ライフサイエンスコーパス: acid ceramidase

1 min reduced transcriptional up-regulation of acid ceramidase.

2 D2646, to inhibit the hydrolytic activity of acid ceramidase.

3 ped to produce and purify recombinant, human acid ceramidase.

4 These data indicate that ASAH1 (acid ceramidase 1) and GBA2 (glucocerebrosidase 2) enzym

5 In this study, we identified acid ceramidase (A-CDase) as a general transcription tar

6 The expression of acid ceramidase (AC) - a cysteine amidase that hydrolyse

7 Acid sphingomyelinase (Asm) and acid ceramidase (Ac) are parts of the sphingolipid metab

8 (e) whether physiological hypoxia increased acid ceramidase (AC) expression; and (f) the effect of t

9 In the present study, smooth muscle-specific acid ceramidase (Ac) gene knockout mice (Asah1(fl/fl)/SM

10 Lysosomal acid ceramidase (Ac) has been shown to be critical for c

11 Acid ceramidase (AC) hydrolyzes ceramides into sphingoid

12 Acid ceramidase (AC) hydrolyzes ceramides, which are cen

13 s regulating ulcerative colitis, the role of acid ceramidase (AC) in intestinal inflammation is yet t

14 omatic individuals (73%) exhibited increased acid ceramidase (AC) in their serum, measured by Western

15 Acid ceramidase (AC) is a cysteine hydrolase that plays

16 Acid ceramidase (AC) is a key regulatory enzyme involved

17 Acid ceramidase (AC) is a lysosomal cysteine amidase tha

18 Human acid ceramidase (AC) is a lysosomal cysteine amidase, wh

19 Acid ceramidase (AC) is a lysosomal cysteine hydrolase t

20 Acid ceramidase (AC) is a lysosomal hydrolase encoded by

21 Acid ceramidase (AC) is an intracellular cysteine amidas

22 Acid ceramidase (Ac) is part of the sphingolipid metabol

23 Acid ceramidase (AC) is the lysosomal enzyme that degrad

24 We hypothesized that Acid Ceramidase (AC) overexpression would counteract the

25 Autoproteolytic cleavage of the inactive acid ceramidase (AC) precursor into the active heterodim

26 In addition, the acid ceramidase (AC) was also upregulated by Ca2+(o); an

27 demonstrate that ceramide-deacylating enzyme acid ceramidase (AC) was preferentially upregulated in i

28 C12 myotubes that constitutively overexpress acid ceramidase (AC), an enzyme that catalyzes the lysos

29 ons revealed that desipramine down-regulated acid ceramidase (AC), but not sphingosine kinase, at the

30 The cysteine amidase, acid ceramidase (AC), hydrolyzes these substances into s

31 show that the ceramide-metabolizing enzyme, acid ceramidase (AC), is expressed in human cumulus cell

32 in the gene (Asah1) encoding one ceramidase, acid ceramidase (AC), lead to the lysosomal storage diso

33 s-dominant-negative (DN)-TGFB, DN-Wnt8a, and acid ceramidase (AC)-to induce CM-like cells.

34 h cDNA and genomic sequences encoding murine acid ceramidase (AC; E.C. 3.5.1.23) have been isolated a

35 Herein we report the mechanism of human acid ceramidase (AC; N-acylsphingosine deacylase) cleava

36 ns in the ASAH1 gene, resulting in deficient acid ceramidase (ACDase) activity.

37 osomal storage disorders caused by deficient acid ceramidase (ACDase) activity.

38 Interestingly, cells deficient in acid ceramidase (aCDase) also exhibited defects in CCL5

39 In MVBs, acid ceramidase (aCDase) converts ceramide into sphingos

40 We previously demonstrated that inhibiting acid ceramidase (aCDase) increases ceramide in HSCs to a

41 ugh the deacylation of galactosylceramide by acid ceramidase (ACDase).

42 cumulation of ceramide through inhibition of acid ceramidase (aCDase).

43 Acid ceramidase activities in skin fibroblasts and EBV-t

44 e detected, providing an accurate measure of acid ceramidase activity as low as 0.1 pmol/mg protein/h

45 phages revealed rapid internalization of the acid ceramidase activity from the hamster cell media but

46 s study reports a new assay method to detect acid ceramidase activity in vitro using Bodipy or lissam

47 be useful wherever the in vitro detection of acid ceramidase activity is of importance.

48 A deficiency of acid ceramidase activity results in the lipid storage di

49 ctate, perinuclear distribution, although no acid ceramidase activity was detected in the transfected

50 increasing ceramide production by inhibiting acid ceramidase activity was sufficient to upregulate ca

51 id, and highly sensitive method to determine acid ceramidase activity, and that it could be useful wh

52 Using mouse kidney extracts as the source of acid ceramidase activity, this new method was compared w

53 er disease patient to the same extent as the acid ceramidase activity.

