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2 treptavidin-coated gold nanoparticles to the biotinylated Ab2 in order to enhance the sensor response
13 in of CEACAM1-SF and was covalently bound to biotinylated and T457C-modified peptide in the presence
18 magnetic microbeads (NA-MBs) modified with a biotinylated-anti-dsDNA (b-dsDNA) as efficient magnetic
20 of a competitive immunoassay using secondary biotinylated antibodies and streptavidin-conjugated Au n
21 hose surface is functionalized with suitable biotinylated antibodies and then coated with silver nano
22 e first functionalized with streptavidin and biotinylated antibodies and then used to immobilize and
23 titive immunoassays on the assay zones using biotinylated antibodies labeled with streptavidin-conjug
24 array, functionalized with streptavidin and biotinylated antibodies specific for Escherichia coli K-
25 a epoxide-azide coupling chemistry, to which biotinylated antibodies targeting cell surface receptors
28 the pCBAA coating, (II) binding of secondary biotinylated antibody (Ab2) to previously captured bacte
30 and a two-step detection scheme involving a biotinylated antibody that efficiently reduces nonspecif
32 be was integrated with sandwich immunoassay, biotinylated antibody, and avidin-conjugated GOx for the
34 tavidin magnetic beads coated with a generic biotinylated antihuman capture reagent, (2) "on-bead" di
35 ility of the bacterial biotin ligase BirA to biotinylate any protein that carries a short peptide (Av
37 re used as reporter particle scaffolds, with biotinylated aptamers attached via avidin-biotin linkage
40 interference was found in 9 of the 23 (39%) biotinylated assays compared with none of the 14 nonbiot
43 When desired, however, streptavidin plus the biotinylated bait can be completely removed by 3min inje
44 method for the switchable immobilization of biotinylated bait molecules on a new desthiobiotin surfa
47 eneration sequencing libraries commonly uses biotinylated baits to capture the desired sequences.
54 glass slides, followed by immobilization of biotinylated capture antibodies through a streptavidin b
55 oated on a PCR plate to capture complexes of biotinylated capture antibody, antigen, and DNA-labeled
57 etected in a double-tagging approach using a biotinylated capture probe for immobilization on magneti
63 ed at several time points, and tagged RNA is biotinylated, captured on streptavidin beads and sequenc
64 h node homing of leukocytes), the ability of biotinylated CCL19 with magnetic bead sorting to enrich
66 ble, in an immune-based assay, by coupling a biotinylated chemoprobe to a warhead capable of selectiv
67 nd, second, to control the immobilization of biotinylated cholera toxin antigen on copper coordinated
68 shots of the ciliary proteome by selectively biotinylating ciliary proteins using a cilia-targeted pr
69 s the advantages of the presence of a single biotinylated cofactor precisely localized within the bio
70 idin (Sav) and the noncooperative binding of biotinylated cofactors impose two limitations on the gen
71 Sav, and (ii) the noncooperative binding of biotinylated cofactors to Sav may lead to an erosion in
75 le in structure, and more effective than its biotinylated counterpart in promoting glutamylation acti
76 ibonucleoside crosslinking and an innovative biotinylated Cp retrieval method, here we comprehensivel
77 ne reductase results upon incorporation of a biotinylated Cp*Ir moiety (Cp* = C5Me5(-)) within homote
79 biotinylated knottin-RGD peptide instead of biotinylated cyclo[RGDfK] (as reported by Piras et al.),
80 lphavbeta5 and alpha5beta1, while binding of biotinylated cyclo[RGDfK] was very weak and only detecta
83 e directly labeled on surface lysines with a biotinylated derivative of the small ubiquitin homologue
84 tial delivery of 100 muL of bacteria sample, biotinylated detection antibodies, fluorescent streptavi
85 r step and delivers (i) the sample, (ii) the biotinylated detection antibody, (iii) streptavidin hors
87 and enzyme modified Au nanoparticles to the biotinylated detection probe and the subsequent injectio
88 on in rats by injecting anterograde tracers, biotinylated dextran amine (BDA) and Phaseolus vulgaris-
89 onally, inefficient extrinsic tracers such a biotinylated dextran amine (BDA) are used to label regen
91 gle injections of the neuroanatomical tracer biotinylated dextran amine (BDA) into different tonotopi
98 the zebra finch, using in vivo injections of biotinylated dextran amine and verification of projectio
99 ctions to "rUva" resulted from injections of biotinylated dextran amine into the lateral pontine nucl
104 h the anterograde tracers (3) H-amino acids, biotinylated dextran amine, and Phaseolus vulgaris leuco
105 ticle-conjugated cholera toxin B-subunit and biotinylated dextran amine, were placed in subdivisions
