ライフサイエンスコーパス: cDNA

1 cDNA microarray analysis of TNBC cells stably integrated

2 cDNA microarray data were collected both from patients e

3 cDNA studies demonstrated that the c.1014+1G>A variant c

4 cDNA with 5 A's may yield novel Gag product(s), while cD

5 cDNA-TCR beta-chain libraries were sequenced from 2 mill

6 cDNAs were constructed by site-directed mutagenesis that

7 -hsa-anti-miR-34a or -449a or through PACS-1 cDNA transfection led to the reversal of DNA damage resp

8 Following mRNA purification, 10 cDNA libraries were assessed by RNA-seq.

9 ect as low as 200 ymol, corresponding to 120 cDNA molecules of L-gene Ebola virus with a limit of det

10 , WPMIAS supports 64 plant species with ~200 cDNA libraries and 274 pre-loaded plant degradome datase

11 Similarly, transfection of AP-2alpha cDNA decreased TACE promoter luciferase activity, TACE e

12 virus that delivers human interferon alfa-2b cDNA into the bladder epithelium, and a novel intravesic

13 uence through recombination of the 5' and 3' cDNAs, leading to the durable restoration of otoferlin e

14 Ebola virus with a limit of detection of 33 cDNA molecules.

15 g enzymes prior to rapid amplification of 5' cDNA ends (5' RACE) for HIV-1 RNA and quantitative rever

16 We amplified a cDNA from the salivary glands of the tropical bont tick

17 Creating a cDNA library for deep mRNA sequencing (mRNAseq) is gener

18 For a cDNA of ~450 bp, about half of the expressed proteins we

19 at a single intraperitoneal inoculation of a cDNA clone encoding an attenuated rZIKV was safe, highly

20 Sequencing of a cDNA encoding ArPPLNP2 revealed that it comprises eleven

21 Here we report the identification of a cDNA for a DGAT1-type enzyme, designated CpuDGAT1, from

22 cy (SCID-X1) using targeted integration of a cDNA into the endogenous start codon to functionally cor

23 Here we report that a cDNA encoding a full-length, approximately 45-kDa K15P r

24 Transfection of CD4- 293T cells with a cDNA expression library developed from a podocyte cell l

25 eplaced with human FGFR3(G380R) (FGFR3(ACH)) cDNA, the most common mutation in human ACH.

26 both structural (gDNA content) and activity (cDNA expression) levels.

27 cells that were modified with the human ADA cDNA (MND-ADA) gamma-retroviral vector after conditionin

28 f iCLIP and related methods that can amplify cDNAs that truncate at crosslink sites and we show that

29 We used Illumina RNA-Seq to analyse cDNA libraries for differential expression of genes from

30 DNA and cDNA 16S rRNA gene profiling demonstrated that the micro

31 next-generation (nextGen) paired-end DNA and cDNA sequencing as input, call on several well establish

32 Sample material (gDNA and cDNA) of a total of 49 patient-individual CRC cell lines

33 mediated by the Ty3 intasome complex (IN and cDNA) is subject to inputs from a combination of host fa

34 via recombination between such plasmids and cDNA copies of capsid genes of eukaryotic positive-sense

35 encing with methylation-specific primers and cDNA analysis in patient neurons indicated selective exp

36 c 346 kb inversion in multiple probands, and cDNA sequencing and a splicing assay established that tw

37 argets independent of other Ty3 proteins and cDNA.

38 ZO-1 small interfering RNA and cDNA transfection experiments emphasized regulation of C

39 RNA-mediated genomic modifications: RNA- and cDNA-templated DSB repair (R-TDR and c-TDR) using an RNA

40 In the bulk method, sorting and cDNA library steps are replaced with a reverse-transcrip

41 Tissue and cDNA microarrays of PCa were analyzed for NO66 mRNA and

42 sing a CAV1 scaffolding domain construct and cDNAs encoding wild-type CAV1, and CAV1 phosphorylation

43 noma cells (SK-MEL-28) transfected with APCN cDNA acquired the ability of invasive growth in semisoli

44 Overexpression of APCN (cDNA) in various cell lines induced sprouting of slender

45 By screening the Arabidopsis cDNA library using yeast-2-hybrid interaction, we identi

46 extent of multimer expression decreasing as cDNA length increased.

