ライフサイエンスコーパス: be purified by

1 ng its solubility in organic solvents, ImmE1 was purified by 1-butanol extraction of isolated membran

2 In most cases, the amide products can be purified by a simple filtration procedure using comme

3 The His-tagged enzyme is purified by a combination of Ni-NTA and orange A agar

4 The glycosphingolipid was purified by a combination of high-performance liquid

5 ) was formed even by 8 M 2-pic, if the 2-pic was purified by a novel Co(III)-affinity distillation pr

6 MHC was purified by a preparative continuous elution gel ele

7 This Mg2+-dependent endonuclease was purified by a series of chromatographic procedures.

8 Overexpressed SHI was purified by a single affinity chromatography step us

9 llulans NRRL Y-2311-1 produced on wheat bran was purified by a single-step chromatographic procedure.

10 mbinant soluble form in Escherichia coli and was purified by a two-step procedure to near-homogeneity

11 -dependent 1,2-PD degradation by S. enterica were purified by a combination of detergent extraction a

12 Spores were purified by a combination of isopycnic Percoll grad

13 ECoG signals were purified by a denoising procedure of wavelet decomp

14 copGFP(+) ICC from the jejunum were purified by a fluorescence-activated cell sorter an

15 Bound cytoplasmic proteins were purified by a photocleavable biotin tag and stretch

16 pounds, and many of these coupling compounds were purified by a simple precipitation-filtration techn

17 The resultant peptides were purified by a two-dimensional method: size exclusio

18 in-CBD fusion, and the stable fusion protein is purified by adsorption onto a chitin column.

19 via the target binding site, the complex can be purified by affinity capture via the peptide tag afte

20 more avidly to phosphoethanolamine and could be purified by affinity chromatography using phosphoetha

21 It has been purified by affinity chromatography and has a molec

22 ntigen recognized by the inhibitory antibody was purified by affinity chromatography and identified b

23 The latter form of the enzyme was purified by affinity chromatography and shown to hav

24 quently, protein produced in mammalian cells was purified by affinity chromatography and used to immu

25 The recombinant human Hsp72 was purified by affinity chromatography from insect cell

26 The protein was purified by affinity chromatography on an anti-SOD a

27 The expressed enzyme was purified by affinity chromatography on Ni(2+)-agaros

28 eEF-1 was purified by affinity chromatography on tRNA-Sepharos

29 The FCBP was purified by affinity chromatography using FC-linked

30 CPO was purified by affinity chromatography, and the purifie

31 P47 was purified by affinity chromatography, digested with e

32 e active fraction of the labeled preparation was purified by affinity chromatography.

33 A principal trophozoite cysteine protease was purified by affinity chromatography.

34 gestion with phage endolysin, InlA-MH(6)-Cws was purified by affinity chromatography.

35 Recombinant P69 produced in insect cells was purified by affinity chromatography.

36 cellulosome produced by the S mutant strain was purified by affinity digestion, characterized enzyma

37 Expressed enzymes were purified by affinity chromatography and analyzed by

38 g and cleavage-secretion, ACE-bound proteins were purified by affinity chromatography and characteriz

39 tic peptides from the photolabeled terminase were purified by affinity chromatography and reverse-pha

40 BDE) secreted by Yersinia pseudotuberculosis were purified by affinity chromatography and used as imm

41 immunoprecipitation with anti-SMN antibodies were purified by affinity chromatography from extracts o

42 Wild-type (wt) and all mutant CRPs were purified by affinity chromatography on PCh-, pneumo

43 Recombinant and native RPE65 were purified by affinity chromatography.

44 ing complexes into structural domains, which were purified by affinity chromatography.

45 scherichia coli and the recombinant proteins were purified by affinity chromatography.

46 a and lambda fractions of anti-6B antibodies were purified by affinity chromatography.

47 DEK autoantibodies and immune complexes were purified by affinity column chromatography and anal

48 Recombinant non-lipidated proteins were purified by affinity or ion exchange chromatography

49 DEK autoantibodies and ICs were purified by affinity-column chromatography and anal

50 he culture media of transfected HEK293 cells was purified by ammonium sulfate fractionation and nicke

51 tide hydroxamate-sensitive shedding activity was purified by ammonium sulfate precipitation, DEAE chr

52 ely 40 microM) and reduced when mitochondria are purified by an isoosmotic density-gradient method.

