ライフサイエンスコーパス: agarose

1 made out of a hybrid hydrogel (8% gelatin/1% agarose).

2 0.5 mug (for polyacrylamide) to 2.5 mug (for agarose).

3 specimen from a pin or embedding it in 1-2% agarose.

4 o Dictyostelium cells chemotaxing under soft agarose.

5 and EC-HA) or covalent attachment to glyoxal agarose.

6 he aldehyde-modified protein using hydrazide-agarose.

7 ty-purified using human beta(2)-GP1 bound to agarose.

8 onventional aqueous gels such as gelatin and agarose.

9 onto an activated support of glutaraldehyde agarose.

10 be mechanically robust when inserted into 2% agarose.

11 ties to hydrolyze the glycosidic linkages in agarose, a linear polymer comprising the repeating disac

12 ional sites on ion-exchange ligands based on agarose, a support matrix routinely used in protein chro

13 was expressed in E. coli, purified by Ni-NTA agarose affinity chromatography and functionally charact

14 pressed in E. coli, and purified with Ni-NTA agarose affinity chromatography.

15 try and CBD-mimetic peptides, as well as CaM-agarose affinity pulldown of full-length recombinant BdA

16 g viable and functional pancreatic islets in agarose-agarose macrobeads (diameter 6-8 mm) might provi

17 orporated into two different hydrogel films, agarose and a copolymer of acrylamide and 2-(dimethylami

18 ell culture by Pd nanosheets captured within agarose and alginate hydrogels, providing a biodegradabl

19 ucleic acid probes embedded within permeable agarose and alginate hydrogels.

20 carbohydrate but not related substrates like agarose and carrageenan.

21 inase pathway results in slow movement under agarose and cells that produce few blebs, though actin p

22 hly sensitive toward carbohydrates-on native agarose and chemically modified agarose in the gel phase

23 The film of blended agarose and chitosan allows good dispersion of C18, prev

24 KHDC1A and 1B bind polyU agarose and form oligomers like other KH-domain proteins

25 Agarose and gelatin form non-interactive bicontinuous ph

26 using nickel-nitrilotriacetic acid (Ni-NTA) agarose and incubate the immobilized TFs with fluorescen

27 Hybrid films of agarose and lipids even enabled the formation of giant l

28 Hydrating the films of agarose and lipids in aqueous salt solutions resulted in

29 tion of giant liposomes from hybrid films of agarose and lipids in comparison to established methods

30 ows that gentle hydration of hybrid films of agarose and lipids is a simple, rapid, and reproducible

31 UVs spontaneously swell from hybrid films of agarose and lipids.

32 oceeded from hybrid films of partially dried agarose and lipids.

33 hyranases) have been characterized on simple agarose and more rarely on porphyran, a polymer containi

34 n optimized to migrate appropriately on both agarose and native polyacrylamide, unlike many currently

35 d degradation fragments were quantified with agarose and polyacrylamide gel electrophoresis and immun

36 Agarose and polyacrylamide gel electrophoresis systems f

37 Most of the GIP were retained by the G12-agarose and represented the largest part of the immunoge

38 nity chromatography with scaffolding protein-agarose and scaffolding protein shell re-entry experimen

39 ons have on the selective adsorption between agarose and SWCNTs suspended with sodium dodecyl sulfate

40 Amylopectin, agarose, and aspartic acid exhibited similar critical ic

41 otin-N-maleimide, purified with streptavidin-agarose, and resolved by 2-dimensional gel electrophores

42 ized biocatalyst was developed using glyoxyl-agarose as support; it exhibited high performance in deg

43 This review aims at a classification of agarose-based biomaterials and their derivatives applica

44 The ever-growing use of agarose-based biomaterials for drug delivery systems res

45 ciated with the future developments ahead of agarose-based biomaterials in the realm of advanced drug

46 dded fluorescent microplastics in artificial agarose-based food and offered the food to marine isopod

47 le screen printed electrode modified with an agarose-based hydrogel deposit to monitor bacterial grow

48 For agarose-based systems, the suitability of different agar

49 with prevascularization of the graft bed by agarose-basic fibroblast growth factor.

