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1 gy for large-scale mutational analysis (RESA-bisulfite).
2 ver, we demonstrate that Glut-3SH-Al and its bisulfite adduct are present in grape juice and could be
6 ide applicability and can be extended to the bisulfite adducts of common warheads employed in the des
7 on and stereochemistries of all six isomeric bisulfite adducts to Psi; conversion of intermediate add
12 by parallel processing of genomic DNA using bisulfite and oxidative bisulfite conversion in conjunct
14 xBS-MLE based on binomial modeling of paired bisulfite and oxidative bisulfite data from sequencing o
16 the reactions of sulfite (sulfite anions or bisulfite anions) with holes or hydroxyl radicals are th
18 ed a strong agreement between S9.6-based and bisulfite-based R-loop mapping and confirmed that R-loop
20 age of the method, comprising cell lysis and bisulfite (BS) conversion, preamplification and adaptor
23 -glutathionyl caftaric acid, suggesting that bisulfite consistently competes as a nucleophile with gl
24 ine to support the comprehensive analysis of bisulfite conversion and immunoprecipitation-based methy
25 ther with experimental parameters, including bisulfite conversion and oxidation efficiencies, as well
26 We also provide a bead-based protocol for bisulfite conversion and size selection of DNA and RNA f
27 Using methylation-sensitive restriction and bisulfite conversion assays, we further showed that PSTV
29 of genomic DNA using bisulfite and oxidative bisulfite conversion in conjunction with RNA sequencing.
30 easured at the single CpG level using sodium bisulfite conversion of genomic DNA followed by sequenci
32 ut sequencing of cell-free DNA (cfDNA) after bisulfite conversion to map the tissue and cell types of
33 Gold-standard, widely used technologies (bisulfite conversion, followed by deep sequencing) canno
34 ATAC-seq or ChIP-seq (M-ATAC or M-ChIP) with bisulfite conversion, to simultaneously examine accessib
36 d amplicon, massively parallel sequencing of bisulfite converted DNA in a compact and interpretable f
38 ing reads from high-throughput sequencing of bisulfite-converted DNA to reconstruct heterogeneous cel
39 genetic variation and DNA methylation using bisulfite-converted DNA, and unlocks the use of personal
40 VAliBS provides fast and accurate mapping of bisulfite-converted reads, and a friendly window system
43 ges from the C nucleoside Psi to form stable bisulfite end products that yield signatures for next-ge
45 essibility Protocol for individual templates-Bisulfite Genome Sequencing (MAPit-BGS) and nucleosome o
46 methylation of five genes was quantified by bisulfite genomic sequencing in d-200 dorsal prostates a
49 -(hexanal)-glutathione (Glut-3SH-Al) and its bisulfite (Glut-3SH-SO3) adduct were identified in Sauvi
50 lve the treatment of genomic DNA with sodium bisulfite; however, this method cannot distinguish betwe
51 ith Psi at a known location was treated with bisulfite, leading to a deletion signature after reverse
52 unoprecipitation and methylation assays, and bisulfite mapping, we demonstrate that human methyltrans
54 s within regulatory elements, we developed a bisulfite-mediated nucleotide-conversion strategy for la
56 son with values obtained from an established bisulfite next-generation sequencing approach for determ
59 lated by HPLC, diluted in pH 1-9, mixed with bisulfite or ascorbic acid at pH 3, and stored for 8 wee
60 en demonstrated that paired BS and oxidative bisulfite (oxBS) treatment on the same sample followed b
63 a or serum of donors, treated the cfDNA with bisulfite, PCR-amplified the cfDNA, and sequenced it to
64 robiol 71:3131-3136, 2005) and one utilizing bisulfite pretreatment of DNA to reduce variation prior
66 for DNA methylation and mRNA levels by using bisulfite pyrosequencing and quantitative RT-PCR in mono
69 owed that MeTIL markers can be determined by bisulfite pyrosequencing of small amounts of DNA from fo
71 uantitative PCR), DNA methylation status (by bisulfite pyrosequencing), and GAL peptide by RIA of the
72 digest and ten-eleven translocation-assisted bisulfite pyrosequencing, to quantify FMR1 5mC and 5hmC
73 ics analyses, multicolor flow cytometry, and bisulfite pyrosequencing, to study the effects of H pylo
77 AT_MM, of which the significant retention of bisulfite-resistant cytosines was corroborated by reanal
80 ed, methods to assess Treg stability such as bisulfite Sanger sequencing to determine the methylation
84 tially active gene regulatory sequences; and bisulfite sequencing (BS-seq) quantifies DNA methylation
87 methylation signals with similar accuracy as bisulfite sequencing (BS-Seq; single nucleotide resoluti
88 we present customised Reduced Representation Bisulfite Sequencing (cuRRBS), a novel and easy-to-use c
90 dy, we describe low-input methylase-assisted bisulfite sequencing (liMAB-seq ) and single-cell MAB-se
91 have recently developed a methylase-assisted bisulfite sequencing (MAB-seq) method that allows base-r
92 Study (MACHS) were profiled by whole-genome bisulfite sequencing (median coverage: [Formula: see tex
93 deoxyuridine labeled nascent DNA followed by bisulfite sequencing (Repli-BS) measures post-replicatio
94 te sequencing (WGBS), reduced representation bisulfite sequencing (RRBS) and hybrid selection (captur
95 g was performed using reduced representation bisulfite sequencing (RRBS) and RNA-sequencing (RNA-Seq)