53 The stocks can be purified by an iodixanol (OptiPrep) gradient centrifu

54 Released O-glycans were purified by an optimized protocol to eliminate inte

55 th produce an antilisterial peptide that can be purified by anion-exchange and gel filtration chromat

56 While a portion of each polysaccharide can be purified by anion-exchange chromatography, the side g

57 ell, and testis extracts from which SBP2 has been purified by anion exchange and RNA affinity chromat

58 al transglutaminase of Physarum polycephalum was purified by anion exchange and hydrophobic chromatog

59 The protein was purified by anion- and cation-exchange chromatograph

60 The homohexamers were purified by anion-exchange and gel-filtration chrom

61 Peptides were purified by anion-exchange chromatography and seque

62 plex yields several peptide-DNA adducts that were purified by anion-exchange column chromatography.

63 eal brush border membrane protein (I100) has been purified by anionic glycocholate affinity chromatog

64 Cross-linked peptides were purified by avidin affinity chromatography and char

65 Mutant proteins were purified by avidin affinity chromatography, labeled

66 erial has an altered buoyant density and can be purified by banding in cesium chloride (CsCl) gradien

67 Additionally, the products may be purified by basic extraction or salt formation, avoid

68 to a vegetable field produces a protein that was purified by bioactivity-guided fractionation based o

69 ins interacting with the tr1 promoter region were purified by biotin-labeled DNA affinity chromatogra

70 The complex was purified by both standard biochemical techniques and

71 The glycosylinositol phospholipids were purified by butanol extraction of a Triton X-114-so

72 exchange with (18)F-fluoride, and the tracer was purified by C18 cartridge separation.

73 scherichia coli, and the recombinant protein was purified by CaM affinity chromatography.

74 , which we have named Pseudechetoxin (PsTx), was purified by cation exchange and RP-HPLC and has a mo

75 GUS), and 31 healthy volunteers (normal PCs) were purified by CD138(+) selection.

76 When infected and noninfected cells are purified by cell sorting, the former uniformly expre

77 lator cells, and the CD25(+)-activated cells were purified by cell sorting.

78 ted CD4(+) donor T cells that expressed CD25 were purified by cell sorting.

79 The 90Y-21T-BAD-Lym-1 products were purified by centrifuged molecular sieving column ch

80 lar lysate from this recombinant preparation was purified by cesium chloride density gradient centrif

81 The c subunit was purified by chloroform/methanol extraction and deter

82 Act d 12 and Act d 13 were purified by chromatographic procedures.

83 The heterodimer can be purified by chromatography on nickel-nitriloacetic ac

84 Recombinant E. coli MEP was purified by chromatography on DE-52 and phenyl-Sepha

85 The domains were purified by cloning.

86 f stationary phase, the iodoaziridines could be purified by column chromatography; the use of deactiv

87 Both proteins have been purified by column chromatography to >90% homogenei

88 lyacrylamide gel electrophoresis (SDS-PAGE), was purified by column chromatography and SDS-PAGE and s

89 BChE produced by coexpression with Q(N)PRAD was purified by column chromatography.

90 The nsLTP1 has been purified by combination of chromatographic techniqu

91 The byproduct was purified by combined ion exchange and reversed phase

92 TibA was purified by continuous-elution preparative sodium do

93 lectively cross-linked to the functional PLE was purified by conventional chromatography and identifi

94 ance of an ancillary vector in 293 cells and was purified by CsCl banding.

95 Ba1 was purified by CsCl gradient centrifugation and was fou

96 collagenase digestion and size fractionation were purified by CsCl density gradient centrifugation.

97 tite ELP-intein-target protein precursor can be purified by cycles of salt addition, heating and cent

98 The HbRC core was purified by DEAE ion-exchange chromatography and res

99 (Hs antigen) in the parent cell extract that was purified by DEAE Sephacel column chromatography and

100 stance is stable to mild base hydrolysis and was purified by DEAE-cellulose column chromatography.

