ライフサイエンスコーパス: carbapenemase

1 , particularly variants of the K. pneumoniae carbapenemase.

2 , 90 (47.9%; 95% CI, 40.6%-55.1%) produced a carbapenemase.

3 nsistent feature of strains producing OXA-48 carbapenemase.

4 umannii isolates producing acquired OXA-type carbapenemases.

5 Only BD Phoenix CPO Detect classified carbapenemases.

6 isolate collection (n = 48), with a range of carbapenemases.

7 bapenemase tests, and classification of most carbapenemases.

8 carbapenemases were misclassified as class A carbapenemases.

9 sults across multiple sites for detection of carbapenemases.

10 cing P. aeruginosa isolates produced class B carbapenemases.

11 amily Enterobacteriaceae that do not produce carbapenemases.

12 id, accurate detection and classification of carbapenemases.

13 ss A, 72.4% of class B, and 88.6% of class D carbapenemases.

14 97.6%) and 99.0% (94.4%, 99.8%) for class D carbapenemases.

15 solates were included, of which 29% produced carbapenemases.

16 teriaceae that produce Klebsiella pneumoniae carbapenemases.

17 positive for OXA-48, KPC, NDM, VIM, and IMP carbapenemases.

18 ing extended-spectrum beta-lactamases and/or carbapenemases.

19 stance mechanisms, and stability against all carbapenemases.

20 isolates, 73.2% (52) produced K. pneumoniae carbapenemases-2 (KPC-2).

21 most frequent carbapenemase was K pneumoniae carbapenemase (329 [75%]; 253 [74%] vs 76 [81%]).

22 sp. isolate producing Klebsiella pneumoniae Carbapenemase-4 and New Delhi Metallo-beta-Lactamase-1 i

23 lla pneumoniae and 74 harbored K. pneumoniae carbapenemase (56.1%), 54 metallo-beta-lactamase (40.9%)

24 We demonstrate that carbapenemase acquisition is the main cause of carbapene

25 All isolates containing an OXA-48 family carbapenemase across all three test sets were correctly

26 d specificity of meropenem and ertapenem for carbapenemase activity among non-Enterobacteriaceae were

27 Current methods to detect carbapenemase activity are suboptimal, requiring prolong

28 vation method (mCIM) was developed to detect carbapenemase activity directly from positive blood cult

29 g and selected reaction monitoring to detect carbapenemase activity from pathogenic microorganisms in

30 accurate, objective phenotypic detection of carbapenemase activity in Enterobacteriaceae In this iss

31 ity test using faropenem (10 mug) to predict carbapenemase activity in Enterobacteriaceae.

32 ility but does not play a direct role in the carbapenemase activity of the GES family of beta-lactama

33 tive and specific assay for the detection of carbapenemase activity using ertapenem and liquid chroma

34 ed disulfide bridge is responsible for their carbapenemase activity, but this has not yet been valida

35 eta-lactamases (MBLs), especially those with carbapenemase activity, threaten the clinical utility of

36 ion by KPC-2 and its unique cephalosporinase/carbapenemase activity.

37 d cephalosporinase activity, as well as weak carbapenemase activity.

38 ead of extended-spectrum beta-lactamases and carbapenemases among common bacterial pathogens are thre

39 ass D carbapenemases were the most prevalent carbapenemases among the carbapenemase-producing A. baum

40 ative pathogens that produce a transmissible carbapenemase and are typically resistant to most (somet

41 ity against Gram-negative bacteria including carbapenemase and carbacephalosporinase producing strain

42 OXA-48 is a highly prevalent carbapenemase and has been isolated worldwide.

43 ly described limitations with blaOXA-48-like carbapenemases and blaOXA carbapenemases associated with

44 ended-Spectrum BLs (ESBL), KPC- and OXA-type carbapenemases and metallo-beta-lactamases (MBL).

