ライフサイエンスコーパス: cell culture

1 es and inhibits activity, which we verify in cell culture.

2 ism is supported by pull-down experiments in cell culture.

3 ding efficient drug liberation in plasma and cell culture.

4 with which to explore the effects of tau in cell culture.

5 yme activity in vitro and parasite growth in cell culture.

6 e potentially generated during production in cell culture.

7 hat do not compromise replicative fitness in cell culture.

8 ity to LET based on resistance selections in cell culture.

9 only occurred in the liver but not in murine cell culture.

10 reduced SARS-CoV-2 entry and infectivity in cell culture.

11 sive profiling of small exRNAs isolated from cell culture.

12 tation operates in phospholipid bilayers and cell culture.

13 and how they influence androgen activity in cell culture.

14 r analysis in any proliferating asynchronous cell culture.

15 ystems is dependent on the complexity of the cell culture.

16 te cancer cells in a PLA device for advanced cell culture.

17 plicates similarly to the wild-type virus in cell culture.

18 1)-Fc fusion protein block VEEV infection in cell culture.

19 d L, can prevent in vitro viral infection of cell cultures.

20 forts aimed at improving Taxol production in cell cultures.

21 red to classical two-dimensional endothelial cell cultures.

22 re also compared on conjunctival and corneal cell cultures.

23 ablation of specific subpopulations in mixed cell cultures.

24 d when ectopically expressed in tobacco BY-2 cell cultures.

25 models of cancers as compared to traditional cell cultures.

26 arance of amyloid beta (Abeta) in microglial cell cultures.

27 sette exchange (RMCE) reactions in mammalian cell cultures.

28 cking Abeta-induced cytotoxicity in neuronal cell cultures.

29 evaluated on primary human nasal epithelial cell cultures.

30 n employed to produce PNIPAm-based films for cell culturing.

31 ated directly from medicinal plants or plant cell culture(1).

32 n+), we found that during acute infection in cell culture, 17dmiR-H1/H6 exhibited a modest increase i

33 despite not compromising fitness in standard cell cultures, a destabilizing H1N1 HA stalk mutation gr

34 wed promising anticancer activity in both 2D cell culture and 3D multicellular tumor spheroid models

35 Using cell culture and animal models, here we sought to identi

36 exhibited low nanomolar antiviral potency in cell culture and encouraging PK properties.

37 and can be used for applications (such as T-cell culture and expansion) requiring high-avidity molec

38 requires basic molecular biology, mammalian cell culture and fluorescence microscopy skills.

39 s have been developed by serial passaging in cell culture and found to be safe in infants.

40 8 gene (vDeltaUL48) is impaired in growth in cell culture and has diminished virulence in vivo In con

41 inhibits invasion, but not proliferation, in cell culture and in a mouse model of tongue cancer.

42 ays and Western blotting during infection in cell culture and in chickens, we determined that the inv

43 7 blocks PDAC tumor growth and metastasis in cell culture and in mice.

44 ect of these mutations on viral infection in cell culture and in vivo is not well understood.

45 of the Kunjin virus helicase on infection in cell culture and in vivo This work provides new insight

46 ts (T407A and S411A Kunjin, respectively) in cell culture and in vivo We were able to recover S411A K

47 paclitaxel (a.k.a. Taxol) that is stable in cell culture and labile to Pd catalysts.

48 Disease modeling using cell culture and mouse models of angiogenesis and wound

49 pressor of colorectal cancer (CRC) growth in cell culture and mouse tumor xenografts.

50 eplacement material to PDMS for microfluidic cell culture and OOC applications.

51 tible light doses and are widely utilized in cell culture and other biological applications.

52 e capable of being propagated in endothelial cell culture and passed to naive cells, indicative of a

53 d reduce cell invasion and migration in both cell culture and three-dimensional spheroids grown from

54 inin peptide-ELPs promising biomaterials for cell culture and tissue engineering.

55 l attachment and has demonstrated utility in cell culture and tissue engineering.