47 nts based on expression technologies such as cDNA microarrays, RNA-Seq, and cell imaging-based assays

48 uitable for downstream applications, such as cDNA synthesis, gene amplification, and RT-qPCR.

49 (ATF4) and overexpression of exogenous ATF4 cDNA indicated that ATF4 up-regulates LAMP3 mRNA levels.

50 identify samples and cells, and the barcoded cDNAs are pooled into a library pool before high through

51 Through cell survival-based cDNA expression screens in neural progenitor cells, we i

52 Wild-type and mutant bCYP3A cDNAs were cloned and expressed in V79 cells.

53 ta vector encoding the human interferon-beta cDNA (hIFNbeta) was used to transduce human melanoma cel

54 We then overexpressed a blw cDNA construct driven by either the AG or GT haplotype p

55 the AG haplotype also results in greater blw cDNA expression and a significant decrease in lifespan r

56 e explored the genetic diversity of 125 Bm86 cDNA gene sequences from R. microplus from 10 endemic ar

57 By cDNA library screening, we identified an immune cell-spe

58 ysis of finger millet (Eleusine coracana) by cDNA subtraction identified drought responsive genes tha

59 seq (DNA-RNA immunoprecipitation followed by cDNA conversion coupled to high-throughput sequencing),

60 water-in-oil emulsion droplets, followed by cDNA sequencing.

61 fied, and most of the genes are validated by cDNA and RNA-seq data.

62 is of PREX1 mRNA expression in breast cancer cDNA arrays and a METABRIC cohort revealed that higher P

63 ybrid screenings of placenta and lung cancer cDNA libraries, which demonstrated that the long IDR lin

64 The CAR cDNA is genetically integrated in the T cell genome.

65 en using dysbindin as bait against a cardiac cDNA library to identify the cardiac dysbindin interacto

66 TALEN technology and a 7bp deletion in CERKL cDNA that caused the premature termination of CERKL.

67 nsfection with increasing amounts of WT-CFTR cDNA progressively increased SLC26A9 levels in F508del-C

68 Paired heavy and light chain cDNAs from dominant plasmablast clones were expressed as

69 n, we cloned and biochemically characterized cDNA encoding CBS from T. gondii (TgCBS), which represen

70 the endogenous fusion protein or a chimeric cDNA leads to the formation of indolent liver tumors in

71 Our group originally found and cloned cDNA for a 98-kDa type 1 transmembrane glycoprotein of u

72 eotide primers for qRT-PCR assays and cloned cDNA plasmids corresponding to the Defa paralogs.

73 sgene, TgAC1, consisting of Clrn1-UTR (Clrn1 cDNA including its 5' and 3' UTR) under the control of r

74 lin-like peptide cloned from the Aplysia CNS cDNA replicated both the enhancement of synaptic transmi

75 m gene addition studies using protein-coding cDNAs to the modulation of gene expression using small R

76 g PacBio Iso-Seq, a total of 31,133 complete cDNA sequences were obtained in the fruiting body.

77 now find that this is caused by constrained cDNA-ends, which can result from the sequence and struct

78 d a functional genomic screen using a cotton cDNA library in a virus-induced gene silencing (VIGS) ve

79 Furthermore, insertion of a Cxcl7 cDNA in the lentiviral vector that carries a Prkcd shRNA

80 actin remodeling processes: wild-type DAAM2 cDNA, but not cDNA representing missense variants found

81 support the concept of retroelement-derived cDNA as key triggers of systemic autoimmunity in Trex1-d

82 t relies on the acquisition of viral derived cDNA (vDNA) and how this pathway discriminates between s

83 and a small subunit for which two different cDNAs (LiGPPS.SSU1 and LiGPPS.SSU2) were detected.

84 arisons, we also perform relevant ONT direct cDNA- and Illumina-sequencing.