45 Carbapenemases and other types of antibiotic resistance

46 ICT) for the detection of OXA-48/OXA-48-like carbapenemases and the development of an algorithm for r

47 Six isolates produced two different carbapenemases, and 43 carbapenemase-negative isolates w

48 n-bonding networks in NMCA, SFC-1, and SME-1 carbapenemases are less intensive, and as a consequence,

49 OXA-48 has emerged as a major carbapenemase associated with the Enterobacteriaceae in

50 ith blaOXA-48-like carbapenemases and blaOXA carbapenemases associated with Acinetobacter baumannii w

51 xtended-spectrum beta-lactamases (ESBLs) and carbapenemases belonging to different molecular classes:

52 s residue is conserved in both KPC-2 and non-carbapenemase beta-lactamases, suggesting it promotes ca

53 esistance mechanisms were KPC (K. pneumoniae carbapenemases) beta-lactamases encoded by blaKPC2, blaK

54 2010-2012, when the now globally-distributed carbapenemase bla-NDM-1 was being acquired by Klebsiella

55 CPOs carrying the Klebsiella pneumoniae carbapenemase (bla KPC ) gene have caused outbreaks glob

56 Isolates carrying genes encoding carbapenemases (bla (KPC-2,) bla (KPC-3), and bla (NDM-1

57 reads characterized as beta-lactamases, the carbapenemase blaOXA was dominant in most of the effluen

58 ruption of the disulfide bridge in the GES-5 carbapenemase by the C69G substitution results in only m

59 ae expressing IMP, VIM, KPC, NDM, and/or OXA carbapenemases, by using imipenem, meropenem, and ertape

60 The PPA values for carbapenemase class designations for all organisms combi

61 Carbapenemase classes tested include VIM, IMP, NDM, SPM,

62 Culture of a PCR-confirmed, carbapenemase-containing organism, or history of coloniz

63 The observed stereoselectivity implies that carbapenemases control the form of their pyrroline ring

64 Both tests exhibited high sensitivity of carbapenemase detection (>97%).

65 We compared carbapenemase detection among 271 Gram-negative bacilli

66 d a method comparison study of 11 phenotypic carbapenemase detection assays to evaluate their accurac

67 clinical performance of Check-Direct CPE for carbapenemase detection directly from 301 perirectal swa

68 ce areas or in outbreak settings where rapid carbapenemase detection is critical for infection contro

69 The overall sensitivity for carbapenemase detection was 96.4% (95% confidence interv

70 provides novel advantages such as automated carbapenemase detection, inclusion in susceptibility pan

71 atory in parallel with molecular methods for carbapenemase detection.

72 niae (KP) sequence type ST258 is mediated by carbapenemases (e.g. KPC-2) and loss or modification of

73 Weakly hydrolyzing carbapenemases (e.g., OXA-48-like) were also well detect

74 rentiate between serine- and metal-dependent carbapenemases elaborated by carbapenemase-producing iso

75 A carbapenemase-encoding gene was found in 81.7% (94/115)

76 We describe the phenotype, clonality, and carbapenemase-encoding genes present in CRE isolated fro

77 1)) were also long-read sequenced, and their carbapenemase-encoding plasmid sequences were compared w

78 are genetically diverse and that CNSC harbor carbapenemase-encoding plasmids found in other Enterobac

79 GNB) from positive blood cultures and all 14 carbapenemase enzymes tested.

80 goal of finding a potent inhibitor of serine carbapenemase enzymes that are currently compromising th

81 detection and differentiation of five common carbapenemase families (KPC, OXA-48-like, VIM, IMP, and

82 tely detected and differentiated five common carbapenemase families from Enterobacterales and P. aeru

83 and the ability to confer resistance, within carbapenemase families.

84 racteristics of chromID CARBA and HardyCHROM Carbapenemase for the detection of carbapenemase-produci

85 lo-beta-lactamase (VIM) were the most common carbapenemases found.

86 od for the direct detection of OXA-48 family carbapenemases from cultured isolates that may have util

87 ng identified 1,493 isolates that harbored a carbapenemase gene (1,485 ertapenem-nonsusceptible isola

88 Detection of a carbapenemase gene at a C(T) cutoff value of </=35 was c

89 stin-resistant genes, mcr-1.1 and mcr-3.5, a carbapenemase gene bla(NDM-5), and a 16S methylase gene

90 bla NDM-1 was the only carbapenemase gene detected.

