ライフサイエンスコーパス: alpha-tubulin

1 o fluorescently label an endogenous protein (alpha-tubulin).

2 ent on posttranslational "detyrosination" of alpha-tubulin.

3 ic clash of the compound with the T5-loop of alpha-tubulin.

4 GT1-7 cells with induction in acetylation of alpha-tubulin.

5 stone H4 acetylated at K16 (Ac-H4K16) and Ac-alpha-tubulin.

6 oximity to the lysine 40 acetylation site in alpha-tubulin.

7 L1/2, an antibody classically used to detect alpha-tubulin.

8 n bend that predicts a novel engagement with alpha-tubulin.

9 rgets include histone H4 lysine 16, p53, and alpha-tubulin.

10 ranslational retyrosination of detyrosinated alpha-tubulin.

11 IM1 and Orai1, accompanied by hypoacetylated alpha-tubulin.

12 erent post-translationally modified forms of alpha-tubulin.

13 ncer cells was accompanied by hypoacetylated alpha-tubulin.

14 ed cell motility is through deacetylation of alpha-tubulin.

15 lation of its cytoplasmic substrates such as alpha-tubulin.

16 s including histone H3 (H3), p53, HSP90, and alpha-tubulin.

17 s, shrunken spindles, and hypoacetylation of alpha-tubulin.

18 sed C-terminal glutamates from detyrosinated alpha-tubulin.

19 promote cell migration via deacetylation of alpha-tubulin.

20 XPD, XRCC1, Gli1, Gli2, SHH, IHH, GAPDH and alpha-tubulin.

21 CPP-6, which promotes Delta2 modification of alpha-tubulin.

22 ated by the lack of increased acetylation of alpha-tubulin.

23 activity, resulting in the deacetylation of alpha-tubulin.

24 acts as a K40-specific acetyltransferase for alpha-tubulin.

25 vitro, MEC-17 exclusively acetylates K40 of alpha-tubulin.

26 y increasing HDAC6-mediated deacetylation of alpha-tubulin.

27 t the compound covalently binds to Cys316 of alpha-tubulin.

28 rity during mitosis through interacting with alpha-tubulin.

29 nd deacetylates cytoplasmic proteins such as alpha-tubulin.

30 ronetin perturbs the T7 loop and helix H8 of alpha-tubulin.

31 ater hyperacetylation of the HDAC6 substrate alpha-tubulin.

32 iana tortifolia2 carries a point mutation in alpha-tubulin 4 and shows aberrant cortical microtubule

33 In addition, we used a GFP-tagged alpha-tubulin 6 (GFP-TUA6) to visualise the behaviour of

34 ds of wild-type mice, PACRG colocalizes with alpha-tubulin, a marker for the manchette, whereas this

35 nt coincident with an increase in acetylated alpha-tubulin, a marker of HDAC6 inhibition, by immunohi

36 K drives the dissociation of syndecan-1 from alpha-tubulin, a molecule that may act as an anchor for

37 is hypoacetylated while hyperacetylation of alpha-tubulin, a substrate of histone deacetylase 6 (HDA

38 tified a binder against terminal tyrosine of alpha-tubulin, a unique PTM site.

39 , it also increased the acetylation level of alpha-tubulin, a well-established SIRT2 substrate, in bo

40 Here we found that detyrosinated alpha-tubulin accumulates on correct, more stable, kinet

41 with deacetylase family member 6 (HDAC6) and alpha-tubulin acetyl transferase (alpha-TAT1).

42 Myc-nick induces alpha-tubulin acetylation and altered cell morphology by

43 and its paralog alphaTAT-2 are required for alpha-tubulin acetylation and for two distinct types of

44 on-induced GCN5 degradation, thus recovering alpha-tubulin acetylation and G2/M progression.

45 ocumented the relationship between increased alpha-tubulin acetylation and the aggressive behaviors o

46 his study, we report that elevated levels of alpha-tubulin acetylation are a sufficient cause of meta

47 alpha-Tubulin acetylation at Lys-40, located on the lumi

48 istically, we found that kindlin-2 maintains alpha-tubulin acetylation by inhibiting the microtubule-

49 Independently, we found 35.99% and 16.11% alpha-tubulin acetylation for mouse spinal cord and brai

50 By mimicking alpha-tubulin acetylation genetically, we show both cell

51 738 and ACY-775 induce dramatic increases in alpha-tubulin acetylation in brain and stimulate mouse e

52 t Mec-17 and its paralog are responsible for alpha-tubulin acetylation in Caenorhabditis elegans.

