ライフサイエンスコーパス: agarose gel

1 rcoiled (sc) form of plasmid DNA (pDNA) from agarose gel.

2 ering was evaluated by electrophoresis on 3% agarose gel.

3 lso used to extract the sc form of pDNA from agarose gel.

4 as quantitated by radial diffusion in fibrin-agarose gel.

5 .22 in solution to 1.2 +/- 0.04 in a 3 w/v % agarose gel.

6 bilayer is sandwiched between two layers of agarose gel.

7 native conditions through polyacrylamide or agarose gel.

8 slationally immobile in a low weight percent agarose gel.

9 double-stranded DNA that was detected on an agarose gel.

10 d Hb with the ratio 1:1 was characterized by agarose gel.

11 resembles a simple salt solution as in a 4% agarose gel.

12 ia diffusion into the network that forms the agarose gel.

13 Expulsion occurs in an agarose gel.

14 e and subjected to electrophoresis on a 1.5% agarose gel.

15 the density of MIMIC to target cDNA bands on agarose gel.

16 lectrophoresis of digestion products in 1.5% agarose gel.

17 idyl-prolyl isomerase-were immobilized on an agarose gel.

18 s of iron(III) immobilized on iminodiacetate-agarose gel.

19 ere identified after electrophoresis in 1.5% agarose gel.

20 s as easy, convenient, and inexpensive as an agarose gel.

21 digestion, and detection of fragments on an agarose gel.

22 ass substrate while they are diffusing in an agarose gel.

23 ion in the Darcy permeability of 3 vol/vol % agarose gels.

24 teroduplex DNA hybrids in high concentration agarose gels.

25 restriction of translational mobility in 1% agarose gels.

26 to resemble spherical obstacles embedded in agarose gels.

27 esis in 20% polyacrylamide-8 M urea gels and agarose gels.

28 PCR products were detected in agarose gels.

29 per band in post-electrophoretically stained agarose gels.

30 formation of supershifted species on native agarose gels.

31 nds, having slightly different mobilities in agarose gels.

32 und to be applicable with 0.8, 1.0, and 2.0% agarose gels.

33 ansformants had an aberrant mobility through agarose gels.

34 ysis of restriction digests on nondenaturing agarose gels.

35 Titin isoform expression was evaluated with agarose gels.

36 de fluorescence of PCR products excised from agarose gels.

37 ctrophoretic transport of lambda-DNA through agarose gels.

38 DNA that can be visualized in vivo using 2D agarose gels.

39 e this behavior by embedding microgel NPs in agarose gels.

40 etected as the corresponding PCR amplicon in agarose gels.

41 ecular mass as low as approximately 9 kDa in agarose gels.

42 breast cancer cells seeded into nonadhesive agarose gels.

43 rystals can be achieved by crystal growth in agarose gel, a naturally occurring chiral polysaccharide

44 els, reconstituted basement membrane matrix, agarose gels, alginate gels, and fibrin gels, but not in

45 replication models based on two-dimensional agarose gel analyses.

46 The conditions used for 2D agarose gel analysis are favorable for branch migration

47 was assessed by Western blotting and native agarose gel analysis in Huh7 cells, and the human immune

48 have been experimentally validated by QPCR, agarose gel analysis, sequencing and BLAST, and all vali

49 fined plasmid substrates and two-dimensional agarose gel analysis, we examined the collision of an ac

50 rk integrity as monitored by two-dimensional agarose gel analysis.

51 205 squamous carcinoma cells embedded in an agarose gel and cell spheroids in Matrigel.

52 determined from 32 phantoms constructed with agarose gel and in eight concentrations from each of the

53 maging studies, in infusion experiments with agarose gel and in vivo rat brain studies simulating cli

54 Ligation was verified by agarose gel and small-angle X-ray scattering.

55 The same irradiated samples were analyzed by agarose gel and SSB yields were determined by convention

56 es derived from densitometric scanning of an agarose gel and those derived from the SCFluo method wer

57 tilting angle of the microtubules buried in agarose gel and to find the precise surface plasmon reso

58 adily assayed by electrophoresis on standard agarose gels and because a public database of over 25,00

59 ing the uptake of (45)Ca by isolated ACVs in agarose gels and by ACVs in situ in freeze-thawed cartil

60 ells were identified by DNA fragmentation in agarose gels and by Hoechst staining.

