ライフサイエンスコーパス: blotting

1 horylation (Na(V)1.5, at serine 571, Western blotting).

2 confirmed experimentally using tRNA Northern blotting.

3 -) mice after sepsis was assessed by Western blotting.

4 ransmission electron microscopy, and Western blotting.

5 lection fluorescence microscopy; and western blotting.

6 mple was taken to measure LTL using southern blotting.

7 borated by N-terminal sequencing and Western blotting.

8 ecipitated rOATP1A4 as determined by western blotting.

9 centration were measured by qPCR and western blotting.

10 in the rinse samples was analyzed by Western blotting.

11 ysis, density gradient analysis, and Western blotting.

12 time quantitative PCR (RT-qPCR) and Northern blotting.

13 mpulsive behaviours was confirmed on western blotting.

14 ein 2, c isoform (MAP2c) proteins by western blotting.

15 DLBCL cells were verified using RIP-Northern blotting.

16 l telomere length was assayed using Southern blotting.

17 sfected HK-2 cells was determined by western blotting.

18 chemokine gradients was supported by Western blotting.

19 s further confirmed by both FRET and western blotting.

20 2 and Bax, respectively, revealed by Western blotting.

21 rs were measured by qPCR, ELISA, and Western blotting.

22 0A antibody immunohistochemistry and Western blotting.

23 ood-stage parasites was confirmed by western blotting.

24 activation of NR4A1 was analyzed by western blotting.

25 e selected for further validation by western blotting.

26 vented seroconversion, as shown with western blotting.

27 ein level by confocal microscopy and western blotting.

28 P) receptor expression determined by Western blotting.

29 phosphorylation was determined using Western blotting.

30 mass spectrometry (MS) or individual western blotting.

31 ptor 4 (TLR-4) protein expression by Western blotting.

32 osphorylation of Chk1 and H2AX using Western blotting.

33 ation was analysed, and confirmed by Western blotting.

34 es, as measured by real-time PCR and western blotting.

35 ADAMTS13 antibody was detected by Western blotting.

36 ix (LYPAL), by mass spectrometry and Western blotting.

37 detected by immunohistochemistry and western blotting.

38 tative polymerase chain reaction and Western blotting.

39 measured by immunohistochemistry and Western blotting.

40 um samples was detected by ELISA and Western blotting.

41 ctions by co-immunoprecipitation and Western blotting.

42 acid (HOCl)-induced SSc by ELISA and Western blotting.

43 ng a more quantitative read-out than Western blotting.

44 ve reaction for platelet factor-4 by Western blotting.

45 4, and 6 (MVM, BM) was determined by Western blotting.

46 fers functionality to TLR4-levels by Western blotting.

47 Several changes can take place in wine after blotting.

48 H) were assessed by respirometry and Western blotting.

49 bility, electrophoretic mobility shifts, and blotting.

50 ex and putamen were determined using western blotting.

51 s were independently validated using Western Blotting.

52 A (VEGF-A) were analyzed by means of Western blotting.

53 tors, was evaluated by Sambucus nigra lectin blotting.

54 levels in Sprague-Dawley rats using western blotting, [(3)H]UCB-J autoradiography and immunostaining

55 P2X7R (HEK293-hP2X7R) and underwent Western blotting, an immunofluorescence assay, and radioimmunohi

56 Western blotting analyses revealed that 12(S)-HETE-lysophospholi

57 e transcriptase quantitative PCR and western blotting analyses, we confirm the direct regulation of C

58 structural, immunohistochemical, and Western blotting analyses, we found that human and mouse amnion

59 Western blotting analysis detected hot spots on 67-kDa proteins

60 microarray expression profiling and Western blotting analysis identified preferential phosphorylatio

61 Western blotting analysis of PELP1-cyto HMECs showed up-regulati

62 Western blotting analysis revealed a rapid decline in nuclear an

63 A Western blotting analysis revealed that biotin-labeled beauverio

64 re, using whole-cell patch clamp and Western blotting analysis, we demonstrate that the hERG/IKr chan

65 time quantitative PCR (RT-qPCR), and Western blotting analysis.