53 g to depletion of ATP and robustly increased alpha-tubulin acetylation in cancer cells.

54 with alpha-tubulin, and it is essential for alpha-tubulin acetylation in EMT.

55 ng novel light on the physiological roles of alpha-tubulin acetylation in mammals.

56 nous SIRT2 expression correlate with reduced alpha-tubulin acetylation in primary mouse cortical neur

57 on and death in patients with high-intensity alpha-tubulin acetylation in primary tumors.

58 ubule dynamics characterized by differential alpha-tubulin acetylation is a hallmark of cancer, neuro

59 t1-null animals display no overt phenotypes, alpha-tubulin acetylation is lost in sperm flagella and

60 Hence, accurate quantitation of alpha-tubulin acetylation is required in human disease a

61 etastatic breast cancer cells exhibited high alpha-tubulin acetylation levels that extended along mic

62 processes, and migrating cells are marked by alpha-tubulin acetylation on lysine 40, a modification t

63 cate that by influencing cellular stiffness, alpha-tubulin acetylation sets the force required for to

64 Reducing alpha-tubulin acetylation significantly inhibited migrat

65 inding of alphaTAT1 onto highly concentrated alpha-tubulin acetylation sites.

66 el antibody-free proteomics assay to measure alpha-tubulin acetylation targeting protease AspN-genera

67 ree proteomics assay enables quantitation of alpha-tubulin acetylation, and is applicable across vari

68 the aggregation-prone mutant SOD1 increased alpha-tubulin acetylation, and the acetylation-mimicking

69 ng of histone deacetylase 6 (HDAC6) increase alpha-tubulin acetylation, endoplasmic reticulum (ER)-mi

70 digit muM level by using the Sirt2 substrate alpha-tubulin-acetylLys40 peptide and inactive up to 100

71 atic activity of the Caenorhabiditis elegans alpha-tubulin acetyltransferase (alpha-TAT) MEC-17 allow

72 We hypothesized that alpha-tubulin acetyltransferase (alphaTAT), which both s

73 alpha-Tubulin acetyltransferase (alphaTAT1) is the major

74 Although it is known that the alpha-tubulin acetyltransferase (alphaTAT1) is the prima

75 There is one such protein known as Atat1 (alpha-tubulin acetyltransferase 1) per higher organism.

76 microtubules, mec-17;atat-2 mutants lacking alpha-tubulin acetyltransferase activity have short micr

77 urse analysis in C.elegans, we show that the alpha-tubulin acetyltransferase ATAT-2 and the signaling

78 Here we demonstrate that mice lacking the alpha-tubulin acetyltransferase Atat1 in sensory neurons

79 Here, we present the structure of the human alpha-tubulin acetyltransferase catalytic domain bound t

80 ysine acetyltransferases of known structure, alpha-tubulin acetyltransferase displays a relatively we

81 We demonstrate here that Atat1 is a major alpha-tubulin acetyltransferase in mice.

82 been limited by the undefined status of the alpha-tubulin acetyltransferase.

83 catalysis, demonstrating that the family of alpha-tubulin acetyltransferases uses a reaction mechani

84 ral and mechanistic information available on alpha-tubulin acetyltransferases.

85 a 'poisoning' mechanism in which R402 mutant alpha-tubulin acts dominantly by populating microtubules

86 explore a potential mechanism of acetylated alpha-tubulin (alpha-Ac-Tub) regulation by Ac-SDKP.

87 ascomycetes likely possessed two paralogs of alpha-tubulin (alpha1/alpha2) and beta-tubulin (beta1/be

88 Ulk4 also regulated acetylation of alpha-tubulin, an important post-translational modificat

89 Using a proteomics approach, we identified alpha tubulin and beta tubulin as proteins that interact

90 turn modify cytoplasmic proteins, including alpha-tubulin and ATG3.

