ライフサイエンスコーパス: apyrase

1 rbenoxolone) or degrading extracellular ATP (apyrase).

2 NOate, or increased ectonucleotidase levels (apyrase).

3 tasis in contrast to nontarg-CD39 and potato apyrase.

4 nstructed constitutively expressing the GS52 apyrase.

5 y preincubation with oxidized ATP, KN62, and apyrase.

6 ase activity or proper folding of this human apyrase.

7 ntrations of ADP, in contrast to R. prolixus apyrase.

8 EGTA or by removal of extracellular ATP with apyrase.

9 recently discovered mammalian lysosomal endo-apyrase.

10 B6 and CD39L3) is a membrane-associated ecto-apyrase.

11 cto-apyrase and the tunicamycin-treated ecto-apyrase.

12 codes the first reported human secreted ecto-apyrase.

13 ct was rescued by intratracheal injection of apyrase.

14 tches the value found for Lutzomyia salivary apyrase.

15 e hydrolase of Toxoplasma gondii is a potent apyrase.

16 t in the glandular cells containing the ecto-apyrase.

17 mune cells suggests that CD39 may be an ecto-apyrase.

18 eversed by removing ATP by centrifugation or apyrase.

19 micro M), close to the values obtained with apyrase.

20 ation, or the presence of the ADP scavenger, apyrase.

21 TXA2 receptor (SQ29548), or by intratracheal apyrase.

22 nt of P2X7 and unaffected by incubation with apyrase.

23 2) receptor knockdown or ATP hydrolysis with apyrase.

24 ysteine, and reduced by the exogenous ATPase apyrase.

25 of the EGFR near wounds is not sensitive to apyrase.

26 ogated by eliminating ATP in the medium with apyrase.

27 stronger extent then nontarg-CD39 and potato apyrase.

28 evelopment appeared normal in the absence of apyrases.

29 , suggesting that they might encode secreted apyrases.

30 antiaggregatory effects of the administered apyrases.

31 Apyrase (10 units/ml), which rapidly hydrolyzes 5' nucle

32 tosolic adenosine triphosphate (ATP) (3 U/mL apyrase; -12.58 +/- 1.45 pA/pF), and by the putative PKC

33 ith a high concentration of the nucleotidase apyrase (17 +/- 5 pA/pF for 10 IU/ml and 11 +/- 3 pA/pF

34 ical perfusion of the ATP diphosphohydrolase apyrase (2 U ml(-1) ), or the ATPase inhibitor ARL67156

35 Such activity was blunted by apyrase, 2'-deoxy-N6-methyladenosine 3',5'-bisphosphate

36 We found that the CD39 mimic apyrase (250 U/kg) decreased and knockout or pharmacolog

37 hibited in the presence of the ATP hydrolase apyrase (3 units/ml) or by exposure to the P2 receptor b

38 ,-4'-disulfonic acid (100 microm) as well as apyrase (5 units/ml) attenuated hypoxia- and ATP-induced

39 means of the following secreted exoenzymes: apyrase, 5'-nucleotidase, and adenosine deaminase.

40 Apyrase (500 units ml(-1)), an ATP hydrolysing enzyme, d

41 amin (100 microm), PPADS (20-50 microm), and apyrase (80 U/ml), in contrast, substantially reduced wa

42 roduced when the cells were coincubated with apyrase, a highly effective ATP scavenger.

43 Our results demonstrate that salivary apyrase, a known inhibitor of platelet aggregation, inte

44 Apyrase, a nucleotide-degrading enzyme, suppressed and a

45 also attenuated in monocytes incubated with apyrase, a potent scavenger of extracellular ATP.

46 high pressure liquid chromatography and the apyrase active band from chromatofocusing gels.

47 ceptors, being 93 microm;(d) measured latent apyrase activities in the above vesicles, suggesting the

48 Chemicals that suppress apyrase activity also inhibit gravitropic curvature and,

49 detected, brains from these mice lacked both apyrase activity and CD39 immunoreactivity.

