ライフサイエンスコーパス: anticodon

1 tRNA(Pro14) D-loop or within the tRNA(Lys43) anticodon.

2 divergent tRNA(Gly)UCA with an opal-decoding anticodon.

3 ng G37 to m(1)G37 on the 3' side of the tRNA anticodon.

4 anscribed with the methionine-specifying CAU anticodon.

5 nylyate residue by recognizing the tRNA(His) anticodon.

6 upling of specific amino acids with specific anticodons.

7 s or by non-native tRNAs with exact-matching anticodons.

8 ated mRNAs, whether this might extend to the anticodon-adjacent position 37 was unknown.

9 Contrary to previous observations, the anticodon adopts the same conformation as seen in mature

10 Ribosomal anticodon-amino acid enrichment further reveals that spe

11 fossil, preserving biological evidence that anticodon-amino acid interactions shaped the evolution o

12 ation on how a tRF is distributed across all anticodon/amino acid combinations, provides alignments b

13 us to report on their genomic distributions, anticodon/amino acid properties, alignments, etc. while

14 eature distributions by isotypes, clades and anticodons, among other tRNA properties such as score.

15 Aminoacyl-tRNA synthetases recognize tRNA anticodon and 3' acceptor stem bases.

16 aRSs may have prevented the conflict between anticodon and amino-acid identities of recruited tRNAs.

17 of a tRNA gene, a mutation that changes the anticodon and the loss of the ancestral tRNA gene.

18 pairing mismatches between the peptidyl-tRNA anticodon and the mRNA codon dramatically delay this rat

19 thylation of tRNA(Pro) on the 3' side of the anticodon and the translation factor EF-P.

20 n the first two nucleotides of the codon and anticodon and then is stabilized by base-stacking energy

21 Anticodon loop nucleotide 37 is 3' to the anticodon and, in tRNACGGPro, is methylated at the N1 po

22 EAT-like domain to recognize the appropriate anticodons and position the hypermodified nucleoside int

23 Overexpressed angiogenin (ANG) cleaves tRNA anticodons and produces tRNA halves similar to those pro

24 pecifier sequence, which recognizes the tRNA anticodon, and the antiterminator bulge, which base pair

25 A and tRNA with cognate unnatural codons and anticodons, and after the tRNA is charged with a noncano

26 ural codons and tRNAs with cognate unnatural anticodons, and their efficient decoding at the ribosome

27 aryotes, transfer RNAs (tRNAs) with the same anticodon are encoded by multiple nuclear genes, and lit

28 velopment, the pools of mRNA codons and tRNA anticodons are invariant and highly correlated, revealin

29 We show here that anticodons are selectively enriched near their respectiv

30 ancement of the interaction between the tRNA anticodon arm and the Specifier Loop domain.

31 odon interaction, and the length of the tRNA anticodon arm is then measured by the distal loop-loop p

32 known to be responsible for several of the D/anticodon arm modifications, but methylases catalyzing p

33 y altering the structure and dynamics of the anticodon arm of the aminoacyl-tRNA.

34 0S subunit head domain and in flexing of the anticodon arm of tRNA suggests that they represent gener

35 ic properties of Bacillus subtilis tRNA(Tyr) anticodon arms containing the natural base modifications

36 fer RNA occur at five positions in the D and anticodon arms, and at positions G6 and G7 in the accept

37 nstrate that modifications pre-structure the anticodon as a key prerequisite for efficient and accura

38 heless, His-Phe sites appear enriched in His anticodons, as previous L: -His sites also were.

39 minoacylation kinetics, assists proper codon-anticodon base pairing at the ribosome A-site, and preve

40 initiation complexes with established codon-anticodon base-pairing.

41 Modifications of anticodon bases are of particular importance for ribosom

42 S include acceptor stem elements G72/A73 and anticodon bases G35/G36.

43 estricted the conformational dynamics of the anticodon bases of the modified hmtASL(Met)(CAU).

