ライフサイエンスコーパス: alanine substitution

1 ched from the body of the molecule by triple alanine substitution.

2 tamine substitutions (3-4-fold) than for the alanine substitution.

3 ability of Ser-282 to be phosphorylated via alanine substitution.

4 ylation site S2808 on RyR2 is inactivated by alanine substitution.

5 s of modeled YscO were targeted for multiple alanine substitutions.

6 estin mobilization, and internalization upon alanine substitutions.

7 ecific KDAMP was somewhat reduced by glycine-alanine substitutions.

8 ucture has been modified by multiple glycine/alanine substitutions.

9 three rec8 phospho-mutants, with 6, 24 or 29 alanine substitutions.

10 DNA binding in vitro of SpoIIID with single-alanine substitutions.

11 hosphorylation at Ser-318 as the Ser-318 --> alanine substitution abolishes the ability of the result

12 Cysteine-to-alanine substitution abrogated FAD-I's ability to induce

13 We performed alanine substitutions across the B-finger of human TFIIB

14 edented dataset of the effects of individual alanine substitutions across the E2 protein (355 positio

15 21 in the animal model, whereas glutamate or alanine substitution allowed tumor formation.

16 Six N-terminal alanine substitutions allowed ToxT transcriptional activ

17 In addition, single alanine substitutions along the sequence of P17-26 revea

18 of Klebsiella oxytoca NasR to site-directed alanine substitutions and measured RNA-binding activity.

19 IL-23 mutant differs from wild-type by five alanine substitutions and represents the dominant energe

20 to 70% of the wild-type level for all single alanine substitutions and the Y1188A/Y1191A protein.

21 OMPLA dimer interface by introducing single-alanine substitutions and used sedimentation equilibrium

22 s the anti-canonical nature of the Trp150 to alanine substitution, and also reveals a strong long dis

23 ild-type IC-B strain by a single arginine-to-alanine substitution at amino acid 533 of the attachment

24 A single alanine substitution at any of these decreases GCase bin

25 d repressor activities, using a ParA with an alanine substitution at Arg(351), a residue previously p

26 with wild-type GC or mutant GC containing an alanine substitution at Cys-122 (C122A).

27 We demonstrate that a single cysteine-to-alanine substitution at extracellular residue Cys-26, lo

28 type (WT) and CFP-NKA-alpha1 point mutants (alanine substitution at F956, E960, L964, and F967) for

29 t that knock-in mice harboring a cysteine-to-alanine substitution at Krt14's codon 373 (C373A) exhibi

30 An STMV CP variant having an arginine to alanine substitution at position 3 in the N-terminal 13a

31 n the maternal allele results in a valine to alanine substitution at residue 163 (p.Val163Ala).

32 A single alanine substitution at S986 reduced activation of an E2

33 Alanine substitution at Ser-204 and/or the neighboring S

34 tein kinase A (PKA) residues (i.e. serine to alanine substitution at Ser23/24; TnI(PKA-)) were bred w

35 ylatable MyBP-C PKA residues (i.e. serine to alanine substitution at Ser273, Ser282 and Ser302; MyBPC

36 A serine-to-alanine substitution at serine 118 also increases the cl

37 Here, we showed that alanine substitution at the A16 and A17 positions enhanc

38 Disruption of the motif by alanine substitution at the hydrophobic residues increas

39 e analysis of knock-in mice with cysteine-to-alanine substitution at the palmitoylated residues (4CA

40 is modification is critical to its function; alanine substitution at the phosphorylation sites blocks

41 An SFV mutant with an alanine substitution at this position (H3A) has a lower

42 Alanine substitution at this position in the JFH1 genome

43 Cepsilon phosphorylated NaV1.8 at S1452, and alanine substitution at this site blocked PKCepsilon mod

44 Mutants with an alanine substitution at yscU codon 263 displayed secreti

45 We analyzed alanine substitutions at 13 individual amino acids in FB

46 Except for P478A and K505A R508A, alanine substitutions at conserved residues abolished GP

47 Benign alanine substitutions at conserved surface residues elic

48 V > D39L > D39A > D39G approximately WT, and alanine substitutions at different sites, %S: N90A > S10

49 nt ASIC1a bearing truncation or glutamate-to-alanine substitutions at distal NT causes constitutive c

