ライフサイエンスコーパス: annexin II

1 ll-cell contacts through an interaction with annexin II.

2 A second, 36 kDa, protein was identified as annexin II.

3 ne was found to abolish S-glutathiolation of annexin II.

4 of these were identical to sequences within annexin II.

5 ssue plasminogen activator binding domain of annexin II.

6 nd C335G was equivalent to that of wild type annexin II.

7 B (gpUL55) (gB) can physically interact with annexin II.

8 e conclusion that HCMV gB can bind host cell annexin II.

9 activities are blocked by antibodies against annexin II.

10 th high affinity to a cell surface receptor, annexin II.

11 vator (t-PA) which we recently identified as annexin II.

12 subunit of PI3-kinase and Na,K-beta binds to annexin II.

13 es through the phospholipid-binding protein, annexin II.

14 fic mRNA, and a 7.9-fold increase in surface annexin II.

15 ls as well as altered expression patterns of annexin II.

16 nd this activity was largely attributable to annexin II.

17 membrane domains via their interactions with annexin II.

18 Annexin II, a co-receptor for tissue plasminogen activat

19 Annexin II, a fibrinolytic receptor, binds plasminogen a

20 n (14 amino acids), directly associates with annexin II, a lipid raft-associated molecule, which is a

21 er coat of HIV-1, we define a novel role for annexin II, a PS-binding moiety, as a cellular cofactor

22 lcium-regulated phospholipid-binding protein annexin II, an interaction that is inhibited by the prot

23 Annexin II and alpha-enolase have been reported to be pl

24 amily of proteins, is the cellular ligand of annexin II and also interacts with the C-terminal region

25 ed a calcium-independent interaction between annexin II and an HCMV envelope component.

26 udies revealed high affinity binding between annexin II and beta(2)GPI.

27 promoted the association of Hsp90alpha with annexin II and increased the expression of annexin II on

28 cross-linking experiments with biotinylated annexin II and intact PSV10 cells identified a 55-kDa ba

29 Functional studies demonstrate that both annexin II and S100A10 regulate trafficking of TrpRS.

30 dentified the plasminogen binding protein as annexin II and the angiostatin binding protein as the al

31 all-trans-retinoic acid on the expression of annexin II and the generation of cell-surface plasmin.

32 ssue plasminogen activator binding domain of annexin II and to determine the mechanism of its modulat

33 We demonstrate that annexins II and VI, which contain KFERQ-like sequences,

34 regulated interaction between Hsp90alpha and annexin II, and raise the possibility that increased exp

35 Hence, our results suggest that annexin II, and, likely, annexin I, may be endogenous su

36 t N-terminal peptides of annexin I (AnI) and annexin II (AnII) was investigated under controlled expe

37 Prior work in our laboratory has identified annexin II (Ann-II) as a co-receptor for Plg and t-PA th

38 noprecipitations using anti-CEACAM1 and anti-annexin II antibodies and second by confocal laser micro

39 -beta(2)GPI than control cells and that anti-annexin II antibodies inhibited the binding of (125)I-be

40 s increase in plasmin was blocked by an anti-annexin II antibody and was induced by transfection of t

41 d this change was partially reversed with an annexin II antibody.

42 Annexin II (ANX II) was identified, and confirmed by mat

43 stinal epithelial and colon cancer cells via Annexin II (ANX-II).

44 at corresponded to monomers and multimers of annexin II (Anxa2).

45 APL blasts overexpress annexin II (ANXII), a receptor for tissue plasminogen ac

46 These studies identify annexin II as a plasminogen binding site on macrophages

47 These data establish annexin II as a regulator of cell surface plasmin genera

48 ssary to trigger the activation and identify annexin II as its probable binding partner-receptor.

49 or cDNA showed a similar binding affinity to annexin II as that observed in PSV10 cells.

50 The expression of annexin II, as detected by a fluorescein-tagged antibody

51 but not cysteine 143 or 156 is required for annexin II association with caveolin and the rapid trans

52 ing protein (M(r) of 37 kD) identified it as annexin II (Ax-II).