66 nhanced Cav2.3 protein expression by western blotting and abnormally large R currents in whole-cell v

67 3 (LC3), an autophagosome marker, by Western blotting and confocal fluorescence microscopy.

68 ophysiological characterization, and Western blotting and confocal imaging to assay expression and su

69 Moreover, western blotting and confocal microscopy revealed that LH stimul

70 in tumor cells by 50% as measured by Western blotting and flow cytometry.

71 Northern blotting and fluorescence microscopy indicate that ORFX

72 , florescence in situ hybridization, Western blotting and GTPgS autoradiography an upregulation of ex

73 dies and analyzed via Iba1 and P2X7R Western blotting and Iba1 immunohistochemistry.

74 acrophages or monocytes as judged by Western blotting and immunocytochemistry assays.

75 Western blotting and immunofluorescence analysis demonstrated ne

76 Here we use western blotting and immunofluorescence to examine the effects o

77 Western blotting and immunofluorescence were used for evaluating

78 d primary human hepatocytes (PHH) by Western blotting and immunofluorescence.

79 re reduced by IL-15, as indicated by Western blotting and immunofluorescent staining.

80 HASMCs) was evaluated using qRT-PCR, western blotting and immunofluorescent staining.

81 was determined by ELISA, zymography, Western blotting and immunofluorescent staining.

82 Trx1 levels as reflected by RT-PCR, Western blotting and immunohistochemistry analyses.

83 cells using a SMAD luciferase assay, Western blotting and Immunohistochemistry.

84 ial distribution were analyzed using Western blotting and immunohistochemistry.

85 Western blotting and immunostaining analysis confirmed that ShcA

86 ut of HEK 293 and A549 cells by both Western blotting and lipid mass spectrometry, we observed dimini

87 on of carbon sources, enzyme assays, Western blotting and mass spectrometric analysis to monitor and

88 Our Western blotting and messenger RNA expression data demonstrated

89 Western blotting and molecular docking studies suggested that th

90 Western blotting and MS results indicated that rHBeAg and HBeAg

91 performance leads to variability in Western blotting and other immunoassays.

92 Western blotting and PCR were used to assay changes in EC coupli

93 Established analytical methods like Western blotting and proteomics fail to detect the plethora of a

94 Western blotting and quantitative PCR analysis of infected cells

95 ere analyzed by immunosorbent assay, Western blotting and quantitative PCR.

96 esponding to ECM were measured using Western blotting and quantitative RT-PCR with subsequent matrix

97 Western blotting and Real-time PCR were used to assess the expre

98 abnormal prion protein (PrP(Sc)) by western blotting and real-time quaking-induced conversion (RT-Qu

99 ntioxidant system was carried out by Western blotting and showed sex-related differences.

100 n levels of the targeted proteins by Western blotting and used quantitative microscopic assays to con

101 ciferase assays, immunofluorescence, western blotting and virus infection.

102 LSE samples (quantitative RT-PCR and Western blotting) and in normal and AE skin biopsy specimens (im

103 measurements at both the cell population (by blotting) and single-cell (by imaging) levels.

104 ethods such as immunohistochemistry, western-blotting, and also by enzyme-linked immunosorbent assay.

105 uantitative PCR, immunofluorescence, Western blotting, and both chromogenic and single-molecule in si

106 by histology, immunohistochemistry, Western blotting, and chromatin immunoprecipitation (ChIP).

107 gation, immunoprecipitation, dot and Western blotting, and confocal imaging were performed for signal

108 lls was monitored by flow cytometry, Western blotting, and confocal/electron microscopy.

109 enomic RNA strands were detected by Northern blotting, and crystalline lattices of viral particles of

110 ioenergetics utilizing trypan blue, Southern blotting, and extracellular flux analysis, respectively.

111 ve electron microscopic morphometry, Western blotting, and functional tests.

112 termined by luminescence-based plate assays, blotting, and imaging.