50 tachment of DNA sequencing libraries onto an agarose bead support enables repetitive primer extension

51 gest that, due to the porous interior of the agarose bead, internal analyte transport is both diffusi

52 rcle amplification (RCA) bioassay and an (2) agarose bead-based microfluidic device for the affinity

53 hich relies on the encapsulation of cells in agarose beads and labeling breaks directly and specifica

54 Thus, this work used BSA immobilized in agarose beads as a novel solid-phase extraction method f

55 CypD(-/-) mice to immunoprecipitation using agarose beads coupled to antiacetyl lysine antibodies fo

56 Monocytes migrating toward agarose beads impregnated with conditioned media from M.

57 capture using NeutrAvidin or concanavalin A agarose beads or directly via covalent coupling of free

58 000 CFU Pseudomonas aeruginosa embedded into agarose beads to slow clearance.

59 led to nickel nitrilotriacetic acid (Ni-NTA) agarose beads, specifically recruited F-actin in the pre

60 was determined by pull-down assays with PAK-agarose beads.

61 mouse GPIHBP1 and used that antibody to coat agarose beads.

62 teraction using metabolite immobilization on agarose beads.

63 N-hydroxysuccinimide (NHS) ester attached on agarose beads.

64 n and affinity chromatography on pepstatin-A agarose bed the aspartyl proteases were purified and con

65 as immobilized on sodium alginate as well as agarose bed.

66 mutant PTEN to bind ATP was assessed by ATP-agarose-binding assays.

67 e procedure of introducing gaps by digesting agarose blocks imbedded in the cell sheets.

68 demonstrate that ZgAgaC hydrolyzes not only agarose but also complex agars from Ceramiales species.

69 talyzes a critical step in the metabolism of agarose by S. degradans through cleaving agarose oligome

70 Heme agarose captured ZnuD in enriched outer membrane fractio

71 ing muscle movements by embedding embryos in agarose caused similar defects in peripheral RB axon gui

72 Agarose-chitosan-immobilized octadecylsilyl-silica (C18)

73 ed from murine serum by gelatin cross-linked agarose chromatography and subsequently was enzymaticall

74 he flow-through fraction upon subsequent ATP-agarose chromatography.

75 Cells were grown in agarose-coated 96 well plates, forming reproducible 1-mm

76 d the deposition of complement components on agarose-coated plates, although it could not remove prev

77 separated on a nickel-nitrilotriacetic acid-agarose column based on the number of His tags present i

78 Escherichia coli BL21 and purified by Ni-NTA agarose column.

79 Constructed BSA-agarose columns could extract OTA efficiently from red w

80 on agarose was also found equal to 120 s at agarose concentration of 1.2% (w/v) and optical density

81 volumes were used, but was not influenced by agarose concentration or the presence of ethidium bromid

82 the range of 1:170 to 1:3100 for beads with agarose concentration ranging from 0.5% to 8% for the se

83 the suitability of different agarose types, agarose concentrations, and buffer systems was determine

84 the passive diffusion response of dextran in agarose confirms the applicability of Fick's law of diff

85 e developed and tested a method using hollow agarose cylinders designed to accommodate for embryonic

86 The mechanical properties of agarose-derived hydrogels depend on the scaffolding of t

87 Diffusion coefficients (D) through agarose diffusive gels ranged from (1.02 to 4.74) x 10(-

88 to loading using a model system of acellular agarose disks and dextran in phosphate-buffered saline (

89 ntly enhanced the rate of solute uptake into agarose disks, relative to static loading.

90 Edges that are bordered by agarose do not induce activation of the EGFR, indicating

91 o-processive mode of action of Aga50D on the agarose double helix.

92 onsequently, the permanent dipole moments of agarose drastically reduces the retention of SWCNTs.

93 d due to substantial release of encapsulated agarose during temperature treatment.

94 We report that crosslinked agarose (e.g., Sepharose) chromatography medium that has

95 we developed a single-cell protocol based on agarose-embedded bisulfite treatment, which allows inves

96 -dependent intracellular Ca(2+) signaling in agarose-embedded chondrocytes, and then used this model

97 rometer-resolution 3D images of paraffin- or agarose-embedded whole organs with high fidelity, achiev

98 including how to hold mouse embryos without agarose embedding, how to transfer embryos without air e

99 ble layers (EDL) surrounding the NPs and the agarose fibres.

