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1 en HIV-1 RT and a broad-spectrum UCAA-family aptamer.
2 affinity within a factor of 2 of the parent aptamer.
3 e (L-Tym), interacting with an L-Tym-binding aptamer.
4 reviously described malachite green (MG) RNA aptamer.
5 on-Watson-Crick base-paired region of an RNA aptamer.
6 ye molecule impact its interactions with the aptamer.
7 iption of either the DFHBI aptamer or the MG aptamer.
8 e synthesis of a fluorescence-activating RNA aptamer.
9 inine-bound forms of the cocaine-binding DNA aptamer.
10 s well, but with the antibody replaced by an aptamer.
11 (K(d) = 0.4 nM) than the unmodified original aptamer.
12 target-binding affinities of protein-binding aptamers.
13 s, given the availability of target-specific aptamers.
14 ed of regularly arranged standing-up hairpin aptamers.
15 l conducting polymer nanotubes modified with aptamers.
16 immobilization of amino terminated affinity aptamers.
17 e generated, each consisting of two distinct aptamers.
18 olypyrrole nanotubes (CPNTs) and DA-specific aptamers.
19 s allow single mRNA imaging with fluorogenic aptamers.
20 affinity (30 nM) compared to the full-length aptamer (124 nM), with a limit of detection (LOD) of 2 n
22 ing affinity, and thermal stability make TNA aptamers a powerful system for the development of diagno
24 We screened 13 mutants derived from this aptamer against all these analogues and identified two n
26 very and characterization of the first l-DNA aptamers against a structured RNA molecule, precursor mi
27 Xponential Enrichment (SELEX), we identified aptamers against DUX4 with specific secondary structural
28 chnique (BLI-SELEX) for fishing out specific aptamers against E. coli Shiga toxin subtypes viz., stx1
29 are distinct from previously reported l-RNA aptamers against pre-miR-155, indicating that l-DNA and
30 a one-step technique for rapidly generating aptamers against protein biomarkers in a microtiter plat
32 mple weighted ranking to order the candidate aptamers, all driven within the same GUI-enabled environ
34 constraints on the solution structure of the aptamer and enable computational modeling of the docked
36 uantitatively detects an analyte by using an aptamer and peroxidase mimetic gold nanoparticles that e
38 pt the hybridization equilibrium between the aptamer and the labeled-complementary oligo producing a
39 sub-second resolution via the integration of aptamers and antibodies into a bead-based fluorescence s
40 uantify the occupancy of single Spinach2 RNA aptamers and capture active transcription on single free
41 he present study has investigated the use of aptamers and nanozymes together for the first time in de
42 view will focus on the novel applications of aptamers and SELEX, as well as opportunities to develop
43 nsitively evaluating the binding between DNA aptamers and small-molecule ligands in a high-throughput
44 xploit the molecular recognition capacity of aptamers and the high affinity of aptamers with analyte
45 mphasis on in vitro-selected fluorogenic RNA aptamers and their different modes of ligand binding and
47 sfully functionalized with Abeta-40-specific aptamers and used to detect ultra-low concentrations of
48 , small molecules, peptides and nucleic acid aptamers and we portray work performed with viruses, cel
49 lytic hairpin assembled (CHA) probes, AS1411 aptamer, and pendent biotinylated DNA strand in differen
50 finity and selectivity of the uranyl-binding aptamer, and the distinctive sensing methodology gives r
51 des (nt) from the primer regions of an 80-nt aptamer, and the resulting 61-nt aptamer enhanced bindin
52 on specificity by a broad-spectrum antiviral aptamer, and they open new possibilities for acceleratin
53 or pathogen detection, including antibodies, aptamers, and imprinted polymers, are discussed in terms
54 iple analytes by designing different analyte aptamers, and we further demonstrate that the constructe
59 rature, incubation time and concentration of aptamers) are interdependent, so their dependent study c
60 ription in vitro, we used the "Broccoli" RNA aptamer as a direct, real-time fluorescent transcript re
62 h by reversibly caging a 2'-O-methylated RNA aptamer as well as synthetic threose nucleic acid (TNA)
66 origami nanostructures, functionalized with aptamers, as a vehicle for delivering the antibacterial
67 of the ligand-binding profiles of individual aptamers, as well as the identification of the best targ
68 device for AB diagnosis utilizing a new dual aptamer assay was developed for point-of-care (POC) appl
71 monitor target binding in an electrochemical-aptamer based (EAB) sensor, we achieve subsecond tempora
73 ip between apo M and outcomes using modified aptamer-based apo M measurements among 2170 adults in th
75 We used a large-scale, high-throughput DNA aptamer-based discovery proteomic platform to identify c
76 hitecture, portability, and sensitivity, the aptamer-based FET biosensor has potential as a point-of-
80 proteomic response to infection utilizing an aptamer-based multiplexed proteomics approach to identif
81 he serum protein changes identified with the aptamer-based multiplexed proteomics approach used in th
83 ctive LN patients for 1129 proteins using an aptamer-based platform, followed by ELISA validation in
85 Using a novel protocol we generated 1,132 aptamer-based protein measurements from anti-dsDNA(pos)
91 se findings, we have adapted an impedimetric aptamer-based sensor to the dual recognition of PSA.
