ライフサイエンスコーパス: cell lysate

1 s following stimulation with an EBV-infected cell lysate.

2 hieved for the detection of ERalpha in MCF-7 cell lysate.

3 is advantageous to measure miRNA in a crude cell lysate.

4 ve standard deviations of up to 70% in crude cell lysate.

5 nd dimers were successfully pulled down from cell lysate.

6 cterize full-length otoferlin from mammalian cell lysate.

7 espite the presence of growth media and Vero cell lysate.

8 h the protein(s) they react with in cells or cell lysate.

9 ed MALAT1 ENE+A RNA upon addition of HEK293T cell lysate.

10 mutant virus protein in the transfected BHK cell lysate.

11 strated with standard proteins and a complex cell lysate.

12 rks for complex mixtures of trypsin-digested cell lysate.

13 om a complex sample such as blood, serum, or cell lysate.

14 ectrometer for three proteins and an E. coli cell lysate.

15 he dominant AEBSA target in diluted serum or cell lysate.

16 at high concentrations as well as of a crude cell lysate.

17 of the kinase activity and inhibition in the cell lysate.

18 ntingtin aggregation in a cell model and its cell lysate.

19 e complex and slow with both whole cells and cell lysate.

20 ous concentrations in prostate cancer (PC-3) cell lysate.

21 complex biological samples such as serum and cell lysate.

22 the extraction of plasmid DNA from bacterial cell lysate.

23 these ligands, even in samples as complex as cell lysate.

24 ies of pyrophosphopeptide in the presence of cell lysate.

25 ormation from nanogram quantities of protein cell lysate.

26 es were protective compared to an uninfected cell lysate.

27 with standard protein mixtures and a complex cell lysate.

28 ross-linked protein-DNA complexes from yeast cell lysate.

29 with a concomitant retention of sGAG in the cell lysate.

30 buffered saline (PBS) and spiked in E. coli cell lysate.

31 put quantification of unlabeled compounds in cell lysate.

32 20,000 phosphopeptides from 100 mug of MCF-7 cell lysate.

33 st effectively used with purified protein or cell lysate.

34 wt % of the total protein from breast cancer cell lysates.

35 t of detection 0.3ng/mL (1.5pM) for ERBB2 in cell lysates.

36 able to profile molecular activities within cell lysates.

37 tivity of ITPA in bacterial, yeast and human cell lysates.

38 of overexpressed proteins directly in crude cell lysates.

39 (CANC) nanotubes captured SUN1 and SUN2 from cell lysates.

40 molecules and unpurified protein targets in cell lysates.

41 ied proteins, protein complexes or clarified cell lysates.

42 relevant concentrations of ERBB2/neu/Her2 in cell lysates.

43 known endogenous protein binders in complex cell lysates.

44 eins added in crude Escherichia coli or 293T cell lysates.

45 tion of SPSB2-iNOS interaction in macrophage cell lysates.

46 7 bacteriophage and His6-GroEL directly from cell lysates.

47 t of hTS with one such peptidic inhibitor in cell lysates.

48 in the lysosomal fraction, but not in whole-cell lysates.

49 e for two different grades of bladder cancer cell lysates.

50 es individual caspases can cleave in complex cell lysates.

51 es, to complex aggregates, as found in total cell lysates.

52 vity changes in hundreds of cysteines within cell lysates.

53 ipitated with IGF1 receptor (IGFR1) in whole-cell lysates.

54 mains that are unstructured when analyzed in cell lysates.

55 of NAAAR mutant gene libraries directly from cell lysates.

56 w separation of as little as 20 mug of total cell lysates.

57 little as 25 mug tryptic peptides from whole cell lysates.

58 extraction of phosphopeptides from the whole cell lysates.

59 PLC fractionation of Chlamydia-infected HeLa cell lysates.

60 ures and for protein-protein interactions in cell lysates.

61 single-molecule motility assays of mammalian cell lysates.

62 -fructofuranosidase activity in T. vaginalis cell lysates.

63 and can be recapitulated by eosinophil whole-cell lysates.

64 molecules and unpurified protein targets in cell lysates.

65 rove their binding to endogenous PDEdelta in cell lysates.

66 er to enrich for such methylated lysine from cell lysates.

67 NAD(+)-dependent desuccinylation activity in cell lysates.

68 n the culture media, but increased it in the cell lysates.

