ライフサイエンスコーパス: bicistronic

1 coding region, indicating that the sgRNA7 is bicistronic.

2 , transcripts encoding RRV R15 and ORF74 are bicistronic.

3 er synthesized by coupled translation from a bicistronic (-1 frameshift) clone, or reconstituted from

4 on of the gI and gE genes formed an abundant bicistronic 3.5-kb mRNA, as well as an abundant 2.0-kb g

5 In this study we use bicistronic 4get IL-4 reporter mice to directly visualiz

6 d AAT-opt transgenes separately, or a single bicistronic AAV vector.

7 Using bicistronic ACT1-LUC constructs, elongating yeast riboso

8 We now report the use of a bicistronic adenoviral vector (Ad-CMV-D2R80a-IRES-HSV1-s

9 The IRES-based bicistronic adenoviral vector can potentially be used in

10 To address this issue, we have constructed a bicistronic adenoviral vector encoding the two proteins

11 We report here the construction of a single bicistronic adenoviral vector for tumor-specific Bax exp

12 mize potential toxicity of the TRAIL gene, a bicistronic adenoviral vector that expresses the green f

13 (F) protein, using plasmid cotransfection or bicistronic adenoviral vectors, the retargeted H protein

14 We constructed a bicistronic adenovirus bearing BDNF under cytomegaloviru

15 d-uPAR) or wild-type p16 cDNA (Ad-p16) and a bicistronic adenovirus construct in which both the uPAR

16 oth E1A and E1B genes under the control of a bicistronic AFP-E1A-IRES-E1B cassette yielded improvemen

17 The remaining four transcripts are bicistronic and encode a series of novel truncated isofo

18 n papillomavirus 16 (HPV16) E6E7 pre-mRNA is bicistronic and has an intron in the E6 coding region wi

19 genesis (5 or 7 amino acid residues) in both bicistronic and monocistronic HCV cell culture-based (HC

20 Using both bicistronic and monocistronic mRNAs containing the c- my

21 eening of KSHV 3'UTRs has identified several bicistronic and polycistronic transcripts as the novel t

22 extended 3'UTRs of the 5' proximal genes of bicistronic and polycistronic transcripts offer addition

23 transcripts and 3'UTRs has revealed abundant bicistronic and polycistronic transcripts.

24 otide resolution of RNA-Seq data revealed 15 bicistronic and tricistronic messages along the genome,

25 Moreover, we detected numerous bicistronic and tricistronic mRNA messages in the ascovi

26 s.c. injections of the bicistronic antisense construct into established tumors

27 Subcutaneous injections of bicistronic antisense constructs into established tumors

28 ls the existence of a functionally important bicistronic antiviral mRNA and suggests a widespread rol

29 y combining bidirectional promoter (BDP) and bicistronic approaches to drive coordinated expression o

30 hat Aep3p, previously shown to stabilize the bicistronic ATP8-ATP6 mRNA and facilitate initiation of

31 ote F(1) assembly, have normal levels of the bicistronic ATP8/ATP6 mRNAs but fail to synthesize Atp6p

32 Here, we developed a bicistronic biosensor encoding distinct repeat epitopes

33 A bicistronic BVDV (cp strain NADL) was created that expre

34 (2019) demonstrate that the bicistronic CACNA1A gene encodes a transcription factor

35 Of note, bicistronic cadBA is transcribed at low constitutive lev

36 einitiation occurs efficiently on subgenomic bicistronic calicivirus mRNAs, enabling synthesis of min

37 nstituted this process in vitro on two model bicistronic calicivirus mRNAs.