91 n direct PCR (with culture of specimens if a carbapenemase gene was detected) replaced culture.

92 ase, we retrospectively tracked the bla(KPC) carbapenemase gene-bearing pKpQIL plasmid responsible fo

93 Neither method detected the bla (OXA-48) carbapenemase gene.

94 ively detects and differentiates five common carbapenemase genes (bla (KPC), bla (NDM), bla (VIM), bl

95 R (qPCR) assay that detects five families of carbapenemase genes (blaIMP, blaKPC, blaNDM, blaOXA-48,

96 -lactamase (bla) NDM, VIM, IMP, KPC, and OXA carbapenemase genes (e.g., blaOXA-23, blaOXA-24/40, and

97 -lactamase (bla) NDM, VIM, IMP, KPC, and OXA carbapenemase genes (e.g., blaOXA-23, blaOXA-24/40, and

98 Thus, the acquisition of carbapenemase genes by these organisms increases the ris

99 Rapid methods for tracking plasmids carrying carbapenemase genes could greatly benefit infection cont

100 R assay for detection and differentiation of carbapenemase genes from sputum specimens in patients wi

101 ocols might not correctly reflect the HGT of carbapenemase genes in vivo.

102 to carbapenems in vitro for the presence of carbapenemase genes remains controversial and requires f

103 ype were assessed for the presence of common carbapenemase genes using a Check-MDR CT101 microarray (

104 Carbapenemase genes were detected by polymerase chain re

105 Carbapenemase genes were detected by the Carba-R assay i

106 Carbapenemase genes were most frequently identified in K

107 argeted PCR as to the presence or absence of carbapenemase genes were tested for carbapenemase produc

108 obacteriaceae The most frequently identified carbapenemase genes were the KPC (n = 794), OXA-48-like

109 with polymerase chain reaction analysis for carbapenemase genes, and isolates with the blaOXA-232 ge

110 ant source of community exposure to ESBL and carbapenemase genes, and that these genes may be dissemi

111 ged to sequence type (ST) 88 and carried two carbapenemase genes, bla(KPC-18) and bla(VIM-1).

112 is frequently encoded on the same plasmid as carbapenemase genes, ensures that propylamycin will not

113 pe bla(SHV) or bla(TEM), or bla(OXA-48)-type carbapenemase genes, including at least one positive sam

114 e contrasting modes of dissemination used by carbapenemase genes, which confer resistance to last-lin

115 tured to isolate organisms harboring ESBL or carbapenemase genes.

116 at (i) 60% of the isolates harbored multiple carbapenemase genes; (ii) the blaDIM-1 gene, which has p

117 This would likely apply to all other class A carbapenemases given the high degree of their structural

118 tion of metallo-beta-lactamase with KPC-type carbapenemase has implications for the use of next-gener

119 However, the emergence of the KPC-2 carbapenemase has resulted in widespread resistance to t

120 er but still insufficient, because different carbapenemases have differing treatment implications, pa

121 henotypic methods exist for the detection of carbapenemases; however, clinical laboratories have stru

122 from carbapenem-nonsusceptible isolates for carbapenemase identification.

123 OXA-48 is the most prevalent carbapenemase in Enterobacteriaceae in Europe and the Mi

124 O detect (CPO detect) detects and classifies carbapenemases in Enterobacterales, Acinetobacter bauman

125 The increasing dissemination of carbapenemases in Gram-negative bacteria has threatened

126 ssay for the identification of OXA-48 family carbapenemases in subcultured bacterial isolates based o

127 n included 87 isolates that produced class A carbapenemases (including KPC-2, -3, -4, -5, -6, and -8,

128 ity and specificity for the five most common carbapenemases, including IMP variants.

