethyl ester (fura-2 AM) revealed that the increa

31 solic Ca2+ as measured by single-cell fura-2 acetoxymethyl ester (Fura-2) Ca2+-dependent fluorescence

32 y-2-naphthalenyl methyl phosphonic acid tris acetoxymethyl ester (HNMPA-(AM)(3)) inhibitor diminished

33 Here, we show that NAADP acetoxymethyl ester (NAADP-AM), a cell-permeant NAADP an

34 pig cardiac myocytes were loaded with rhod-2-acetoxymethyl ester (rhod-2 AM), and [Ca2+]c was monitor

35 nt pH indicator carboxy seminaphtorhodafluor acetoxymethyl ester acetate, we measured pH(c) in the ce

36 In particular, Sulfidefluor-7 acetoxymethyl ester allows for direct, real-time visuali

37 nophenoxy)ethane-N,N,N', N'-tetraacetic acid acetoxymethyl ester also inhibited cytokine TNFalpha-ind

38 nophenoxy)ethane-N,N,N', N'-tetraacetic acid acetoxymethyl ester and EGTA acetoxymethyl ester and the

39 etraacetic acid acetoxymethyl ester and EGTA acetoxymethyl ester and the Ser/Thr phosphatase inhibito

40 , cell survival was quantified using calcein-acetoxymethyl ester compound and a fluorescent plate rea

41 d into arterioles by pre-incubation with the acetoxymethyl ester derivative.

42 th the sodium-binding bezofuran isophthalate acetoxymethyl ester fluorescence indicator.

43 )](i)), acini were incubated in fura-2 tetra-acetoxymethyl ester for 60 minutes at 22 degrees C, and

44 ent by buffering intracellular Ca2+ with the acetoxymethyl ester form of BAPTA (BAPTA AM).

45 ing by noradrenaline, OAG, PDBu, CPA and the acetoxymethyl ester form of BAPTA (BAPTA-AM) was markedl

46 of SOC activity evoked by either CPA or the acetoxymethyl ester form of BAPTA (BAPTA-AM).

47 ously cannot be attributed to the use of the acetoxymethyl ester form of fura-2 to report [Ca2+]i, an

48 of 2.2 micron in which the membrane permeant acetoxymethyl ester form of fura-2 was used.

49 ated from ferret or cat were loaded with the acetoxymethyl ester form of indo-1 (indo-1 AM) such that

50 ccomplished by incubating the cells with the acetoxymethyl ester forms (benz2 AM or BAPTA AM).

51 2-aminophenoxy)ethane-N,N,N',N'-tetraacetate/acetoxymethyl ester has similar effects to water immersi

52 inositol-1,4,5-trisphosphate (IP(3)) hexakis acetoxymethyl ester having an ortho-nitroveratryl photoc

53 O-methyladenosine 3',5'-cyclic monophosphate acetoxymethyl ester mimicked all butaprost effects.

54 erved when the fibers were preincubated with acetoxymethyl ester of 1,2-bis (2-amino-phenoxy) ethane

55 ngendorff perfused rabbit hearts loaded with acetoxymethyl ester of 1,2-bis(2-amino-5,6-difluoropheno

56 The bis-acetoxymethyl ester of 3-phosphonofluorescein readily en

57 but not by calcium chelation with BAPTA-AM (acetoxymethyl ester of BAPTA) (75 microM for 30 min), su

58 xposure of the cone to the membrane-permeant acetoxymethyl ester of the Ca2+ chelator BAPTA.

59 inophenoxy)ethane-N,N,N',N'-tetraacetic acid/acetoxymethyl ester or thapsigargin.

60 Pretreatment with either EGTA acetoxymethyl ester or vitamin E resulted in a significa

61 inophenoxy)ethane-N,N,N',N'-tetraacetic acid acetoxymethyl ester produced a > 2-fold reduction in VP-

62 inophenoxy)ethane-N,N,N',N'-tetraacetic acid acetoxymethyl ester significantly decreased ET-1dependen

63 eceptor antagonists, was measured by calcein-acetoxymethyl ester staining after 3 days in culture.

64 vival rates of RGCs were measured by calcein-acetoxymethyl ester staining.

65 henoxy)ethane-N',N'-tetraacetic acid (BAPTA)-acetoxymethyl ester to buffer intracellular calcium resu

66 ith the fluorescent calcium indicator fura-2 acetoxymethyl ester to measure their intracellular Ca(2+

67 ith the fluorescent calcium indicator Fura-2 acetoxymethyl ester to measure their intracellular Ca2+

68 Application of membrane-permeant NAADP acetoxymethyl ester to PASMCs elicited a biphasic increa

69 yte-like U937 cells were labeled with fluo-3-acetoxymethyl ester to quantitate intracellular Ca2+ con

70 '-bis(2-carboxyethyl)-5,6-carboxyfluorescein-acetoxymethyl ester was used to quantitate intracellular

71 nophenoxy)ethane-N,N,N',N'-tetraacetic acid (acetoxymethyl ester) (BAPTA AM) did the opposite.