53 Recently, we identified annexin II (AXII) as a previously unknown factor produce

54 Annexin II (AXII), a calcium-dependent phospholipid-bind

55 Recombinant annexin II, bearing the calcium-binding site substitutio

56 125I-Labeled annexin II binding assays with PSV10 cells demonstrated

57 Using 125I-labeled annexin II binding to screen NIH3T3 transfected with a h

58 We showed that annexin II binds to normal primary human marrow stromal

59 roximal crypt cells, providing evidence that annexin-II binds PG in situ in colonic crypt cells.

60 Antibodies to annexin II blocked all three cellular responses to TNfnA

61 calcium-binding "endonexin" motif (KGXGT) of annexin II, blocked its interaction with endothelial cel

62 Exogenous 125I-annexin II bound to EGTA-washed endothelial cells with h

63 nexin II was inhibited by intact fluid phase annexin II but not by its "core" fragment (residues 25-3

64 extracellular vesicle (EV) markers, CD63 and annexin II, but attenuated lysosome-multivesicular body

65 f synthesis of the thiol-containing protein, annexin II, but no change in synthesis of the cysteinele

66 6 kDa protein was subsequently identified as annexin II by proteomic approach and confirmed by Wester

67 Moreover, this HIV-1 PS interaction with annexin II can be disrupted by SLPI or other annexin II-

68 ed that the endothelial cell surface protein annexin II can stimulate t-PA-mediated plasminogen activ

69 ophy, including those coding for Sp3, c-Jun, annexin II, cathepsin B, and HB-EGF, thus showing the ge

70 , Ch), G3PDH (Hu, Ch), pyruvate kinase (Ch), Annexin II (Ch), and protein disulfide isomerase (Ch).

71 Ligand blots demonstrated that annexin II comigrates with one of several proteins in ly

72 transfection of t(15;17)-negative cells with annexin II complementary DNA.

73 16 h of biosynthesis, and that cell surface annexin II comprises 4.3 +/- 1.0% of the total cellular

74 The PS-annexin II connection may represent a new target to prev

75 um, caveolin IgG precipitated four proteins: annexin II, cyclophilin 40, caveolin, and cyclophilin A.

76 Capillary sprouting from annexin II-deficient aortic ring explants was markedly r

77 In addition, annexin II-deficient mice displayed markedly diminished

78 nied by an increased amount of extracellular annexin II detected in the media of PC12, NIH-3T3(IR), a

79 od monocytes represent the major circulating annexin II-expressing cell.

80 ents, we indeed observed that restoration of annexin II expression inhibited the migration of the tra

81 y insulin caused a temporary dissociation of annexin II from these receptors, which was accompanied b

82 Immunodepletion of annexin II from type II cell cytosol reduced its fusion

83 Annexin II has been implicated in membrane fusion during

84 Cellular annexin II has been shown to specifically bind human cyt

85 Likewise, anti-annexin II IgG directed against the t-PA-binding tail do

86 of RAW264.7 macrophages with monoclonal anti-annexin II IgG inhibited (35%) their binding of 125I-Lys

87 Preincubation of THP-1 monocytes with anti-annexin II IgG inhibited (60%) their plasminogen-depende

88 Upon treatment with anti-annexin II IgG or alpha(2)AP, conditioned medium from he

89 jor physiological plasmin inhibitor, or anti-annexin II IgG, blocked EMT by approximately 80%, and 50

90 Anti-annexin II IgG-mediated inhibition of EMT was overcome b

91 vely) when cells were preincubated with anti-annexin II IgG.

92 s recovered by immunoprecipitation with anti-annexin II IgG.

93 A role for annexin II in binding of beta(2)GPI to cells was confirm

94 copy showing co-localization of CEACAM1 with annexin II in mammary epithelial cells grown in Matrigel

95 IIt complex at the plasma membrane, and with annexin II in secretory vesicles.

96 crophages and indicate an important role for annexin II in their invasive and degradative phenotype.

