ライフサイエンスコーパス: avidin

1 s then used to selectively detect the target avidin.

2 tin conjugate followed by staining with FITC-avidin.

3 avidin compared to binding of HABA to native avidin.

4 iotin and its site-specific accessibility to avidin.

5 n streptavidin and is one residue removed in avidin.

6 er cooperative hydrogen-bonding effects than avidin.

7 on the well-known interactions of biotin and avidin.

8 ated liposomes to complex in the presence of avidin.

9 cognition of a biotinylated protein layer by avidin.

10 ed to bind within the biotin-binding site of avidin.

11 rane by biotinylation and cross-linking with avidin.

12 rines forming a major physiological role for avidin.

13 the specific interaction between biotin and avidin.

14 steine, lysine, or arginine functions of the avidin.

15 f a biotin-derivatized caged-xenon sensor to avidin.

16 tion opposite to the protein analytes, as in avidin.

17 ondrial extracts by using peroxidase-coupled avidin.

18 sequence of the homotetrameric glycoprotein avidin.

19 tivity between the anion and its position in avidin.

20 inding interaction analyses, an avidin/HABA (avidin/4'-hydroxyazobenzene-2-carboxylic acid) competiti

21 Earlier studies have demonstrated that avidin, a protein prevalent in egg-white and which has h

22 ing a pH-sensitive fluorophore conjugated to avidin-Ab tetramers, we found that CD244 crosslinking in

23 configuration is consistent with the biotin-avidin accessibility and internal salt-bridge data used

24 d to generate it, and with additional biotin-avidin accessibility data.

25 f residues throughout the KvAP channel using avidin accessibility to different-length tethered biotin

26 th (imino)biotinylated cargo proteins via an avidin adaptor with a high degree of spatial control.

27 njugate is then amenable to enrichment using avidin affinity capture.

28 and (2) affinity enrichment using monomeric avidin affinity chromatography columns.

29 agent, which can be used in conjunction with avidin affinity chromatography to purify biotinylated pe

30 pirolactone biotinylation, and enrichment by avidin affinity chromatography with mass spectrometry.

31 e was purified from M. tuberculosis H37Rv by avidin affinity chromatography, and the three major prot

32 from the catalytically defective p300 HAT by avidin affinity chromatography.

33 subsequent pulldown experiments with biotin-avidin affinity chromatography.

34 for the isolation of the labeled protein by avidin affinity precipitation, facilitating efforts to i

35 olumn, and after cyanogen bromide treatment, avidin affinity purification was used to capture Bio-DOP

36 e surface of magnetic beads through a biotin-avidin affinity reaction and magnetic separation.

37 dihydrofolate reductase (DHFR) locus, using avidin-affinity purification of biotinylated chromatin f

38 lised on a gold screen-printed electrode and avidin-alkaline phosphatase conjugate.

39 The detection was performed after adding avidin-ALP to perform avidin-biotin reaction; the signal

40 tion method where NP probe concentration and avidin analyte were unknown and both were determined.

41 The avidin and 5' biotinylated single-stranded DNA (ssDNA) p

42 ith the well-known complex formation between avidin and biotin as a model system.

43 n-ligand and protein-protein systems: biotin-avidin and biotin-streptavidin, barstar-dibarnase and Z

44 pecificity on bovine serum albumin (BSA) and avidin and detecting SEB in artificially contaminated mi

45 he dissociation constants (Kd) of the biotin-avidin and dopamine-boronic acid complexes were determin

46 The binding of the avidin and enzyme modified Au nanoparticles to the bioti

47 ng, rather than nonspecific contact, between avidin and functionalized xenon leads to the effects on

48 Studies of protein binding with biotin/avidin and ganglioside/cholera toxin systems show detect

49 We find that the interactive forces between avidin and graphene are mainly hydrophobic, along with s

50 erimental approach, the interactions between avidin and graphene for the development of a sensing pla

51 ence spectroscopy by utilizing conjugates of avidin and horseradish peroxidase in a microtiter plate

52 Avidin and its analogues have therefore been extensively

53 of DMSO on the structure and interactions of avidin and Mycobacterium tuberculosis (Mtb) CYP142A1 wer

54 n used to detect multivalent binding of both avidin and polyclonal anti-biotin.

