ライフサイエンスコーパス: actin filament

1 e edge oriented toward the barbed end of the actin filament.

2 hanochemistry of myosins that bind along the actin filament.

3 s, which subsequently spreads throughout the actin filament.

4 bilizes once it spreads throughout an entire actin filament.

5 inite range of mechanical coupling along the actin filament.

6 ip1 and attached to the end of the nucleated actin filament.

7 ultimately leading to full decoration of the actin filament.

8 strands, which lie on opposite sides of the actin filament.

9 ds on the cyclical interaction of myosin and actin filaments.

10 ciates with factors linked to centrosome and actin filaments.

11 dulins (Tmods), which cap the pointed end of actin filaments.

12 ead compound ATM-3507 with Tpm3.1-containing actin filaments.

13 nucleating, elongating, and bundling linear actin filaments.

14 ers and modestly accelerates the assembly of actin filaments.

15 ylation of Cdc8 weakens its interaction with actin filaments.

16 ization once cable formation is initiated on actin filaments.

17 eads in antiparallel networks of overlapping actin filaments.

18 reased occupancy, indicative of more bundled actin filaments.

19 en the cofilin-decorated and bare regions on actin filaments.

20 sliding of bipolar myosin II filaments along actin filaments.

21 with basal bodies, basal feet, rootlets, and actin filaments.

22 ed cell invasion increase via depolymerizing actin filaments.

23 ts that occurred more frequently for shorter actin filaments.

24 A key function of ADF/cofilin is to sever actin filaments.

25 o depends on the geometrical organization of actin filaments.

26 which requires forces generated by MYO7B and actin filaments.

27 ng the severing process of cofilin-decorated actin filaments.

28 transport, initiated by nearby elongation of actin filaments.

29 al network that contains abundant unbranched actin filaments.

30 r excitation waves propagating on bundles of actin filaments.

31 hort filaments emanate from existing, longer actin filaments.

32 shape but instead is associated with loss of actin filaments.

33 hed structures rather than linear unbranched actin filaments.

34 ctin binding protein key to the stability of actin filaments.

35 conserved proteins that cooperatively sever actin filaments.

36 ng and spreading cooperatively on individual actin filaments.

37 the branch junction and its interaction with actin filaments.

38 nts, suggesting that both proteins stabilize actin filaments.

39 between Ena/VASP proteins, EVH1 ligands, and actin filaments.

40 tor that promotes polymerization of branched actin filaments.

41 networks but does not associate with relaxed actin filaments.

42 erences in conformation compared with native actin filaments.

43 ous superhelical cables that wrap around the actin filaments.

44 plex only once it is bound to the side of an actin filament [5, 6].

45 structural changes are driven by changes in actin filament and microtubule dynamics and organisation

46 s: 1) polymerization and depolymerization of actin filaments and 2) remodeling of cross-linker-rich a

47 he cytoskeleton, which is mainly composed of actin filaments and actin-binding partners.

48 ally between connected pairs of antiparallel actin filaments and are oriented at an angle of about 25

49 The actin cytoskeleton, a dynamic network of actin filaments and associated F-actin-binding proteins,

50 The formation, maintenance, and turnover of actin filaments and bundles in the actin cortex are impo

51 between gelsolin-induced alterations in the actin filaments and changes due to myosin motor activity

52 In vivo, colocalization of actin filaments and divalent ions are suppressed, and ce

53 fibrillar structures such as microtubules or actin filaments and do not bind to amorphous aggregates.

54 The assembly of actin filaments and filament networks generate forces th

55 We find that SMTNL2 binds to actin filaments and is required to slow down the turnove

56 Hof1 bundles actin filaments and links them to septins in vitro.

57 ents showed that direct interactions between actin filaments and lipid bilayers are possible and that

58 Actin filaments and microtubules create diverse cellular

59 membrane macrodomains and subpopulations of actin filaments and microtubules.

60 common set of ancient proteins, principally actin filaments and myosin-II motors.

61 ions in regulating the formin Bnr1, binds to actin filaments and organizes actin cables in vivo.