50 HLA-DR1 molecules with alanine substitutions at each of the six conserved H- bo

51 ion of astexin-2 and astexin-3 variants with alanine substitutions at each position within the ring a

52 rRST as a phosphorelay system, we introduced alanine substitutions at H1, D1, H2, and D2 and tested t

53 Conversely, non-phosphorylation-mimicking alanine substitutions at H273 and H327 relieved inhibiti

54 Alanine substitutions at HO-2 residues Leu-201 and Lys-1

55 ld-type FoxO1 but not FoxO1-AAA (mutant with alanine substitutions at known Akt phosphorylation sites

56 ing cognate, null, or "shaved" peptides with alanine substitutions at known TCR contact residues: Thr

57 combinant infectious SeV mutants with single alanine substitutions at L positions 1782, 1804, 1805, a

58 Alanine substitutions at L3 and L4 had no deleterious ef

59 genetics system, we analyzed the effects of alanine substitutions at many conserved residues within

60 in free energy of dimer association, whereas alanine substitutions at other interfacial positions had

61 the frequency of RNA recombination, whereas alanine substitutions at other sites in 3D(pol) increase

62 Alanine substitutions at Phe649 and Glu648, residues in

63 These data show that alanine substitutions at positions 2090, 2092, and 2093

64 and was deficient in a mutant K13 with three alanine substitutions at positions 58 to 60 (K13-58AAA)

65 The alanine substitutions at positions 90, 107, and 111, on

66 Alanine substitutions at positions Pro-113 Thr-115, Gly-

67 her the beta-flap tip or MotA is impaired by alanine substitutions at residues Leu-607, Arg-608, Phe-

68 Alanine substitutions at several positions within this r

69 IGF-1 rescue of AR toxicity is diminished by alanine substitutions at the Akt consensus sites.

70 Glycine to D-alanine substitutions at the carboxy termini can stabili

71 Alanine substitutions at the conserved positions have la

72 NA-PKcs(3A/3A) allele, which codes for three alanine substitutions at the mouse Thr2605 phosphorylati

73 Using the DNA-PKcs (5A) mouse model carrying alanine substitutions at the T2609 cluster, here we show

74 Previous analysis of leucine and alanine substitutions at the Val-65-equivalent site (Val

75 Alanine substitutions at these sites result in constitut

76 opB dimer interface demonstrated that single alanine substitutions at this critical interface signifi

77 Strains bearing a single or double serine to alanine substitutions at those sites were significantly

78 king the cytoplasmic tail and in tyrosine to alanine substitutions at Tyr-485 and Tyr-474.

79 V is critical for replication, with a single alanine substitution being sufficient to abrogate NLS fu

80 Alanine substitutions blocking Mn binding abrogated both

81 ue proline-rich motif are highly tolerant of alanine substitutions, but multiple substitutions that d

82 We found that alanine substitutions caused defects at both early and l

83 type and mutants) suggest that the serine-to-alanine substitution conferred reduced conductance with

84 ch both degrons were mutated by threonine to alanine substitutions (cyclin E(T74A T393A)) and report

85 In contrast, alanine substitutions decrease rapamycin resistance.

86 Alanine substitution decreased the agonist potency of Gl

87 However, a methionine-to-alanine substitution enables transport of calcium and ma

88 inally, with the exception of D206A, BLIP-II alanine substitutions exhibit a similar trend of effect

89 to neutralize pseudovirus containing single alanine substitutions exhibited a pattern distinct from

90 as inhibited but not completely prevented by alanine substitution for cysteine palmitoylation sites.

