ライフサイエンスコーパス: arabinan

1 ybrid was of AG kind rather than LAM type of arabinan.

2 d that also exuded a guttation fluid rich in arabinan.

3 es within the secretome of A. niger grown on arabinan.

4 to the hexasaccharide fragment of cell wall arabinan.

5 ved in other ABNs with preference for linear arabinan.

6 to a trisaccharide fragment of mycobacterial arabinan.

7 mplicated in the biogenesis of the cell wall arabinan.

8 model to study the biosynthesis of cell wall arabinan.

9 A), resulted in a complete loss of cell wall arabinan.

10 after extensive loss of the highly branched arabinans.

11 sults are consistent with NaARADL1 having an arabinan (1,5)-alpha-arabinosyltransferase activity.

12 ults show that this novel pyranosic sulfated arabinan Ab1 exerts its anticoagulant activity by a mech

13 A highly sulfated 3-linked beta-arabinan (Ab1) with arabinose in the pyranose form was o

14 erified rhamnogalacturonan I and 1,5-, 1,3,5-arabinan and 4-O-Me-glucuronoxylan polysaccharides.

15 fraction containing a linear (1-->5)-alpha-L-arabinan and a linear (1-->3)-(1-->4)-alpha-D-glucan was

16 Colocalizing pea-fiber arabinan and another polysaccharide (glucomannan) on the

17 Arabinan and arabinogalactan were also found in all the

18 ovalently linked complex of mycolic acids, D-arabinan and D-galactan (mycolylarabinogalactan, mAG), w

19 ramolecule that contains rhamnogalacturonan, arabinan and galactan as structural elements.

20 arabinose side chains, and with 1,5-, 1,3,5-arabinan and galactan polysaccharides.

21 cellulose, highly methylated pectins rich in arabinan and galactan side chains, and xyloglucan.

22 f hairy region that is heavily branched with arabinan and galactan side chains.

23 ated and xylosylated galactose backbone with arabinan and mannoglucuronan branches.

24 virulence factors; biosynthesis of cell-wall arabinan and peptidoglycan; DNA repair; sterol metabolis

25 In contrast, the binding of LM16 to branched arabinan and to cell walls is increased by arabinofurano

26 ranosidase activity, we generated enriched D-arabinan and used it to identify a strain of Dysgonomona

27 l antibodies that are directed to 1,5-linked arabinans and related polymers.

28 llulolytic polysaccharides, including xylan, arabinan, and galactan.

29 ure that is rich in pectic homogalacturonan, arabinan, and xyloglucan.

30 PPS, including sugar beet pectin/arabinan, apple/citrus pectin and potato galactan, were

31 i)cellulolytic enzymes and reduced growth on arabinan, arabinogalactan and xylan.

32 , it was shown that rhamnogalacturonan I/II, arabinan, arabinogalactan types I and II and xyloglucan

33 to ARABINAN DEFICIENT1 (AtARAD1), a presumed arabinan arabinosyltransferase from Arabidopsis (Arabido

34 grown M. leprae is apparently simpler in its arabinan architecture with a high degree of exposed, non

35 ethyl-esterified pectic homogalacturonan and arabinan are abundant in syncytial cell walls; galactan

36 homogalacturonan and pectic (1-->5)-alpha-L-arabinan are present in cotyledon cell walls throughout

37 art from critically showing that the smaller arabinans are mostly devoid of the linear terminal motif

38 d l-arabinosyl residues and longer chains of arabinan as demonstrated with the use of arabinan-degrad

39 Analysis of the number of arabinans attached to the mannan core of LM in two other

40 idues and side chains that include alpha-1,5-arabinans, beta-1,4-galactans, and arabinogalactans.

41 Identification of genes involved in arabinan biosynthesis entailed the use of ethambutol (EM

42 fundamental differences, with both cell wall arabinan biosynthesis in mycobacteria, and periplasmic g

43 An Emb-sensitive cell-free assay for arabinan biosynthesis shows that overexpression of embAB

44 inhibition leads to inhibition of cell wall arabinan biosynthesis.

45 osis drug ethambutol inhibits other steps in arabinan biosynthesis.

46 alpha-L-arabinofuranoside, arabinoxylan, and arabinan but not for p-nitrophenyl alpha-L-arabinopyrano

47 taiotaomicron experiences the BT0366 inducer arabinan but not when grown in the presence of glucose.

48 are known to be involved in the synthesis of arabinan but their biochemical functions are not underst

49 were formed and initiation and elongation of arabinan can occur either on the 5-arm or 3-arm of the b

50 us, these studies suggest that only a single arabinan chain attached near the middle of the mannan co

51 For the first time a full range of arabinan chains as large as 18-22 Araf residues and beyo

52 sidues, (ii) the exact site of attachment of arabinan chains in AG, and (iii) DPA is the only Araf su

53 mycolyl arabinogalactan highlighted by three arabinan chains of 31 residues each.