113 s studied using immunoprecipitation, Western blotting, and immunofluorescence in NIH/3T3 cells transf

114 paracellular flux, mRNA expression, Western blotting, and immunofluorescence staining.

115 re assessed by qPCR, flow cytometry, Western blotting, and immunofluorescence.

116 cells was analyzed by real-time PCR, western blotting, and immunohistochemical staining.

117 evaluated by means of real-time PCR, Western blotting, and immunohistochemistry.

118 by fluoroenzyme immunoassay, immuno-Western blotting, and line-blot immunoassay.

119 re sampled for immunohistochemistry, western blotting, and mitochondrial high-resolution respirometry

120 physiological, immunohistochemistry, Western blotting, and patch clamping of membrane potentials was

121 cells were evaluated by using ELISA, Western blotting, and peptide epitope mapping.

122 by histology, immunohistochemistry, western blotting, and polymerase chain reaction.

123 In situ activity assays, Western blotting, and quantitative PCR were used to investigate

124 e assessed using immunofluorescence, Western blotting, and reverse transcriptase-polymerase chain rea

125 ar pathways was assessed by means of Western blotting, and the final outcome on immunomodulatory prop

126 scription polymerase chain reaction, Western blotting, and zymography.

127 podocytes using confocal microscopy; Western blotting; and adhesion and migration assays.

128 his hypothesis, we performed EYS Far-Western blotting assay and generated pomgnt1 mutant zebrafish.

129 In addition, H&E staining, TUNEL and Western blotting assays were performed in order to observed the

130 transcription-quantitative PCR, and Western blotting assessments showed that VSV-EBOVDeltaMLD produc

131 of M and S opsin were quantified by western blotting at 10 weeks.

132 LTL was measured by Southern blotting at baseline and ~14 yr thereafter.

133 MS-based protein identification and Northern blotting-based rRNA detection approaches identified two

134 reported mutations were compared by Western blotting, Ca2(+) flux assays, differentiation of transdu

135 analysis of proteins of interest by western blotting can be completed within 1-2 weeks.

136 roarray, quantitative real-time PCR, Western blotting, chromatin immunoprecipitation, and functional

137 Quantitative PCR (qRT-PCR) and Western blotting confirmed changes in expression of seven of the

138 coactivator of ACTA2 Two-dimensional Western blotting confirmed induction of both wild-type and mutan

139 lymerase chain reaction (RT-PCR) and Western blotting, confirming a concentration-dependent increase

140 erior affinity reagents suitable for western blotting, confocal fluorescence microscopy and pull-down

141 Size exclusion chromatography and Western blotting data obtained by using purified recombinant E1/

142 Western blotting demonstrated higher levels of inflammatory fact

143 olecular screening using qRT-PCR and Western blotting demonstrated that aldosterone treatment for 24

144 Western blotting demonstrated that Muller cell-specific AQP4 was

145 tive in all CJD PNS samples, whereas western blotting detected PrP(Sc) in the sciatic nerve in one VV

146 Western blotting determined P-gp and BCRP protein levels.

147 phosphorylation status in the GM by western blotting; DNA methylation by restriction digest and real

148 Using immunofluorescence assays and Western blotting during infection in cell culture and in chicken

149 When using northern blotting during these studies, we discovered that miRNA-

150 tion and DNA repair were analyzed by western blotting, electrophoretic mobility-shift assay, and immu

151 We used qRT-PCR, Western blotting, ELISA, and ChIP (chromatin immunoprecipitation

152 Northern blotting established that HCV dsRNA contained genome-len

153 roducible results within and between Western blotting experiments and the observed effect confirmed w

154 We corroborated by western blotting experiments that PTPN14 and CAV1 co-inmunopreci

155 Northern blotting experiments with rifampicin disclosed that the

156 ase binding competition assays, glycoprotein blotting experiments, and glycan array analysis employin

157 sing transfections of 293A cells and Western blotting experiments, we observed that RFFL and the core

158 ion and mRNA levels were analyzed by Western blotting, flow cytometry, and real-time PCR.