44 that human METTL2 forms a complex with DALR anticodon binding domain containing 3 (DALRD3) protein t

45 fferences between both types of GlyRS at the anticodon binding domain level are evident, the extent a

46 both its N-terminal catalytic and C-terminal anticodon binding domains and that the catalytic domain

47 RNA, as well as the presence of the adjacent anticodon-binding domain (ACB), influences the Nterm con

48 ytic domain and flexibly attached C-terminal anticodon-binding domain (CTD); and (ii) the catalytic d

49 ts A-C) and the Urzyme supplemented with the anticodon-binding domain (fragments A, C, and D).

50 e results entirely from coupling between the anticodon-binding domain and an insertion into the Rossm

51 5% of the contemporary enzyme, including the anticodon-binding domain and connecting peptide 1, CP1,

52 recruitment of connecting peptide 1 and the anticodon-binding domain during evolutionary development

53 he active site of one monomer pairs with the anticodon-binding domain from the other.

54 also propose that structural features of the anticodon-binding domain in MST1 permit binding of the e

55 ylate tRNA(1Thr), reveals differences in the anticodon-binding domain that probably allow recognition

56 gth TyrRS contains a catalytic domain and an anticodon-binding domain; however, the two halves retain

57 e Urzymes acylate cognate tRNAs even without anticodon-binding domains, in keeping with the possibili

58 ned, whereas the new NLS was evolved from an anticodon-binding hexapeptide motif.

59 By reprogramming the anticodon-binding pocket of Pyrococcus horikoshii ProRS

60 Between the 5' anticodon-binding stem I domain and the 3' amino acid se

61 ligomeric transport complex composed of nine anticodon-binding-domain-like units that give rise to a

62 tRNA isodecoders share the same anticodon but have differences in their body sequence.

63 with the same amino acid but using different anticodons), but rather among tRNA genes within the same

64 However, mutations in the tRNA(His) anticodon caused a drastic loss of in vitro histidylatio

65 and carboxylic acid side-chains, whereas the anticodon codes for a wider range of such properties, bu

66 op likely causes tRNA distortion and affects anticodon-codon interaction, which induces +1 frameshift

67 (6)A(37) participate in the stability of the anticodon-codon interaction.

68 ide interactions that disengage the ribosome anticodon-codon interactions for slippage.

69 teractions are required for stability of the anticodon/codon interaction in the ribosomal A-site.

70 nces translation fidelity by stabilizing the anticodon/codon interaction in the ribosomal decoding si

71 ection begins with the base pairing of codon-anticodon complex between the m-RNA and tRNAs.

72 The structures reveal that certain codon-anticodon contexts and the lack of m(1)G37 destabilize i

73 yl-tRNAs (Af-tRNA(Pro)) with three different anticodons: CUA, AGGG, and CUAG.

74 ), and pseudouridine (Psi(39)) in the tRNA's anticodon domain are critical for ribosomal binding and

75 VARS tRNA binding domain and adjacent to the anticodon domain, and disrupt highly conserved residues.

76 ls the htRNA's amino acid accepting stem and anticodon domains in preparation for their being anneale

77 structure reveals stabilization of the codon-anticodon duplex by the N-terminal tail of eIF1A, change

78 and INS require these same acceptor stem and anticodon elements, respectively, whereas YbaK lacks inh

79 Other tRNA(Sec) species with different anticodons enabled E. coli to synthesize active formate

80 nts with two bits per base, we show that the anticodon encodes the hydrophobicity of each amino acid

81 igh-throughput, cell-based assay to identify anticodon engineered transfer RNAs (ACE-tRNA) which can

82 ique sites on proto-tRNAs (distinct from the anticodons), expansion of the code via proto-tRNA duplic

83 Both cognate and near-cognate tRNA anticodons explore the aminoacyl-tRNA-binding site (A si

84 an 500 interspersed tRNA genes comprising 51 anticodon families of largely unequal copy number.