38 FP TRE to control an artificial E1A-IRES-E1B bicistronic cassette in an adenovirus 5 vector (Ad5) and

39 ce ganciclovir (GCV)-resistant colonies in a bicistronic cellular reporter system with HCV internal r

40 and demonstrate that for tumor suppression, bicistronic coexpression of p14ARF and p53 is superior t

41 The effect with bicistronic construct (Ad-uPAR-Cath B) was much higher t

42 The bicistronic construct (Ad-uPAR-uPA) infected glioblastom

43 This bicistronic construct (pUM) inhibited the formation of c

44 of UAS-regulated responder genes in a single bicistronic construct also appeared to accelerate silenc

45 Using a viral-based bicistronic construct and targeted proteomics to measure

46 e nucleoside analogue ganciclovir by using a bicistronic construct coexpressing the herpes simplex vi

47 roduced into HeLa cells, stably expressing a bicistronic construct encoding the hygromycin B phosphot

48 ted ex vivo with the Ad-uPAR-MMP-9 antisense bicistronic construct showed decreased invasiveness and

49 f SNB19 cells with the Ad-uPAR-uPA antisense bicistronic construct showed inhibited invasiveness and

50 nsfection with Ad-gp91ds-eGFP (an adenoviral bicistronic construct targeting NAD(P)H oxidase in fibro

51 (TVP1), were linked via IRES to generate the bicistronic construct TNHXS1-IRES-TVP1.

52 Here, we developed a bicistronic construct to drive expression of a CAR speci

53 n-tagged (HA-GlpF) polypeptides encoded by a bicistronic construct were expressed in bacteria.

54 /JFH (2a core), and H77/JFH (1a core), and a bicistronic construct, Bi-H77/JFH, all expressed both p8

55 plates that included the cSHMT 3'-UTR in the bicistronic construct.

56 ent protein by adenoviral gene transfer of a bicistronic construct.

57 potential problem further using promoterless bicistronic constructs coupled with RNase protection ass

58 By contrast, mice immunized with bicistronic constructs induced equivalent or higher leve

59 Use of bicistronic constructs revealed that TZD induction of IG

60 Interestingly, the presence of MV-F in the bicistronic constructs stimulated serum MV-specific immu

61 increased RHV replication of both mono- and bicistronic constructs, and CpG/UpA-dinucleotide optimiz

62 greater PRN titers than mice immunized with bicistronic constructs.

63 of the Y2441C substitution into the NADL or bicistronic cp viruses reconstituted the ncp phenotype.

64 We have generated a bicistronic Cre/LoxP reporter mouse line that pairs the

65 We used a bicistronic CXCR3 dual-reporter mouse, with each CXCR3 a

66 erfecta type II results from mutation of the bicistronic dentine sialophosphoprotein gene (DSPP ), we

67 findings, i.m. injection of the gp120/GM-CSF bicistronic DNA vaccine evoked a more extensive cellular

68 Thus, a bicistronic DNA vaccine that expresses gp120 and a trunc

69 A 100-fold lower dose of the gp120/GM-CSF bicistronic DNA vaccine was required to elicit detectabl

70 These results demonstrate that bicistronic DNA vaccines containing GM-CSF elicit remark

71 RNA molecule targeting the E7 region of the bicistronic E6 and E7 mRNA to induce RNA interference, t

72 y preventing translation initiation from the bicistronic eco29kIR-eco29kIM mRNA and causing its degra

73 -galactosidase (vector CT26), and the other (bicistronic) encodes eGFP and neomycin phosphotransferas

74 The S1 gene is bicistronic, encoding both the viral attachment protein

75 The S1 gene is bicistronic, encoding the viral attachment protein sigma

76 f a novel CXCR3 internal ribosome entry site bicistronic enhanced GFP reporter (CIBER) mouse in which

77 Finally, we show that bicistronic expression may be common to the voltage-gate

78 Human XIAP was delivered with bicistronic expression of green fluorescent protein (GFP

79 -subunit properties, we favored the use of a bicistronic expression plasmid encoding both GFP and a b

80 ternate mechanisms, we generated a series of bicistronic expression plasmids.