56 yield of 214 mg/l in unoptimized, mammalian cell culture and, in contrast to a stabilized spike ecto

57 In the present study, using mammalian cell cultures and a PD mouse model, along with CD spectr

58 Using primary cell cultures and cell lines, we investigated the involv

59 Functionally these cells initiate stem cell cultures and exhibit mesodermal bias in differentia

60 nduced deficiency of CNN3 in lens epithelial cell cultures and explants results in actin stress fiber

61 osyl-resistant Tau aggregates extracted from cell cultures and human AD brain tissues, demonstrating

62 ins and proper localization of MURC in shPAB cell cultures and in shPAB muscle bundle.

63 een FAM20C and Periostin was investigated in cell cultures and in vitro.

64 pression in vitro in human airway epithelial cell cultures and in vivo in mouse models of ICS adminis

65 n, and antiviral activity are compromised in cell cultures and mice, while autophagic activity is enh

66 lant cells, plant leaf protoplast and fungal cell cultures and observed using confocal microscopy.

67 fluorescence of SWG, we followed its fate in cell cultures and show that its incorporation at the tra

68 etastatic breast cancer cells to invade bone cell cultures and suppresses their ability to form bone

69 as been hampered by low yields of human mast cell cultures and their poor transfection efficiency.

70 ntiation, using two-dimensional human neural cell cultures and three-dimensional forebrain organoid m

71 late) substrate is proposed for in-situ HeLa cell culturing and real-time detection of the released H

72 entiated gemcitabine and JQ1 cytotoxicity in cell culture, and Gem + TSA + JQ1 inhibited tumor initia

73 Using yeast, cell culture, and mouse models of LGMDD1, we found that

74 Escherichia coli, mouse embryonic fibroblast cell cultures, and Arabidopsis thaliana leaves.

75 tro models of VC (i.e., human serum, primary cell cultures, and tissue explants), and largely abolish

76 Flow cytometry, cell culturing, and microscopy may reach these detection

77 increased the activity of testosterone in a cell culture androgen reporter system by competitively d

78 hydrogels films in electronic platforms for cell culture applications.

79 e maintained in the biological conditions of cell culture (aqueous environment, salts, heating), poin

80 Three-dimensional (3D) spheroidal cell cultures are now recognised as better models of can

81 Tick cell cultures are often continuously cultivated, as free

82 3D cell cultures are rapidly emerging as a promising tool t

83 t spontaneous epialleles that arise in plant cell cultures are stably maintained by siRNA and H3K9me2

84 formation in zebrafish embryos and mammalian cell culture, arguing that ciliary defects are at least

85 itro studies in patient-derived organoid and cell cultures as well as in vivo positron emission tomog

86 "dispersed-settled" to distinguish them from cells cultured as a monolayer.

87 nfected 2D neuronal cultures and neural stem cell cultures, as well as in HSV-1-infected 3D neuronal

88 allows for antiviral drug screening in human cell culture at biosafety level 2 (BSL2) with high-throu

89 Experiments with MIN6 cells cultured at different densities provided evidence

90 e-nucleotide polymorphism (SNP) occurring in cells cultured at three collaborating institutes.

91 In human cell culture, bacterial SLs are processed by host SL-met

92 for these MIH neuropeptides in Clytia using cell culture-based "deorphanization" of candidate oocyte

93 The advent of cell culture-based methods for the establishment and exp

94 l cells proliferation and differentiation in cells cultures between either serum preparation.

95 been extensively used for three-dimensional cell culture, bleeding control, and molecular and cell t

96 required for viral replication and spread in cell culture but rather correlated positively with the c

97 cing of unknown viruses without isolation in cell culture, but information on the virus host is often

98 shable from those of wild-type (WT) virus in cell culture, but it displayed smaller plaques and impai

99 new prodrug is effectively uncaged in cancer cell culture by Pd nanosheets captured within agarose an

100 ompared Ifnb induction in mixed murine brain cell cultures by a panel of HSV1 mutants, each devoid of

101 human Mxra8 enables alphavirus infection in cell culture, cattle Mxra8 does not.

102 idly than domestic cat fibroblasts following cell culture challenge.

103 ed with the onset of cavitation bubbles in a cell culture chamber and (2) the dynamics of cavitation

104 ew tool for screening expression profiles of cell culture clones, monitoring purification, and evalua

105 infection and increased cytopathogenicity in cell culture compared to wild-type (WT) Kunjin.