50 and that only a fully glycosylated CD39 has apyrase activity and is localized at the cell surface.

51 B regulates SbtA activity, in which both the apyrase activity and its redox regulation play a central

52 TP into the growth medium and suppression of apyrase activity by antiapyrase antibodies or by inhibit

53 Finally, we show that CD39 indeed has ecto-apyrase activity by expression in COS-7 cells.

54 he cytoplasmic domain of Ynd1p regulates its apyrase activity in the Golgi lumen.

55 his report, we show that an ecto-(Ca2+,Mg2+)-apyrase activity is present on EBV-transformed B cells,

56 hese findings, we observed that the salivary apyrase activity of L. longipalpis is indeed similar to

57 A similar increase in the apyrase activity of Ynd1p was found in a vma1Delta mutan

58 coincidence between CD39 expression and ecto-apyrase activity on immune cells suggests that CD39 may

59 igen 39 (CD39), a molecule now known to have apyrase activity.

60 h changes of the R-loop redox state modulate apyrase activity.

61 idates the unusual calcium-dependence of its apyrase activity.

62 ing cells with extracellular ATP scavengers [apyrase + adenosine deaminase] versus 95 +/- 12% survivi

63 ilies of phosphatases (inactive), CD-39-type apyrase, adenosine deaminases, and esterases.

64 Histologic studies showed that apyrase administration abrogated local platelet aggregat

65 A single dose of apyrase administration before IRI protects from both acu

66 Apyrase administration significantly prolonged graft sur

67 d the function of Anopheles gambiae salivary apyrase (AgApyrase) in regulating hemostasis in the mosq

68 e motor, while ATP, ATP gamma S, AMPPNP, and apyrase all induce a shift toward tighter binding states

69 Strikingly, deciliation, BAPTA-AM, and apyrase also blocked the flow-dependent increase in endo

70 Apyrase (an agent of ADP degradation), suramin (a genera

71 Suramin, apyrase (an ATP-hydrolyzing enzyme), and TTX substantial

72 In addition, the inhibitory effects of apyrase, an ADP scavenger, on spreading and tyrosine pho

73 Local injection of apyrase, an ATP-hydrolyzing enzyme, inhibited cell recru

74 lasts in the absence of ATP was inhibited by apyrase, an ecto-ATPase and by suramin, an antagonist of

75 TP and ADP were abolished in the presence of apyrase, an ectonucleotidase.

76 ne marrow reconstitution and provision of an apyrase, an enzyme that hydrolyzes nucleoside tri- and d

77 d [Ca(2+)]i rise was partially attenuated by apyrase, an enzyme that inactivates extracellular ATP, a

78 P2 antagonists or apyrase, an enzyme which hydrolyzes nucleotides includin

79 Moreover, using apyrase, an extracellular ATP scavenger, we showed that

80 ch makes them susceptible to ATP-hydrolyzing apyrase and ACC(1-13)K(n)-digesting proteinase K.

81 Apyrase and antagonists of platelet purinergic receptors

82 nzymatic degradation of extracellular ATP by apyrase and blockade of P2Y-purinoceptors by suramin or

83 lso reduced significantly in the presence of apyrase and connexin channel inhibitors.

84 chromatography of detergent-solubilized ecto-apyrase and cross-linking of membrane-bound ecto-apyrase

85 latory volume decrease which was impaired by apyrase and facilitated by ATP or UTP.

86 ymes that use acid-labile substrates such as apyrase and glutamine synthetase.

87 e stomach 80-kDa protein isolated is an ecto-apyrase and is related to both the chicken liver and ovi

88 ts of increased CD39 activity (using soluble apyrase and mice expressing human CD39 transgene) on acu

89 , including potato tuber (Solanum tuberosum) apyrase and parasite ecto-ATPase, are not affected by de

90 nreported (to our knowledge) linkage between apyrase and phosphate transport.