55 ity in the hydrogen-bonding network for both avidin and streptavidin by greatly polarizing the urea o

56 ually strong reversible binding of biotin by avidin and streptavidin has been investigated by density

57 sorption to a pure DOPC bilayer, compared to avidin and streptavidin.

58 further shown by preparation of sensors for avidin and streptavidin.

59 ved beta-barrel structure similar to that of avidin and the cation-dependent mannose 6-phosphate rece

60 target due to the interaction between biotin-avidin and the enzyme converts electro-inactive alpha na

61 g from the high affinity interaction between avidin and the nucleic acids.

62 fully employed to detect surface-immobilized avidin, and a detection limit of 10 ng/mL was achieved.

63 like cations of serum albumin, streptavidin, avidin, and alcohol dehydrogenase were probed using cati

64 proteins, including human thrombin, PDGF-BB, Avidin, and His-tagged recombinant protein, were studied

65 digests of horse radish peroxidase, chicken avidin, and human immunoglobulin G, respectively.

66 antibody, NeutrAvidin and biotinylated anti-avidin, and the signal for protein binding on the membra

67 The biotin-fluorescent avidin approach is not a viable procedure for monitoring

68 rbon electrode on which a redox hydrogel and avidin are co-electrodeposited.

69 Streptavidin and avidin are used ubiquitously because of the remarkable a

70 lected from phage libraries and streptavidin/avidin as core protein were used for direct detection of

71 In contract, the intracellular delivery of avidin, as a non-covalent complex with a biotinylated Ta

72 tilising the automated system and the biotin-avidin assembly procedure.

73 pon binding of a biotin-containing sensor to avidin at 1.5 muM concentration, the free xenon T2 is re

74 and specific transducer for the detection of avidin at femtomolar concentrations in solution.

75 nd fluorescein isothiocyanate (FITC)-labeled avidin (Av-FITC) as the template.

76 binding of horseradish-peroxidase-conjugated avidin (avidin-HRP).

77 After avidin-based affinity purification and on-resin trypsini

78 For this purpose, we developed an avidin-based biotin capture surface based on a supported

79 To assess functional activity, fluorescent avidin-based degranulation assay, calcium mobilization,

80 n the form of sandwich antibodies and biotin-avidin-based gold nanoparticles.

81 In addition, avidin-based nanoparticles have been investigated as dia

82 on principles and biomedical applications of avidin-based nanoparticles in drug delivery and diagnosi

83 tic binding energy is predicted to favor the avidin-bicyclic urea complex due to the relatively large

84 In contrast, avidin binding effectively neutralized the potency of bo

85 exposed to thiolated biotin to introduce an avidin binding element on the surface of the gold beads.

86 mmunoglobulin G (Bt-IgG), which binds to the avidin binding sites distal to the surface and the F(c)

87 Using the biotin-avidin binding system, we showed that the faster respons

88 PE membranes were determined to be 33 nM for avidin binding to biotinylated lipids, 73.5 nM for chole

89 surface of a microfluidic channel by biotin-avidin binding.

90 ns onto SiO(2) microbead supports via biotin-avidin binding.

91 BSO, and BNB were quantitated by HPLC and an avidin-binding assay in CSF samples from a subset of 11

92 by ultrafiltration and was quantitated by an avidin-binding assay.

93 biotin by ultrafiltration and quantitated by avidin-binding assay.

94 rotein by ultrafiltration and quantitated by avidin-binding assay.

95 of biotin were determined initially as total avidin-binding substances (TABS) in CSF obtained by lumb

96 d agreement with a bulk determination of the avidin-biocytin binding ratio.

97 This methodology has been tested with the avidin-biocytin binding system for which the best-fit di

98 cific non-covalent interactions (e.g. strept(avidin)/biotin) or covalent bond formations (e.g. invers

99 The structures and biochemical properties of avidin, biotin and their respective analogues will also

100 bilize a biotinylated APSA using a classical avidin-biotin affinity approach.

101 oxylamine to enrich the modified peptides by avidin-biotin affinity chromatography and analyze them b

102 tinylated antibody for MBP using a classical avidin-biotin approach.

103 nates the contamination that can result from avidin-biotin based retrieval systems and simplifies dat

104 ignal was monitored in real-time based on an avidin-biotin binding between avidin coated QCM surface

105 ostmortem brain in molecular assays, we used avidin-biotin binding to couple superparamagnetic iron o

106 phospholipid were immobilized through biotin-avidin-biotin binding to the surface of a biotin-modifie

107 to simple bioconjugation techniques, such as avidin-biotin binding.

