ライフサイエンスコーパス: cell extract

1 iological samples (e.g., human biofluids and cell extracts).

2 nonfat milk, egg yolk, human serum and HeLa cell extract.

3 has been further tested by analyzing a whole cell extract.

4 ition of viral replication in yeast and in a cell extract.

5 the ribosomal/membrane fraction of bacterial cell extract.

6 vity originating from 380 +/- 20 cancer 293T cell extract.

7 protein substrates in the context of a whole cell extract.

8 SC-related assembly complexes found in human cell extract.

9 ent purification tags directly from HEK-293T cell extract.

10 o isolate the target protein directly from a cell extract.

11 T content of complexes assembled in cells or cell extract.

12 f phosphopeptides from serum digest and HeLa cell extract.

13 ketobenzyl-d4TTPs was proven in T-lymphocyte cell extracts.

14 ins from Thermococcus species 9 degrees N or cell extracts.

15 tners by immunoprecipitation of cross-linked cell extracts.

16 rates of nucleotide excision repair in human cell extracts.

17 NA replication in yeast or in vitro based on cell extracts.

18 in glycoprotein ligand-1) from hematopoietic cell extracts.

19 shown to exist in a common complex in human cell extracts.

20 g partner of full-length CAPERalpha in human cell extracts.

21 ing of R-loops by HeLa and human neuron-like cell extracts.

22 ogram and with labeled standards spiked into cell extracts.

23 glucomutase 1 enzyme activity was assayed on cell extracts.

24 tro and in immunocomplexes purified from the cell extracts.

25 nt clearance of the ErbB-2/HER2 protein from cell extracts.

26 H range 6.1-8.6 with resting cells and crude cell extracts.

27 d reactions in human CD4(+) T-lymphocyte CEM cell extracts.

28 unoprecipitates with gamma-tubulin Gtb1 from cell extracts.

29 cies and were found to be highly abundant in cell extracts.

30 in pure enzyme fractions as well as in crude cell extracts.

31 method for measuring telomerase activity in cell extracts.

32 that lead to a DNA expansion event in human cell extracts.

33 eins encoded by RASopathy genes in mammalian cell extracts.

34 s for normalization of metabolomic data from cell extracts.

35 amount of heme-bound mPGES2 was detected in cell extracts.

36 nute protein amounts readily from tissue and cell extracts.

37 also active against endogenous Tdp1 in whole cell extracts.

38 directly against the purified proteins or in cell extracts.

39 fed a diet supplemented with Wigglesworthia cell extracts.

40 e action of DUBs and the proteasome in crude cell extracts.

41 led based on studies using Xenopus and human cell extracts.

42 substrate delays nucleosome loading in human cell extracts.

43 as specific targets of these probes in whole cell extracts.

44 purified RFC and in associating with RFC in cell extracts.

45 ied in aqueous phosphate buffer and in CEM/0 cell extracts.

46 with Rab38 and Rab32 from MNT-1 melanocytic cell extracts.

47 high resistance toward dephosphorylation in cell extracts.

48 activities were identified from M. smegmatis cell extracts.

49 noprecipitation of (35) S-methionine-labeled cell extracts.

50 ils of how to perform a basic assay of whole-cell extracts.

51 at levels similar to those of PrtP in whole-cell extracts.

52 ursors to prohead oligomers were detected in cell extracts.

53 ified phospholipid species from microfluidic cell extracts.

54 ing activity of Shelterin complexes in human cell extracts.

55 nt to induce WAVE-dependent bead motility in cell extracts.

56 of single spliceosomes in real time in whole-cell extracts.

57 and with purified phosphoproteins from whole cell extracts.

58 from impure solutions or even directly from cell extracts.

59 nscription (RT) PCR using RSV-infected HEp-2 cell extracts.

60 re slowly in dwa1 and dwa2 than in wild-type cell extracts.

61 ique regions within ~3,000 proteins in human cell extracts.

62 mbination with fluorescent derivatization of cell extracts.

63 biquitinated proteins despite high levels in cell extracts.

64 ctivity was assayable in R. prowazekii lysed-cell extracts.

65 on of the illicit drug fentanyl in red blood cell extracts.

66 screening as activators of caspase-3 in HeLa cell extracts.

