ライフサイエンスコーパス: alkyltransferase

1 by a suicide protein, O(6)-alkylguanine-DNA alkyltransferase.

2 n the DNA repair protein O6-alkylguanine-DNA alkyltransferase.

3 ay using monoclonal antibodies against human alkyltransferase.

4 tested for their ability to inactivate human alkyltransferase.

5 hways devoted to their repair, including DNA alkyltransferases.

6 ces the cysteine at the known active site of alkyltransferases.

7 an CblC exhibits glutathione (GSH)-dependent alkyltransferase activity and flavin-dependent reductive

8 In addition, O6-benzylguanine depleted alkyltransferase activity in BM cells at concentrations

9 These mutations had little effect on the alkyltransferase activity in repairing O6-methylguanine

10 This alkyltransferase activity is apparently catalyzed at a f

11 e, despite lacking the reactive cysteine and alkyltransferase activity of AGT.

12 xenografts displayed no O6-alkylguanine-DNA alkyltransferase activity, and their levels of glutathio

13 rtional to inhibition of O6-alkylguanine-DNA alkyltransferase activity, but a maximum tolerated dose

14 nitors were also found to have low levels of alkyltransferase activity.

15 nal enzyme with both glycoside hydrolase and alkyltransferase activity.

16 However, the Escherichia coli alkyltransferase, Ada-C, is not inactivated by O6-benzyl

17 d cytotoxic potential and is repaired by the alkyltransferase AGT.

18 r ovary (CHO) cells lack O6-alkylguanine-DNA alkyltransferase (AGT) activity and are sensitive to kil

19 rrow showed BG-resistant O6-alkylguanine-DNA-alkyltransferase (AGT) activity, and CFUs were stained i

20 f the DNA repair protein O6-alkylguanine-DNA alkyltransferase (AGT) and are sensitive to 1,3-bis(2-ch

21 f the DNA repair protein O6-alkylguanine-DNA-alkyltransferase (AGT) and increases rates of both spont

22 er chemotherapy target O(6)-alkylguanine-DNA alkyltransferase (AGT) and paradoxically protect cells f

23 h it is known that (i) O(6)-alkylguanine-DNA alkyltransferase (AGT) confers tumor cell resistance to

24 MT), also referred to as O6-alkylguanine-DNA alkyltransferase (AGT) correlate with the resistance of

25 the DNA repair enzyme O(6)-alkylguanine DNA alkyltransferase (AGT) from the O(6)-methylguanine methy

26 The DNA repair protein O6-alkylguanine-DNA alkyltransferase (AGT) has been shown to protect cells f

27 To evaluate the role of O6-alkylguanine-DNA alkyltransferase (AGT) in colon tumor chloroethylnitroso

28 O(6)-Alkylguanine-DNA alkyltransferase (AGT) is a DNA repair protein which rem

29 O6-alkylguanine-DNA alkyltransferase (AGT) is a DNA-repair protein that reve

30 O6-alkylguanine DNA alkyltransferase (AGT) is a key mechanism in the prevent

31 O(6)-alkylguanine-DNA alkyltransferase (AGT) is a single-cycle DNA repair enzy

32 O6-Alkylguanine-DNA alkyltransferase (AGT) is a suicide enzyme that repairs

33 O(6)-alkylguanine-DNA alkyltransferase (AGT) is a ubiquitous enzyme with an am

34 O6-Alkylguanine-DNA alkyltransferase (AGT) is an important cellular defense

35 O6-Alkylguanine-DNA alkyltransferase (AGT) is an important DNA repair protei

36 O6-Alklyguanine-DNA alkyltransferase (AGT) is an important DNA repair protei

37 the DNA repair protein O(6)-alkylguanine-DNA alkyltransferase (AGT) is an important source of tumor c

38 The DNA repair protein O(6)-alkylguanine DNA-alkyltransferase (AGT) is known to form DNA cross-links

39 The DNA repair protein O(6)-alkylguanine alkyltransferase (AGT) is responsible for removing promu

40 infusion that suppresses O6-alkylguanine-DNA alkyltransferase (AGT) levels in brain tumors, (2) evalu

