ライフサイエンスコーパス: active enzyme

1 id binding domain, produced a constitutively active enzyme.

2 inucleotide (FAD), which was not the part of active enzyme.

3 s a new limiting factor in the production of active enzyme.

4 o mammalian aconitase (mACO2) to generate an active enzyme.

5 e oxidase-molybdenum domain yielding a fully active enzyme.

6 rap the fluorochromes in the vicinity of the active enzyme.

7 +) and calmodulin-independent constitutively active enzyme.

8 mitting heme binding and dimerization to the active enzyme.

9 eletions of 1-24 amino acids produce a fully active enzyme.

10 ing individual steps in its conversion to an active enzyme.

11 % of the rate of formation to regenerate the active enzyme.

12 expressed in Escherichia coli and yielded an active enzyme.

13 ed nine orthologues of known subunits but no active enzyme.

14 transforms the zymogen into a catalytically active enzyme.

15 TPase proteins associate preferentially with active enzyme.

16 so has sensitivity to detect up to 2.5 ng of active enzyme.

17 ptide complementation to produce a partially active enzyme.

18 Zn(2+), Co(2+), Ni(2+), and Cd(2+)-generated active enzyme.

19 e that undergoes autoprocessing to become an active enzyme.

20 ays be efficiently reduced to regenerate the active enzyme.

21 MP proenzyme noticeably exceeded that of the active enzyme.

22 d its cleavage at D175 no longer leads to an active enzyme.

23 active form, whilst ScKAT is the reduced and active enzyme.

24 nsform the nascent peptide into a stable and active enzyme.

25 n and subsequent generation of catalytically active enzyme.

26 CYP2F1 is an active enzyme.

27 nt temperature of the reduced, catalytically active enzyme.

28 r to turn only a fraction of a degree in the active enzyme.

29 locluster composition of HydG that comprises active enzyme.

30 (TERT), minimized hTR assembled biologically active enzyme.

31 rsity and relative abundance of carbohydrate-active enzymes.

32 thrin with either inorganic nanoparticles or active enzymes.

33 n observed for the protein scaffold of redox active enzymes.

34 sts characterizing functionally carbohydrate-active enzymes.

35 gulare genome revealed over 280 carbohydrate-active enzymes.

36 wever, once stabilized, they are efficiently active enzymes.

37 ported before for this class of carbohydrate-active enzymes.

38 mosomal duplication and encode catalytically active enzymes.

39 furthers progress toward reliably producing active enzymes.

40 ial for the characterization of carbohydrate-active enzymes.

41 that contributes to the ATP binding site in active enzymes.

42 oughout numerous C. perfringens carbohydrate-active enzymes.

43 , indicating the expression of catalytically active enzymes.

44 dentified in the P trichocarpa genome encode active enzymes.

45 of membrane physical properties on membrane-active enzymes.

46 expansion of its repertoire of carbohydrate-active enzymes.

47 mational fluctuations of single residues for active enzymes.

48 resource for discovering novel carbohydrate active enzymes.

49 resource for discovering novel carbohydrate active enzymes.

50 exon skipping and inclusion isoforms encode active enzymes.

51 g a wide variety of coexpressed carbohydrate-active enzymes.

52 n of an extensive repertoire of carbohydrate-active enzymes.

53 sequestration, and a number of carbohydrate-active enzymes.

54 All active enzymes accepted scoulerine and tetrahydrocolumba

55 ed by direct chromatographic isolation of an active enzyme-acetyl species.

56 ) that is proteolytically processed into the active enzyme after exposure to low pH in vitro or targe

57 Article describes a strategy for quantifying active enzyme analytes in a paper-based device by measur

58 that translocates to the cell surface as an active enzyme and co-localizes with cell surface annexin

59 se lead to significantly decreased levels of active enzyme and could be causal in the development of

60 2A subunits that prevent dissociation of the active enzyme and highlight inherent mechanisms of PP2A

61 nd more focused annotations for carbohydrate-active enzymes and antibiotic resistance genes.

62 ome sequence reveals a suite of carbohydrate-active enzymes and demonstrates a level of diversity at

63 ctions, which are widespread in carbohydrate-active enzymes and have long been associated with proces

64 i OMVs are conduits of signalling molecules, active enzymes and other proteins to its environment.