100 Of the materials studied, an anthocyanin-agarose film is nominated as the optimum materials with

101 Further fractionation of the ATP-agarose flow-through on Sephacryl S-300 separates free t

102 are heated above the melting temperature of agarose for 2 h before use, vesicle response is (partial

103 h as ribosomal RNAs and their precursors, on agarose-formaldehyde gels.

104 lowed down for agarose-GUVs when compared to agarose-free GUVs.

105 te were successfully inserted 2 cm-deep into agarose gel "brain phantoms" and into rat brains under c

106 balloon (cerebrospinal fluid and blood) and agarose gel (brain).

107 replication models based on two-dimensional agarose gel analyses.

108 was assessed by Western blotting and native agarose gel analysis in Huh7 cells, and the human immune

109 have been experimentally validated by QPCR, agarose gel analysis, sequencing and BLAST, and all vali

110 205 squamous carcinoma cells embedded in an agarose gel and cell spheroids in Matrigel.

111 determined from 32 phantoms constructed with agarose gel and in eight concentrations from each of the

112 The same irradiated samples were analyzed by agarose gel and SSB yields were determined by convention

113 tilting angle of the microtubules buried in agarose gel and to find the precise surface plasmon reso

114 10-, 20-, and 30-mmol/L NaCl solution and 5% agarose gel as a reference.

115 MVm and C5a) compared with WT using an under-agarose gel chemotaxis assay.

116 In this protocol, droplets of agarose gel containing different chemokines are applied

117 s ever observed for compression of uncharged agarose gel controls.

118 present work proposes the improvement of an agarose gel DNA electrophoresis in order to allow for a

119 describe the development of 2D intact mtDNA agarose gel electrophoresis (2D-IMAGE) for the separatio

120 eosomal arrays were determined by analytical agarose gel electrophoresis (AAGE) and single molecules

121 d in vitro using a low-temperature EDTA-free agarose gel electrophoresis (LTEAGE) procedure.

122 The quantity and quality were confirmed by agarose gel electrophoresis and polymerase chain reactio

123 y a dye-doped silica shell were separated by agarose gel electrophoresis and scanned by a conventiona

124 urse of infection by one and two-dimensional agarose gel electrophoresis and Southern hybridization.

125 Agarose gel electrophoresis and ultraviolet spectrophoto

126 reaction (PCR) are exploited using on-column agarose gel electrophoresis as separation and inductivel

127 By adopting a native agarose gel electrophoresis assay that can specifically

128 capsids that migrated more slowly in native agarose gel electrophoresis from A36V mutant than from t

129 In the current work, an improved agarose gel electrophoresis method for analysis of high

130 The widely used agarose gel electrophoresis method for assessing radiati

131 Agarose gel electrophoresis of DNA and RNA is routinely

132 minimize errors and is broadly applicable to agarose gel electrophoresis of RNA samples and their sub

133 Marker validation by PCR and low-cost agarose gel electrophoresis revealed that 92.5% were pol

134 ild-type and mutant plants by single-nucleus agarose gel electrophoresis revealed that bleomycin-indu

135 Agarose gel electrophoresis showed that high molecular w

136 We show by two-dimensional (2D) agarose gel electrophoresis that replication forks natur

137 genetics and high resolution two-dimensional agarose gel electrophoresis to examine the torsional ten

138 We apply agarose gel electrophoresis to sensitively evaluate prot

139 Agarose gel electrophoresis was subsequently used to sep

140 SSRs are robust (with basic PCR methods and agarose gel electrophoresis), informative, and applicabl

141 PCR amplification, agarose gel electrophoresis, and sequencing methods were

142 Agarose gel electrophoresis, circular dichroism and diff

143 are based on two-dimensional, non-denaturing agarose gel electrophoresis, followed by structure deter

144 Using two-dimensional agarose gel electrophoresis, we examined DNA replication

145 Using one- and two-dimensional agarose gel electrophoresis, we show that the linear mtD

146 gher sensitivity as compared to conventional agarose gel electrophoresis.

147 ng transmission electron microscopy and 1.2% agarose gel electrophoresis.

148 ugation, and retarded mobility during native agarose gel electrophoresis.

149 when positive, and results were verified by agarose gel electrophoresis.

150 ed, as revealed by semi-denaturing detergent agarose gel electrophoresis.

151 d transfer complex can be isolated by native agarose gel electrophoresis.

