ライフサイエンスコーパス: carbamoyl phosphate

1 t phosphodonors such as acetyl phosphate and carbamoyl phosphate.

2 l reactions but not the overall synthesis of carbamoyl phosphate.

3 biosynthesis by catalyzing the production of carbamoyl phosphate.

4 orylation of carbamate to the final product, carbamoyl phosphate.

5 droxybenzoic acid (AHBA), D-glucosamine, and carbamoyl phosphate.

6 ichaelis constants for either bicarbonate or carbamoyl phosphate.

7 erized with or without prior incubation with carbamoyl phosphate.

8 nucleotides used in the overall synthesis of carbamoyl phosphate.

9 rst ATP utilized in the overall synthesis of carbamoyl phosphate.

10 e intermediate during the final formation of carbamoyl phosphate.

11 re diminished in their ability to synthesize carbamoyl phosphate.

12 is attributed to phosphorylethanolamine, not carbamoyl phosphate.

13 mino group of Asp and the carbonyl carbon of carbamoyl phosphate.

14 rmational change induced upon the binding of carbamoyl phosphate.

15 le to utilize glutamine for the synthesis of carbamoyl phosphate.

16 ammonia for the ATP-dependent generation of carbamoyl phosphate.

17 cluding acetyl phosphate, benzoyl phosphate, carbamoyl phosphate, 2-methoxybenzoyl phosphate, and pho

18 A saturating concentration of carbamoyl phosphate alone has little influence on the sm

19 nit contains an N-terminal domain that binds carbamoyl phosphate and a C-terminal domain that binds L

20 vity and decreased substrate affinity toward carbamoyl phosphate and aspartate compared to the corres

21 ropic cooperativity, and the binding of both carbamoyl phosphate and aspartate were extremely comprom

22 little change in the Km for the substrates, carbamoyl phosphate and aspartate.

23 n mechanism is committed to the formation of carbamoyl phosphate and is not subject to hydrolysis.

24 ing established that the natural substrates, carbamoyl phosphate and L-aspartate, do not induce in th

25 identify the enzymatic synthesis of N-amino carbamoyl phosphate and N-hydroxy carbamoyl phosphate fr

26 lts in impaired synthesis of citrulline from carbamoyl phosphate and ornithine.

27 t catalyzes the synthesis of citrulline from carbamoyl phosphate and ornithine.

28 of the mutants were unable to synthesize any carbamoyl phosphate and the rest were severely crippled.

29 of ligands, in the presence of the substrate carbamoyl phosphate, and in the presence of the bisubstr

30 xaloacetate, the phosphorylation of MgADP by carbamoyl phosphate, and the bicarbonate-dependent ATPas

31 absence and presence of the first substrate carbamoyl phosphate are reported.

32 less negatively charged than its precursors, carbamoyl phosphate, aspartate, or carbamoyl aspartate.

33 in proteins which were unable to synthesize carbamoyl phosphate at a significant rate.

34 There is no preferential partitioning of carbamoyl phosphate between the arginine and pyrimidine

35 polypeptide chain folds into two domains, a carbamoyl phosphate binding domain and an L-ornithine bi

36 lex with citric acid bound in the postulated carbamoyl phosphate binding site, was determined in two

37 ghly conserved residue that is essential for carbamoyl-phosphate binding.

38 y two long interdomain helices: the putative carbamoyl phosphate-binding domain and a binding domain

39 The putative carbamoyl phosphate-binding site is similar to those in

40 l change that interferes with the binding of carbamoyl phosphate but has little effect once carbamoyl

41 bsaturating amounts of PALA or succinate and carbamoyl phosphate) caused a hyperbolic increase and de

42 rococcus abyssi demonstrate the existence of carbamoyl phosphate channeling in both the pyrimidine an

43 following the ATP synthesis reaction at low carbamoyl phosphate concentration.

