ライフサイエンスコーパス: auxotroph

1 dat, can support slow growth of an L-alanine auxotroph.

2 nd met31 met32 double mutants are methionine auxotroph.

3 lds a strain that is a stringent lyso-PtdEtn auxotroph.

4 unlike many microorganisms, was a glutamate auxotroph.

5 desaturate exogenous stearate and was an UFA auxotroph.

6 Chlamydia pneumoniae is a natural tryptophan auxotroph.

7 defunct glycolytic pathway of the bacterial auxotroph.

8 ed from a normally avirulent p-aminobenzoate auxotroph.

9 viously to support the growth of an inositol auxotroph.

10 through expression in an E. coli methionine auxotroph.

11 ds, but the lysC strain is not an amino acid auxotroph.

12 ior of Caenorhabditis elegans, a cholesterol auxotroph.

13 of SHAM-sensitive respiration of the E. coli auxotroph.

14 ied by complementation of a bacterial purine auxotroph.

15 ontain an insertion in ura3 and was a uracil auxotroph.

16 ngs demonstrate that P. carinii is an AdoMet auxotroph.

17 ional complementation of an Escherichia coli auxotroph.

18 , and shown to support growth of a cobalamin auxotroph.

19 One mutant, JJ104, was a leaky acetate auxotroph.

20 demethylation of lanosterol and is itself an auxotroph.

21 A concentration than the ancestral synthetic auxotroph.

22 y to study the physiology of a nascent B(12) auxotroph.

23 roven by the reduced virulence of a cysteine auxotroph.

24 plsX double knockout strain was a fatty acid auxotroph.

25 pdxR mutant also exhibited as a vitamin B(6) auxotroph.

26 es for C. trachomatis, which is a tryptophan auxotroph.

27 owth arrest, an unusual phenotype for an Mtb auxotroph.

28 tation, suggesting that blk1-R is a thiamine auxotroph.

29 orting the growth of S. cerevisiae polyamine auxotrophs.

30 ssue by using Salmonella typhimurium leu-arg auxotrophs.

31 icted media, confirming them as his-negative auxotrophs.

32 absence of large collections of informative auxotrophs.

33 resulted in growth inhibition of lyso-PtdEtn auxotrophs.

34 ic mutants, including amino acid and vitamin auxotrophs.

35 than half of the algal kingdom are cobalamin auxotrophs.

36 tryptophan (7AW), using Escherichia coli Trp auxotrophs.

37 t the growth of the tryptophan and histidine auxotrophs.

38 ire1Delta and hac1Delta mutants are inositol auxotrophs.

39 pe3Delta, and spe4Delta are all beta-alanine auxotrophs.

40 h genes, however, are unsaturated fatty acid auxotrophs.

41 age pathways of Leishmania, which are purine auxotrophs.

42 onal complementation of Escherichia coli His auxotrophs.

43 cleave SREBPs at Site-2 and are cholesterol auxotrophs.

44 mutants; these strains are therefore glucose auxotrophs.

45 oteins, which rescued four different E. coli auxotrophs.

46 bA2(Ts) or fabB15(Ts) unsaturated fatty acid auxotrophs.

47 a useful method for screening for polyamine auxotrophs.

48 r vaccine strategies using engineered uracil auxotrophs.

49 Chlamydia and Leishmania, natural tryptophan auxotrophs.

50 an adequate purine source for gastric purine auxotrophs.

51 f triple-negative samples that are glutamine auxotrophs.

52 depend on oxidative metabolism but are heme auxotrophs.

53 enterica yhhK strains are also pantothenate auxotrophs.

54 As predicted, DeltagcvH strains are lipoate auxotrophs.

55 Met31p and Met32p respond poorly, like other auxotrophs.

56 ncorporated into proteins using L-methionine auxotrophs.

