ライフサイエンスコーパス: cell separation

1 delay at the terminal stage of cytokinesis (cell separation).

2 lating neural progenitor cell (NPC) daughter cell separation.

3 ign and operation of size-based microfluidic cell separation.

4 plications such as regenerative medicine and cell separation.

5 ther with synthases, for growth and daughter cell separation.

6 of cleavage furrow ingression and the end of cell separation.

7 enzymes called "amidases" to drive daughter-cell separation.

8 cruit EnvC to the septum but fail to promote cell separation.

9 c kinase that acts as a spatial inhibitor of cell separation.

10 cytoplasmic compartmentalization and (viii) cell separation.

11 ced by the divisome must be split to promote cell separation.

12 sion of the outer membrane (OM) and daughter cell separation.

13 e four genes produces only a modest delay in cell separation.

14 he cleavage of connective septal PG to allow cell separation.

15 trafficking of cargo molecules required for cell separation.

16 of LytF that is necessary and sufficient for cell separation.

17 lysis of septal peptidoglycan, which enables cell separation.

18 equired for septal PG splitting and daughter cell separation.

19 -regulated genes involved in mother-daughter cell separation.

20 s that function in cell wall remodelling and cell separation.

21 of cells consistent with a role for AmiA in cell separation.

22 murein amidases, AmiD did not participate in cell separation.

23 with the major peptidoglycan amidases during cell separation.

24 t caps is induced in correlation with border cell separation.

25 cytokinesis, including septum formation and cell separation.

26 ter cell-specific transcription required for cell separation.

27 cts and disassembles, resulting in delays in cell separation.

28 of this model to study the mechanics of cell-cell separation.

29 resolve membrane connections to bring about cell separation.

30 gions that act in concert to ensure complete cell separation.

31 low outer membrane constriction and daughter cell separation.

32 tereotypical manner, which leads to daughter cell separation.

33 to the target cell, thus allowing effective cell separation.

34 e abnormal thick septa leading to defects in cell separation.

35 utants, mid2delta mutants had delays in cell-cell separation.

36 Skp1 homologue and the F-box protein Pof6 in cell separation.

37 rm the cleavage furrow and complete daughter cell separation.

38 ntly to support transcription and facilitate cell separation.

39 ibility to the L domain and subsequent virus-cell separation.

40 lar bud site selection in diploid cells, and cell separation.

41 EN gene MOB1 is required for cytokinesis and cell separation.

42 her and daughter cells after cytokinesis and cell separation.

43 used additional constrictions and a delay in cell separation.

44 the bud neck in late anaphase, just prior to cell separation.

45 ing pathways to regulate cell morphology and cell separation.

46 ce, spindle formation, nuclear division, and cell separation.

47 f1p splits into two rings, disappearing upon cell separation.

48 and septum synthesis and disappears prior to cell separation.

49 It took as little as 5 min to achieve cell separation.

50 sion and also a defect in cytokinesis and/or cell separation.

51 TGFbeta2 antibody inhibited endothelial cell-cell separation.

52 le revealed that Pob1p is also essential for cell separation.

53 fect is in cytokinesis, septum formation, or cell separation.

54 the cell surface and inhibition of daughter cell separation.

55 f secretion to the bud neck is necessary for cell separation.

56 luding cell expansion, organ initiation, and cell separation.

57 esions with enhanced migration and transient cell separation.

58 ightly controlled to execute proper daughter cell separation.

59 roper execution of cytokinesis and efficient cell separation.

60 is utilized to achieve the DEP-based on-chip cell separation.

61 ed in the floral abscission zone just before cell separation.

62 ntify the impact of the micromagnets on rare cell separation.

63 pathways to regulate septum formation and/or cell separation.

64 tin rings on mother and daughter cells after cell separation.

65 rphase through to the contractile ring until cell separation.

66 plication space in the field of microfluidic cell separation.

67 nd vesicle trafficking in the later steps of cell separation.

68 ed in cell cycle control and mother-daughter cell separation.

69 by target enzymes called amidases to promote cell separation.

70 in, suggesting they are limiting factors for cell separation.

71 n assembly required for septum synthesis and cell separation.

72 al forces of the cell turgor pressure during cell separation.

73 nctions of alpha(1-3)glucan in septation and cell separation.

74 luidic channel was developed for high-purity cell separations.

75 aling occurs for those cells at smaller cell-cell separations.

76 gene receptor cells (U87EGFRvIII) at varying cell separations.

77 rmed multicellular clumps through incomplete cell separation, 10 increased invertase expression, none

78 ings appear to be involved primarily in cell-cell separation, a late stage in cytokinesis.