67 20 muM in the cell-free assay and 61 muM in cell extracts.

68 labeled protein variants in E. coli and HeLa cell extracts.

69 Bax variants were reasonably stable in HeLa cell extracts.

70 own kinases acting on target phosphosites in cell extracts.

71 measuring polymer concentration in cells and cell extracts.

72 aricus exists as a heterotrimeric complex in cell extracts.

73 e G9/A16 EFC subcomplex in detergent-treated cell extracts.

74 nalyzing lipid-protein interactions in crude cell extracts.

75 ry roles in TBSV replication in cells and in cell extracts.

76 nd a decrease in 8-oxoG cleavage activity in cell extracts.

77 upercoils in ICL-containing plasmids in HeLa cell extracts.

78 5F12) to human XPA or in XPC(-/-) fibroblast cell extracts.

79 n of NSP ubiquitylation in Neurospora crassa cell extracts.

80 shared between those formed in cells and in cell extracts.

81 highly selective delivery of the NDP in CEM cell extracts.

82 and Mll4 complexes in size-fractionated beta-cell extracts.

83 tidase activity in proteasomes purified from cell extracts.

84 associate hierarchically when purified from cell extract [1], an observation that is mostly explaine

85 chemical reactivity and metabolism in liver cell extracts, 15 candidate metabolites were identified

86 The MIC values of the probiotic methanol cell extracts against Candida albicans ranged between 1.

87 II, still bound to native DNA, from HEK-293T cell extract, allowing us to calculate a 25-A-resolution

88 nhibition of cleavage and polyadenylation in cell extract and a decrease in the levels of several key

89 ed levels of ubiquitinated proteins in whole-cell extract and at proteasomes, suggesting that UCHL5 a

90 1/dyskerin/NOP10/NHP2 scaffold purified from cell extract and demonstrate that distributed sequence f

91 inding assays were performed using the whole-cell extract and oligonucleotide probes consisting of th

92 Remarkably, cell extract and purified protein experiments show that

93 itro replicase assembly assay based on yeast cell extract and purified recombinant tombusvirus replic

94 the phosphatase activity from a B. subtilis cell extract and suppose that dephosphorylation is catal

95 UreD found to be soluble in Escherichia coli cell extracts and able to complement a DeltaureD-urease

96 P)- and MS-based experiments with mouse beta-cell extracts and compared the results with those from o

97 Our data suggested that HeLa cell extracts and cultured host cells, but not RRL, cont

98 containing single nucleotide bulges in human cell extracts and discovered that several deaminated or

99 nd stoichiometries in protein complexes from cell extracts and from in vitro synthesized components.

100 ay and LC-MS, methylofuran was identified in cell extracts and further purified.

101 tion of E3-selective ubiquitination in whole-cell extracts and high-resolution MS.

102 subsequently purified an activity from HeLa cell extracts and identify this as the E3 ubiquitin liga

103 y to nitrocellulose-blotted human epithelial cell extracts and IgE levels to environmental allergens

104 rescence and stability of the Spinach RNA in cell extracts and in living Escherichia coli cells.

105 ence of deoxyadenosine cleavage in T. brucei cell extracts and increased deoxyadenosine sensitivity i

106 are exclusively (homo- or hetero-) dimers in cell extracts and intact cells.

107 p53, co-immunoprecipitates 133p53alpha from cell extracts and interacts only with p53 molecules that

108 rected probes for profiling kinases in whole cell extracts and live cells.

109 Starting with the cell extracts and passivated slides, the assay requires

110 ctional assay that utilizes active mammalian cell extracts and protein microarrays and identified 1,5

111 In cell extracts and purified form, Endo III is generally m

112 bstrates are repaired to form cccDNA by both cell extracts and purified human proteins.

113 Analysis of soluble cell extracts and purified MCPs by Western blotting show

114 A detailed investigation of cell extracts and purified proteins by top-down MS, NMR

115 fluoro-2'-deoxyuridine was observed, both in cell extracts and spent culture medium (i.e. tumor cell-

116 6) complexes also can be purified from human cell extracts and that Orc6 and Orc1 each contain a sing

117 Although BC is found as a dimer in cell extracts and the carboxylase activities of the two

118 ive D478A FAM20C mutant was detected in both cell extracts and the media.