81 In addition, we use a bicistronic expression system that ensures recovery of s

82 Using bicistronic expression, we obtain an IRSp53 heterodimer

83 We developed an efficient lentiviral bicistronic fluorescent, ubiquitination-based cell cycle

84 The bicistronic genBA operon (formerly named celBA) of the o

85 Thus, this rare mammalian bicistronic gene coded for two tightly interacting brain

86 Here we have constructed a bicistronic gene containing both green fluorescent prote

87 iption of the major cold shock protein (CSP) bicistronic gene cspA1/A2 to increase by up to 300-fold.

88 labeled brain neurons in situ, indicative of bicistronic gene expression.

89 anopterin monophosphate (cPMP), requires the bicistronic gene molybdenum cofactor synthesis 1 (MOCS1)

90 cleotide repeat within exon 47 of CACNA1A, a bicistronic gene that encodes alpha1A, a P/Q-type calciu

91 Our combination of shRNA and TuD in a single bicistronic gene transfer vector derived from Adeno-asso

92 onstrate that the downstream cistron, in the bicistronic gene, is expressed to a much higher level wh

93 mRNA expression of CTRP5 and MFRP, which are bicistronic genes, was studied by semiquantitative RT-PC

94 was involved in morphogenesis by creating a bicistronic genome in which NS2-3 was restored in the se

95 osome entry site between the two proteins (a bicistronic genome).

96 greatly diminished in mice vaccinated with a bicistronic gp120-Tat DNA vaccine, compared with those i

97 ated Tat protein before vaccination with the bicistronic gp120-Tat DNA vaccine.

98 rein, we describe for the first time a novel bicistronic HC-Ad driving constitutive expression of her

99 efficacy with no overt toxicities using this bicistronic HC-Ad even in the presence of systemic Ad im

100 f the efficacy, safety, and toxicity of this bicistronic HC-Ad vector in an animal model of GBM stron

101 The bicistronic HC-Ad-TK/TetOn-Flt3L was delivered into intr

102 Using a bicistronic HCV replicon system expressing a luciferase

103 lacking paralog group 13 expression using a bicistronic HOXA13/EGFP retroviral vector.

104 izing human liver (HLM), rat liver (RLM) and bicistronic human-cyt P450 3A4 (bicis.-3A4) microsomes a

105 In the present study we used bicistronic IFN-gamma-enhanced yellow fluorescent protei

106 on, which overlaps with the promoter for the bicistronic K14/v-GPCR delayed early gene that is transc

107 at the K6a-specific siRNA strongly inhibited bicistronic K6a-luciferase gene expression in vivo.

108 However, the novel bicistronic K8/K8.1 mRNAs translated a little K8 and no

109 atency protein coexpressed with vFLIP from a bicistronic latency-specific mRNA, was found to prevent

110 Examination of the more abundant bicistronic latent RNAs revealed the presence of an effi

111 high-efficiency transduction of HUVECs with bicistronic lentiviral vector encoding Noggin and enhanc

112 r successful hiPSC induction, we generated 4 bicistronic lentiviral vectors encoding the 4 RFs co-exp

113 he Tax oncoprotein of HTLV on hematopoiesis, bicistronic lentiviral vectors were constructed encoding

114 Erp (OspE/F-related) proteins from mono-and bicistronic loci that are carried on up to 10 separate p

115 hat the organization of these two genes in a bicistronic locus has been selected during evolution to

116 dent VEGF mRNA translation, as assessed by a bicistronic luciferase reporter assay.

117 dependent mRNA translation, as assessed by a bicistronic luciferase reporter assay.

118 nternal ribosome entry site activity using a bicistronic luciferase reporter plasmid, but none was de

119 We confirmed this finding by generating bicistronic luciferase reporter plasmids, and we were ab

120 ndependent translation were assessed using a bicistronic luciferase reporter transfected into neurons

121 The retrovirus generates a bicistronic message encoding the gene of interest and GF

122 n elongation factor 1alpha promoter drives a bicistronic message encoding the genes for human NOSII a

123 Production of oncoprotein from a bicistronic message is accomplished in bitransgenic prog

124 eased the level of production of OrfA from a bicistronic message that also encodes Rev.