129 tential for use in the specific detection of carbapenemases, including metallo-beta-lactamases in act

130 ded 133 CPE strains producing a total of 139 carbapenemases, including VIM (n = 48), OXA-48-like (n =

131 pectrum beta-lactam resistance genes such as carbapenemases is detrimental to the use of antibiotics

132 enzymes, collectively referred to as class A carbapenemases, is a disulfide bridge between invariant

133 vitro activity against Klebsiella pneumoniae carbapenemase (KPC) enzyme producers, but clinical trial

134 neumoniae strains that produce K. pneumoniae carbapenemase (KPC) have spread globally in the last dec

135 m (ETP) resistance and Klebsiella pneumoniae carbapenemase (KPC) in 47 Klebsiella pneumoniae isolates

136 Klebsiella pneumoniae carbapenemase (KPC) is a widespread SBL that hydrolyzes

137 nfirmatory testing for Klebsiella pneumoniae carbapenemase (KPC) production or other beta-lactamases

138 Imipenemase (IMP), Klebsiella pneumoniae carbapenemase (KPC), and Verona integron-encoded metallo

139 bla KPC, encoding a transmissible carbapenemase (KPC), has historically largely been assoc

140 ases, particularly the Klebsiella pneumoniae carbapenemase (KPC), with no inhibition of mammalian ser

141 wo clinical strains of Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae were studied

142 pread dissemination of Klebsiella pneumoniae carbapenemase (KPC).

143 ples and 77 (19%) of 402 E coli samples were carbapenemase (KPC, NDM, OXA-48-like, or VIM) producers.

144 the production of the Klebsiella pneumoniae carbapenemase (KPC-2) class A beta-lactamase.

145 ted colonization with a multidrug-resistant, carbapenemase (KPC-3)-producing Klebsiella pneumoniae is

146 profiles, whereas all Klebsiella pneumoniae carbapenemase (KPC; n = 8) and GES (n = 12) isolates tes

147 s to identify specific Klebsiella pneumoniae carbapenemases (KPC) and additional beta-lactamases.

148 llo-beta-lactamases or Klebsiella pneumoniae carbapenemases (KPC), no specific inhibitor is available

149 Klebsiella pneumoniae carbapenemases (KPCs) were first identified in 1996 in t

150 e of enhanced diagnostic tests for detecting carbapenemases locally and nationally.

151 nals, and regulatory agencies to specify the carbapenemases meant.

152 bapenemases (n = 84) or Ambler class A and B carbapenemases (n = 41) and carbapenemase-negative isola

153 luding isolates producing OXA-48/OXA-48-like carbapenemases (n = 84) or Ambler class A and B carbapen

154 The four most common carbapenemases (NDM, KPC, OXA-48-like, and VIM) were det

155 er class A and B carbapenemases (n = 41) and carbapenemase-negative isolates (n = 41).

156 The challenge collection included 81 carbapenemase-negative isolates and 104 CPEs (OXA-48 [n

157 and 232 were produced by 243 isolates and 51 carbapenemase-negative isolates included porin mutants a

158 roduced two different carbapenemases, and 43 carbapenemase-negative isolates were included as negativ

159 Due to the highly challenging carbapenemase-negative isolates, specificities were lowe

160 Non-carbapenemase (non-CP)-harboring isolates were also test

161 mCIM; isolates with Ambler class A, B, and D carbapenemases, non-CP-CRE isolates, and carbapenem-susc

162 stigated the impact of ancillary testing for carbapenemase of isolates that met the CDC CPE surveilla

163 inhibitor-resistant beta-lactamase enzymes, carbapenemases or ampC type beta-lactamases, at least on

164 Gram-negative pathogens that produce ESBLs, carbapenemases or multiple beta-lactamases in the same o

165 d 91% (112/123) of P. aeruginosa isolates as carbapenemases or non-carbapenemase producers, with disc

166 Outer membrane proteins OmpA and YiaD, and carbapenemase Oxa-23 are hubs of the identified interact

167 The probe binds the class D carbapenemase OXA-24/40 with close to the same affinity

168 variants, including the clinically observed carbapenemase OXA-48 V120L, supports the proposal that c

169 on of the blaOXA48 gene, which codes for the carbapenemase OXA-48, in lysate samples from Klebsiella

170 is on the identification of beta-lactamases (carbapenemases OXA-48 and KPC in particular) in bacteria

171 hic lateral flow assay to detect OXA-48-like carbapenemases (OXA-48 K-SeT) in Enterobacteriaceae (n =