62 Since CD2AP binds to both actin filaments and PI3K, CD2AP might bridge actin assem

63 CP) binds the rapidly growing barbed ends of actin filaments and prevents the addition (or loss) of s

64 t coronin accelerates the release of Pi from actin filaments and promotes highly cooperative cofilin

65 edicting synapsin functional binding to ATP, actin filaments and secretory vesicles.

66 We further show that LUZP1 localizes to both actin filaments and the centrosome/basal body.

67 The initial binding of tropomyosin onto actin filaments and then its polymerization into continu

68 This filament design is stiffer than the actin filament, and has likely been selected for during

69 lamellipodia, depends on the barbed ends of actin filaments, and requires both the LIM domain and th

70 ing to support the formation of more complex actin filament architectures-such as those mandated by e

71 Bundles of actin filaments are central to a large variety of cellul

72 Actin filaments are conductive to ionic currents, mechan

73 Actin filaments are cortical within the protrusion, as o

74 py, making it difficult to determine whether actin filaments are directly associated with specific me

75 hanics are well-captured by a model in which actin filaments are dynamically connected by a single do

76 recruit DIAPH1 to the plasma membrane where actin filaments are generated.

77 ized, the regulation and function of nuclear actin filaments are only recently emerging.

78 a, we used an in vitro motility assay, where actin filaments are propelled by surface-adsorbed heavy

79 nteractions of elastic titin with sarcomeric actin filaments are revealed.

80 al networks, and changes in microtubules and actin filaments are well studied.

81 rmation is Msp300/Nesprin-1, which organizes actin filaments around the new synapse.

82 t a 9.0 angstrom resolution structure of the actin filament Arp2/3 complex branch junction in cells u

83 tips; they drive retrograde extension of an actin filament array that specifies anterograde microtub

84 of structurally and biochemically different actin filament arrays.

85 ayed dramatically amplified flow of cortical actin filaments, as revealed by total internal reflectio

86 example, modulate cell shape by accelerating actin filament assembly locally and slowing filament cap

87 t evidence that N-Wasp, a protein regulating actin filament assembly through Arp2/3 complex-dependent

88 Thus, spatially constrained actin filament assembly utilizes an adaptive mechanism e

89 genetic suppression of mutants defective in actin filament assembly/stability at the division site.

90 Actin filaments associated with myosin motors constitute

91 to both barbed and pointed ends of a short F-actin filament at the anticipated locations for polymeri

92 e CaMKII holoenzymes cross-linked multiple F-actin filaments at random, whereas at higher CaMKII/F-ac

93 sistent with the emergence of highly aligned actin filaments at the cell cortex of the vegetal hemisp

94 itions, we can now visualize distinct linear actin filaments at the posterior of the nucleus in both

95 t an angle of about 25 degrees away from the actin filament axes.

96 uction, contraction of cardiac myocytes, and actin filament-based movement of cardiac cells.

97 Actin filaments became more 'organized' after IAA stoppe

98 Long actin filaments bend between attachment sites in the coa

99 of the tropomyosin cable that fits onto the actin filament between the tip of the myosin head and a

100 volves complex structural rearrangements and actin filament binding, which are yet to be understood.

101 cal forces contribute to disassembling "old" actin filament branches in cells.

102 vated Arp2/3 complex only nucleates branched actin filaments but means branched actin networks must b

103 AFL1 partially colocalized with actin filaments but not with microtubules, further indic

104 The dynamin GTPase is known to bundle actin filaments, but the underlying molecular mechanism

105 Formins direct the elongation of unbranched actin filaments by binding their barbed ends and process

106 acts MICAL1, an enzyme known to depolymerize actin filaments by direct oxidation.

107 Perturbation of actin filaments by either cytochalasin-D or conditional

108 rescence microscopy to ask how decoration of actin filaments by five biologically prominent Tropomyos

109 ein (WASP), and contraction of the resultant actin filaments by myosin II.

