ライフサイエンスコーパス: bilin

1 covalent attachment of linear tetrapyrroles (bilins).

2 ctor heme, and the light-harvesting molecule bilin.

3 higher-energy bands were from more isolated bilins.

4 coupling for the second excited state of the bilins.

5 ate exciton splitting between closely-spaced bilins.

6 hytochrome without the addition of exogenous bilins.

7 get of phyllobilins and as a novel target of bilins.

8 he signal of the carotenoid from that of the bilins.

9 min B(12)), coenzyme F(430), heme d (1), and bilins.

10 omains and has 7 potential binding sites for bilins.

11 excited state of one of the allophycocyanin bilins.

12 raethyl-7,13-dimethyl-10-thia-(21H,2 3H,24H)-bilin-1,19-dione (1), was synthesized from 8-(2-carboxye

13 eption: reversible photoisomerization of the bilin 15,16 double bond.

14 gion upstream of the PAS domain knot and the bilin A and B pyrrole rings.

15 m studies with purified proteins showed that bilin absence reduces the strength of alphabeta interact

16 ecombinant CpcT was used to perform in vitro bilin addition assays with apophycocyanin (CpcA/CpcB) an

17 No significant bilin addition took place in a similarly engineered E. c

18 No significant bilin addition took place in a similarly engineered E. c

19 far-red reversible and a second class whose bilin adducts are nonphotochromic.

20 classes of bilin lyase domains--those whose bilin adducts are red, far-red reversible and a second c

21 ole-exome sequencing of coexisting low-grade BilIN (adenoma), high-grade BilIN, and carcinoma lesions

22 Bilin amides were also assembled with BV-type and phytob

23 gio viridissima ssp. nov. from the Sittaung, Bilin and Bago rivers, I. pugio daweiensis ssp. nov. fro

24 netic and evolutionary relationships between BilIN and carcinoma remain unclear.

25 d crystallographic models, revealed that the bilin and GAF domain dynamically transition via breakage

26 isting low-grade BilIN (adenoma), high-grade BilIN, and carcinoma lesions, and normal tissues from th

27 ds of phycocyanin 612 originated from paired bilins, and the two higher-energy bands were from more i

28 ygenic phototrophs, chlorophylls, hemes, and bilins are synthesized by a common branched pathway.

29 Using the bilins as naturally occurring reporter groups, phycocyan

30 gene network implicates a widespread use of bilins as retrograde signals in oxygenic photosynthetic

31 bunits, covalent modification of subunits by bilin attachment and formation of the primary assembly u

32 chromophore and the apoprotein that promote bilin attachment and photointerconversion between the sp

33 Rs) are photoswitchable linear tetrapyrrole (bilin)-based light sensors in the phytochrome superfamil

34 iscuss recent progress in the development of bilin-based fluorescent proteins.

35 bsorption triggers photoisomerization of the bilin between the 15Z and 15E photostates.

36 the recombinant apoproteins were tested for bilin binding and phytochrome photoactivity.

37 structure of a far-red PBS core, showing how bilin binding in the alpha-subunits of allophycocyanin p

38 t encodes a large protein with two potential bilin binding sites, were amplified, and the recombinant

39 romes, CBCRs require only the GAF domain for bilin binding, chromophore ligation, and full, reversibl

40 identified a glutamate residue critical for bilin binding.

41 omes (CBCRs) are small, linear tetrapyrrole (bilin)-binding photoreceptors in the phytochrome superfa

42 es were generated by changing the codons for bilin-binding cysteines to alanine residues.

43 three-dimensional solution structure of the bilin-binding domain as Pfr, using the cyanobacterial ph

44 he roles of conserved amino acids within the bilin-binding domain of Deinococcus radiodurans bacterio

45 d cysteine at different positions within the bilin-binding GAF domain (cGMP-specific phosphodiesteras

46 acteriochrome (CBCR) proteins that share the bilin-binding GAF domain of phytochromes but sense other

47 nserved tyrosine residue (Tyr176) within the bilin-binding GAF domain of the cyanobacterial phytochro

48 hyl-accepting chemotaxis proteins containing bilin-binding GAF domains capable of directly sensing li

49 Both clusters encode bilin-binding globin proteins, phycobiliprotein paralogs

50 lengths of light and many possess a range of bilin-binding photoreceptors belonging to the phytochrom

51 This review adds the bilin-binding phytochromes to the Chemical Reviews thema

52 A 1.16-A resolution crystal structure of the bilin-binding pocket in the dark-adapted red light-absor

53 of phyB mutants was generated affecting the bilin-binding pocket that altered photochemistry, therma

54 key amino acids that form a solvent-shielded bilin-binding pocket, and reveals an unusually formed de

55 parental lipocalin, the naturally occurring bilin-binding protein (BBP).