106 quently used to extrapolate from the nominal cell culture concentration to PCB tissue levels and vice

107 the degradation of WGA-labeled GCX under UF cell culture conditions or in Balb/C mice and led to an

108 c with vincristine and LDHA inhibition under cell culture conditions, but this combination was ineffe

109 constitutively active in PEL, but that under cell culture conditions, PEL rapidly became independent

110 . nucleatum viability under standard aerobic cell culture conditions.

111 In human cell culture, deletion of the human HIP orthologs HIP1 a

112 recapitulated the immunogenic properties of cell culture-derived D123-HMW in guinea pigs.

113 ive sulfoglycans from the egg-propagated and cell culture-derived influenza vaccines and demonstrate

114 edia conditions to produce intracellular HEV cell culture-derived particles (HEVcc) with viral titers

115 y to HER2 and integrin alphavbeta6 positive, cell culture-derived, breast and pancreatic cancer-deriv

116 We previously showed that a cell culture-derived, disulfide-linked high-molecular-we

117 During cell culture development, considerable effort is investe

118 tworks are separated by a novel microfluidic cell culture device that allows controlled network-to-ne

119 Matrix gel islands are spotted on a cell culture dish to act as support for receiving and cu

120 n, in two consecutive vintages, and in grape cell cultures elicited with calcium.

121 tion of imaging mass cytometry (IMC) with 3D cell culture enabled single-cell phenotyping at 1 mum sp

122 Primary human amnion epithelial cell cultures established from amnion membranes from non

123 asily performed by an experimenter with stem cell culture experience and take 2-3 months to complete,

124 Their biological evaluation in cell culture experiments and SAR revealed that the compo

125 Cell culture experiments examined the effect of everolim

126 Cell culture experiments finally demonstrated that activ

127 creased claudin-8 expression, confirming the cell culture experiments' findings.

128 ated Wnt/beta-catenin signaling in 2D and 3D cell culture experiments, promoted axis duplication in X

129 By using cell-culture experiments and plasmonic-force calculation

130 ations in human bronchial epithelial BEAS-2B cell cultures exposed to 1 ppm formaldehyde for 2 h.

131 l lines growing in adherent, two-dimensional cell culture express HSATII RNA at different levels, and

132 From electrophysiological recordings in cell cultures expressing Cx43 or Cx45, the principal iso

133 replication in mice, it was not observed in cell culture, ferrets or human challenge participants.

134 and activation by IL-7, IL-23, and IL-12 by cell culture, flow cytometry, and phospho-flow assays.

135 promise as a faster and safer alternative to cell culture for the detection and real-time sequencing

136 k and applied them to exemplar human thyroid cell cultures for 72 h.

137 s are expected to take 8 d, with the primary cells cultured for 12 d to form mature in vitro crypts.

138 /slie) mice, and deletion of Ddr2 in primary cell cultures from dental pulp and PDL inhibited differe

139 ated expression of ACE2 in airway epithelial cell cultures from patients with COPD and in mice with e

140 Choroid plexus cell cultures from rats were used to analyse annexin A5

141 m OC demonstrated multiple fluorescent OC in cell cultures from TRAP tdTomato mice, but not from WT m

142 d metabolism is altered in airway epithelial cells cultured from people with cystic fibrosis.

143 and RvD1 on LTB4-induced responses of goblet cells cultured from rat conjunctiva.

144 fashion, we also profiled the heterogeneous cell cultures generated from multiple human pluripotent

145 Grafts with all cells cultured >=96 hours did not contain more beta cell

146 The subgroup with all cells cultured >=96 hours exhibited longer C-peptide >=0

147 d can be performed directly on the clarified cell culture harvest fluid without the need for Protein

148 Modulating centriole number in 3D cell cultures has been shown to influence protrusive beh

149 ly used to fabricate cell sheet surfaces for cell culturing, however copolymer and interpenetrated po

150 GPgV proliferated in cell culture in a species- and cell-type-specific manner

151 affordable option for end users working with cell culturing in relation to, e.g., diagnostics and dru

152 GBM cells and patient-derived GBM cells cultured in 3D microwells were co-treated with BAY

153 ed and -controlled mechanical impact to live cells cultured in a custom-built in vitro setup compatib

154 ctor 2 (ETV2)(3) in mature human endothelial cells cultured in a serum-free three-dimensional matrix

155 ys were performed for invasive breast cancer cells cultured in mechanically plastic, nanoporous, and

156 Sorted CD34(+) cells cultured in the presence of stem cell factor (SCF)

157 nical mode of invadopodia activity of cancer cells cultured in three-dimensional BM-like matrices.

