ライフサイエンスコーパス: assay system

1 antly lower than those estimated in agarised assay system.

2 he prm240 promoter, measured in an uncoupled assay system.

3 expressed with wild-type L in the minigenome assay system.

4 n secretion was measured with a fluorescence assay system.

5 and the appropriateness of animal caps as an assay system.

6 and product are analyzed using the optimized assay system.

7 or phase, demonstrating the validity of the assay system.

8 micromolar concentration range in a cellular assay system.

9 oilage bacteria, both in agarised and liquid assay system.

10 ubstrate were added to a fluorogenic peptide assay system.

11 ct on transferrin endocytosis using the same assay system.

12 ncer-blocking capabilities using a transgene assay system.

13 DA-MB-231 breast cancer cells in a coculture assay system.

14 ic agonist, was measured with a fluorescence assay system.

15 oligosaccharides and the sensitivity of the assay system.

16 eviously demonstrated in a purified in vitro assay system.

17 recognition complex (ORC) using an in vitro assay system.

18 ate group from mature lipid A in an in vitro assay system.

19 e easily adapted to any bicistronic reporter assay system.

20 nd C with the TLR5 receptor using a reporter assay system.

21 ein has been studied in an in vitro adhesion assay system.

22 ation was evaluated using a defined in vitro assay system.

23 yeast (Saccharomyces cerevisiae) two-hybrid assay system.

24 ds caused some opiate activity by at least 1 assay system.

25 17, and tumor necrosis factor-alpha using an assay system.

26 ted ATPase activity in an in vitro transport assay system.

27 MO-targeted ubiquitin ligase activity in our assay system.

28 minescence output in a discontinuous coupled assay system.

29 ble when the human plasma was present in our assay system.

30 OVID-19 or vaccine recipients in a cell-free assay system.

31 hen implemented within a competitive binding assay system.

32 vitro mouse embryonic stem cell (ESC) clonal assay system.

33 ith ZEB1 on the Zp ZV element in a cell-free assay system.

34 with specific virulent strains using several assay systems.

35 FX to attenuate FX activation in lipid-free assay systems.

36 for their reduced catalytic activity in all assay systems.

37 cation of positive controls for use in novel assay systems.

38 efficient kinase activity-dependent cellular assay systems.

39 to compare against pre-existing quantitative assay systems.

40 n APC-dependent and APC-independent in vitro assay systems.

41 nerated from these important and informative assay systems.

42 s of data obtained from bicistronic reporter assay systems.

43 emical and genetic perturbations in cellular assay systems.

44 ling pathway using both in vitro and in vivo assay systems.

45 uncoating, as revealed with three different assay systems.

46 s 5-HT2A and 5-HT2B in relevant tissue-based assay systems.

47 and incorporating enzyme mimetic activity in assay systems.

48 nificantly reduced enzymatic activity in all assay systems.

49 oV-2 RBD and the spike antigens in different assay systems.

50 tions has been accomplished via microfluidic assay systems.

51 In cloned receptor assay systems, 12 displays at least 95-fold selectivity

52 To assess the accuracy and robustness of the assay system, 270 coded plasma specimens derived from ni

53 We used the beta-arrestin PathHunter assay system, a newly developed, generic GPCR assay form

54 This assay system accurately determined the level of spiked 3

55 This more physiologic assay system allowed the investigators to rigorously def

56 This assay system allows replication to be studied at extreme

57 Using a rapid in vivo assay system and a high-throughput mega-shift assay, we

58 ry and oxidative stress markers by multiplex assay system and ELISA assay, respectively.

59 ne were assayed in a subgenomic HCV replicon assay system and found to be potent and selective inhibi

60 are strong activators of Nrf2 in a reporter assay system and in primary human CLL based on increased

61 profile, three-dimensional (MP3D) mechanical assay system, and compared responses to those elicited b

62 The new assay system, and our findings based on it, will enable

63 emplates for data generated by dual reporter assay systems, and an online tutorial are available at o

64 nical range, diagnostic criteria, diagnostic assay systems, and treatment options for this group of d

65 DEPFMU-based assay systems are 10-100 times more sensitive for OPH an

66 is the ligand for Ly-49D in a reporter gene assay system as well as in NK cell killing assays.

67 cted for MDH or other dehydrogenases in this assay system, at physiological mole fractions of the mat

68 f reliable systems prompted us to develop an assay system based on dual enzymatic activities.