119 binding protein 1 (TopBP1) form complexes in cell extracts and when expressed from baculovirus vector

120 selectively enrich GFP fusion proteins from cell extracts, and as affinity column ligands for inexpe

121 f in vitro, immunoprecipitated with CaN from cell extracts, and exhibited fluorescence resonance ener

122 ibility with both purified enzymes and crude cell extracts, and exquisite selectivity for NDPs over t

123 d into proteins, we show that in live cells, cell extracts, and in vitro reconstituted complexes, Hex

124 ons on recombinant POP and FAPalpha enzymes, cell extracts, and living cells demonstrated high potenc

125 in complex biological samples, such as human cell extracts, and may consequently find future use in f

126 Fascin and Daam1 coimmunoprecipitated from cell extracts, and silencing of fascin led to a striking

127 , urea-polyacrylamide gel electrophoresis of cell extracts, and staining of cells with 4,6-diamidino-

128 or (AR) and AR-coactivator proteins in LNCaP cell extracts, and TET2 KD increases prostate-specific a

129 rate synthase protein and enzyme activity in cell extracts, and the major citrate synthase (citZ) tra

130 using standard protein mixtures and complex cell extracts, and the method was applied to study the p

131 occur near the Z-DNA-forming region in human cell extracts, and we model these interactions at the su

132 ecifically bind tubulin from clarified crude cell extracts, and, after washing, highly purified tubul

133 were very stable in CD4(+) T-lymphocyte (CEM cell) extracts, and they were substrates for HIV-reverse

134 However, the mechanisms by which B cells extract antigen remain unclear.

135 fore internalization, germinal center (GC) B cells extracted antigen by a distinct pathway using smal

136 Consequently, GC B cells extracted antigen with better affinity discriminat

137 When cell extracts are applied, the surface-tethered antibody

138 y, we show that in the absence of nucleolin, cell extracts are unable to process miR-15a/16 in vitro

139 the effects of polyethylene glycol and yeast cell extract as crowding agents.

140 mino acids using a doubly labeled (15)N(13)C-cell extract as IS.

141 CB1R C-terminus could bind to both CRIP1a in cell extracts as well as purified recombinant CRIP1a.

142 to thiophosphorylate its substrates in yeast cell extracts as well as when produced as recombinant pr

143 ndent mRNA translation in a rabbit reticular cell extract assay (RRL-IVT).

144 enzyme and comparable activity in HeLa whole cell extract assays and potentiate the cytotoxicity of t

145 Cell-extract assays revealed that vinyl chloride reducta

146 In summary, in human cell extracts, BER and NER activities co-exist and excis

147 In cell extracts, binding of AUF1p45 to TTP potentiated TTP

148 EB1, demonstrated to be present in the whole-cell extracts, binds to both the proximal and distal E2-

149 ed during activation of PrtP) was present in cell extracts but was detected neither in the purified a

150 , and mPGES-2 were readily detected in whole cell extracts but were not regulated by IL-1beta.

151 both in vitro and in neuroblastoma (SH-SY5Y) cell extract by affecting the base excision and AP lyase

152 h the HC-HA complex from agarose-overlaid AM cell extracts by an anti-human IalphaI antibody.

153 g degradation into epitopes in primary human cell extracts by mass spectrometry and on epitope presen

154 mmunoprecipitation of CHD1 and Mediator from cell extracts can be ablated by shRNA knockdown of a spe

155 iation of the two endogenous proteins in the cell extract chromatin fraction is considerably increase

156 e study offers a simple representation of a "cell extract" comprising, e.g., a droplet of actomyosin

157 We now show that HeLa cell extracts contain a factor that promotes ATP- and p3

158 compressible contractile gel, representing a cell extract containing a disordered actomyosin network.

159 tion of <1 muU (pmol/min) of PDE activity in cell extracts containing 0.25-10 mug protein.

160 ng glycosidase inhibitors by FluoPol-ABPP on cell extracts containing recombinant, overexpressed glyc

161 ion events in complex environments including cell extracts contribute to our understanding of how the

162 HEK293T cell extracts could add dNTPs to DNA primers hybridized

163 HEK293T cell extracts, deficient in hMLH1, are unable to process

164 Mass spectrometric analyses of whole-cell extracts demonstrated that two proteins encoded by

165 Single molecule imaging of motility in cell extracts demonstrates that the CAP-Gly domain of p1

166 ytometry, and fluorescence quantification of cell-extracted dendrimer-Cy5.