96 e apply our method to reduced representation bisulfite sequencing (RRBS) data from multiple regions o
98 raditional MspI-based Reduced Representation Bisulfite Sequencing (RRBS) protocol to all restriction
99 could be queried in a reduced representation bisulfite sequencing (RRBS) study of adult [Formula: see
100 We initially used reduced representation bisulfite sequencing (RRBS) to generate a methylome-wide
102 and 450 K arrays) and reduced representation bisulfite sequencing (RRBS), only cover a small proporti
103 lation differences by reduced representation bisulfite sequencing (RRBS), we determined that, over ti
105 Here, we employed an enhanced version of bisulfite sequencing (RRBS/oxRRBS) followed by next gene
106 me maps from single cells, using single-cell bisulfite sequencing (scBS-seq), allowing the quantitati
107 ture with single-cell reduced representation bisulfite sequencing (scRRBS), to measure the CpG methyl
108 tes sequencing (DNase-seq), and whole-genome bisulfite sequencing (WGBS or BS-seq) data revealed inte
109 hylation patterns comparable to whole-genome bisulfite sequencing (WGBS) along genes and regulatory e
113 ole-genome sequencing (WGS) and whole-genome bisulfite sequencing (WGBS) data on populations of moder
114 for detecting such events from whole-genome bisulfite sequencing (WGBS) data perform statistically i
115 oach to methylation analysis of whole genome bisulfite sequencing (WGBS) data permitted a high level
116 igenetic energy landscapes from whole-genome bisulfite sequencing (WGBS) data that enable us to quant
117 tematic comparisons of GPS with whole-genome bisulfite sequencing (WGBS) found that methylation diffe
122 m DNA methylation, we performed whole genome bisulfite sequencing (WGBS) on an equimolar pool of sper
125 increased disease risk we used whole genome bisulfite sequencing (WGBS) to analyze changes in DNA me
127 Pipelines are provided for whole genome bisulfite sequencing (WGBS), reduced representation bisu
133 ecipitation, luciferase promoter assays, and bisulfite sequencing analysis of sites in proximity.
136 enetic mark, was investigated using targeted bisulfite sequencing and characterized at functional lev
137 elopment in NeuN+ neurons using whole-genome bisulfite sequencing and compare them to non-neurons (pr
138 genome-wide, we used reduced representation bisulfite sequencing and found an extensive reorganizati
141 ation measurements confirmed by whole genome bisulfite sequencing and offers a better balance between
143 bisulfite sequencing, reduced representation bisulfite sequencing and RNA sequencing were performed o
144 re investigated using reduced representation bisulfite sequencing and RNA sequencing, respectively.
147 examine promoter accessibility, we performed bisulfite sequencing and show that none of the MHC class
150 everal cost-effective reduced representation bisulfite sequencing approaches (RRBS) have been recentl
151 nt a method to map DNA methylation data from bisulfite sequencing approaches to CpG sites measured wi
152 lation modifications from any combination of bisulfite sequencing approaches, including reduced, oxid
154 ulfite sequencing and reduced representation bisulfite sequencing brings the availability of DNA meth
155 HSD11B2 and FKBP5 are seen in a minority of bisulfite sequencing clones, these epigenetic changes, a
159 GE properly accounts for the count nature of bisulfite sequencing data and incorporates allele-specif
160 terns with those from Reduced Representation Bisulfite Sequencing data and through comparison with pr
161 we reanalyzed K562 RNA-seq and whole-genome bisulfite sequencing data for allele-specific expression
163 I 0.91-0.98) from the reduced representation bisulfite sequencing data from 35 whole blood samples.
166 ute deviations support the validity of using bisulfite sequencing data in combination with Illumina b
168 d validation with 101 reduced-representation bisulfite sequencing data sets and 637 methylation array
169 pe blocks, after analysis of 61 whole-genome bisulfite sequencing data sets and validation with 101 r
170 ion sequencing (MeDIP-seq), and whole-genome bisulfite sequencing data to generate and orthogonally v
171 tus and DNA polymorphisms, from whole-genome bisulfite sequencing data, and nucleosome occupancy from
172 dreds of transcription factors, whole-genome bisulfite sequencing data, ChIA-PET data, and functional
173 Using high resolution hairpin oxidative bisulfite sequencing data, we precisely determine the am
176 o increase coverage of existing whole genome bisulfite sequencing datasets by imputing CpG methylatio
177 re, we have analyzed 34 diverse whole-genome bisulfite sequencing datasets in human and identified 31
178 nalysis of both human and mouse whole genome bisulfite sequencing datasets reveals read-level signatu
179 ally methylated regions from high-throughput bisulfite sequencing datasets, DMRfinder is the first th
180 present a set of preprocessing pipelines for bisulfite sequencing DNA methylation data that include a
185 e information from whole genome and targeted bisulfite sequencing from 910 samples to perform genotyp
190 We examined DNA methylation by whole-genome bisulfite sequencing in neuronal and non-neuronal popula
192 s, publicly available reduced representation bisulfite sequencing in the human embryo and germ cells,
193 cers and promoters, genome-wide, by targeted bisulfite sequencing in two independent sample cohorts.
