ライフサイエンスコーパス: blinking

1 fer from intermittent on/off light emission (blinking).

2 cence known as fluorescence intermittency or blinking.

3 oteins and overcounting owing to fluorophore blinking.

4 hip between charge trapping and fluorescence blinking.

5 the more likely he or she will be to inhibit blinking.

6 s of single-dot emission intensity, known as blinking.

7 mission and the most complete suppression of blinking.

8 tinuously to track vertical movements during blinking.

9 vertical meridian was 342 +/- 155 mum after blinking.

10 e goal of clarifying the role of charging in blinking.

11 cules and quantum dots, without bleaching or blinking.

12 inked normally or successfully inhibited eye blinking.

13 ide robust signals without photobleaching or blinking.

14 tracts to close the palpebral fissure during blinking.

15 he interfacial ET reactivity fluctuation and blinking.

16 nking, but it becomes altered during delayed blinking.

17 nt constraint on possible mechanisms for the blinking.

18 long-lasting change in eyelid asymmetry with blinking.

19 k population does not arise from millisecond blinking.

20 ion, presenting intermittent luminescence by blinking.

21 e we address these questions, by focusing on blinking.

22 s heart rate, galvanic skin response and eye blinking.

23 e can also voluntarily blink or refrain from blinking.

24 the high excitation power required to induce blinking.

25 fluorophore interactions, as well as on-off blinking.

26 e measurements by accounting for fluorophore blinking.

27 e-aggregate emission characterized by strong blinking.

28 Blinking 5 times facilitated such recovery in normal sub

29 mically engineering ligand binding moieties, blinking accompanied by photodegradation still poses bar

30 facial affect, reduce eye motion, and reduce blinking, all in time with the rhythm of their singing a

31 fering from foreign body sensation, frequent blinking and bilateral inferior conjunctivochalasis was

32 Our Bayesian analysis of the blinking and bleaching (3B analysis) method models the e

33 Bayesian blinking and bleaching (3B) reconstruction reveals that

34 nifies the analysis of the localization from blinking and bleaching events.

35 of adenine and further confirmed by digital blinking and bleaching in the temporal domain.

36 Although blinking and characteristic emission patterns demonstrat

37 tacrolimus, and tacrolimus solutions trigger blinking and cold-evoked behaviors.

38 II electronic structures exhibit suppressed blinking and diminished nonradiative Auger recombination

39 rvations are subject to fluorophore multiple blinking and each protein is included in the dataset an

40 e intervention of such processes, as well as blinking and kindling, in fluorescent proteins.

41 ies, including bright, narrow-linewidth, non-blinking and non-bleaching emission in the second near-i

42 ing and oxidizing systems (ROXS) that reduce blinking and oxygen scavenging systems to reduce bleachi

43 both this multi-colour emission process, and blinking and photobleaching behaviours of single tetrapo

44 of these emitters are frequently degraded by blinking and photobleaching that arise from poorly passi

45 in their emission characteristics, including blinking and photobleaching that limit their utility and

46 unwanted photophysical properties, including blinking and photobleaching, which limit their overall e

47 om their limited photostability evidenced by blinking and photobleaching.

48 Surface defect-induced photoluminescence blinking and photodarkening are ubiquitous in lead halid

49 ein also uncovered surprises, especially the blinking and photoinduced recovery of emitters, which st

50 lecule SERS including spectral fluctuations, blinking and Raman signal being generated from only sele

51 excited states are thus required to minimize blinking and sensitization of singlet oxygen.

52 han expected by chance, they inhibited their blinking and shifted visual fixation differentially with

53 re, we introduce a kinetic model to describe blinking and show that Dendra2 photobleaches three times

54 Although the frequent erratic blinking and substantial dark (never radiant) fractions

55 light harvesting by controlled fluorescence blinking and suggest that any contribution of the minor

56 gating the neural basis of human spontaneous blinking and suggest that the spinal trigeminal complex

57 r-individual variability in the frequency of blinking and the majority of subjects not vocalising or

58 ined: (1) eye closure times, (2) spontaneous blinking, and (3) spontaneous and eye closure-triggered

59 ng reconstruction to the detected stochastic blinking, and achieved a spatial resolution of at least

60 responsible for long-range photoluminescence blinking, and are also mobile.

