ライフサイエンスコーパス: alpha-mannosidase

1 y an alternate pathway comprising a distinct alpha-mannosidase.

2 tructural and mechanistic dissection of endo-alpha-mannosidase.

3 nalysis revealed that 2DG-ODDA is cleaved by alpha-mannosidases.

4 ues for human lysosomal and Drosophila Golgi alpha-mannosidases.

5 which in turn induces expression of secreted alpha-mannosidases.

6 --> (1)S5 conformational itinerary for GH125 alpha-mannosidases.

7 No alpha-mannosidase activity could be detected in Pichia t

8 annosidase cDNA immunoprecipitated lysosomal alpha-mannosidase activity from human fibroblast extract

9 1 orthologs, respectively, each of which had alpha-mannosidase activity in vitro.

10 consequences caused by the lack of cytosolic alpha-mannosidase activity in vivo by the generation of

11 reliable continuous assay for measurement of alpha-mannosidase activity is described and demonstrated

12 reports of this enzyme indicated that it has alpha-mannosidase activity, however, we conclusively sho

13 ed by 1-deoxymannojirimycin, an inhibitor of alpha-mannosidase activity, without affecting the monome

14 alpha-mannosidase (alpha-Man; EC 3.2.1.24) and beta-D-N-

15 alpha-Mannosidase (Ams1) is another resident hydrolase t

16 cuolar enzymes, aminopeptidase I (Ape1p) and alpha-mannosidase (Ams1p), to the vacuole.

17 ypical LSD caused by deficiency of lysosomal alpha-mannosidase and characterized by cerebellar ataxia

18 lysosomal glycosidases alpha-galactosidase, alpha-mannosidase and neuraminidase.

19 ent of the G protein with the exoglycosidase alpha-mannosidase and reduced after subsequent treatment

20 cDNA sequences encoding a putative lysosomal alpha-mannosidase and several differences were found rel

21 tion of the antigen oligomannoside moiety by alpha-mannosidase and that CD1e is an accessory protein

22 the catalytic properties of the Sf9 class II alpha-mannosidase and to more clearly determine its rela

23 nally distinct glycoside hydrolase 38 (GH38) alpha-mannosidases and a specific GH125 alpha-1,6-mannos

24 alpha-Mannosidases and alpha-mannanases have attracted a

25 a-glucosidase, beta-glucosidase, isomaltase, alpha-mannosidase, and glucoamylase, were obtained.

26 Although ubiquitously expressed, alpha-mannosidases are targeted to lysosomes or vacuoles

27 y whose cargo proteins, aminopeptidase I and alpha-mannosidase, are selectively transported from the

28 although lvsB mutants inefficiently retained alpha-mannosidase, as well as two other lysosomal cystei

29 This enzyme is not a lysosomal alpha-mannosidase because it is not active at acidic pH

30 igned catalytic itinerary is that of exo-1,6-alpha-mannosidases belonging to CAZy family 125.

31 n demonstrated that other mammalian class II alpha-mannosidases can participate in N-glycan processin

32 elected residues map to the periphery of the alpha-mannosidase catalytic domain tertiary structure.

33 aised to the recombinant product of the 3-kb alpha-mannosidase cDNA immunoprecipitated lysosomal alph

34 One unusual enzyme, endo-alpha-mannosidase, cleaves mannoside linkages internally

35 Class I alpha-mannosidases comprise a homologous and functionall

36 assayed, and in many studies, the numbers of alpha-mannosidase-containing cells were enumerated.

37 CD1e selectively assists the alpha-mannosidase-dependent digestion of PIM(6) species

38 performed using recombinant Drosophila Golgi alpha-mannosidase (dGMII) has been shown to give the kin

39 d in Mycobacterium smegmatis and digested by alpha-mannosidase, did not activate T cells.

40 analysis of released N-glycans and jack bean alpha-mannosidase-digested glycopeptides revealed the pr

41 metry, N-terminal amino acid sequencing, and alpha-mannosidase digestion demonstrated universal O gly

42 and protease treatments but was destroyed by alpha-mannosidase digestion.

43 essed two cDNAs encoding the human lysosomal alpha-mannosidase (EC 3.2.1.24) by RT-PCR of human splee

44 n addition, we have identified three class I alpha-mannosidases, EDEM1, EDEM2, and ERManI, which play

45 e found relative to the functional lysosomal alpha-mannosidase encoded by the 3-kb spleen cDNA.

