ライフサイエンスコーパス: adenylylation

1 off-pathway states incompatible with lysine adenylylation.

2 ed active site residues essential for ligase adenylylation.

3 trogen-excess conditions due to excessive GS adenylylation.

4 397 to Phe, Ala, or Ser was found to prevent adenylylation.

5 induce changes previously thought to require adenylylation.

6 onounced lag phase in the progress of target adenylylation.

7 he hydroxyl group to AMP in a process termed adenylylation.

8 y is inversely proportional to the extent of adenylylation.

9 eleased after formation, and then rebind for adenylylation.

10 racteristics similar to the form obtained by adenylylation.

11 d associated with the phosphocholination and adenylylation activities of the enzymes AnkX and DrrA/Si

12 ylylated by the UTase/UR and to activate the adenylylation activity of ATase.

13 the NRII phosphatase activity and the ATase adenylylation activity.

14 volves three distinct chemical steps: enzyme adenylylation, adenylyl transfer to DNA, and nick sealin

15 of a post-translational modification termed adenylylation/AMPylation in regulating signal transducti

16 ates the activity of glutamine synthetase by adenylylation and deadenylylation in response to signals

17 ranferase (ATase, EC 2.7.7.49) catalyzes the adenylylation and deadenylylation of glutamine synthetas

18 The adenylylation and deadenylylation of GS are catalyzed by

19 glutamine synthetase (GS) by catalyzing the adenylylation and deadenylylation of GS in response to s

20 Escherichia coli is regulated by the cyclic adenylylation and deadenylylation of Tyr-397 in each of

21 lysis revealed distinct requirements for the adenylylation and end-sealing reactions catalyzed by Lig

22 P. horikoshii ligase catalyzes auto-adenylylation and nick sealing in the presence of a diva

23 onal effects on the isolated steps of ligase adenylylation and phosphodiester bond formation.

24 of mutational effects on the isolated ligase adenylylation and phosphodiester formation reactions rev

25 -285 and Phe-286 in the catalysis of the DNA adenylylation and phosphodiester synthesis reactions.

26 The rates of DNA adenylylation and phosphodiester synthesis respond diffe

27 Examples of modification by N-acetylation, adenylylation and proteolytic processing were characteri

28 ling-defective mutants were active in ligase adenylylation and sealing a preadenylylated nick, thereb

29 y the antibiotic, including phosphorylation, adenylylation, and acetylation.

30 example of a PII protein that is modified by adenylylation, and demonstrates that this reaction is pe

31 ligation pathway (ligase adenylylation, DNA adenylylation, and phosphodiester synthesis).

32 the deadenylylation of GS was to inhibit GS adenylylation, and this was due to the allosteric regula

33 f the nick 3' nucleoside for catalysis of 5' adenylylation; and (ii) EcoLigA's potential to embed mut

34 characterized the kinetics of DrrA-catalyzed adenylylation as well as SidD-catalyzed deadenylylation

35 Co-immunoprecipitation and adenylylation assays demonstrated that this associated f

36 Interestingly, in contrast to adenylylation by DrrA, AnkX can covalently modify inacti

37 lytic activity due to covalent modification (adenylylation by GS adenylyltransferase).

38 which effectively inhibits catalysis in the adenylylation cycle (cycle II).

39 The order for the adenylylation cycle was ATP(in), FMN(in), pyrophosphate(

40 ponent steps of the ligation pathway (ligase adenylylation, DNA adenylylation, and phosphodiester syn

41 in light of available structural data on the adenylylation domains of ATP- and NAD-dependent ligases.

42 um suggested a two-metal mechanism of lysine adenylylation driven by a catalytic Mg(2+) that engages

43 We found that adenylylation enhances BiP's ATPase activity, which is r

44 This adenylylation event inactivates Rho GTPases by preventin

45 domains of IbpA catalyze a unique reversible adenylylation event that uses ATP to add an adenosine mo

46 The mechanistic insights to lysine adenylylation gained from the NgrRnl structures are like

47 coli glutamine synthetase (GS) by reversible adenylylation has provided one of the classical paradigm

48 AMPylation (adenylylation) has been recognized as an important post-

49 e the first functional data for Fic-mediated adenylylation in mammalian signaling.

50 of proteins that undergo phosphorylation or adenylylation in signal transduction cascades might be s

51 and suggests the intriguing possibility that adenylylation in the pathogenic versus non-pathogenic my

52 ting conditions, whereas there was little GS adenylylation in wild-type strains.

53 scherichia coli and is subject to reversible adenylylation (inactivation) by a bifunctional GS adenyl

54 tions and a rapid decrease of GS activity by adenylylation is needed.