69 apture the endogenous protein from mammalian cell lysates.

70 s interacted with the P. falciparum-infected cell lysates.

71 ence coverage when applied to S. pombe whole cell lysates.

72 unpurified esterases in bacterial and human cell lysates.

73 ge using either protein standards or complex cell lysates.

74 as well as profiling glycosylase activity in cell lysates.

75 with either FLAG or DYKD epitopes from crude cell lysates.

76 proteins that bind to rRNA G-quadruplexes in cell lysates.

77 ole amounts of ADP-ribosylated peptides from cell lysates.

78 he activity of caspase-7 in Escherichia coli cell lysates.

79 njugated and thioesterified forms of Ub from cell lysates.

80 n (out of 35 fractions) from a crude HEK 293 cell lysate, a total of 640 proteins were identified in

81 In CHO-IR cell lysates, a glutathione S-transferase chimera of the

82 is capable of detecting miRNA-143 in cancer cell lysates, allowing for the discrimination between th

83 rcial reagent that can easily generate crude cell lysates amenable to direct analysis by one-step RT-

84 on and increased ENaC abundance in the total cell lysate and at the cell surface.

85 Through cell lysate and cell assays, a promising lead candidate

86 filing of alpha-mannosidases from both human cell lysate and mouse tissue extracts.

87 mination of proteins from minimal amounts of cell lysate and opens more opportunities for the isolati

88 in vitro translation assay containing human cell lysate and purified target mRNA fused to a reporter

89 SOD, GR, and CAT activities in red blood cell lysate and saliva and MDA levels in plasma and sali

90 ose concentration was measured for mammalian cell lysate and serum, which led to estimates of low-mM

91 Ub-tetrazole probe was also assessed in HeLa cell lysate and showed robust labeling only upon photoac

92 single-molecule pull-down (SiMPull) from HEK cell lysate and subunit counting in the plasma membrane

93 d secosterol A and B (Seco A and Seco B), in cell lysates and apical washes.

94 of this iBody for the isolation of FAP from cell lysates and blood serum as well as for its detectio

95 teomic comparison between metaphase-arrested cell lysates and chromosome-sorted samples reveals a coh

96 l, we tested varicella zoster virus-infected cell lysates and clinically isolated virus and found evi

97 We also found that HpGroES bound to TLR4 in cell lysates and colocalized with TLR4 on the cell membr

98 RNA target mir-21 could be detected in crude cell lysates and detected by imaging in live cells.

99 AX-haptenated proteins were detected in cell lysates and extracellularly, either as soluble prot

100 nation of the presence of SLT-1 from complex cell lysates and ham/juice samples based on the detectio

101 ctin glycoprotein ligand-1 (PSGL-1), both in cell lysates and in cell-free assays.

102 nuclear antigen expression, respectively, in cell lysates and individual cells.

103 reduced the rate of c-di-GMP degradation in cell lysates and inhibited the activity of EAL-dependent

104 ee AAV system, we purified AAVs from HEK293T cell lysates and medium by polyethylene glycol precipita

105 kdown also increased phospho-VEGFR2 in whole cell lysates and membrane fractions compared with contro

106 arative assessment of all Htt transcripts in cell lysates and mouse tissues without the need to first

107 xpressed equivalent amounts of HgbA in whole-cell lysates and outer membranes.

108 imultaneous detection of two miRNAs in crude cell lysates and proved that the method was robust when

109 rapid and sensitive detection of miRNAs from cell lysates and serum samples.

110 Gag peptides were detectable in both cell lysates and supernatants in CD4+ T cells infected i

111 Virus from cell lysates and synthetic templates could be readily am

112 can successfully pull-down peptide-MHCs from cell lysates and the aAPCs generated using this techniqu

113 ines demonstrated that LH2 is present in the cell lysates and the conditioned media in a dimeric, act

114 nd coproporphyrin, based on HPLC analysis of cell lysates and the culture medium, as well as cell-fre

115 n complex physiological environments such as cell lysates and to measure their individual activities

116 ed that CacyBP/SIP forms a homodimer in NB2a cell lysate, and biophysical methods demonstrated that C

117 sis of immunoprecipitation product from OSCC cell lysate, and in situ proximity ligation assays.