46 at room temperature of LAM-spiked urine with alpha-mannosidase (for human TB), and LAM-spiked milk wi

47 tructures revealed that bifidobacterial GH38 alpha-mannosidases form homotetramers, with the N-termin

48 enabled activity-based protein profiling of alpha-mannosidases from both human cell lysate and mouse

49 aMan) and the biologically relevant class II alpha-mannosidases from Drosophila melanogaster belongin

50 underlying these traits as well as a single alpha-mannosidase gene directly associated with this tol

51 or inhibition of human endoplasmic reticulum alpha-mannosidase I (ER Man I) and mouse Golgi alpha-man

52 idases, including endoplasmic reticulum (ER) alpha-mannosidase I (ERManI) and Golgi alpha-mannosidase

53 The Arabidopsis ER-alpha-mannosidase I (MNS3) generates an oligomannosidic

54 volves selective trimming of N-glycans by ER alpha-mannosidase I and subsequent recognition by the ER

55 ns attached to misfolded glycoproteins by ER alpha-mannosidase I and subsequent recognition by the ER

56 es of wild type and mutant forms of human ER alpha-mannosidase I as well as by structural analysis of

57 haracterized by down-regulation of the Golgi alpha-mannosidase I coding gene MAN1A1, leading to eleva

58 quent reshaping of the glycome by inhibiting alpha-mannosidase I resulted in significantly higher mig

59 Comparative studies with ER alpha-mannosidase I show a different mechanism, where to

60 ely Arabidopsis (Arabidopsis thaliana) Golgi alpha-mannosidase I, Nicotiana tabacum beta1,2-N-acetylg

61 is of wild type and mutant forms of human ER alpha-mannosidase I.

62 in CHO cells cultured in the presence of the alpha-mannosidase-I inhibitor kifunensine.

63 (ER) alpha-mannosidase I (ERManI) and Golgi alpha-mannosidase IA (GMIA), are responsible for cleavag

64 pha-mannosidase I (ER Man I) and mouse Golgi alpha-mannosidase IA (Golgi Man IA).

65 In contrast to the classical Golgi alpha-mannosidase II (AMAN-2), AMAN-3 displayed a cobalt

66 of mannose trimming enzyme drosophila Golgi alpha-mannosidase II (dGMII) complexed with the inhibito

67 ations characterized included inserts in the alpha-mannosidase II (dGMII), ash1, and pumilio genes.

68 Golgi alpha-mannosidase II (GMII), a member of glycoside hydro

69 glucosaminyltransferase I, Arabidopsis Golgi alpha-mannosidase II (GMII), and Arabidopsis beta1,2-xyl

70 Inhibition of Golgi alpha-mannosidase II (GMII), which acts late in the N-gl

71 ized and cosedimented with the Golgi marker, alpha-mannosidase II (Man II).

72 enous GalT was compared with transfected rat alpha-mannosidase II (medial-Golgi, polyclonal antibody)

73 alpha-Mannosidase II (MII) is a key enzyme converting pr

74 ormal development consistent with increasing alpha-mannosidase II and core fucosyl-transferase enzyme

75 ated a cDNA encoding a protein homologous to alpha-mannosidase II and designated it alpha-mannosidase

76 ity, Green et al. now show that mice lacking alpha-mannosidase II develop an autoimmune disease simil

77 ite of glycan M5G0 upon binding to the Golgi alpha-mannosidase II enzyme.

78 e II, alpha-mannosidase IIx colocalizes with alpha-mannosidase II in COS cells.

79 Golgi alpha-mannosidase II is an enzyme that processes the int

80 ttering kinetics was seen with the HeLa GalT/alpha-mannosidase II pairing.