61 nce with fast on-off switching, long-lasting blinking, and bright single-molecule emission.

62 ty, their brightness, long lifetime, lack of blinking, and chemical stability make nanoparticle based

63 hemical and mechanical means evokes tearing, blinking, and pain.

64 methods is complicated by photodegradation, blinking, and the presence of natural organic material a

65 t energy transfer, anomalous single particle blinking, and twinkling phenomena associated with polaro

66 Pupil diameter and blinking are influenced by arousal state, as are hemodyn

67 s methods used to measure single nanocrystal blinking are introduced.

68 We find that two distinct types of blinking are possible: conventional (A-type) blinking du

69 rmittency in nanocrystal emission, that is, 'blinking', arising from the escape of either one or both

70 Called bleaching/blinking assisted localization microscopy (BaLM), the te

71 zation precision in some other bleaching- or blinking-assisted techniques.

72 try, these processes may underlie changes in blinking associated with facial palsy and may play a rol

73 ulations, we establish that pNPP-induced dye blinking at the ~10-ms timescale is responsible for the

74 localization microscopy (SMLM) relies on the blinking behavior of a fluorophore, which is the stochas

75 intensity fluctuations that result from the blinking behavior of fluorophores, it is limited to case

76 We further found that the blinking behavior of mEos3.2 and mMaple3 is modified by

77 py but shows improvements in the fluorophore blinking behavior relative to the whole-mount approaches

78 -green fluorescence protein (GFP) revealed a blinking behavior similar to that reported for GFP-clath

79 long standing theories on photoluminescence blinking behavior.

80 mic diameter less than 11.0 nm), and reduced blinking behavior.

81 ed by suppression of both photobleaching and blinking behavior.

82 al days, and they exhibit markedly different blinking behavior; >20% of the g-NQDs do not blink, whil

83 hnique relies on the intrinsic bleaching and blinking behaviors characteristic of all commonly used f

84 pes of defect-induced photoluminescence (PL) blinking behaviors observed in single epitaxial InGaAs q

85 and Dendra2-T69A, we completely swapped the blinking behaviors of mEos2 and Dendra2, two popular PCF

86 ates, resulting in widely different apparent blinking behaviors that largely modulate the efficiency

87 nce trajectories show strong fluctuation and blinking between bright and dark states.

88 link entrainment, a temporal coordination of blinking between social partners engaged in dyadic inter

89 he statistical distribution of the number of blinking/binding events per emitter and infer the number

90 super-resolution methods rely on stochastic blinking/binding events, which often occur multiple time

91 d combination of localizations from multiple blinking/binding events.

92 ground and the background (i.e., fluorophore blinking, bleaching, or moving).

93 ically balanced and consistent during normal blinking, but it becomes altered during delayed blinking

94 have extended the measurement of quantum-dot blinking by characterizing fluctuations in the fluoresce

95 the earliest limbed vertebrates, suggesting blinking capability.

96 distribution correction (DDC), to eliminate blinking-caused repeat localizations without any additio

97 Postexternal DCR eyelid blinking changes included significant blink lagophthalmo

98 In contrast to fluorescent dyes that show blinking characteristics due to reversible photobleachin

99 8-channel wearable headset under controlled blinking conditions.

100 stimuli based on kinematic analyses of mouse blinking consistently suppress SbC-RGC spiking.

101 pparent/observed affinity to account for the blinking contribution.

102 t largely modulate the efficiency of current blinking correction procedures.