44 topped-flow experiments, using aspartate and carbamoyl phosphate, confirm that the change in excimer

45 ucleotide biosynthesis, the reaction between carbamoyl phosphate (CP) and l-aspartate to form N-carba

46 responds to the position of the phosphate of carbamoyl phosphate (CP) and the position of the phospho

47 is, we identified acetyl phosphate (AcP) and carbamoyl phosphate (CP) as natural in vivo phosphodonor

48 Carbamoyl phosphate (CP) has a half-life for thermal dec

49 st, the bicarbonate-dependent ATPase and the carbamoyl phosphate-dependent ATP synthetase activities

50 The carboxy phosphate (residues 1-400) and carbamoyl phosphate domains (residues 553-933) also cont

51 e switch mechanism includes the synthesis of carbamoyl phosphate entirely within a single nucleotide

52 mation of MgATP and carbamate from MgADP and carbamoyl phosphate, Escherichia coli carbamoyl phosphat

53 e the absolute requirement of the binding of carbamoyl phosphate for the creation of the high-affinit

54 oyl-phosphate synthetase 1 activity produces carbamoyl phosphate for urea synthesis, and deficiency r

55 t catalyzes glutamine-dependent formation of carbamoyl phosphate for urea synthesis.

56 The absence of an initial burst of carbamoyl phosphate formation eliminates product release

57 of the partial reactions, the diminution of carbamoyl phosphate formation, and the percentage of the

58 Escherichia coli catalyzes the formation of carbamoyl phosphate from 2 mol of ATP, bicarbonate, and

59 a 120-kDa synthetase domain (CPS) that makes carbamoyl phosphate from ATP, bicarbonate, and ammonia u

60 of CPS.A and CPS.B proteins that synthesized carbamoyl phosphate from ATP, bicarbonate, and ammonia.

61 Escherichia coli catalyzes the formation of carbamoyl phosphate from bicarbonate, glutamine, and two

62 Escherichia coli catalyzes the formation of carbamoyl phosphate from bicarbonate, glutamine, and two

63 synthetase (CPS) catalyzes the formation of carbamoyl phosphate from bicarbonate, glutamine, and two

64 phate synthetase catalyzes the production of carbamoyl phosphate from bicarbonate, glutamine, and two

65 synthetase (CPS) catalyzes the formation of carbamoyl phosphate from glutamine, bicarbonate, and 2 m

66 of N-amino carbamoyl phosphate and N-hydroxy carbamoyl phosphate from hydroxylamine and hydrazine.

67 y 118 kDa) and catalyzes the biosynthesis of carbamoyl phosphate from MgATP, bicarbonate, and glutami

68 channeling ensures the efficient transfer of carbamoyl phosphate from the active site of CPSase to th

69 Escherichia coli catalyzes the production of carbamoyl phosphate from two molecules of Mg2+ATP, one m

70 Escherichia coli catalyzes the formation of carbamoyl phosphate from two molecules of MgATP, bicarbo

71 gh rapidly degraded at high temperature, the carbamoyl phosphate generated in situ by A. aeolicus car

72 nithine which attacks the carbonyl carbon of carbamoyl phosphate in the enzyme-catalyzed reaction.

73 hosphate synthetase-1 (CPS1), which produces carbamoyl phosphate in the mitochondria from ammonia and

74 d-type enzyme is required for the binding of carbamoyl phosphate in the proper orientation so as to i

75 led reaction, the effective concentration of carbamoyl phosphate in the vicinity of the ATCase active

76 must perform during the overall synthesis of carbamoyl phosphate in the wild type enzyme and the spec

77 vatization method revealed that the level of carbamoyl phosphate in these NSCLC extracts is below the

78 icarbonate during the enzymatic synthesis of carbamoyl phosphate, indicating that any carbon-containi

79 ontrast to the wild-type enzyme, addition of carbamoyl phosphate induced a significant alteration in

80 nally, the data indicate that the binding of carbamoyl phosphate induces conformational changes that

81 rbamoyl phosphate but has little effect once carbamoyl phosphate is bound.

82 miting step for the steady-state assembly of carbamoyl phosphate is either the formation, migration,

83 uples the two partial reactions such that no carbamoyl phosphate is produced.