65 Transcript abundance of several carbohydrate-active enzymes and phenylalanine ammonia-lyases was also

66 e genes, such as those encoding carbohydrate-active enzymes and proteases, can be predicted from bact

67 quence reveals a diverse set of carbohydrate-active enzymes and provides further insight into lignoce

68 nd thiamine, and genes encoding carbohydrate active enzymes and secondary metabolism enzymes.

69 i possessed substantially fewer carbohydrate-active enzymes and secreted proteins than closely relate

70 succinogenes are enriched with carbohydrate-active enzymes and that intact OMVs were able to depolym

71 bably because they are shorter than those in active enzymes and they lack an amino acid that contribu

72 ed to estimate the relative concentration of active enzymes and variation in response to environmenta

73 conversion of the latent proenzyme into the active enzyme, and also via inhibition by tissue inhibit

74 beta building block can be assembled into an active enzyme, and provides further insight into the mol

75 e of one kinase molecule, which serves as an active enzyme, and specific docking sites on the C-lobe

76 n of substrate specificities of carbohydrate-active enzymes, and further explored using defined oligo

77 with an H69E mutation (PR(H69E)) folds into active enzymes, and it does so with an apparent Kd (diss

78 MYBs and identify transporters, carbohydrate-active enzymes, and small peptides as candidate molecula

79 , N106Q and K170R, resulted in catalytically active enzymes, and these active mutant enzymes gave pH-

80 beyond glycoside hydrolase and carbohydrate active enzymes, and to include a newly identified functi

81 imary and secondary metabolism, carbohydrate-active enzymes, and transporters, probably reflecting th

82 Our results provide insights into active enzyme architecture, explain biological variation

83 As sufficiently active enzymes are currently unknown, we synthesized twe

84 Many T. reesei genes encoding carbohydrate-active enzymes are distributed nonrandomly in clusters t

85 hat attack the OP-AChE conjugate to free the active enzyme, are inefficient.

86 1 mRNA led to a full-length, Pyl-containing, active enzyme as determined by immunoblotting, mass spec

87 color but has the same molecular mass as the active enzyme as determined by mass spectrometry.

88 as a model, we show that FAS foci represent active enzyme assemblies.

89 lenge, we introduce a strategy that takes an active enzyme (assuming that its activity is close to th

90 alytic processing in maintaining the pool of active enzyme at the cell surface.

91 MP endocytosis resulting in higher levels of active enzyme at the cell surface.

92 diverse probe and protein families to detect active enzymes at scale and resolution out of reach with

93 The ATCC 14579 genome encodes an active enzyme Bce14579I (GCWGC).

94 This regulation requires the presence of active enzyme, because mutated forms of heparanase lacki

95 Analysis of the purified, active enzyme by inductively coupled plasma-emission spe

96 t has been previously reported, but released active enzyme can also modulate cytokine expression, whi

97 PqqE isolated anaerobically yields an active enzyme capable of cleaving SAM to methionine and

98 MGL46, homodimeric recombinant HMGL35 is the active enzyme catalyzing acetyl-CoA and acetoacetate syn

99 In the Carbohydrate-Active Enzyme (CAZy) database, glycoside hydrolase famil

100 us and sulfur) transporters and Carbohydrate-Active enZyme (CAZy) genes was detected toward the botto

101 Based on the Carbohydrate-Active enZymes (CAZy) database, the genome of the refere

102 ion and colonization, including carbohydrate-active enzymes (CAZymes) and necrosis inducing effectors

103 combination of 200 pre-selected Carbohydrate-Active enzymes (CAZymes) and sulfatases were tested, ind

104 Carbohydrate-active enzymes (CAZymes) are extremely important to bioe

105 Carbohydrate-Active enZymes (CAZymes) are involved in the synthesis,

106 Carbohydrate-active enzymes (CAZymes) are very important to the biote

107 We describe here analysis of Carbohydrate Active Enzymes (CAZymes) from 3.5 gigabase sequences of

108 the growing number of predicted carbohydrate-active enzymes (CAZymes) has not been accompanied by a s

109 o identify reaction products of carbohydrate active enzymes (CAZymes) of the filamentous fungus Asper