172 CRE are increasing in California, and carbapenemases, particularly KPC, are a common mechanism

173 identified with potent inhibition of serine carbapenemases, particularly the Klebsiella pneumoniae c

174 performance of this definition, compared to carbapenemase PCR, for a collection of 125 Enterobacteri

175 the presence of OXA-48 and KPC in all of the carbapenemase positive samples, independent of species a

176 ceptible to imipenem were not uncommon among carbapenemase-positive isolates (9.4%, 141/1,493) and mo

177 However, 477 of 682 (69.9%) carbapenemase-positive isolates are concentrated in four

178 lates with the degree of resistance and that carbapenemase-positive isolates have the highest transmi

179 over half of the hospitals that contributed carbapenemase-positive isolates probably experienced wit

180 Among the carbapenemase-positive isolates, 66.7% (2/3), 37.0% (111

181 ear after a Guiana Extended-Spectrum (GES)-5 carbapenemase-positive Klebsiella oxytoca infection was

182 One year after a GES-5 carbapenemase-positive Klebsiella oxytoca infection was

183 The growing prevalence and diversity of carbapenemase producers among carbapenem-resistant Pseud

184 % to 100%) by the two tests, with all double carbapenemase producers being correctly detected by both

185 271 Gram-negative bacilli (of which 131 were carbapenemase producers) using a novel chromogenic rapid

186 aeruginosa isolates as carbapenemases or non-carbapenemase producers, with discordant isolates being

187 m-negative bacillary isolates, including 134 carbapenemase producers.

188 alysed in 40 epidemiologically-related NDM-1 carbapenemase producing Klebsiella pneumoniae isolates i

189 Carbapenemase producing organisms (CPOs) represent an ur

190 Multidrug resistant (MDR) carbapenemase-producing (CP) Klebsiella pneumoniae, belo

191 aring 14-day mortality between patients with carbapenemase-producing (CP)-CRE compared with non-CP-CR

192 CP isolates (Ambler class A, B, and D), non-carbapenemase-producing (non-CP) carbapenem-resistant is

193 ducing A. baumannii strains, with 60% of the carbapenemase-producing A. baumannii isolates producing

194 antibiotic efficacy in OXA-23 and OXA-24/40 carbapenemase-producing A. baumannii strains (1 mug mL(-

195 the most prevalent carbapenemases among the carbapenemase-producing A. baumannii strains, with 60% o

196 ays achieved >90% specificity in identifying carbapenemase-producing A. baumannii, no assays achieved

197 assays may be necessary to accurately detect carbapenemase-producing A. baumannii.

198 producing Pseudomonas aeruginosa (CP-PA) and carbapenemase-producing Acinetobacter baumannii (CP-AB)

199 esistance requires the consideration of both carbapenemase-producing bacteria as well as bacteria wit

200 Detecting colonization of patients with carbapenemase-producing bacteria can be difficult.

201 we analyzed nucleic acids extracted from 128 carbapenemase-producing bacteria isolated from clinical

202 microbiology laboratories to reliably detect carbapenemase-producing carbapenem-resistant Enterobacte

203 The emergence and spread of carbapenemase-producing carbapenem-resistant Enterobacte

204 95% CI: 1.10-7.41) were associated with non-carbapenemase-producing CRE (NCPE) (n = 88) compared wit

205 Early identification of infections caused by carbapenemase-producing Enterobacterales (CPE) can help

206 n-based programs for control of MROs such as Carbapenemase-producing Enterobacterales (CPE) have emer

207 Data on household transmission of carbapenemase-producing Enterobacterales (CPE) remain li

208 the Xpert Carba-R assay for the detection of carbapenemase-producing Enterobacterales (CPE) strains.