79 nt skin disorder characterized by suprabasal cell separation (acantholysis) of the epidermis.

80 Upon cell separation after cytokinesis, the new cell ends ado

81 dprA mutant overexpresses ComM, which delays cell separation after division.

82 Conventional cell separation against multiple markers generally requi

83 l wall hydrolases (amidases) responsible for cell separation (AmiA, AmiB and AmiC).

84 -acetylmuramyl-l-alanine amidase involved in cell separation (AmiC), as compared with three largely r

85 y, and we provided the first evidence that a cell separation amidase can utilize a small synthetic PG

86 results thus suggest that the order in which cell separation amidases and their activators localize t

87 C), as compared with three largely redundant cell separation amidases found in Escherichia coli (AmiA

88 her than the zinc cofactor typically used by cell separation amidases, potentially protecting its abi

89 r to further analyze the process of root cap cell separation and a root cap specific promoter for tar

90 we show that the cells were viable after the cell separation and a sample from one spot could be tran

91 shortly after it is made to promote daughter cell separation and allow outer membrane constriction to

92 ssion by LexA-Sin3 and also cause defects in cell separation and altered colony morphology.

93 N. gonorrhoeae results in severely impaired cell separation and altered peptidoglycan (PG) fragment

94 herin is retained on the cell surface during cell separation and augmenting cell-cell adhesion does n

95 l-length AtlA protein did not restore normal cell separation and biofilm formation.

96 l-wall processing during the growth and cell-cell separation and designated the gene as cell-division

97 Plant cell separation and expansion require pectin degradation

98 f using microelectronic chip arrays for both cell separation and gene expression profiling provides a

99 ing root tissues by inducing auxin-dependent cell separation and hydraulic changes in adjacent cells.

100 Five others cause frequent cell lysis during cell separation and map to two loci.

101 Cell separation and mixing experiments demonstrated that

102 Disruption of the spm1+ gene interferes with cell separation and morphogenesis.

103 ual motile cells in which genes for daughter cell separation and motility are ON, and chains of sessi

104 remains locked in a high SlrR state in which cell separation and motility genes are OFF for extended

105 tic switch for controlling genes involved in cell separation and motility.

106 ins binds to and represses genes involved in cell separation and motility.

107 induction by auxin is required to coordinate cell separation and organ emergence.

108 tial, this activity is critical for daughter cell separation and outer membrane invagination during d

109 cell wall constituents that are critical for cell separation and pathogenesis.

110 hat increased AmiC activity also resulted in cell separation and PG fragment release defects, indicat

111 evealed parameters for how AmiC functions in cell separation and PG fragment release.

112 activity but is only partially required for cell separation and polarized growth.

113 hat flagella-based forces initiated daughter cell separation and provided a source for membrane tensi

114 ce of the flow-through centrifuge applied to cell separation and resuspension and to DNA purification

115 sorter, we demonstrate simultaneous on-chip cell separation and sizing with three different samples

116 his patterned thermoresponsive films enables cell separation and sorting by modulating temperature- a

117 activity alone is insufficient for daughter cell separation and that lytic transglycosylases RlpA an

118 led that Glu-229 is critical for both normal cell separation and the release of PG fragments by gonoc

119 ded cells, migrated to the bud neck prior to cell separation and then rapidly relocalized to the inci

120 r phenotypes observed were lysis, failure of cell separation and/or cytokinesis, impaired bud growth

121 n increases sigmaD-directed gene expression, cell separation, and autolysis.

122 Both ace2Delta and ace2-35 show defects in cell separation, and both can rescue the growth defects

123 logue Cbk1, which controls polarized growth, cell separation, and cell integrity.

124 ime- dependent destruction of actin bundles, cell separation, and cell loss.

125 tip, relocates to the mother-bud neck before cell separation, and finally migrates to the incipient b

126 CD34(+) cells were isolated by magnetic cell separation, and high-density oligonucleotide microa

127 ty of the cell wall, disrupts cell division, cell separation, and impairs the fitness of the human pa

128 ork are required for cellular morphogenesis, cell separation, and maintenance of cell integrity.

129 of settings such as thrombosis, immunology, cell separations, and diagnostics.

130 There are increasing number of cell separation applications where cells needs to be sep

131 While cell purity was >95% using both cell separation approaches, gene expression analysis rev

132 hanisms of apple cortex textural changes and cell separation are discussed.

133 nd that the periplasmic amidases that aid in cell separation are minor players, cleaving only one-six

134 ive in DNA segregation and the completion of cell separation, are motile and still fail to localize D

135 n and chain formation, a hallmark of progeny cell separation arrest.