106 C57BL/6 mice were injected unilaterally with biotinylated dextran into thoracolumbar DRG (T13-L3).
107 ied utilizing anterograde tract-tracing with biotinylated dextranamine (BDA) and fluorescence immunoh
108 ojections of the ACo by using fluorogold and biotinylated dextranamines as retrograde and anterograde
110 As an application of this recovery method, biotinylated DNA fragments are isolated from a mixed sol
112 agnetic-MIPs for the detection of biotin and biotinylated DNA in magneto-actuated platforms is also d
114 usion remodeler, we found that targeting via biotinylated DNA resulted in directional sliding towards
116 ed (CHA) probes, AS1411 aptamer, and pendent biotinylated DNA strand in different vertexes and is fur
118 her rallied an in situ interdigital relay of biotinylated DNA(TTalpha/beta) into a microsized hyperli
119 unwinding, displacement of streptavidin from biotinylated DNA, translocation on single-stranded DNA (
121 DNA onto a paper membrane, and addition of a biotinylated-DNA detection probe, hybridization was evid
122 l as quantitative suspension array assays of biotinylated-DNA functionalized upconversion nanoparticl
124 l growth factor (EGF) receptors (EGFR) using biotinylated EGF bound to streptavidin that is covalentl
125 to chicken egg white lysozyme, in which one biotinylated electrochemistry (EC)-cleaved peptide was i
131 rial allowed the anchoring by bioaffinity of biotinylated glucose oxidase (biot-GOx) as proof-of-conc
133 idin-modified MPs, which were conjugated via biotinylated glycan (through streptavidin-biotin affinit
134 In addition, we show that cross-linking of biotinylated HA in streptavidin multimers or supramolecu
135 the recruitment site, whereas targeting via biotinylated histones produced a distribution of nucleos
136 ns were incubated with anti-Nrf2 primary and biotinylated-horseradish peroxidase-conjugated secondary
137 elomerase RNA component and the N-terminally biotinylated human telomerase reverse transcriptase and
140 trast, the germination proteins were largely biotinylated in germinated spores, although GFP was not.
141 original JBP1, this new recombinant JBP1 was biotinylated in vivo and conjugated to magnetic beads vi
143 entified over 50 cellular proteins that were biotinylated, including membrane and cytoskeletal protei
145 se reporter assay and a pulldown assay using biotinylated INS-class I VNTR probe were performed to ex
146 -(12-mercaptododecyl)-imidazolium bromide, a biotinylated ionic liquid (IL-biotin), which self-assemb
147 st protein dictates the configuration of the biotinylated Ir-cofactor which, in turn, by and large de
148 ne reductase based on the incorporation of a biotinylated iridium pianostool complex within streptavi
149 ylated activated factor XII (factor XIIa) or biotinylated kallikrein bound to avidin-coated plates.
152 GD-peptides' ability to inhibit binding of a biotinylated "knottin"-RGD peptide to surface-immobilize
154 ly targeted using CPS carrying two different biotinylated ligands, HaloTag substrates or anti-GFP nan
155 lowed by reversal of cross-links, capture of biotinylated ligation junctions on streptavidin-coated m
157 x was bound to a supported lipid bilayer via biotinylated lipid streptavidin complexes (pinning sites
161 The affinity of avidin for biotin allows biotinylated liposomes to complex in the presence of avi
165 Here we determine lncRNA localization using biotinylated locked nucleic acid (LNA)-containing oligon
166 were used in lateral flow assays (LFAs) with biotinylated lysozyme as a model analyte in buffer and m
167 ptor in the synaptic plasma membrane and the biotinylated M1 protein associated with the total plasma
169 ific bacterial protein streptavidin binds to biotinylated macromolecules like proteins, antibodies, o
171 dox sensitivity changes were confirmed using biotinylated methyl-3-nitro-4-(piperidin-1-ylsulfonyl) b
173 enes, we isolated mRNAs bound to transfected biotinylated miR-200c in mouse breast cancer cells.
175 er, the ability of streptavidin to bind four biotinylated molecules in a heterogeneous manner is ofte
178 under magnetic actuation of a wide range of biotinylated molecules, and their downstream application
179 wed a significant immobilization capacity of biotinylated molecules, giving rise to a cheaper and a r
181 form based on streptavidin coated tips and a biotinylated mouse anti-hIgG capture antibody targeting
182 was effectively displayed on the surface of biotinylated mouse islets without a negative impact isle
185 The key element is a site-specific in vivo biotinylated nanobody of broad cross-reactivity with mic
186 in, with 6- to 18-fold higher uptake vs. non-biotinylated nanoparticle and fusion protein controls.
187 iple target cells by measuring the uptake of biotinylated nanoparticles by lymphoma cells with distin
191 with observations that BirA*-tagged proteins biotinylate neighbor proteins that are in close proximit
192 upts the interaction of endogenous Mcl-1 and biotinylated Noxa-BH3 peptide, causes cell death through
195 ent a variation of Structure-seq2 in which a biotinylated nucleotide is incorporated during reverse t
196 ilylidene-2-azido-2-deoxy-selenogalactoside, biotinylated oligo-alpha-(1 -> 4)-d-galactosamines compr
199 d on sequential rounds of hybridization with biotinylated oligonucleotides that enables more than 1-m
200 ized pCBAA with target miRNA in EL (bound to biotinylated oligonucleotides) and (ii) the capture of s
201 endogenous iRhom1 and -2 can be cell surface-biotinylated on mouse embryonic fibroblasts (mEFs), reve
202 efficiently with fluorescent, spin-labeled, biotinylated, or cross-linker-modified guanosine triphos
206 ntibodies enable unprecedented enrichment of biotinylated peptides from complex peptide mixtures.