167 ds into a target plasmid then proceeded in a cell extract-dependent reaction.

168 In addition, we show that the cell extracts derived from MacroD-deficient Neurospora c

169 plying two optimized LC methods to bacterial cell extracts detected more than 200 metabolites with le

170 XP-V cell extracts did not add dNTPs to DNA primers hybridize

171 EMSAs with splenic B cell extracts directly demonstrated that YY-1 binds to t

172 ic endonuclease-1 (APE1) and APE1 from human cell extracts efficiently process an rAP site in DNA and

173 el sirtuin deacetylation substrates in whole cell extracts, enabling large-scale screens for new deac

174 The proteomic analysis of the cell extract, Endoplasmic Reticulum/Golgi apparatus and

175 by chemical hydrolysis and in particular by cell extract enzymes.

176 nsor of Abl tyrosine kinase activity in HeLa cell extracts, exhibiting low luminescence with extracts

177 We further validated the assay using whole cell extracts, extending its potential application to de

178 separation process of DNA or protein from a cell extract extremely important.

179 The MCM7 or SF3B3 depleted cell extract failed to splice reporter RNA in in vitro R

180 PX2 and demonstrate the utility of mammalian cell extracts for biophysical assays.

181 Fas signaling, we screened primary lymphoma cell extracts for Fas-associated proteins that would hav

182 easured and compared in identical human HeLa cell extracts for the first time under identical conditi

183 ever, been hampered by its rapid reversal in cell extracts, for example through the action of de-ubiq

184 In contrast, (31)P NMR of whole-cell extract from capsule-knockout strains (Deltacps) cl

185 Here, using cell extracts from APTX-deficient cell lines, human Atax

186 ortantly, the activity of TACE was higher in cell extracts from CERK(-/-) as compared with wild type.

187 In acute inflammatory cell extracts from exercised mice, we found elevated exp

188 We found that protein-free cell extracts from H. salinarum provided a high level of

189 Using cell extracts from HeLa cells, as well as transfection o

190 different mutations appears similar in whole-cell extracts from lymphocytes, and suggest that measure

191 with the KSHV K-bZIP protein in vitro and in cell extracts from lytically reactivated infected cells.

192 Incubation of the L-selectin tail with cell extracts from phorbol 12-myristate 13-acetate-stimu

193 Cells extracted from aortic tissue were analyzed and cat

194 U and found that the thresholds of V1 simple cells extracted from in vivo recordings in awake behavin

195 ern of peripheral chromatin in the nuclei of cells extracted from liver and thyroid specimens is asso

196 Gene expression analyses of leukemia cells extracted from the BM identified Cn-dependent sign

197 em cells is present in CD146(+) perivascular cells extracted from the nonhematopoietic adipose tissue

198 eatures (descriptors) often used to quantify cells extracted from these images have long been shown u

199 Bone marrow and splenic cells extracted from tumor-bearing and control mice (n=

200 of >98% in pre-purified mesenchymal stromal cells, extracted from human dental pulp, with no adverse

201 ition, we observed identical productivity of cell extracts generated from culture volumes spanning th

202 , or in vitro methylation assays using whole cell extracts grown under different conditions.

203 ressed protein translation in cell-free HeLa cell extract; however, it did not affect translation in

204 Mass spectrometric analysis of cell extracts identified 352 proteins that could be matc

205 bits transcription of 5S rRNA genes in whole cell extracts, if this precedes the stage of initiation

206 support replication of plasmid DNA in yeast cell extracts in a reaction that exhibits hallmarks of c

207 specific Box D complex formed with infected cell extracts in electrophoretic mobility shift assay ex

208 litates PARP-1 association with XPA in whole cell extracts, in isolated chromatin complexes, and in v

209 ion of the hydrophilic fraction of the whole-cell extracts increased the antifungal activity of the h

210 Assays of DNA repair in intact cells or in cell extracts indicate that this protein damage compromi

211 Co-immunoprecipitation of the protein from cell extracts indicated that RNase II interacts with its

212 more, the transplantation of iMuSCs or their cell extracts into the muscles of mdx mice (i.e., a mous

213 e primary siRNA-binding protein in the whole cell extracts is Dicer.