57 (iii) In derepressed auxotrophs, 100% of the codons containing the mutable ba

58 pha-ribazole, which is used by another B(12) auxotroph, a Roseovarius sp., to produce the corrin ring

59 , hac1Delta and ire1Delta, are also inositol auxotrophs, a phenotype associated with defects in expre

60 We generated a diaminopimelic acid (DAP) auxotroph (AA400) of L. pneumophila by disruption of the

61 athogen Listeria monocytogenes, a riboflavin auxotroph, acquires flavins during infection.

62 no longer be detected in mice receiving the auxotrophs after 16-32 weeks, and that infected SCID mic

63 We identify 54 auxotrophs, along with the corresponding metabolic and g

64 The citB2 mutant was a glutamate auxotroph and accumulated citrate, while the citB7 mutan

65 l complementation of an Escherichia coli Tyr auxotroph and by enzyme assays.

66 ince Enterococcus faecalis is a natural heme auxotroph and cause of bloodstream infection, we explore

67 ion, both mutants complement an E. coli OSBS auxotroph and have measurable levels of OSBS activity.

68 ound that an ade13Delta mutant is an adenine auxotroph and is unable to successfully cause infections

69 a nicotinamide adenine dinucleotide (NAD(+)) auxotroph and its growth depends on the environmental su

70 an parasite Leishmania amazonensis is a heme auxotroph and must acquire this essential factor from th

71 L. monocytogenes is a lipoate auxotroph and must scavenge this critical cofactor, usin

72 iencies-were co-cultured with an L-histidine auxotroph and optimized via adaptive laboratory evolutio

73 Caenorhabditis elegans is a heme auxotroph and relies solely on environmental heme for su

74 d the resulting strain was both a fatty acid auxotroph and required de novo fatty acid synthesis for

75 thesis, which converts Mtb into a tryptophan auxotroph and restores the efficacy of a failed host def

76 sis challenge can be achieved with a leucine auxotroph and suggest that to induce optimal protection,

77 A BR2.024 dapB mutant is a diaminopimelate auxotroph and tabtoxin negative.

78 A Schu S4 DeltapanG strain is a pantothenate auxotroph and was genetically and chemically complemente

79 w, on average, 83% overlap between unrelated auxotrophs and 35% with previously published transcripto

80 ly, C. trachomatis and C. pneumoniae are Trp auxotrophs and are starved for this essential nutrient w

81 for our studies because they are cholesterol auxotrophs and can be depleted of cholesterol by growth

82 paired mitochondrial function become uridine auxotrophs and can be maintained with high micromolar co

83 vaccine by constructing a lineage of guanine auxotrophs and conducted a double-blind, placebo-control

84 rnate host for alphaviruses, are cholesterol auxotrophs and contain very low levels of this sterol.

85 which the promoter is highly methylated are auxotrophs and express ASY at very low levels.

86 These mutants are choline auxotrophs and fall into two unlinked complementation gr

87 screen in Mycobacterium smegmatis for biotin auxotrophs and identified pyruvate carboxylase (Pyc) as

88 e ease with which we were able to screen for auxotrophs and mutants with pigmentation and capsular ph

89 the prevailing laboratory practice of using auxotrophs and nutrient supplemented media, these result

90 p1 mutants consist of two groups, tryptophan auxotrophs and prototrophs, that differ significantly in

91 firmed that zwf1Delta mutants are methionine auxotrophs and report that they also are oxygen-sensitiv

92 susceptible BALB/c mice were immunized with auxotrophs and subsequently challenged with virulent Myc

93 the conclusion that AM fungi are fatty acid auxotrophs and that plants provide their fungal endosymb

94 The resulting strain, CSDX1, was a glutamate auxotroph, and enzyme assays confirmed the absence of a

95 C. glabrata is a NAD(+) auxotroph, and its growth depends on the availability of

96 lciparum (PfADA) is essential in this purine auxotroph, and its inhibition is expected to have therap