110 Rearrangement of actin filaments by polymerization, depolymerization, and

111 y suggest that polar and nematic patterns of actin filaments can interact and dynamically transform i

112 inase regulation of FSGS mutation binding to actin filaments can occur in cells was shown by phosphor

113 kinetics of CH1-CH2 domain mutants varies as actin filament conformation is altered by perturbations

114 membrane skeleton, which comprises ring-like actin filaments connected to each other by spectrin tetr

115 At pCa 3.7, actin filaments containing high-alanine TnT had an eleva

116 Here, we used EM to show that actin filaments convert directly into globular material.

117 e protrusions driven by polymerization of an actin filament core, can adhere to the extracellular mat

118 mined by the membrane skeleton, a network of actin filaments cross-linked by spectrin and attached to

119 catastrophic cofilin-dependent decreases in actin filament density, sudden decreases in traction for

120 and remodeled in a few seconds, yet in vitro actin filaments depolymerize slowly over minutes.

121 ated tracking of elongating and intersecting actin filaments, detection of loop formation and constri

122 etect and image individual membrane-attached actin filaments diffusing within the acto-myosin network

123 lation is required for CAP1 function in both actin filament disassembly and cell adhesion.

124 had defects in rescuing the reduced rate of actin filament disassembly in the CAP1 knockdown HeLa ce

125 Coronin and Aip1 promote cofilin-mediated actin filament disassembly, but the mechanism is somewha

126 cofilin family are the central regulators of actin filament disassembly.

127 tions required for cell morphogenesis, while actin-filament disassembly dynamics relax stress and fac

128 lso made FM4-64 uptake less sensitive to the actin filament disruptor Latrunculin B (LatB).

129 Arp2/3-nucleated actin filaments drive crawling motility and phagocytosis

130 We believe that the reorganization of actin filaments during EMT modified the PM structures, c

131 erved actin-regulating protein that enhances actin filament dynamics and also regulates adhesion in m

132 However, AFL1 did not substantially inhibit actin filament dynamics, indicating that AFL1 acts via a

133 n and the role of tropomyosins in regulating actin filament dynamics.

134 Actin filaments elongate and shorten much faster at thei

135 ly expressed in eukaryotes and that regulate actin filament elongation by binding to both monomeric a

136 blished a kinetic model of Ena/VASP-mediated actin filament elongation.

137 dependent biochemical activities of WAVE1 on actin filament elongation.

138 naling promotes the polymerization of linear actin filaments emanating from the INM towards the nucle

139 tor myosin V transports cargo by stepping on actin filaments, executing a random diffusive search for

140 c co-activation does not require preexisting actin filaments, explaining how Wsp1 contributes to acti

141 ate CaMKII, which leads to remodeling of the actin filament (F-actin) network in the spine.

142 el for the complex structure of CPEB3 and an actin filament (F-actin).

143 ever, it is unclear how interactions between actin filaments (F-actin) and associated proteins are me

144 Vt binds actin filaments (F-actin) and promotes vinculin dimeriza

145 yl cyclase activity of ExoY is stimulated by actin filaments (F-actin) and that ExoY alters actin cyt

146 Actin filaments (F-actin) are key components of sarcomer

147 In contrast, whether actin filaments (F-actin) are required for or are even p

148 ns including capping proteins that stabilize actin filaments (F-actin) by inhibiting actin polymeriza

149 ment of innovative tools for live imaging of actin filaments (F-actin) enabled the detection of surpr

150 the actin cortex, a thin network of dynamic actin filaments (F-actin) situated just beneath the plas

151 nown to depend on the cytoskeleton including actin filaments (F-actin), microtubules (MT), and interm

152 o ensembles that bind, slide, and cross-link actin filaments (F-actin).

153 hosphorylation-mediated release of Cdc8 from actin filaments facilitates access of the actin-severing

154 adaptation during filament bundle formation: actin filaments first align in the direction of the exte

155 ssociation of a single AIP1 molecule to/from actin filaments followed a second-order and a first-orde

156 at clathrin plaques and surrounding branched actin filaments form microdomains that anchor a three-di

157 xit is accompanied by a burst in cytoplasmic actin filament formation that depends on WASH and the Ar

158 lins bind to globular (G-)actin and regulate actin filament formation.