158 ntal stages were obtained by infecting HCT-8 cells cultured in vitro.

159 k previously trained in mouse embryonic stem cell cultures (InDelphi-mESC) is able to accurately pred

160 el system found that human airway epithelial cell cultures induce a strong proinflammatory cytokine r

161 This frequency was highest if cell culture inoculation occurred on the same day as blo

162 Porous cell culture inserts established a 3-D milieu for bathin

163 In 2-D cell cultures, irradiation with NIR light at low power r

164 Using 3D cell culture, it is shown that drug release is commensur

165 on extensive passaging of orthopoxviruses in cell culture leading to attenuation in vivo, a strategy

166 al life solution application formats such as cell culture media and human serum.

167 e found increased IGF1 protein levels in the cell culture media and increased cellular phosphorylatio

168 indicate that the presence of PR and SLP in cell culture media may significantly impact in vitro stu

169 due to their degradation or sequestration in cell culture media prior to analysis.

170 DAPI released in situ in cell culture media was incorporated into retinal pigment

171 The mean oxygen levels for water and cell culture media were 27.7 +/- 2.1% and 27.6 +/- 4.1%,

172 bution from perfusion media (i.e., blood, or cell culture media) to cells within cell-dense, metaboli

173 or a long-chain fatty acid mixture added to cell culture media, and mass spectrometry-based metabolo

174 detection limits <1 pM in 150 mM buffer and cell culture media, as well as < 10 pM in artificial CSF

175 es and potential mispaired side products, in cell culture media, or other complex matrices.

176 olated by size-exclusion chromatography from cell culture media.

177 perate directly in aqueous solutions such as cells culture media, but they offer much a higher on/off

178 PCBs were present in the cell culture medium (60.7-88.8%), cells (8.0-14.6%), and

179 The nanoparticles were characterized in cell culture medium to assess how their physical propert

180 The osmotic pressure of GBM cell culture medium was adjusted using sodium chloride o

181 In cell culture medium, serum increased the rate of CVB3/28

182 estimate of the PCB levels in the cells and cell culture medium.

183 useful additions to traditional preclinical cell culture methods and in vivo animal studies in the n

184 We generated a cell culture model by CRISPR/Cas9-mediated deletion of C

185 Due to the absence of a robust cell culture model for HEV infection, the analysis of th

186 This study reports the first cardiac cell culture model for MFS, revealing abnormalities in t

187 In conclusion, this robust cell culture model of HEV infection provides a powerful

188 spheroid (MCTS) systems provide an in vitro cell culture model system which mimics many of the compl

189 ablishment of a unique persistently infected cell culture model to study MERS-CoV-bat interactions.

190 Albeit a cell culture model, primary astrocytes may better recapi

191 eta (Abeta40 and Abeta42) in a primary human cell culture model.

192 r nature of the selection scheme employed in cell culture modeling studies can determine which potent

193 regulation during trophoblast development in cell culture models and in vivo.

194 Cell culture models are used to study the toxicity of po

195 Mammalian cell culture models are widely used to study cytotoxicit

196 Three-dimensional (3D) cell culture models mimic the pathophysiological environ

197 Using cell culture models of human OCCC, we find that shRNA si

198 plementary studies using human breast cancer cell culture models revealed that siRNA-mediated silenci

199 ve been hampered by the scarcity of suitable cell culture models that mimic the natural environment o

200 revent alpha-syn aggregation in vitro and in cell culture models with binding affinities of 0.5 muM t

201 Advanced in vitro cell culture models, such as ex vivo culture models or o

202 yV infection has mostly relied on the use of cell culture models, such as SVG-A cells (SVGAs), an imm

203 creased transcription of these same genes in cell culture models.