91 rometry, we find that CLIP-170 binds to both alpha-tubulin and beta-tubulin, and that binding is not

92 correctly spliced mRNAs, including those for alpha-tubulin and Bub1, and impairs cell cycle progressi

93 t 12l could upregulate acetylation of H3 and alpha-tubulin and downregulate mTOR-related downstream m

94 Mutation of the acetylation site on alpha-tubulin and enzymatic modulation of this posttrans

95 Aurora A-mediated interaction of Merlin with alpha-tubulin and ezrin suggest a potential role for Mer

96 d the subsequent up-regulation of acetylated alpha-tubulin and FGF-21.

97 ry staining of the cells with antibodies for alpha-tubulin and fibronectin showed no difference betwe

98 sion levels of eight genes (SSU-rDNA, actin, alpha-tubulin and five beta-tubulin sequences) to their

99 altered morphology and decreased acetylated alpha-tubulin and glu-tubulin.

100 tionally crucial C-terminal tail sequence of alpha-tubulin and how this interaction catalyzes the tyr

101 ine residues in cytoplasmic proteins such as alpha-tubulin and HSP90.

102 to detyrosinate the C-terminal EEY region of alpha-tubulin and indicates that it is a candidate for t

103 atural product pironetin is known to bind to alpha-tubulin and is a potent inhibitor of microtubule p

104 utoantigens such as vimentin, collagen V, or alpha-tubulin and it has been postulated that autoantibo

105 at SDF-1 treatment induced IQGAP1 binding to alpha-tubulin and localization to CXCR4-containing endos

106 The level of knockdown of two target genes, alpha-tubulin and mitochondrial RNA polymerase (mtpol),

107 ockout podocytes showed dispersed acetylated alpha-tubulin and rare protrusions.

108 enerated flies expressing fusion proteins of alpha-tubulin and rsEGFP2 highlighting the microtubule c

109 on was demonstrated via increased acetylated alpha-tubulin and SOX9 proteins, the number of primary c

110 lar details of the pironetin binding site on alpha-tubulin and thus offer a promising basis for the r

111 gene editing to tag a cytoskeletal protein (alpha-tubulin) and demonstrate a relationship between ex

112 otubule lattice, increased detyrosination of alpha-tubulin, and altered redox signaling.

113 dundantly required for acetylation of MEC-12 alpha-tubulin, and contribute to the function of touch r

114 co-localized with the cytoskeleton protein, alpha-Tubulin, and depolymerization of alpha-Tubulin led

115 also clearly probe the intrinsic beta-actin, alpha-tubulin, and glyceraldehyde 3-phosphate dehydrogen

116 In addition, ARHGAP21 interacts with alpha-tubulin, and it is essential for alpha-tubulin ace

117 s including histones, transcription factors, alpha-tubulin, and nuclear importers.

118 which, in turn, inhibits the acetylation of alpha-tubulin, and promotes the dynamic assembly of micr

119 GCN5 associated with and acetylated alpha-tubulin, and recovering GCN5 protein levels in p38

120 creases HDAC6 activity, increases acetylated alpha-tubulin, and reduces cell migration.

121 MBS and P-MBS contents were normalized to alpha-tubulin, and ROCK activity was expressed as the ra

122 -42 promoted hyperacetylation of H3, H4, and alpha-tubulin, and up-regulation of p21.

123 Immunofluorescence staining with anti-alpha-tubulin antibodies and cell cycle analysis indicat

124 Here, we show that alpha-tubulin appears to be associated with the PADI6/CP

125 Compounds that bind to alpha-tubulin are less well characterized and unexploite

126 hydrolysis trigger conformational changes in alpha-tubulin around an "anchor point," leading to globa

127 Using detection of tyrosinated alpha-tubulin as a marker for new or growing microtubule

128 ed intracellularly and CD44 colocalized with alpha-tubulin as a result of MSU exposure and ECD-sheddi

129 against Phe-Met-Arg-Phe-NH(2) and acetylated alpha-tubulin as well as intracellular injections of Neu

130 Acetylation of alpha-tubulin at conserved lysine 40 (K40) amino acid re

131 tion (H3K36me3) of histones, also methylates alpha-tubulin at lysine 40, the same lysine that is mark

132 quired to increase the levels of tyrosinated alpha-tubulin at the axon injury site and plays an impor

133 LARG colocalizes with alpha-tubulin at the spindle poles before localizing to

134 with the 3'UTR of a constitutively expressed alpha-Tubulin, Bam became stabilized in spermatocytes.