159 Networks of branched actin filaments formed by Arp2/3 complex generate and ex

160 e may be a primary mechanism of accelerating actin filament fragmentation by other actin-destabilizin

161 At the Z-band, actin filaments from adjoining sarcomeres overlap and ar

162 inhibitor SMIFH2, which causes detachment of actin filaments from formin molecules, produces similar

163 In striated muscles, Tmods prevent actin filaments from overgrowing, whereas in non-muscle

164 constitute assembly of mammalian, non-muscle actin filaments from physiological concentrations of pro

165 timulates the assembly of rapidly elongating actin filaments from PRF1-bound actin.

166 rks requires control over the speed at which actin filaments grow.

167 mmalian cells indeed operate at the limit to actin filament growth imposed by profilin and formins.

168 Arp2/3 complex nucleates branched actin filaments important for cellular motility, endocyt

169 he ends are reeled in by barbed end-anchored actin filaments in adjacent segments.

170 lpha-actinin in transmitting tension between actin filaments in adjoining sarcomeres.

171 s (Tpm) determine the functional capacity of actin filaments in an isoform-specific manner.

172 d crosslinks nonpolymerizing MT plus ends to actin filaments in axonal GCs, preventing MT depolymeriz

173 dings suggest that conformational changes of actin filaments in cells could help to direct accessory

174 live imaging for F-actin showed abundance of actin filaments in embryonic mesoderm only.

175 ly stiffen the cytoskeleton by sliding polar actin filaments in opposite directions.

176 6/7 filaments were more potent barriers than actin filaments in pausing MT growth and dissociating EB

177 eled tropomyosin isoform Tpm1.8 to unlabeled actin filaments in real time.

178 ch as polymerization and depolymerization of actin filaments in response to intracellular and extrace

179 lin is a giant protein that winds around the actin filaments in the skeletal muscle sarcomere.

180 tes in F-actin-rich domains of GCs and binds actin filaments in vitro.

181 grated into an isotropic network of cortical actin filaments in which filamin A (FlnA) localizes pref

182 of the cofilin/ADF family of proteins sever actin filaments, increasing the number of filament ends

183 that Tpm1.8 decorates the two strands of the actin filament independently.

184 other proteins regulate the assembly of the actin filaments into a contractile ring positioned betwe

185 The assembly of actin filaments into distinct cytoskeletal structures pl

186 erved proteins that non-covalently crosslink actin filaments into tight bundles.

187 The propagation of localisations on a single actin filament is experimentally unfeasible to control.

188 X-ray diffraction revealed that the actin filament is twisted with a larger radius, that tro

189 The severing of actin filaments is mainly achieved by cofilin, assisted

190 easts, although the assembly of two zones of actin filaments is specific for fission yeast and not es

191 ve quantitatively distinct abilities to tune actin filament length and turnover.

192 his hypothesis, ectopic AFL1 expression made actin filaments less sensitive to disruption by LatB or

193 drial respiration (rotenone) caused profound actin filament loss, blocked RhoA/ROCK signalling and re

194 ent of the cytoskeleton (microtubules: high; actin filaments: low) as the basis for intrinsic cell po

195 Without MYO7B or actin filaments, many clathrin-coated pits fail to be se

196 s suggest that reduced binding of cofilin to actin filaments may be the underlying cause of the obser

197 r (0.39-5.85 um, median 1.92 um) and contain actin filaments, microtubules, and cytokeratin 19-based

198 We developed a mesoscopic-length-scale actin filament model to investigate force-induced filame

199 d of directionally unaligned, densely packed actin filaments, most shorter than 150 nm.