152 gut cells and DNA fragmentation analyses by agarose gel electrophoresis.

153 is beyond the resolution capacity of regular agarose gel electrophoresis.

154 e last annealing cycle are separated by 4-6% agarose gel electrophoresis.

155 wed by amplifications with ISSR and SCoT and agarose gel electrophoresis.

156 by bovine pancreatic trypsin immobilised on agarose gel in 100 mM ammonium hydrocarbonate buffer, pH

157 ylococcus aureus strains were deposited onto agarose gel modified electrodes which contained clinical

158 %) and without contaminants such as residual agarose gel or DNA intercalating dyes.

159 mical laser-induced nucleation of an aqueous agarose gel prepared with supersaturated potassium chlor

160 ted by the electrophoresis in polyacrylamide/agarose gel profile.

161 s measured, and the results suggest that the agarose gel reduces the effective supersaturation of the

162 ent pH changes throughout a nanowire network/agarose gel sample during external solution pH changes,

163 The agarose gel stabilizes the boundary between the leading

164 were separated by electrophoresis on a 0.8% agarose gel stained with ethidium bromide.

165 lts demonstrate that any modification to the agarose gel surface and, consequently, the permanent dip

166 iffuse up to 2 orders of magnitude faster in agarose gel than their hard NP counterparts.

167 cted a new nucleoprotein complex on a native agarose gel that was produced in the presence of >200 nM

168 that saccharides systematically decrease the agarose gel thinning rate up to a factor two, and exempl

169 domly distributed fluorescent nanospheres in agarose gel were obtained and fitted with the theoretica

170 e pH change, measured using tissue-mimicking agarose gel, extends to 0.8 cm(3) in volume within an ho

171 al diameter, 0.579 mm) shallowly embedded in agarose gel, then to a collection reservoir.

172 solid materials including asphalt concrete, agarose gel, vaginal tissue, polymer, agar, bone, spider

173 Seven melt-curve and agarose gel-based mismatch amplification mutation assays

174 s as easy, convenient, and inexpensive as an agarose gel.

175 digestion, and detection of fragments on an agarose gel.

176 ass substrate while they are diffusing in an agarose gel.

177 d Hb with the ratio 1:1 was characterized by agarose gel.

178 ere analyzed by electrophoresis performed on agarose gel; samples with a discrete or localized band w

179 purifying DNA-origami nanostructures rely on agarose-gel electrophoresis (AGE) for separation.

180 We have exploited the separation power of agarose-gel electrophoresis to purify milligram amounts

181 were isolated from the gel and reanalyzed by agarose-gel electrophoresis, single-nanoparticle-upconve

182 e-specific PCR qualitative assay followed by agarose-gel electrophoresis.

183 ing the uptake of (45)Ca by isolated ACVs in agarose gels and by ACVs in situ in freeze-thawed cartil

184 migrated with undigested parental capsids on agarose gels and cosedimented with undigested capsids by

185 Both increased polymer concentration in agarose gels and increased cross-linking density in algi

186 Agarose gels are viscoelastic soft solids that display a

187 Using carboxylated agarose gels as a screening platform, we demonstrate tha

188 it is found that data from both alginate and agarose gels collapse onto the same curve.

189 trap the synaptic complex observed on native agarose gels correlated with its potency for inhibiting

190 Selective adsorption onto agarose gels has become a powerful method to separate si

191 ing kinetics and compare the drying speed of agarose gels loaded with various non-gelling saccharides

192 ings imply that the SSB yields inferred from agarose gels need reevaluation, especially when they wer

193 tudy by in-situ interferometry the drying of agarose gels of various compositions cast in Petri dishe

194 sensors in conjunction with collagen-coupled agarose gels to detect subcellular activities of SFK and

195 ted muscle fibers within biochemically inert agarose gels tuned to mimic native tissue stiffness.

196 ection of 10 muL cell inclusions in cm-sized agarose gels used here as phantom models of microtumors.

197 To probe that question, U(IV) immobilized in agarose gels was exposed to conditions allowing biologic

198 Heterogeneity within agarose gels was modeled by assuming that fiber-rich, sp

199 We use micropatterned, enzyme-laden agarose gels which are stamped on polyacrylamide films c

200 els, reconstituted basement membrane matrix, agarose gels, alginate gels, and fibrin gels, but not in

201 or 12 h, separated by electrophoresis on 2 % agarose gels, and visualized with ethidium bromide stain

202 ined without the need of fluorescent probes, agarose gels, melting curves or sequencing analysis.