108 ubconjunctival injection was demonstrated by avidin-biotin chemistry.

109 of Microcystis spp. on a gold electrode via avidin-biotin chemistry.

110 bridging approaches the adhesion of oriented avidin-biotin complexes.

111 Avidin-biotin interaction is one of the strongest non-co

112 ific adsorption, specific adsorption via the avidin-biotin interaction, and immobilization of antibod

113 s immobilized on silver surface via specific avidin-biotin interaction.

114 d antibody for ciprofloxacin using classical avidin-biotin interactions.

115 lds, with biotinylated aptamers attached via avidin-biotin linkages, and horseradish peroxidase (HRP)

116 This was demonstrated using avidin-biotin particles as a simple bead-based bioassay

117 Specimens were processed for avidin-biotin permanent labeling, and subsets of the who

118 performed after adding avidin-ALP to perform avidin-biotin reaction; the signal was generated through

119 properties of the coupled moieties makes the avidin-biotin system a versatile platform for nanotechno

120 We speculate that many uses of avidin-biotin technology could be improved by using this

121 We detected GFP with pre-embedding avidin-biotin-peroxidase and GABA with post-embedding im

122 uple whole-cell recordings with an optimized avidin-biotin-peroxidase staining technique, we carried

123 imately 100-fold slower than the widely used avidin.biotin affinity pair.

124 avidin could then be immobilized via (strept)avidin/biotin binding.

125 he liposome formulation was combined with an avidin/biotin complex mechanism.

126 in ammonium carbonate buffer (which ensures avidin/biotin complexation) reveal that nonspecific inte

127 ed immobilization strategy (i.e., through an avidin/biotin linkage) is well-suited to immobilize a ne

128 IPAM) were conjugated to antibodies using an avidin/biotin system and deposited onto surfaces using a

129 ation as an alternative using either (strept)avidin/biotin, bispecific antibodies, or oligomers.

130 ASPA antibodies throughout the brain by the avidin/biotin-peroxidase detection method, and colocaliz

131 o alternative targeting techniques including avidin/biotin.

132 es and the protein varies and, unlike in the avidin:biotin complex, complete ordering of the protein

133 y for detection, and this is amplified by an avidin/biotinylated horseradish peroxidase complex.

134 lobin-alpha, ovalbumin, human transthyretin, avidin, bovine serum albumin, concanavalin, human serum

135 ich was linked to the biotin-AuNP through an avidin bridge.

136 attached to the detection antibodies via an avidin bridge.

137 omeric Trp that characterizes the tetrameric avidins but is lacking in shwanavidin.

138 e molecular relay was reprogrammed to detect avidin by using a different TFG.

139 nd are specifically targeted to GPI-anchored avidin-CD14 chimeric proteins expressed on the membranes

140 nin I on silanized glass slides using biotin-avidin chemistry, as well as through direct covalent att

141 me based on an avidin-biotin binding between avidin coated QCM surface and specific biotinylated LAMP

142 d to saturate the biotin binding capacity of avidin coated wells.

143 Proteins were affinity isolated onto (strept)avidin-coated beads and then photoreleased (PC-SNAG).

144 MB probes, we have developed a DNA array on avidin-coated cover slips and have improved analytical s

145 fold for Cy5 and 4-fold for Cy3, compared to avidin-coated glass substrates.

146 It consists of monomeric avidin-coated microbeads trapped in a pipette tip and ha

147 or XIIa) or biotinylated kallikrein bound to avidin-coated plates.

148 biotinylated, singly-labeled 23 bp DNAs onto avidin-coated SIF substrates.

149 tin moieties and spacers were immobilized on avidin-coated surfaces and characterized by surface plas

150 By pre-dosing with avidin, the biotin-avidin complex can be exploited to promote longer retent

151 By electron microscopy of the ATP synthase-avidin complex in negative stain and by subsequent image

152 mation and stability of the resulting biotin-avidin complex, it is useful to know the kinetics of the

153 t consistent with a simple mechanism whereby avidin complexation of BPP+ leads to encapsulation of th

154 model proteins, namely, transthyretin (TTR), avidin, concanavalin A (conA), and human serum amyloid P

155 tection system it was demonstrated that when avidin concentration increases a decrease in current and

156 Compared with the metal-free avidin conjugate, the avidin-metal complex was observed

157 Cy5-avidin conjugate-bound silver nanoparticles were prepare

158 tin groups on coat protein 8 (p8) to bind to avidin-conjugated enzymes.