110 as a promising source of novel carbohydrate active enzymes (CAZymes) that modify plant cell wall pol

111 ins encode a narrow spectrum of carbohydrate active enzymes (CAZymes) that reflect extreme specializa

112 Expression of the encoded carbohydrate-active enzymes (CAZymes) was then analyzed by metatransc

113 ll secreted proteins (SSPs) and carbohydrate-active enzymes (CAZymes) were the two major classes of e

114 arides possess large numbers of carbohydrate active enzymes (CAZymes), many of which have been catego

115 encoding a large contingent of Carbohydrate-Active enZymes (CAZymes), many of whose specific functio

116 rain, which were overwhelmingly carbohydrate active enzymes (CAZymes), with 95 large fungal scaffoldi

117 oir of microbial genes encoding carbohydrate-active enzymes (CAZymes).

118 This was also the case for carbohydrate-active enzymes (CAZymes).

119 bors an impressive reservoir of carbohydrate active enzymes (CAZymes).

120 merous candidate peptidases and carbohydrate-active enzymes ('CAZymes') (9) targeted for secretion.

121 and NF-kappaB activation by modulating redox-active enzymes, cell migration, phagocytosis, and bioene

122 o build phylogenies for CAZyme (carbohydrate active enzyme) classes and Pfam clans, which attested it

123 Redox-active enzyme cofactors derived from ribonucleotides hav

124 date the use of conditional expression of an active enzyme combined with phosphoproteomics to deciphe

125 Analysis of the carbohydrate active enzyme complement suggests an ability to utilize

126 ed to the membrane and recruits WbdA into an active enzyme complex by protein-protein interactions.

127 d POMT2, two of the causative genes, form an active enzyme complex in the posttranslational biosynthe

128 with its maturation factor DUOXA1 to form an active enzyme complex.

129 We found that NSI self assembles into highly active enzyme complexes and that high concentrations of

130 ceptor complexes, where they produce a fully active enzyme composed of two catalytic domains.

131 logic concentrations decreases the amount of active enzyme concentrations.

132 the mechanism by which the final key-lock or active enzyme conformation is achieved upon formation of

133 tional dual Src/Abl inhibitors targeting the active enzyme conformation, these inhibitors bind to the

134 troscopy, have been aimed at elucidating the active enzyme conformation.

135 ces of the nanocage enhance the stability of active enzyme conformations through the action of a stro

136 , features associated with the catalytically active, enzyme-coordinated copper center were evident be

137 The obtained active enzyme CrHydA1(adt) shows the same redox states i

138 filing of some members from the Carbohydrate-Active Enzymes database glycosyl transferase families GT

139 fore, are not yet assigned to a Carbohydrate-Active Enzymes database GT family.

140 The Carbohydrate Active Enzymes Database indicates that Lam81A belongs to

141 nsferase family, GT97, in CAZy (Carbohydrate-Active enZYmes Database).

142 osyltransferase families in the Carbohydrate-Active Enzymes database.

143 The ThYme (Thioester-active enzYme) database has been constructed to bring to

144 of E. granulatus is reduced in carbohydrate-active enzymes, despite a large expansion in genome size

145 , providing support for a model proposing an active enzyme dimer.

146 that a PS1/Pen2/Aph1a trimeric complex is an active enzyme, displaying biochemical properties similar

147 An active enzyme dynamic therapy by magnetically stimulated

148 of the O(2)-tolerance mechanism regenerates active enzyme, effectively completing a competitive four

149 e backside of the membrane has access to the active enzyme embedded in the electrode.

150 Like all redox-active enzymes, Erv1 requires one or more electron accep

151 ponent, i.e., highly purified, catalytically active enzyme expressed in a baculovirus system.

152 to detect and analyze the activity of redox active enzymes expressed on the surface of E. coli.

153 origins and activities of other carbohydrate active enzyme families and the biological significance o

154 To obtain a large quantity of active enzyme for substrate screening, we overexpressed

155 ased yields provide sufficient quantities of active enzyme for use in nuclear magnetic resonance (NMR

156 drate esterases, as well as accessory, redox-active enzymes for lignin depolymerization.