91 nsients in submucous neurons were reduced by apyrase and prevented by blocking the P2Y1 R with MRS 21

92 ere induced by the soluble ecto-nucleotidase apyrase and the P2 receptor inhibitor suramin.

93 ast to the enzymatically deglycosylated ecto-apyrase and the tunicamycin-treated ecto-apyrase.

94 displacement was reversed by the addition of apyrase and was specific to chromatin templates.

95 robenecid, and (10)Panx1), ectonucleotidase (apyrase), and purinergic receptor inhibitors (suramin an

96 ation (5 mm EGTA), ATP depletion (4 units/ml apyrase), and the protein kinase C (PKC) inhibitors chel

97 his increase in motility is not sensitive to apyrase, and apyrase does not detectably inhibit healing

98 adation was significantly inhibited by EDTA, apyrase, and the proteasome inhibitors hemin and MG132.

99 ity within xenografts by infusion of soluble apyrases, and thereby validate the importance of local A

100 Anti-ecto-apyrase antibody detected a single 80-kDa band (putative

101 elements from the Maltase-like I (MalI) and Apyrase (Apy) genes were cloned so as to direct the expr

102 lases), and two nearly identical Arabidopsis apyrases, APY1 and APY2, appear to share this function.

103 Two closely related apyrases (APYs) in Arabidopsis (Arabidopsis thaliana), A

104 Apyrases are non-energy-coupled nucleotide phosphohydrol

105 sults suggest that the ecto-ATPases and ecto-apyrases are part of, or closely related to, the actin s

106 , and this [Ca2+]i increase was inhibited by apyrase as well.

107 ressed the protein and message level of both apyrases at least as rapidly as it inhibited hypocotyl g

108 we previously identified two highly similar apyrases, AtAPY1 and AtAPY2.

109 y of spontaneous wave generation by 53%, and apyrase (ATP-hydrolyzing enzyme) reduces frequency by 95

110 tion of exogenous potato (Solanum tuberosum) apyrase (ATPase) decreased ROS activity, suggesting that

111 zation of extracellular ecto-ATPase and ecto-apyrase (ATPDase) in adult chicken gizzard and stomach b

112 ces confirmed that pretreatment with oATP or apyrase attenuated cytokine-mediated induction of this t

113 Suramin, Cibacron blue 3GA, and apyrase attenuated hypoxia-induced ERK1/2 activation and

114 terminal sequence of the 80-kDa stomach ecto-apyrase band (which reacted with anti-ecto-ATPDase antib

115 , 18-alpha-glycyrrhetinic acid (18alpha-GA), apyrase, BCTC, AMG 9810, or AMTB.

116 the prostaglandin E(1) or the ADP scavenger apyrase but was prevented by the divalent cation chelato

117 icant sequence similarity to any other known apyrases, but homologous sequences have been found in th

118 hibited by removal of extracellular ATP with apyrase, by inhibition of the P2X(7) receptor with A4380

119 +) that was blocked by MK-801, by the ATPase apyrase, by the P2Y(1) receptor antagonist MRS2179 and b

120 a presented show that expression of the GS52 apyrase can enhance nodulation in L. japonicus and point

121 xy-D-glucose with oligomycin or perfusion of apyrase), can be restored with phosphatidylinositol 4,5-

122 These results clearly suggest that GS52 ecto-apyrase catalytic activity is critical for the early B.

123 substrate specificity characteristic of the apyrase category of phosphohydrolases, and its sequence

124 alysis revealed an increase in CD73 and ecto-apyrase CD39 in hypoxic epithelial cells.

125 Regulatory T cells (known as "Treg") express apyrases (CD39) and ecto-5'-nucleotidase (CD73) and cont

126 esults were obtained with another human ecto-apyrase, CD39, suggesting that the importance of glycosy

127 We have established a new "apyrase chase" strategy that uncouples priming from chai

128 This apyrase cluster was mapped to linkage group seven.

129 7 to alanine yielded a poorly expressed ecto-apyrase completely devoid of nucleotidase activity.