125 n GFP is expressed from an IRES element in a bicistronic message with Elk-1.

126 Expression of cwlD in the forespore is in a bicistronic message with the upstream gene ybaK.

127 tion, inhibition of ribosome scanning on the bicistronic message, through insertion of a synthetic st

128 cannot, such as short abortive transcripts, bicistronic messages and overlapping transcripts that di

129 The bicistronic microRNA (miRNA) locus miR-144/451 is highly

130 Mutational analysis of bicistronic minigenomes and recombinant EBOVs showed no

131 Transcription of overlapping genes in EBOV bicistronic minigenomes followed the stop-start mechanis

132 n proteins, MAVS and miniMAVS, from a single bicistronic mRNA and suggest that noncanonical translati

133 CACNA1A coordinates gene expression using a bicistronic mRNA bearing a cryptic internal ribosomal en

134 tiviral innate immunity, is expressed from a bicistronic mRNA encoding a second protein, miniMAVS.

135 hepatitis B virus (DHBV) pregenomic RNA is a bicistronic mRNA encoding the core and polymerase protei

136 A bicistronic mRNA expression system was used to demonstra

137 When this 5'-leader was placed in a bicistronic mRNA expression vector, it functioned as an

138 -mediated initiation were investigated using bicistronic mRNA expression vectors.

139 plate for reverse transcription and also the bicistronic mRNA for core and polymerase.

140 genome and the production of the full-length bicistronic mRNA from the 35S promoter.

141 ysis revealed that hlyBA were expressed as a bicistronic mRNA induced approximately 2.5-fold under lo

142 model in which translation of the ATP8/ATP6 bicistronic mRNA is coupled to the availability of F1 fo

143 pressed in PEL cell lines as a large spliced bicistronic mRNA of 3.2 kb that also encompasses the ups

144 litate translation of the second gene in the bicistronic mRNA system, this result suggested that the

145 IRES activity during in vitro translation of bicistronic mRNA templates, and in MCF-7 and HeLa cells

146 pped to the intercistronic region (ICR) of a bicistronic mRNA that we cloned from the MDV-transformed

147 rnal ribosome entry sequence (HCV IRES) in a bicistronic mRNA was increased relative to cap-dependent

148 Additionally, a low-abundance ycfX-cobB bicistronic mRNA was observed which could encode up to t

149 ynthetic pathway, are spe-cified by a single bicistronic mRNA with overlapping reading frames.

150 t translation from an internal position of a bicistronic mRNA, and unlike cap-driven translation, it

151 translated from the downstream position on a bicistronic mRNA, called the pregenomic RNA, through a p

152 0 produces the CinA and CinQ proteins from a bicistronic mRNA.

153 r, the speB-prsA operon was transcribed as a bicistronic mRNA.

154 lation of the second open reading frame in a bicistronic mRNA.

155 ading through the gene junction to produce a bicistronic mRNA.

156 16 (HPV-16) can be translated from a single, bicistronic mRNA.

157 y site located in the intergenic region of a bicistronic mRNA.

158 the mitochondrial genome and translated from bicistronic mRNAs containing both reading frames.

159 family of immediate-early genes, the mature bicistronic mRNAs have variable 5' leader sequences due

160 nant human adenoviruses were used to express bicistronic mRNAs in murine organs.

161 nant human adenoviruses were used to express bicistronic mRNAs in murine organs.

162 5' leader (designated 5L) from a variant of bicistronic mRNAs that encode the pp14 and RLORF9 protei

163 ith high sensitivity, direct transfection of bicistronic mRNAs, and insertion of an iron response ele

164 le and leads to production of two additional bicistronic mRNAs, K8/K8.1alpha and -beta.

165 and in MCF-7 and HeLa cells transfected with bicistronic mRNAs.

166 demonstrated by Northern blot analysis, and bicistronic N-U mRNA was also evident.