159 wich immunoassay, biotinylated antibody, and avidin-conjugated GOx for the selective naked-eye detect

160 es (toxins), biotinylated detection Abs, and avidin-conjugated NP.

161 for subsequent detection using a variety of avidin conjugates.

162 n to the two probes and MALDI-MS analysis of avidin contained in the solution removed from the probe

163 Enzymes linked to streptavidin or avidin could then be immobilized via (strept)avidin/biot

164 en are linked to biotinylated enzymes via an avidin couple.

165 c-coglycolic acid-based NP platform, wherein avidin-decorated NPs can be targeted to multiple human D

166 apture mechanism is specific, such as biotin-avidin, deposition is optimal at medium flow rates with

167 s injection of a fluorescent or radiolabeled avidin derivative.

168 attached to one of two different neutralite avidin-derivatized mAbs directed against either human CD

169 On the example of biotin-avidin detection system it was demonstrated that when av

170 Avidin detection was not perturbed by Bovine Serum Album

171 pment of MHC tetramers, based on fluorescent avidins displaying biotinylated peptide-MHC complexes.

172 e by affinity interaction between biotin and avidin; electrochemical impedance measurements were perf

173 functional microstructures from crosslinked avidin enables submicrometer localization of controllabl

174 fied proteins (PTM) generally rely on biotin/avidin enrichment.

175 llected on six model glycoproteins (RNase B, avidin, fetuin, asialofetuin, transferrin, and AGP) as w

176 r dysgenic (alpha1S-null) myotubes, punctate avidin fluorescence co-localized with the XFP puncta for

177 The affinity of avidin for biotin allows biotinylated liposomes to compl

178 The high affinity of avidin for biotin has made it useful for many bioanalyti

179 Avidin from chicken egg white was nitrated using dilute

180 Avidin functional affinity electrophoresis (AFAEP) is a

181 These avidin functionalized UCNPs were adsorbed onto a cellulo

182 ite-specific biorecognition properties using avidin-functionalized multi-walled carbon nanotubes (MWC

183 that an anti-human transferrin receptor IgG3-avidin fusion protein (anti-hTfR IgG3-Av) inhibits the p

184 ons were spotted for P(4,5)BP/PtdIns(4)P and avidin/GM1 at varied degrees.

185 Here we design positively charged Avidin grafted branched Dextran nanostructures that util

186 aptamer of thrombin was immobilized onto an avidin-graphite epoxy composite (AvGEC) electrode surfac

187 tion-state, binding interaction analyses, an avidin/HABA (avidin/4'-hydroxyazobenzene-2-carboxylic ac

188 The avidin/HABA assay found the amount of available biotin a

189 These results indicate that avidin has access to a number of sites of the DHPR withi

190 Hydrogen peroxide (H2O2) is then reduced by avidin-HRP in the presence of TMB (3,3',5,5'-tetramethyl

191 of horseradish-peroxidase-conjugated avidin (avidin-HRP).

192 cal impedance spectroscopy (EIS) analysis of avidin immobilized on a graphenated polypyrrole (G-PPy)

193 of unmodified carbohydrates to the resulting avidin-immobilized lectins was monitored by BSI.

194 The avidin-imprinted polymeric layer selectively concentrate

195 The spectroscopic properties of 15 and 15-avidin in aqueous media were very similar.

196 as been applied for label-free biosensing of avidin in both the domains simultaneously.

197 dy, we have determined crystal structures of avidin in complex with 8-oxodeoxyguanosine and 8-oxodeox

198 o the alpha1S C-terminal was inaccessible to avidin in dysgenic myotubes (containing RyR1).

199 fluidic network that generates a gradient of avidin in solution and immobilizes this protein on the s

200 odel analyte of horseradish peroxidase (HRP)-avidin in the dynamic range of 0.1-3.0 mug mL(-1).

201 lar, the ligand-receptor binding (biotin and avidin in this paper) was not interfered with when ancho

202 Associated with avidin-induced NP cluster formation was an increase in t

203 ta(1-42)] oligomers incorporating the biotin-avidin interaction that has been a workhorse for screeni

204 inylated enzyme, attached through the biotin-avidin interaction to a fluorescent nanosphere.