91 An alanine substitution for Ile-85 specifically interfered

92 Alanine substitution for proline had little impact on th

93 on mimic of the seven phosphorylation sites, alanine substitution for Ser(602), Thr(723), and/or Ser(

94 Single alanine substitutions for aromatic residues reduced entr

95 Single or paired alanine substitutions for Cys24 and Cys372 resulted in l

96 Alanine substitutions for LANA residues (1068)LKK(1070)

97 Alanine substitutions for one or two of the six aromatic

98 The N-terminal 13aa motif of the CP bearing alanine substitutions for positively charged residues lo

99 Single alanine substitutions for Ser-497, Thr-500, Ser-502, Ser

100 Alanine substitutions for solvent-inaccessible residues

101 Alanine substitutions for the first four or the last fou

102 Kinase-inactivating alanine substitutions for the invariant lysine in subdom

103 horylation-deficient PAP (PAP-7A) containing alanine substitutions for the seven phosphorylation site

104 is report shows that mutant proteins bearing alanine substitutions for two conserved arginines in a m

105 reas subtraction of these contacts by single alanine substitutions for Val131 or Val135 and glycine f

106 Extensive alanine substitutions had no effect on fusion triggering

107 Site-directed alanine substitution identified four residues of the IgE

108 Serine-to-alanine substitutions identified Ser-292/Ser-293, a site

109 The analogous lysine-to-alanine substitution in a glutamate-substituted phosphom

110 sion of VKOR, but not a mutant containing an alanine substitution in its conserved CXXC motif.

111 e perturb integrin function by a tyrosine-to-alanine substitution in membrane-proximal NPIY motif in

112 Sprouty1 knockin mice bearing a tyrosine-to-alanine substitution in position 53, corresponding to th

113 Most notably, a single serine-to-alanine substitution in the first of its two TM domains

114 us expressing an F protein with a glycine-to-alanine substitution in the fusion peptide (P/V-CPI(-)-G

115 ession of a construct expressing a serine-to-alanine substitution in the LAMMER kinase phosphorylatio

116 ting phosphorylation of the S936-TRP site by alanine substitution in transgenic Drosophila (trp(S936A

117 nd expressed mutant forms of UL53 containing alanine substitutions in a predicted helix.

118 SAS-6 mutants with alanine substitutions in a previously described ZYG-1 ta

119 Alanine substitutions in both UL50-S216 and UL53-S19 res

120 Three alanine substitutions in cluster H245/H304/H430 resulted

121 n-of-function to provide posttranscriptional alanine substitutions in eukaryotic proteins for potenti

122 spore inner membrane; (iii) shown that some alanine substitutions in GerBC significantly decrease th

123 McpB mutants having alanine substitutions in key arginine and tyrosine resid

124 sis of the cleavage reactions indicated that alanine substitutions in loop positions of these peptide

125 s S-nitrosylated, we made single cysteine-to-alanine substitutions in Panx1 (Panx1(C40A), Panx1(C346A

126 All recovered viruses with alanine substitutions in place of SWWS residues had seco

127 not formed with SpaA pilin mutants that have alanine substitutions in place of threonine in the LPXTG

128 Tat carrying alanine substitutions in the basic domain (AKKAAQAAA) re

129 a recombinant PEDV, KDKE(4A), with quadruple alanine substitutions in the catalytic tetrad of the 2'-

130 s of wild type and two mutants of MutS, with alanine substitutions in the conserved Phe-Xaa-Glu misma

131 The ability of F mutants with alanine substitutions in the CT to complement an F-null

132 Analyses of DCS mutants with alanine substitutions in the D(307)DTYD(311) and D(451)D

133 y their roles experimentally, we made single alanine substitutions in the human NaPi-IIa isoform and

134 Effects of five individual alanine substitutions in the prominent dimer groove did

135 e isolated recombinant viruses with specific alanine substitutions in the putative zinc finger motif

136 The 5A peptide with five alanine substitutions in the second helix had decreased

137 nd melittin Mut-2 (MM-2), possess leucine to alanine substitutions in the single and double heptadic

138 Consistent with these results, the alanine substitutions in the viral genome caused exagger

139 Most of the alanine substitutions in these residues exerted little e

140 uctural flexibility for activation of F, and alanine substitutions in this section, physical stress,

141 Mutant cycle analysis of alanine substitutions indicated that E153 and K196 are e

142 dues was necessary for fusion regulation, as alanine substitutions induced a 440% increase in fusion

143 We introduced alanine substitutions into each of these residues, expre

144 NV1 prME expression construct and found that alanine substitutions introduced to four highly conserve

145 Additional alanine substitutions involving residues in two or three

146 ures affecting PSM functions, we analyzed an alanine substitution library of PSMalpha3, a strongly cy

147 ling process because a YAP1 mutant harboring alanine substitutions (Mt-YAP5SA) in LATS1 kinase recogn

148 und regions of high similarity and performed alanine substitution mutagenesis to test the hypothesis

149 Using alanine substitution mutagenesis, we show that this regi

150 ical bundle (TM1-7) of the M3R to systematic alanine substitution mutagenesis.