85 hput method, we developed a T7 phage display cDNA library derived from mRNA isolated from bronchoalve

86 of genomic DNA (gDNA) and complementary DNA (cDNA) and metatranscriptomics to investigate how the acq

87 is undertaken by deriving complementary DNA (cDNA) from puromycin-treated patient lymphoblasts, hybri

88 by screening a tooth germ complementary DNA (cDNA) library using a yeast 2-hybrid system.

89 rand synthesis to recover complementary DNA (cDNA) molecules that were successfully reverse transcrib

90 reaction (PCR)-amplified complementary DNA (cDNA) on the GMR for the reference gene GAPDH.

91 ngle-molecule full-length complementary DNA (cDNA) sequencing can aid genome annotation by revealing

92 y noted, the positions of complementary DNA (cDNA)-starts depend on cDNA length in several iCLIP expe

93 beta polypeptide (ATP7B) complementary DNA (cDNA; AAV8-ATP7B) is able to provide long-term copper me

94 Seven complimentary DNA (cDNA) fragments spanning the SARS-CoV-2 genome were asse

95 ions were found in CD247 complementary DNAs (cDNAs) cloned from the patient as well as in cDNA and ge

96 To rescue Kv4 complex downregulation, cDNA constructs encoding Kv4.3, KChIP1, and DPP10 were t

97 d loss-of-function screens using a human DUB cDNA library of 65 genes and an siRNA library of 98 gene

98 und by a deletion signature generated during cDNA synthesis after bisulfite treatment for which the c

99 lecule to eliminate errors introduced during cDNA synthesis, PCR, and sequencing.

100 hodology to (1) select only human RNA during cDNA synthesis by aiming at the poly(A)+-tail and (2) in

101 reorientation, causing base-skipping during cDNA synthesis.

102 a unique molecular identifier (UMI) to each cDNA molecule to improve the accuracy of repertoire-freq

103 he development of a relatively fast and easy cDNA-based system for the semi high-throughput functiona

104 We cloned the full-length EB1 cDNA for its overexpression in the testis, which was fou

105 Ebola cDNA was amplified by rolling circle amplification (RCA)

106 PSE, which are orthologs of M-IPSE, from egg cDNA of S. haematobium Using PCR and immunodetection, we

107 unya fever (CHIKF) pandemic, we used an EILV cDNA clone to design a chimeric virus containing the chi

108 yed an in silico screen for cardiac-enriched cDNAs.

109 rescued by overexpression of wild-type EXTL3 cDNA.

110 iopsies were taken and total RNA extraction, cDNA synthesis and PCR was performed using 10 candidate

111 SlCAT2 was cloned from tomato flower cDNA, over-produced in Escherichia coli and purified by

112 tected in less than 2 h without the need for cDNA synthesis or any other enzymatic reactions and with

113 role in site-specific cleavage of TR RNA for cDNA priming.

114 reening of a human cDNA library, looking for cDNAs able to rescue yeast growth.

115 ting expression plasmids with Foxm1 or Foxf1 cDNAs were injected intravenously.

116 adaptor ligation, followed by fragmentation, cDNA generation, PCR amplification, and deep sequencing.

117 g efficient recovery of infectious HAZV from cDNA.

118 rt a system that is able to rescue HAZV from cDNAs, thus permitting reverse genetic interrogation of

119 notypes that cannot be rescued by functional cDNA expression, suggesting that RPS25 loss elicits a ce

120 by the transduction of mouse BM with fusion cDNAs derived from human leukemias.

121 addition, supplementation of the target gene cDNA into the otocysts of homozygous Slc24a4 knockout mi

122 me PCR assays for human erythropoietin gene, cDNA or transcript, we found that inclusion of yeast RNA

123 utated to alanine using a type A full-genome cDNA clone, and the virus progeny was analyzed for defec

124 RNA transcribed from the full-genome cDNA was highly infectious after electroporation into ce

125 V-2 genome were assembled into a full-genome cDNA.

126 gene-expression profiling using whole genome cDNA-mediated annealing, selection, extension, and ligat

127 Using whole-genome cDNA microarray technology (Illumina), we examined GE in

128 related recombination mechanisms and genomic cDNA-like sequences that implicate evolutionary origins

129 proteins from the P. papatasi salivary gland cDNA library.