118 midimidoyl fluoride electrophile, with human cell lysate, and the protein conjugates formed were iden

119 s and phosphopeptides in clinical specimens, cell lysates, and mouse liver tissue samples, demonstrat

120 ng, and ELISA, we analyzed expression media, cell lysates, and purified proteins for FXII activation.

121 exceeds 10 pM while miRNA concentrations in cell lysates are below 1 pM.

122 Single-cell lysates are generated at defined time points and an

123 eptide assay-based kinome analysis (in which cell lysates are used to phosphorylate specific kinase s

124 also identifies native CAII in human kidney cell lysate as an AEBSA target.

125 tion of NF-kappaB by C. trachomatis-infected cell lysates as a biomarker for the presence of PG fragm

126 lex, is broad in scope, and is applicable in cell lysates as well as to covalent inhibition/modulatio

127 nce-based quantification of this fraction in cell lysate at a time point of interest.

128 Reproducibility for metabolites in cell lysate averaged 9% RSD.

129 Levels of copper were measured in cell lysates, blood samples, liver homogenates, and subc

130 ESD in HEK293T cells, we detected WT MESD in cell lysate but not in conditioned medium, whereas the c

131 to no ability to suppress dicer activity in cell lysates, but higher expression of B2, following hea

132 med to interrogate itaconate's reactivity in cell lysates, but methods for analyzing targets of itaco

133 port on accurate miRNA quantitation in crude cell lysate by a CE-based hybridization assay termed dir

134 fication of intracellular FA release through cell lysate calibration.

135 We also show that MDA-MB-231 cell lysates can "seed" aggregation of the central core

136 ermore, the alkyne-tagged glycoproteins from cell lysates can be directly detected with AzBOCEt in ge

137 , Kd values for unpurified proteins in crude cell lysates can be obtained without prior knowledge of

138 n vitro DISC is assembled in the presence of cell lysate, caspase-8 Y380 phosphorylation attenuates D

139 ied a high quantity of mRNA from crude yeast cell lysate compared to a phenol/chloroform extraction m

140 of soluble, assembled RuBisCO recovered from cell lysates compared with co-expression of RuBisCO with

141 pectroscopy and elemental Zn in bio-chelated cell lysate complex was confirmed by SEM and Energy Disp

142 Addition of cell lysates containing baculovirus-expressed B2 to lysa

143 that pretreatment of cell free extracts and cell lysates containing Sav mutants with diamide affords

144 The cell lysate content was measured with scanning probe mic

145 pH-exposure of DM-DO in cell lysates corroborates such a pH-regulated mechanism,

146 Protein captured from a crude bacterial cell lysate could also be deuterated without the need fo

147 ltiple kinase activity profiling from single cell lysate could potentially allow us to study heteroge

148 We previously demonstrated that crude cell lysates could be used for some limited downstream D

149 ly analyze the genome of SARS-CoV-2 in human cell lysate, demonstrating the capability and the utilit

150 biotin-labeled viral dsRNA or poly(I.C) and cell lysate-derived or in vitro translated G3BP1 indicat

151 fibronectin by peritoneal cell-derived mast cell lysates diminished GBS adherence.

152 rol the transport and handling of each 17-pL cell lysate during analysis.

153 On-chip cell lysate electrical impedance spectroscopy has been u

154 ntities of SGPs could be detected from total cell lysates, even at a signal-to-noise ratio of as low

155 polyacrylamide gel electrophoresis of single cell lysates followed by an in-gel immunoassay.

156 cent isotope abundance of (15)N-ammonia in a cell lysate for (15)N-isotope tracing studies.

157 Following cell lysis and sampling crude cell lysate for analysis, the substrate and the product

158 he dimensionality of datasets is shown using cell lysates for all possible combinations of up to 9 fi

159 ion of O-glycans from glycoproteins in whole cell lysates for mass spectrometric analysis.

160 hrome c (Cyt c) is an important biomarker in cell lysates for the early stage of apoptosis or antican

161 yl di- and tripeptides in Chlamydia-infected cell lysate fractions.

162 Furthermore, in vitro assays with crude cell lysate from PCE grown cells revealed dechlorination

163 is of GLD is based on GALC activity of total cell lysates from blood, which does not discriminate whe

164 f differentially expressed proteins in whole cell lysates from E. coli.