130 We show that mutations in apyrase conserved region 1 (ACR1) have two dramatically

131 cted mutagenesis of conserved amino acids in apyrase conserved regions (ACR) I and IV.

132 2) contains conserved motifs including five apyrase conserved regions (ACRs) and four conserved regi

133 ructural basis for the importance of several apyrase conserved regions for the nucleotidase activitie

134 contain two transmembrane domains and five "apyrase conserved regions" (ACR) within a large extracel

135 Four highly conserved regions (apyrase conserved regions, ACRs) have been identified in

136 ur of the conserved sequences, designated as apyrase conserved regions, are present in both ecto-ATPa

137 tion sites, conserved cysteine residues, and apyrase conserved regions.

138 were generated by replacement of exons 4-6 (apyrase-conserved regions 2-4) with a PGKneo cassette.

139 r APY1 and APY2 show that expression of both apyrases correlates with conditions that favor stomatal

140 psis, suggested that the expression of these apyrases could influence auxin transport.

141 iating platelet aggregation (i.e., Aegyptin, apyrase, D7) represent functional redundancy.

142 by canine kidney cells with the nucleotidase apyrase decreases basal arachidonic acid release and cAM

143 inergic P2-receptor inhibitor or addition of apyrase demonstrated a requirement for nucleotide tripho

144 Here, we reveal an unusual ATP/ADP apyrase (diphosphohydrolase) activity of SbtB that is co

145 in motility is not sensitive to apyrase, and apyrase does not detectably inhibit healing of wounds in

146 ontains one large NH(2)-terminal hydrophilic apyrase domain, one COOH-terminal hydrophilic domain, an

147 at mosquitoes ingest a substantial amount of apyrase during blood feeding, which reduces coagulation

148 ta suggest a critical role for the GS52 ecto-apyrase during nodulation.

149 The data suggest a role for apyrases early in the nodulation response before the inv

150 tes, but not monophosphates, thus displaying apyrase (EC 3.6.1.5) activity, was purified from salivar

151 We used apyrase, EDTA and Dowex-1 to prepare actin that is stabl

152 d by the gene uda-1, whereas the other is an apyrase encoded by the gene ntp-1.

153 es the role of extracellular nucleotides and apyrase enzymes in regulating stomatal aperture.

154 idase 1 (hSCAN-1) represents a new family of apyrase enzymes that catalyze the hydrolysis of nucleoti

155 The glycosylated ecto-apyrase exists as a homodimer in situ as assessed by bot

156 ression changes that occur in seedlings when apyrase expression is suppressed were assayed by microar

157 onstruct was measured in primary roots whose apyrase expression was suppressed either genetically or

158 arthropods, while members of the Cimex-type apyrase family have been recruited at least twice.

159 structures of a human enzyme of the soluble apyrase family in its apo state and bound to a substrate

160 hlights a pivotal role for mosquito salivary apyrase for regulation of hemostasis in the mosquito blo

161 (hSCAN-1) is the human homologue of soluble apyrases found in blood-sucking insects.

162 ed cDNA clone recognized both C. lectularius apyrase fractions eluted from a molecular sieving high p

163 An apyrase from pea (Pisum sativum) complements a yeast (Sa

164 wever, the recent cloning of an ecto-ATPase (apyrase) from potato tubers provides a new opportunity t

165 The human ecto-apyrase gene family consists of five reported members (C

166 Transgenic plants overexpressing an apyrase gene had reduced O2- production in response to a

167 is thaliana of either psNTP9, the garden pea apyrase gene, or AtPgp1, the A. thaliana homolog of the

168 Four putative apyrase genes were identified from the model legume Medi

169 ge group J was found to contain at least two apyrase genes.

170 tant role for the soybean (Glycine max) ecto-apyrase GS52 in rhizobial root hair infection and root n

171 The soybean apyrase, GS52, was previously characterized as an early

172 s and etiolated hypocotyls overexpressing an apyrase had faster growth rates than wild-type plants.