130 omparison, nRT-PCR quantified the target HAV cDNA with a limit of detection of 6.4fg/microL.

131 intracellular dNTP pools, and facilitate HIV cDNA synthesis.

132 e sheep expressing a human CAG-expansion HTT cDNA transgene.

133 to perform a functional screening of a human cDNA library, looking for cDNAs able to rescue yeast gro

134 By screening human cDNA library, we uncover that the Golgi resident protein

135 on, but the introduction of feline and human cDNAs rendered them permissive.

136 high-throughput RNA sequencing by impairing cDNA synthesis.

137 cDNAs) cloned from the patient as well as in cDNA and genomic DNA from other individuals, suggesting

138 iation and mismatches of alleles detected in cDNA and gDNA suggesting that some loci have undergone p

139 represent full transcripts due to incomplete cDNA synthesis and sequencing length limits.

140 ice genotype (Hasawi), a salt-stress-induced cDNA expression library was constructed and subsequently

141 , we report the development of an infectious cDNA clone for the SVA/HLJ/CHA/2016 strain.

142 aring the 197-aa deletion from an infectious cDNA clone of the highly virulent PEDV PC22A strain (inf

143 WV, we designed a series of novel infectious cDNA clones corresponding to coexisting DWV genotypes, t

144 f the 2'-O-MTase using a virulent infectious cDNA clone, icPC22A, as the backbone.

145 SpISO-seq requires less than 1 ng of input cDNA, limiting or removing the need for prior amplificat

146 ned and characterized Lavandula x intermedia cDNAs encoding geranyl diphosphate synthase (LiGPPS), ge

147 d quality, and then reverse transcribed into cDNA before being subjected to real-time qRT-PCR analysi

148 (sequencing of RNA reverse transcribed into cDNA) and found that neurogenesis and haematopoiesis dom

149 ides is efficiently reverse transcribed into cDNA, and we develop an assay to measure the combined fi

150 ntrol groups) for reverse transcription into cDNA, preamplification and then real time quantitative p

151 n isopentenyl pyrophosphate isomerase (IPPI) cDNA, AaIPPI1, from Artemisia annua (Aa).

152 Overexpression of the reference IQSEC1 cDNA in wild-type flies is lethal, but overexpression of

153 but overexpression of the two variant IQSEC1 cDNAs did not affect viability.

154 ll-centric and gene-centric modes to isolate cDNA fragments from scRNA-seq libraries.

155 tive phosphoproteomics with mammalian kinome cDNA library screen.

156 FN-I response is triggered by cytoplasmic L1 cDNA, and is antagonized by inhibitors of the L1 reverse

157 k (-/-) mice in vivo with both Pjvk and Lc3b cDNAs completely restored sound-induced pexophagy, fully

158 We first constructed a stable full-length cDNA clone of ZIKV in a novel linear vector from which i

159 We report the full-length cDNA cloning, molecular characterization and functional

160 rms because it fails to sequence full-length cDNA copies of RNA molecules.

161 The full-length cDNA encodes a type-I IPPI containing a plastid transit

162 We report nanopore sequencing of full-length cDNA from CLL samples with and without SF3B1 mutation, a

163 (PMA) and transduction with the full-length cDNA of GLS2.

164 Here, we report the full-length cDNA sequence of croaker elovl4, which contained 1794 bp

165 re, we generated over 10,000,000 full-length cDNA sequences at a median accuracy of 97.9% using our n

166 (1) show that deep and accurate full-length cDNA sequencing can be used to provide isoform-level tra

167 fication of cDNA ends (RACE) and full-length cDNA sequencing, revealed four independent promoters, pr

168 equences of natural Rhi o 2, the full-length cDNA was cloned, and expressed as recombinant (r) allerg

169 e isolated and characterized the full-length cDNA, gDNA and a putative promoter of a RIOK-2 protein k

170 Here, two ELO full-length cDNAs (TmELO1, TmELO2) from the yellow mealworm (Tenebri

171 and Pacific Biosciences (PacBio) full-length cDNAs to identify 104,091 high-confidence protein-coding