167 Confirmation of the bicistronic nature of CYP27 mRNA by epitope mapping of u

168 inserted into WASP- bone marrow cells with a bicistronic oncoretroviral vector also encoding green fl

169 l membrane protein that is translated from a bicistronic open reading frame encoded within subgenomic

170 ivergently transcribed from pigS-pmpABC is a bicistronic operon (blhA-orfY), which encodes a metallo-

171 families are encoded in widespread conserved bicistronic operon architectures that are reminiscent of

172 Gene bba24 is part of a bicistronic operon with bba25 that encodes the well-char

173 ospB is transcribed from a bicistronic operon with ospA, a known spirochete adhesio

174 s with homology to yadA and found three: the bicistronic operon yadBC (YPO1387 and YPO1388 of Y. pest

175 es encoding these proteins are arranged in a bicistronic operon, with the tbpB gene located upstream

176 cribed as monocistronic mRNA species or as a bicistronic operon.

177 We show that AbiE systems are encoded by bicistronic operons and function via a non-interacting (

178 RNA, thereby revealing the use of functional bicistronic operons in mammals.

179 stablished that relBE, relFG, and relJK form bicistronic operons that are cotranscribed and autoregul

180 Here, we create an optimized bicistronic optogenetic system using Arabidopsis thalian

181 nes that encode either MV-H or MV-F alone or bicistronic or fusion system vectors that encode both MV

182 ore splice genes and encodes many genes with bicistronic or polycistronic transcripts.

183 ORF56 was expressed at low abundance as a bicistronic ORF56/57 transcript that utilized the same i

184 The BDP system was then combined with a bicistronic organization of genes at both ends of the pr

185 Secretion was markedly lower with the bicistronic pHR-GM/CD vector (average, 225.7 pg/10(6) ce

186 ith the monocistronic pHR-CD80 vector or the bicistronic pHR-GM/CD vector became 75% to 95% CD80 posi

187 Bicistronic plasmid expression of the RPE65 Gly244Val mu

188 However, immunization with a bicistronic plasmid that coexpressed gp120 and GM-CSF un

189 A library of bicistronic plasmids encoding the enhanced blue and gree

190 Two bicistronic plasmids were constructed that encoded the p

191 viral oncoproteins, E6 and E7, from a single bicistronic pre-mRNA containing three exons and two intr

192 his report, we provide the evidence that the bicistronic pre-mRNA intron 1 contains three 5' splice s

193 express, respectively, a tricistronic and a bicistronic pre-mRNA, which undergo alternative splicing

194 viral oncogenes, are expressed from a single bicistronic pre-mRNA.

195 t partially overlaps the core (C) ORF on the bicistronic pregenomic RNA (pgRNA).

196 pment, directly activates transcription of a bicistronic primary transcript encoding miR-1-2 and 133a

197 ransient transfection assay performed with a bicistronic Renilla-HCV IRES-firefly luciferase reporter

198 destinies of telencephalic precursors using bicistronic replication defective retroviruses.

199 spreadsheets could be easily adapted to any bicistronic reporter assay system.

200 Bicistronic reporter assay systems have become a mainsta

201 he systematic analysis of data obtained from bicistronic reporter assay systems.

202 Using the bicistronic reporter assay, we examined whether morphine

203 PH2 5'-UTR was suggested by the conventional bicistronic reporter assay; however, further stringent e

204 evidence for cellular IRES activity rests on bicistronic reporter assays, the reliability of which ha

205 o-delivering a firefly luciferase/mutant K6a bicistronic reporter construct and mutant-specific siRNA

206 We find that in the context of a bicistronic reporter construct, HIV-1 gag IRES' activity

207 al tool used in these reports was the use of bicistronic reporter constructs in which the 5'-UTR was

208 By using bicistronic reporter constructs, we showed that the tran

209 the generation of a mouse model, in which a bicistronic reporter expressing a red fluorescent protei

210 roots, of transgenic seedlings carrying the bicistronic reporter gene construct.