135 The tagged proteins include alpha tubulin, beta actin, desmoplakin, fibrillarin, nuc

136 Isolated Ribbons contain acetylated alpha-tubulin, beta-tubulin, conserved protein Rib45, >9

137 ut is unique in its active site and putative alpha-tubulin binding site.

138 phosphorylation profile along with defective alpha-tubulin-binding properties.

139 nd interacts with the phosphate group of the alpha-tubulin-bound GTP.

140 functional loop directly associates with the alpha-tubulin-bound guanosine triphosphate (GTP) molecul

141 of the ubiquitin conjugation system include alpha-tubulin (but not beta-tubulin), a dynein subunit (

142 ound to induce elevated levels of acetylated alpha-tubulin, but not histone, consistent with its HDAC

143 nd re-ligation of the C-terminal tyrosine on alpha-tubulin, but the purpose of this tyrosination-dety

144 g the injury-induced increase in tyrosinated alpha-tubulin by knocking down TTL impairs retrograde or

145 ly because p38IP promotes the acetylation of alpha-tubulin by preventing the degradation of GCN5, in

146 A new study shows that phosphorylation of alpha-tubulin by the atypical kinase PHS1 leads to rapid

147 arked is by posttranslational acetylation of alpha-tubulin by tubulin acetyltransferase (TAT).

148 d the LINKIN interactors RUVBL1, RUVBL2, and alpha-tubulin by using SILAC mass spectrometry on human

149 lin lysine 40 (K40) or detyrosination of the alpha-tubulin C-terminal tail.

150 e region containing motifs homologous to the alpha-tubulin C-terminal tail.

151 Cellular alpha-tubulin can bear various carboxy-terminal sequence

152 RARRES1 increases the level of detyrosinated alpha-tubulin consistent with a role as the cognate inhi

153 Here, we show that acetylation of MEC-12 alpha-tubulin constrains protofilament number in C. eleg

154 utes to a binding pocket that interacts with alpha-tubulin contained in the longitudinally adjacent a

155 onfirmed the published results, however, the alpha-Tubulin control blot was not found.

156 Our results show that the alpha-tubulin CTT does not protrude out from the microtu

157 This suggests that the interactions of the alpha-tubulin CTT with the tubulin body contributes to t

158 n through an inhibitory interaction with the alpha-tubulin deacetylase HDAC6.

159 was to investigate the effect of EGF-induced alpha-tubulin deacetylation through activating HDAC6 on

160 at involves EGF-induced HDAC6 activation and alpha-tubulin deacetylation, subsequently affecting corn

161 sure the fraction of [(13)C6]leucine-labeled alpha-tubulin-derived surrogate peptides.

162 mitotic error correction, and the extent of alpha-tubulin detyrosination allows centromeric MCAK to

163 egulates tubulin tyrosine ligase, increasing alpha-tubulin detyrosination and promoting microtentacle

164 ctopic expression of Twist or Snail promotes alpha-tubulin detyrosination and the formation of tubuli

165 This increase was supported by elevated alpha-tubulin detyrosination and vimentin protein levels

166 rrect the mitotic errors caused by excessive alpha-tubulin detyrosination independently of its global

167 -SVBP) activities to constitutively increase alpha-tubulin detyrosination near kinetochores compromis

168 Finally, we show that the levels of alpha-tubulin detyrosination remain significantly elevat

169 for mitotic error correction is regulated by alpha-tubulin detyrosination remains unknown.

170 ase whose activity in vitro is suppressed by alpha-tubulin detyrosination-a posttranslational modific

171 phically confirmed natural product to target alpha-tubulin, displays potent cytotoxic activity agains

172 In Chlamydomonas, GFP-tagged alpha-tubulin enters cilia as an intraflagellar transpor

173 d end binding protein 1 (EB1) and YFP-tagged alpha-tubulin expressed in the same cell revealed that t

174 formation of discontinuous, short acetylated alpha-tubulin fragments, and the decrease of microtubule

175 We show that the mCerulean-alpha-tubulin fusion protein localizes to the cytoskelet

176 Moreover, removal of the intron from the alpha-tubulin gene TUB1 restores genome integrity.

177 esulting in an accumulation of detyrosinated alpha-tubulin (Glu-tubulin), and increases microtentacle

178 d points included p53, c-Jun, histone H2A.x, alpha-tubulin, histone H3, alpha tubulin, mitochondrial

179 opic expression of GCN5 efficiently reversed alpha-tubulin hypoacetylation and G2/M arrest.