56 ral and spectroscopic comparisons with other bilin-binding proteins together with site-directed mutag

57 ide unambiguous evidence that the N-terminal bilin-binding region of BphP also provides a dimerizatio

58 ond GAF domain, the tongue region, seals the bilin-binding site in the GAF1 domain from solvent acces

59 s of allophycocyanin paralogs can modify the bilin-binding site to red shift the absorbance spectrum.

60 A system reconstituting bilin biosynthesis in Escherichia coli was modified to u

61 itution of bilin biosynthesis to investigate bilin biosynthesis in streptophyte algae.

62 analysis and heterologous reconstitution of bilin biosynthesis to investigate bilin biosynthesis in

63 protein paralogs that we designate as BBAGs (bilin biosynthesis-associated globins).

64 pectroscopy supports the conclusion that its bilin chromophore adopts a similar conformation to the r

65 an N-terminal domain that covalently binds a bilin chromophore and a C-terminal region that transmits

66 isms depends on key interactions between the bilin chromophore and the apoprotein that promote bilin

67 numerous reversible interactions between the bilin chromophore and the associated polypeptide.

68 that underpin photochemistry of the coupled bilin chromophore and the ensuing conformational changes

69 nvolves intricate interactions between their bilin chromophore and the protein environment.

70 at involves a basic E/Z isomerization of the bilin chromophore and, in certain cases, the breakage of

71 CRs utilize a basic E/Z isomerization of the bilin chromophore as the primary step in their photocycl

72 reveal diurnal regulation of phytochrome and bilin chromophore biosynthetic genes in Micromonas.

73 F), and Phy-specific (PHY) domains, with the bilin chromophore covalently-bound within the GAF domain

74 Phytochromes are photoreceptors with a bilin chromophore in which light triggers the conversion

75 Zinc blot analyses confirm that a bilin chromophore is covalently bound to the algal phyto

76 erved GAF domain Tyr residue, with which the bilin chromophore is intimately associated, performs a c

77 t in the red light-absorbing (Pr) state, the bilin chromophore of the Deinococcus radiodurans proteob

78 are widely distributed photoreceptors with a bilin chromophore that undergo a typical reversible phot

79 loits reversible light-driven changes in the bilin chromophore to initiate a variety of signaling cas

80 pling photoreversible isomerization of their bilin chromophore to various signaling cascades.

81 HO family are important for synthesizing the bilin chromophore used to assemble photochemically activ

82 pport photochemical isomerization of a bound bilin chromophore, a process that triggers a conformatio

83 rminal region that covalently binds a single bilin chromophore, followed by a carboxy-terminal dimeri

84 idic residue to stabilize protonation of the bilin chromophore.

85 d Pfr, distinguished by Z/E isomers of their bilin chromophore.

86 eceptors that utilize a linear tetrapyrrole (bilin) chromophore covalently bound within a knotted PAS

87 n of a covalently-bound linear tetrapyrrole (bilin) chromophore located in a conserved photosensory c

88 development, using the linear tetrapyrrole (bilin) chromophore phytochromobilin (PPhiB).

89 s a covalently attached linear tetrapyrrole (bilin) chromophore to sense light.

90 hycocyanobilin (PCB), a linear tetrapyrrole (bilin) chromophore, as the light-harvesting pigment that

91 Bilin chromophores and bilirubin are involved in relevan

92 CikA covalently bound bilin chromophores in vitro, even though it lacks the ex

93 0 and 2 g/L protein concentration with eight bilin chromophores.

94 he biliproteins, which have covalently bound bilin chromophores.

95 e photoisomerization of linear tetrapyrrole (bilin) chromophores to measure the ratio of red to far-r

96 s using cysteine-linked linear tetrapyrrole (bilin) chromophores to regulate biological responses to

97 initiated by light-driven isomerization of a bilin cofactor, which triggers protein structural change

98 anonical Phys by having a Pr ZZZsyn,syn,anti bilin configuration but shifted to the activated positio

99 How changes in bilin conformation affect output by these photoreceptors

100 e members of the phytochrome (phy) family of bilin-containing photoreceptors are major regulators of

101 ontrolled by the phytochrome (Phy) family of bilin-containing photoreceptors that detect red and far-

102 mes (Phys) encompass a diverse collection of bilin-containing photoreceptors that help plants and mic

103 Phytochromes are a collection of bilin-containing photoreceptors that regulate a diverse

104 Phytochromes are a collection of bilin-containing photoreceptors that regulate numerous p

105 mediated by the phytochrome (Phy) family of bilin-containing photoreceptors that reversibly intercon

106 easured by the light-induced rotation of the bilin D-pyrrole ring that triggers conformational change

107 We propose that different directions of bilin D-ring rotation account for these distinct classes

108 ults suggest that phycocyanin instability in bilin-deletion mutants is a consequence of diversion of

109 Our discovery of a bilin-dependent nuclear gene network implicates a widesp

110 d tetrapyrrole metabolism in plastids due to bilin depletion.