92 wo aptamers directly into an electrochemical aptamer-based sensor, which achieved a detection limit o
96 anti-interferon-gamma (IFN-gamma) molecular aptamer beacon (MAB) attached to a bioluminescent protei
100 cells in culture, as the selectivity of the aptamer binding directs doxorubicin into the aptamer-tar
101 90-231) phase separated into large droplets, aptamer binding increased the number of droplets but not
102 r domain is effective as a fusion to the MS2 aptamer binding protein MCP, allowing the construction o
103 t protein and subsequently recruited by gRNA aptamer binding to a nuclease competent CRISPR complex c
105 applicable method for generating near-ideal aptamer biosensors for various analytical applications,
107 vivo effects of a third-generation anti-VWF aptamer (BT200) before/after stimulated VWF release.
108 on rate of the complementary strand with the aptamer but does not impact its dissociation rate, sugge
110 strategy to improve the binding affinity of aptamers by modifying their sequences upon their G-quadr
111 aptamers, we also chemically modified these aptamers by substituting their 2'-OH group with 2'-fluor
112 ingly, our data indicated that one candidate aptamer, called V15, can specifically inhibit the in vit
113 These findings reveal that a riboswitch aptamer can function independently of any overlapping ex
116 n of the best target binders from a batch of aptamer candidates, independent of the ligands in questi
118 n this work, we have rationally truncated an aptamer capable of recognizing gliadin in a deep eutecti
119 ancers, with antibodies, proteins, peptides, aptamers, carbohydrates and small molecules all exploite
121 ular recognition tool for many applications, aptamers complement the use of antibodies due to many un
122 RNA-binding properties can be achieved using aptamers composed of l-DNA, which has several practical
123 ht the recent advances in the development of aptamer-conjugated nanomaterials and their utilization f
125 a central cavity with a target-specific DNA aptamer coupled with a nanopore read-out to enable indiv
128 our new finding that ligand binding inhibits aptamer digestion by T5 exonuclease, where the extent of
131 sed to target the guanine-sensing riboswitch aptamer domain (GSR(apt)) of the xpt-pbuX operon in Baci
132 uanidine-induced pseudoknot formation by the aptamer domain of a guanidine III riboswitch from Legion
133 We present the crystal structure of the aptamer domain of this atypical cobalamin riboswitch and
134 ion of alternative molecules (e.g. proteins, aptamers) during the design process, and the export opti
139 of an 80-nt aptamer, and the resulting 61-nt aptamer enhanced binding affinity by 19 times (K(d) = 1.
142 of magnitude compared to the linear peptide aptamer, estimating K(D) as 10.1 nM, which is the lowest
143 rty in two parts of a nanoassembly: 1) in an aptamer evolved from a six-letter DNA library to selecti
147 eted exosomal delivery systems engineered by aptamer for future strategies to promote human health us
149 ribe the selection and identification of DNA aptamers for bacterial cells using a combined approach b
150 oach, we investigated two well-characterized aptamers for cocaine/quinine (MN4), chosen for its nanom
153 Here, we report a novel array of Mango II aptamers for RNA imaging in live and fixed cells with hi
155 is generally difficult to identify the best aptamer from the resulting sequences, and the selected a
157 The data show that GNPs with or without aptamer functionalization could form a nanoparticle-asse
158 electrodes, with the specificity provided by aptamer functionalization of the microelectrode surface.
161 logy, we also present the stimuli-responsive aptamer-functionalized MOFs for sensing, followed by a b
162 , this article reviews recent innovations of aptamer-functionalized MOFs-based biosensors and their b
164 in PCR-based diagnostics, high-affinity DNA aptamer generation, site-specific labeling of RNAs, semi
165 with receptor and catalytic characteristics, aptamers have been standing out, owing to their target-i
166 on nanoparticles (UCNPs) functionalized with aptamers have been suggested as easy-to-use platforms, t
167 al as well as early phase human studies with aptamers have not shown safety concerns to date and have
170 tasensor was developed based on aflatoxin B1 aptamer immobilized on Carbon quantum dots/octahedral Cu
171 e its hybridization kinetics with individual aptamers immobilized on a surface and located with super
174 study reports a novel sensing strategy using aptamer-induced fluorescence fluctuation of graphene qua
180 These results illuminate features at the RT-aptamer interface that govern recognition specificity by
181 t a methodology for converting virtually any aptamer into a molecular switch with pH-selective bindin
182 of two F30-scaffolded dimers of the Broccoli aptamer into a SINV cDNA clone using sites in nsP3 (geno
183 envelope protein domain III (ED3)-targeting aptamers into a two-dimensional pattern precisely matchi
192 ghput sequencing can enhance the analysis of aptamer libraries generated by the Systematic Evolution
194 demonstrate a successful application of the aptamer modification strategy and the associated aptamer
196 diluted urine samples using a uranyl-binding aptamer-modified silicon nanowire-based field-effect tra
198 ucted by sequential conjugation of CPNTs and aptamer molecules on the IMEs, and the substrate was int