103 Blinking creates multiple localizations belonging to the

104 Vocalisations (d = 0.40), blinking (d = 0.37), and hiding (d = 0.37) were increase

105 ingle emitters directly from single-molecule blinking datasets, and therefore allows their locations

106 ults suggest that in macaques, as in humans, blinking depends not only on the physiological imperativ

107 tuations in the emission lifetimes (lifetime blinking), despite stable nonblinking emission intensity

108 age correlation spectroscopy of quantum dots blinking detects T cell receptor clusters on a scale of

109 NPs exhibit no on/off emission behavior, or "blinking," down to the millisecond timescale, and no los

110 atic oxygen-scavenging system eliminates Cy5 blinking, dramatically reduces photobleaching and improv

111 blinking are possible: conventional (A-type) blinking due to charging and discharging of the nanocrys

112 Here we show that unexpected blinking during graphene oxide-to-reduced graphene oxide

113 high blinking statistics and an appropriate blinking duty cycle on imaging quality, and developed a

114 Moreover, typical toddlers inhibited their blinking earlier than toddlers with ASD, indicating acti

115 onstrate effectively complete suppression of blinking even for long observation times of ~1 h.

116 standing photophysical properties: intrinsic blinking even in air, excellent fluorescence recovery, a

117 LC supports our proposal that each optical "blinking" event results from collision of a single supra

118 ividual dark and bright qdots and to observe blinking events as qdots freely diffused in aqueous solu

119 show that the distribution of the number of blinking events assumes a universal functional form, ind

120 ecular counting based on the distribution of blinking events from a single fluorophore.

121 cence enhancement of 480% and an increase in blinking events from a single spontaneously blinking flu

122 increase both the frequency and duration of blinking events of Cy5, an effect that scales with reduc

123 How and why did blinking evolve?

124 detailed mechanism is not fully understood, blinking experiments are found to provide direct evidenc

125 An overview of blinking experiments used to probe specific mechanisms f

126 e label comprising both fluorogenic and self-blinking features.

127 re fluorescence from rapid, sub-millisecond "blinking" fluctuations (fluid polymer environment) to co

128 ed emission (SPCE) substrates to enhance the blinking fluorescence of spontaneously blinking fluoroph

129 We found no evidence of blinking (fluorescence intermittency) in solution on nan

130 cy of a peptide labeled with a spontaneously blinking fluorophore contains information about the stru

131 blinking events from a single spontaneously blinking fluorophore; moreover, the required excitation

132 e the blinking fluorescence of spontaneously blinking fluorophores in single-molecule localization mi

133 Using spontaneous blinking fluorophores to label proteins of interest, we

134 ntly tagging single molecules with multiple, blinking fluorophores, the accuracy of the technique can

135 s of fluorescent beads or directly images of blinking fluorophores, the raw data in single-molecule l

136 We monitored spontaneous blinking for 55 min periods in normal conditions and aft

137 ntrinsic stochastic fluorescence emission or blinking from unstained polymers and performed spatial-t

138 Eye-blinking has emerged as a promising means of measuring v

139 Studies of the blink reflex and spontaneous blinking have provided useful neurophysiologic informati

140 single-dot emission intermittency (known as blinking) have been recognized as universal requirements

141 epletion, more channels start blinking, with blinking heights increasing over time, suggestive of slo

142 uitable for molecular quantification through blinking histogram analysis.

143 The temporal synchrony of blinking, however, increased in response to segments dep

144 Here we present a miniaturized analog of a blinking human eye to reverse engineer the complexity of

145 ients with blepharospasm (BSP) and increased blinking (IB).

146 in charged nanocrystals, with successful non-blinking implementations demonstrated in CdSe-CdS core-t

147 ve (dark) states that result in fluorescence blinking in a variety of timescales.

148 lthough we remain largely unaware of our eye-blinking in everyday situations, eye-blinks are inhibite

149 This study therefore examined blinking in freely-moving peacocks (Pavo cristatus) to d

150 and single-molecule microscopy to show that blinking in mEos2 and Dendra2 is largely controlled by t

151 responsible for the regulation of normal eye-blinking in mice.

152 Fluorescence blinking in nanocrystal quantum dots is known to exhibit

153 -domain (TD)-OCT before and while preventing blinking in order to produce a wide variety of signal st

154 e additional applicability toward explaining blinking in other systems, a problem of current interest

155 Whereas photoconversion and red-state blinking in PCFPs have been studied intensively, their g

156 blink amplitude and the rate of spontaneous blinking in rodents.