165 l inflammation, ii) the capacity of necrotic cell lysates from HT29 cells or human IECs to induce hum

166 analysis (HILIC-RPLC) of S. cerevisiae whole cell lysate has been used to acquire retention informati

167 From whole tissues to single-cell lysate, heterogeneous immunoassays are widely utili

168 s; and the presence of chlorotyrosine in the cell lysates, hypochlorous acid is hypothesized to be th

169 -top-down MS analysis of Pyrococcus furiosus cell lysate identified 134 proteins and 291 proteoforms

170 still detected in detergent-treated infected cell lysates.IMPORTANCE The entry of enveloped viruses i

171 act proteoforms from 5 mug of intact E. coli cell lysate in 10 online-collected fractions.

172 Single-cell lysate in each microwell is "electrophoretically pr

173 -1 protein is 0.0078mg/ml of T24 (Grade III) cell lysate in phosphate buffered saline, artificial uri

174 in our platform by both tracking fluorescent cell lysate in sealed microwells and with a human-mouse

175 strong cation exchange-fractionated HEK293T cell lysate in XL-MS experiments using disuccinimidyl su

176 lated compound 73 enriched NS4B protein from cell lysates in pull-down studies, and the findings toge

177 report a simple platform for trapping single-cell lysates in sealed, picoliter microwells capable of

178 lows reproducible near-complete digestion of cells lysates in 1-5 min.

179 r peptide-MHCs can directly be captured from cell lysates, including cancer cells using affinity bead

180 multiple Dhb-modified proteins in mammalian cell lysates, including histone H3, a previously unknown

181 ns identified in a tryptic digest of E. coli cell lysate increased by 13% and 14%, respectively, alon

182 e receptor ligands and M. tuberculosis whole-cell lysate, increased M. tuberculosis replication, and

183 th Milli-Q(TM) water and the infiltration of cell lysate into the porous matrix was monitored by meas

184 Less than 1 muL of cell lysate is required as starting material to solve th

185 rine A with cyclophilin A protein in a yeast cell lysate is successfully detected and quantified usin

186 Dicer activity in crude cell lysates is increased in the presence of Ca(2+), mos

187 and RIG-I coimmunoprecipitation of infected cell lysates isolated immunostimulatory CCHFV RNA.

188 in each microwell; (iii) PAGE of each single-cell lysate; (iv) exposure of the gel to UV light to blo

189 oints toward the potential of oxidized tumor cell lysate-loaded SNAs as a potent class of immunothera

190 rease in CEST image contrast was observed in cell lysates (mean +/- standard deviation, 0.52% +/- 0.0

191 consisted of invasively collecting samples (cells, lysates, media, etc.) from an OOC.

192 atalytically significant cytochrome P450s in cell lysate, microsomes, and bacteria.

193 The bisacetate is more effective in cell lysates, more cytotoxic in prostate cancer cells th

194 Chemical treatments of M. tuberculosis whole-cell lysates (MtbWL) ruled out protein, nucleic acid, an

195 Western blot analysis of total cell lysate obtained from normal human mammary epithelia

196 for detection of solubilized p16 protein in cell lysates obtained from patients.

197 tudy, a microparticulate vaccine using whole cell lysate of a murine ovarian cancer cell line, ID8 wa

198 When used on data obtained from the cell lysate of an unexplored oncogenic cell line, it rev

199 m to rapidly identify SLT-1 from the complex cell lysate of E. coli O157:H7.

200 accompanying HNSCC biomarkers from a single cell lysate of oral cancer cell cultures was demonstrate

201 components of PRC2, NuRD, and SIN3A from the cell lysate of the TNBC cell line SUM149.

202 Analysis of whole cell lysates of mouse brain, liver, red muscle fibers, a

203 Both whole cells and cell lysates of Oxf strains HC1 and OxWR were analyzed u

204 ng AcP activity in both enzyme solutions and cell lysates of prostate cells.

205 ncrease in CEST-MRI contrast was obtained in cell lysates of rdLRP transfected cells and in in vivo L

206 Cell lysates of the cyanobacterial mutants further funct

207 e responsible for degrading pGpG, we assayed cell lysates of WT and orn strains of P. aeruginosa PA14

208 lysis of endogenous alpha-syn from red blood cells lysate of healthy controls and PD patients.