151 atalytic functionality using structure-based alanine substitution mutagenesis.

152 cap-dependent translation, expression of an alanine substitution mutant 4E-BP1.S83A partially revers

153 A non-phosphorylatable alanine substitution mutant at this position causes enha

154 Utilizing a comprehensive alanine substitution mutant library of ToxT, 19 N-termin

155 Binding of both MAbs to gp41 alanine substitution mutant peptides required the DKW(66

156 Moreover, the triple alanine substitution mutant was unable to grow on transf

157 combinant proteins that are analogous to the alanine substitution mutants exhibit defects in nucleoti

158 Alanine substitution mutants in the deviant Walker A and

159 Functional analysis of alanine substitution mutants indicates that basic residu

160 Single alanine substitution mutants internalized iron at wild-t

161 mbly and budding, we constructed a series of alanine substitution mutants of M2 with mutations in the

162 Using deletion and alanine substitution mutants of RctB, we have now locali

163 Alanine substitution mutants of the G-H loop amino acids

164 Nine of 12 individual HeV G alanine substitution mutants possessed a complete defect

165 A screen of Saccharomyces cerevisiae histone alanine substitution mutants revealed that mutations in

166 quences in Ab-MLV transformation more fully, alanine substitution mutants that affect Mo-MLV replicat

167 nteraction, as evidenced by the inability of alanine substitution mutants to coimmunoprecipitate with

168 rprisingly, with only one exception (G105A), alanine substitution mutants with changes in residues af

169 ain and generated single, double, and triple alanine substitution mutants.

170 nd electrophysiology experiments on a set of alanine-substitution mutants confirmed functional roles

171 An alanine substitution mutation in a tyrosine-based motif

172 We generated a series of alanine substitution mutations at these residues in the

173 We identified nine alanine substitution mutations in 3D(pol) that resulted

174 f variants carrying a series of deletion and alanine substitution mutations in the carboxyl terminus

175 o test this, we engineered single and double alanine substitution mutations into the genome of murine

176 e contacts, we analyzed the effects of eight alanine substitution mutations on CheA-CheY binding inte

177 Furthermore, we reasoned that alanine substitution mutations that disrupt 3D(pol)-RNA

178 Aspartate or alanine substitution mutations throughout this region un

179 ular disulfide bonds using single and paired alanine substitution mutations.

180 By hierarchical assembly, 40 alanine-substitution mutations (covering all known modif

181 Alanine substitutions near or within these sequences wer

182 ct this crRNA size pattern and found that an alanine substitution of a conserved aspartate residue of

183 Alanine substitution of a glycine residue on the dimeriz

184 vity between PD81723 and NECA was reduced on alanine substitution of a number of ECL2 residues, inclu

185 Alanine substitution of a number of residues in the GIY-

186 We demonstrate that alanine substitution of a single threonine residue at po

187 Blocking MDM2 phosphorylation by alanine substitution of all six phosphorylation sites re

188 Alanine substitution of Arg-337 and Lys-338 showed that

189 Surprisingly, although alanine substitution of Asp-248 abolished manganese effl

190 tions of leucine and individual glycines and alanine substitution of both glycines within a LGYSG seq

191 Alanine substitution of C90, C97 or C103 in NsrR abrogat

192 Alanine substitution of conserved residues His42 in the

193 Alanine substitution of cysteine residues forming the C-

194 ow fluorescence annealing assays showed that alanine substitution of D9, E18 or E37 strengthened RNA

195 Alanine substitution of E171 (within the consensus motif

196 he TyrV:24 mutant could partly be rescued by alanine substitution of either AspIII:25 or GluVI:-06 in