84 the catalytic cysteine residue, can generate carbamoyl phosphate only in the presence of free ammonia

85 ng the altered CAD with the ATCase substrate carbamoyl phosphate or the bisubstrate analogue N-phosph

86 , is redirected for generating aspartate and carbamoyl phosphate periportally, and glutamine pericent

87 action catalyzed by biotin carboxylase where carbamoyl phosphate reacts with ADP was decreased 100-fo

88 the catalytic activity for the synthesis of carbamoyl phosphate relative to the wild type CPS, respe

89 dual partial reactions, overall synthesis of carbamoyl phosphate required a homodimer of CPS.A or CPS

90 nfirmed the hypothesis that the synthesis of carbamoyl phosphate requires the concerted action of the

91 ing agents, only AcP, and to a lesser extent carbamoyl phosphate, showed any significant phosphorylat

92 Vaccination with the carbamoyl phosphate synthase (CPS) mutant strain of Toxo

93 ioxin resulted in concomitant recruitment of carbamoyl phosphate synthase 1 (CPS1) to the NC-XRE.

94 ed to increase the level of succinylation on carbamoyl phosphate synthase 1, which is a known target

95 erence-mediated knockdown of a mitochondrial carbamoyl phosphate synthase impairs the response of nit

96 acetylglutamate, the obligatory activator of carbamoyl phosphate synthase-1 (CPS1).

97 ammonemia from reduced expression of hepatic carbamoyl phosphate synthase-I.

98 arbamoylases, and an internal duplication in carbamoyl phosphate synthase.

99 minases is demonstrated for Escherichia coli carbamoyl phosphate synthase.

100 Further analysis reveals carbamoyl-phosphate synthase 1 (CPS1) and sucrase-isomal

101 he lead variant on 2q24 (rs715) localizes to carbamoyl-phosphate synthase 1 (CPS1), which encodes a m

102 y facilitating pyrimidine synthesis via CAD (carbamoyl-phosphate synthase 2, aspartate transcarbamyla

103 xpression profile of the c-Myc target genes, carbamoyl-phosphate synthase-aspartate carbamoyltransfer

104 bited complex I of the respiratory chain and carbamoyl-phosphate synthase-I (CPS-I), with an EC(50) a

105 Carbamoyl-phosphate synthase/aspartate carbamoyltransfer

106 The partial recovery of carbamoyl phosphate synthesis activity in the double mut

107 in binding the two ATP molecules needed for carbamoyl phosphate synthesis and a carboxyl-terminal do

108 alues of K(m) for glutamine, but the overall carbamoyl phosphate synthesis reaction is unperturbed.

109 Carbamoyl phosphate synthesis requires the concerted act

110 tation, the rate of glutamine hydrolysis and carbamoyl phosphate synthesis were no longer coordinated

111 d HCO(3)(-), the other substrates needed for carbamoyl phosphate synthesis, bind to the synthetase do

112 5L exhibited a substantially reduced rate of carbamoyl phosphate synthesis, but the rate of ATP turno

113 Ser(44) --> Ala mutant than that needed for carbamoyl phosphate synthesis.

114 circuit that phases glutamine hydrolysis and carbamoyl phosphate synthesis.

115 he separately cloned subdomains can catalyze carbamoyl phosphate synthesis.

116 ifferent ATP-dependent reactions involved in carbamoyl phosphate synthesis.

117 valent and could each independently catalyze carbamoyl phosphate synthesis.

118 ATP-dependent partial reactions involved in carbamoyl phosphate synthesis.

119 hree reactions involved in ammonia-dependent carbamoyl phosphate synthesis.

120 ne or free ammonia as the nitrogen donor for carbamoyl phosphate synthesis.

121 ation of ATP bound to domain C is coupled to carbamoyl-phosphate synthesis at domain B via a nucleoti

122 imiting step in this pathway is catalysed by carbamoyl phosphate synthetase (CPS II), part of the mul

123 ein (MAP-2), myosin light chain (MYL-1), and carbamoyl phosphate synthetase (CPS III).