85 Like HisRS, Thg1 utilizes the GUG anticodon for selective tRNA(His) recognition, and Thg1-

86 y conserved Class I active-site residues are anticodons for corresponding Class II active-site residu

87 that of both cognate and near-cognate codon-anticodon helices.

88 RNAs by monitoring the geometry of the codon-anticodon helix in the decoding center using the univers

89 nucleotide G530 stabilizes the cognate codon-anticodon helix, initiating step-wise 'latching' of the

90 nds with the 2'-hydroxyl groups of the codon-anticodon helix, which are expected to be disrupted with

91 ed to be disrupted with a near-cognate codon-anticodon helix.

92 structurally and functionally mimics a codon-anticodon helix.

93 oacceptor family than to tRNAs with the same anticodon in related species.

94 to probe how 8-oxoG interacts with the tRNA anticodon in the decoding center.

95 between the start codon in the mRNA and the anticodon in tRNA(i).

96 ions found at position 37, 3-adjacent to the anticodon, in tRNAs responsible for ANN codons.

97 codon loop, adjacent to the three-nucleotide anticodon, influence translation fidelity by stabilizing

98 equire an RNA pseudoknot that mimics a codon-anticodon interaction and contains a conformationally dy

99 e effect on arrangement of mRNA at the codon-anticodon interaction area.

100 Visualization of the codon*anticodon interaction by X-ray crystallography revealed

101 ontext of A-form RNA and its effect on codon-anticodon interaction during ribosome binding.

102 attributed directly to loss of the critical anticodon interaction has been proposed to explain the c

103 nges that are dependent on whether the codon-anticodon interaction is cognate or near cognate.

104 ough the mRNA also undergoes movement, codon-anticodon interaction is disrupted in the absence of EF-

105 transition from ancient amino acids without anticodon interaction to newer additions with anticodon

106 is first examined through specifier sequence-anticodon interaction, and the length of the tRNA antico

107 In addition to the specifier-anticodon interaction, two interdigitated T-loops near t

108 nticodon interaction to newer additions with anticodon interaction.

109 o maintain structure and stabilize the codon-anticodon interaction.

110 ough the channel and/or disrupt A-site codon-anticodon interaction.

111 ntral domain pseudoknot that resembles codon-anticodon interactions and prevents PKR activation by VA

112 l decoding centre dynamically monitors codon-anticodon interactions before and after GTP hydrolysis.

113 region, stem II locally reinforces the codon-anticodon interactions between stem I and tRNA, achievin

114 I hypothesise that codon-anticodon interactions of tRNAs with mRNA evolved as a m

115 bind specific tRNA anticodons through codon-anticodon interactions with the nucleotide triplet of th

116 for a complete set of single-mismatch codon-anticodon interactions.

117 Disrupting a conserved base pair in the anticodon-intron helix dramatically reduces tricRNA leve

118 The three-dimensional structure of the anticodon is crucial to tRNA-mRNA specificity, and the d

119 34 (U34) at the wobble position of the tRNA anticodon is post-transcriptionally modified, usually to

120 ancies by grouping Pol III occupancy into 46 anticodon isoacceptor families or 21 amino acid-based is

121 s located in the anticodon loop, outside the anticodon itself, stabilize tRNA-codon interactions, inc

122 s an unusual tRNA(Thr) with an enlarged 8-nt anticodon loop ( ).

123 quence is correlated with a base pair in the anticodon loop (nucleotides 32 and 38) to tune the bindi

124 usual tRNA(1Thr), which contains an enlarged anticodon loop and an anticodon triplet that reassigns t

125 tides that pair with nucleotides in the tRNA anticodon loop and is flanked on one side by a kink-turn

126 anine nucleotide 37 (m(1)G37) located in the anticodon loop andimmediately adjacent to the anticodon

127 nine tRNA isoacceptors are methylated in the anticodon loop by the METTL2 methyltransferase to form t

128 cellular processes, including stress-induced anticodon loop cleavage of mature tRNAs to generate tRNA

129 ngstrom resolution shows a reordering of the anticodon loop consistent with the findings from the hig

130 the tRNA at the 5' end while maintaining the anticodon loop for potential loop-loop interactions.