197 Alanine substitution of Glu(68), Tyr(92), or Asn(139), w

198 The alanine substitution of individual amino acid residues o

199 Alanine substitution of individual hydrophobic amino aci

200 in central venous catheters in rats, whereas alanine substitution of K1595/R1596 in 1593FKKRFFKL1600

201 Alanine substitution of lysine residues K805/K806 in 804

202 Alanine substitution of PLB C-terminal residues signific

203 Alanine substitution of Q49 or N144 impeded the ability

204 Within this motif, triple alanine substitution of residues Leu(496), Leu(500), and

205 Alanine substitution of serine, threonine, and tyrosine

206 Alanine substitution of several residues in the RNA-cont

207 Alanine substitution of the conserved catalytic Cys-His

208 Disruption of the-fold by d-alanine substitution of the conserved central Gly(6)-Gln

209 Alanine substitution of the conserved residue H98 in prM

210 Alanine substitution of the hydrophobic residues I28, L3

211 Alanine substitution of the phosphorylation site Thr166

212 Alanine substitution of the RXL/Cy motif prevents this i

213 Alanine substitution of the SpaB lysine residue K139 or

214 Alanine substitution of the Ssd1 NLS prevents Ssd1 nucle

215 in SpaA harbors a disulfide bond in vivo and alanine substitution of these cysteines abrogates SpaA p

216 Alanine substitution of these ionizable residues decoupl

217 Alanine substitution of these leucines partially impaire

218 Alanine substitution of these residues (P4m) eliminated

219 We show that alanine substitution of these residues blocks the bindin

220 Alanine substitution of these three residues was suffici

221 Alanine substitution of Thr-759 renders DIAPH1 more stab

222 We now show that alanine substitution of tryptophan W2 within SCAMP2 E su

223 f phosphorylation to the shifted ligand, and alanine substitution of two residues (Glu-145 and Ser-14

224 rtion with the backbone carbonyl of Tyr-271; alanine substitution of Tyr-271, but not Phe-272, result

225 d receptor internalization was observed upon alanine substitution of TyrV:24.

226 d transgenic mice expressing an alpha1C with alanine substitutions of all conserved serine or threoni

227 study, we investigated the effects of single alanine substitutions of amino acid residues in the supp

228 Alanine substitutions of arginine 56 (R56A) and aspartic

229 increases in response to growth factors, and alanine substitutions of Arp2 T237 and T238 or Y202 inhi

230 Using the structure as a guide, alanine substitutions of basic residues in regions analo

231 The results showed that alanine substitutions of conserved residues W85 and Y113

232 Alanine substitutions of E1 S57 and E2 H170 destabilized

233 d the in vivo role of E3 in pH protection by alanine substitutions of E3 Y47 and Y48 (Y47/48A) in Sem

234 The same phenotype is observed with alanine substitutions of either the conserved cysteine o

235 MO7b or overexpression of mutated LMO7b with alanine substitutions of five potential JNK phosphorylat

236 Alanine substitutions of H348 and H352 inhibit virus gro

237 Alanine substitutions of key residues in the interface s

238 In contrast to the alanine substitutions of PQIIIS, the mutation of LQLP to

239 Alanine substitutions of predicted myristoylation (glyci

240 Alanine substitutions of residues within a region of E2

241 Alanine substitutions of S228 and M180 resulted in catal

242 The 3D(pol) NLS alanine substitutions of T19 and L21 results in aberrant

243 lls, but this distribution was unaffected by alanine substitutions of the arginine residues, which on

244 , and P(hybO), since IscR mutants containing alanine substitutions of the cysteine Fe-S ligands retai

245 t a 53BP1 phosphomutant, 53BP18A, comprising alanine substitutions of the eight most N-terminal S/TQ

246 Alanine substitutions of the hydrophobic residues in tho

247 Here, we identified seven single-alanine substitutions of the region 4 of sigma(70) (sigm

248 Alanine substitutions of these residues did not impair c

249 Alanine substitutions of Thr117 or Arg118 favor the reve

250 Individual alanine substitutions of two basic residues within the 4

251 ic flies expressing mutant dMTF-1 containing alanine substitutions of two, four or six cysteine resid