211 This Foxp3 bicistronic reporter knockin mouse model should greatly

212 ed IL-17A enhanced green fluorescent protein bicistronic reporter mouse strains to analyze in situ pr

213 gation of the two IRESs residing in the same bicistronic reporter mRNA revealed functional synergism

214 and hsp70 also increased levels of a second bicistronic reporter of Httex1 expression, mKate2, and i

215 nction as an internal ribosome entry site in bicistronic reporter plasmids, and it requires the 5'-pr

216 rted expression of a downstream cistron in a bicistronic reporter system.

217 ndent Cd40 mRNA translation as assessed by a bicistronic reporter that contained the 5'-UTR of the Cd

218 ition of its cognate binding site within the bicistronic reporter transcript.

219 strons, RT-PCR to detect mRNA encoded by the bicistronic reporter with high sensitivity, direct trans

220 sertion of an iron response element into the bicistronic reporter.

221 rs and early erythroblasts with the use of a bicistronic retroviral system, and their effects on CFU-

222 om the SK-N-SH cell line and inserted into a bicistronic retroviral vector also encoding green fluore

223 The bicistronic retroviral vector construct allowed for coex

224 te its role in normal adult hematopoiesis, a bicistronic retroviral vector encoding GATA-2 and the gr

225 with a murine stem cell virus (MSCV)-based, bicistronic retroviral vector overexpressing PPCA and th

226 For this study we used a novel bicistronic retroviral vector that engineers expression

227 For this purpose, a bicistronic retroviral vector was engineered that effici

228 5a/b-deficient background was rescued with a bicistronic retrovirus encoding TEL/JAK2 and Stat5a.

229 at harbors the AHBA biosynthetic operon, the bicistronic RifA construct and the pccB and accA1 genes

230 ling activities, either coding region of the bicistronic RNA can rescue the deficiency phenotype.

231 CV IRES-mediated translation programmed by a bicistronic RNA in vivo.

232 In the presence of Rev, this exon of the bicistronic RNA is skipped in a fraction of the spliced

233 Bicistronic RNA reporter transfection assays did not val

234 es from human papillomavirus 16 (HPV16) E6E7 bicistronic RNA was found to be a potent siRNA that supp

235 Importantly, the E6E7 bicistronic RNA with a mutant BPS and inefficient splici

236 ines with separate lentiviruses expressing a bicistronic RNA with a selectable marker as the first op

237 t vGPCR-containing transcripts are K14/vGPCR bicistronic RNAs that are strongly induced during lytic

238 secretion it can be incorporated in a novel bicistronic secretion system.

239 We describe bicistronic single-exon Tat (72-amino-acid Tat [Tat72])-

240 am open reading frame (ORF) of the imprinted bicistronic Snurf-Snrpn locus in the mouse.

241 protein halves, termed Split-ORFs, from the bicistronic SRSF7-PCE transcript.

242 beta-galactosidase or tauGFP, by virtue of a bicistronic strategy that leaves the coding region of th

243 he effect of ROS on HCV replication, using a bicistronic subgenomic RNA replicon and a genomic RNA th

244 report the construction and application of a bicistronic system, involving the internal ribosome entr

245 By constructing a bicistronic system, our data support that the mRNA encod

246 NA and regulates alternative splicing of the bicistronic tat/rev mRNA.

247 etion of CUGBP1 decreased IRES activity from bicistronic templates that included the cSHMT 3'-UTR in

248 OxyR-dependent tpx promoter resulting in the bicistronic tpx/rbp mRNA.

249 Northern analysis reveals expression of the bicistronic transcript and in vivo imaging of GFP and lu

250 t between two open-reading frames of a plant bicistronic transcript can mediate translation of the se

251 e trxB mRNA was cotranscribed with lolA as a bicistronic transcript or was present as a monocistronic

252 meric gene (named BIO3-BIO1) also produces a bicistronic transcript potentially encoding separate mon

253 through the gene-end sequence to generate a bicistronic transcript was enhanced compared to the obse

254 log of the p8 subunit (Dmp8) is encoded in a bicistronic transcript with the homolog of the Swc6/p18(

255 s ORF59 was expressed in high abundance as a bicistronic transcript, with a short 5' UTR and a long 3

256 rotein and mtTFB1 are encoded on a bona fide bicistronic transcript.