159 nd fibrosis were assessed by RT-PCR, western blotting, fluorescence-activated cell sorting (FACS), en

160 3b-II/LC3b-I ratio, were detected by Western blotting from whole retinal extracts.

161 and traffic by confocal microscopy, western blotting, gel filtration, and immunoprecipitation studie

162 ssay (VOPBA) in combination with far-Western blotting, gradient-purified EAV particles were shown to

163 Blotting has been the standard technique for preparing a

164 In the present study, Western blotting identified a band corresponding to C3 in freshl

165 in the gastrocnemius muscle (GM) by western blotting; IKKalpha/beta, JNK, ERK 1/2, NF-kappaBp65, and

166 lyp tissue were analyzed by means of Western blotting, immunoassays, or histology.

167 uced autophagosomes was confirmed by Western blotting, immunofluorescence microscopy, and immunoelect

168 ere validated by immunoprecipitation/Western blotting, immunofluorescence, and proximity ligation ass

169 Using western blotting, immunofluorescence, ELISA and qRT-PCR, we inve

170 commercially available antibodies by western blotting, immunofluorescence, immunocytochemistry and fl

171 established and then validated using western blotting, immunohistochemistry, and comparable TCGA RPPA

172 As judged by Western blotting, immunohistochemistry, and ELISA, rh Bri2 BRICH

173 scription polymerase chain reaction, Western blotting, immunohistochemistry, and fluorometric assays.

174 Using molecular cloning, RT-PCR, Western blotting, immunolocalization and in vitro functional ass

175 xposed mice, and GCS was detected by Western blotting in chronic obstructive pulmonary disease lungs

176 eaved) C3 fragments were detected by Western blotting in extracts of IL-1alpha-stimulated cartilage.

177 nerated and verified using q-PCR and western blotting in human keratinocytes (HaCaT) and endothelial

178 ochemistry on kidney sections and by Western blotting in plasma samples from rats subjected to renal

179 ex with EZH2, which was validated by western blotting in vitro.

180 ent mammalian cells with single-cell western blotting (in situ scWB) uses microfluidic design to elim

181 strate their superior performance in Western blotting, in both peroxidase- and fluorophore-linked for

182 chain reaction, flow cytometry, and Western blotting-in several nonprostatic cell lines and xenograf

183 or partners by mass spectrometry and western blotting, including DDX6, UNR, unrip, PAT1B, LSM14A and

184 Western blotting indicated that NMII-induced B1a cell death was

185 ted cells by electron microscopy and Western blotting indicated that PI3K-C2A was not required for th

186 e ancient p62 primary sequence, with Western blotting indicating p62 abnormalities, including in dent

187 Western blotting is a commonly used protein assay that combines

188 surface glycosylation, and for tissue paper blotting is likely due to cell permeabilization.

189 Western blotting is most often used for protein identification o

190 This improved northern blotting is particularly useful to analyze products of R

191 Using Western blotting, kinetic assays, and microfluidic analyses, we

192 A (quantitative RT-PCR) and protein (western blotting) levels.

193 lysosome compartment evaluation, and western blotting light chain 3 (microtubule-associated protein 1

194 The study used biochemical (e.g., Western blotting, mass spectrometry) and biophysical assays (e.g

195 tion by quantitative Western and far-Western blotting, mass spectrometry, and computational modeling.

196 nd reporting in immunoassays such as Western blotting may promote improved reproducibility across the

197 However, traditional Western blotting methods provide unreliable quantification and h

198 ntitative real time PCR (n = 21) and western blotting (n = 9).

199 med on CSF, and the second option is Western blotting of a brain biopsy specimen used to detect prote

200 tectable by both inverse RT-PCR and northern blotting of HPV16-transformed cells.

201 Western blotting of MKK2 protein and its T31 phosphorylated pept

202 ule (MT)-associated EB1 tracking and Western blotting of MT posttranslational modifications indicated

203 To address this question, we used Western blotting of postmortem tissue from human V1 (12 female,

204 t complement RNA-seq, discover that northern blotting of small RNAs is biased against short sequences

205 ependent MGMT silencing confirmed by Western blotting of tumor tissue ex vivo.