101 Isolated cells were purified by density gradient.

102 Rat liver mitochondria were purified by differential and Percoll gradient centr

103 Exosomes from conditioned media were purified by differential centrifugation.

104 Islets were purified by discontinuous gradient centrifugation,

105 r from mouse beta TC-3 cell nuclear extracts was purified by DNA affinity chromatography and two-dime

106 The E2 binding protein was purified by DNA affinity chromatography.

107 The murine proteins were purified by DNA affinity isolation from the RAW264.

108 RLIP76 expressed in E. coli was purified by DNP-SG affinity chromatography.

109 Analysis of SCoV particles that were purified by either sucrose gradient equilibrium cen

110 Tia was purified by electroelution of outer membrane protein

111 The secreted proteins were purified by elution from columns of attached antibo

112 B and plasma cells from humans and baboons were purified by FACS sorting and characterized for anti

113 The cytolytic protein was purified by fast protein liquid chromatography (FPLC

114 Oms66 was purified by fast-performance liquid chromatography a

115 N'-acyl-N,N-dialkylformamidine intermediates are purified by flash-column chromatography and the puri

116 ipose tissue, greater than 10(4) eosinophils were purified by fluorescence-activated cell sorting (FA

117 Rat islets were dispersed and beta-cells were purified by fluorescence-activated cell sorting acc

118 Synovial bone and cartilage progenitors were purified by fluorescence-activated cell sorting and

119 Bone marrow-derived HSCs were purified by fluorescence-activated cell sorting and

120 rom the septal region of late embryonic mice were purified by fluorescence-activated cell sorting bas

121 DCs were purified by fluorescence-activated cell sorting or

122 gammadeltaT cells were purified by fluorescence-activated cell sorting.

123 terygoplichthys disjunctivus viscera trypsin was purified by fractionation with ammonium sulphate, ge

124 ether with tightly bound protein factors can be purified by gel filtration as a functional entity cal

125 entified keratan sulfate proteoglycan (KSPG) was purified by gel filtration (Superose 6; Amersham Pha

126 The active agent of this venom was purified by gel filtration and reverse-phase high pr

127 Ligand was purified by gel filtration, affinity precipitation o

128 Slow and fast migrating products were purified by gel electrophoresis and imaged by atomi

129 situ, and the telomere restriction fragments were purified by gel filtration chromatography.

130 ked, and the telomeric restriction fragments were purified by gel filtration.

131 PM proteins were purified by gel permeation, anion exchange, and NPA

132 parations of prothymosin alpha, one of which was purified by gentle means (the native protein, and a

133 The extracted polypeptide was purified by glutathione affinity column chromatograp

134 The fusion protein was purified by glutathione affinity, and CLIC-1 was rel

135 The overexpressed proteins were purified by glutathione-Sepharose 4B affinity chrom

136 Total GSTs were purified by GSH-affinity chromatography, and the is

137 00 mg/L of Escherichia coli culture) and can be purified by heat treatment; they can operate up to 75

138 ression levels, His-tagged Aquifex Amt could be purified by heating and nickel chelate affinity chrom

139 y solid-phase method using C18 cartridge and was purified by high performance liquid chromatographic

140 The radiolabeled peptide was purified by high-pressure liquid chromatography and

141 oproteinase Asp-N, and the peptide fragments were purified by high performance liquid chromatography.

142 hese classes with IC(50) values below 600 nm were purified by high pressure liquid chromatography, ch

143 Six peptides were purified by high-performance liquid chromatography

144 rom frozen tissue from two corneas with FSCA were purified by high-pressure liquid chromatography fol

145 t minimize artifacts, individual nucleosides are purified by HPLC and quantified by isotope-dilution,

146 ted with trypsin and the resulting fragments are purified by HPLC.

147 The 185 kDa protein was purified by HPLC, characterized by mass spectrometry

148 the photo-dimer-containing oligonucleotides were purified by HPLC (ion exchange and reverse phase) a

149 The cross-linked duplexes were purified by HPLC and characterized by enzymatic dig

150 S12) RNAs with short and long poly(A) tracts were purified by hybrid selection and analyzed by RT-PCR

151 The protease was purified by hydrophobic interaction chromatography o

152 ne retina mixed with radioactive carotenoids were purified by hydrophobic interaction, ion exchange,

153 After expression in E. coli, the scFv was purified by immobilized metal affinity chromatograph

154 The recombinant aspartyl proteinase was purified by immobilized metal affinity chromatograph

155 hydrogenase system of Rhodospirillum rubrum, was purified by immobilized metal affinity chromatograph

156 rypsin, and two ligand-cross-linked peptides were purified by immobilized aluminum affinity chromatog

157 late assay (Alexon, Inc., Sunnyvale, Calif.) was purified by immunoaffinity chromatography from super

158 Recombinant chsp10 was purified by immunoaffinity chromatography with M1.2.

159 The extracts containing the HAAs were purified by immunoaffinity chromatography and analy

160 ss-linking prior to detergent extraction and were purified by immunoaffinity chromatography.