200 These structures and the comprising actin filaments must be mechanically stable to sustain t

201 bind barbed ends and retain pointed ends of actin filaments near beads and we identified Spire's bar

202 the model shows that the architecture of an actin filament network does not perturb the total amount

203 orhabditis elegans embryo to explore how the actin filament network in the ring and the surrounding c

204 To define how actin filament network organization affects MyoVa cargo

205 The cell's dense 3D actin filament network presents numerous challenges to v

206 oviding direct structural evidence for rapid actin filament network turnover induced by GTPase signal

207 During autophagy, actin filament networks move and remodel cellular membra

208 protein (Arp)2/3 complex nucleates branched actin filament networks pivotal for cell migration, endo

209 chanical forces influence the disassembly of actin filament networks, specifically, the dissociation

210 Here we show that branched actin filament networks, the main pushing machinery in c

211 be changed by modification of the molecular actin filament nucleation and assembly rates.

212 eukaryotic formins in that it promotes both actin filament nucleation and elongation and competes wi

213 these observations on the kinetic pathway of actin filament nucleation and polymerization and possibi

214 d IQGAP1 robustly stimulates DIAPH1-mediated actin filament nucleation in vitro In contrast, the acti

215 odeling is the spatio-temporal regulation of actin filament nucleation.

216 The actin filament nucleator Arp2/3 complex is activated at

217 Arp2/3 complex, a crucial actin filament nucleator, undergoes structural rearrange

218 One family of actin filament nucleators, the Diaphanous-related formin

219 s, DNA movement and clustering are driven by actin filament nucleators.

220 modulated by interplay between the two major actin filament nucleators.

221 Our previous models predicted that actin filaments of the endocytic meshwork continually po

222 capacity, each dynamin helix captures 12-16 actin filaments on the outer rim of the helix.

223 cts of AFL1 on endocytosis may be related to actin filament organization or stability.

224 involved in biological functions relevant to actin filament organization, cytoskeleton biology, and c

225 ons related to endocytosis and regulation of actin filament organization, processes for which the eff

226 tively polarized the network by aligning the actin filament plus-ends.

227 filin synergize to processively depolymerize actin filament pointed ends at a rate 330-fold faster th

228 otors are influenced by changes in the local actin filament polarity alignment within the network.

229 We studied actin filament polymerization and nucleation with molecu

230 type-I myosins to produce force by promoting actin filament polymerization.

231 e complete description of Tpm1.8 kinetics on actin filaments presented here provides molecular insigh

232 s a central role in stabilizing cytoskeleton actin filaments, probably linked with tumor tissue remod

233 cial protein-protein interaction involved in actin filament processing and cell migration.

234 Interactions of myosin-II with actin filaments produce force to assemble and then const

235 nucleation to stimulate robust and localized actin filament production in vivo.

236 binding of a short cross-linker protein to 2 actin filaments promotes the binding of other short cros

237 ied to fluorescent labels attached to single actin filaments, provides precisions within tens of nano

238 A model based on polymerizing actin filaments pushing against mitochondria, thus gener

239 ave other defects, as NMII interactions with actin filaments regulate physiological processes such as

240 ut not with microtubules, further indicating actin-filament-related function of AFL1.

241 se data provide insight into endocytosis and actin filament responses to low water potential stress a

242 y altering interactions between semiflexible actin filaments, rigid microtubules, and crosslinking pr

243 Within both networks, we defined each actin filament's 3D spatial position using superresoluti

244 illi, each supported by a parallel bundle of actin filaments several microns in length.

245 ing is consistent with cooperativity between actin filament severing by myosin-induced forces and by

246 is known about the biophysical basis of the actin filament severing by these proteins.

247 r Ca(2+) concentration, appreciably enhanced actin filament severing caused by HMM-induced forces at

248 Cofilin is an actin filament severing protein necessary for fast actin

249 l origins of cooperative cofilin binding and actin filament severing.

250 liant substrates, the vinculin-bound ventral actin filaments shorten, resulting in short-range connec

251 LatB or Cytochalasin D and led to increased actin filament skewness and decreased occupancy, indicat

252 As a result of the RLC swapping, actin filament sliding velocity increased by ~10-fold fo

253 2/3 complex on the pointed ends of nucleated actin filaments, so Dip1 is consumed in the reaction.

254 ropomyosins, which in fission yeast controls actin filament stability and division site placement.

255 er effect could be partially mimicked by the actin filament stabilizer Jasplakinolide (JASP).