54 branching structure of LM and the number of arabinan chains on the mannan backbone in LAM remain.

55 icotiana alata pollen tubes contain a linear arabinan composed of (1,5)-alpha-linked arabinofuranose

56 D-Arabinans, composed of D-arabinofuranose (D-Araf), domin

57 nanases with different preferences for the D-arabinan-containing cell wall components arabinogalactan

58 When chondroitin sulfate is added to arabinan-containing cultures, BT0366 phosphorylation and

59 glycans, including trehalose glycolipids and arabinan-containing glycoconjugates.

60 To illustrate one approach, an arabinan-containing polysaccharide was isolated from pea

61 In contrast to the decrease in arabinan content, the loss of the rhamnogalacturonan I b

62 age, and the distal cell walls had decreased arabinan content.

63 Here, we identify enzymes that cleave the D-arabinan core of arabinogalactan, an unusual component o

64 ghly branched arabinofuranose residue in the arabinan core.

65 LAM) with a marked reduction of their linear arabinan (corresponding mainly to the inner Araf-alpha(1

66 -like sequence, which we have named N. alata ARABINAN DEFICIENT-LIKE1 (NaARADL1), accumulate in vario

67 A sequence related to ARABINAN DEFICIENT1 (AtARAD1), a presumed arabinan arabi

68 of GH43 ABNs and provide new strategies for arabinan degradation.

69 entify a strain of Dysgonomonas gadei as a D-arabinan degrader.

70 of arabinan as demonstrated with the use of arabinan-degrading enzymes.

71 l-recognized importance of the mycobacterial arabinan, delineation of the arabinosylation process has

72 syl donors whose addition restores cell wall arabinan, demonstrating that non-natural glycolipids can

73 mb also affected the general biosynthesis of arabinan destined for both AG and LAM, resulting in seve

74 iously unknown forms of LAM with a truncated arabinan domain and 3-linked oligomannoside chains, they

75 a pivotal role in the biosynthesis of these arabinan domains by catalyzing the addition of beta-(1 -

76 LAM), two complex polysaccharides containing arabinan domains essential for maintaining cell wall str

77 donor to then be used in the buildup of the arabinan domains of AG and LAM.

78 here SucT, that adds succinyl groups to the arabinan domains of both arabinogalactan (AG) and lipoar

79 cifically, we show that a short-chain linear arabinan epitope associated with the presence of rhamnog

80 ating the recognition of a longer linearized arabinan epitope.

81 Carbohydrate-specific antibodies detected arabinan epitopes at the tip and along the shank of N. a

82 ng to the novel accumulation of longer-chain arabinan epitopes in guard cell walls led to an increase

83 to the three major RG-I structural elements (arabinan, galactan and the rhamnogalacturonan backbone)

84 olymers and increasing the extractability of arabinans, galactans, arabinogalactan proteins and manna

85 of these steps, the lipid-linked-LU-galactan-arabinan has been partially characterized in terms of it

86 etails of the mycolic acid attachment to the arabinan have remained unknown.

87 oelectric focusing based apparently on their arabinan heterogeneity.

88 tic polysaccharide rhamnogalacturonan I, the arabinan in N. alata pollen tubes is considered free, as

89 RADL1 in Arabidopsis led to plants with more arabinan in their walls and that also exuded a guttation

90 een guard cell size and DNA content, lack of arabinans in cell walls, and perpetually open pores are

91 acterial metabolism of structurally distinct arabinans in different human diet contexts.

92 The D-arabinans in Mycobacterium are essential, extraordinaril

93 f endo- and exo-acting enzymes that cleave D-arabinan, including members of the DUF2961 family (GH172

94 The mycobacterial arabinan is an elaborate component of the cell wall with

95 itional features of the plants, such as high arabinan levels in beet and high galactan levels in carr

96 acterial cell wall, including peptidoglycan, arabinan, linker unit galactan, and lipoarabinomannan.

97 the context of truncated or less elaborated arabinan, may contribute to selective recognition by T c

98 viously demonstrated selective mycobacterial arabinan modification by biosynthetic incorporation usin

99 netic and biochemical data showing that wall arabinans modulate guard cell flexibility and can be use

100 Less sulfated arabinans obtained from the same seaweed have less or no

101 tive loss of highly branched (1-->5)-alpha-L-arabinans occurs after ripening and in advance of the lo

102 The selective loss of highly branched arabinans occurs during the overripening of apples of fo

103 usly we have shown that the synthesis of the arabinan of AG is affected by embA or embB disruption.