157 ructs were explored to identify an optimally active enzyme form for inhibition and structural studies

158 ite which is present in the glucose-induced, active enzyme form of GK and absent in the inactive apoe

159 ate stabilize an active tetramer over a less active enzyme form of mass comparable with that of a dim

160 on of proprotein convertase 2 (proPC2) to an active enzyme form; this action is accomplished via its

161 Active enzymes form distinctively shaped dimers, with a

162 These mutations do not prevent active enzyme formation; instead, they predominantly alt

163 ion of up to 90 mg of homogeneous and highly active enzyme from 10 g of cell material.

164 loci and comprises 19 different carbohydrate-active enzymes from different families, including a CNG-

165 otal amount of BChE (including inhibited and active enzyme) from 70 muLpost-exposure human plasma sam

166 Four catalytically active enzymes function in mice as DNA methyltransferase

167 When converted to the active enzyme, FXIa, all the monomeric mutants activated

168 iary Activity 5 (AA5) family of Carbohydrate-Active Enzymes (Gal/glyoxal oxidases) and is secreted to

169 s, including the acquisition of a putatively active enzyme, give the mitoribosome a distinct architec

170 ad to the inactivation of the constitutively active enzyme, glycogen synthase kinase-3 (GSK3), which

171 oires of two different types of carbohydrate-active enzymes, glycoside hydrolases and glycosyltransfe

172 ases (GALTs) are members of the carbohydrate-active enzyme glycosyltransferase (GT) 31 family (CAZy G

173 Carbohydrate-active enzyme glycosyltransferase family 8 (GT8) include

174 Indeed, the lyase-active enzyme has 3 orders of magnitude higher affinity

175 erest, but other types of proteins including active enzymes have also been expressed.

176 Carbohydrate-active enzymes have multiple biological roles and indust

177 anisms, suggesting that these day- and night-active enzymes have undergone thermal adaptation.

178 We uncover a complex set of carbohydrate-active enzymes, identify the genes required for the meth

179 of chelating resin, generating catalytically active enzyme in a process that appeared to involve dire

180 ctly to the Psi-synthase to form the minimal active enzyme in archaea.

181 P4H1 can be met by forced expression of the active enzyme in either pre-stalk (anterior) or pre-spor

182 human COT kinase that yields pure and highly active enzyme in sufficient yields for biochemical and s

183 While we did detect active enzyme in the brain following peripheral administ

184 propeptide lead to the secretion of a fully active enzyme in the cytosol of host cells independent o

185 ferase impair retention of the catalytically active enzyme in the Golgi complex.

186 The percentage of active enzyme in the preparations was also comparable, a

187 produced in Escherichia coli as soluble and active enzyme in the presence of its natural inhibitor a

188 scort the transition of MASP from zymogen to active enzyme in the PRM/MASP complex; rather, clusterin

189 ssed Nox4 represents the only constitutively active enzyme in this family, analysis of structural det

190 ve expression of genes encoding carbohydrate-active enzymes in a spatial and temporal pattern suffici

191 der of magnitude larger than the average for active enzymes in clades 1 and 2.

192 Using PyABPs, we were able to profile active enzymes in rat liver microsomes and identify pyre

193 nd the presence of metabolites, proteins and active enzymes in the watery saliva that could be involv

194 i.e., mutations preventing the generation of active enzyme) in MLII and with mild mutations (i.e., mu

195 (i.e., mutations allowing the generation of active enzyme) in MLIIIA.

196 ber of large and highly modular carbohydrate-active enzymes, including the mu-toxin and sialidases, w

197 slation with correctly acylated aa-tRNA) and active enzyme indistinguishable from the wild-type prote

198 We demonstrate conversion of constitutively active enzymes into peptide-operated synthetic allosteri

199 The active enzyme is a 1:1 complex of two homodimeric subuni

200 es that the monomer is inactive and that the active enzyme is at least a dimer.

201 teases mature from an inactive zymogen to an active enzyme is expected to inform new strategies for b

202 control of SBT3 activity, to ensure that the active enzyme is not released before it reaches the acid

203 ble universal fingerprint shared by all cold-active enzymes is a reduction of the activation enthalpy

204 osynthetic gene clusters and antibacterially active enzymes is discussed here.