97 C. glabrata is an NA auxotroph, and NA-induced EPA expression is likely the r

98 e ino1Delta/ino1Delta mutant was an inositol auxotroph, and the itr1Delta/itr1Delta mutant was unable

99 de conditional lethal mutations, conditional auxotrophs, and conditional mutants of the eut (ethanola

100 are viable, are not threonine or methionine auxotrophs, and express wild-type levels of aspartokinas

101 These strains are uracil auxotrophs, and their growth was attenuated on cysteine

102 t to mycorrhizal fungi, which are fatty acid auxotrophs, and this lipid export requires the glycerol-

103 Here we show that T. gondii uracil auxotrophs are completely avirulent not only in immune-c

104 A few of the correlated thiamine auxotrophs are predicted to produce pantothenate, which

105 We have characterized one of these auxotrophs as being disrupted in cysA.

106 encode a BioZ active site mutant are biotin auxotrophs, as are strains defective in CaiB which catal

107 The data for all auxotrophs at all GRs recapitulated the features of aero

108 growth, and by limiting its availability to auxotrophs, B12-producing organisms may facilitate coord

109 Here, we present a Lactococcus lactis Trp auxotroph-based expression system for efficient incorpor

110 The sucA mutant is not a succinate auxotroph but has a reduced ability to use glutamate as

111 ccumulate in tryptophan, adenine, and uracil auxotrophs but not in prototrophic strains, indicating t

112 ions are induced during starvation of a hemA auxotroph, but the level of induction observed was maxim

113 aD) mutant was confirmed to be a strict B(6) auxotroph, but the pdxT (yaaE) mutant turned out to be a

114 dicated that gdhA mutants were not glutamate auxotrophs, but attempts to isolate similar mutants with

115 n that E. coli yhhK strains are pantothenate auxotrophs, but the role of YhhK in pantothenate biosynt

116 rst, growth complementation of an L-arginine auxotroph by D-arginine was abolished by a lesion at dau

117 Here we show that two bacterial B(12) auxotrophs can salvage different B(12) building blocks a

118 owth and metabolite release in an amino-acid auxotroph community demonstrates that the interaction ne

119 Subcutaneous immunization with this auxotroph conferred protection in C57BL/6J mice against

120 smegmatis afforded a cysteine and methionine auxotroph consistent with a metabolic branch point cente

121 strain were isolated that were octanoic acid auxotrophs consistent with biochemical studies indicatin

122 iously shown that a P. aeruginosa tryptophan auxotroph could grow on tryptophan-depleted medium with

123 nation with the nonreplicating type I uracil auxotroph cps1-1 strain.

124 able to complement their respective E. coli auxotrophs, demonstrating that they encoded functional e

125 We constructed synthetic auxotrophs dependent on sAAs that were not rescued by cr

126 (yaaE) mutant turned out to be a conditional auxotroph depending on the availability of ammonium in t

127 The survival rates of nongrowing auxotrophs deprived of uracil or leucine depended on the

128 I results in an unsaturated fatty acid (UFA) auxotroph despite the presence of fabK, a gene encoding

129 This apparently simple auxotroph did not grow well in rich media containing exc

130 gle-amino-acid starvation, whereas a proline auxotroph did.

131 Tryptophan and histidine auxotrophs did not survive single-amino-acid starvation,

132 rates of two isogenic Escherichia coli K-12 auxotrophs differing only in relA have been determined i

133 meshifting was also prevalent in a polyamine auxotroph double mutant, a polyamine-independent regulat

134 tant strain was also found to be an inositol auxotroph due at least in part to an inability to proper

135 wth of E. coli strain BB26-36 which is a G3P auxotroph due to an altered G3P acyltransferase activity

136 epatocellular carcinoma (HCC) is an arginine auxotroph due to argininosuccinate synthetase I deficien

137 -/-) mice with an avirulent T. gondii uracil auxotroph elicited robust IFN-gamma responses and protec