173 C. lectularius apyrase has no significant sequence similarity to any ot

174 The cDNA encoding a human ecto-apyrase (HB6), predicted to have seven N-linked glycosyl

175 i bearing an arabinose-inducible periplasmic apyrase hydrolyzing ATP to be released.

176 In addition, when ATP was scavenged with apyrase, hyperoxia-induced inflammasome activation was s

177 larity to: (i) the bed bug Cimex lectularius apyrase, (ii) a 5'-nucleotidase/phosphodiesterase, (iii)

178 Removal of the extracellular ATP by apyrase in CM abolished this effect, suggesting the invo

179 ctions, but the antibody did not detect ecto-apyrase in immunolabeled gizzard cryosections.

180 ngipalpis is indeed similar to that of Cimex apyrase in its metal requirements.

181 Overexpression of the GS52 ecto-apyrase in Lotus japonicus increased the level of rhizob

182 reatment with BAPTA-AM, or upon inclusion of apyrase in the perfusion medium.

183 ate hydrolase activity, we employed a potato apyrase in vitro and in vivo, as well as targ-CD39 and a

184 ior data indicate that the expression of two apyrases in Arabidopsis (Arabidopsis thaliana), APY1 and

185 Despite the redundancy of the two apyrases in rescue potential, transmission analyses sugg

186 body detected a single 80-kDa band (putative apyrase) in Western blots of both chicken gizzard membra

187 ase and cross-linking of membrane-bound ecto-apyrase, in contrast to the enzymatically deglycosylated

188 Likewise, treatment with the ATPase apyrase, in the presence of the adenosine A1 and A2 rece

189 denosine A1 receptor blockade and reduced by apyrase inactivation of nucleotidases, P2 receptor antag

190 ed homology with other ecto-ATPases and ecto-apyrases, including those from the parasitic protozoan T

191 is initial dose was followed by a continuous apyrase infusion (8.0 U/kg/hr) directly into the graft a

192 ARL67156 augmented, and apyrase inhibited, stroking-evoked Isc responses.

193 Apyrase inhibition by small molecule inhibitors reversed

194 Apyrase, inhibition of P2 receptors, or inhibition of p3

195 Treatment of untransformed plants with an apyrase inhibitor increased their sensitivity to the sam

196 Ecto-apyrase is enriched in brain postsynaptic density membra

197 esent throughout the brain suggest that ecto-apyrase is involved in regulating synaptic transmission

198 emplified by the endothelial CD39 (NTPDase1) apyrase, is a 38 kDa monomeric enzyme capable of hydroly

199 rthermore, we show that CD39-L4 is an E-type apyrase, is dependent on calcium and magnesium cations,

200 y dithiols, NTPase is one of the most potent apyrases known to date, but its physiological function r

201 d characterization of a novel mammalian endo-apyrase (LALP1) in human and mouse.

202 Functional apyrase levels in vivo were assessed by the capacity of

203 ates that the catalytic activity of the GS52 apyrase, likely acting on extracellular nucleotides, is

204 predicted isoelectric point of the putative apyrase matches the value found for Lutzomyia salivary a

205 c extensions whose lengths are controlled by apyrase-mediated substrate degradation.

206 ing ATPase activity by injecting recombinant apyrase mitigates AIHA development and significant CD39S

207 When scavenging phosphate from xATP, apyrase mobilizes the gamma-phosphate without promoting

208 of mRNA expression of the other two putative apyrases, Mtapy2 and Mtapy3, was unaffected by rhizobial

209 ed that the constitutive expression of a pea apyrase (Nucleoside triphosphate, diphosphohydrolase) ge

210 on of two Arabidopsis (Arabidopsis thaliana) apyrase (nucleoside triphosphate-diphosphohydrolase) gen

211 We also partially purified the ecto-apyrase of chicken stomach, an 80-kDa membrane glycoprot

212 TPD1, also known as CD39) is a vascular ecto-apyrase on the surface of leukocytes and the endothelium

213 The effect of mutant apyrase on the transmission through the female gametophy

214 Removal of extracellular ATP by apyrase or alkaline phosphatase treatment, inhibition of

215 When extracellular ATP was hydrolyzed by apyrase or ATP/P2 receptors were blocked, injury-induced

216 d by either extracellular ATP depletion with apyrase or blockade of P2 receptors with suramin.