180 dy are different from those revealed by anti-alpha-tubulin immunostaining, making these two neuronal

181 cleavage of the carboxy-terminal tyrosine of alpha-tubulin impact microtubule functions during mitosi

182 cellular distribution of soluble tyrosinated alpha-tubulin in Elp1-deficient primary sympathetic and

183 t Ikbkap modulates the acetylation status of alpha-tubulin in oocytes, which may at least in part med

184 ment with MTs and that appearance of labeled alpha-tubulin in the dimer pool may be a consequence of

185 DIP13 partially co-localises with acetylated alpha-tubulin in the insect procyclic stage of the paras

186 acetyltransferase (TAT) acetylates Lys-40 of alpha-tubulin in the microtubule lumen.

187 essing of Glu residues from beta- as well as alpha-tubulin in vitro and in vivo.

188 ELP3, and GCN5, have been shown to acetylate alpha-tubulin in vitro, so an important question is how

189 ha-tubulins of one protofilament lie next to alpha-tubulins in the neighboring protofilaments, or the

190 They also increased the acetylation level of alpha-tubulin, indicating that SIRT2 is likely to be the

191 nation, a post-translational modification of alpha-tubulin, influences X-ROS signalling, contraction

192 AC1), substantially increased acetylation of alpha-tubulin instead of histones in the lung cancer cel

193 t ARHGAP21 affects migration and EMT through alpha-tubulin interaction and acetylation.

194 pitation and mass spectrometry analysis that alpha-tubulin interacts with galectin-8 during mitosis.

195 dinal interfaces, as well as movement of the alpha-tubulin intermediate domain and H7 helix.

196 ylation of the epsilon-amino group of K40 on alpha-tubulin is a conserved PTM on the luminal side of

197 Acetylation of alpha-tubulin is an essential constraint on protofilamen

198 Reversible acetylation of alpha-tubulin is an evolutionarily conserved modificatio

199 Reversible detyrosination of alpha-tubulin is crucial to microtubule dynamics and fun

200 These results suggest that newly synthesized alpha-tubulin is first incorporated into MTs or complexe

201 alpha-Tubulin is specifically acetylated on lysine 40, a

202 Acetylation of K40 in alpha-tubulin is the sole posttranslational modification

203 Acetylation of Lys-40 on alpha-tubulin is unique in that it is located on the lum

204 The tuba1a gene encodes a neural-specific alpha-tubulin isoform whose expression is restricted to

205 effects were accompanied by modifications of alpha-tubulin isoforms in the hippocampus, amygdala, and

206 jection of MAP4343 changes the expression of alpha-tubulin isoforms indicative of increased microtubu

207 n microtubule function, including changes in alpha-tubulin isoforms.

208 We conclude that this cell-specific alpha-tubulin isotype dictates the hallmarks of CEM cili

209 We show that Tuba8, another alpha-tubulin isotype previously associated with cortica

210 nesin-2 OSM-3/KIF17, whereas a cell-specific alpha-tubulin isotype regulates ciliary ultrastructure,

211 Using C. elegans, we show that alpha-tubulin isotype TBA-6 sculpts 18 A- and B-tubule s

212 Mutation of the alpha-tubulin isotype TUBA1A is associated with cortical

213 and cilia, and yet the physiological role of alpha-tubulin K40 acetylation is elusive.

214 Because alpha-tubulin K40 acetylation is largely eliminated by d

215 that it is the central determining factor of alpha-tubulin K40 acetylation levels in vivo.

216 ed proteomics assay reported an induction of alpha-tubulin K40 acetylation upon Trichostatin A stimul

217 of alphaTAT1 is necessary and sufficient for alpha-tubulin K40 acetylation.