41 Another way to deplete O6-alkylguanine DNA alkyltransferase (AGT) levels is to modify methylating a

42 Seventeen O(6)-alkylguanine-DNA alkyltransferase (AGT) mutants highly resistant to O(6)-

43 the DNA repair protein O(6)-alkylguanine-DNA alkyltransferase (AGT) paradoxically increases the mutag

44 O(6)-Alkylguanine-DNA alkyltransferase (AGT) plays a critical role in protecti

45 O(6)-Alkylguanine-DNA alkyltransferase (AGT) plays a major role in repair of t

46 O (6)-Alkylguanine-DNA alkyltransferase (AGT) plays an important role by protec

47 The DNA repair protein O6-alkylguanine-DNA alkyltransferase (AGT) protects cells from alkylation da

48 The activity of O6-alkylguanine-DNA alkyltransferase (AGT) protects cells from killing by me

49 The repair protein O(6)-alkylguanine-DNA alkyltransferase (AGT) protects cells from the mutagenic

50 Recombinant human O(6)-alkylguanine DNA alkyltransferase (AGT) protein or its variants (C145A an

51 O(6)-alkylguanine-DNA alkyltransferase (AGT) repairs damage to the human genom

52 O6-Alkylguanine-DNA alkyltransferase (AGT) repairs DNA by transferring the m

53 Human O6-alkylguanine-DNA alkyltransferase (AGT) repairs mutagenic O6-alkylguanine

54 The O6-alkylguanine-DNA alkyltransferase (AGT) repairs O6-alkylguanine and O4-al

55 ctive mutants of human O(6)-alkylguanine DNA alkyltransferase (AGT) show that it forms an O(6)-methyl

56 O6-alkylguanine-DNA alkyltransferase (AGT) specifically removes the methyl g

57 ance via production of O(6)-alkylguanine DNA alkyltransferase (AGT) thereby enabling yd T cell functi

58 ity of O(6)-benzylguanine (BG) to inactivate alkyltransferase (AGT) to potentiate the antitumor effic

59 ink the repair protein O(6)-alkylguanine-DNA alkyltransferase (AGT) to the N6 position of deoxyadenos

60 ligonucleotides by human O6-alkylguanine DNA alkyltransferase (AGT) were estimated using rapid reacti

61 active site domain of O(6)-alkylguanine-DNA alkyltransferase (AGT) with an endonuclease V domain.

62 uman DNA repair protein, O6-alkylguanine-DNA alkyltransferase (AGT), by O6-benzylguanine renders tumo

63 The DNA repair enzyme O(6)-alkylguanine DNA alkyltransferase (AGT), encoded by the gene MGMT, repair

64 O6alkylguanine-DNA alkyltransferase (AGT), encoded by the methylguanine met

65 the DNA repair protein, O6-alkylguanine-DNA alkyltransferase (AGT), has been shown to reduce nitroso

66 The DNA repair protein, O6-alkylguanine-DNA alkyltransferase (AGT), protects cells from the toxic an

67 the resistance protein O(6)-alkylguanine-DNA alkyltransferase (AGT), were synthesized and evaluated f

68 ical regulation of human O6-alkylguanine-DNA alkyltransferase (AGT), which determines the susceptibil

69 Human O(6)-alkylguanine-DNA alkyltransferase (AGT), which directly reverses endogeno

70 s the DNA repair protein O6-alkylguanine-DNA alkyltransferase (AGT), which removes chlorethylation or

71 the DNA repair protein O(6)-alkylguanine-DNA alkyltransferase (AGT), which removes chloroethylation o

72 istance to expression of O6-alkylguanine-DNA alkyltransferase (AGT), which repairs DNA damage caused

73 ells containing active O(6)-alkylguanine-DNA alkyltransferase (AGT), which repairs O(6)-meG, 3% misin

74 amines are repaired by O(6)-alkylguanine DNA alkyltransferase (AGT), which transfers the O(6)-alkyl g

75 e directly repaired by O(6)-alkylguanine-DNA alkyltransferase (AGT), which transfers the pyridyloxobu

76 itive factor (NSF) and O(6)-alklyguanine-DNA alkyltransferase (AGT), with moderate affinity (K approx

77 involvement of BRCA2 in O6-alkylguanine DNA alkyltransferase (AGT)-mediated repair of O6-methylguani

78 cy end point for overcoming alkylguanine DNA alkyltransferase (AGT)-mediated tumor cell resistance to

79 ated by the actions of O(6)-alkylguanine-DNA alkyltransferase (AGT).