206 ch cell in the microwell and perform western blotting on each resultant lysate.

207 Western-blotting on mouse cervix confirmed large scale histone d

208 or spleen of these animals by either Western blotting or after amplification by serial PMCAb.

209 for MPC2 in click chemistry-enabled western blotting or global mass spectrometry-based proteomic exp

210 chemical processes were evaluated by Western blotting or immunofluorescence.

211 tative label-free mass spectrometry, Western blotting, or confocal imaging.

212 in expression as measured by RT-PCR, Western blotting, or Luminex technology.

213 As shown by immunohistochemistry and Western blotting, PPARalpha was downregulated in the corneas of

214 reactivity with well-developed HIV-1 Western blotting profiles by 24 weeks after infection.

215 were here integrated with optimized western blotting protocols in solving the complex IEF pattern of

216 Confocal microscopy and western blotting provided evidence of the formation of a covalen

217 Echocardiography, Western blotting, qPCR, immunohistochemistry, immunofluorescence

218 escence and confocal microscopy, and western blotting quantification of the protein phosphorylation w

219 field and rotarod tests, followed by Western blotting, quantitative PCR, and immunohistochemistry to

220 ort of human brain tissue samples by Western blotting, quantitative PCR, and immunohistochemistry.

221 a) pathways, which were validated by Western blotting, quantitative polymerase chain reaction of sele

222 ed in detection of miRNAs including northern blotting, quantitative real time PCR (qRT-PCR) and micro

223 l outcomes were assessed by means of Western blotting, real-time PCR, differentiation, and proliferat

224 le analysis, morphologic assessment, Western blotting, receptor binding, gene expression, small inter

225 Tissue paper blotting removed GalNAz-labeled surface cells, causing D

226 in levels were measured by ELISA and Western blotting, respectively, in blood samples from 101 patien

227 etected with quantitative RT-PCR and Western blotting, respectively, in both platelets and Meg-01 cel

228 were assessed by flow cytometry and Western blotting, respectively.

229 was measured using real-time PCR and western blotting, respectively.

230 Western blotting results also revealed decreased protein express

231 Immunofluorescence microscopy and Western blotting results showed that RSV infection of human airw

232 Southern blotting revealed btl genes in 14 diverse Mycetohabitans

233 Immunofluorescence microscopy and western blotting revealed marked disorganization and reduced COL

234 IHC and western blotting revealed reduction in epidermal hyperplasia (Ki

235 Western blotting revealed that protein expression of TLR4 was ma

236 resonance energy transfer (FRET) and western blotting revealed the activation of Src in ECs was signi

237 Western blotting revealed the presence of Pph (Ptb33) and Tph (T

238 with real-time quantitative PCR and Western blotting revealed up-regulation of C3 and CFB expression

239 s, supplemented by real-time PCR and Western blotting, revealed that the expression of critical regul

240 tional western blotting, single-cell western blotting (scWB) is particularly useful for protein targe

241 Western blotting showed expression of sialin, a known nitrate tr

242 Northern blotting showed that transcription of 2.4/2.1-kb mRNA co

243 Like conventional western blotting, single-cell western blotting (scWB) is particu

244 uencing, quantitative real-time PCR, Western blotting, small interfering RNA interference, and kinase

245 Quantitative RT-PCR and Northern blotting studies showed reduced or loss of expression of

246 and the severe loss of calpain 3 on western blotting, suggest a dominant negative effect with a loss

247 Western blotting suggested beta-conglutins were the main protein

248 ream regulator analysis coupled with Western blotting suggests that abnormal basal activation of the

249 Northern blotting suggests that these anti-CRISPRs manipulate sin

250 Using mass spectrometry and Western blotting techniques, we have identified choline transpor

251 On Western blotting, the mean ratios of pNFKB:NFKB for control and

252 Contrary to common Western blotting, the separation occurs in nondenaturing conditi

253 iChemR23 was examined by qRT-PCR and Western blotting.The roles of the MAPK and PI3K-Akt signaling pa

254 .3 fg or ~10(4) molecules, whereas the total blotting time is about 5 min.