81 sion consisting of the first 117 residues of alpha-mannosidase II tagged with a fluorescent protein a

82 Swainsonine, an inhibitor of Golgi alpha-mannosidase II that causes abnormal N-glycosylatio

83 This staining pattern was similar to that of alpha-mannosidase II which is a known resident enzyme of

84 NBCCV was found to be colocalized with alpha-mannosidase II, a marker for the Golgi complex.

85 When coexpressed with alpha-mannosidase II, alpha-mannosidase IIx colocalizes

86 Swainsonine, an inhibitor of Golgi alpha-mannosidase II, blocked beta1,6GlcNAc N-glycan exp

87 rkers of the RER (ribophorin I) and GA (p58, alpha-mannosidase II, galactosyltransferase, and TGN38/4

88 In contrast, rab6 and alpha-mannosidase II, Golgi marker proteins, appear unch

89 how that mutation of a single gene, encoding alpha-mannosidase II, which regulates the hybrid to comp

90 alpha-Mannosidase II-deficient autoimmune disease is due

91 etermine its relationship to mammalian Golgi alpha-mannosidase II.

92 mes colocalized with the medial-Golgi marker alpha-mannosidase II.

93 biochemical similarities to mammalian Golgi alpha-mannosidase II.

94 h that the Sf9 enzyme is distinct from Golgi alpha-mannosidase II.

95 Alpha-mannosidase-II (alphaM-II) catalyzes the first com

96 Alpha-mannosidase-II (alphaM-II) deficiency diminishes c

97 ntra-Golgi transport of Rh1, downstream from alpha-mannosidase-II in the medial- Golgi.

98 se properties, we designated this enzyme Sf9 alpha-mannosidase III and concluded that it probably pro

99 Because alpha-mannosidase IIx (MX) is a candidate enzyme for thi

100 spermatozoa, a phenotype similar to that of alpha-mannosidase IIx (MX) KO mice.

101 rgeted disruption of Man2a2, a gene encoding alpha-mannosidase IIx (MX), an enzyme that forms interme

102 When coexpressed with alpha-mannosidase II, alpha-mannosidase IIx colocalizes with alpha-mannosidase

103 protein A fusion of the catalytic domain of alpha-mannosidase IIx hydrolyzes a synthetic substrate,

104 The results suggest that alpha-mannosidase IIx hydrolyzes two peripheral Man alph

105 Here, we show by immunocytochemistry that alpha-mannosidase IIx resides in the Golgi in HeLa cells

106 d Chinese hamster ovary cells overexpressing alpha-mannosidase IIx show a reduction of M(6)Gn(2) and

107 us to alpha-mannosidase II and designated it alpha-mannosidase IIx.

108 markably, ER mannosidase I/Man1b1, the first alpha-mannosidase implicated in this conventional N-glyc

109 ted a function for maternally deposited acid alpha-mannosidase in yolk consumption.

110 ultiple glycoside hydrolase family 47 (GH47) alpha-mannosidases, including endoplasmic reticulum (ER)

111 Complexes with the established endo-alpha-mannosidase inhibitor alpha-Glc-1,3-deoxymannonoji

112 A practical synthesis of the potent class I alpha-mannosidase inhibitor kifunensine (1) beginning fr

113 ain in a CHO cell line in the presence of an alpha-mannosidase inhibitor kifunensine, and an endoglyc

114 ild-type KOR1 in the presence of the class I alpha-mannosidase inhibitor kifunensine, which abolished

115 ay using several concentrations of the known alpha-mannosidase inhibitor swainsonine are also present

116 Treatment of zebrafish embryos with the alpha-mannosidase inhibitor swainsonine resulted in the

117 Compounds 15 and 16 were specific alpha-mannosidase inhibitors, and 24 and 26 were potent

118 n ligand, (ii) treatment of amastigotes with alpha-mannosidase inhibits the binding of mannose-bindin

119 annose upper arm, indicating that a specific alpha-mannosidase is required to generate substrates for

120 anel of glycosidases including the Jack Bean alpha-mannosidase (JBalphaMan) and the biologically rele

121 luated clinically and pathologically, tissue alpha-mannosidase levels were assayed, and in many studi

122 [endoplasmic reticulum degradation enhancing alpha-mannosidase like protein 2]).