138 Since humans are ascorbate auxotrophs, enhancing the nutritional quality of a widel

139 we demonstrate that treatment of the sterol auxotrophs erg25 slu1 or erg25 slu2 with azole antibioti

140 An ergosterol auxotroph, erg25, which fails to demethylate and concomi

141 ituted protein was induced in the methionine auxotroph, Escherichia coli DL41DE3, purified, and cryst

142 idase, and that the bab2_0247 mutant is a RF auxotroph exhibiting a lower level of intracellular infe

143 t signaling by growing, in chemostats, yeast auxotrophs for histidine, lysine, or uracil in excess of

144 Mutants lacking both of these enzymes were auxotrophs for isoleucine, whereas single mutants were c

145 Cells of the immune system are auxotrophs for most amino acids, including several nones

146 Many algae are auxotrophs for vitamin B(12) (cobalamin), which they nee

147 With the exception of heme auxotrophs, gentamicin-resistant SCVs displayed greater

148 medium-copy levels of abgAB, p-aminobenzoate auxotrophs grew on 50 nM p-aminobenzoyl-glutamate.

149 Two additional auxotrophs grew poorly in the absence of acetate but con

150 Six of the auxotrophs had an absolute growth requirement for acetat

151 In these sulfur auxotrophs, heavy sulfate is channeled exclusively into

152 gen, Entamoeba histolytica, is a nucleo-base auxotroph (i.e. lacks the ability to synthesize purines

153 er trypanosomatid parasites, they are purine auxotrophs (i.e. lack the ability to synthesize purines

154 genomic library in an E. coli DeltadapD DAP auxotroph identified ct390 as encoding an enzyme that re

155 ave a similar phenotype to the bio1 and bio2 auxotrophs identified using forward genetic screens for

156 test of the vaccine potential of the leucine auxotroph, immunocompetent BALB/c mice, susceptible to f

157 d by expressing the proteins in a methionine auxotroph in the presence of l-methionine with the side

158 the limited collection of well-characterized auxotrophs in flowering plants.

159 Growth of the auxotrophs in medium containing [(14)C]acetate yielded (

160 Psd1p that relies on isolating ethanolamine auxotrophs in suitable (psd2Delta) genetic backgrounds.

161 rocedure was developed for the enrichment of auxotrophs in the antibiotic-insensitive archaebacterium

162 Their results were obtained with a leucine auxotroph; in leucine prototrophs grown in a medium lack

163 ruption of ask was also possible in a lysine auxotroph incapable of converting DAP to lysine.

164 t of therapeutic agents in humans and purine auxotrophs, including malarial parasites.

165 Several classes of methionine auxotrophs, including metX and metW mutants, exhibit red

166 purine base salvage in humans and in purine auxotrophs, including Plasmodium falciparum, the leading

167 Strains lacking FabB are UFA auxotrophs indicating that the enzyme catalyzes an essen

168 Exploiting the ability of the inositol auxotroph, ino1, to use exogenous GroPIns as an inositol

169 A single injection of the uracil auxotroph into BALB/c mice induces long-term protective

170 The deltaleuD deltapanCD double auxotroph is fully attenuated in the SCID mouse model an

171 criptional growth-rate response (GRR) in the auxotrophs is only 40-50% of the magnitude in prototroph

172 A second acetate auxotroph, JJ117, had an absolute growth requirement for

173 Members of the Nematoda are heme auxotrophs, lacking the ability to synthesize heme; howe

174 ases to the growth of the 14-amino-acid (aa) auxotroph Lactobacillus helveticus CNRZ32, single- and m

175 Similar comparisons using leucine or uracil auxotrophs limited on leucine or uracil again showed pat

176 sed for S1P because all previous cholesterol auxotrophs manifest defects in S2P, which is encoded by

177 f E. faecalis in mice; the attenuated purine auxotroph may provide a system for developing vectors fo

178 osomatids relevant to human health, are heme auxotrophs, meaning they must import it from their mamma

179 Finally, using the Drosophila sterol auxotroph model, we demonstrate the unique ability of ex

180 unencapsulated, and para-amino benzoic acid auxotroph mutant TIGR4 strains to assess the effects of

181 and it pinpoints weaknesses of commonly used auxotroph mutants for investigating metabolism.