217 n be inhibited by the ATP-hydrolyzing enzyme apyrase or by blockers of G protein-coupled purinergic r

218 Luminal apyrase or suramin blocked the effects of forskolin but

219 icturition because intravesical perfusion of apyrase or the ecto-ATPase inhibitor ARL67156 altered re

220 Enzymatic removal of ATP and UTP (by apyrase or the expression of ectopic CD39) abrogated the

221 by the serosal, but not mucosal, addition of apyrase or the purinergic receptor antagonist PPADS.

222 on with the nonspecific pyrophosphohydrolase apyrase or with hexokinase and was specifically lost by

223 s, producing either monomers of the peptide (apyrase) or free ATP released together with cleaved-off

224 The slow waves were not affected by apyrase, or by the P2 receptor agonists suramin (150 mum

225 O mutation is fully penetrant and shows that apyrases play a crucial role in pollen germination.

226 related proteins," PPTSP42 and PPTSP44, and "apyrase," PPTSP36, are the proteins responsible for the

227 nding to the N-terminal sequence of purified apyrase produced a probe that allowed identification of

228 ading endogenous spinal ATP by administering apyrase produces a reduction in withdrawal behaviors.

229 ata demonstrate that local administration of apyrase prolonged discordant xenograft survival.

230 t express luciferase (LUC) from the mosquito Apyrase promoter were intrathoracically inoculated with

231 beta-glucuronidase staining in the pollen of apyrase promoter:beta-glucuronidase fusion transgenic li

232 and fly species such as the yellow, SP15 and apyrase protein families.

233 We have shown that ecto-apyrase protein is expressed in primary neurons and astr

234 mmunohistochemical studies showing that ecto-apyrase protein is widely distributed in rat brain, as i

235 report that overexpression of either MDR or apyrase proteins resulted in increased resistance to her

236 Soluble apyrase reduced acute renal injury at 24 hours and renal

237 Soluble apyrase reduced renal ATP, adenosine diphosphate, and ad

238 by the P2Y1 blockers, MRS2176, suramin, and apyrase, reduces Ca(2+) transients and retards INP migra

239 mutagenesis within the four highly conserved apyrase regions of solCD39.

240 The two transmembrane domains of CD39 ecto-apyrase regulate the formation of fully active homotetra

241 mmunolocalization of this active mutant ecto-apyrase revealed a cellular pattern similar to that of t

242 Incubating lingual slices with apyrase reversibly blocked cell-to-cell communication be

243 The ATP/ADPase apyrase reversibly inhibited the [Ca2+]i rise induced by

244 onized extension due mainly to a decrease in apyrase's efficiency in degrading excess nucleotides pro

245 inhibitor A740003 or the depletion of ATP by apyrase selectively abrogated ATP-induced, but not oxala

246 immunoreactive material detected by the anti-apyrase serum.

247 approach, we were able to redesign the human apyrase so as to enhance its ADPase activity by more tha

248 to hydrolysis of extracellular ATP levels by apyrase (soluble NTPDase).

249 rmination, confirming that the phenotype was apyrase specific.

250 In addition, apyrase strongly inhibited influenza-specific T-cell res

251 Computer modeling of the GS52 apyrase structure identified key amino acid residues imp

252 noid waves were blocked by either octanol or apyrase, suggesting that propagation might occur either

253 lts indicate that a critical step connecting apyrase suppression to growth suppression is the inhibit

254 on by azide, a more potent inhibitor of ecto-apyrases than ecto-ATPases.