124 DP and carbamoyl phosphate, Escherichia coli carbamoyl phosphate synthetase (CPS) binds MgADP with a

125 Carbamoyl phosphate synthetase (CPS) catalyzes the forma

126 Carbamoyl phosphate synthetase (CPS) catalyzes the forma

127 Carbamoyl phosphate synthetase (CPS) catalyzes the produ

128 analysis of the allosteric responsiveness of carbamoyl phosphate synthetase (CPS) from E. coli was pe

129 The X-ray crystal structure of carbamoyl phosphate synthetase (CPS) from Escherichia co

130 Carbamoyl phosphate synthetase (CPS) from Escherichia co

131 Carbamoyl phosphate synthetase (CPS) from Escherichia co

132 The heterodimeric carbamoyl phosphate synthetase (CPS) from Escherichia co

133 Carbamoyl phosphate synthetase (CPS) from Escherichia co

134 Carbamoyl phosphate synthetase (CPS) from Escherichia co

135 rt of carbamate through the large subunit of carbamoyl phosphate synthetase (CPS) from Escherichia co

136 Carbamoyl phosphate synthetase (CPS) from Escherichia co

137 Carbamoyl phosphate synthetase (CPS) from Escherichia co

138 Carbamoyl phosphate synthetase (CPS) is a member of the

139 Carbamoyl phosphate synthetase (CPS) plays a key role in

140 The transfer of ammonia in carbamoyl phosphate synthetase (CPS) was investigated by

141 st three enzymes in pyrimidine biosynthesis, carbamoyl phosphate synthetase (CPS), aspartate transcar

142 individual mutant lines deficient in either carbamoyl phosphate synthetase (CPS), the first enzyme i

143 ayed by this class of enzymes is provided by carbamoyl phosphate synthetase (CPS).

144 l phosphate generated in situ by A. aeolicus carbamoyl phosphate synthetase (CPSase) was channeled to

145 a large multifunctional protein that carries carbamoyl phosphate synthetase (CPSase), aspartate trans

146 Human carbamoyl phosphate synthetase (hCPS) has evolved critic

147 Carbamoyl phosphate synthetase 1 (CPS1) is a liver-speci

148 the mitochondrial matrix and interacts with carbamoyl phosphate synthetase 1 (CPS1), an enzyme, cata

149 ased the catalytic efficiency for ammonia of carbamoyl phosphate synthetase 1 (CPS1), the rate-limiti

150 Systematic optimization of a carbamoyl phosphate synthetase 1 derived, glutarylated p

151 ylates and activates a mitochondrial enzyme, carbamoyl phosphate synthetase 1, which mediates the fir

152 proteins 70 and 90 (HSP-70; HSP-90), and the carbamoyl phosphate synthetase 2/aspartate transcarbamyl

153 he inhibitor UTP and the activator PRPP, the carbamoyl phosphate synthetase activity is controlled by

154 the sequential action of MAPK and PKA on the carbamoyl phosphate synthetase activity of CAD.

155 Carbamoyl phosphate synthetase catalyzes the hydrolysis

156 Carbamoyl phosphate synthetase catalyzes the production

157 The carbamoyl phosphate synthetase domain of the multifuncti

158 Carbamoyl phosphate synthetase from E. coli catalyzes th

159 ribe the X-ray crystallographic structure of carbamoyl phosphate synthetase from E. coli in which His

160 Carbamoyl phosphate synthetase from Escherichia coli cat

161 Carbamoyl phosphate synthetase from Escherichia coli cat

162 x-ray crystal structure of the heterodimeric carbamoyl phosphate synthetase from Escherichia coli has

163 Carbamoyl phosphate synthetase I (CPSase I; EC 6.3.4.16)

164 arginine serum levels on chromosome 2 at the carbamoyl phosphate synthetase I locus, on chromosome 5

165 ine nucleotide synthesis is catalyzed by the carbamoyl phosphate synthetase II (CPSase) domain of CAD

166 Carbamoyl phosphate synthetase II (CPSII) is part of car

167 the virulence of T. gondii mutants that lack carbamoyl phosphate synthetase II (uracil auxotrophs) to

168 Carbamoyl phosphate synthetase is isolated from Escheric

169 mately 25 A in length, whereas the tunnel in carbamoyl phosphate synthetase is nearly 100 A long.