123 EDEM1 (ER degradation-enhancing alpha-mannosidase-like 1 protein) has been proposed to p

124 ck, and upregulates ER degradation-enhancing alpha-mannosidase-like protein (EDEM) and ER chaperones,

125 (endoplasmic reticulum degradation-enhancing alpha-mannosidase-like protein 2) regulation of cardiac

126 s genomes predicted ER degradation-enhancing alpha-mannosidase-like protein and Mns1 orthologs, respe

127 recognition by the ER degradation-enhancing alpha-mannosidase-like protein family of lectins, both m

128 recognition by the ER degradation-enhancing alpha-mannosidase-like protein family of lectins, both m

129 endoplasmic reticulum degradation-enhancing alpha-mannosidase-like protein mRNA levels were inversel

130 regulation of EDEM (ER degradation-enhancing alpha-mannosidase-like protein) also suppressed the degr

131 induction of EDEM (ER degradation-enhancing alpha-mannosidase-like protein).

132 1 target gene EDEM (ER degradation-enhancing alpha-mannosidase-like protein, a protein degradation fa

133 s, including ERdj4, ER degradation-enhancing alpha-mannosidase-like protein, and p58(IPK), or express

134 ERAD) through upregulation of ERAD-enhancing alpha-mannosidase-like proteins (EDEMs) protected agains

135 dase-like proteins (ER degradation-enhancing alpha-mannosidase-like proteins 1, 2, and 3) are part of

136 alpha-mannosidase ManIIb (GM) and lysosomal alpha-mannosidase LManII (LM).

137 ses, including a broad specificity lysosomal alpha-mannosidase (LysMan), core-specific alpha1,6-manno

138 m N-glycan processing gene arrays identified alpha-mannosidases (MAN1A2 and MAN1C1) as targets for do

139 obacco (Nicotiana tabacum) plants of a human alpha-mannosidase, MAN2B1, which is a lysosomal enzyme i

140 alpha-Mannosidase (MAN2C1) is the enzyme responsible for

141 ng to glycohydrolase family 38, namely Golgi alpha-mannosidase ManIIb (GM) and lysosomal alpha-mannos

142 han lung function, and that genes related to alpha-mannosidase may influence risk of emphysema.

143 ted the role of Arabidopsis thaliana class I alpha-mannosidases (MNS1 to MNS5) in glycan-dependent ER

144 However, overexpression of the alpha-mannosidase MNS4 was shown to overcome lectin-depe

145 vage of the beta-1,2-xylose, followed by the alpha-mannosidase NixJ (GH125), which removes the alpha-

146 The removal of the alpha-1,3-mannose by the alpha-mannosidase NixK (GH92) is a prerequisite for the

147 cell lines processed and targeted lysosomal alpha-mannosidase normally, indicating the lack of a sig

148 nt percentage of newly synthesized lysosomal alpha-mannosidase precursor polypeptides.

149 ic locus for upper-lower lobe ratio near the alpha-mannosidase-related gene MAN2B1 (rs10411619; P = 1

150 mong African Americans, a locus near a third alpha-mannosidase-related gene, MAN1C1 (rs12130495; P =

151 Our data demonstrated that AMAN-3 is a Golgi alpha-mannosidase required for core fucosylation of the

152 alignment of the catalytic domain of class I alpha-mannosidases reveals four well-supported phylogene

153 long the reaction coordinate of an inverting alpha-mannosidase show how the enzyme distorts the subst

154 ound to be less potent inhibitors of Class I alpha-mannosidases than kifuensine itself, the bis(hydro

155 zyme from all other known mammalian class II alpha-mannosidases that can hydrolyze Man(5)GlcNAc(2).

156 A swainsonine-sensitive alpha-mannosidase trims some N-glycans to biantennary Ma

157 wever, the majority of the newly synthesized alpha-mannosidase was transported with normal kinetics a

158 e sorting efficiency of the lysosomal enzyme alpha-mannosidase were normal in the mutant strain.

159 and the efficiency of targeting of lysosomal alpha-mannosidase were normal, although lvsB mutants ine

160 Three class I alpha-mannosidases were identified to play a critical ro

161 ux experiments, Rab7 T22N cells oversecreted alpha-mannosidase, whereas Rab7 WT cells retained this h

162 otein (GFP) fusion protein co-localized with alpha-mannosidase, which indicated that the fusion prote

163 n insect (Sf9) cell cDNA encoding a class II alpha-mannosidase with amino acid sequence and biochemic

164 of a novel human glycosylhydrolase family 38 alpha-mannosidase with catalytic characteristics similar

165 pecificity studies comparing the novel human alpha-mannosidase with human LysMan revealed that the fo