172 ntiviral complementation with wild-type MDH2 cDNA restored MDH2 levels and mitochondrial MDH activity

173 ecome the gold standard technique to measure cDNA and gDNA levels but the resulting data can be highl

174 MEF2C cDNA resistant to CRIPSR cutting rescued MEF2C knockout

175 hybridization-based targeted RNA-Seq method, cDNA-smMIPs are a cost-effective high-throughput tool fo

176 lysis of human RCC tumor tissue microarrays, cDNA arrays and tumor biopsy samples demonstrated V2R ex

177 Using over 7 million cDNA sequences from both pyrosequencing and Sanger seque

178 ion pipelines by integrating single-molecule cDNA-sequencing data generated from either the Pacific B

179 enerated recombinant proteins from the mouse cDNAs encoding the Hsp90alpha-Delta and wild-type Hsp90a

180 tering nanobodies using an animal-free, mRNA/cDNA display technology.

181 ns, most of which were supported by multiple cDNA evidence (72%) while only 20% of them have coding c

182 nes RNA barcoding and generation of multiple cDNA copies per RNA molecule to eliminate errors introdu

183 leotides (+CCC) to the 3'-end of the nascent cDNA, which can then anneal to the matching rGrGrG 3'-en

184 ntiviral transduction with wild-type NDUFAF8-cDNA ameliorated both the assembly defect and the bioche

185 ing processes: wild-type DAAM2 cDNA, but not cDNA representing missense variants found in individuals

186 ) Polymerase-Mediated Rapid Amplification of cDNA Ends (PPM-RACE).

187 licing patterns using rapid amplification of cDNA ends (RACE) and full-length cDNA sequencing, reveal

188 A were verified by 3' rapid amplification of cDNA ends (RACE) and Northern blot analyses in several E

189 HBV DP-rcDNA by 5'/3' rapid amplification of cDNA ends (RACE), 5' radiolabeling, and exonuclease dige

190 Using 3' rapid amplification of cDNA ends (RACE), we mapped the 3' end of the N and NSs

191 A rapid amplification of cDNA ends assay, DNA sequencing, and Northern blot revea

192 ration sequencing and rapid amplification of cDNA ends obtained the complete genome.

193 re sequencing on RNA (rapid amplification of cDNA ends-based repertoire sequencing [RACE-RepSeq]), we

194 ffected plants employing massive analysis of cDNA ends (MACE) and RT-qPCR.

195 sequencing based on the massive analysis of cDNA ends approach, which was validated using high-throu

196 Analysis of cDNA from family 2 revealed retention of the final intro

197 Analysis of cDNA from lymphoblastoid cells demonstrated partial spli

198 Analysis of cDNA libraries specific for transcripts bearing a 5'-tri

199 g of single cells into plates, generation of cDNA libraries, a TCR-specific amplification step, a sec

200 Overexpression of cDNA representing SGPL1 mutations resulted in subcellula

201 y annotated using morphological profiling of cDNA constructs, via a microscopy-based Cell Painting as

202 at enable highly multiplexed resequencing of cDNA target regions of approximately 100 nucleotides and

203 activation domain of YAP1, and sequencing of cDNA from the patient shows it does not result in nonsen

204 Sequencing of cDNA generated from community rRNA revealed that diverse

205 eloped kinetic cross-linking and analysis of cDNAs (chiCRAC), an ultraviolet cross-linking method tha

206 reported the functional characterization of cDNAs encoding short-chain isoprenyl diphosphate synthas

207 a complementary nucleotide to the 3' end of cDNAs synthesized from natural templates.

208 us-based system allows the overexpression of cDNAs of up to 2,100 nucleotides (encoding a protein of

209 contrast, we show that a broad size range of cDNAs in iCLIP allows the cDNA-starts to efficiently del

210 matics, we performed long-read sequencing of cDNAs from Arabidopsis (Arabidopsis thaliana) lines defi

211 s required for the efficient catalysis of (-)cDNA synthesis during viral infection before capsid unco

212 of complementary DNA (cDNA)-starts depend on cDNA length in several iCLIP experiments and we now find

213 f these protein isoforms, relying instead on cDNA-based transgene expression.