255 from an "enclosed" microchannel for PAGE and blotting to an "open" PA gel lane for immunoprobing and

256 We used RNA sequencing and Western blotting to analyze the signaling pathways regulated by

257 s, fast scan cyclic voltammetry, and western blotting to examine whether sleep/wake state and/or ligh

258 (Ser235/Ser236) was performed using Western blotting to infer the degree of activation of AMPK signa

259 tative polymerase chain reaction and Western blotting to investigate changes in ion channel expressio

260 we have used immunoprecipitation and Western blotting to show that in response to LPS, LPCAT2, but no

261 Toure et al, 2004 used Northern blotting to show that the Y-linked genes Ssty1 and Ssty2

262 otein acetylation levels in cells by western blotting (tubulin vs histone acetylation), and by assess

263 the protein acetylation in cells by Western blotting (tubulin vs histone acetylation).

264 igomers, whereas immunoprecipitation-Western blotting using high-detergent extracts revealed a variet

265 cipitation and simultaneous assay by Western blotting using multiplex fluorescence imaging with speci

266 ability by segregation analysis and Southern blotting using postmortem brain samples from two affecte

267 pectrometry, and further verified by Western blotting using specific Abs.

268 These were further validated by Western blotting using specific Abs.

269 duced as shown by flow cytometry and Western blotting, using strains expressing different glutamine l

270 Dot blotting was also used to analyze expression changes in

271 Additionally, quantitative Western blotting was carried out for pNFKB and NFKB in specimens

272 Western blotting was carried out to investigate expression level

273 Western blotting was performed to detect endogenous and overexpr

274 ide concentrations were measured and western blotting was performed to investigate pathways affected

275 Western blotting was performed to measure levels of total-Tau, p

276 At the same time Western blotting was performed with the patient's serum of previ

277 transfection with miR-146b mimic and western blotting was used to analyse SOX5.

278 e determined by neutral comet assay; western blotting was used to evaluate protein changes; changes i

279 Western blotting was used to investigate the bladder expression

280 J protein expression was assessed by Western blotting (WB) and immunocytochemistry (ICC).

281 The utility of Western blotting (WB) for the serodiagnosis of canine bartonello

282 ssociated prion protein (PrP(Sc)) by Western blotting (WB), antigen capture enzyme immunoassay (EIA),

283 n immunochemical techniques, such as western blotting (WB).

284 (TDC) has been successfully used in Western blotting (WB).

285 Based on lipid analysis and Western blotting, we show that the bacteria-like entities consis

286 ong immunoreactivity around 70kDa on Western blotting were also anti-Drebrin-positive.

287 assay, flow cytometry analysis, and Western blotting were applied to evaluate the activation of rela

288 ing mass spectrometry, dot blots and Western blotting were developed to determine the level of Ara h

289 ceptor (GHRH-R) measured by qPCR and Western blotting were significantly down-regulated in retinas of

290 qPCR and western blotting were used to assess ion channel expression.

291 Microscopy and Western blotting were used to determine activation of signal tra

292 l-time polymerase chain reaction and Western blotting were used to evaluate Renal Klotho expression.

293 Western Blotting with anti- WFhb1-1 antibody revealed a signific

294 r binding partners were determined by ligand blotting with CLC/Gal-10, followed by coimmunoprecipitat

295 We performed Western blotting with enokitake extracts and the patient's serum

296 nse gene 88), or after superficial injury by blotting with tissue paper.

297 (n = 20)) by automated quantitative western blotting, with excellent agreement with our proteomics f

298 p using a purification procedure and Western blotting, with sera from the patient and several control

299 Western blotting would be a simple end point detection method fo

300 Cell viability, Western Blotting, Zymogram, and Real-time PCR analyses were perf