214 hexametaphosphate added to X. autotrophicus cell extracts is metabolized to trimetaphosphates, suppo

215 showed that mRNA 3' end cleavage reaction in cell extracts is strongly but transiently inhibited unde

216 e found a defect in both activities in human cell extracts lacking LIG4.

217 A end joining, and addition of wt-Metnase to cell extracts lacking Metnase markedly stimulated DNA en

218 Cell extracts lacking Metnase poorly supported DNA end j

219 Examination of cell extracts led us to detect a previously unknown xant

220 riggered mechanism in human T-lymphocyte CEM cell extracts loosing first the AB moiety, followed by t

221 performed by polymerase chain reaction or by cell extract-mediated recombination.

222 y(A)(+) RNA interactome capture with a whole-cell extract normalization procedure.

223 g was quantified in culture supernatants and cell extracts of HuH-7 cells transiently transfected wit

224 rtate was the only source of beta-alanine in cell extracts of M. jannaschii.

225 y overcome by methylating DNA in vitro using cell extracts of M. xanthus prior to transformation.

226 determination of human active vitamin B12 in cell extracts of Propionibacterium freudenreichii subsp.

227 or acrylate to propionyl-CoA was detected in cell extracts of R. sphaeroides grown with 3-hydroxyprop

228 Compared with cell extracts of radiation-sensitive bacteria, H. salina

229 tric analyses and activity assays with whole cell extracts of strain CBDB1 gave strong evidence that

230 In cell extracts of the M. maripaludis wild-type strain, th

231 The NER efficiencies in human HeLa cell extracts of these lesions are significantly differe

232 his issue, we have studied NER in human HeLa cell extracts of two topologically distinct lesions, one

233 compatible and was proven to be inert in in-cell extracts of Xenopus laevis oocytes at 18 degrees C

234 eptional characterization T4 PNK activity in cell extracts, offering a powerful tool for biomedical r

235 us molecules that could be detected in whole-cell extracts, OMV preparations, and lipopolysaccharide

236 model was further validated using N. tabacum cell extracts or recombinant N. tabacum protein prenyltr

237 neuronal and human embryonic kidney (HEK293) cell extracts overexpressing PAG/Cbp, we show that EphB2

238 NEIL2 immunocomplexes from cell extracts preferentially repaired the mutagenic cyto

239 ddition of purified recombinant GAPDH to the cell extract prepared from GAPDH-depleted yeast results

240 ith a high-molecular weight complex in whole cell extracts prepared from two different cell lines.

241 ifugation to enrich for mitochondrion within cell extracts prior to DNA extraction, short-read sequen

242 obtained between 2- and 12-fold dilutions of cell extracts (R(2) = 0.999).

243 In cells and cell extracts, raising cGMP also enhanced within minutes

244 vitro; depletion of endogenous RtcB in HeLa cell extracts reduces U6/L1 RNA ligation efficiency; ret

245 AD9 knocked-down human prostate cancer whole cell extracts, relative to controls.

246 gen conversion into androgens in strain DHT3 cell extracts requires methylcobalamin and is inhibited

247 The analysis of cell extracts revealed that the heme of GlbN was covalen

248 In cells and cell extracts, RUTBC1 influenced the ability of Rab32 to

249 We used a novel whole-cell-extract screening approach to identify Spt7, a memb

250 of purified PARP-1, and in experiments with cell extracts, self-PARylation was greater in Hmgn1(+/+)

251 inant cytoplasmic exonuclease Xrn1 and liver cell extracts show that miR-122-mediated protection of t

252 x, with immunoprecipitation experiments from cell extracts showing association of hsc70 with wild typ

253 sensor to quantify CDK4 activity in melanoma cell extracts, skin biopsies and melanoma xenografts.