257 In eukaryotic cells, bicistronic transcripts are not common, and in some case

258 ese myogenic microRNAs, which are encoded by bicistronic transcripts or are nestled within introns of

259 previously undescribed viral RNAs, including bicistronic transcripts predicted to encode E5 and L2 or

260 Moreover, ~40% of bicistronic transcripts showed negative correlation in t

261 on initiation has been exploited to generate bicistronic transcripts that function in animal cells.

262 Type I splicing results in bicistronic transcripts with two open reading frames, of

263 Using transgenic mice containing VEGF-LacZ bicistronic transcripts, we found that inhibition of glo

264 e, tTA(off) activates the transcription of a bicistronic transgene encoding PDX1 and an enhanced gree

265 ly repress translation in mammalian cells, a bicistronic translational repression assay was developed

266 first two methionine codons in ORF3 and was bicistronic; two closely spaced methionine codons in dif

267 uestion by examining the expression of model bicistronic (v-cyclin/LUC) transcripts in which a lucife

268 mRNA was inserted between the two genes of a bicistronic vector and transfected into various cell lin

269 DR) type 1, is linked to ANP expression on a bicistronic vector and was coexpressed in the human kera

270 after subcloning SPGP cDNA into a retroviral bicistronic vector capable of expressing both SPGP and t

271 We transfected CHO-K1 cells with a bicistronic vector co-expressing GFP and arginase and se

272 Expression of bicistronic vector constructs showed that the 5'-UTRs of

273 n high yields in Escherichia coli by using a bicistronic vector encoding the PTP1B tyrosine phosphata

274 cell at a fixed relative level via a single bicistronic vector led to robust, uniformly normal angio

275 These data represent the first report of a bicistronic vector platform driving the expression of tw

276 In fact, T cells engineered with a bicistronic vector that concurrently expresses catalase,

277 idues Asp-90 and Arg-269 were generated in a bicistronic vector that encodes a 6-histidine-tagged ham

278 and expression of P450 and P450R on a single bicistronic vector using an internal ribosomal entry sit

279 (with NADPH-P450 reductase expressed using a bicistronic vector) were used to determine kcat and Km v

280 Using a bicistronic vector, we expressed EpoRm together with an

281 -driven messenger RNA were detected with the bicistronic vector, which may account for its poor expre

282 boxylase and the VKORC1 cDNA constructs in a bicistronic vector.

283 AUG 184 permits internal ribosome entry in a bicistronic vector.

284 ession of marker genes was observed with the bicistronic vector.

285 GM-CSF) and CD80 in separate vectors or in a bicistronic vector.

286 roach by adenoviral-mediated delivery of the bicistronic vector.

287 The use of bicistronic vectors carrying internal ribosome entry sit

288 These findings support the use of SIRES bicistronic vectors for a better assessment of therapeut

289 Using these sequences, we prepared two bicistronic vectors for mammalian expression of a repres

290 dimers can be produced by co-expression from bicistronic vectors in bacteria and that the co-expressi

291 Use of bicistronic vectors permitted us to assess more carefull

292 ern blotting, pulse labeling, and the use of bicistronic vectors showed increased cap-dependent trans

293 Bicistronic vectors were used to introduce compound hete

294 Single-gene vectors, bicistronic vectors, and multigene vectors expressing up

295 CRV expressing a single reporter gene from a bicistronic viral mRNA (rTCRV/GFP).

296 The bicistronic viral RNA, which is transcribed at the late

297 recently reported on intragenomic replicons, bicistronic viral transcripts expressing an authentic re

298 This bicistronic virus exhibited slightly slower growth kinet

299 A splice variant is bicistronic, with distinct but overlapping reading frame

300 mal deficiency in zebrafish that removes the bicistronic wnt8 locus.