204 this has mostly been limited to immortalized cell culture models.

205 ined with monitoring secretion efficiency in cell culture of selected N2 single-domain mutants, findi

206 te brain activity using traditional in vitro cell culture on an engineered platform.

207 monstration, the permeability of endothelial cells cultured on hydrogels was electrochemically measur

208 bstantially lower than reported for in vitro cell cultures or for external-beam radiotherapy clinical

209 pproaches for high-level Taxol production in cell cultures or microbial hosts.

210 icating attenuated vaccine candidates or low-cell culture passage clinical isolates from humans or an

211 stable isotope labeling with amino acids in cell culture phosphoproteomic analysis identified Rab7-S

212 ootprint similar to that of a common 24-well cell culture plate within which each well is electrified

213 cell sheets using time-lapse imaging of the cell culture process every 20 minutes over 14 days to as

214 es 8 d to complete and requires expertise in cell culture, protein purification, and chromatography.

215 However, established 3D cell culturing protocols and techniques are time-consumi

216 ause it exhibits sortase-dependent growth in cell culture, providing a robust phenotype for high thro

217 ibility, Fe(0) SI-ATRP can be applied within cell cultures, providing a tool that can broadly and dyn

218 ed stable isotope labeling by amino acids in cell culture (pSILAC)/bioorthogonal noncanonical amino a

219 The cell-cultured quadrivalent vaccine was not significantly

220 ifferent sources (human colorectal carcinoma cell culture, raw bovine milk, and Ascaris suum nematode

221 Results revealed that commonly used cell culture reagents hydrocortisone and cholera toxin s

222 In human MIO-M1 Muller cell cultures, REDD1 deletion prevented oxidative stress

223 eveloped to manage high-dimensional data and cell culture, respectively.

224 Annexin A5 addition to choroid plexus cell cultures restored the Abeta-induced impairments on

225 Extending on the cell culture results, we found that in contrast to WT mi

226 ork presents a detailed investigation on how cell culture's state relates with either model parameter

227 this CD-AOF medium could be used in several cell culture settings.

228 and the survival of various cancer cells in cell culture settings.

229 Pulsed Stable Isotope Labeling in Cell culture (SILAC) approaches allow measurement of pro

230 stable isotope labeling with amino acids in cell culture (SILAC) is a valuable technique to study RC

231 n, stable-isotope labeling by amino acids in cell culture (SILAC)-based proteomics was used to identi

232 eceptor-specific pharmacological analysis in cell culture, simultaneous assessment of dopamine releas

233 tedly mapping active neurons and synapses in cell culture, slice preparations, and in vivo during beh

234 xpressed at high yield in Expi293F mammalian cell cultures, solubilized and purified in Facade deterg

235 assessing the internal microstructure of 3D cell cultures still remains extremely slow because of th

236 Years of in vitro cell culture studies have unraveled myriad macrophage pa

237 However, in vitro cell culture studies may not accurately reflect how macr

238 ing in mice, animal behavioural analysis and cell culture studies to identify a highly conserved regu

239 ers (MCTs), we used a combination of primary cell culture studies, (13)C isotopic tracing, laser micr

240 In cell culture studies, our results demonstrated that the

241 ls maintained the cytotoxic phenotype during cell culture, suggesting a stable phenotype.

242 Virus titers in cell culture supernatants and lung homogenates were dete

243 thod could specifically detect HIV-1 in 293T cell culture supernatants, as well as a set of 11 HIV-1

244 Here we used a cell culture system as proof of concept to show that, up

245 A keratinocyte cell culture system using HaCat cells was established to

246 Using a polarized endocervical cell culture system, we determined that conditioned medi

247 Using a cell culture system, we established that astrocytes rele

248 edict the atropselective partitioning in the cell culture system.

249 st tau pathology in a 3D non-cell autonomous cell culture system.

250 various degrees, depending on the virus and cell culture system.

251 The results from studies conducted in cell culture systems and animals suggest that both vitam

252 demonstrate that click-ExM is applicable in cell culture systems and for tissue imaging.