72 ethane-N,N, N',N'-tetraacetic acid tetrakis (acetoxymethyl ester) (BAPTA-AM) blocked both ERK and Ras

73 ethane-N,N,N',N'-tetra-acetic acid tetrakis (acetoxymethyl ester) (BAPTA-AM) or blockade of extracell

74 nophenoxy)ethane-N,N,N',N'-tetraacetic acid (acetoxymethyl ester) (BAPTA-AM) or the three specific ca

75 )ethane-N,N,N',N'-tetraacetic acid tetrakis (acetoxymethyl ester) (BAPTA-AM), an intracellular Ca(2+)

76 y)ethane-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl ester) (BAPTA-AM), leads to a dramatic red

77 )ethane-N,N,N',N'-tetraacetic acid-tetrakis (acetoxymethyl ester) (BAPTA-AM).

78 simultaneous measurements of [Ca2+]i (fluo-3-acetoxymethyl ester) and membrane currents/action potent

79 y)ethane-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl ester) and the phospholipase C inhibitor U

80 noxy)ethane-N,N,N',N'-tetraacetic acid tetra(acetoxymethyl ester) had no effect on the serotonin-indu

81 )ethane-N,N,N',N'-tetraacetic acid tetrakis (acetoxymethyl ester) inhibited the process by 50%.

82 y)ethane-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl ester) or down-regulation of CaMKII by KN-

83 y)ethane-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl ester) reduced cAMP levels in PC1-knock-ou

84 ) and intracellular Ca(2+) signaling (fluo-3-acetoxymethyl ester) were recorded simultaneously in myo

85 aminophenoxy)ethane-N,N,N,N-tetraacetic acid acetoxymethyl ester), an intracellular calcium chelator.

86 nophenoxy)ethane-N,N,N',N'-tetraacetic acid (acetoxymethyl ester), ROS scavengers, and the caspase in

87 inophenoxy)ethane-N,N,N',N'-tetraacetic acid acetoxymethyl ester), whereas activation of ERK was not

88 photo-activatable fluorophore, NPE-HCCC2/AM (acetoxymethyl ester); (ii) locally photolyzing a caged c

89 ethane-N,N,N',N'-tetraacetic acid tetrakis (acetoxymethyl ester)] treatments.

90 minophenoxy)ethaneN,N,N',N'-tetraacetic acid acetoxymethyl ester, an inhibitor of intracellular calci

91 inophenoxy)ethane-N,N,N',N'-tetraacetic acid-acetoxymethyl ester, an intracellular Ca(2+) chelator, b

92 inophenoxy)ethane-N,N,N',N'-tetraacetic acid acetoxymethyl ester, and by the guanyl cyclase inhibitor

93 oaded with the calcium indicator dye, fluo-3 acetoxymethyl ester, and fluorescence was measured by la

94 tion, cells were incubated with Fura 2 tetra acetoxymethyl ester, and intracellular [Ca(2+)] ([Ca(2+)

95 S indicators, Sulfidefluor-4, Sulfidefluor-5 acetoxymethyl ester, and Sulfidefluor-7 acetoxymethyl es

96 loaded with a calcium indicator dye, fluo-3 acetoxymethyl ester, and the fluorescence was measured b

97 smooth muscle cells were loaded with fluo-3 acetoxymethyl ester, and the fluorescence was recorded b

98 tolysis of the caged Ca(2+) chelator diazo-2-acetoxymethyl ester, arrests mitosis.

99 inophenoxy)ethane-N,N,N',N'-tetraacetic acid/acetoxymethyl ester, BAPTA/AM) fully inhibits intracellu

100 mitochondrial Ca(2+) uniporter, or with EGTA acetoxymethyl ester, but not with vitamin E, prevented t

101 inophenoxy)ethane-N,N,N',N'-tetraacetic acid acetoxymethyl ester, cyclopiazonic acid, and N,N,N',N'-t

102 red rat forebrain neurons loaded with indo-1 acetoxymethyl ester, KB-R7943 inhibited the reverse mode

103 Through its acetoxymethyl ester, Nmoc-DBHQ can be loaded into cells

104 lular calcium mobilization assay with fura-2 acetoxymethyl ester, peritoneal macrophages from wild-ty

105 inophenoxy)ethane-N,N,N',N'-tetraacetic acid-acetoxymethyl ester, removal of extracellular Ca(2+), th

106 inophenoxy)ethane-N,N,N',N'-tetraacetic acid-acetoxymethyl ester, the adenylate cyclase stimulant for

107 2-carboxyethyl)-5-(and-6)-carboxyfluorescein-acetoxymethyl ester, the initial rates of monocarboxylat

108 ponin or alpha-toxin the dye, loaded via its acetoxymethyl ester, was predominantly trapped in the sa

109 or-5 acetoxymethyl ester, and Sulfidefluor-7 acetoxymethyl ester, which offer the unique capability t

110 In fura-2-acetoxymethyl ester-loaded alveolar macrophages, intrace

111 Ca2+ concentration was equivalent in Fura-2 acetoxymethyl ester-loaded Q212L-alpha 16 cells compared

112 '-bis(2-carboxyethyl)-5(6)carboxyfluorescein-acetoxymethyl ester.