111 lterations whereby photoisomerization of the bilin drives nanometer-scale movements within the Phy di

112 pes of PBs takes place on an allophycocyanin bilin emitting at 660 nm (APC(Q)(660)) with a molecular

113 scuss how these are correlated, from how the bilin environment affects the chromophore to how light i

114 phycoerythrin 545 were suggested to have one bilin in each monomeric (alphabeta) unit of the dimer (a

115 of the A and D pyrrole rings, sliding of the bilin in the GAF pocket, and the appearance of an extend

116 These include residues that fix the bilin in the pocket, coordinate the pyrrole water, and p

117 ecies that lack phytochromes, can synthesize bilins in both plastid and cytosol compartments.

118 ed BVR lines implicate a regulatory role for bilins in plastid development or, alternatively, reflect

119 The potential role of bilins in subunit structure and assembly is examined in

120 owever, the charge-transfer character of the bilins in the allophycocyanin-containing segments locali

121 a cancerous niche, leading to the subsequent BilIN-independent path.

122 nlike wild-type PcyA, both mutants possess a bilin-interacting axial water molecule that is ejected f

123 methods combined with NMR data show that the bilin is fully protonated in the Pb and Pg states and th

124 Biliary tract intraepithelial neoplasia (BilIN) is the common benign tumor that is suspected to b

125 inct red-shift mechanisms involving cationic bilin lactim tautomers stabilized by a constrained all-Z

126 lving delocalized optical excitations of the bilin (linear tetrapyrrole) chromophores in intact phyco

127 When CpcSU was assayed for bilin lyase activity in vitro with phycocyanobilin (PCB)

128 for cyanobacterial phytochrome 2, possessed bilin lyase activity, revealing two distinct classes of

129 We also show that phyB's N-terminal bilin lyase domain (BLD) and PHY domain interact directl

130 a 130-180 amino acid motif that delimits the bilin lyase domain, a subdomain of the extended phytochr

131 d by pattern searches represents a bona fide bilin lyase domain.

132 of highly conserved charged residues within bilin lyase domains of nearly all members of the extende

133 activity, revealing two distinct classes of bilin lyase domains--those whose bilin adducts are red,

134 The CpeS bilin lyase ligated PEB at both Cys(82) and Cys(139) of

135 We conclude that CpeF is the bilin lyase responsible for attachment of the doubly lig

136 Therefore, CpcSU is the bilin lyase-responsible for attachment of PCB to Cys-82

137 Specific bilin lyases are hypothesized to catalyze each PEB ligat

138 fied and characterized so far, and the other bilin lyases are unknown.

139 -59 of CpeB and together with other specific bilin lyases contributes to the post-translational modif

140 phores are ligated to specific cysteines via bilin lyases, and some of these enzymes, called lyase is

141 ly attached to the protein chain by specific bilin lyases.

142 lated from the others, and the remaining six bilins may be in pairs.

143 the hydrogen out-of-plane vibrations of the bilins mediate non-adiabatic relaxation of a manifold of

144 plore the role of the propionate moieties in bilin metabolism, we report the semisynthesis of mono- a

145 substitution experiments using semisynthetic bilin monoamides, which indicate that the propionate sid

146 Monomers were stable and had bilin optical spectra different from the alpha2beta2 dim

147 The bilin organization of three cryptomonad biliproteins (ph

148 tron transfers from ferredoxin to protonated bilin:PcyA complexes.

149 Linear tetrapyrroles (bilins) perform important antioxidant and light-harvesti

150 her, our data support a toggle model whereby bilin photoisomerization alters GAF/PHY domain interacti

151 Pr/Pfr comparisons show that bilin phototransformation alters PSM architecture culmin

152 s, SyA-Cph1 and SyB-Cph1 covalently bind the bilin phycocyanobilin via their cGMP phosphodiesterase/a

153 Whereas incorporation of the native bilin phytochromobilin into PhyB confers robust Pfr -->

154 Phytochromes bind a bilin pigment that switches its isomeric state upon abso

155 astoris were able to convert biliverdin to a bilin pigment, which produced a native difference spectr

156 ding from the Phy-specific domain toward the bilin pocket.