80 e repair of O(6)-mG by O(6)-alkylguanine-DNA alkyltransferase (AGT).

81 the DNA-repair protein O(6)-alkylguanine-DNA alkyltransferase (AGT).

82 n all known sequences of O6-alkylguanine-DNA alkyltransferase (AGT).

83 atch repair activity and O6-alkylguanine-DNA alkyltransferase (AGT).

84 BCNU resistance protein, O6-alkylguanine DNA alkyltransferase (AGT).

85 ion recognition by the O(6)-alkylguanine DNA alkyltransferase (AGT).

86 agents are reduced by O(6)-alkylguanine-DNA alkyltransferase (AGT).

87 s demethylation by the O(6)-alkylguanine-DNA alkyltransferase (AGT).

88 rosslinks of DNA with O(6) -alkylguanine-DNA alkyltransferase (AGT, see picture).

89 ir proteins related to O(6)-alkylguanine-DNA alkyltransferases (AGTs) that tightly bind alkylated DNA

90 human DNA repair protein O6-alkylguanine-DNA alkyltransferase (alkyltransferase) in vitro than O6-ben

91 O6-Alkylguanine-DNA alkyltransferase (alkyltransferase) provides an importan

92 ive as inactivators of O(6)-alkylguanine-DNA alkyltransferase (alkyltransferase) than either O(6)-ben

93 ctural conformations related to the distinct alkyltransferase and acetyltransferase reactions catalyz

94 than O6-benzylguanine against the wild-type alkyltransferase and is even capable of inactivating the

95 it can be repaired by O(6)-alkylguanine DNA alkyltransferase and mismatch repair pathway.

96 inding proteins, agt for an alkyl-cysteine-S-alkyltransferase and Taf1 for a subunit of transcription

97 ery slow, whereas the E. coli Ogt, the human alkyltransferase, and the mutant A316P/W336A-Ada-C alkyl

98 Determination of O6-alkylguanine-DNA-alkyltransferase (AT) activity revealed that astrocytes

99 Depletion of O6-alkylguanine-DNA-alkyltransferase (ATase) activity confers only limited i

100 A human O6-alkylguanine-DNA-alkyltransferase (ATase) cDNA-containing retrovirus was

101 O6-BeG) on the levels of O6-alkylguanine-DNA alkyltransferase (ATase) in the hematopoietic compartmen

102 The DNA repair protein, O6-alkylguanine-DNA alkyltransferase (ATase) is an important cellular resist

103 The expression of O6-alkylguanine-DNA-alkyltransferase (ATase), which is responsible for repai

104 Inactivation of O6-alkylguanine-DNA alkyltransferase by O6-benzylguanine renders tumor cells

105 Inactivation of alkyltransferase by the gamma-folate ester of O6-[4-(hyd

106 ersible inactivator of O(6)-alkylguanine-DNA alkyltransferase currently in clinical trials to overcom

107 V139F provided alkyltransferase-deficient bacteria with greater protect

108 rary was selected for the ability to provide alkyltransferase-deficient Escherichia coli with resista

109 lkyl-accepting cysteine was transformed into alkyltransferase-deficient Escherichia coli.