196 by the Illumina 450K array and whole genome bisulfite sequencing is still too expensive for many sam
199 ying whole-exome sequencing and whole-genome bisulfite sequencing of cell free DNA (cfDNA) and of mat
201 methylation is measured by deep whole-genome bisulfite sequencing of genomic DNA from tissues represe
205 to leukemogenesis, we performed whole-genome bisulfite sequencing of primary leukemic and non-leukemi
206 By employing a protocol for whole-genome bisulfite sequencing of single cells, we show that the l
207 nalysis, and enhanced reduced representation bisulfite sequencing on a cohort of paired diagnosis and
208 in social insects, we performed whole-genome bisulfite sequencing on brains of the clonal raider ant
209 nd laser-capture microdissection followed by bisulfite sequencing on DNA isolated from prostate tissu
211 analyze DNA methylation data generated from bisulfite sequencing or Illumina methylation arrays.
214 g of short reads by and high cost of current bisulfite sequencing platforms make them impractical for
225 encing studies, including RNA sequencing and bisulfite sequencing studies, are becoming increasingly
227 we developed RBS-Seq, a modification of RNA bisulfite sequencing that enables the sensitive and simu
228 ltiplexed single-cell reduced-representation bisulfite sequencing to B cells from healthy donors and
231 microdissection with reduced representation bisulfite sequencing to identify cancer-associated DNA m
233 on, DeepSignal achieves 90% correlation with bisulfite sequencing using just 20x coverage of reads, w
234 g (n = 12 per group), unbiased capture array bisulfite sequencing was combined with subsequent matrix
235 lation and hydroxymethylation with oxidative bisulfite sequencing was conducted and correlated with c
244 RNA sequencing and reduced representation bisulfite sequencing were used to create transcriptomic
245 lated methods such as nasBS-seq (nascent DNA bisulfite sequencing), ChIP-BS-seq (ChIP followed by bis
246 e sequencing), ChIP-BS-seq (ChIP followed by bisulfite sequencing), TAB-seq, oxBS-seq, and fCAB-seq.
247 oprecipitation with sequencing, whole-genome bisulfite sequencing, and chromosome conformation captur
248 efficient (R = 0.884) between our method and bisulfite sequencing, and for 92.0% of CpG sites, methyl
249 epigenome, replication-timing, whole-genome bisulfite sequencing, and gene expression profiles from
250 ipitation-sequencing, reduced representative bisulfite sequencing, and RNA-sequencing were performed
251 ifications, including but not limited to RNA bisulfite sequencing, m(1)A-Seq, Par-CLIP, RIP-Seq, etc.
252 ular biology and optical approaches, such as bisulfite sequencing, microarrays, quantitative real-tim
253 biased omics profiling, such as whole genome bisulfite sequencing, reduced representation bisulfite s
255 onic stem cells and show that, compared with bisulfite sequencing, TAPS results in higher mapping rat
256 sign, particularly in reduced representation bisulfite sequencing, there is a need to develop more fl
259 e used whole-genome sequencing, whole-genome bisulfite sequencing, whole transcriptome (RNA-seq) and
260 ned using single cell reduced representation bisulfite sequencing, with a 66-fold increase in the fra
261 omplexes of DNMT3A and DNMT3B, and performed bisulfite sequencing-based single-turnover methylation a
273 genes was analyzed using 450K Beadchips and bisulfite sequencing; correlations between maternal and
278 dresses statistical challenges introduced by bisulfite-sequencing while controlling for complex sourc
281 tutional isomers of an S- and an O-adduct of bisulfite to the ribose, and these are the final product
284 affinity of guanine bases towards graphene, bisulfite-treated guanine-enriched methylated DNA leads
286 tional aligners are not designed for mapping bisulfite-treated reads, where the un-methylated 'C's ar
289 is studied at a single-base resolution with bisulfite treatment followed by high-throughput sequenci
290 ribe the isolation of genomic DNA (gDNA) and bisulfite treatment for the assessment of DNA methylatio
291 nature generated during cDNA synthesis after bisulfite treatment for which the chemical details of th
292 uencing (NGS) on plasma DNA with and without bisulfite treatment from mCRPC patients receiving either
294 -methylcytosine, and pseudouridine (Psi) via bisulfite treatment of RNA provides a key advance to loc
298 ngle-cell protocol based on agarose-embedded bisulfite treatment, which allows investigating DNA meth