252 Alanine substitutions of tyrosines 118 and 148 at the ti

253 Most deletion mutations, but not alanine substitutions, of individual residues from posit

254 ructure loops, neither of which contains the alanine substitution, on both the dimerization and effec

255 Disrupting either PCI1 or PCI2 by alanine substitution or deletion diminishes CE associati

256 in((6-13)) and des-acyl ghrelin((6-13)) with alanine substitutions or cyclization, but not with d-ami

257 domain disrupted disintegration activity, an alanine substitution (P365A) in a conserved amino acid o

258 alpha-casein; VHLPP, alpha-zein) and the six alanine substitution peptides of PGTAVFK were synthesise

259 ation to aspartic acid blocked tethering and alanine substitution prevented mitotic Golgi unlinking.

260 well-studied common human genetic proline 12 alanine substitution (Pro12Ala) polymorphism.

261 These constructs focused on a single alanine substitution (R515A) and a truncation (M155n) at

262 S Delta19 proteins with other double alanine substitutions reduced cell-cell fusion further,

263 Four double alanine substitutions reduced entry to 5 to 10% of the w

264 nsitive version of PAH1 with a serine 162 to alanine substitution represses PC biosynthesis and also

265 phodeficient s15 carrying a threonine 136 to alanine substitution rescues dopamine neuron degeneratio

266 e graft-as dissected residue-by-residue with alanine substitutions-resembled more closely those of 2F

267 ntact residue, where canonical refers to the alanine substitution resulting in a matched change in th

268 Furthermore, alanine substitution revealed that the tryptophan in pos

269 Alanine substitutions revealed that the affinity changes

270 rse staurosporine effect on aspartate versus alanine substitutions reveals a cross-talk between diffe

271 Alanine substitution showed that GW/WG motifs in Delta12

272 ino acids 363 and 394 having four additional alanine substitutions (STANT + 7A) reduced desensitizati

273 In summary, the sensitivity of gp41 HR1 to alanine substitutions suggests that even subtle changes

274 The majority (13 of 15) of alanine substitutions supported yeast growth as the sole

275 non-phosphorylatable Ser282 (i.e. serine to alanine substitution, TG(S282A)).

276 ) that abrogated binding of ARF and a single alanine substitution that allowed ARF binding but inhibi

277 Previously identified alanine substitutions that can reduce AR3A binding to ex

278 he GP64 postfusion structure, we generated 2-alanine substitutions that scanned the two so-called fus

279 erB GR subunits in spores; and (iv) found no alanine substitutions that specifically affect interacti

280 The alanine substitution (that would promote a alpha-helical

281 Deletions or alanine substitutions throughout FUD caused loss of both

282 t the Asn-348-Tyr-427 interaction, and (iii) alanine substitutions throughout the region Phe-416-Pro-

283 We used alanine substitutions to examine the roles of all indivi

284 e, microspheres coated with JAM-A containing alanine substitutions to residues 43NNP45 (NNP-JAM-A) wi

285 Here we show that alanine substitutions to these residues also affect 3'-e

286 The extent of sensitivity of gp120-C5 to alanine substitutions underscores the importance of this

287 Consistently, a cysteine-to-alanine substitution was found in a reporter protein exp

288 In contrast to alanine substitution, we found that mutation of K96 to a

289 Using alanine substitutions, we identify several amino acid re

290 es for NS4B function in HCV RNA replication, alanine substitutions were engineered in place of 28 cha

291 stitution studies were employed, and several alanine substitutions were found to induce a partial ope

292 Four methionine substitutions and two alanine substitutions were introduced at fixed positions

293 sed, isolated A2 domain (bA2) variants where alanine substitutions were made for individual residues

294 Single and multiple alanine substitutions were made in these conserved resid

295 Serine-to-alanine substitutions were used to ablate the abilities

296 Alanine substitutions within a hydrophobic "neck" of the

297 Indeed, we identified multiple alanine substitutions within loop>J of the full length a

298 rotein import specifically, whereas specific alanine substitutions within the IBBL abrogated this act

299 We made alanine substitutions within the putative DNA binding he

300 Alanine substitutions within the WDXNWD motif abolish th