110 dence supports the existence of at least two alkyltransferase-dependent pathways for 1,2-dibromoethan

111 est that the enzyme belongs to the family of alkyltransferase enzymes for which Zn plays a key role i

112 est that the enzyme belongs to the family of alkyltransferase enzymes for which Zn plays a role in ac

113 here in vitro and in vivo studies on the DNA alkyltransferase from the thermophilic archaeon Sulfolob

114 olymorphism in the human O6-alkylguanine-DNA alkyltransferase gene exists, with about 15% of the popu

115 Patients with high levels of alkyltransferase had shorter time to treatment failure (

116 y of recombinant human O(6)-alkylguanine-DNA alkyltransferase (hAGT) revealed a previously unknown zi

117 site cysteine of human O(6)-alkylguanine-DNA alkyltransferase (hAGT), Cys145, was shown to be highly

118 ssion of human protein O(6)-alkylguanine-DNA alkyltransferase (hAGT), in which the active site cystei

119 f the DNA repair protein O6-alkylguanine-DNA alkyltransferase in brain tumors was correlated with res

120 The level of alkyltransferase in CD34+ cells or in mononuclear BM cel

121 A high level of the alkyltransferase in many tumour cells renders them resis

122 rotein O6-alkylguanine-DNA alkyltransferase (alkyltransferase) in vitro than O6-benzylguanine, the pr

123 ise as an agent for possible tumor-selective alkyltransferase inactivation, which suggests it may pro

124 n vitro than O6-benzylguanine, the prototype alkyltransferase inactivator currently in clinical trial

125 provide a new class of highly water-soluble alkyltransferase inactivators and form the basis to cons

126 O6-benzyl-2'-deoxyguanosine were more potent alkyltransferase inactivators than the parent nucleoside

127 t the DNA repair protein O6-alkylguanine-DNA alkyltransferase increases the mutagenicity of 1,2-dibro

128 wer in the cells lacking O6-alkylguanine-DNA alkyltransferase, indicating that O6-MeG was causally in

129 n with dual-dose O6-benzylguanine to prolong alkyltransferase inhibition could reach an MTD.

130 ustine (BCNU) plus the O(6)-alkylguanine-DNA alkyltransferase inhibitor O(6)-benzylguanine (O(6)-BG)

131 lguanine and that higher doses or additional alkyltransferase inhibitors capable of inactivating this

132 In order to produce more soluble alkyltransferase inhibitors, we have synthesized three a

133 The human alkyltransferase is inactivated by the free base O6-benz

134 The alkylated form of DNA alkyltransferases is inactive, and in eukaryotes, it is

135 ne (BG), an inhibitor of O6-alkylguanine-DNA alkyltransferase, is being tested clinically for its abi

136 illing of tumors that express high levels of alkyltransferase, it would also be expected to reduce th

137 horionic gonadotropin-O(6) -alkylguanine-DNA alkyltransferase) led to LAMP-to-hCG signal transduction

138 ld encourage prospective studies to evaluate alkyltransferase levels as a method, for identifying bra

139 Alkyltransferase levels in individual cells in sections

140 d also be expected to reduce the already low alkyltransferase levels of hematopoietic stem cells and,

141 , survival was more strongly correlated with alkyltransferase levels than with age.

142 er of importance, were age, tumor grade, and alkyltransferase levels.

143 ate 1.7 times greater than patients with low alkyltransferase levels.

144 The alkyltransferase-like (ATL) proteins contain primary seq

145 Alkyltransferase-like (ATL) proteins in Schizosaccharomy

146 reveal the presence of a group of proteins [alkyltransferase-like (ATL) proteins] showing amino acid

147 ccharomyces pombe a DNA recognition protein, alkyltransferase-like 1 (Atl1), can play a pivotal role

148 Alkyltransferase-like enzymes mark O(6)-alkylguanine les

149 R process in those organisms that express an alkyltransferase-like gene and raise the question of whe

150 Alkyltransferase-like proteins (ATLs) are a novel class

151 Alkyltransferase-like proteins (ATLs) share functional m

152 Additionally, alkyltransferase-like proteins (ATLs), which are structu

153 O6-alkylguanine DNA-alkyltransferase mediated DNA repair effectively blocks

154 The alkyltransferase-mediated mutations produced by 1,2-dibr

155 ase currently in clinical trials to overcome alkyltransferase-mediated resistance to certain cancer c

156 igenetic regulation of O(6)-alkylguanine DNA alkyltransferase (MGMT) is surrogate of intrinsic resist

157 O(6)-Alkylguanine-DNA alkyltransferase (MGMT) is the sole repair protein for O

158 Human O(6)-alkylguanine-DNA alkyltransferase (MGMT) repairs potentially cytotoxic an

159 bited the transfer of methyl groups to human alkyltransferase (MGMT).