205 ary model wherein the appearance of the most active enzyme isoform, glutaminase C (GAC), which is exp

206 tion converts the enzyme to a constitutively active enzyme, it results in PKU because of the decrease

207 ables multiplexed "writing" and "reading" of active enzyme levels across multiple protein families di

208 means to study the presence and location of active enzyme levels in different cell types, such as he

209 esses family-wide chemical probes to convert active enzyme levels into amplifiable barcoded oligonucl

210 The active enzyme loading levels were tracked using an activ

211 flammatory factors, release of metabolically active enzymes, loss of proinflammatory cytokine product

212 lular secretion and proteolysis, derives the active enzyme (Lox) and the propeptide (Lox-PP).

213 rsor Pro-LOX that is processed to the 32-kDa active enzyme (LOX) and to an 18-kDa propeptide (LOX-PP)

214 Concentrations of active enzymes (matrix metalloproteinase [MMP]-8, elasta

215 yme F430, which is in the Ni(I) state in the active enzyme (MCRred1).

216 eductase (PFOR), suggesting that other redox active enzymes mediate this reaction.

217 the model suggests that the vast majority of active enzyme molecules in a population should be in the

218 romoter control resulted in self-assembling, active enzyme multimers.

219 , undergo alternative splicing, resulting in active enzymes named isoforms 1 (i1s) and novel truncate

220 tility and effectiveness of the carbohydrate-active enzymes of T. reesei, its genome encodes fewer ce

221 nic biotransformation, suggesting that other active enzymes of the AD process are also involved in OM

222 acteria produce a wide range of carbohydrate-active enzymes, of which glycoside hydrolases are the ma

223 ing uniform and reproducible coatings of the active enzyme on the miniature transducer element.

224 ut microbiome--which encode the carbohydrate active enzymes, or CAZymes, that are absent from the hum

225 In addition to the expected carbohydrate active enzymes, our new method reveals a large number of

226 enefit from the efficient wiring of a highly active enzyme pair and allow for the reversible conversi

227 In the active enzyme, phosphate release from the beta(E)-subuni

228 The membrane-active enzyme phospholipase D (PLD) catalyzes the hydrol

229 ogen bound to ErpP could be converted to the active enzyme, plasmin.

230 namics of the denitrifier population and the active enzyme pool, which controlled the rate function.

231 RNAP I suggest that HelD might also modulate active enzyme pools in response to cellular cues.

232 c methods can report directly on endogenous, active enzyme populations, which can differ greatly from

233 ent including the repertoire of carbohydrate active enzymes present within and between twins (e.g., p

234 ese data provide evidence for a biologically active enzyme product 7alpha-hydroxyPREG and suggests th

235 LOX has tumor-suppressor activity, while the active enzyme promotes metastasis.

236 This strategy, combined with active enzyme, provides a new chemoenzymatic route to th

237 , but decrease the fraction of catalytically active enzyme recovered in the limit of long time.

238 bstrate accessibility and complementation by active enzymes retargeted to a companion organelle.

239 liary cell components, but rapid assembly of active enzyme seemingly is assisted in the cell.

240 Carbohydrate-active enzymes such as glycoside hydrolases (GHs) and gl

241 ymes are also found among these carbohydrate-active enzymes, such as chitinases, chitobiases, and lyt

242 Active enzymes, such as proteases, often serve as valuab

243 ul for predicting mechanisms for other redox-active enzymes, such as the one for photosystem II, but

244 Analyses of carbohydrate active enzymes suggest that this fungus is a biotroph wi

245 the three beta subunit isoforms and form an active enzyme, suggesting the absence of selective alpha

246 ended polypeptides are putative carbohydrate-active enzymes, suggesting a potential role in carbohydr

247 The 2.66-A crystal structure of the most active enzyme suggests that the engineered active site c

248 ional bioinorganic hybrid nanostructures via active enzyme-templated synthesis of inorganic nanomater

249 ising allosteric mechanism that destabilizes active enzyme tetramer.

250 Its genome encoded more carbohydrate active enzymes than any previously sequenced member of t

251 yet to be demonstrated, and whether it is an active enzyme that directly methylates substrate(s) in v

252 Mutation of Asp101 results in an active enzyme that incorporates metal in vivo but shows

253 uinolone-resistant A. thaliana gyrase yields active enzyme that is resistant to ciprofloxacin.