61 migrated with undigested parental capsids on agarose gels and cosedimented with undigested capsids by

62 results in enhanced 3 dimensional growth in agarose gels and in long-term cultures within matrigel.

63 Both increased polymer concentration in agarose gels and increased cross-linking density in algi

64 nated the need to load, run, stain, and read agarose gels and provided the advantage of instant detec

65 fI, followed by submarine electrophoresis in agarose gels and staining with ethidium bromide, produce

66 ladder-like fragmentation of genomic DNA in agarose gels and the intense blue fluorescence exhibited

67 on to all AP-PCR profiles was extracted from agarose gels and then cloned and sequenced.

68 13 were positive only by the assay with the agarose gel, and 3 were positive only by the assay with

69 g protein calmodulin (CaM) immobilized in an agarose gel, and we have demonstrated the application of

70 d (female) were isolated, electrophoresed in agarose gels, and transferred to nylon membranes.

71 or 12 h, separated by electrophoresis on 2 % agarose gels, and visualized with ethidium bromide stain

72 Gels are run horizontally in a standard mini-agarose gel apparatus.

73 Agarose gels are viscoelastic soft solids that display a

74 10-, 20-, and 30-mmol/L NaCl solution and 5% agarose gel as a reference.

75 Using carboxylated agarose gels as a screening platform, we demonstrate tha

76 ligonucleosomal fragmentation was visible on agarose gels as early as 60 or 30 min after PDT, respect

77 Native agarose gel assays corroborated this finding.

78 in protocol A by increasing the sigma on the agarose gel at a constant rate to define the cardiocyte

79 ision and integration, we first developed an agarose gel-based assay for CTnDOT recombination, which

80 PCR with urine combined with agarose gel-based detection was 66.9% sensitive and 98.3

81 ene, designated UROC28, was identified by an agarose gel-based differential display technique, and it

82 Seven melt-curve and agarose gel-based mismatch amplification mutation assays

83 sis-based five-enzyme (SNaPshot) method, the agarose gel-based one-enzyme method, and the automatic s

84 te were successfully inserted 2 cm-deep into agarose gel "brain phantoms" and into rat brains under c

85 balloon (cerebrospinal fluid and blood) and agarose gel (brain).

86 n be directly visualized after separation in agarose gels by ethidium bromide staining.

87 monella specific on ethidium bromide-stained agarose gels by Southern hybridization with a 20-mer oli

88 The pore size, a, of a 2% agarose gel cast in a 0.1 M PBS solution was estimated.

89 Using two-dimensional agarose gels, chemical probing and atomic force microsco

90 MVm and C5a) compared with WT using an under-agarose gel chemotaxis assay.

91 it is found that data from both alginate and agarose gels collapse onto the same curve.

92 nded DNA fragments with various DNA markers, agarose gel concentrations, and field strengths.

93 DNA agarose gels confirmed morphological identification of a

94 In this protocol, droplets of agarose gel containing different chemokines are applied

95 s ever observed for compression of uncharged agarose gel controls.

96 trap the synaptic complex observed on native agarose gels correlated with its potency for inhibiting

97 present work proposes the improvement of an agarose gel DNA electrophoresis in order to allow for a

98 the presence of nucleosomal DNA fragments on agarose gels (DNA ladder) and in situ nick end labeling.

99 describe the development of 2D intact mtDNA agarose gel electrophoresis (2D-IMAGE) for the separatio

100 eosomal arrays were determined by analytical agarose gel electrophoresis (AAGE) and single molecules

101 HPLC analysis was validated in parallel with agarose gel electrophoresis (AGE), enzyme digestion, and

102 rformance liquid chromatography (AEC) and an agarose gel electrophoresis (AGE)-based method developed

103 d in vitro using a low-temperature EDTA-free agarose gel electrophoresis (LTEAGE) procedure.

104 luated PCR product detection by using either agarose gel electrophoresis (PCR-gel) or dot blot hybrid

105 d tissue was measured using a combination of agarose gel electrophoresis and a radiometric assay.