218 at we name alphaTAT1, with a highly specific alpha-tubulin K40 acetyltransferase activity and a catal

219 Although genetic ablation of the alpha-tubulin K40 acetyltransferase alphaTat1 in mice di

220 alphaTAT1 is the major and possibly the sole alpha-tubulin K40 acetyltransferase in mammals and nemat

221 acetylases because cells fail to deacetylate alpha-tubulin-K40 and histone H3-K9.

222 sibility of this approach by applying it for alpha-tubulin labeling.

223 acellular peptides but altered the levels of alpha-tubulin lacking two C-terminal amino acids (delta2

224 eton alteration and decreased acetylation of alpha-tubulin lead to remodeling of Cx43 in LMNA cardiom

225 tein, alpha-Tubulin, and depolymerization of alpha-Tubulin led to the intracellular accumulation of C

226 ntify a tight correlation between acetylated alpha-tubulin levels and aggressive metastatic behavior

227 on in cultured neurons reduced detyrosinated alpha-tubulin levels and caused severe differentiation d

228 length Myc diminish, Myc-nick and acetylated alpha-tubulin levels are increased.

229 tofilaments, or the "A" configuration, where alpha-tubulins lie beside beta-tubulins.

230 hich TTL increases the levels of tyrosinated alpha-tubulin locally at the injury site to facilitate t

231 odification, the acetylation of lysine 40 of alpha-tubulin, located in the lumen of microtubules, is

232 fy a critical genetic interval harboring two alpha tubulin loci, and we identify a chemically induced

233 Acetylation of alpha-tubulin Lys40 by tubulin acetyltransferase (TAT) i

234 either enriched in or lacking acetylation of alpha-tubulin lysine 40 (K40) or detyrosination of the a

235 n acetyltransferase (alphaTAT1) is the major alpha-tubulin lysine-40 (K40) acetyltransferase in mamma

236 tion of the epsilon-amino group of Lys-40 of alpha-tubulin marks stable microtubules, although the ca

237 s, Vimentin-based intermediate filaments and alpha-tubulin microtubules in Sertoli and McCoy cells.

238 n, histone H2A.x, alpha-tubulin, histone H3, alpha tubulin, mitochondrial membrane potential, mitocho

239 Specific posttranslational alpha-tubulin modifications are critical for adherent ce

240 b deacetylase that specifically deacetylates alpha-tubulin, modulates microtubule dynamics, and promo

241 the peribiliary network stain positively for alpha-tubulin, mucins, and chromogranin A, as well as fo

242 tylation, and the acetylation-mimicking K40Q alpha-tubulin mutant promoted mutant SOD1 aggregation.

243 ctively reversed by an acetylation-mimicking alpha-tubulin mutant.

244 Tetrahymena MEC-17 gene phenocopies the K40R alpha-tubulin mutation and makes microtubules more labil

245 unction in the conserved MAPKKK dlk-1 and an alpha-tubulin mutation, specifically blocks synapse remo

246 We identified four beta-tubulin and two alpha-tubulin mutations in patients with a spectrum of c

247 tation of the TUBA8 gene, encoding a variant alpha-tubulin of unknown function that is not susceptibl

248 nct arrangements: "B-lattices," in which the alpha-tubulins of one protofilament lie next to alpha-tu

249 was accompanied by increased acetylation of alpha-tubulin on Lys40.

250 Acetylation of alpha-tubulin on lysine 40 marks long-lived microtubules

251 s of either the MEC-7 beta-tubulin or MEC-12 alpha-tubulin or by growth in 1 mM colchicine causes tou

252 tive spindle function resulting from altered alpha-tubulin or class III beta-tubulin overexpression.

253 , Plk1 cKO oocytes either failed to organize alpha-tubulin or developed an abnormally small bipolar s

254 ng enhanced green fluorescent protein-tagged alpha-tubulin or EB1 protein.

255 s of either the MEC-7 beta-tubulin or MEC-12 alpha-tubulin or growth in 1 mM colchicine) cause a gene

256 in the complex B component IFT74/72, but not alpha-tubulin or p28, a component of an inner dynein arm

257 d delta2-tubulin from purified porcine brain alpha-tubulin or polymerized HEK293T microtubules.