254 study, the PL2 domain was found to encode an active enzyme that mediates efficient processing of nsp2

255 Several cell wall enzymes are carbohydrate active enzymes that contain a putative Carbohydrate Bind

256 dation involves the activity of carbohydrate-active enzymes that have previously been implicated in t

257 es constitute a ubiquitous class of membrane-active enzymes that play a key role in cellular signalin

258 plasma membrane is re-engineered to display active enzymes that promote extracellular matrix protein

259 ino acid residues 400-415) are catalytically active enzymes that retain the 5' to 3' scanning ability

260 common protein motifs found in carbohydrate-active enzymes that synthesize or depolymerize polysacch

261 interactions are present in all carbohydrate-active enzymes, these results have implications for unde

262 undance of our genomes, and the carbohydrate active enzymes they produce, between our chicken groups

263 n inert polypeptide is converted to a mature active enzyme through post-translational modifications.

264 nzymes that stimulate the function of weakly active enzymes through complex formation.

265 ramatically stimulate the function of weakly active enzymes through complex formation.

266 ro-protein cleavage and the formation of the active enzyme, thus leading to loss-of-function of ADAM1

267 helix, allowing the catalytic domain of the active enzyme to cleave the collagen alpha chains sequen

268 that can hetero-oligomerize with and target active enzyme to membranes.

269 oy invasive growth and powerful carbohydrate active enzymes to reduce multicellular plant tissues to

270 iochemical descriptions of the 'active-to-de-active' enzyme transition that occurs during hypoxia.

271 duck hepatitis B virus polymerase employing active enzyme translated in vitro and derived from intra

272 general base), and the mechanism of the most active enzyme variant, were characterized.

273 identification of clones encoding unusually active enzyme variants.

274 te for the conversion of the zymogen form to active enzyme was also identified between R275 and I276,

275 The active enzyme was isolated and identified as Arg-gingipa

276 MT1-MMP mRNA and active enzyme was quantified in placentas from FGR and a

277 on during leukocyte phagocytosis to form the active enzyme were well described, leading to the identi

278 me frames during which a broader spectrum of active enzymes were detected compared to initial timepoi

279 glycoside hydrolases and other carbohydrate active enzymes were known to reside within the celluloso

280 and nearby interface residues, catalytically active enzymes were recoverable for approximately 70% of

281 ography showed there were reduced amounts of active enzymes when co-incubated, indicating that cannib

282 generated against the soluble, catalytically active enzyme, whereas Abs reactive with cell surface-as

283 peptide bond is the key to formation of the active enzyme, which involves increased dynamics of the

284 ition, we decipher the process leading to an active enzyme, which involves the activation of the apo-

285 st that glycosylated linkers in carbohydrate-active enzymes, which are intrinsically disordered prote

286 E. weberi genome is depleted in carbohydrate active enzymes, which is consistent with reliance on a h

287 mes was established, with GAC being the most active enzyme while forming the longest structures.

288 The need to immobilize active enzyme, while ensuring high rates of substrate tu

289 of the [FeFe] hydrogenases by assembling the active enzyme with a version of the active site synthesi

290 Mutation of Thr162 to Ala results in an active enzyme with no cooperativity.

291 idyl peptidase I, storage, and release as an active enzyme with the three active proteases.

292 hanism involves selective association of the active enzyme with transcriptionally active nucleoids an

293 The deletion mutants produce correct-sized, active enzymes with a good correlation between enzyme am

294 genes encode almost identical, catalytically active enzymes with distinctive N-terminal peptide seque

295 TPSL genes from the genus Metarhizium encode active enzymes with sesquiterpene synthase activities of

296 ion mutant) is recovered as a constitutively active enzyme, with high basal autocatalytic activity an

297 proach designed to characterize and identify active enzymes within complex biological samples.

298 trate efforts to design and engineer stable, active enzymes without laborious high-throughput activit

299 olar to nanomolar concentrations of membrane-active enzymes without requiring labeled substrates or p

300 converted nitrosylated GAPDH to the reduced, active enzyme, without forming any glutathionylated GAPD