55 ular dynamics (MD) simulations indicate that adenylylation is required for acetylation-dependent acti

56 suppressed overall nick ligation and ligase adenylylation, it did not compromise sealing at a preade

57 tacts at the nick and with ATP during ligase adenylylation; (iv) the role of Phe-44 in forming the pr

58 s (k(step3) = 25 s(-1)) exceeds that for DNA adenylylation (k(step2) = 2.4 s(-1)) and that Mg(2+) bin

59 erase and ATPase activities, which reveal an adenylylation mechanism involved in a two-step catalytic

60 enterica growth caused by the expression of adenylylation-mimetic GS is rescued by acetylation-mimic

61 The IbpA-mediated adenylylation occurs on a functionally critical tyrosine

62 report that Escherichia coli RtcA catalyzes adenylylation of 5'-phosphate ends of DNA or RNA strands

63 Subsequent adenylylation of enzyme prevents rebinding to the adenyl

64 It was shown that adenylylation of FMN is reversible; FAD and pyrophosphat

65 re potent than GlnK in the activation of the adenylylation of glutamine synthetase by ATase.

66 phosphatase activity of NRII, and stimulated adenylylation of GS by ATase.

67 The adenylylation of GS plays no significant role in nif exp

68 autoinhibition and thus allowing subsequent adenylylation of its target, the DNA gyrase subunit GyrB

69 enylylated ( approximately 30%), whereas the adenylylation of M. bovis BCG GS does not change.

70 The site of adenylylation of MTb GS by the E. coli ATase is Tyr-406,

71 g these is DrrA/SidM, which catalyzes stable adenylylation of Rab1b, a regulator of endoplasmatic ret

72 sence of [alpha-(32)P]ATP, which resulted in adenylylation of Rho GTPases and cytoskeletal disruption

73 Nick sensing also requires adenylylation of Rnl2.

74 In some bacteria, GS is regulated via adenylylation of some or all of the subunits within the

75 Equilibrium constants for the adenylylation of T4 DNA ligase have been measured at 10

76 rates the second step of the ligase pathway (adenylylation of the 5'-PO4 strand) by a factor of 1000,

77 er, but dispensable for nick recognition and adenylylation of the 5'-PO4 strand.

78 protein, and the reversible modification as adenylylation of the conserved tyro-sine 51 residue that

79 ride linkage in ATP significantly facilitate adenylylation of the enzyme.

80 D) cofactor biosynthesis, which catalyze the adenylylation of the nicotinic acid mononucleotide (NaMN

81 mechanism based on the loop motions in which adenylylation of the Tyr397 loop reverses the effect of

82 Tase is Tyr-406, as indicated by the lack of adenylylation of the Y406F mutant, and, as expected, is

83 of Tyr-326 together with either nitration or adenylylation of Tyr-397 leads to inactivation of the en

84 It was further established that adenylylation of Tyr-397 precludes its nitration by pero

85 We have established the effects of adenylylation on Rab1 interactions and properties in a q

86 by cyclic AMP (FIC)-domain enzymes catalyze adenylylation or other posttranslational modifications o

87 rovide important structural insight into the adenylylation reaction mechanism catalyzed by Fic domain

88 amide mononucleotide (NMN) occurs before the adenylylation reaction, which converts this alternative

89 tion induced by cAMP) domain, catalyzes this adenylylation reaction.

90 stinctive catalytic mechanisms of the lysine adenylylation reaction.

91 sting that the 3' de-guanylylation and 5' de-adenylylation reactions follow the same pathway of nucle

92 bolished by mutation of the predicted lysine adenylylation site (Lys-165) in the C-terminal domain an

93 lutarate concentration, the regulation of GS adenylylation state by glutamine was sharper and occurre

94 We examined the regulation of GS adenylylation state in a reconstituted system containing

95 se (ATase, the glnE product) to regulate the adenylylation state of glutamine synthetase (GS).

96 s reconstituted bicyclic cascade system, the adenylylation state of GS was regulated reciprocally by

97 We also compared the regulation of GS adenylylation state to the regulation of phosphorylation

98 a substrate for E. coli ATase, but only low adenylylation states are accessible.

99 This parallels the low adenylylation states observed for GS from mycobacteria a

100 Whereas the RtcA adenylylation step is metal-catalyzed, the subsequent st

101 7 carboxylate, exclusively during the ligase adenylylation step.

102 fs I, IV, and V and suffices for both enzyme adenylylation (step 1 of the ligation pathway) and phosp

103 tional effects on the isolated steps of Rnl1 adenylylation (step 1) and phosphodiester bond formation

104 3'OH/5'PO(4) nicks in duplex RNAs via ligase adenylylation (step 1), AMP transfer to the nick 5'PO(4)

105 eps of the ligation pathway including ligase-adenylylation (step 1), RNA adenylylation (step 2) and p

106 including ligase-adenylylation (step 1), RNA adenylylation (step 2) and phosphodiester bond synthesis

107 3'-OH nucleoside in the catalysis of DNA 5'-adenylylation (step 2) and phosphodiester synthesis (ste

108 dysfunctional by virtue of defects in ligase adenylylation: T163A, H167A, G168A, K186A, E230A, F281A

109 Enzyme self-adenylylation was confirmed to also occur on a fast time

110 A two-metal mechanism of lysine adenylylation was established via a crystal structure of

111 Abortive adenylylation was suppressed at low ATP concentrations (