157 Our high-speed videos show needles blinking in slow motion in a sequential mode.

158 ibited a significant increase in spontaneous blinking in the light relative to the blink rate in dark

159 administration of a TRPM8 antagonist reduced blinking in wild-type mice.

160 that takes advantage of the intermittency ("blinking") in the fluorescence of quantum dots (QDs) to

161 on of a semi-involuntary motor behavior, eye blinking, in children and adults with Tourette syndrome

162 alysis of visual input signals, we show that blinking increases the power of retinal stimulation and

163 he ocular surface and resists clearance from blinking, increasing the intraocular absorption of hydro

164 and in blood volume dropped following awake blinking, indicating a reset of neural and vascular acti

165 ightness per particle, saturation intensity, blinking (intermittency), hydrodynamic radius, and prope

166 emporal fluctuations (caused by fluorescence blinking/intermittency) recorded in a sequence of images

167 f the normal pattern of episodes of frequent blinking interspersed with intervals having few blinks.

168 ugh which the basal ganglia modulates reflex blinking is (1) the substantia nigra pars reticulata inh

169 Because of its simplicity, blinking is a prominent model system in analysis of adap

170 Blinking is an effective compensatory mechanism to disti

171 Rapid blinking is associated with worse ocular surface disease

172 The observed single-molecule fast blinking is attributed to conversions between the fluore

173 stent with the predictions of models wherein blinking is controlled by diffusion of the energies of e

174 ting fluorophore photobleaching and unwanted blinking is crucial for single-molecule fluorescence (SM

175 Fluorescence intermittency or blinking is observed in nearly all nanoscale fluorophore

176 Furthermore, blinking is often spared in patients with severe brain i

177 Although spontaneous blinking is one of the most frequent human movements, li

178 trast with previous observations, single-dot blinking is significantly suppressed with only a relativ

179 After lid blinking, it took a longer time (2.2 +/- 1.1 second) to

180 roscopy (FCS) can resolve the intrinsic fast-blinking kinetics (FBKs) of fluorescent molecules that o

181 f the mechanisms responsible for nanocrystal blinking kinetics as well as core-shell engineering effo

182 Here, we evaluate the blinking kinetics of four photoactivatable fluorescent p

183 nts over existing dyes, and the nonpower law blinking kinetics suggest that these very small species

184 period may be required to confirm if eyelid blinking lagophthalmos and velocity will return to basel

185 ts, the volunteers were asked to fixate on a blinking LED.

186 However, eye tears and frequent blinking limit drug retention on the ocular surface, and

187 uctor nanostructures could share the same PL blinking mechanism.

188 Both blinking mechanisms can be electrochemically controlled

189 The development of numerous blinking mechanisms is reviewed, as is the physical natu

190 te over a decade of research, completely non-blinking nanocrystals have not been synthesized and an u

191 ntinuous output of single photons, these non-blinking nanocrystals may enable substantial advances in

192 Here we show that blinking nanorods (NRs) interact with each other in a cl

193 e STORM localization data based on their non-blinking nature and uses them as landmarks for channel r

194 s kept constant during blinks, we found that blinking nevertheless suppressed activity in visual cort

195 ymer environment) to completely "on" with no blinking observed (molecules adsorbed to a rigid bare gl

196 Incomplete blinking occurred significantly less often in women (51.

197 While quantum dot blinking occurs during these measurements, we describe a

198 Natural blinking occurs involuntarily, but one can also voluntar

199 tep with a slow transition that accounts for blinking of 527 nm emission at the single molecule level

200 Our approach allowed us to monitor fast blinking of an organic dye, the dissociation kinetics of

201 high-speed videography that recorded eyelid blinking of both eyes for a total duration of 4 minutes

202 Photoinduced blinking of Cy5 has hampered many previous investigation

203 Simple light-induced blinking of eYFP and collisional flux onto the cell surf

204 Photobleaching and blinking of fluorophores pose fundamental limitations on

205 plasmon resonance effects result in optical blinking of GNPs at a size-dependent wavelength.