256 ells have diverse protrusive and contractile actin filament structures, which compete with one anothe

257 t a biophysical model for dynamic bundles of actin filaments submitted to an external load.

258 lin binding and allow tropomyosin binding to actin filaments, suggesting that both proteins stabilize

259 Leaks are prevented by contractile actin filaments surrounding the diapedesis pore, keeping

260 ll migration entails networks and bundles of actin filaments termed lamellipodia and microspikes or f

261 5E that demonstrated even greater binding to actin filaments than K255E and the other FSGS mutants.

262 forming long chains along the two strands of actin filaments that act as gatekeepers for the binding

263 geting drugs suggest that PMS contains short actin filaments that are depolymerization resistant and

264 ractions between myosin II motor domains and actin filaments that are powered by turnover of ATP unde

265 y associate with a criss-cross array of long actin filaments that comprise part of this interstitial

266 rity in emerin, and thereby controls nuclear actin filaments that spatially segregate viral DNA from

267 By applying force to actin filaments, the plasma membrane, and intracellular

268 s comprised of randomly oriented, unbranched actin filaments; the other was comprised of Arp2/3-branc

269 owever, myosin motor activity also fragments actin filaments through motor-induced forces, suggesting

270 Binding of RePRP reduces the abundance of actin filaments, thus diminishing noncellulosic polysacc

271 bles into polymeric filaments, which pull on actin filaments to generate force and motion.

272 intain row identity, control addition of new actin filaments to increase stereocilia diameter, and co

273 disruption to assign specific populations of actin filaments to individual formins.

274 activates Arp2/3 complex without preexisting actin filaments to nucleate 'seed' filaments that activa

275 plex (WRC) promotes the required assembly of actin filaments to push the front of the cell ahead.

276 hat neither talin nor vinculin alone recruit actin filaments to the membrane.

277 sion originates from barbed-end anchoring of actin filaments to the plasma membrane, providing resist

278 or cytoskeletal components, microtubules and actin filaments, together with a microtubule motor, kine

279 n motors from thick filaments pulling nearby actin filaments toward the sarcomere centre.

280 region approached Cys-190 of tropomyosin as actin filaments transitioned to the inactive B state; th

281 the "focal adhesion clutch," in which moving actin filaments transmit force to integrins via dynamic

282 ation is required for CAP1 functions in both actin filament turnover and adhesion, and the novel mech

283 er cell invasiveness through effects on both actin filament turnover and cell adhesion.

284 ability of interacting with microtubules and actin filaments unique to mammalian melanophilin or did

285 on on the microsecond rotational dynamics of actin filaments, we attached a phosphorescent probe to F

286 In vitro, UNC-87 bundled actin filaments, whereas CLIK-1 bound to actin filaments

287 red to ErbB3 was abolished by depolymerizing actin filaments, whereas ErbB2 expression induced a subs

288 ranes to protrude and reattach across a gap, actin filaments, which are relatively weak as single fil

289 ; the other was comprised of Arp2/3-branched actin filaments, which effectively polarized the network

290 ular protrusions containing microtubules and actin filaments, which respectively drive McTN extension

291 th clathrin plaques and surrounding branched actin filaments, while CNM-causing mutations lead to des

292 wards the opposite end of the microtubule or actin filament with respect to the rest of the motor fam

293 from both the barbed and pointed ends of the actin filament with similar fast kinetics of 10 to 15 su

294 electron microscopy (cryo-EM) to reconstruct actin filaments with bound AMPPNP (beta,gamma-imidoadeno

295 We found that ExoY can directly bundle actin filaments with high affinity, comparable with euka

296 ution cryo-electron microscopy structures of actin filaments with N-1-pyrene conjugated to cysteine 3

297 Cofilin binds actin filaments with positive cooperativity, forming clu

298 ia polarity, and altered the organization of actin filaments within lamellipodia.

299 firmed this localization by directly imaging actin filaments within the native cellular environment.

300 led actin filaments, whereas CLIK-1 bound to actin filaments without bundling them and antagonized UN