157 on of the porphyrin-like conformation of the bilin precursor to a more extended conformation.

158 s subfamilies have been shown to incorporate bilin precursors with larger pi-conjugated chromophores,

159 . radiodurans phytochrome, we show that this bilin preference was partially driven by the change in b

160 ion at the C(15)=C(16) methine bridge of the bilin prosthetic group.

161 The bilin prosthetic groups of the phytochrome photoreceptor

162 the biosynthesis of the linear tetrapyrrole (bilin) prosthetic groups of cyanobacterial phytochromes

163 ns with cysteine-linked linear tetrapyrrole (bilin) prosthetic groups.

164 anaerobic assay protocol, optically detected bilin-protein intermediates, produced during the PcyA ca

165 imensional structural results better clarify bilin/protein interactions and help explain how higher p

166 g state illuminated the intricate network of bilin/protein/water interactions and confirmed the proto

167 We previously showed that bound bilin radical intermediates could be detected by low tem

168 This interconversion occurs via semireduced bilin radical intermediates that are profoundly stabiliz

169 of a remarkable combination of farnesylated bilins (recently identified, tentative heme A catabolite

170 n IXalpha (BV), and the ferredoxin-dependent bilin reductase (FDBR) PcyA then converts BV into PCB.

171 de chains in substrate discrimination by two bilin reductase families while further underscoring the

172 tase is a member of the ferredoxin-dependent bilin reductase family and catalyzes two vinyl reduction

173 e to this conversion: a heme oxygenase and a bilin reductase with discrete double-bond specificity.

174 d of the representative ferredoxin-dependent bilin reductase, phycocyanobilin:ferredoxin oxidoreducta

175 operon consisting of a heme oxygenase and a bilin reductase, these studies establish the feasibility

176 ubsequent action of two ferredoxin-dependent bilin reductases (FDBRs).

177 res requires members of ferredoxin-dependent bilin reductases (FDBRs).

178 ynthesized by different ferredoxin-dependent bilin reductases (FDBRs): PPhiB is synthesized by HY2, w

179 chlorophyll catabolite reductases, which are bilin reductases involved in chlorophyll catabolism in p

180 or phytochromobilin, genes encoding putative bilin reductases were identified in the genomes of vario

181 reduced subsequently by ferredoxin-dependent bilin reductases with different double-bond specificitie

182 investigations defined three new classes of bilin reductases with distinct substrate/product specifi

183 Their catabolites, the phyllobilins and the bilins, respectively, share not only structural features

184 scale movements within the Phy dimer through bilin sliding, hairpin reconfiguration, and spine deform

185 We also discovered that the nature of the bilin strongly influences Pfr stability.

186 g substituents alters the positioning of the bilin substrate within the enzyme, profoundly influencin

187 P Phi B synthase has a high affinity for its bilin substrate, with a sub-micromolar K(m) for BV.

188 ctron-coupled proton transfers to protonated bilin substrates buried within the phycocyanobilin:ferre

189 involved greater fitness using more reduced bilins, such as phycocyanobilin, combined with a switch

190 utative binding domains for chlorophylls and bilins, suggesting these proteins may function as a rese

191 Phytochromes are photoreceptors using a bilin tetrapyrrole as chromophore, which switch in canon

192 e light-driven conformational changes in the bilin to altered contacts between the adjacent output do

193 Propagation of the light signal from the bilin to the output module likely depends on the dimeriz

194 network of embedded pigment molecules called bilins to the photosynthetic reaction centres.

195 es for efficient energy migration, and other bilins transfer energy to this pair, extending the wavel

196 Our studies also suggest that bilins trigger critical metabolic pathways to detoxify m

197 Photoisomerization of the bilin triggers photoconversion of the CBCR input, thereb

198 heme and heme-derived linear tetrapyrroles (bilins), two critical metabolites respectively required

199 rge hydrophobic cavity that accommodates two bilins, two luteins, and four phosphatidylcholines, all

200 Phyllobilins, tetrapyrrolic, bilin-type chlorophyll degradation products, are abundan

201 ophore that can potentially be produced upon bilin uptake by any living cell expressing an apophytoch

202 The resulting bilin was incorporated into model cyanobacterial photore

203 nin 612, a major surprise was that a pair of bilins was apparently not found across the monomer-monom

204 monomer-monomer interface, but the remaining bilins were distributed as in the other two cryptomonad

205 Attachment of the central bilin, which is common to all biliprotein subunits, may

206 iven rotation within the covalently attached bilin, which then triggers a series of protein conformat

207 A hypothesis is that the coupled pair of bilins within the monomeric units offers important advan

208 The paired bilins within the protein monomers contained the lowest-