97 escribed receptor for fibrinolytic proteins, annexin II, in cells from patients with APL or other leu

98 of the crystal adhesion molecules, CD44 and annexin II, in tubular epithelial cells in vitro and in

99 en 2 (SCCA2), S100A8, S100A9, Annexin I, and Annexin II] in the squamous NHTBE cells was further conf

100 These data suggest that translocated annexin II interacts with cell surface phospholipid via

101 Annexin II is a heterotetramer, consisting of two 11-kDa

102 Here, we demonstrate that annexin II is constitutively translocated to the endothe

103 Annexin II is expressed by most sensory neurons of the D

104 Annexin II is secreted into the extracellular environmen

105 We also show that the PKC substrate, annexin II, is required for FR internalization.

106 ith a highly significant correlation between annexin II levels and factors associated with impeded fi

107 Here we identify annexin II light chain (p11) as a regulatory factor that

108 The annexin II light chain (p11), one of the two subunits of

109 ces the selective loss of p11 (also known as annexin II light chain, S100A10), a multifunctional prot

110 We conclude that a caveolin-annexin II lipid-protein complex facilitates the rapid i

111 Overexpression of annexin II may be a mechanism for the hemorrhagic compli

112 Thus, annexin II-mediated assembly of plasminogen and t-PA on

113 In the present study, we investigated annexin II-mediated membrane fusion by using isolated la

114 Here, we show that annexin II-mediated plasmin activity regulates release o

115 Our results suggest that annexin II-mediated plasmin activity regulates the relea

116 These results demonstrate that annexin II mediates the binding of beta(2)GPI to endothe

117 Directed migration of annexin II-null endothelial cells through fibrin and col

118 Here, homozygous annexin II-null mice displayed deposition of fibrin in t

119 Abnormally high levels of expression of annexin II on APL cells increase the production of plasm

120 pendently blocked the stimulatory effects of annexin II on human osteoclast formation, demonstrating

121 ng that the receptor mediates the effects of annexin II on osteoclast formation.

122 Inhibition of plasminogen binding to annexin II on RAW264.7 macrophages significantly impaire

123 Thus, calcium-regulated expression of annexin II on the endothelial cell surface may play a ce

124 h annexin II and increased the expression of annexin II on the surface of aortic ECs.

125 We demonstrated previously that annexin II on the surface of both cultured monocytoid ce

126 s identified the plasminogen binding protein annexin II on the surface of macrophages and determined

127 ence of annexin V but not in the presence of annexin II or VI.

128 re cholesterol, and increased binding of the annexin II-p11 complex to membranes, consistent with oth

129 binding protein that forms a heterotetramer (annexin II-p11 heterotetramer; A2t) with p11 (S100A10).

130 approaches demonstrated that three proteins, annexin II/p36, stratifin/14-3-3 sigma, and heat shock p

131 n lattices in vitro was also reduced, and an annexin II peptide mimicking sequences necessary for t-P

132 tosis (caveolin-1, dynamin-2, Arp2, NSF, and annexin II), phagocytosis (RhoA, dynamin-2, Rac1, and Ar

133 Annexin II, previously implicated in cholesterol traffic

134 Expressed C9G annexin II protein failed to support tissue plasminogen

135 igens involved in signal transduction [e.g., annexin II, protein kinase C alpha, the G alpha subunits

136 Annexin II, recently described as a high-affinity recept

137 t in 5' untranslated region (5'UTR) of human Annexin II receptor (AXIIR) mRNA, there are two upstream

138 body raised against the putative recombinant annexin II receptor also recognized the same 26-kDa prot

139 Importantly, the annexin II receptor antibody dose-dependently blocked th

140 HEK 293 cells transformed with the cloned annexin II receptor cDNA showed a similar binding affini

141 expression library, we identified a putative annexin II receptor clone, which encoded a novel 26-kDa

142 These data show that annexin II receptors on endothelial cells mediate severa

143 emonstrated that there was a single class of annexin II receptors with a Kd of 5.79 nm and Bmax of 2.

144 Direct physical evidence showed that the annexin II-S100A10 complex, which regulates exocytosis,

145 lin and IGF-1 receptor, was shown to inhibit annexin II secretion along with reduced receptor phospho

146 in kinase C had no effect on insulin-induced annexin II secretion, suggesting a possible direct link

147 direct link between receptor activation and annexin II secretion.

148 articipate in molecular mechanisms mediating annexin II secretion.

149 um-dependent phospholipid-binding ability of annexin II since some annexin II was dissociated from vi

150 h and confirmed by Western blotting using an annexin II-specific antibody.

151 annexin II can be disrupted by SLPI or other annexin II-specific inhibitors.