199 cal approaches for the identification of RNA aptamer motifs and uses a simple weighted ranking to ord
203 om the resulting sequences, and the selected aptamers often exhibit suboptimal affinity and specifici
204 rates two identical biotin-labeled truncated aptamers, one of which is immobilized on a carbon screen
207 ith a new class of affinity ligands known as aptamers or chemical antibodies, molecularly targeted ex
209 zyme, N,N',N"-triacetylchitotriose (TriNAG), aptamer, p-aminobenzamidine (pABA), bovine pancreatic ri
210 Post-SELEX aptamer engineering can improve aptamer performance, but current methods exhibit inheren
211 control protocol for the characterization of aptamer performances coupled with the observation of ind
213 fluorescence confocal microscopy, ELISA, and aptamer proteomics were used to identify and validate LA
214 e to l-RNA for the generation of RNA-binding aptamers, providing a robust and practical approach for
216 ally, we conjugated one of the aptamer RCAs (Aptamer RCA II) to M2e epitope peptide of influenza viru
220 er candidates, 4 aptamer-based RCA products (aptamer RCAs) were generated, each consisting of two dis
221 N leads to the transcription of the broccoli aptamer recognizing the DFHBI ligand and of the aptamer
222 amer recognizing the DFHBI ligand and of the aptamer recognizing the malachite green (MG) ligand, the
223 the red fluorescent variant of the Broccoli aptamer, Red Broccoli, does not exhibit red fluorescence
224 elieve our approach is generalizable for DNA aptamers regardless of sequence, structure, and length a
227 Multiparametric structural analysis of these aptamers revealed that A1 adopts a hairpin conformation.
230 y to rapidly generate metabolite-binding RNA aptamers, RNA-based sensors have the potential to be des
233 and MLANA and novel biomarkers validated by aptamer screening, ELISA, and immunofluorescence microsc
237 d the recent development and applications of aptamer sensors (aptasensors) based-on nanomaterial for
239 platform for screening of the minimal-length aptamer sequence required for high-affinity target bindi
241 was finely tuned by rational engineering of aptamer sequences to define receptor motion and/or nativ
243 we could verify that none of the ampicillin aptamers show any specific binding with their intended t
245 SAR and hydroxyl radical probing identified aptamer structural elements critical for inhibition and
246 nally, comparison with published protein-RNA aptamer structures pointed toward more general features
248 ecent progress is discussed in incorporating aptamer switches into more complex synthetic nucleic aci
253 omic viral RNAs tagged with the Broccoli RNA aptamer that binds and activates a conditional fluoropho
255 results reveal a red fluorescent fluorogenic aptamer that functions in mammalian cells and that can b
257 Spinach and Broccoli are fluorogenic RNA aptamers that bind DFHBI, a mimic of the chromophore in
261 se permitted the isolation of functional TNA aptamers that bind to HIV reverse transcriptase (HIV RT)
263 complex with A9g, a 43-bp PSMA-specific RNA aptamer, that was determined to the 2.2 angstrom resolut
265 sed to a S-adenosyl methionine (SAM)-binding aptamer to generate a red fluorescent RNA-based sensor t
267 ughput engineering of small-molecule-binding aptamers to acquire those with improved binding properti
268 es enable single mRNA containing 24 Broccoli aptamers to be imaged in live mammalian cells treated wi
269 pared the RCA products that consist of these aptamers to increase the spanning space and overall bind
270 -strand recovery step in our SELEX to direct aptamers to the surface of erythrocytes infected with P.
271 size that a conformational change in the DNA aptamer upon specific binding of HA protein may alter th
272 These sensing platforms rely on selecting aptamers using systematic evolution of ligands by expone
275 ingle-stranded deoxyribonucleic acid (ssDNA) aptamer was specially designed and synthesized to detect
280 ria, and quantum dots (QD) bound to a second aptamer were utilized to quantify the amount of bacteria
282 ions; magnetic beads coated with AB-specific aptamers were used to capture bacteria, and quantum dots
284 enhance the binding characteristics of a DNA aptamer which binds indiscriminately to ATP, ADP, AMP, a
285 wed by bio-recognition using a specific CD63 aptamer, which was conjugated to horseradish peroxidase
286 erent functionalization methods of MOFs with aptamers, which provide a foundation for the constructio
287 ucleic acid nanostructures, and nucleic acid aptamers, which, respectively, provide the ability to en
289 the rapid and efficient identification of an aptamer with both high affinity and high specificity.
293 apacity of aptamers and the high affinity of aptamers with analyte to trigger TiO(2)@AgNP substrates
294 d Fusobacterium nucleatum glycine riboswitch aptamers with and without glycine, Mycobacterium SAM-IV
295 , we report the use of a set of modified DNA aptamers with enhanced chemical diversity to probe the c
296 remarkable property, we use SFM4-3 to select aptamers with large hydrophobic 2' substituents that bin
298 demonstrate the design of tightly regulated aptamers with strong target affinity over only a narrow
300 gin our analysis with small-molecule binding aptamers, with emphasis on in vitro-selected fluorogenic