113 with the Ca(2+) fluorescent indicator Fura-2 acetoxymethyl ester.

114 with the fluorescent Ca(2+) indicator fluo-4 acetoxymethyl ester.

115 ]ethane-N,N,N',N'-tetraacetic acid tetrakis [acetoxymethyl ester]).

116 noxy)ethane-N,N,N',N'-tetraacetic acid tetra(acetoxymethyl) ester (BAPTA-AM) or inhibiting NO synthas

117 y)ethane-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl) ester (BAPTA-AM) or the CaM antagonist W7

118 xy) ethane-N,N,N',N'-tetraacetic acid tetra (acetoxymethyl) ester (BAPTA-AM) reduced 5-HT release.

119 noxy)ethane-N,N,N',N'-tetraacetic acid tetra(acetoxymethyl) ester (BAPTA-AM) was added in some studie

120 noxy)ethane-N,N,N',N'-tetraacetic acid-tetra(acetoxymethyl) ester (BAPTA-AM), and PI3-K inhibitor (LY

121 nophenoxy)ethane-N,N,N',N'-tetraacetic acid-(acetoxymethyl) ester (BAPTA-AM), indicating that Ca(2+)

122 y)ethane-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl) ester, 0.1 micromol/l), or by inhibiting

123 s (o-aminophenoxy) ethane-tretraacetic acid (acetoxymethyl) ester, and dantrolene, did not alter bort

124 oxy)ethane-N,N,N', N'-tetraacetic acid/tetra(acetoxymethyl) ester, protected the cells against injury

125 yl-myo-inositol 1,3, 4-trisphosphate-hexakis(acetoxymethyl) ester.

126 nophenoxy)ethane-N,N,N',N'-tetraacetic acid (acetoxymethyl) ester.

127 phenoxy)ethane-N,N,N,N',N'-tetraacetic acid (acetoxymethyl)ester (BAPTA-AM) abolished aggregation ind

128 noxy)ethane-N,N,N',N'-tetraacetic acid tetra(acetoxymethyl)ester (BAPTA-AM).

129 o-inositol 3,4,5,6-tetrakisphosphate octakis(acetoxymethyl)ester (Bt(2)Ins (3,4,5,6)P(4)/AM).

130 -8-bis-(carboxymethyl)-aminoquinoline tetra-(acetoxymethyl)ester (Quin/AM), a calcium chelator, or wi

131 nophenoxy)ethane-N,N,N',N'-tetraacetic acid (acetoxymethyl)ester.

132 enoxy)ethane-N,N,N',N'tetraacetic acid tetra(acetoxymethyl)ester.

133 nyl)-ethane-N,N,N',N'-tetraacetic acid tetra(acetoxymethyl)-ester, a chelator of intracellular Ca2+.

134 oxy)ethane-N,N,N',N'-tetraacetic acid, tetra(acetoxymethyl)-ester, a membrane-permeable calcium chela

135 xy)-ethane-N,N,N',N'-tetraacetic acid tetra-(acetoxymethyl)-ester.

136 BAPTA) or pretreatment of oocytes with BAPTA-acetoxymethyl-ester (BAPTA-AM) nearly completely prevent

137 ',7'-bis-2-carboxyethyl-5-carboxyfluorescein-acetoxymethyl-ester (BCECF).

138 The acetoxymethyl esterified sensor variant was readily take

139 amps) to validate the selectivity with which acetoxymethyl esters (AM-esters) of cAMP analogs prefere

140 he isolated fiber cells were loaded with the acetoxymethyl esters of Fluo-3 or Calcium Green-2, or wi

141 -C8-PIP3/AM and di-C12-PIP3/AM, the heptakis(acetoxymethyl) esters of dioctanoyl- and dilauroylphosph

142 a sterically bulky cyclopropylmethyl-derived acetoxymethyl ether renders carboVF weakly fluorescent;

143 , plasma membrane integrity based on calcein-acetoxymethyl fluorescence was significantly greater at

144 ory aptitude and resulted in corresponding N-acetoxymethyl-, N-alkoxymethyl-, and N-hydroxymethyl-N-a

145 re tagged by uptake of one of the following: acetoxymethyl of 2',7'-bis-(2-carboxyethyl)-5-(and 6)-ca

146 oacridine conjugate of a silyl-protected bis(acetoxymethyl)phenol (bisQMP) was synthesized and evalua

147 inophenoxy)ethane-N,N,N',N'-tetraacetic acid-acetoxymethyl) prevented the CT-induced disappearance of

148 cale-up reaction, and synthetic utility of N-acetoxymethyl products was demonstrated by transforming

149 etate derivative, 5-[(acetylamino)methyl]-2-(acetoxymethyl)pyridine (37).

150 While 3'-acetoxymethyl-substituted cephalosporins are readily ava

151 Viable cells were labeled with calcein acetoxymethyl, visualized using fluorescent microscopy,

152 present series is compound 8b [(1S,3R,6S)-2-(acetoxymethyl)-(Z)-9-(3, 4-dichlorophenylmethylene)-7-az