152 acrophages, there was a 2.4-fold increase in annexin II-specific mRNA, and a 7.9-fold increase in sur

153 Annexin II supported t-PA-dependent generation of cell s

154 displayed an additional 3.8-fold increase in annexin II surface expression compared with resident cel

155 n revealed a single reactive cysteine in the annexin II tail domain.

156 Annexin II tetramer (AII(t)) is a member of the Ca(2+)-

157 ion of two of these receptors in MS lesions, annexin II tetramer (AIIt) and low-density lipoprotein r

158 Purified annexin II tetramer (AIIt) induced the fusion of lamella

159 itric oxide (NO) donors on the activities of annexin II tetramer (AIIt), a member of the Ca2+- depend

160 aken together, our results indicate that the annexin II tetramer can serve as a binding protein for p

161 sin B interacts with p11 as well as with the annexin II tetramer in vitro.

162 more, procathepsin B could interact with the annexin II tetramer in vivo as demonstrated by coimmunop

163 Cathepsin B and the annexin II tetramer were shown by immunofluorescent stai

164 chain (p11), one of the two subunits of the annexin II tetramer, was one of the proteins identified.

165 act as a neural cytokine, signaling through annexin II to activate microglia in settings of disease

166 indicating cholesterol-dependent binding of annexin II to membranes.

167 Calcium-dependent binding of annexin II to RAW264.7 macrophages was shown using flow

168 We found that the binding of annexin II to the HCMV envelope occurs partially through

169 uced transport of the membrane-bound form of annexin II to the outside layer of the cell membrane and

170 cells was confirmed by the observations that annexin II-transfected HEK 293 cells bound approximately

171 However, mechanisms regulating annexin II transport across the cellular membrane are un

172 Upon exposure to homocysteine, annexin II underwent a 135 +/- 4-Da increase in mass loc

173 However, the exact roles of annexin II, V, and VI and the interaction between annexi

174 hese findings indicate that the formation of annexin II, V, and VI Ca(2+) channels in MV together wit

175 urthermore, K-201 inhibited up-regulation of annexin II, V, and VI gene expression in these cells.

176 concentration, followed by up-regulation of annexin II, V, and VI gene expression, and release of an

177 ntration leads to a further up-regulation of annexin II, V, and VI gene expression, the release of an

178 channel blocker, or antibodies specific for annexin II, V, or VI inhibited the increases in cytosoli

179 In this study, we demonstrate that annexin II, V, or VI mediate Ca(2+) influx into phosphat

180 a-2-loaded PS-enriched liposomes mediated by annexin II, V, or VI, but also inhibited Ca(2+) uptake b

181 Annexins II, V, and VI are major components of matrix ve

182 ch contained significantly higher amounts of annexins II, V, and VI as well as APase activity than ve

183 These findings indicate that annexins II, V, and VI form Ca2+ channels in the plasma

184 I, V, and VI gene expression, the release of annexin II-, V-, VI- and alkaline phosphatase-containing

185 I, V, and VI gene expression, and release of annexin II-, V-, VI- and alkaline phosphatase-containing

186 pid-binding ability of annexin II since some annexin II was dissociated from virions with chelating a

187 lization studies revealed that in NHP cells, annexin II was distributed both in the cytosol and under

188 Annexin II was identified using a glutathione S-transfer

189 plasminogen activator binding to immobilized annexin II was inhibited by intact fluid phase annexin I

190 plastic cells in lung tumor tissues, whereas annexin II was predominant at the cell surface.

191 substantial proportion of virion-associated annexin II was resistant to chelation, which suggested a

192 , we used coimmunoprecipitation to show that Annexin-II was bound to insulin and insulin-like growth

193 embryonic endocardial-derived cells express annexin II which promotes plasminogen activation in vitr

194 L cells contained abundant messenger RNA for annexin II, which disappeared through a transcriptional

195 ll substrate phosphorylation, in particular, annexin II, which is strongly phosphorylated by the gag-

196 the possibility that increased expression of annexin II, which promotes the generation of plasmin, is

197 s report, we characterize the interaction of annexin II with HCMV.

198 thods to inhibit the interaction of tPA with annexin II would offer a new and selective approach to i