214 Null-allele test on cDNA from patients' fibroblasts of both families carryin

215 we show that computational analyses based on cDNAs-starts are appropriate for such methods.

216 n of AAV particles harboring codon-optimized cDNAs encoding HLA-G1 and HLA-G5 isoforms one week prior

217 RISPR/Cas9-mediated loss of function and ORF/cDNA driven rescue screens, and cell-based models of spo

218 hylogenetic analysis revealed 20 OsPLDalpha1 cDNA variants which further translated into 12 protein v

219 entified gencDNAs have similarities to other cDNA-like sequences existing throughout phylogeny, inclu

220 ' and the other the 3' portions of otoferlin cDNA, which exceed the packaging capacity of the AAV whe

221 -/-) mutant mice reconstituted the otoferlin cDNA coding sequence through recombination of the 5' and

222 can be rescued by a single short human OXR1 cDNA that only contains the TLDc domain.

223 We performed cDNA and direct RNA sequencing analyses and revealed an

224 ls of peroxisome proliferation, whereas Pjvk cDNA alone yielded only a partial correction of the defe

225 lant species, two class III patatin-like PLA cDNAs (pPLAIIIbeta or pPLAIIIdelta) from castor or Physa

226 erent graft combinations was used to prepare cDNA libraries for small RNA sequencing and to analyze m

227 single-molecule molecular inversion probes (cDNA-smMIPs) that enable highly multiplexed resequencing

228 Through probing cDNA extension mediated by Bst DNA polymerase at and nea

229 s vector delivering murine or human proSFTPB cDNA into SP-B deficient mice restores surfactant homeos

230 body-producing B cell clones at the protein, cDNA and gDNA levels, we characterized additional LAIR1

231 temperature and reaction time using purified cDNA and viral RNA as template.

232 We deduced the full-length of the RBM28 cDNA sequence and profiled its expression patterns detec

233 tion pipeline utilizing short- and long-read cDNA sequencing, protein evidence, and ab initio predict

234 RACRs, particularly those carrying a recoded cDNA-restoring endogenous gene activity, can drive relia

235 y moderate expression of the CDK19 reference cDNA but not by expression of the two variants.

236 essed by expression of human CDK19 reference cDNA.

237 y moderate expression of the human reference cDNA.

238 abbits by coimmunization with the respective cDNAs.

239 Feline and human RFC cDNAs conferred susceptibility to TG35-2-pseudotyped vir

240 our previously described infectious ZIKV-RGN cDNA clone, we identified a natural polymorphism in the

241 d a Y1H system to screen a salt induced rice cDNA expression library from Hasawi.

242 In vivo assays show that this hybrid RNA-cDNA molecule is required for mutagenic transposition, r

243 ughput methods to study the effects of RNAi, cDNA, and chemical libraries, have evolved to encompass

244 ter intraperitoneal inoculation of the rZIKV cDNA in the absence of transfection reagent.

245 activity in cells transfected with SERPINB7 cDNA carrying the mutation and promoted full-length SERP

246 .myc to drive expression of the human SH3TC2 cDNA under the control of the Mpz promoter specifically

247 of 468 single cells and 1297 matched single cDNA samples, performing SMARTer and Smart-seq2 protocol

248 d dimers of the Broccoli aptamer into a SINV cDNA clone using sites in nsP3 (genomic RNA), the 3'UTR

249 However, inclusion of a DENV-specific cDNA primer did increase the viral genome coverage immed

250 of p22phox as bait to screen a human spleen cDNA library, we identified the protein interacting with

251 idic device to synthesize and deliver stable cDNA for downstream gene expression analysis, thereby al

252 nstrated that nanoparticle delivery of STAT3 cDNA into the neonatal circulation restored endothelial

253 at hybridizes to a complementary DNA strand (cDNA) to form a double-stranded DNA (dsDNA).

254 against the constriction region, subsequent cDNA strand insertion inside the nanopore's beta-barrel

255 erent ratios of rat alpha4 and beta2 subunit cDNA were transfected into human embryonic kidney 293 ce

256 enotypes were rescued by expression of SURF4 cDNA.