69 We describe a novel in vivo assay system based on hepatocyte transplantation that pe

70 Using an assay system based on partially differentiated embryonic

71 SpoVT we utilized an in vivo multimerization assay system based upon fusion of the domains to the DNA

72 This is particularly true in modern assay systems based on DNA monolayers at gold electrode

73 Assay systems based on this technology were developed to

74 Using SUMO fusions, a novel assay system, based on poliovirus RNA-dependent RNA poly

75 nucleases can be preliminarily tested in our assay system before their downstream application in plan

76 In a Gal4-LUC assay system, blockade of the PI3K pathway significantly

77 In liquid assay system, both GO and MGO inhibited the target bacte

78 only provide a novel plant pre-mRNA cleavage assay system, but also suggest a cross-kingdom functiona

79 (MAMEF), we show that a simple protein-based assay system can be optically amplified approximately 10

80 he potency and safety profiles and the CYP51 assay system can be used in structure-activity studies i

81 hich we have named EViTAS (for ex vivo tumor assay system), can recapitulate salient aspects of tumor

82 MG) assay for inorganic phosphate to form an assay system capable of determining inorganic phosphate

83 Four of the assay systems caused false-positive results for at least

84 In this assay system, changes in the emission spectrum of the fl

85 ing PCR and a semi-high-throughput, scalable assay system confirmed the initial discovery results in

86 Our previous study using an in vitro assay system demonstrated that Fgf and Gdf11 signals loc

87 Characterization of this assay system demonstrates that the FITC-labeled dextran

88 This sensitive DNA repair assay system demonstrates the complexity of response to

89 We have developed a DSB repair assay system, designated CDDR (CRISPR-Cas9-based Dual-fl

90 iates of DSB repair by using a budding-yeast assay system designed to mimic physiological DSB repair.

91 tomation of complex primary human cell-based assay systems designed to capture emergent properties ca

92 In an in vitro NHEJ assay system, DNA end joining was reduced by NF90/NF45 i

93 We developed a reporter assay system employing a self-inactivating retrovirus an

94 The assay system exhibits an intrinsic dynamic range of two

95 The reported assay system faithfully replicates the pharmacology of t

96 These findings provide a simple assay system for cardiac induction that may allow elucid

97 on, and high throughput make it an effective assay system for clinical laboratory diagnosis of HCV an

98 ned this hypothesis using a highly sensitive assay system for detection of misfolded protein seeds in

99 ia rickettsii were determined using a plaque assay system for enumeration and isolation of mutants.

100 yme donor immunoassay (CEDIA) and a Beta-Glo assay system for generation of bioluminescent signal.

101 demonstrated using a transient transfection assay system for GR transactivation.

102 h reporter provides a novel, high-throughput assay system for identifying compounds that bind to and

103 ivate 5-HT2B receptors, thus validating this assay system for its ability to predict medications that

104 nt of a 96-well plate-based purification and assay system for measuring chlorophyllase activity.

105 rudin folding is utilized here to develop an assay system for measuring the activity of disulfide oxi

106 his paper describes an in vitro fluorometric assay system for protein splicing based on the RecA inte

107 ong with gene fusion approaches, a sensitive assay system for signal sequences that utilize the SRP p

108 We previously constructed an assay system for studying enterobacterial beta-lactam re

109 We have established a quantitative assay system for the analysis of cap interaction with PB

110 Efforts to develop a standardized 2-tier assay system for the detection of antiretinal antibodies

111 This integrated assay system for the detection of HIV-1 drug resistance

112 A novel dot blot-based assay system for the detection of IgE against alpha-Gal

113 r the zebrafish pronephros can be used as an assay system for the development of glomerular function

114 As such, this will be a valuable assay system for the dissection of MITE transposition an

115 ur characterization of an in vitro signaling assay system for the mechanism of Tax-mediated activatio

116 To establish a simple and rapid assay system for the monitoring of R5 HIV-1 replication

117 , we describe the development of an in vitro assay system for the rapid detection and quantification

118 We propose the design of a simple assay system for the search of other chaperones that beh

119 its meiotic spindle will provide a sensitive assay system for the study of reproductive toxins.

120 method should provide a fluorescent reporter assay system for the wide variety of beta-gal systems cu

121 une response, and the sensitivity of various assay systems for detecting WNV in blood.

122 nt reactions that occur in commonly employed assay systems for the quantification of PPO in fruits an

123 sults from phenotypic screenings in cellular assay systems for viral replication.