106 block architectures were characterized by 1% agarose gel electrophoresis and atomic force microscope

107 d tubules and 20 glomeruli were separated by agarose gel electrophoresis and by isoelectric focusing,

108 Apoptosis was determined using agarose gel electrophoresis and by measuring the cytopla

109 We used two-dimensional agarose gel electrophoresis and electron microscopy to a

110 With alkaline agarose gel electrophoresis and filter blot hybridizatio

111 NE increased DNA laddering on agarose gel electrophoresis and increased the percentage

112 ling, aggregation, loss of resolution during agarose gel electrophoresis and loss of transformation a

113 n comparing MIRU-VNTR profiles obtained from agarose gel electrophoresis and PCRs analyzed on a WAVE

114 The quantity and quality were confirmed by agarose gel electrophoresis and polymerase chain reactio

115 DNA fragmentation determined by agarose gel electrophoresis and quantitation of [3H]thym

116 y a dye-doped silica shell were separated by agarose gel electrophoresis and scanned by a conventiona

117 urse of infection by one and two-dimensional agarose gel electrophoresis and Southern hybridization.

118 t method, the DNA segments were separated by agarose gel electrophoresis and stained with ethidium br

119 ucts (e.g., open circle and linear forms) by agarose gel electrophoresis and subsequently quantified

120 csL was also examined at pH 8.0 by using SDS-agarose gel electrophoresis and transmission electron mi

121 Agarose gel electrophoresis and ultraviolet spectrophoto

122 reaction (PCR) are exploited using on-column agarose gel electrophoresis as separation and inductivel

123 By adopting a native agarose gel electrophoresis assay that can specifically

124 Native agarose gel electrophoresis confirmed that FliH and FliI

125 ation of density gradient centrifugation and agarose gel electrophoresis coupled with probes specific

126 A direct comparison of SSDS with agarose gel electrophoresis for +/- screening shows that

127 capsids that migrated more slowly in native agarose gel electrophoresis from A36V mutant than from t

128 e liver using atomic force microscopy and 2D agarose gel electrophoresis in order to resolve this iss

129 the SDS-treated full-length particles during agarose gel electrophoresis is most likely caused by dis

130 In the current work, an improved agarose gel electrophoresis method for analysis of high

131 The widely used agarose gel electrophoresis method for assessing radiati

132 Agarose gel electrophoresis of DNA and RNA is routinely

133 over, DNA laddering was shown in myocytes by agarose gel electrophoresis of DNA fragments.

134 their retinas were evaluated by morphology, agarose gel electrophoresis of DNA, in situ terminal deo

135 onucleosomal DNA degradation was assessed by agarose gel electrophoresis of DNA, which showed DNA fra

136 d using classical morphological features and agarose gel electrophoresis of genomic DNA.

137 minimize errors and is broadly applicable to agarose gel electrophoresis of RNA samples and their sub

138 loci was determined by PCR amplification and agarose gel electrophoresis of the amplicons.

139 articles, were stable during purification by agarose gel electrophoresis or sucrose density gradient

140 An agarose gel electrophoresis pre-screening strategy ident

141 cles successfully separated by using a novel agarose gel electrophoresis procedure.

142 Agarose gel electrophoresis provided further biochemical

143 -screening non-sequence verified clones with agarose gel electrophoresis provides an inexpensive and

144 In addition, agarose gel electrophoresis revealed a 49% increase (P <

145 Marker validation by PCR and low-cost agarose gel electrophoresis revealed that 92.5% were pol

146 ild-type and mutant plants by single-nucleus agarose gel electrophoresis revealed that bleomycin-indu

147 Agarose gel electrophoresis revealed that KA (2.5 nmol)

148 Two-dimensional agarose gel electrophoresis revealed that ORC2 depletion

149 alysis of mammalian mtDNA by two-dimensional agarose gel electrophoresis revealed two classes of repl

150 We verified these AFM results by agarose gel electrophoresis separation of UV-irradiated

151 Agarose gel electrophoresis showed that high molecular w

152 d sequences were used in conjunction with an agarose gel electrophoresis system incorporating an AT-b

153 We show by two-dimensional (2D) agarose gel electrophoresis that replication forks natur

154 degrees C and 37 degrees C and shown by SDS-agarose gel electrophoresis to be comprised of a large,

155 mplified, and the amplicons were analyzed by agarose gel electrophoresis to determine the copy number

156 We used plasmid pBR322 and two-dimensional agarose gel electrophoresis to examine the collision of

157 genetics and high resolution two-dimensional agarose gel electrophoresis to examine the torsional ten

158 We apply agarose gel electrophoresis to sensitively evaluate prot

159 ation forks recover, we used two-dimensional agarose gel electrophoresis to show that replication-blo

160 Agarose gel electrophoresis was subsequently used to sep

161 Using two-dimensional agarose gel electrophoresis we also show that UvsW acts

162 om the present method and those derived from agarose gel electrophoresis were compared.