253 Loss of ZNF410 in adult-type human erythroid cell culture systems and xenotransplantation settings di

254 n of microfabrication, microfluidics, and 3D cell culture systems could be used for the development o

255 Cell culture systems for prion propagation have greatly

256 A wider range of cell culture systems might help overcome these shortcomi

257 The authors used genetic deletion models and cell culture systems to investigate calcium signaling.

258 xpressing cells, largely because the popular cell culture systems utilized for studying HCMV do not e

259 In human T(H) and B-cell culture systems, DNA damage-induced GDR elicited by

260 In contrast to cell culture systems, mouse models are highly favored fo

261 with suspected rickettsioses using mammalian cell culture systems.

262 nd discuss relevant state-of-the-art dynamic cell-culture systems.

263 Models using 3D cell culture techniques are increasingly accepted as the

264 is exacerbated by limitations of traditional cell culture techniques, which fail to recapitulate mech

265 ore, we outline the value of modern in vitro cell culture techniques, with a focus on their current a

266 nging to model PCE using animals and current cell culture techniques.

267 Three-dimensional (3D) cell culture technologies, such as organoids, are physio

268 inal enteroids (HIEs) are primary epithelial cell cultures that can provide insights into the biology

269 largely based on two-dimensional endothelial cell cultures that, due to lack of laminar flow and the

270 Morus alba Diels-Alderase (MaDA), from Morus cell cultures, that catalyses an intermolecular [4+2] cy

271 In a cell culture, the addition of a 3-O-S HS 12-mer reduced

272 In cell cultures, the entire flavonoid pathway was represse

273 In cell cultures, the overexpression of miR-214-3p in endot

274 on methods that fix, destroy, or singularize cell cultures, thereby disrupting each cell's microenvir

275 A(H3N2) seed virus propagated exclusively in cell culture, thus lacking egg adaptive changes.

276 ts within complex biological samples such as cell culture, tissue histology slices, or living subject

277 Competence with human cell culture, tissue processing, immunohistology and mic

278 d stable isotope labelling of amino acids in cell culture to quantify the host proteome response duri

279 c details to perform AQuA-HiChIP, going from cell culture to ranking chromatin interactions within 6

280 an exposure cohorts, a mouse model and human cell culture to test our hypothesis.

281 herpesviruses lacking CHPK can propagate in cell culture to various degrees, depending on the virus

282 vior, and (3) analyzing the response of U2OS cell cultures to a broad array of small molecule perturb

283 a variety of biological systems ranging from cell cultures to animal models.

284 c(Ac)(3) as a novel precursor in large-scale cell cultures to generate usable amounts of O-glycans as

285 ver, the mutation rate is reduced by >50% in cells cultured under 5% oxygen, when we also found alter

286 18 season showed slightly higher RVE for the cell-cultured vaccine compared with SD egg-based vaccine

287 In cell culture, VTE-associated variants of STAB2 had a red

288 ers from a single cell lysate of oral cancer cell cultures was demonstrated.

289 A(H3N2) seed virus propagated exclusively in cell culture, was more effective than egg-derived IIV4 i

290 iquid interface on a stainless-steel grid in cell culture wells containing a nutrient solution.

291 pDCs and T-cell cultures were adoptively transferred before heterot

292 Patient derived xenograft models and cell cultures were successfully created (76% and 44% of

293 itro assays with SH-SY5Y human neuroblastoma cell cultures, where Abeta42 aggregation into large inso

294 efficacy have been conducted on 2D monolayer cell cultures, which are known to be poor models for in

295 metabolically activated and quiescent CD4 T cells (cultured with 2 ng/ml interleukin-7).

296 B3GALT5-KO cells cultured with 2iLAF exhibited naive-like transcrip

297 In vitro, T21 cells cultured with apigenin had significantly reduced o

298 in proximal KIR promoters in CD56(bright) NK cells cultured with ascorbic acid.

299 Mineralization of WT bone marrow stromal cells cultured with conditioned medium from Hdac3-defici

300 e)-On and Myc-Off Tet21N human neuroblastoma cells cultured with U-(13)C(6)-glucose medium.