182 ion of fungal proteomes, which often require auxotroph mutants to fully metabolically label.

183 trains, such as type I nonreplicating uracil auxotroph mutants, are highly effective in eliciting lif

184 . < or =10 ng/ml), we have generated acetate auxotrophs NMBACE1 from encapsulated Neisseria meningiti

185 plementation of two C. nicotianae pyridoxine auxotrophs not mutant in PDX1.

186 eptides from the host environment, as a heme auxotroph NTHi utilizes host hemoproteins as a source of

187 ns necessary to uptake specific metabolites, auxotrophs obtain altered metabolic flux distributions,

188 i -100 mV; a respiration-deficient menadione auxotroph of 6850) were used to assess the influence of

189 er and transformed into a D-Ala-D-Ala ligase auxotroph of Escherichia coli possessing deletions of bo

190 ed by functional complementation of a purine auxotroph of Escherichia coli that also harbors deficien

191 A leucine auxotroph of M. tuberculosis was created by allelic exch

192 genicity of a double lysine and pantothenate auxotroph of Mycobacterium tuberculosis in mice.

193 In this study, we used an acetate auxotroph of Neisseria meningitidis serogroup B to facil

194 chC expression is greatly repressed in a B12 auxotroph of Rhodobacter capsulatus and that B12 regulat

195 It is known that auxotrophs of a variety of bacteria are attenuated in vi

196 e the results of a systematic screen for His auxotrophs of Arabidopsis (Arabidopsis thaliana).

197 irst step, we identified a number of E. coli auxotrophs of central metabolites that can form viable e

198 d S167C complemented the growth of thymidine auxotrophs of E. coli in medium lacking thymidine.

199 s confirmed by complementing the orthologous auxotrophs of Escherichia coli (bioD and bioA).

200 Tryptophan auxotrophs of Escherichia coli in which mutations were m

201 measured autoinducer synthesis by amino acid auxotrophs of Escherichia coli that contained luxI on a

202 Riboflavin, pantothenate, and biotin auxotrophs of Histoplasma were generated to probe whethe

203 Screening for amino acid auxotrophs of Mycobacterium bovis BCG, obtained by trans

204 Complementary auxotrophs of Saccharomyces cerevisiae amino acid and nu

205 Transformation of tryptophan auxotrophs of Streptomyces coelicolor A3(2) and subseque

206 screened a number of independent pyrimidine auxotrophs of Sulfolobus acidocaldarius for deletions an

207 Uracil auxotrophs of two H. capsulatum restriction fragment len

208 ify six pairs out of fifteen composed of six auxotrophs of Yarrowia lipolytica that spontaneously for

209 otein enabling the growth of p-aminobenzoate auxotrophs on exogenous p-aminobenzoyl-glutamate.

210 sed transpositional mutagenesis of a proline auxotroph (PAO951) to isolate an ornithine utilization (

211 Rapid death of threonine and homoserine auxotrophs points to a distinct susceptibility of Mtb to

212 as vaginalis is a parasitic protozoan purine auxotroph possessing a unique purine salvage pathway con

213 t Mycobacterium tuberculosis, several of the auxotrophs produced comparable protection against intrav

214 Two spontaneous revertants of these latter auxotrophs regained the ability to grow normally in the

215 The M. tuberculosis lysine auxotroph requires 25-fold more lysine on defined medium

216 Plasmodium falciparum is a purine auxotroph requiring hypoxanthine as a key metabolic prec

217 n mutagenesis, was used to select methionine auxotrophs requiring a sulphur-containing amino acid for

218 nd the structural requirements necessary for auxotroph rescue, we investigated the biophysical proper