170 The arginine-specific carbamoyl phosphate synthetase of Saccharomyces cerevisi

171 , cytidine 5'-triphosphate (CTP) synthetase, carbamoyl phosphate synthetase, and phosphoribosyl pyrop

172 mensional structures of tryptophan synthase, carbamoyl phosphate synthetase, glutamine phosphoribosyl

173 n some cases, such as biotin carboxylase and carbamoyl phosphate synthetase, the B-domains move signi

174 r rs1047891, a functional variant located in carbamoyl phosphate synthetase-1 (CPS1) gene.

175 KL cells express the urea cycle enzyme carbamoyl phosphate synthetase-1 (CPS1), which produces

176 is similar to the folding of this domain in carbamoyl phosphate synthetase.

177 ransferase type I domain of Escherichia coli carbamoyl phosphate synthetase.

178 r the tunneling of ammonia within the native carbamoyl phosphate synthetase.

179 specifying the small subunit of Arg-specific carbamoyl phosphate synthetase.

180 codes the small subunit of arginine-specific carbamoyl phosphate synthetase.

181 the G359F (small subunit) mutant protein of carbamoyl phosphate synthetase.

182 ned a 240 kDa protein that was identified as carbamoyl phosphate synthetase/aspartate transcarbamoyla

183 l phosphate synthetase II (CPSII) is part of carbamoyl phosphate synthetase/aspartate transcarbamoyla

184 Carbamoyl phosphate synthetase/aspartate transcarbamylas

185 Carbamoyl-phosphate synthetase (CPS) from Escherichia co

186 he kinetics of the coupled reactions between carbamoyl-phosphate synthetase (CPSase) and both asparta

187 Carbamoyl-phosphate synthetase (CPSase) consists of a 12

188 Escherichia coli carbamoyl-phosphate synthetase (CPSase) is comprised of

189 hyperthermophile, has neither a full-length carbamoyl-phosphate synthetase (CPSase) resembling the e

190 On the contrary, carbamoyl-phosphate synthetase 1 (CPS1), ALB, the prolif

191 In mitochondria, carbamoyl-phosphate synthetase 1 activity produces carba

192 a neonate had received a diagnosis of severe carbamoyl-phosphate synthetase 1 deficiency, a disease w

193 te membrane putative receptor (identified as carbamoyl-phosphate synthetase 1).

194 The tri-functional enzyme contains carbamoyl-phosphate synthetase 2 (CPS2), aspartate trans

195 which directly phosphorylates S1859 on CAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamo

196 Carbamoyl-phosphate synthetase 2, aspartate transcarbamo

197 Moreover, EGFR signaling activated carbamoyl-phosphate synthetase 2, aspartate transcarbamy

198 orylates the initial enzyme of this pathway, carbamoyl-phosphate synthetase 2, aspartate transcarbamy

199 hatidylinositol 4-kinases (PI4KA and PI4KB), carbamoyl-phosphate synthetase 2, aspartate transcarbamy

200 Cytoplasmic carbamoyl-phosphate synthetase 2, however, is part of a

201 uted to altered allosteric regulation of the carbamoyl-phosphate synthetase activity of CAD (carbamoy

202 Carbamoyl-phosphate synthetase catalyzes the production

203 Carbamoyl-phosphate synthetase consists of an amidotrans

204 oncerted action of the glutaminase (GLN) and carbamoyl-phosphate synthetase domains of CAD.