124 newly developed mitochondrial protein import assay system from Trypanosoma brucei to demonstrate that

125 In a human whole-blood assay system, GBS type III COH-1 potently induced substa

126 In agarised assay system, GO and MGO exhibited antimicrobial activit

127 Finally, in our assay systems, H(2)O(2)-induced cell death was not due t

128 light, a sensitive antibody (Ab) microarray assay system has been developed for specific detection o

129 This assay system has the potential for simultaneous screenin

130 We believe this new assay system has the potential to be a low-cost method f

131 Bicistronic reporter assay systems have become a mainstay of molecular biolog

132 Two homogeneous assay systems have been combined to provide a new cell-b

133 We now show that, using the DASL assay system, highly reproducible tissue- and cancer-spe

134 oblasts to pluripotency, indicating that the assay system identifies functional Oct4 cofactors.

135 used the Drosophila ventral epidermis as our assay system in a series of genetic screens to identify

136 undertaken to determine the accuracy of this assay system in detecting resistance-associated mutation

137 imeras and Gal4-Luc reporter co-transfection assay system in HepG2 cells.

138 previously determined using a tissue culture assay system in human cells (HeLa).

139 abidopsis plant as well as the SEAP reporter assay system in MCF-7 breast cancer cell-line; the anti-

140 The lack of such an assay system in plants hampered the study of plant mRNA

141 We have developed an assay system in Saccharomyces cerevisiae to study recipr

142 After evaluating the assay system in the hybrid paper-SPCE cell, the three-el

143 as evaluated utilising the pER8:GUS reporter assay system in transgenic Arabidopsis plant as well as

144 was confirmed in a cell-based mineralization assay system in vitro.

145 We used an assay system in which hundreds of meiotic spindles can b

146 initially evaluated using an in vitro shear assay system in which interactions between circulating c

147 r fluorescence recovery after photobleaching assay system in which two H1 proteins fused to spectrall

148 on using different transduced cell lines and assay systems in the absence of an independent thymidine

149 and characterized a plant in vitro cleavage assay system, in which nuclear protein extracts from Ara

150 This assay system includes a semiautomated procedure, using 9

151 his paper describes a microfabricated enzyme assay system including a micromixer that can be used to

152 in a variety of cell-free and in vitro redox assay systems, including oxidant production by transitio

153 This assay system is able to detect enzymatic activity at enz

154 data analysis, and the modular design of the assay system is amenable to straightforward adaptation f

155 This assay system is capable of detecting and differentiating

156 The assay system is comprised of a plastic microfluidic cart

157 The assay system is easy to use, portable, and stable for at

158 ection, consensus agreed that a standardized assay system is needed to detect serum antiretinal antib

159 A major merit of the proposed assay system is the potential to accommodate the analysi

160 We believe that this assay system is uniquely capable of advancing our unders

161 termed the multiple-enzyme/multiple-reagent assay system (MEMRAS), relies on the use of reagents suc

162 ed, as a read-out, a single kind of cellular assay system: neuroblastoma cells that are persistently

163 We have developed an assay system of budded supported membrane tubes displayi

164 se DNA contamination was detected in the PCR assay system or the clinical samples.

165 In a reporter assay system, ORF30 and ORF34 were required for MHV-68 t

166 members of the RsbR coantagonist family and assayed system output using a reporter fusion.

167 In an in vitro biofilm assay system, P. aeruginosa displayed strain-specific bi

168 This assay/system performed well in clinical testing of regul

169 e development of more reliable and sensitive assay systems, possibly formatted for cytochemical appli

170 Difficulties with previous assay systems prompted us to develop retroviral vectors

171 The combined purification and assay system provides a convenient and rapid method for

172 this SNP linkage panel with the multiplexed assay system provides a previously unattainable level of

173 Thus, the assay system provides a simple yet highly sensitive plat

174 SUMOylation in both whole-cell and cell-free assay systems, raising the possibility that the deacetyl

175 We show that our in vitro assay system recapitulates the effect of PS1 mutations o

176 vailable RAF inhibitors performed using this assay system revealed that the inhibitors exhibit variou

177 target to pursue for further high throughput assay system screening.

178 We have designed a quantitative assay system sensitive enough to detect differences betw

179 The DASL assay system should prove useful for high-throughput exp

180 und 1 and 2 was tested in different in vitro assay systems such as free radical scavenging assay, 3-(

181 tration of its substrates in a mixed micelle assay system, suggesting that catalysis occurs at the me

182 question, a new cell-based trans-activation assay system suitable for ZIC proteins in HEK293T cells

183 ductase and PKC zeta, transphosphorylated in assay systems supportive of activity of only one of the

184 Two different assay systems, surface plasmon resonance and a microtite

185 Here, we have developed an integrated assay system that allows simultaneous examination of dou

186 n fluorescence protein (EGFP)-based reporter assay system that allows us to perform efficient and hig