203 de fluorescence of PCR products excised from agarose gels.

204 ctrophoretic transport of lambda-DNA through agarose gels.

205 DNA that can be visualized in vivo using 2D agarose gels.

206 etected as the corresponding PCR amplicon in agarose gels.

207 ecular mass as low as approximately 9 kDa in agarose gels.

208 breast cancer cells seeded into nonadhesive agarose gels.

209 e this behavior by embedding microgel NPs in agarose gels.

210 teroduplex DNA hybrids in high concentration agarose gels.

211 Titin isoform expression was evaluated with agarose gels.

212 orescently labeled flagellar filaments at an agarose-glass interface.

213 n be purified using Galanthis nivalis lectin agarose (GNA), but this technique is suboptimal for glob

214 When the agarose-GUVs are heated above the melting temperature of

215 ngs reveal potential artifactual behavior of agarose-GUVs in processes involving morphological change

216 racterize the mechanical properties of these agarose-GUVs in response to electric pulses, which induc

217 f poration, is significantly slowed down for agarose-GUVs when compared to agarose-free GUVs.

218 As a result, agarose has received particular attention in the fabrica

219 rylic acid (PAA), polyacrylamide (PAAm), and agarose hydrogel spheres on smooth surfaces.

220 nsor Lumisens III using immobilized cells in agarose hydrogel, allowed to detect artificial mercury c

221 is formed between an aqueous droplet and an agarose hydrogel, which allows imaging in addition to el

222 mechanical properties of chondrocyte-seeded agarose hydrogels relative to unloaded free swelling con

223 on of the vesicles were found to encapsulate agarose in the form of a gel-like meshwork.

224 es-on native agarose and chemically modified agarose in the gel phase for the first time.

225 rmed using 7.5% (w/w) gelatin and 1.5% (w/w) agarose in the presence of variable amounts of polydextr

226 In our strategy, monodisperse 1.5 nL agarose-in-oil droplets are produced with a high frequen

227 Agarose-in-plug and capillary assays showed that these t

228 oes not require the sample to be embedded in agarose; instead, samples are prepared conventionally on

229 Agarose is a prominent marine polysaccharide representin

230 However, agarose is left encapsulated in the vesicles in differen

231 physically constrained by an inert material (agarose), is sufficient to induce formation of purse str

232 s of hydrogels derived from polysaccharides (agarose, kappa-carrageenan) having an alpha-helical back

233 M gene reduced bacterial replication on 0.3% agarose low Mg(2+) media but not in low Mg(2+) liquid me

234 acillus thermocatenulatus lipase 2, BTL2) on agarose macroporous beads, followed by covalent coupling

235 template copies/reaction, while that of the agarose-MAMAs ranged from 10(3) to 10(5) template copies

236 CC formed more spheroids (orospheres) in 3-D agarose matrices or ultra-low attachment plates than con

237 The nuclei are then embedded in an agarose matrix containing numerous pores, allowing the a

238 Immobilization of a potent GSM onto an agarose matrix quantitatively recovered Pen-2 and to a l

239 a growth-restricting hydrogel composed of an agarose matrix with a second coating of agarose to form

240 aryotic cells, but because it is based on an agarose matrix, it is not always optimal for all protein

241 purified and subsequently manipulated in the agarose matrix.

242 now report that Salmonella can move on 0.3% agarose media in a flagella-independent manner when expe

243 ere found to readily germinate even on water agarose medium.

244 on mass spectrometric imaging (MALDI-MSI) of agarose micro-beads randomly arrayed at high-density in

245 Here, 3D-concave agarose micro-wells were used to culture robust pancreat

246 , we chose an anchoring molecule composed of agarose microbeads functionalized with an Fc-binding dom

247 Porous agarose microbeads, with high surface to volume ratios a

248 rmed using alpha-chymotrypsin immobilised on agarose microparticles.

249 uents of sweat) are collected into hydrogel (agarose) micropatches.

250 dissociated zebrafish retinal progenitors in agarose microwells.