206 istics due to reversible photobleaching, the blinking of GNPs seems to be stable for long periods of

207 Stochastic blinking of organic dyes in oxygen scavenging and thiol

208 The intrinsic blinking of photoactivatable fluorescent proteins mEos2

209 f qdots localized in dilute agarose gel, the blinking of qdots was measured across five orders of mag

210 However, the stochastic blinking of single fluorophores can introduce large unce

211 ecules is their photo-reactivity, leading to blinking of the fluorescence signal, and eventually to i

212 rora, a spectacular emission that appears as blinking of the upper atmosphere in the polar regions, i

213 alization micro-scopy studies of fluorophore blinking offer a promising route to probe oligomeric sta

214 ow dynamic music was associated with reduced blinking on double cue trials, suggesting greater cue-ba

215 -molecule emission rates, and essentially no blinking on experimentally relevant time scales (0.1 to

216 a promising tool for studying the impact of blinking on tear dynamics.

217 graphy (OCT) was used to study the impact of blinking on tear dynamics.

218 from purely mechanical or optical effects of blinking on visual input by combining pupil-independent

219 go repeated cycles of fluorescent emission ('blinking') on a timescale of several seconds-behaviour t

220 meniscus did not change significantly during blinking or during the open-eye period.

221 symmetric mouth elementary motor (p = 0.1), blinking (p = 0.05), and chewing/repetitive swallowing (

222 ng agent, offering the possibility to adjust blinking parameters according to experimental needs.

223 Temporal crossing of trajectories, blinking particles, and false-positive localizations pre

224 been synthesized and an understanding of the blinking phenomenon remains elusive.

225 uding suppressed fluorescence intermittency (blinking), photobleaching, and nonradiative Auger recomb

226 robenzyl alcohol, act to favorably attenuate blinking, photobleaching, and influence the rate of phot

227 ls that individually exhibit continuous, non-blinking photoluminescence.

228 al clustering analysis that leverages on the blinking photophysics of specific organic dyes showed th

229 Blinking, previously seen in confined zero- and one-dime

230 reas high dynamic listeners showed increased blinking probabilities, possibly reflecting internally g

231 ts but did increase the detected fraction of blinking qdots, suggesting that the dark population does

232 this method, time-lapse sequences of single blinking QDs were acquired and the centroids of the poin

233 Images of the blinking QDs were then overlapped in software, pixel by

234 Novel non-blinking quantum dots (NBQDs) were utilized in three-dim

235 Photoluminescence blinking--random switching between states of high (ON) a

236 ficantly (P = .02) greater lagophthalmos and blinking rate (P = .04).

237 A significantly higher blinking rate and lagophthalmos were found in subjects w

238 The blinking rate was also significantly greater in subjects

239 Blinking rate was linked to local river noise, aggressiv

240 ative correlation between song amplitude and blinking rate, consistent with a noise-driven multimodal

241 The blinking rate, eye closure, heart rate, alpha and beta b

242 Blinking rate, lagophthalmos, eyelid laxity, MGD, Schirm

243 However, the blinking rates can be too slow and nonuniform, which int

244 Despite efforts to achieve blinking reduction by chemical engineering of the QD arc

245 rial infections impairs tear production, the blinking reflex, and epithelial wound healing, resulting

246 to be awake after blinking than before, and blinking resets neural activity.

247 Spontaneous eye blinking serves a critical physiological function, but i

248 During normal and delayed blinking sessions, the tear film thickness increased sig

249 As photoluminescence blinking severely limits the usefulness of nanocrystals

250 tein-Debye relation and can be quantified by blinking signal.

251 phenethylammonium ligands exhibit nearly non-blinking single photon emission with high purity (~ 98%)

252 models conceptualize spatial attention as a blinking spotlight that sequentially samples visual spac

253 t is an intrinsic consequence of quantum dot blinking statistical ageing.