205 The frequencies of the carbamoyl-phosphate synthetase genotypes in the study po

206 Carbamoyl-phosphate synthetase I (CPSase I) catalyzes th

207 ferential plasma proteins detected by iTRAQ, carbamoyl-phosphate synthetase I (CPSI, related to urea

208 d enzymes ornithine carbamoyltransferase and carbamoyl-phosphate synthetase III (CPSase III) are indu

209 Carbamoyl-phosphate synthetase III (CPSase III) of Squal

210 the first enzyme in the urea cycle pathway, carbamoyl-phosphate synthetase III (CPSase III), is too

211 Mammalian carbamoyl-phosphate synthetase is part of carbamoyl-phos

212 this closed form of biotin carboxylase with carbamoyl-phosphate synthetase is presented.

213 Ser(44) GLN domain and the Escherichia coli carbamoyl-phosphate synthetase large subunit had little

214 skewed distribution of the genotypes for the carbamoyl-phosphate synthetase variants at position 1405

215 trations of amino acids and genotypes of the carbamoyl-phosphate synthetase variants were determined

216 SARS-CoV-2 deploys Nsp9 to activate carbamoyl-phosphate synthetase, aspartate transcarbamoyl

217 e thioester intermediate of Escherichia coli carbamoyl-phosphate synthetase, indicates that the subst

218 for threonine at position 1405 [T1405N]) in carbamoyl-phosphate synthetase, which controls the rate-

219 urea cycle--in particular, the efficiency of carbamoyl-phosphate synthetase--may contribute to the av

220 an carbamoyl-phosphate synthetase is part of carbamoyl-phosphate synthetase-aspartate carbamoyltransf

221 bamoyl-phosphate synthetase activity of CAD (carbamoyl-phosphate synthetase-aspartate carbamoyltransf

222 d c-myc, dihydrofolate reductase (DHFR), and carbamoyl-phosphate synthetase-aspartate transcarbamoyl-

223 ession of mature hepatocytic markers such as carbamoyl-phosphate synthetase1 and several cytochrome P

224 oning was used to identify a mutation in the carbamoyl-phosphate synthetase2-aspartate transcarbamyla

225 Depending on their physiological role, carbamoyl phosphate synthetases (CPSs) use either glutam

226 Carbamoyl phosphate synthetases (CPSs) utilize either gl

227 Bacillus stearothermophilus contains two carbamoyl-phosphate synthetases (CPS), one specific for

228 Although carbamoyl-phosphate synthetases (CPSs) share sequence id

229 Carbamoyl-phosphate synthetases (CPSs) utilize two molec

230 The B-domain of the carbamoyl phosphate synthetic component of the large sub

231 lves referred to as the carboxyphosphate and carbamoyl phosphate synthetic components.

232 phate synthetase catalyzes the production of carbamoyl phosphate through a reaction mechanism requiri

233 tase from E. coli catalyzes the synthesis of carbamoyl phosphate through a series of four reactions o

234 ast step in this pathway, converting ADP and carbamoyl phosphate to ATP and ammonium carbamate.

235 inine dihydrolase pathway converting ADP and carbamoyl phosphate to ATP and carbamate.

236 The inability of carbamoyl phosphate to create the high-affinity binding

237 gue succinate, in the presence of saturating carbamoyl phosphate, to the pyrenelabeled enzyme caused

238 our other metabolites, S-adenosylmethionine, carbamoyl phosphate, UDP-glucose, and Delta(2)-isopenten

239 l-molecule phosphodonors acetyl phosphate or carbamoyl phosphate under conditions in which a control

240 he larger subunit catalyzes the formation of carbamoyl phosphate using 2 mol of ATP, bicarbonate, and

241 This mutant is unable to synthesize carbamoyl phosphate using glutamine as a nitrogen source

242 However, carbamoyl phosphate was able to shift the structure of t

243 teady-state time course for the formation of carbamoyl phosphate was linear with an overall rate cons

244 However, neither ammonia nor carbamoyl phosphate was produced, which implies that pur

245 formation of phosphate, ADP, glutamate, and carbamoyl phosphate were determined.

246 synthetase (CPS) catalyzes the production of carbamoyl phosphate which is subsequently employed in th

247 Escherichia coli catalyzes the formation of carbamoyl phosphate, which is subsequently employed in b