161 Human maltase-glucoamylase was purified by immunoisolation and partially sequenced.

162 Labeled nascent transcripts are purified by immunoprecipitation, and transcript leve

163 ibonucleocapsid and forms a complex that can be purified by immunoprecipitation or ultracentrifugatio

164 Free CrSUMO96 was purified by immunoprecipitation and identified by ma

165 The reactive antigen was purified by immunoprecipitation and microsequenced;

166 . citri adhesion related protein P89 (SARP1) was purified by immunoprecipitation using anti-SARP1 mon

167 minant protein recognized by the immune sera was purified by ion exchange chromatography and reverse

168 ative UDP-l-Ara4N product generated in vitro was purified by ion exchange chromatography, and its str

169 dodecyl beta-D-maltoside, and the RC complex was purified by ion-exchange chromatography.

170 The PigA protein was purified by ion-exchange chromotography.

171 Ncd tail fusion proteins (TrxNT) were purified by ion exchange (S-Sepharose) and/or Talon

172 The aqueous extracts obtained were purified by ion exchange chromatography techniques

173 Subunits were purified by isoelectric focusing from extracts of c

174 Borrelial inner and outer membranes were purified by isopycnic centrifugation, and membrane

175 all of the compounds in our druglike library are purified by LC/MS/UV and are then controlled for ide

176 These complexes can be purified by lectin chromatography or by using Ni2(+)-

177 secreted env ectodomain (recombinant gp140) were purified by lentil-lectin chromatography and gel fi

178 The active compounds from 39 selected fungi were purified by liquid-liquid extraction and preparativ

179 olyhedrovirus of Culex nigripalpus (CuniNPV) were purified by Ludox density gradient ultracentrifugat

180 Murine marrow-derived stromal cells (MSCs) were purified by magnetic bead separation of cultured bo

181 th the MBP-SalA and MBP-SyrF fusion proteins were purified by maltose affinity chromatography.

182 gamma-glutamyltransferase [GGT]; EC 2.3.2.2) was purified by means of fast protein liquid chromatogra

183 The protease was purified by means of several chromatography steps.

184 nts of GTF-I from Streptococcus downei MFe28 were purified by means of a histidine tag.

185 The isotopes were purified by means of cation exchange chromatography

186 tosolic and mitochondrial SODs of C. sapidus were purified by means of ion-exchange, size-exclusion,

187 The resulting Fab/Rev complex was purified by metal ion affinity chromatography and ch

188 oximately 48-kDa His-tag derivative proteins were purified by metal affinity chromatography, and thei

189 The labeled proteins are purified by mini-scale affinity chromatography and a

190 e urine samples from the in vivo experiments were purified by mixed-mode cation-exchange solid-phase

191 m inclusion bodies, and the active component was purified by MMP-1 affinity chromatography.

192 permease containing a biotin acceptor domain was purified by monomeric avidin affinity chromatography

193 isease, retain fitness, and likely would not be purified by mosquito vectors.

194 The Sc2C2@Cs(hept)-C88 was purified by multistage high-performance liquid chrom

195 In this study, a new FMN hydrolase was purified by multistep chromatography after ammonium

196 Rubrerythrin was purified by multistep chromatography under anaerobic

197 The enzyme was purified by multistep chromatography.

198 ility to hydrolyze N-acetyl-L-methionine and was purified by multistep chromatography.