255 Hematophagous arthropods secrete a salivary apyrase that inhibits platelet activation by catabolizin

256 On the other hand, apyrase, the P2Y12 receptor antagonist, AR-C67085, and i

257 was studied, using the ATP hydrolyzing agent apyrase, the purinergic receptor agonist PPADS, the calc

258 to the secretion of the ATP-degrading enzyme apyrase, thus creating engineered yeast probiotics capab

259 P) essentially converted the eNTPDase-3 ecto-apyrase to an ecto-ATPase (eNTPDase-2), mainly by decrea

260 n E-type ATPase essentially converts an ecto-apyrase to an ecto-NTPase.

261 The ability of apyrase to block chemokine-dependent aggregation or adhe

262 by pyrophosphate in vitro and by addition of apyrase to degrade residual NTPs during NET-seq sample p

263 Mice were treated with anti-IL-33 or apyrase to neutralize or block IL-33 release.

264 Pre-photolysis force was minimized using apyrase to remove contaminating ATP and ADP.

265 ell death, and addition of the ATP scavenger apyrase to remove extracellular ATP released during Hst

266 evealed that two such mutants do not express apyrases to any detectable level.

267 Experiments in apyrase-treated cells suggested involvement of a P2Y rec

268 Apyrase treatment also depressed DOR stimulation of intr

269 Apyrase treatment of translation mixtures after synthesi

270 pletion of extracellular nucleotides with an apyrase treatment strongly potentiated cell death and pr

271 Apyrase treatment substantially reduced the basal profib

272 nt protein kinase kinase (CaMKK) inhibition, apyrase treatment, G(q/11) antagonism, and blockade of P

273 ecific activity of the purified stomach ecto-apyrase was 75,000 micromol of Pi/mg of protein/h, and t

274 ic cardiac xenografting from guinea pigs and apyrase was administered intravenously (200 U/kg) as a s

275 The ecto-apyrase was also expressed in the presence of tunicamyci

276 The purified C. lectularius apyrase was an acidic protein with a pI of 5.1 and molec

277 in 70/sugar kinase superfamily, a human ecto-apyrase was analyzed by site-directed mutagenesis of con

278 Surprisingly, apyrase was more effective than prostacyclin and NO at l

279 In adult chicken stomach, the ecto-apyrase was observed at the apical membrane of the gland

280 of the cDNA clone with native C. lectularius apyrase was proved by immunological testing and by expre

281 motors, the nucleotide-free state induced by apyrase was the strongest binding (K(kin)d approximately

282 Apyrase was used to hydrolyze extracellular ATP, and sur

283 ther methods, the highest expression of both apyrases was in rapidly growing tissues and/or tissues t

284 A human brain E-type ATPase (HB6 ecto-apyrase) was subjected to site-directed mutagenesis to a

285 GS52, a soybean (Glycine soja) ecto-apyrase, was previously shown to be induced very early i

286 g CD39 activity with ARL67156 (inhibitor) or apyrase were also observed.

287 etic analyses of three families of arthropod apyrases were used to reconstruct the evolutionary relat

288 toenzyme ATP-diphosphohydrolase (ATPDase; an apyrase), which exerts an important thromboregulatory fu

289 In the presence of apyrase, which hydrolyzes ATP to AMP, the effect of LL37

290 This apyrase, which is distinct from the eNTPDases exemplifie

291 newly discovered human analogue of a bed bug apyrase, which we named hSCAN-1 for human soluble calciu

292 utionary relationships of salivary-expressed apyrases, which have an anti-coagulant function in blood

293 fected COS-7 cells secreted a Ca2+-dependent apyrase with a pI of 5.1 and immunoreactive material det

294 Replacing apyrase with a washing step for removal of excess nucleo

295 ryptophan 459 to alanine resulted in an ecto-apyrase with enhanced NTPase activity, but diminished ND

296 LALP1 indicated that LALP1 is indeed an endo-apyrase with substrate preference for nucleoside triphos