209 A collection of 166 isolates of carbapenemase-producing Enterobacteriaceae (CPE) and 82

210 formation that enhances our understanding of carbapenemase-producing Enterobacteriaceae (CPE) and whi

211 Carbapenemase-producing Enterobacteriaceae (CPE) are a s

212 Carbapenemase-producing Enterobacteriaceae (CPE) are eme

213 ully reduced colonization and infection with carbapenemase-producing Enterobacteriaceae (CPE) in Chic

214 last 2 decades, but global dissemination of carbapenemase-producing Enterobacteriaceae (CPE) is a mo

215 Early identification of carbapenemase-producing Enterobacteriaceae (CPE) is esse

216 The best available treatment against carbapenemase-producing Enterobacteriaceae (CPE) is unkn

217 klin Lakes, NJ) inoculated with 27 different carbapenemase-producing Enterobacteriaceae (CPE) isolate

218 Rapid detection of carbapenemase-producing Enterobacteriaceae (CPE) represe

219 on antimicrobial agent with activity against carbapenemase-producing Enterobacteriaceae (CPE) with me

220 , 108 (43%) were female, and 161 (64.7%) had carbapenemase-producing Enterobacteriaceae (CPE).

221 ardyCHROM Carbapenemase for the detection of carbapenemase-producing Enterobacteriaceae (CPE).

222 All specimens were analyzed for ESBL- and carbapenemase-producing Enterobacteriaceae (CPE).

223 Klebsiella pneumoniae carbapenemase-producing Enterobacteriaceae (hereafter "K

224 Carbapenemase-producing Enterobacteriaceae are an emergi

225 Infections caused by carbapenemase-producing Enterobacteriaceae are increasin

226 Here, we quantified the effects of carbapenemase-producing Enterobacteriaceae carriage on p

227 Carbapenemase-producing Enterobacteriaceae colonization

228 tios and subdistribution hazard ratios, with carbapenemase-producing Enterobacteriaceae colonization

229 lonized at admission, and 96 (9.5%) acquired carbapenemase-producing Enterobacteriaceae colonization

230 Carbapenemase-producing Enterobacteriaceae colonization

231 ge on patient outcome in two Greek ICUs with carbapenemase-producing Enterobacteriaceae endemicity.

232 extended-spectrum beta-lactamase- (ESBL) and carbapenemase-producing Enterobacteriaceae has significa

233 Patients colonized with carbapenemase-producing Enterobacteriaceae have on avera

234 Although carbapenemase-producing Enterobacteriaceae have received

235 revalence and impact of infections caused by carbapenemase-producing Enterobacteriaceae is a global h

236 Of 132 carbapenemase-producing Enterobacteriaceae isolates, 125

237 n 32 countries during the European Survey of Carbapenemase-Producing Enterobacteriaceae.

238 dards in Europe make it difficult to contain carbapenemase-producing Enterobacteriaceae.

239 Three travelers (0.5%) acquired carbapenemase-producing Enterobacteriaceae.

240 , which account for 97% of the UK's reported carbapenemase-producing Enterobacteriaceae.

241 nent-wide enhanced sentinel surveillance for carbapenemase-producing Enterobacteriaeceae can be overc

242 nent-wide enhanced sentinel surveillance for carbapenemase-producing Enterobacteriaeceae can be overc

243 Accurate detection of carbapenemase-producing glucose-nonfermenting Gram-negat

244 metal-dependent carbapenemases elaborated by carbapenemase-producing isolates for epidemiologic, infe

245 Carbapenemase-producing K pneumoniae isolates showed hig

246 ical impact of a novel Klebsiella pneumoniae carbapenemase-producing K. pneumoniae (KPC-KP) sequence

247 istant enterococci and Klebsiella pneumoniae carbapenemase-producing K. pneumoniae followed a similar

248 Carbapenemase-producing K. pneumoniae have become a glob

249 )) and also provided good results for OXA-48 carbapenemase-producing K. pneumoniae strains (4 mug mL(

250 issible CPE worldwide, Klebsiella pneumoniae carbapenemase-producing K. pneumoniae; and present strat

251 first structured survey on the occurrence of carbapenemase-producing Klebsiella pneumoniae and Escher

252 Carbapenemase-producing Klebsiella pneumoniae has become

253 New approaches for carbapenemase-producing organism (CPO) detection may hel

254 Carbapenemase-producing organisms (CPO) have been identi

255 ification of patients who are colonized with carbapenemase-producing organisms (CPO) is included in m