54 Treatment with recombinant acid ceramidase ameliorates the two pivotal features of

55 a provide important new information on human acid ceramidase and further document its central role in

56 f p21 with UC2288 prevented the induction of acid ceramidase and inhibited both the formation of PGCC

57 data suggest that p21 functions upstream of acid ceramidase and plays an important role in polyploid

58 s no effect on transcriptional activation of acid ceramidase and that CerS2 slightly but significantl

59 These studies provide new insights into acid ceramidase and the related lipid hydrolase, acid sp

60 eramide degradation to increase ceramide via acid ceramidase (ARN082) for proof of principle.

61 se analysis of sequences homologous to human acid ceramidase (ASAH) revealed a 1233-bp cDNA (previous

62 Acid ceramidase (ASAH1) plays a pivotal role in regulati

63 CR analysis revealed that only expression of acid ceramidase (ASAH1) was increased.

64 Moreover, suppression of expression of acid ceramidase (ASAH1), an enzyme that produces SPH, in

65 ny formation depends on the lysosomal enzyme acid ceramidase (ASAH1).

66 we report detailed characterization of this acid ceramidase-associated "reverse activity" and provid

67 Blocking acid ceramidase but not sphingosine kinase activity in a

68 downregulation of IRF8 prevented changes of acid ceramidase, ceramide, and sphingosine in CF epithel

69 These results suggest that the acid ceramidase/ceramide signaling pathway controls EV r

70 rare autosomal recessive disorder caused by acid ceramidase deficiency that usually presents as earl

71 Acid ceramidase, expressed in a recombinant SV, decrease

72 8 (IRF8) and a concomitant downregulation of acid ceramidase expression with CF and an increase of ce

73 beta1-integrins downregulate acid ceramidase expression, resulting in further accumul

74 -(alpha) and 40 (beta)-kDa subunits as human acid ceramidase from natural sources, had an acidic pH o

75 fl)/Alb(cre) (liver-specific deletion of the acid ceramidase gene Asah1) mice.

76 or in cells, and it was a poor inhibitor of acid ceramidase (IC50>500 microM).

77 S6 can mediate transcriptional activation of acid ceramidase in a JNK-dependent manner that is indepe

78 es have demonstrated a downregulation of the acid ceramidase in CF epithelial cells resulting in an i

79 ) connects to a marked downregulation of the acid ceramidase in human and murine CF epithelial cells.

80 Loss of acid ceramidase in myeloid cells suppresses intestinal n

81 Overexpression of acid ceramidase in normal human skin fibroblasts also le

82 Our results implicate acid ceramidase in the pathogenesis of GBA1-associated P

83 Acid ceramidase inhibitor, ARN082, elevated cellular cer

84 ly demonstrated that the sphingolipid enzyme acid ceramidase is dispensable for polyploidization upon

85 Acid ceramidase is one of the key enzymes that regulate

86 ure (renamed human ASAHL since it is a human acid ceramidase-like sequence), chromosomal location, pr

87 macrophages have high constitutive levels of acid ceramidase mRNA, protein, and activity.

88 Acid ceramidase (murine gene code: Asah1) (50 kDa) belon

89 Acid ceramidase (N-acylsphingosine amidohydrolase) is th

90 effects of treatment with recombinant human acid ceramidase on inflammation and infection.Methods: S

91 Effects of treatment with recombinant human acid ceramidase on sphingolipid profile and inflammatory

92 morpholino-1-propanol (PDMP), which inhibits acid ceramidase or glucosylceramide synthase and then in

93 therapy stress promotes polyploidy, and that acid ceramidase plays a role in depolyploidization.

94 Using an acid ceramidase promoter driven luciferase reporter plas

95 y measurements to study the role of IRF8 for acid ceramidase regulation.

96 Inhibition of acid ceramidase, the lysosomal enzyme that deacylates Gl

97 s method utilizes purified human recombinant acid ceramidase to completely hydrolyze ceramide to sphi

98 Treatment with recombinant human acid ceramidase, to decrease ceramide, reduced both infl

99 this activity could be co-precipitated with acid ceramidase using anti-ceramidase antibodies.

100 t CFTR deficiency causes a downregulation of acid ceramidase via upregulation of IRF8, which is a cen

101 The increase in acid ceramidase was confirmed by expression and activity

102 tigated sphingolipid pathways and found that acid ceramidase was constitutively overexpressed in leuk

103 Human acid ceramidase was overexpressed in Chinese hamster ova

104 The high levels of acid ceramidase were specific to alveolar macrophages, b

105 ndent induction of p21 and that knockdown of acid ceramidase, which hydrolyzes ceramide, does not int