131 Our data show that MST1 recognizes the anticodon loop in both tRNAs, but employs distinct recog

132 3 is required for deposition of m(5)C at the anticodon loop in the mitochondrially encoded transfer R

133 not result in this signature feature of the anticodon loop in tRNA(Tyr).

134 the hydrogen bond network of the unmodified anticodon loop including a C(32)-A(38)(+) base pair and

135 The size but not the sequence of the anticodon loop is critical for tRNA(1Thr) recognition, w

136 The insertion of U33.5 in the anticodon loop likely causes tRNA distortion and affects

137 wobble position of certain tRNAs, a critical anticodon loop modification linked to DNA damage surviva

138 Anticodon loop nucleotide 37 is 3' to the anticodon and,

139 enosine (t(6)A), found at position 37 in the anticodon loop of a subset of tRNA.

140 against a fungal ribotoxin that incises the anticodon loop of cellular tRNAs.

141 sufficient to promote the U-turn fold of the anticodon loop of Escherichia coli tRNA(Phe), but these

142 elucidated the nucleotide requirement in the anticodon loop of hmtRNAs, and revealed mechanisms invol

143 the contributions of individual bases in the anticodon loop of hmtRNAThr to t6A modification.

144 s site, NEG contacts 16S rRNA as well as the anticodon loop of the A-site tRNA.

145 re shows how SmpB plays the role of both the anticodon loop of tRNA and portions of mRNA to facilitat

146 's in hairpin loops, which is similar to the anticodon loop of tRNA targeted by adenosine deaminases

147 accessibility for pairing with bases in the anticodon loop of tRNA.

148 omain in MST1 permit binding of the enlarged anticodon loop of tRNA1(Thr).

149 Here we show that cytosine 32 in the anticodon loop of Trypanosoma brucei tRNA(Thr) is methyl

150 These enzymes affect the anticodon loop of various tRNAs and can impact protein s

151 vative containing an extra nucleotide in its anticodon loop that undergoes +1 frameshifting, reveal t

152 We identified tRNA(Opt)AUG anticodon loop variants that increase reassignment of th

153 ions can induce cleavage of tRNAs around the anticodon loop via the use of the ribonuclease angiogeni

154 We found that an extended anticodon loop with an extra nucleotide was required for

155 s through pairing of nucleotides in the tRNA anticodon loop with nucleotides in the Specifier Loop do

156 Modifications in the tRNA anticodon loop, adjacent to the three-nucleotide anticod

157 IPTase recognition sequence A36A37A38 in the anticodon loop, only tRNA(Ser)AGA, tRNA(Ser)CGA, tRNA(Se

158 Additionally, modifications located in the anticodon loop, outside the anticodon itself, stabilize

159 an unusual thiolation at position 33 of the anticodon loop, the only known modification at U33 in an

160 es into ap/P and pe/E states, in which their anticodon loops are bound between the 30S body domain an

161 recently was demonstrated to hypermodify the anticodon loops in some tRNAs.

162 aminoacylates two natural tRNAs that contain anticodon loops of different size and sequence.

163 RNAs, tRNA1(Thr) and tRNA2(Thr), that harbor anticodon loops of different size and sequence.

164 ng post-transcriptional modifications of the anticodon loops of four tRNAs in Escherichia coli.

165 ngle aaRS can recognize completely divergent anticodon loops of natural isoacceptor tRNAs and that in

166 recently showed that ANG cleaves tRNA within anticodon loops to produce 5'- and 3'-fragments known as

167 ivated ribonuclease that cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced f

168 hanism by which a single aaRS binds distinct anticodon loops with high specificity is not well unders

169 t probably allow recognition of the distinct anticodon loops.

170 ibosome selects aminoacyl-transfer RNAs with anticodons matching the messenger RNA codon present in t

171 ilization of the closed PIN state with a UUG-anticodon mismatch.

172 odons has evolved beyond a simple tripeptide anticodon model.