256 e for at least one CRO and included 213 (3%) carbapenemase-producing organisms (CPO), resulting in a

257 ost concerning trends is the rapid spread of Carbapenemase-Producing Organisms (CPO), where colistin

258 were the most sensitive for the detection of carbapenemase-producing organisms (CPOs) (100%; all blaK

259 dy of public health response to C. auris and carbapenemase-producing organisms (CPOs) at one ventilat

260 The accurate detection of carbapenemase-producing organisms is a major challenge f

261 id identification of patients colonized with carbapenemase-producing organisms using multiplex PCR ma

262 Carbapenemase-producing organisms, or CPOs, are Gram-neg

263 est, had specificities of >90% for detecting carbapenemase-producing P. aeruginosa Class D carbapenem

264 A total of 86% of the carbapenemase-producing P. aeruginosa isolates produced

265 nt sensitivity and specificity for detecting carbapenemase-producing P. aeruginosa isolates.

266 Identification of carbapenemase-producing P. aeruginosa will have therapeu

267 h a sensitivity of >90% for the detection of carbapenemase-producing P. aeruginosa, including all rap

268 e is threatened by the growing prevalence of carbapenemase-producing pathogens.

269 ctivation method (mCIM) for the detection of carbapenemase-producing Pseudomonas aeruginosa (CP-PA) a

270 oniae (hypermucoviscous K1, research K2, and carbapenemase-producing ST258) strains, the absence of C

271 0 mul was required for reliable detection of carbapenemase production among P. aeruginosa and A. baum

272 arba NP test to be accurate for detection of carbapenemase production among P. aeruginosa isolates an

273 s a phenotypic test that not only identifies carbapenemase production but also distinguishes between

274 this study, protocols for rapid detection of carbapenemase production directly from positive blood cu

275 ays required an increased inoculum to detect carbapenemase production in isolates with blaNDM, blaIMP

276 es between metallo-beta-lactamase and serine-carbapenemase production in P. aeruginosa The mCIM test

277 sence of carbapenemase genes were tested for carbapenemase production using the mCIM; isolates with A

278 tration and hemolysis step before a test for carbapenemase production was performed.

279 their performance of a test for detection of carbapenemase production, the Carba NP test.

280 cific phenotypic method for the detection of carbapenemase production, the carbapenem inactivation me

281 lineating procedures for identifying CRE and carbapenemase production.

282 ent cross-class inhibition of representative carbapenemases, specifically the SBL KPC-2 and the MBLs

283 nes by these organisms increases the risk of carbapenemase spread in general.

284 Carbapenemases such as KPC, NMC-A, IMI, SME, NDM, SPM, I

285 example is increasing multi-AR due to mobile carbapenemases, such as NDM-1 protein (coded by blaNDM-1

286 an alternative phenotypic test, the indirect carbapenemase test, for the detection of blaKPC-producin

287 imicrobial Susceptibility Testing agree that carbapenemase testing is not necessary for clinical care

288 ation on the development and modification of carbapenemase tests continues, as is the case in this is

289 minate delays and subjectivity in initiating carbapenemase tests, and classification of most carbapen

290 erally highlight the active site features of carbapenemases that can be leveraged for lead discovery.

291 ance upon carbapenems, but the expression of carbapenemases threatens to limit the utility of these d

292 inappropriate therapy) and the most frequent carbapenemase was K pneumoniae carbapenemase (329 [75%];

293 Detection of carbapenemases was performed phenotypically, with confir

294 ere reviewed and molecular typing for common carbapenemases was performed.

295 ns such as ceftazidime/avibactam, no class B carbapenemases were misclassified as class A carbapenema

296 arbapenemase-producing P. aeruginosa Class D carbapenemases were the most prevalent carbapenemases am

297 The most common carbapenemases were the OXA-23-type, found in 107 isolat

298 ndent (i.e., metallo-beta-lactamases [MBLs]) carbapenemases when used in conjunction with the modifie

299 y producing beta-lactamases (BLs), including carbapenemases, which are able to hydrolyze nearly all a

300 and laboratory bacterial strains expressing carbapenemases while showing some cytotoxicity toward hu