251 HA mobility in 0.5% agarose minigels was found to be linearly related to the

252 ls how random, three-dimensional networks of agarose nanofibers are incorporated into single crystals

253 ydextrose prevents the formation of a stable agarose network, with the polysaccharide chains dispersi

254 carbon nanotubes (SWNTs) confined in porous agarose networks.

255 of agarose by S. degradans through cleaving agarose oligomers into neoagarobiose products that can b

256 is encased either in a rectangular block of agarose or between Formvar films suspended on a wire loo

257 nhibiting cell-free virus transmission using agarose or neutralizing antibodies, we show that EGCG in

258 munoprecipitated with the HC-HA complex from agarose-overlaid AM cell extracts by an anti-human Ialph

259 Using an agarose overlay to trap the HA-containing matrix, the HC

260 roblasts form the HC-HA-PTX3 complex with an agarose overlay.

261 tly pseudopods to blebs when migrating under agarose overlays of increasing stiffness.

262 IMER) is compared to that of a conventional, agarose packed bed, pepsin IMER column commonly used in

263 volves seeding and growing bacteria on small agarose pads and imaging the resulting microcolonies.

264 In this study, agarose particles were modified with MANAE, PEI and glyo

265 obilized on the surface of a 50 muM magnetic agarose particles, the limit of detection of rcHRPII was

266 he proposed technique was validated by using agarose phantoms.

267 rabidopsis thaliana) seedling roots grown on agarose plates.

268 In the presence of poration, agarose polymers prevent complete pore closure and lead

269 t inhibited when plasmids are immobilized in agarose prior to addition of egg extract.

270 tform, we demonstrate that soft-carboxylated agarose provides a unique environment for the polarizati

271 SA-agarose pulldown and immunoblotting for IRF5 were used t

272 vacuolar hemin exporter, results with hemin-agarose pulldown assays showed that Abc3 binds to hemin.

273 nalysis by absorbance spectroscopy and hemin-agarose pulldown assays showed that Shu1 interacts with

274 T1A into giant unilamellar vesicles using an agarose rehydration method.

275 of S. marcescens secretomes with polymyxin B agarose rendered secretomes unable to inhibit epithelial

276 n their P3 coat protein were bound to nickel agarose resin and were subsequently challenged with a pr

277 d to standard, commercially available Ni-NTA agarose resin.

278 ancer cells (OEC-M1) were encapsulated in 3D agarose scaffold and cultured in a miniaturized chamber

279 Agarose sensors showed a 40 nm wavelength shift from 0 t

280 Comparison of cells in 1% and 3% agarose showed that cells in the stiffer gels rapidly de

281 Affinity assays using cGMP-immobilized agarose showed that only activated PKGIalpha binds RhoA,

282 The melted agarose solution containing a redox dye tetranitroblue t

283 urthermore, we show that migration under the agarose spot can be modulated by selective small molecul

284 against this gradient by crawling under the agarose spots towards their centre.

285 okine gradients by a simple stamping method: agarose stamps were soaked with chemokine solution to fo

286 s in monolayer culture and three-dimensional agarose, suggesting a role for cell adhesion.

287 e integrative repair by GFP+ cells seeded in agarose, supporting their potential use in cartilage the

288 Derivatized-agarose supports are suitable for enzyme immobilization

289 f an agarose matrix with a second coating of agarose to form 6- to 8-mm diameter macrobeads.

290 -based systems, the suitability of different agarose types, agarose concentrations, and buffer system

291 rase AgaD requires at least four consecutive agarose units (DP8) and is highly intolerant to modifica

292 ably softer hydrogels made from carboxylated agarose use a scaffold of unpaired beta-strands.

293 The bacteria that metabolize agarose use multiple enzymes of complementary specificit

294 trates, we determined that ZgAgaC recognizes agarose via a mechanism different from that of classical

295 We show that CbpA binds to cAMP-conjugated agarose via its C-terminal CAP domain.

296 response time of immobilized T. thioparus on agarose was also found equal to 120 s at agarose concent

297 ctyostelium cells were observed moving under agarose, which efficiently induces blebbing, and the dyn

298 helix in the highly rigid hydrogel of native agarose, while the considerably softer hydrogels made fr

299 circuit boards, made with centimeter-length agarose wires.

300 We encapsulated bacteria in agarose with a user-defined stiffness, measured the grow