173 and show by direct measurement, that a tRNA anticodon modification from guanosine to queuosine has c

174 rucial for Elongator to maintain proper tRNA anticodon modification levels in vivo.

175 The importance of individual and combined anticodon modifications to the tRNA/HIV-1 Loop I RNA's i

176 s an abstraction of how mRNA codons and tRNA anticodons molecularly interact during protein synthesis

177 site in a manner resembling that of the tRNA anticodon-mRNA codon.

178 a regular tRNA(2Thr) with a threonine (Thr) anticodon, MST1 also recognizes an unusual tRNA(1Thr), w

179 search in hundreds of genomes revealed that anticodon mutations occur throughout the tree of life.

180 Suppressor tRNAs bear anticodon mutations that allow them to decode premature

181 l tRNAs in thrips include gene duplications, anticodon mutations, loss of secondary structures and hi

182 RNA damage inflicted by the Escherichia coli anticodon nuclease PrrC (EcoPrrC) underlies an antiviral

183 fferences in their aminoacyl attachments and anticodon nucleotide sequences.

184 nticodon loop andimmediately adjacent to the anticodon nucleotides 34, 35, 36.

185 Modification of anticodon nucleotides allows tRNAs to decode multiple co

186 We demonstrate that the anticodon nucleotides of these misacylated tRNAs play a

187 vealed conserved residues that interact with anticodon nucleotides.

188 59) contain a G at the first position of the anticodon (numbered 34 of tRNA).

189 ifier trinucleotide that base pairs with the anticodon of cognate tRNA.

190 report the existence of pseudouridine in the anticodon of Escherichia coli tyrosine transfer RNAs (tR

191 ntly create a binding groove specific to the anticodon of its cognate tRNA.

192 ader is inspected for complementarity to the anticodon of methionyl initiator transfer RNA (Met-tRNAi

193 sed on a strategic mutation that changed the anticodon of other tRNA genes to match that of the delet

194 n is the formation of base pairs between the anticodon of the aminoacyl-tRNA and the mRNA codon in th

195 Pairing of the Specifier Sequence with the anticodon of the cognate tRNA is the primary determinant

196 potential base-pairing interactions with the anticodon of the initiator methionyl tRNA.

197 ctions between the codon on the mRNA and the anticodon of the initiator tRNA.

198 Stem I recognizes the overall shape and anticodon of tRNA, while a 3' domain evaluates its amino

199 led that the modification of cytidine in the anticodon of tRNA2(Ile) adds 112 mass units to its molec

200 In most cases, the trinucleotide anticodons of tRNA are important identity determinants f

201 RNA pool, demonstrating that mutation in the anticodons of tRNA genes is a common adaptive mechanism

202 let interactions between codons on mRNAs and anticodons of tRNAs.

203 nal phenotypes, with enzymes known to modify anticodons, or non-tRNA substrates such as rRNA, exhibit

204 lized in the context of a near-cognate codon-anticodon pair.

205 (off) to closed (on)-that occurs upon codon-anticodon pairing in the A site.

206 tension on the mRNA that destabilizes codon-anticodon pairing in the P site and promotes slippage of

207 This interaction mimics codon-anticodon pairing in translation but occurs in the absen

208 racting directly with the mRNA through codon/anticodon pairing.

209 t S12 is involved in the inspection of codon-anticodon pairings in the ribosomal A site, as well as i

210 that a variety of different unnatural codon-anticodon pairs can efficiently mediate the synthesis of

211 To determine the codon-anticodon pairs that are efficiently accepted by the euk

212 tRNA isodecoder gene expression, the overall anticodon pool of each tRNA family is similar across tis

213 opment is controlled in order to generate an anticodon pool that closely corresponds to messenger RNA

214 These findings suggest that while anticodon pools appear to be buffered via an unknown mec

215 of adenosine (A) to inosine (I) at the first anticodon position in tRNA is catalyzed by adenosine dea

216 Modification of the cytidine in the first anticodon position of the AUA decoding tRNA(Ile) (tRNA2(

217 eranylated residues are located in the first anticodon position of tRNAs specific for lysine, glutami

218 red pseudohyphal growth, showing altered CUG anticodon presentation cannot itself induce pseudohyphal

219 ctures explain how tRNA(Ala) is selected via anticodon reading during recruitment to the A-site and u

220 We identified a hexapeptide motif in the anticodon recognition domain that is critical for nuclea

221 the N-terminal half) and only one functional anticodon recognition site (contributed by the C-termina

222 lity that acceptor stem recognition preceded anticodon recognition.