163 was more sensitive than conventional PCR and agarose gel electrophoresis with ultraviolet transillumi

164 SSRs are robust (with basic PCR methods and agarose gel electrophoresis), informative, and applicabl

165 cts from all of the reactions, visualized by agarose gel electrophoresis, allowed immediate identific

166 ed cells, using two-dimensional (2D) neutral agarose gel electrophoresis, and in a cell-free SV40 DNA

167 PCR amplification, agarose gel electrophoresis, and sequencing methods were

168 Agarose gel electrophoresis, circular dichroism and diff

169 analytical ultracentrifugation, quantitative agarose gel electrophoresis, electron cryomicroscopy, an

170 are based on two-dimensional, non-denaturing agarose gel electrophoresis, followed by structure deter

171 nnector or the procapsid, as investigated by agarose gel electrophoresis, SDS-PAGE, sucrose gradient

172 nation of TUNEL staining and pulse-field and agarose gel electrophoresis, suggesting a predominantly

173 ple criteria, including DNA fragmentation by agarose gel electrophoresis, terminal deoxynucleotidyltr

174 The amplified DNA was separated by agarose gel electrophoresis, transferred to nylon membra

175 Here, by native agarose gel electrophoresis, using recombinant IN with a

176 ragment length polymorphism (RFLP) typing by agarose gel electrophoresis, we compared the analyzer wi

177 Using two-dimensional agarose gel electrophoresis, we examined DNA replication

178 Using two-dimensional agarose gel electrophoresis, we show that mitochondrial

179 Using one- and two-dimensional agarose gel electrophoresis, we show that the linear mtD

180 Using 2D agarose gel electrophoresis, we show that the six o'cloc

181 ported by analysis of mtDNA molecules by 2-D agarose gel electrophoresis, which indicated the presenc

182 gut cells and DNA fragmentation analyses by agarose gel electrophoresis.

183 generate monovalent quantum dots (QDs) using agarose gel electrophoresis.

184 blot and DNA fragmentation was determined by agarose gel electrophoresis.

185 ry, terminal dUTP nick-end labeling, and DNA agarose gel electrophoresis.

186 SA and apo(a) size by Western blot after SDS-agarose gel electrophoresis.

187 light of new data using two-dimensional (2D) agarose gel electrophoresis.

188 rphisms were detected at 9 of the 11 loci by agarose gel electrophoresis.

189 ells was analyzed by native, two-dimensional agarose gel electrophoresis.

190 is beyond the resolution capacity of regular agarose gel electrophoresis.

191 om the amplification product of viral RNA by agarose gel electrophoresis.

192 nd the restriction fragments are resolved by agarose gel electrophoresis.

193 e last annealing cycle are separated by 4-6% agarose gel electrophoresis.

194 cies-specific fingerprints for comparison by agarose gel electrophoresis.

195 SA and apo(a) size by Western blot after SDS-agarose gel electrophoresis.

196 hese DNA fragments are typically analyzed by agarose gel electrophoresis.

197 leosomal DNA degradation was assessed by DNA agarose gel electrophoresis.

198 osomal fragmentation (ladder pattern) by DNA agarose gel electrophoresis.

199 quirement of 2 days to examine 96 samples by agarose gel electrophoresis.

200 size distributions using denaturing glyoxal-agarose gel electrophoresis.

201 dUTP terminal nick-end labeling assay and by agarose gel electrophoresis.

202 nt length polymorphisms (RFLPs) generated by agarose gel electrophoresis.

203 wed by amplifications with ISSR and SCoT and agarose gel electrophoresis.

204 nick end labeling (TUNEL) histochemistry and agarose gel electrophoresis.