219 n menD or hemB, yielding menadione and hemin auxotrophs, respectively, and studied in the rabbit endo

220 ng from Chlamydia Thus, how Chlamydia, a Trp auxotroph, responds to Trp starvation in the absence of

221 his study, we constructed similar tryptophan auxotroph revertants and found that the reversion result

222 Plasmodium falciparum is a purine auxotroph, salvaging purines from erythrocytes for synth

223 Unlike the wild-type, both pyrimidine auxotrophs secreted substantial quantities of orotate, s

224 MIs for 16 mutable bases in auxotrophs, selected during starvation in derepressed ge

225 Studies with sterol auxotrophs show that, even in the presence of sterols wh

226 use carA mutants are arginine and pyrimidine auxotrophs, single-site insertions at the glmS attTn7 si

227 nt for the growth of S. cerevisiae polyamine auxotrophs (spe1Delta, spe2Delta, and spe3Delta).

228 he live, attenuated Toxoplasma gondii uracil auxotroph strain cps1-1 induces long-lasting immunity ag

229 ing cps, an avirulent, nonreplicating uracil auxotroph strain of the parasite Toxoplasma gondii (T. g

230 The biotin auxotroph strain S288c was able to grow on media lacking

231 develop an avirulent nonreverting pyrimidine auxotroph strain.

232 in protein half-lives between prototroph and auxotroph strains.

233 n by detecting infection with the riboflavin auxotroph Streptococcus pyogenes.

234 deletion of ORF Vng1577 was not a cobalamin auxotroph, suggesting that either there is redundancy of

235 ant was constructed and found to be a lysine auxotroph, suggesting that this DapL homolog in methanoc

236 SCID mice infected with the pantothenate auxotroph survived significantly longer (250 days) than

237 All three auxotrophs survived complete starvation.

238 trate that erythrocytes from these ascorbate auxotrophs switch the preference of their glucose transp

239 h selection, using an unsaturated fatty acid auxotroph system, led to the isolation of variants with

240 lanosterol synthase mutant provided a sterol auxotroph that could be genetically complemented with th

241 e LVSDeltaguaA, an F. tularensis LVS guanine auxotroph that fails to replicate in the absence of exog

242 The nudA mutant was an auxotroph that grew slowly in liquid and on solid media

243 l complementation of an Escherichia coli Ile auxotroph that has been engineered to express ACC deamin

244 e roundworm Caenorhabditis elegans is a heme auxotroph that requires the coordinated actions of HRG-1

245 cal mutagenesis, we isolated an ethanolamine auxotroph that we designate pstA1-1.

246 egans and related helminths are natural haem auxotrophs that acquire environmental haem for incorpora

247 s not secreted by SRD-12B cells, cholesterol auxotrophs that lack S1P.

248 African trypanosomes are lipid auxotrophs that live in the bloodstream of their human a

249 C and DeltaOMPDC DeltaUP strains were uracil auxotrophs that rapidly lost their viability during pyri

250 included a diverse set of novel non-specific auxotrophs that result from inhibition of major biosynth

251 MP synthase, C. neoformans becomes a guanine auxotroph, the production of key virulence factors is co

252 Some methionine auxotrophs (those with blocks in the biosynthetic pathwa

253 ions also reduced the growth of this E. coli auxotroph to about 85% of the control cells containing w

254 ts suggest that the inability of the leucine auxotroph to grow in mice was due to its sequestration,

255 was devised with the use of the E. coli UFA auxotroph to isolate mutants of the castor delta(9)-18:0

256 e URA3 gene efficiently transformed the ura3 auxotroph to prototrophy.

257 ked the viability of an E. coli L-methionine auxotroph to the activity of the improved enzyme.