223 rse modifications of nucleotide bases in the anticodon region modulate this specificity.

224 ppreciably stabilized the interaction of the anticodon region with the viral subtype G and B RNAs.

225 ntain a similar overall interaction with the anticodon region, arguing against the sufficiency of thi

226 ng tRNA-derived small RNAs (tDRs) around the anticodon regions.

227 r24)) for cleavage at, or adjacent to, their anticodons, resulting in the generation of tRNA halves.

228 In the tRNA anticodon stem-loop, the anticodon sequence is correlated with a base pair in the

229 ical for tRNA(1Thr) recognition, whereas the anticodon sequence is essential for aminoacylation of tR

230 odons, depending on the identity element and anticodon sequence of the tRNA.

231 e same isodecoder set (tRNAs having the same anticodon sequence).

232 e bacterial ortholog that 'reads' the entire anticodon sequence, MST1 recognizes bases in the second

233 position and the nucleotide upstream of the anticodon sequence.

234 esults demonstrate that disrupting 32-38 and anticodon sequences alters interactions with the ribosom

235 specifically matching amino acids to defined anticodon sequences in tRNAs, ARSs are essential to the

236 amino acid to a set of tRNAs with conserved anticodon sequences.

237 rall architecture of tRNA in addition to its anticodon, something accomplished by large ribonucleopro

238 quence nucleotides, which pair with the tRNA anticodon, stack with their Watson-Crick edges rotated t

239 ed the structure and function of this tRNA's anticodon stem and loop (ASL) domain with these modifica

240 The anticodon stem and loop (ASL) domains of tRNA(Arg1) and

241 the Saccharomyces cerevisiae tRNA(Ile)(IAU) anticodon stem and loop domain (ASL) negates wobble deco

242 nding and functional remodeling of the human anticodon stem and loop domain (hASL(Lys3)) were studied

243 The structure of the tRNA anticodon stem and loop domain (hmtASL(Met)(CAU)), deter

244 The anticodon stem and loop of these isoacceptors (ASL(Arg1,

245 having base changes in the acceptor stem or anticodon stem and loop still retained the ability to co

246 ceptor stem, the first two base pairs of the anticodon stem and the first nucleotide of the variable

247 that modification of the G:C content of the anticodon stem and therefore reducing the structural fle

248 C terminus is required to protect a pre-tRNA anticodon stem from chemical modification.

249 rculosis tRNAs at a single site within their anticodon stem loop (ASL) to generate tRNA halves.

250 he 30S ribosomal subunit in complex with the anticodon stem loop of tRNA(Ser) bound to the PsiAG stop

251 s Our structure of QueG bound to a tRNA(Tyr) anticodon stem loop shows how this enzyme uses a HEAT-li

252 position 37 (m(1)G37) modification-deficient anticodon stem loop(Pro), both of which cause the riboso

253 s occupy the 50S subunit E site, while their anticodon stem loops move with the head of the 30S subun

254 base pair in a chimeric tRNA containing the anticodon stem of hmtRNASer(AGY), suggesting that sequen

255 ree consecutive G-C base (3G-C) pairs in the anticodon stem of initiator tRNA.

256 IN state) and by their interactions with the anticodon stem of Met-tRNAi.

257 ed, defining positions in the tRNA(Gln)(CUG) anticodon stem that restrict first base wobble.

258 conserved U32.A38 nucleotide pairing in the anticodon stem through insertion of G37.5.