187 Therefore, we were looking for an assay system that can be used for heme concentration det

188 dy was to develop an alternative, label-free assay system that can reliably measure NEM and PSA in pa

189 nt of a high-throughput circadian functional assay system that consists of luminescent reporter cells

190 cal end points in dynamic networks and as an assay system that could be used to identify electric phe

191 ped a Ni(2+)-nitrilotriacetic acid liposomal assay system that efficiently conjugates histidine-tagge

192 scribe an Escherichia coli-based integration assay system that has allowed us to characterize attN1 i

193 therefore developed a low-phosphate, low-pH assay system that is more realistic with respect to soil

194 alpha activity, determined using an in vitro assay system that lacked lipids, were found to be a nonl

195 c phenotype were characterized using a novel assay system that measures fibroblast behavior in respon

196 This protocol describes an assay system that permits examination of synaptogenic ac

197 s to be screened, it is important to develop assay systems that are not only sensitive but also homog

198 In the search for such molecules, assay systems that can be adapted to high-throughput scr

199 eloped a suite of whole-blood, syringe-based assay systems that can be used to reproducibly assess in

200 relied on cell-free or accessory cell-based assay systems that do not accurately reflect in vivo res

201 d we demonstrate by the use of two different assay systems that Kap114p inhibits Nap1p-mediated chrom

202 ted, partly due to lack of reliable in vitro assay systems that reflect the in vivo functionality of

203 ves associated with frequently used NAD/NADH assay systems that rely on detection of NADH using UV ab

204 Using this assay system the dislocation of MHCI heavy chains was fo

205 Using retinyl palmitate in a micellar assay system the enzyme was active over a broad pH range

206 In a mixed micelle assay system, the apparent Km for the precursor lipid IV

207 ve developed a widely applicable recruitment assay system through which we find that BAF opposes PRC

208 We designed an assay system to allow titration of the redox state of th

209 This study established a robust assay system to analyze the function of BRCA1 in regulat

210 In this study, we developed a binding assay system to characterize EAAT ligands for all EAAT s

211 n the present study, we used the Gal4 fusion assay system to characterize the mechanism of transactiv

212 We have established a fully defined in vitro assay system to delineate mechanisms for Pol II transit

213 Furthermore, we established an assay system to detect ISGylated target proteins by cotr

214 tro and cellular activity, a high-throughput assay system to detect the attachment of [(3)H]geranylge

215 llifera (AmTRP-R) in a heterologous reporter assay system to determine the activities of various liga

216 feasibility of using the cell line CAD as an assay system to dissect the signaling pathways triggered

217 They use this cell-based assay system to help explain the clinical manifestations

218 novel co-culture cell-based high throughput assay system to identify compounds that could potentiall

219 ure library (FOREST), a multiplexed affinity assay system to identify functional interactions from tr

220 Previously, we utilized a unique assay system to identify mutations that increased minisa

221 The lens provides an assay system to identify pathways critical for fiber cel

222 eloped malachite green-based high throughput assay system to identify SerB2 inhibitors.

223 , we exploit a sensitized human primary cell assay system to investigate the biological function of d

224 Here we report an in vitro assay system to measure synthesis of both mtDNA and 7S D

225 We established a cell-based LIPG enzymatic assay system to measure the inhibitory effect of XEN445

226 ssays were developed on a robust multiplexed assay system to provide a combination of very high accur

227 activity were investigated using an in vitro assay system to provide further insight into the mechani

228 to examine the performance of the FAST-DOSE assay system to quantify intracellular protein changes i

229 nd demonstrate the feasibility of using this assay system to rapidly screen for compounds that modula

230 ier report, we optimized a microtiter format assay system to screen potential bioactive compounds usi

231 igase was used in a ligation-based detection assay system to show increased sensitivity over T4 DNA l

232 Second, we employ this assay system to show that a dualsteric design principle,

233 We used a solid-phase kinase assay system to show that PKR does not transfer its own

234 st, we used a human PBMC-based proliferation assay system to simultaneously monitor the effect of TLR

235 The availability of a transplantation assay system to unequivocally identify male germline ste

236 We used cell-based assay systems to investigate the mechanisms responsible

237 w such ROS function, however, limitations of assay systems to measure ROS need to be appreciated, and

238 t BMP signaling, we developed novel in vitro assay systems to monitor trans signaling using Drosophil

239 We use two assay systems to show that cellular PTEN phosphatase act

240 ents derived from cells and other biological assay systems treated with small molecules.

241 eliable cell-based high-throughput screening assay system using an HCV replicon model to identify sma

242 /activin subfamily, we developed a cell-free assay system using soluble receptor-Fc fusion proteins.