258 The ability of Mycobacterium tuberculosis auxotrophs to survive long-term starvation was measured.

259 ck carbamoyl phosphate synthetase II (uracil auxotrophs) to determine whether de novo pyrimidine bios

260 opy-number plasmid pACYC184; p-aminobenzoate auxotrophs transformed with the clone encoding abgAB dem

261 tarvation for leucine in an Escherichia coli auxotroph triggers metabolic activities that specificall

262 A new ergosterol auxotroph unable to grow on 3-ketosterols without the ad

263 An ergosterol auxotroph unable to synthesize sterol or grow without st

264 luciliae, a primitive trypanosomatid, purine auxotroph, up-expressed its unique, bi-functional, surfa

265 We measured the survival of yeast auxotrophs upon starvation for different nutrients and f

266 A live, nonreplicating avirulent uracil auxotroph vaccine strain (cps) of Toxoplasma triggers no

267 ting, nonreplicating, live attenuated uracil auxotroph vaccine strains in the type II Deltaku80 genet

268 A purine (but not a pyrimidine) auxotroph was considerably less lethal than wild type; g

269 A double auxotroph was generated that allowed for complete incorp

270 t lacking sulfate transport and a methionine auxotroph was hypersensitive to the CGL inhibitor propar

271 However, the leuD auxotroph was less effective than live BCG in reducing o

272 ng cultures of a homoserine and a methionine auxotroph was measured by mass spectrometry.

273 Growth of tryptophan (Trp-) auxotrophs was more strongly inhibited by phytosphingosi

274 ubiquinol oxidase from Escherichia coli with auxotrophs was used to characterize the hyperfine coupli

275 When a DeltasepS mutant (a cysteine auxotroph) was grown with (34)S-labeled sulfide and unla

276 fect observed in tumor cells, starving yeast auxotrophs wastes glucose as a consequence of the failur

277 Because NTHI is a heme auxotroph, we hypothesized that availability of heme fro

278 ars to be limited in proline because proline auxotrophs were defective for intramacrophage survival a

279 Twenty-four adenine auxotrophs were identified that exhibited supersensitivi

280 When these auxotrophs were inoculated i.p. into C57B6 mice bearing

281 Hybrids able to complement the auxotrophs were purified to homogeneity and assayed for

282 mutants, S. cerevisiae purine and pyrimidine auxotrophs were severely deficient in survival, consiste

283 ngineering Salmonella typhimurium amino acid auxotrophs which grow in viable as well as necrotic area

284 nutritional mutants, including nicotinamide auxotrophs, which are found frequently among cystitis is

285 C. neoformans adenine auxotrophs, which are less virulent in mammals, were als

286 utomated continuous evolution of a synthetic auxotroph while supplying a decreasing concentration of

287 We find that oncogenic EpdSCs are serine auxotrophs whose growth and self-renewal require abundan

288 Previously, we showed that a BCG leucine auxotroph with a transposon disruption of the leuD gene

289 Likewise, a spontaneous revertant of an auxotroph with reduced levels of CO dehydrogenase and wi

290 plementation analysis of an E. coli cysteine auxotroph with the pMEK34-14 recombinant plasmid contain

291 Upon transformation of these auxotrophs with CoGeLs, cells growing without supplement

292 In contrast, methionine auxotrophs with defects in the transcription factors Met

293 elta orm2Delta cells: the cells are inositol auxotrophs with impaired transcriptional regulation of g

294 The isolation of thiamine auxotrophs with mutations in at least five different gen

295 d was used to construct a variety of proline auxotrophs with mutations in the proABC locus.

296 ersion of the corresponding Escherichia coli auxotrophs with the same equipment and procedures.

297 ent with a scenario, in which interconnected auxotrophs withdraw amino acids from the cytoplasm of do

298 a portion of IJs grown on para-aminobenzoate auxotrophs, X. nematophila does not exhibit abortive col

299 It was established that a methionine auxotroph yeast strain could grow on either form of L-me

300 tionally lethal mutants of Escherichia coli (auxotrophs) yielded several de novo sequences, termed Sy