199 The enzyme was purified by multistep column chromatography.

200 Using human umbilical cord, native type XIX was purified by neutral salt extraction and by ion excha

201 o package high-titer viral vectors and could be purified by Ni-nitrilotriacetic acid affinity chromat

202 The recombinant His(6)-tagged TcNr was purified by Ni affinity chromatography.

203 The expressed proteins were purified by Ni-affinity column chromatography to yi

204 The resulting His6-PubC fusion proteins were purified by Ni-NTA affinity and gel filtration chro

205 HA), and the His-tagged recombinant proteins were purified by Ni-NTA chromatography.

206 the expressed peptide from Escherichia coli was purified by Ni2+ affinity chromatography, and rabbit

207 IPP isomerase from Thermus thermophilus HB27 was purified by Ni2+ affinity chromatography.

208 These derivatives were purified by Ni2+ affinity chromatography and examin

209 scherichia coli, and the recombinant protein was purified by nickel affinity chromatography and gel f

210 The IalB fusion protein was purified by nickel affinity chromatography and used

211 -terminal polyhistidine tag, and the protein was purified by nickel affinity chromatography.

212 When histidine-tagged PrgX (His-PrgX) was purified by nickel column chromatography from a stra

213 FqrB was purified by nickel interaction chromatography follow

214 f FliG, His-tagged FliM, FliN, FliH and FliI was purified by nickel-affinity chromatography.

215 scherichia coli, and the recombinant protein was purified by nickel-nitrilotriacetic acid affinity ch

216 Expressed VP5* and truncated VP5* proteins were purified by nickel affinity chromatography and assa

217 The His-tagged proteins were purified by nickel-nitrilo-triacetic acid chromatog

218 in Escherichia coli, and expressed proteins were purified by nickel-nitrilotriacetic acid (Ni-NTA) a

219 The His-tagged proteins were purified by nickel-nitrilotriacetic acid (Ni-NTA) c

220 il saponification, the unsaponifiable matter was purified by polymeric SPE and then the sterols and t

221 el of hybrids, only the final product has to be purified by precipitation or chromatography.

222 on vector, pPROEX-1, and recombinant protein was purified by preparative electrophoresis.

223 These compounds were purified by preparatory high performance liquid chr

224 Amplification System, and the chTNT-3 mutant was purified by protein A affinity and ion-exchange chro

225 Serum immunoglobulin was purified by protein A/G chromatography.

226 IgG was purified by protein-G chromatography, and xenoreacti

227 Moreover, all the products are purified by recrystallization from organic solvents.

228 of low bandgap indacenodithiophene polymers is purified by recycling SEC in order to isolate narrow

229 When necessary, these linear tetraphosphates are purified by reverse phase or anion exchange HPLC, yi

230 Crude CDS from bovine lung was purified by reverse-phase high-pressure liquid chrom

231 hemical cleavage, and the resultant peptides were purified by reverse phase high pressure liquid chro

232 he 5-guanidino-4-nitroimidazole modification were purified by reverse-phase and anion-exchange HPLC a

233 The hydroxamates were purified by reverse-phase and size-exclusion chroma

234 Two new theta-defensins, RTD-2 and RTD-3, were purified by reverse-phase high performance liquid c

235 deprotected, modified oligodeoxynucleotides were purified by reverse-phase HPLC and characterized by

236 The eluted peptides were purified by reverse-phase HPLC and tested for their

237 eaction of 3OHKYN and N(alpha)-acetyl lysine was purified by reversed phase high pressure liquid chro

238 ureus V8 protease, and a 3H-labeled fragment was purified by reversed-phase high-performance liquid c

239 The labeled product was purified by reversed-phase high-performance liquid c

240 (18)F-FB-IL2 was purified by reversed-phase high-performance liquid c

241 The new BBN constructs were purified by reversed phase high-performance liquid

242 tive and modified synthetic oligonucleotides were purified by reversed-phase HPLC using volatile ion-

243 The crosslinking activity was purified by RNA affinity chromatography and identifi

244 ntranslated region (3'-UTR) binding proteins were purified by RNA affinity chromatography from cytoso

245 These proteins were purified by RNA affinity column with biotinylated n

246 atic digestion, and peptides containing (3)H were purified by SDS-polyacrylamide gel electrophoresis

247 Most minimotifs have been purified by selection, with a 94% invariance, which

248 protein (OmpH) of Pasteurella multocida X-73 was purified by selective extraction with detergents, fo

249 nd the PLA2 activity in the soluble fraction was purified by Sephacryl S100 and DEAE Sephacel.