205 n and exploitation of the ultrastrong biotin-avidin interaction.

206 e bicyclic urea are much weaker than (strept)avidin interactions due to relatively low polarization o

207 ol (PIP) with its binding protein and biotin/avidin interactions were employed for array measurements

208 ause the binding affinity between biotin and avidin is large (Ka=10(15) M(-1)), biotin could also ser

209 The immobilized gradient of avidin is then translated into gradients of biotinylated

210 t assay (ELISA)-based assays of the modified avidin, it was found that there are approximately three

211 ) = 4.4 +/- 1.9 x 10(-6) M) than immobilized avidin (K(D) < or = 10(-11) M).

212 nce energy transfer (FRET) experiments using avidin labeled with an Alexa488 fluorophore, which bound

213 for immobilization of specific DNA probe via avidin layer on the surface.

214 Binding of "functionalized" xenon to avidin leads to a change in the chemical shift of the en

215 Shwanavidin is an avidin-like protein from the marine proteobactrium Shewa

216 surface of microbubbles (MBs) via the biotin-avidin linkage.

217 (BBIR) composed of an extracellular-modified avidin linked to an intracellular T-cell signaling domai

218 In this case, avidin-linked glucose oxidase and streptavidin-linked ho

219 Sandwich-type Ru(II) subsetPSB/avidin<-->anti-CRP CRP anti-CRP<-->MB aggregates were fo

220 aggregates were formed when Ru(II) subsetPSB/avidin<-->anti-CRP was mixed with anti-CRP<-->MB conjuga

221 t cross-linking of enzymes, by decoration of avidin matrixes with biotinylated enzymes, or by cross-l

222 that a multi-arm cationic nano-construct of Avidin (mAv) with 28 sites for covalent drug conjugation

223 the surface of cerebellar granule neurons by avidin-mediated cross-linking, and inwardly rectifying p

224 ion curve was achieved between the amount of avidin-metal complex on the cell surface and the emissio

225 ed with the metal-free avidin conjugate, the avidin-metal complex was observed to display a stronger

226 It was noticed that the avidin-metal complexes bound on the cell surfaces could

227 increased with an increase of the number of avidin-metal complexes on the cell surface but the lifet

228 The avidin-metal complexes were conjugated with the biotin-s

229 dies illustrate the molecular basis by which avidin might act as a marker of DNA damage, although the

230 on limit of 8.2 x 10(-19) molar for a biotin-avidin model, 10(5) times more sensitive than that obtai

231 Biotinylated NP probes reacted with an avidin molecular target to form stable NP clusters, whic

232 Notably, the structure and function of the avidin molecule are largely preserved after its adsorpti

233 ano-Assembly (ANANAS) is a kind of soft poly avidin nanoparticle originating from the high affinity i

234 he superior anticoagulant efficacy and rapid avidin neutralizability of EP217609 compared with antico

235 molecule together with a biotin tag to allow avidin neutralization.

236 The Avidin-Nucleic-Acids-Nano-Assembly (ANANAS) is a kind of

237 sitive detection of four different proteins, avidin, O6-methylguanine DNA methyltransferase (MGMT), S

238 from surface-induced dissociation (SID); (3) avidin oligomers are best described as heterogeneous ens

239 e nanocomposite confirming the adsorption of avidin on graphene nanoplatelets as observed from the Fo

240 h further surface biotinylation and bridging avidin or NeutrAvidin.

241 ness of the membrane that forms a barrier to avidin penetration, allowing us to determine the magnitu

242 NP affinity for the avidin/peptide tag complexes was used to provide insight

243 ectin, Con A, and the biotin-binding protein avidin-peroxidase.

244 ried sequence and length were attached to an avidin platform and screened for affinity against the po

245 proteins were enriched by biotinylation and avidin precipitation and analyzed by tandem mass spectro

246 nd immediately bound by fluorochrome-labeled avidin present in the culture medium.

247 nfocal microscopy and a fluorochrome-labeled avidin probe, one can directly assess the alteration of

248 zobenzene-2-carboxylic acid interacting with avidin protein immobilized on polystyrene beads.

249 successfully delivered an Alexa 488-labeled avidin protein into human glioblastoma cells.

250 creating binding sites for streptavidin and avidin proteins in solution.