205 as associated with DNA laddering as shown by agarose gel electrophoresis.

206 mplicons with sizes easily differentiated by agarose gel electrophoresis.

207 onfirmed by restriction enzyme digestion and agarose gel electrophoresis.

208 viability and the fragmentation of DNA using agarose gel electrophoresis.

209 ved with HindIII and HaeIII and subjected to agarose gel electrophoresis.

210 idence of DNA fragmentation when analyzed by agarose gel electrophoresis.

211 opoisomerase I (Topo I), and two-dimensional agarose gel electrophoresis.

212 gher sensitivity as compared to conventional agarose gel electrophoresis.

213 ng transmission electron microscopy and 1.2% agarose gel electrophoresis.

214 ugation, and retarded mobility during native agarose gel electrophoresis.

215 when positive, and results were verified by agarose gel electrophoresis.

216 ed, as revealed by semi-denaturing detergent agarose gel electrophoresis.

217 d transfer complex can be isolated by native agarose gel electrophoresis.

218 purifying DNA-origami nanostructures rely on agarose-gel electrophoresis (AGE) for separation.

219 d-UTP nick-end labeling (TUNEL) staining and agarose-gel electrophoresis of extracted slice DNA.

220 We have exploited the separation power of agarose-gel electrophoresis to purify milligram amounts

221 Agarose-gel electrophoresis was performed on all serum s

222 were isolated from the gel and reanalyzed by agarose-gel electrophoresis, single-nanoparticle-upconve

223 Using two-dimensional agarose-gel electrophoresis, we show that there is no sp

224 e-specific PCR qualitative assay followed by agarose-gel electrophoresis.

225 Based on a combination of two-dimensional agarose gel electrophoretic analysis and mapping of 5' e

226 Arabidopsis roots grown on inclined agarose gels exhibit a sinusoidal growth pattern known a

227 e pH change, measured using tissue-mimicking agarose gel, extends to 0.8 cm(3) in volume within an ho

228 medium model permitting the estimation of an agarose gel fiber radius and hydraulic permeability of t

229 ries of twofold dilutions of total DNA in an agarose gel followed by ethidium bromide staining, and s

230 rpo105-rpo273), followed by analysis on a 4% agarose gel for a 168-bp product.

231 lysis of the UsCPV genome segments (using 1% agarose gels) generated a migration pattern (electropher

232 Selective adsorption onto agarose gels has become a powerful method to separate si

233 its immobilization in low-weight-percentage agarose gels; however, fusion of CaM to MBP via a flexib

234 ng software package to automatically analyze agarose gel images of polymorphic DNA markers.

235 by bovine pancreatic trypsin immobilised on agarose gel in 100 mM ammonium hydrocarbonate buffer, pH

236 documented by DNA nick-end labeling, or DNA agarose gels in xenografts of human hematopoietic tumors

237 Aqueous agarose gels infused with 48 wt % NaClO3 at 6 degrees C,

238 In studies of in vitro agarose gel infusion, the use of functionalized Gd(3)N@C

239 rget binding functionality of CaM assayed in agarose gels is in good agreement with solution assays.

240 ing kinetics and compare the drying speed of agarose gels loaded with various non-gelling saccharides

241 the mobility of natural linear HA chains on agarose gels, making the complexes useful as defined siz

242 Immobilization in an agarose gel matrix eliminates potential interactions of

243 The YAC is maintained in an agarose gel matrix to avoid damage until the final steps

244 ined without the need of fluorescent probes, agarose gels, melting curves or sequencing analysis.

245 and only one-third the time compared to the agarose gel method.

246 orescence analysis of treponemes embedded in agarose gel microdroplets revealed that only minor porti

247 ylococcus aureus strains were deposited onto agarose gel modified electrodes which contained clinical

248 ings imply that the SSB yields inferred from agarose gels need reevaluation, especially when they wer

249 on fragment cloned from a band visible in an agarose gel of Pinus lambertiana (sugar pine) genomic DN

250 tall the extension of the acrosome bundle in agarose gels of different concentrations.

251 The reptation in agarose gels of H3/H4 tetramer arrays is essentially ind

252 tudy by in-situ interferometry the drying of agarose gels of various compositions cast in Petri dishe

253 %) and without contaminants such as residual agarose gel or DNA intercalating dyes.