160 s in DNA are repaired by O6-alkylguanine-DNA alkyltransferases (MGMT) by transfer of the alkyl group

161 D34+) hematopoietic precursors do not induce alkyltransferase, myelosuppression may be the dose-limit

162 cell function include O(6)-alkylguanine DNA alkyltransferase, nucleotide excision repair, base excis

163 Unlike the Escherichia coli alkyltransferase Ogt that also repairs O(4)-methylthymin

164 e translocation of the O(6)-alkylguanine DNA alkyltransferase on DNA, reaching single base-pair resol

165 lamin intermediate via glutathione-dependent alkyltransferase or reductive elimination activities.

166 This mutant human alkyltransferase, or others similarly created and select

167 SNAP or CLIP forms of O(6)-alkylguanine-DNA-alkyltransferase plus a peptide epitope tag.

168 em cells with a gene encoding a BG-resistant alkyltransferase prior to BG/alkylation treatment.

169 DNA repair alkyltransferases protect organisms against the cytotoxi

170 ine lesions that are poor substrates for the alkyltransferase proteins in higher eukaryotes might, by

171 se found in DNA repair O(6)-alkylguanine-DNA alkyltransferase proteins.

172 olecular modeling of their interactions with alkyltransferase provided a molecular explanation for th

173 are better substrates than methyl groups for alkyltransferases provided that steric factors do not pr

174 O6-Alkylguanine-DNA alkyltransferase (alkyltransferase) provides an important source of resist

175 ransferase, and the mutant A316P/W336A-Ada-C alkyltransferases reacted very rapidly with this 16-mer

176 previous proposal that AANAT can catalyze an alkyltransferase reaction in a conformationally altered

177 The alkyltransferase removes the alkyl group to one of its o

178 A repair protein human O(6)-alkylguanine-DNA alkyltransferase repairs lesions at the 5' ends of 70-nu

179 genicity, one involving Gua N7-alkylation by alkyltransferase-S-CH2CH2Br and depurination, plus anoth

180 s of full-length human O(6)-alkylguanine-DNA alkyltransferase (SNAP-tag).

181 e we report the X-ray structure of the human alkyltransferase solved using the technique of multiple

182 effective in preventing mutations than human alkyltransferase, suggesting that the endonuclease V act

183 s of O(6)-alkylguanine-DNA alkyltransferase (alkyltransferase) than either O(6)-benzylguanine or O(6)

184 ven capable of inactivating the P140K mutant alkyltransferase that is resistant to inactivation by O6

185 A pool of 6.5 x 10(6) human alkyltransferases that were randomly mutated at six amin

186 to interpret the behaviour of certain mutant alkyltransferases to enhance biochemical understanding o

187 d in epoxide carboxylation: a zinc-dependent alkyltransferase, two short-chain dehydrogenases with sp

188 e subpopulation of cells with high levels of alkyltransferase was correlated directly with drug resis

189 ffering only in level of O6-alkylguanine-DNA alkyltransferase, were treated with MNU and assayed for

190 ta-position greatly enhances inactivation of alkyltransferase, whereas para-substitution has little e

191 ng for a mutant version of O(6)-alkylguanine alkyltransferase, which is efficiently assembled with an

192 the DNA repair protein, O6-alkylguanine-DNA alkyltransferase, which may explain their susceptibility

193 ors capable of inactivating this form of the alkyltransferase will be necessary.

194 cubation of Escherichia coli-expressed human alkyltransferase with 1,2-dibromoethane and single-stran

195 phan, or alanine on the interaction of human alkyltransferase with O6-benzylguanine using direct dete