254 Moreover, adding ubiquitinated proteins to cell extracts stimulated proteasome binding of both enzy

255 bonucleoprotein-free state of tRNA halves in cell extract suggest that ciliate tRNA halves are degrad

256 increased level of Erv46-cargo complexes in cell extracts suggesting that disulfide linkages in the

257 t, restores ALY recognition in IPMK-depleted cell extracts, suggesting a mechanism underlying transcr

258 oU proteins were partially activated by HeLa cell extracts, suggesting that a host cell cofactor othe

259 ying RNA-dependent PABP-eIF4G association in cell extracts suggests that RNA1, the PABP-binding domai

260 d Sit4 physically interact with eIF2alpha in cell extracts, supporting their direct roles as eIF2alph

261 Crude cell extracts synthesize ITA via decarboxylation of cis-

262 ght into somatic cells, we developed a human cell extract system that recapitulates CDK1 activation a

263 Because cell extract systems only approximate in vivo conditions

264 We show by using cell extracts that Ku is essential for the efficient rem

265 However, when utilizing HeLa whole cell extracts, the difference in incision of THF-in vers

266 this reaction has been detected in mammalian cell extracts, the protein(s) responsible remain unknown

267 ous activity of both alpha-galactosidases in cell extracts, thereby providing a means to study the pr

268 tating active RP534 from R. prowazekii lysed cell extracts, thus verifying that this protein is expre

269 ced in a PDZ-regulated manner by addition of cell extract to near in vivo levels.

270 ct) that utilizes easy to generate bacterial cell extracts to assemble multiple DNA fragments into re

271 IA-ESI-MS).We used series dilutions of HL-60 cell extracts to establish the relationship between cell

272 eins, Kel1 and Kel2, associate with Bud14 in cell extracts to form a stable 520-kDa complex with an a

273 ere we used affinity chromatography of tumor cell extracts to identify LGALS3BP as a novel sialic aci

274 tion and reduces the capacity of Arabidopsis cell extracts to ligate a nicked DNA intermediate.

275 id adenine dinucleotide phosphate (NAADP) in cell extracts using surface-enhanced Raman spectroscopy

276 RNAs spliced from biotin-tagged pre-mRNAs in cell extracts, using antibodies to EJC components Y14 an

277 al complement-evasion molecules, C. albicans cell extract was absorbed to a factor H-coupled matrix.

278 n vitro assembly of the viral replicase in a cell extract was inhibited by the cytosolic fraction obt

279 ion in the endogenous pol beta gene, and the cell extract was subjected to an 'affinity-capture' proc

280 ion experiments (IPs) carried out with whole-cell extracts (WCEs) revealed that hemagglutinin (HA)-ta

281 sing affinity purifications from C. albicans cell extracts, we demonstrate that CaTAF12L uniquely ass

282 sembly on membrane-coated beads in mammalian cell extracts, we found that Rac1 was not sufficient to

283 environmentally regulated FLO11 promoter in cell extracts, we identified 15 regulators that bound sp

284 In cell extracts, we identified a novel activity that speci

285 lectrophoretic mobility shift assays in HeLa cell extracts, we show that OA treatment disrupts pre-mi

286 o note that the measured quantities from the cell extract were found to constitute a statistically si

287 Cell extracts were analyzed for mutant protein stability

288 rmal NER activity appeared when the XPC(-/-) cell extracts were complemented with XPC-RAD23B proteins

289 treated with 100 microg/ml IgG for 6 h, and cell extracts were examined by immunoblot using Abs to p

290 entrations in supernatants of culture and in cell extracts were measured using Illumina multiplex Eli

291 Whole-cell extracts were obtained from cultured human corneal

292 with both polysomal and monosomal RNA in S2 cell extracts, whereas Y14 and MAGO fractionate separate

293 ision repair (NER) experiments in human HeLa cell extracts which show that the G*CT* intrastrand cros

294 ited PCNA-dependent DNA synthesis in a human cell extract with improved avidity when compared with th

295 eptides (AQUA peptides) spiked in HeLa human cell extract with limits of quantification of 17.2 amol

296 found that Def1 copurifies from yeast whole-cell extract with TFIIH, the largest general transcripti

297 Immunoprecipitation of muscle cell extracts with a PUM2 specific antibody precipitated

298 yeast cells, as primase does not interact in cell extracts with pol1 that either terminates at residu

299 agged protein can be specifically labeled in cell extracts without protein purification and then can

300 plexes with single Sp or Gh lesions in human cell extracts yields a characteristic nucleotide excisio