259 t conserved bases throughout tRNAi, from the anticodon stem to acceptor stem, play key roles in ensur

260 uting the conserved G31:C39 base pair in the anticodon stem with different pairs reduces accuracy (th

261 erved three consecutive GC base pairs in its anticodon stem, play a crucial role in ribosome maturati

262 The anticodon stem-loop (ASL) of transfer RNAs (tRNAs) drive

263 ridine (Psi) formation at position 28 in the anticodon stem-loop (ASL).

264 -tRNA) and in the presence of a near-cognate anticodon stem-loop (ASL).

265 hat streptomycin stabilizes the near-cognate anticodon stem-loop analogue complex, while destabilizin

266 gue complex, while destabilizing the cognate anticodon stem-loop analogue complex.

267 d yet improves recognition of a near-cognate anticodon stem-loop analogue.

268 it in complexes with cognate or near-cognate anticodon stem-loop analogues and messenger RNA.

269 ptomycin disrupts the recognition of cognate anticodon stem-loop analogues and yet improves recogniti

270 Through randomizing bases in anticodon stem-loop followed by a functional selection,

271 The anticodon stem-loop of the A-site tRNA is captured in tr

272 The anticodon stem-loop of tRNAs requires extensive posttran

273 resolution) or two (4.0 angstrom resolution) anticodon stem-loop tRNA mimics bound, that reveal inter

274 In the tRNA anticodon stem-loop, the anticodon sequence is correlate

275 it strategy that completely unfolds the tRNA anticodon stem-loop, which is likely critical for recogn

276 .4-A resolution) of MjPSTK complexed with an anticodon-stem/loop truncated tRNA(Sec) (Mj*tRNA(Sec)),

277 sting formation of correctly folded pre-tRNA anticodon stems in vivo.

278 secutive G-C base pairs (3GC pairs) in their anticodon stems.

279 and a widened angle between the acceptor and anticodon stems.

280 nd differentiation-induced tRNAs often carry anticodons that correspond to the codons enriched among

281 elements in C. crescentus tRNA(His) are the anticodon, the discriminator base and U72, which are rec

282 ity riboswitches that can bind specific tRNA anticodons through codon-anticodon interactions with the

283 stacking while ms(2)t(6)A(37) restrained the anticodon to adopt an open loop conformation that is req

284 vidence that modifications help preorder the anticodon to allow it to recognize the codons, however,

285 quence of the glyQS leader RNA and tRNA(Gly) anticodon to test the effect of all possible pairing com

286 brucei by specifically editing the tRNA(Trp) anticodon to UCA, which can now decode the predominant m

287 t1) modify tRNA position 37, adjacent to the anticodon, to N6-isopentenyladenosine (i6A37) in all cel

288 pid coding systems matching amino acids with anticodon trinucleotides.

289 h contains an enlarged anticodon loop and an anticodon triplet that reassigns the CUN codons from leu

290 ed orthogonal M. jannaschii tRNA with an AUG anticodon: tRNA(Opt) We suspected a modification of the

291 base at the 3' position adjacent to the tRNA anticodon, using S-adenosyl methionine (AdoMet) as the m

292 through cleavage at a single site within its anticodon (UU U).

293 hProRSs show specificity toward a particular anticodon variant of Af-tRNA(Pro), whereas others are pr

294 suspected a modification of the tRNA(Opt)AUG anticodon was responsible for the anomalous lack of codo

295 served nucleotide(A37), adjacent (3') to the anticodon, which is important for the fidelity of codon

296 e substitution at the middle position of the anticodon, which resulted in the change of not only the

297 nally modified at the wobble position of the anticodon with a lysine-containing cytidine derivative c

298 of 5-taurinomethyluridine (taum(5)U) in the anticodon wobble position of five mitochondrial tRNAs.

299 pend on the inosine tRNA modification in the anticodon wobble position.

300 ncm(5) modifications to uridine in the tRNA anticodon 'wobble' position in both yeast and higher euk