254 ned the effects of a high photon count, high blinking statistics and an appropriate blinking duty cyc

255 n a cluster, and the interactions affect the blinking statistics.

256 o determine molecule counts from fluorophore blinking statistics.

257 of the QD architecture and its environment, blinking still poses barriers to the application of QDs,

258 -down modulation of visual processing during blinking, suggesting a possible mechanism by which blink

259 g suppression, such that larger cores afford blinking-suppressed behavior at relatively thinner shell

260 ume (~750 nm(3)) that is required to observe blinking suppression and that this particle volume corre

261 anocrystals; however, the physics underlying blinking suppression remains unclear.

262 raction, quantum efficiency improvement, and blinking suppression through a photonic crystal (PC) sur

263 orrelation between g-NQD particle volume and blinking suppression, such that larger cores afford blin

264 hereas ZnSe/CdS gQDs show characteristic gQD blinking suppression, though only if shelling is accompa

265 thicker shells to realize the same level of blinking suppression.

266 Plasmonic hotspots generate a blinking Surface Enhanced Raman Spectroscopy (SERS) effe

267 shape is diagnostic of defects that control blinking, surface carrier dynamics, and other important

268 Mice were more likely to be awake after blinking than before, and blinking resets neural activit

269 xisting with DED exhibited more frequent eye blinking than that of patients with asymptomatic MGD (8.

270 d by significant fluorescence intermittency (blinking) that hinders applications as single-photon lig

271 In the first type of PL blinking, the "off" period is caused by the trapping of

272 or the "off" period in the second type of PL blinking, the electrons relax from the first excited sta

273 vestigate asymmetry in eyelid movements with blinking, the stability of the asymmetry, and its modifi

274 Blinking, the transient occlusion of the eye by one or m

275 We attribute B-type blinking to charge fluctuations in the electron-acceptin

276 compete with numerous other processes, from blinking to day-dreaming.

277 choline (DLPC), we measured the frequency of blinking to decrease proportionally with the number dens

278 We developed a rat model of spontaneous blinking to identify and better characterize the spontan

279 tion rates, extracted from photoluminescence blinking traces, showed a systematic increase from isola

280 deterministic all-optical suppression of QD blinking using a compound technique of visible and mid-i

281 nd photoswitching, (iii) phototoxicity, (iv) blinking, (v) permanent bleaching, and (vi) formation of

282 ilitate future engineering of bright and low-blinking variants suitable for PALM.

283 For each blink, lagophthalmos and eyelid blinking velocity were calculated for the operated eye,

284 Blinking was associated with increases in arousal and de

285 an rats, the temporal pattern of spontaneous blinking was qualitatively similar for both species.

286 Signal loss because of blinking was the most common artifact on 3D scans (optic

287 The tear meniscus height, with and without blinking, was recorded and calculated by video meniscome

288 with corrections for submillisecond acceptor blinking, we show that it is possible to obtain structur

289 parameters, lipid layer thickness (LLT) and blinking were compared among patients with asymptomatic

290 lower tear menisci during normal and delayed blinking were obtained from OCT images using custom soft

291 d with enhanced mutual gaze and empathic eye blinking, whereas indifference or malevolence was associ

292 d by large intensity fluctuations, known as 'blinking', whereby their photoluminescence turns 'on' an

293 ophthalmos with decreased velocity of eyelid blinking which gradually improved over the 3-month follo

294 ymer dots (Pdots), which exhibit spontaneous blinking with <5 nm localization error and a broad range

295 sistent temporal organization to spontaneous blinking with a median 750 s period that was independent

296 hough there are changes in the kinematics of blinking with age, such changes do not necessarily predi

297 In addition, they use conspicuous blinking with white-feathered eyelids to compensate for

298 Upon cAMP depletion, more channels start blinking, with blinking heights increasing over time, su

299 e music predicted non-linear fluctuations in blinking, with high dynamic music eliciting early blink-

300 surface showed fluorescence fluctuations and blinking, with time constants distributed from milliseco