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1 cular disulfide could not rescue a GP64-null bacmid.
2 nt in cells transfected with the lef-11-null BACmid.
3 into the polyhedrin locus of the vAc(lef6KO) BACmid.
4 elayed in cells infected with the lef-6-null BACmid.
5 e levels seen with the repaired or wild-type bacmids.
6 lts seen with wild-type and vAcAN-KO/GUS-Res bacmids.
7 nation is approached, in which a recombinant bacmid, a shuttle vector that can be propagated in both
8 t in cells transfected with a vlf-1 knockout bacmid, aberrant tubular structures containing the capsi
10 ins, we deleted the gp64 gene from an AcMNPV bacmid and inserted F protein genes from three different
13 reverse genetics system, the "eight-in-one" bacmids (bcmd-RGFlu) showed higher efficiency of virus r
15 g counter-selection recombineering to modify bacmid bMON14272, a recombinant baculoviral genome, as w
16 s a rigorous characterization of recombinant bacmids but involves multiple steps, a limitation when m
18 In contrast, rescuing the vlf-1 knockout bacmid construct with a copy of VLF-1 that carries a mut
21 mphenicol acetyltransferase gene (cat) and a bacmid containing the AcMNPV genome in Escherichia coli.
23 n human cytomegalovirus (HCMV, strain AD169) bacmid-derived viruses to test their effects on virus re
25 re K-Rta is overexpressed, a K-bZIP knockout bacmid displayed an aberrant subcellular localization pa
28 dividual ESCRT-I or ESCRT-III gene and viral bacmid DNA or viral bacmid DNA that expressed DN forms o
29 ESCRT-III gene and viral bacmid DNA or viral bacmid DNA that expressed DN forms of ESCRT-I and ESCRT-
34 c toolbox is composed of a set of engineered bacmids expressing a fluorescent marker to monitor virus
36 CMV bacterial artificial chromosome plasmid (BACmid) expressing the nonshuttling UL84 mutant (NS84 BA
38 coexpression experiments, both single-mutant bacmids gave rise to infectious virus but the double mut
41 expression assays, studies of a lef-11-null BACmid indicate that LEF-11 is required for viral DNA re
44 rast, the substitution of RRV gN into a KSHV BACmid negatively affected the assembly of KSHV, indepen
45 ansfection of insect cells with lef-4 mutant bacmid, no viral progeny was produced, further defining
46 gN from KSHV, each of the KSHV-chimeric RRV BACmids restored virus replication and infectious spread
47 AN-KO/GUS, vAcAN-KO/GUS-Res, and a wild-type bacmid showed that the vAcAN-KO/GUS bacmid was able to r
48 0 to 1150 (BAC16Delta1100-1150, where BAC is bacmid) showed reduced replication and persistence of vi
49 similar to that detected for a gp64 knockout bacmid that served as a noninfectious control virus.
53 in cell culture, although a "repair" AcMNPV BACmid (vAc(lef11KO-REP)), which was generated by transp
54 d amplification of the genomes of the repair BACmid (vAc(lef11KO-REP)), wild-type AcMNPV, and a nonpr
55 nexpectedly, the resulting AcMNPV lef-6-null BACmid (vAc(lef6KO)) was able to propagate in cell cultu
58 thern analysis of Sf9 cells transfected with bacmid vAcAN-KO/GUS showed that transcription of late an
61 ocalization during infection using an AcMNPV bacmid virus that produces a functional AcMNPV FP25K-gre
62 ild-type bacmid showed that the vAcAN-KO/GUS bacmid was able to replicate to levels similar to those
63 n this report, we show that a vlf-1 knockout bacmid was able to synthesize viral DNA at levels simila
64 that the substitution of KSHVgM into the RRV BACmid was associated with attenuation in viral spread,
66 o investigate its function, an ac79-knockout bacmid was generated through homologous recombination in
67 2 during virus replication, an ac92-knockout bacmid was generated through homologous recombination in
68 nto the polyhedrin locus of the vAc(lef11KO) BACmid, was able to replicate in a manner similar to wil