257 ng technology combined with Primer ID-tagged cDNA primers to efficiently quantify HIV-1 splicing at a

258 n-chip hybridization of complementary target cDNA) is decreased with increasing the ionic strength of

259 ibed Valpha3.2 Vbeta14 T cell receptor (TCR) cDNAs, the dominant clonotype generated in splenocytes a

260 The cDNA library was transfected into C6/36 (Aedes) and Vero

261 The cDNA sequences revealed that dsRNA1 dsRNA is 1,683 bp in

262 road size range of cDNAs in iCLIP allows the cDNA-starts to efficiently delineate the complete RNA-bi

263 Using TriSilix, we also detect the cDNA from SARS-CoV-2 (1 pg) with high specificity agains

264 A 300 bp specific fragment from the cDNA fragment was chosen to insert into vector pFGC1008

265 reated patient lymphoblasts, hybridizing the cDNA to the BROCA panel of tumor suppressor genes, and t

266 s gene expression and it was proved that the cDNA fragment was relevant to the cellulose biosynthesis

267 tomato phytaspase genes were identified, the cDNAs were cloned and the recombinant enzymes were obtai

268 These cDNAs were expressed in transgenic plants of a PORB-defi

269 dine-to-uridine-driven hypermutation of this cDNA.

270 nsic to the most widely used high-throughput cDNA sequencing technologies.

271 ded RNA from 312 maize cobs was converted to cDNA, and sequences were determined using an Illumina Hi

272 ethyl group on these modified bases prior to cDNA synthesis using enzymes.

273 i-GAL4-driven expression of human UAS-TOMM70 cDNA.

274 uplexes via a linker; reverse transcription; cDNA library amplification; and finally high-throughput

275 Here, we isolated two cDNAs from mature or imbibed cucumber seeds with high se

276 e same line reconstituted with VEC wild-type cDNA.

277 plification (Ct values <= 35 for unamplified cDNA) had to be replaced by (3) proofing linear pre-ampl

278 A-seq library preparation to remove unwanted cDNA from 16s ribosomal rRNA without increasing the numb

279 In this study, we used cDNA microarray data to determine APLNR expression level

280 scanning (DMS) strategy(2-5) whereby a viral cDNA library was constructed containing all codon substi

281 nvestigate their role using a cochlear viral cDNA transfer approach in vivo, where IHCs of mouse lack

282 d cleave A3G-edited uridine-containing viral cDNA.

283 iting HIV-1 replication by deaminating viral cDNA cytosines and interfering with reverse transcriptio

284 nfectivity factor (Vif) by deaminating viral cDNA cytosines to uracils.

285 plementary ssDNA and 6.94fg/microL for viral cDNA.

286 ugh the combined effects of inhibiting viral cDNA production and cytidine-to-uridine-driven hypermuta

287 onds to defects in the accumulation of viral cDNA in the nucleus.

288 After next-generation RNA sequencing, viral cDNA clones mimicking the enterovirus RNA sequences foun

289 retrovirus integrase (IN) inserts the viral cDNA into the host DNA genome.

290 h a lentiviral vector encoding for human WAS cDNA.

291 locus to drive the UAS-human reference WDR37 cDNA.

292 ce in situ hybridization with a set of wheat cDNAs allowed the macrostructure and cross-genome homoeo

293 ICA was able to detect <= 141 fg of RNA when cDNA used as a template.

294 5 A's may yield novel Gag product(s), while cDNA with an extra base, 7 A's, may only be a minor cont

295 7 nAChRs with NACHO, we utilized genome-wide cDNA library screening and discovered that several anti-

296 of both sexes) were virally transduced with cDNAs of various mini-otoferlins.

297 nels, we employed HEK cells transfected with cDNAs encoding three requisite receptor subtypes: alpha7

298 irect delivery in mice of an infectious ZIKV cDNA clone allows the rescue of recombinant (r)ZIKV in v

299 , the mutants carrying the transgenic ZmNLP5 cDNA fragment significantly increased the nitrate conten

300 ls, partially restored following TLN1 or ZYX cDNA overexpression.