250 The interacting protein was purified by sequential DEAE and size exclusion chrom

251 a C-terminally histidine-tagged protein that was purified by sequential metal chelate affinity and ge

252 The smaller PP4 complex was purified by sequential phenyl-Sepharose, Source 15Q,

253 The TAT-Surv proteins were purified by sequential affinity and ion-exchange ch

254 The phosphonoglycans were purified by sequential organic solvent extractions,

255 DEK in supernatants and exosomes was purified by serial centrifugation and immunoprecipit

256 DEK in supernates and exosomes was purified by serial centrifugation and magnetic beads

257 usi spores derived from rhesus macaque feces were purified by serial salt-Percoll-sucrose-iodixanol c

258 osphate reagent to generate derivatives that are purified by silica gel chromatography and converted

259 Chiral allylboronates could be purified by silica gel chromatography and stored in t

260 th glutathione S-transferase (GST-Ad7PB) and was purified by single-step affinity chromatography.

261 Bovine hemolysate was purified by size exclusion chromatography, and one h

262 proteinase with a molecular mass of 28.7 kDa was purified by size-exclusion and cation exchange chrom

263 The construct was purified by size-exclusion chromatography and was fo

264 Ligands were purified by size exclusion and affinity chromatogra

265 he wild-type (alphaA-wt) and mutant proteins were purified by size exclusion chromatography and chara

266 Urinary LCs were purified by size exclusion chromatography using FPL

267 . coli, and the truncated alphaA-crystallins were purified by size-exclusion chromatography.

268 ated either with VSH-1 capsids or with tails were purified by sodium dodecyl sulfate-polyacrylamide g

269 The crosslinked RNA binding proteins (RBPs) are purified by solid-phase reversible immobilization (S

270 artridge Extraction (LLCE), the FFAs extract was purified by Solid Phase Extraction (SPE), methylated

271 lite of 8-iso-prostaglandin F(2alpha.) Urine was purified by solid-phase extraction and analyzed by U

272 (99m)Tc was purified by solid-phase extraction or biphasic excha

273 enylation of linoleic acid yielded LNO2 that was purified by solvent extraction, silicic acid chromat

274 e marrow hematopoietic stem cells (HSCs) can be purified by sorting Hoechst 33342-extruding side popu

275 idine-tagged actin are coexpressed, they can be purified by standard techniques and then separated us

276 compared to reactions using ligands that had been purified by standard methods.

277 The product was purified by stirring for 5 min with a 20% (w/v) susp

278 ated from the salivary gland of R. prolixus, was purified by strong cation exchange and reverse-phase

279 DESIGN AND ELVs released from adipose tissue were purified by sucrose gradient centrifugation and lab

280 Mutant mouse MDR3 P-glycoproteins were purified by the same procedure after growth of cell

281 The 22:6OOH was obtained from pure 22:6 and was purified by thin-layer and high-performance liquid c

282 an virus 40 transcription elongation complex was purified by this method and the topological linking

283 TGP1 was purified by three-column chromatographies, including

284 Both native and SeMet recombinant proteins were purified by three chromatographic steps, to yield p

285 The complex was purified by two different methods: conventional chro

286 A C-terminal 6xHis-tagged protein was purified by two-column chromatography.

287 sminogen after treatment of intact cells and was purified by two-dimensional gel electrophoresis and

288 ApoA-1 isoforms were purified by two-dimensional gel electrophoresis (2D

289 EVs have traditionally been purified by ultracentrifugation (UC), however UC ha

290 Lp(a) was purified by ultracentrifugation.

291 Nine proteins have been purified by using the system with yields ranging fr

292 FKBP52 was purified by using a prokaryotic expression plasmid c

293 fermentation and cell disruption, BoNT/A(Hc) was purified by using a three-step chromatographic proce

294 anti-CD3 and anti-CD28 beads, and Tr1 cells were purified by using an IL10 cytokine-capture assay an

295 The resulting oligosaccharides were purified by using Bio-Gel P4 gel permeation chromat

296 from rabbit hair, and IgE-reactive proteins were purified by using sequential chromatography.

297 Radiotracers were purified by using size-exclusion methods and evalua

298 lypeptides of molecular masses 25 and 65 kDa were purified by virtue of their ability to specifically

299 otides that contained 16-bp random sequences were purified by vnd/NK-2 affinity column chromatography

300 The fusion protein was purified by zinc-chelate chromatography, and the N-t