208 trategy to identify both nonbiotinylated and biotinylated peptides in a single experimental setup.
214 tionalized with self-assembled monolayers of biotinylated polyethylene glycol thiols, neutravidin and
215 olves deposition by electropolymerization of biotinylated polythiophene films on the surface of minia
217 with LAMP reagents including the analyte and biotinylated primers, and their hydrodynamic volumes are
220 (SAMs) was used for immobilized streptavidin-biotinylated probes on the sensor surface for the detect
224 labeling proteomics data to the endogenously biotinylated protein (PCCA) can greatly reduce variation
225 ELISA-like assay using, in pilot tests, the biotinylated protein and HRP-labeled streptavidin for it
226 vealed that Pyc is the most rapidly depleted biotinylated protein and metabolomics revealed a broad m
229 efly, we demonstrate that small amounts of a biotinylated protein of interest can be used to generate
230 Quantitation of the kinetics of depletion of biotinylated proteins after biotin withdrawal revealed t
231 ist, such as interferences from endogenously biotinylated proteins and other highly abundant bystande
234 dress this, we used affinity purification of biotinylated proteins expressed transiently in Nicotiana
237 Our experiments reveal a diverse set of biotinylated proteins not previously reported at the AIS
239 Localization patterns of PrGag proteins and biotinylated proteins overlapped, consistent with observ
240 ication-mass spectrometry (AP-MS) identified biotinylated proteins, which were analyzed for statistic
242 ring the layer-by-layer sensor construction, biotinylated PrP(C) (95-110) was attached via a biotin/N
244 ombination with the broad option of existing biotinylated reagents offering a great versatility for t
245 l-time detection of donkey meat marker using biotinylated reporter and streptavidin functionalized go
246 r D-dimer a two-site immunoassay employing a biotinylated reporter antibody and reaction with strepta
247 This uses a sandwich assay strategy in which biotinylated reporter probes recognize TDN-bound target
251 , the folded aptamer was able to bind to the biotinylated RNA hairpin module through loop-loop intera
252 mpetitive assay between the m6A target and a biotinylated RNA oligomer bearing a single m6A enzymatic
253 ucleoprotein complex immunoprecipitation and biotinylated RNA pull-down assays demonstrated that PARP
257 we synthesized two sets of monodispersed and biotinylated [Ru(bpy)(3) ](2+) -doped silica nanoparticl
258 sely high and results from 4 out of 15 (27%) biotinylated sandwich immunoassays tested falsely low.
259 streptavidin-linked antibodies conjugated to biotinylated saporin, a toxin unable to cross cell membr
261 tection of the bound bacterial toxins with a biotinylated secondary antibodies and streptavidin-coate
262 achieved when captured analytes labeled with biotinylated secondary antibodies, which then capture st
265 rom biological samples by hybridization with biotinylated sense-strand oligonucleotides coupled to st
268 ere immobilized on cellulose paper, and then biotinylated single-stranded oligonucleotide probes were
271 thod by monitoring binding of NeutrAvidin to biotinylated spheres and binding of immunoglobulin G to
273 In the developed assay, the enzyme-specific biotinylated substrate peptide is post-translationally m
274 in-streptavidin interaction are bound to the biotinylated surface of the target cell, and anti-IL-22
277 e find that divalent interaction of SAv with biotinylated surfaces is a strict requirement for stable
278 streptavidin (SAv) binding to commonly used biotinylated surfaces such as supported lipid bilayers (
281 by successive rounds of biopanning against a biotinylated synthetic peptide comprising repetitive imm
282 ane proteins carrying a short, enzymatically biotinylated tag, compatible with SRI techniques includi
283 ry oligonucleotide probe hybridized with the biotinylated target oligonucleotide followed by reaction
284 receptor Lrp6 was fused to APEX2 and used to biotinylate targets that are recruited near the receptor
285 ous assay) format using His-tagged pTEFb and biotinylated Tat to monitor the interaction between Tat
286 of avidin, as a non-covalent complex with a biotinylated Tat vector, is also efficient but the prote
291 ts indicate that BirA*-tagged PrGag proteins biotinylated themselves as well as WT PrGag proteins in
293 ntibody-modified magnetic beads (Ab-MBs) and biotinylated thiol-DNA gold nanoparticles (biotin-GNPs)
296 the TRbeta1 cistrome in vivo, we expressed a biotinylated TRbeta1 in hypo- and hyperthyroid mouse liv
297 cal synthesis of all-l- and all-d-amino acid biotinylated variants of oncogenic mutant KRas(G12V).