104 for the polymerization of arabinose into the arabinan of arabinogalactan, and that overproduction of

105 mannose residues; in some other species, the arabinan of LAM is not capped or is capped with inositol

106 mass spectrometry analyses revealed that the arabinan of the 'LAM' formed in the hybrid was of AG kin

107 lose (MTX), adorned the nonreducing terminal arabinan of these LAM species.

108 Emerging data indicate that the arabinans of AG and LAM are distinguished by virtue of t

109 previously shown to inhibit the synthesis of arabinans of both the cell wall arabinogalactan (AG) and

110 is involved in the biosynthesis of singular arabinans of LAM.

111 The arabinans of the mycobacterial cell wall are key structu

112 rsus unfractionated pea fiber and sugar beet arabinan, on a human gut bacterial strain consortium in

113 y 62 (GH62), hydrolyzes arabinoxylan but not arabinan or arabinogalactan.

114 oside hydrolases with activity against the D-arabinan or D-galactan components of arabinogalactan.

115 ilization at RT than the enzymes involved in arabinan or galactan disassembly.

116 nosyltransferases (Emb) that are involved in arabinan polymerization.

117 We demonstrate that arabinans present in different constituents of plant cel

118 In addition, an inner arabinan region of 14 linear alpha-1,5 arabinofuranosyl

119 ed an endogenous arabinase to solubilize the arabinan region of the cell wall and have shown using ma

120 rabinofuranoside backbone of plant cell wall arabinans releasing arabino-oligosaccharides and arabino

121 The unbranched (1-->5)-linked arabinans remain associated with the major pectic polyme

122 a), showed that arabinogalactan-proteins and arabinans represent substantial portions of the Golgi-re

123 berculosis, and modification of the terminal arabinan residues of this compound with mannose caps (pr

124 rious polysaccharides (water-soluble pectin, arabinan, rhamnogalacturonan I, arabinoxylan, xyloglucan

125 bodies binds strongly to isolated sugar beet arabinan samples in ELISAs.

126 beta1,2-arabinobiose (Ara2) from sugar beet arabinan (SBA), and beta1,2-Ara2 and alpha-1,2-galactoar

127 -galactan chain and in the proportion of the arabinan side chain.

128 experimental support for the conclusion that arabinan side chains of pectin modulate guard cell wall

129 The results reveal the high debranching of arabinan side chains of RG I as compared to the galactan

130 ogalacturonans and rhamnogalacturonan-I with arabinan side-chains ( approximately 55-60%), as well as

131 High arabinose level suggests that long arabinan side-chains maintain cell wall flexibility in w

132 cant revision of the structural model of LAM-arabinan since its first description a decade ago but al

133 nd relate both to the probable processing of arabinan structural elements and the differing mechanica

134 ibodies that can detect differing aspects of arabinan structures within cell walls.

135 of EmbC resulted in marked reduction of LAM-arabinan synthesis and accumulation of an unknown interm

136 ing arabinose, perhaps in the early stage of arabinan synthesis in lipoarabinomannan.

137 [14C]ribosyl-P-P as a donor of [14C]Araf for arabinan synthesis, we now demonstrate sequential synthe

138 ow for the presentation of a unique model of arabinan synthesis.

139 sis agent that is known to inhibit cell wall arabinan synthesis.

140 al inhibition of the synthesis of the linear arabinan terminal motif, which constitutes a substantial

141 of the mannose residues on the non-reducing arabinan termini and the basis of much of the interactio

142 data clearly indicate the importance of the arabinan termini arrangements for the antigenicity of LA

143 ich constitutes a substantial portion of the arabinan termini in LAM but not of AG.

144 np motifs that functionalize the nonreducing arabinan termini of LAM (ManLAM) in Mycobacterium tuberc

145 but a single Manp residue on the nonreducing arabinan termini of LAM and also a complete absence of a

146 In M. tuberculosis, the non-reducing arabinan termini of the LAM are capped with alpha1-->2 m

147 ined concanavalin A reactivity, and that the arabinan termini were capped with a single mannose resid

148 l anchor followed by a mannan followed by an arabinan that may be capped with various motifs includin

149 transferases involved in the biosynthesis of arabinans unique to the mycobacterial cell wall.

150 inked d-Araf units observed in wild type LAM-arabinan was largely retained in the proline motif mutan

151 id showed that a soluble, linear alpha-(1,5)-arabinan was the most abundant polymer present.

152 Using a C. glutamicum mutant that lacks arabinan, we identified synthetic glycosyl donors whose

153 that the fraction PTW consisted of a linear arabinan with (1-->5)-linked alpha-l-arabinofuranosyl un

154 dicate the antibodies bind differentially to arabinans with the binding of LM6 and LM17 being effecti

155 h, cellulose, beta-glucan, mannan, galactan, arabinan, xylan, xyloglucan, chitin) were successfully q