254 these cultivars were killed by excess Al in agarose gels or in simple salt solutions.

255 sed interaction of proteoglycan with HCII in agarose gels paralleled increased activity in thrombin-H

256 oefficients for acetylcholine and choline in agarose gel perfused with physiological solutions were d

257 The computational results are validated with agarose gel phantom experiments.

258 n were preloaded with fluo-4, cast into a 1% agarose gel, placed above the compound sheets, and image

259 ssure-hypertrophied cats were embedded in an agarose gel, placed on a stretching device, and subjecte

260 purchased contained the desired cDNA clone, agarose gel pre-screening, colony isolation and similari

261 mical laser-induced nucleation of an aqueous agarose gel prepared with supersaturated potassium chlor

262 e restriction enzyme, HinfI, was run on 0.7% agarose gels, probed with radiolabeled (AATCCC)4, and ex

263 ted by the electrophoresis in polyacrylamide/agarose gel profile.

264 s measured, and the results suggest that the agarose gel reduces the effective supersaturation of the

265 Analysis of RIs on two-dimensional agarose gels revealed a rapid loss in the EBV bubble arc

266 SDS-agarose gels revealed small N2B (stiff) and large N2BA (

267 ent pH changes throughout a nanowire network/agarose gel sample during external solution pH changes,

268 ons were produced following infusions in six agarose gel samples at 2.4 T and from direct brain infus

269 ere analyzed by electrophoresis performed on agarose gel; samples with a discrete or localized band w

270 lly occurring X and M13 ssDNAs (as judged by agarose gel-shift assays and electron microscopic analys

271 A simple method for extracting DNA from agarose gel slices is described.

272 The agarose gel stabilizes the boundary between the leading

273 were separated by electrophoresis on a 0.8% agarose gel stained with ethidium bromide.

274 forms were produced and observed directly in agarose gels stained with Vistra Green and imaged with a

275 n these experiments, mice were given vaginal agarose gel suppositories containing either 5 mg OVA or

276 lts demonstrate that any modification to the agarose gel surface and, consequently, the permanent dip

277 A native agarose gel system was used to evaluate telomere DNA-bin

278 iffuse up to 2 orders of magnitude faster in agarose gel than their hard NP counterparts.

279 cted a new nucleoprotein complex on a native agarose gel that was produced in the presence of >200 nM

280 aging of arrays of qdots localized in dilute agarose gel, the blinking of qdots was measured across f

281 On agarose gels, the buffers in various concentrations were

282 al diameter, 0.579 mm) shallowly embedded in agarose gel, then to a collection reservoir.

283 Due to their migration in agarose gels, these incomplete physical forms of DNA hav

284 that saccharides systematically decrease the agarose gel thinning rate up to a factor two, and exempl

285 obleaching (FRAP) both in solution and in 2% agarose gels to compare transport properties of these ma

286 fragments were size separated on analytical agarose gels to create DNA fingerprints.

287 technique employs topographically patterned agarose gels to deliver various membrane preparations to

288 sensors in conjunction with collagen-coupled agarose gels to detect subcellular activities of SFK and

289 ted muscle fibers within biochemically inert agarose gels tuned to mimic native tissue stiffness.

290 ection of 10 muL cell inclusions in cm-sized agarose gels used here as phantom models of microtumors.

291 nanes that migrate in different bands on the agarose gels used to analyse the products of the reactio

292 solid materials including asphalt concrete, agarose gel, vaginal tissue, polymer, agar, bone, spider

293 n situ for 96 h to 200 microM total Al in an agarose gel was significantly less than that of cv Dade

294 To probe that question, U(IV) immobilized in agarose gels was exposed to conditions allowing biologic

295 Heterogeneity within agarose gels was modeled by assuming that fiber-rich, sp

296 domly distributed fluorescent nanospheres in agarose gel were obtained and fitted with the theoretica

297 electrophoresis and zone electrophoresis on agarose gel were used to monitor reaction conditions for

298 ctional blots to evaluate band modulation on agarose gels which are initially run to evaluate the rea

299 We use micropatterned, enzyme-laden agarose gels which are stamped on polyacrylamide films c

300 ns < or = 0.011) were covalently attached to agarose gels with volume fractions of 0.040 or 0.080.