209 tes peptide samples from protein mixtures of cell lysate or body fluid origin.

210 d upon incubation with human lens-epithelial cell lysate or lens homogenate.

211 Cell lysate or mitochondria were isolated using standard

212 However, selectivity was restored by crude cell lysate or purified G-actin, which joined PPP1R15-PP

213 d TSLP receptor-knockout mice with sham HeLa cell lysate or RV.

214 s (in the form of a defined peptide antigen, cell lysates or antigens sourced from AML cells recruite

215 on and transformation results collected from cell lysates or fixed-cells conceal important dynamic in

216 to study samples of limited lifetime such as cell lysates or whole cells.

217 it acts as a biochemical precursor to NAD(+) Cell lysates possess an ATP-dependent kinase activity th

218 Necrotic cell lysates potently induced HIF IL-6 and IL-8 producti

219 Y4137 significantly increased cAMP levels in cell lysates prepared from inner medullary collecting du

220 a linear dependence on the concentration of cell lysate present while specificity is demonstrated by

221 er cell types, nc886 bound to PKR in human T cell lysates, preventing PKR phosphorylation by polyinos

222 d band broadening, even when handling single-cell lysate protein concentrations in an open device.

223 ss spectrometric analysis indicated that 628 cell-lysate proteins enriched in 44 cellular component c

224 Cell-lysate proteins regulated by TGF-beta1 were charact

225 eport here that HBc expressed in a mammalian cell lysate, rabbit reticulocyte lysate (RRL), was able

226 ting CYP119 mutants containing Ir(Me)-PIX in cell lysates, rather than as purified enzymes.

227 RNase treatment of cell lysates reduces Gzm cleavage of RNA binding protein

228 ion of substance P, periostin, and red blood cell lysate (representing hemosiderin).

229 ide protein interactions in E. coli and HeLa cell lysates, respectively, identifying 1,158 and 3,301

230 tein kinases and 15 lipid kinases from human cell lysates, respectively.

231 recipitation of viral proteins from infected cell lysates revealed association of UL84, UL44, and nuc

232 were determined for miRNA samples in a crude cell lysate, RNA extract from the lysate, and a pure buf

233 in targets were discovered from a eukaryotic cell lysate (Saccharomyces cerevisiae).

234 teins from 9 injections from a single Jurkat cell lysate sample consisting of 400 ng of total protein

235 try (LC/MS) quantifications of irinotecan in cell lysate samples were used to compare the results fro

236 pplied to detect pathogenic Leptospira whole cell lysates samples with the satisfactory results.

237 the quantitation of ErbB2 in human serum and cell lysates samples without any matrix effect.

238 visualization of BfrB-iron in P. aeruginosa cell lysates separated on native PAGE gels and stained f

239 Assays of cell lysates showed that ATP-dependent 5-oxoprolinase ac

240 Moreover, the addition of purified RelE to cell lysates shows promise as a method for generating ri

241 of effectively enriching SLT-1B from complex cell lysates simply by pipetting 20 muL of the sample in

242 l samples was assessed by testing samples of cell lysate solutions obtained from human astrocytoma (g

243 binding domains (LBDs) can be recruited from cell lysates specifically onto their target phospholipid

244 r the extraction of DNA from crude bacterial cell lysate spiked with 1 pg mL(-1) template DNA without

245 din-Sepharose beads to GL-biot-treated DU145 cell lysates, STAT3 was isolated and identified as a tar

246 nt complexity were compared: (1) data from a cell lysate study performed in our lab, (2) data from an

247 ABPP on crude cell lysates suggested that general proteome thiol react

248 ce and human patients compared with lymphoma cell lysates, suggesting a concentration of these factor

249 e specific activity of M. tuberculosis whole-cell lysates, suggesting that a polysaccharide was requi

250 -dependent desuccinylation activity in crude cell lysate systems, as compared to the desuccinylation

251 t of affinity-modulating adaptor proteins in cell lysates that would be absent in ligand screening or

252 crowded cellular environment and eukaryotic cell lysates, that parameters optimized toward a pseudo

253 on with an immunoassay-based analysis of the cell lysate, the platform allowed the selective and sens

254 ollowing PIL-based SPME of DNA from a dilute cell lysate, the qPCR amplification efficiency was deter

255 omal protein could be obtained directly from cell lysates, thus leading to an improved method to reco

256 NPB) for sensitive and selective analysis in cell lysates, tissues, and serums by mass spectrometry (

257 onality) to a tryptic digest of whole Jurkat cell lysate to estimate the depth of proteome coverage a

258 thase, was purified in only ten minutes from cell lysate to near homogeneity (>90 %).