243 We have developed a novel assay system using Streptolysin-O permeabilized neutroph

244 We developed a live-cell high-throughput assay system using the baker's yeast Saccharomyces cerev

245 rthermore we report a simple "membrane-free" assay system using the V0 proton channel-specific inhibi

246 Assay systems using the fluorogenic aldehyde were valida

247 We developed two assay systems (using human dendritic cells and Aedes aeg

248 The assay systems utilize three proprietary technologies: (i

249 vein endothelial cells (HUVEC) with a novel assay system utilizing HUVEC immobilized on microcarrier

250 Importantly, our cuvette-based SPR assay system was also suitable for the monitoring of lig

251 The multiplexity of the assay system was demonstrated by the simultaneous detect

252 This assay system was designed to specifically detect framesh

253 A refined assay system was developed to establish that Saccharomyc

254 Ts to HIV mutagenesis, a new high-throughput assay system was developed.

255 The reported assay system was exclusively formed by nucleic acid olig

256 The limit of detection (LOD) of CT in the assay system was found to be 10 fg/mL which is equivalen

257 The assay system was found to be amenable to sensitive kinet

258 The Dual Glo Luciferase Assay System was used to determine reporter activities.

259 This assay system was used to investigate the kinetics of tra

260 A model FRET-based caspase-3 assay system was used to test the performance of the que

261 The assay system was validated by screening potential cataly

262 with the help of a novel cell-based reporter assay system we characterized the first RhCMV encoded Ig

263 However, using an in vitro assay system we demonstrate that H. pylori WaaA is a bif

264 Using this assay system, we analyzed the promoter specificity of th

265 As an application of this assay system, we characterized the association of transc

266 beta(3) subunits, in a transient expression assay system, we compared the efficiencies of infection

267 With this assay system, we demonstrate that the replication capaci

268 Using transfected cells as an in vitro assay system, we demonstrate that truncated syntaxins la

269 By using an in vitro assay system, we demonstrate the presence of lipid A 1-p

270 Previously, using a chromosomal reversion assay system, we established that an adaptive mutagenic

271 Utilizing this assay system, we find that gene conversion events at the

272 Using an in vitro assay system, we found that expression of wild type huma

273 Surprisingly, using a recently developed assay system, we found that these factors are not direct

274 RXR-VDR mammalian two-hybrid (M2H) biologic assay system, we measured vitamin D metabolite uptake an

275 on ubiquitination of p27 in a reconstitution assay system, we now report a noncatalytic requirement f

276 ng this three-channel terbium-based, TR-FRET assay system, we show in one experiment that the additio

277 Using a transformation-based assay system, we show that most of the apparent BIR even

278 h mass spectrometry analysis and an in vitro assay system, we showed that the loss of LpxEHP activity

279 ing a tetracycline-regulated gamma-GCSh mRNA assay system, we systematically dissected the cis-acting

280 Using different assay systems, we present evidence that ADAMTS13 binds t

281 sing immunoblot-based as well as single-cell assay systems, we present evidence that Drosophila glypi

282 Using chicken embryonic endoderm as an assaying system, we found that ectopic Mfng expression i

283 As such, several in vivo assay systems were developed to evaluate gene editing ca

284 ations were defined in pilot rat studies and assay systems were used with xanthine oxidase and activa

285 umi-Gal 530), and a bioluminescent (Beta-Glo Assay System), were employed in the study.

286 ture of Cyt b was obtained using a cell-free assay system where p47SH3AB enhanced superoxide producti

287 In this work, we developed a novel BiLC assay system which is sensitive and specific to quantify

288 To this end, we developed a new assay system which supports very high degrees of multipl

289 llectively, these findings suggest that this assay system, which we have named EViTAS (for ex vivo tu

290 However, assay systems, which enable the straightforward discover

291 esis, two separate three-dimensional culture assay systems, which model chick atrioventricular cushio

292 Our cell-free binding assay system will allow for testing of models of recepto

293 This sensitive assay system will become a powerful tool to discover nex

294 It is anticipated that this high-throughput assay system will greatly facilitate nucleotidyltransfer

295 oduction of ROS was measured using a coupled assay system with Amplex Red.

296 hibits utility as an automated multiplexable assay system with applications to high-content chemical

297 Utilizing a model sandwich assay system with biotinylated anti-IgG as the capture a

298 Our results suggest that this novel assay system with this new TDP2 substrate can be used fo

299 ated potent enzyme activity in a chromogenic assay system, with select examples also demonstrating po

300 mutants in the crystal violet-based biofilm assay system yielded six mutants that exhibited greatly