251 better selectivity than the one prepared via avidin, recognizing almost none of the tested Gram-posit

252 The avidin residues present in MWCNTs-Av/RuNPs hybrid nanoma

253 The assembly of these salts with avidin resulted in the formation of stable biohybrid cat

254 Instead, we propose that binding of BPP+ to avidin results in the quenching unit attaching to a posi

255 Furthermore, MALDI-MS analysis of the avidin retained on the two probes reveals that the limit

256 strategy is proposed whereby self-quenching avidin-rhodamine X, which has affinity for lectin on can

257 with a background summary of biotin/(strept)avidin self-assembly and the current design rules for cr

258 However, biotin/(strept)avidin self-assembly has several well-recognized drawbac

259 Biotin/(strept)avidin self-assembly is a powerful platform for nanoscal

260 Nevertheless, as in all avidins, shwanavidin also displays high thermostability

261 fluorescein isothiocyanate-conjugated (FITC) avidin staining.

262 results show that the binding affinities of avidin, streptavidin, and neutrAvidin for biotin were al

263 be of the same order of magnitude as that of avidin, streptavidin, and neutrAvidin.

264 A comparison of the binding properties of avidin, streptavidin, neutrAvidin, and antibiotin antibo

265 growth and show the ability to functionalize avidin structures with biotinylated reagents, an approac

266 g events which we demonstrate through biotin-avidin surface coupling.

267 es was demonstrated with the liposome-biotin-avidin system.

268 on from a CP excited state, and the proteins avidin, tau, BSA, and pepsin A.

269 (4) native top-down sequence analysis of the avidin tetramer is possible by CID in tandem-TIMS.

270 rations higher than 4% (v/v) destabilize the avidin tetramer toward dissociation and unfolding, via b

271 s in collision-induced dissociation (CID) of avidin tetramers into compact monomers, dimers, and trim

272 cess, the geometry of peptide-MHC display by avidin tetramers was examined, as well as the stability

273 that (1) tandem-TIMS/MS retains native-like avidin tetramers with deeply buried solvent particles; (

274 hrough strong inert linkages, such as biotin-avidin, that depolymerizing MTs exert a brief tug on the

275 By pre-dosing with avidin, the biotin-avidin complex can be exploited to pr

276 les (pyranine and crystal violet) as well as avidin through melittin induced membrane pores and defec

277 It becomes biotinylated in vivo, and allows avidin to be bound quantitatively to the purified enzyme

278 gG was chosen, by conjugating the film-bound avidin to biotin-labeled anti-rabbit IgG.

279 ed to rapidly determine whether the ratio of avidin to biotinylated NP was optimal or whether additio

280 del is proposed for the selective binding of avidin to DNA containing oxidatively damaged deoxyguanos

281 loiting the capacity of fluorochrome-labeled avidin to stain degranulating cells.

282 ication of various surface concentrations of avidin to the two probes and MALDI-MS analysis of avidin

283 of neutravidin, a deglycosylated variant of avidin, to surface-immobilized biotin.

284 nding positively charged microdomains of the avidin, two polyanions, poly(acrylic acid-co-maleic acid

285 of these, 15, was efficiently conjugated to avidin via an amide linkage.

286 periment in which the biotin-binding site of avidin was blocked with native biotin showed no such spe

287 formation of bioconjugates of nanogels with avidin was confirmed using optical fluorescence microsco

288 teractions of biotin and fluorophore-labeled avidin was constructed on SIFs and was subsequently comp

289 The protein avidin was detected down to 1 mug mL(-1) using our bioti

290 on, i.e., the grafting process of biotin and avidin was directly monitored optically displaying indiv

291 With this multi-arm design, <1 muM Avidin was needed - a concentration which has been shown

292 Here, HRP-avidin was substituted with the human adenosine deaminas

293 arge freedom of movement of the anion inside avidin, we also investigated the substrate scope of this

294 experiments confirmed that poly(guanine) and avidin were immobilized on the surface of silica NPs.

295 (mucin, soybean peroxidase, collagen IV, and avidin) were compared with those of the constituent suga

296 is effected by sequential immobilization of avidin, which binds to the biotin in the initial templat

297 gn of a long sought after monovalent form of avidin, which would be ideal for novel types of biotechn

298 2 domain of streptococcal protein G (PG) and avidin will be described.

299 es, and a range of oligonucleotides, bind to avidin with micromolar affinity.

300 to the polyacrylamide gel solution to embed avidin within the gel matrix by interaction with the ami