259 Six proteins were purified from complex cell lysates to average homogeneities of 76 %.

260 ible to purify phytase simply by heating the cell lysate, to drive aggregation of non-cyclized protei

261 g a multistep enrichment strategy from whole cell lysate, to evaluate their abilities to enrich for d

262 RT-PCR of cell lysates transfected with rdLRP demonstrated a stabl

263 dividual SOS molecules are captured from raw cell lysate using Ras-functionalized supported membrane

264 ) can be specifically pulled from K562 human cell lysates using beads decorated with phosphorylated g

265 s can be assessed by fractionating tissue or cell lysates using differential ultracentrifugation thro

266 nhibition by small molecules and activity in cell lysates using parallel DNA sequencing or quantitati

267 ions (1 x 10(2) CFU/ml) was detected in FCDI cell lysates using real-time PCR with greater consistenc

268 nd quantitation of carbonyl metabolites from cell lysate was accomplished using a carbonyl-reactive f

269 S analysis (SCX-RPLC) of S. cerevisiae whole cell lysate was used to generate a retention dataset of

270 When a B16F10 melanoma cell lysate was used to load DCs with tumor antigens dur

271 ity to enrich members of the CDK family from cell lysates, was investigated.

272 the labels for Rictor and mTOR in the whole cell lysate (WCL) blots were swapped in Fig.

273 resis to grossly enrich GSK-3beta from whole cell lysate, we discover by MRM-MS a novel O-GlcNAcylate

274 In the analysis of MCF7 cell lysate, we show collision-induced dissociation (CID

275 -induced conversion assays with RML-infected cell lysates, we observed a strong signal over all six p

276 Cell lysates were prepared and reacted with glutathione-

277 Further, Zn enriched cell lysates were prepared by Ultrasonication, as an org

278 MiR-214 levels in exosomes but not in cell lysates were reduced by pretreatment of the cells w

279 ed affinity probes report on KAT activity in cell lysates, where KATs exist as multiprotein complexes

280 ons and in complex environments of mammalian cell lysates, where relative amounts of free reactive ox

281 These assays are performed on cell lysates, which circumvents the labor-intensive step

282 o the quantitative determination of Cyt c in cell lysates, which opens a new avenue to early diagnost

283 fied a subset of HDAC8 substrates from human cell lysates, which were further validated for catalytic

284 ation of BSH activity with purified protein, cell lysates, whole cells, and in human gut microbiome s

285 Separation of a cell lysate with a 60 min gradient showed extremely high

286 ferase added in the Escherichia coli or 293T cell lysate with better staining sensitivity than conven

287 and purification of DNA from crude bacterial cell lysate with subsequent quantification by real-time

288 thylated proteins are enriched by incubating cell lysates with 3xMBT, or with the binding-null D355N

289 rated using proteome-wide reactions of HT-29 cell lysates with a model probe of threonine beta-lacton

290 Moreover, incubations of cell lysates with deuterated (2E)-hexadecenal revealed t

291 mmunoprecipitation assay of mouse ameloblast cell lysates with either ameloblastin or Psma3 antibody

292 formation can be performed in blood serum or cell lysates with minimal interference from biomolecules

293 sed the phosphorylation status of Mga in GAS cell lysates with Phos-tag gels.

294 large number of MTs (>50) from human cancer cell lysates with remarkable specificity over other clas

295 ecipitation of rat liver or transfected HeLa cell lysates with rOATP1A1 antibody specifically co-immu

296 attomoles of glycoproteins of interest from cell lysates, with sensitivity several orders of magnitu

297 5-HT concentrations in the culture media and cell lysates without changing the expression of 5-HT syn

298 of a low-abundance transcription factor from cell lysates without need for isolation or enrichment.

299 ll plates, and also allows the direct use of cell lysates without the need for DNA purification or nu

300 of antibodies against LcrV, Y. pestis whole-cell lysate (YPL), and F1 antigen and more balanced IgG1