258 ), a major posttranslational modification of alpha-tubulin, or whether proteolytic cleavage of the C-

259 f 10-80% mixture of acetylated/nonacetylated alpha-tubulin peptides in the background of human olfact

260 , tissue-selective TTLL-mediated pathway for alpha-tubulin polyglutamylation that is required for spe

261 in conjunction with substantial reduction in alpha-tubulin polyglutamylation, which closely correlate

262 C, it was identified that the Histone H3 and alpha-Tubulin purification control blots for YES and LYN

263 with GCN5 increased, and the acetylation of alpha-tubulin reached a peak.

264 relocalization to promote its activation and alpha-tubulin recruitment during migration.

265 Acetylation of alpha-tubulin residue Lysine-40 (K40) has been correlate

266 vity, as well as by the identification of an alpha-tubulin residue specifically required for the Kip3

267 ively abolished EGF-induced deacetylation of alpha-tubulin, resulting in the inhibition of cell migra

268 -hydroxybenzamide (3f) on the acetylation of alpha-tubulin revealed an increased level of acetylation

269 t measured SIRT2 deacetylation of acetylated alpha-tubulin revealed that propofol inhibits enzymatic

270 molecular dynamics simulations-suggest that alpha-tubulin's amphipathic helix H10 is responsible for

271 ng arginine at position 402 (R402) of TUBA1A alpha-tubulin selectively impair dynein motor activity a

272 In dynamin 1-depleted MPCs by RNAi, alpha-tubulin showed a dispersed linear filament-like lo

273 ctural elements that play important roles in alpha-tubulin-specific acetylation.

274 ificity is achieved by interactions with the alpha-tubulin substrate that extend outside of the modif

275 les became rapidly stabilized and that their alpha-tubulin subunit posttranslationally detyrosinated

276 on of the C-terminal tyrosine and the acidic alpha-tubulin tail by VASH1.

277 inhibiting severing, and that detyrosinated alpha-tubulin tails are the least effective.

278 sequences of alpha-, beta-, or detyrosinated alpha-tubulin tails that have been covalently linked to

279 a provide a basis for the rational design of alpha-tubulin targeting chemotherapeutics.

280 re, we find that three tubulin isotypes--the alpha-tubulins TBA-6 and TBA-9 and the beta-tubulin TBB-

281 We also discovered an alpha-tubulin (TBA-7) that appears to destabilize MTs.

282 rafish expressing a photoconvertible form of alpha-tubulin (tdEOS-tubulin) specifically in cone photo

283 showed strong phosphorylation of endogenous alpha-tubulin that could be blocked when S165N-alpha6-tu

284 een stathmin's N terminus and the surface of alpha-tubulin that is exposed only at the minus end.

285 an enzyme that acetylates the K40 residue of alpha-tubulin, the only PTM known to occur on the lumina

286 This result indicates that the alpha-tubulin tyrosine facilitates initial motor-tubulin

287 lts reveal a strong effect of the C-terminal alpha-tubulin tyrosine on dynein-dynactin motility and s

288 provide preclinical rationale for acetylated alpha-tubulin use as a pharmacodynamic biomarker in futu

289 approximately half of KIF17 and one third of alpha-tubulin utilizes diffusion besides IFT.

290 tin forms a covalent bond to cysteine-316 in alpha-tubulin via a Michael addition reaction.

291 Though HDAC6 deacetylates alpha-tubulin, we find that another HDAC6 substrate cont

292 Levels of acetylated alpha-tubulin were decreased in the ischemic hemisphere

293 s R402C and R402H mutations in budding yeast alpha-tubulin, which exhibit a simplified microtubule cy

294 ional effects on the C-terminal H12 helix of alpha-tubulin, which is a likely molecular mechanism for

295 Of note, acetylation of alpha-tubulin, which maintains microtubule flexibility a

296 alphaAsn258, and alphaLys352 amino groups in alpha-tubulin, which supported the formation of a covale

297 ulates microtubule function by deacetylating alpha-tubulin, which suppresses microtubule dynamics and

298 ring the G2/M transition, the association of alpha-tubulin with GCN5 increased, and the acetylation o

299 f calibrations, we site-specifically labeled alpha-tubulin with silicon rhodamine (SiR) in live mamma

300 nd beta-tubulin and also generated a form of alpha-tubulin with two C-terminal Glu residues removed (