136 uggest that YneA is able to inhibit daughter cell separation as well as septum formation.

137 on events associated with the completion of cell separation as well as the gain of motility.

138 Inactivation of sceD resulted in impaired cell separation, as shown by light microscopy, and "clum

139 er describes central events of budding yeast cell separation, as well as the control pathways that in

140 In vitro cell separation assays show that structural proteins, pe

141 motility and decreased dynamics of transient cell separations associated with cleft formation; inner

142 the mutant cells became apparent by impaired cell separation at the end of cell division and by resis

143 Our data suggest the great potential of cell separation based on conductivity-induced DEP using

144 with the use of techniques for magnetic bead cell separation based on expression of these 3 markers.

145 Cell separation based on microfluidic affinity chromatog

146 tal analysis by magnetophoresis and magnetic cell separation based upon differences in intracellular

147 (AtlA) was recently shown to be involved in cell separation, biofilm formation, and autolysis.

148 lta cells reveals that Ace2p is required for cell separation but not for polarized growth.

149 T) is required not only for constriction and cell separation, but also for initiation of peptidoglyca

150 aughter-specific gene products contribute to cell separation by degrading the cell wall.

151 serial thin-section electron microscopy, and cell separation by protoplasting.

152 (L) budding domains mediate efficient virus-cell separation by recruiting host ESCRT and ESCRT-assoc

153 oteins with LytM domains also participate in cell separation by stimulating amidase activity.

154 ynthesis or remodeling, which in turn affect cell separation, cell envelope integrity, and vibrioid m

155 the analytical operations needed: red blood cell separation, conditioning, enzymatic recognition, an

156 lished that ethylene and auxin contribute to cell separation control.

157 , the normal mechanisms of yeast budding and cell separation create permanent scars which expose suff

158 o1p deficiency in yeast (myo1Delta) causes a cell separation defect characterized by the formation of

159 This latter phenotype leads to a cell separation defect in ndc10-1 cells.

160 or the essential pof6 gene display a similar cell separation defect noted in skp1 mutants, and Pof6 l

161 lay disorganized, diffuse septin rings and a cell separation defect similar to septin deletion strain

162 In addition, we observe an enhanced cell-separation defect in a myo1spa2 strain at 37 degree

163 more, the phosphomimic mutation can suppress cell separation defects of hgc1.

164 escue the lysis but not the cell polarity or cell separation defects of ramDelta cells.

165 inactivation of Mcs6 in csk1(+) cells causes cell separation defects or growth arrest, respectively,

166 ble mutant showed no growth abnormalities or cell separation defects, suggesting that these enzymes a

167 deleted or overexpressed have septation and cell separation defects.

168 emonstrate the efficiency of marker-specific cell separation, DEP-activated cell sorting (DACS) was a

169 rmodynamics-derived approach we analyzed the cell-separation dependence of the signaling stability, a

170 ular, cell-cell signaling can depend on cell-cell separation distance and can influence cellular arra

171 ese trajectories to identify a range of cell-cell separation distances where the signaling was most s

172 enerates a special and unique side-explosive cell separation due to an instantaneous primary septum t

173 m-positive cell wall at the site of daughter cell separation during cell division.

174 ibutes to cell wall disassembly occurring in cell separation during fruit abscission, but its role, i

175 ggests a new mechanism for the regulation of cell separation during the M/G(1) phase transition.

176 found to play crucial roles in P. aeruginosa cell separation, envelope integrity and antibiotic resis

177 l organs abscise after pollination, and this cell separation event is controlled by the peptide INFLO

178 ase is an important but poorly characterized cell separation event.

179 degradation of pectins is required for many cell separation events in plants, but the role of pectin

180 Cell separation experiments suggest that IL-10 can effec

181 lts will enable design of practical particle/cell separation, filtration, and focusing systems for cr

182 ndent pathway that is required for efficient cell separation following cytokinesis.

183 by electron microscopy indicated a defect in cell separation following mitosis.

184 ring constriction, septation, and subsequent cell separation following ring disassembly.

185 nt passive marker in label-free particle and cell separation for chemical, biomedical, and environmen

186 cribe a modified protocol for immunomagnetic cell separation for efficient isolation of human periphe

187 This leads to an optimal initial cell-cell separation for fastest receptor-ligand binding, whi

188 In the realm of cell separation for screening, there has been significan

189 umber of attributes that can be utilized for cell separation, for example, cell shape, cytoskeletal p

190 evaluate the efficacy of immunomagnetic rare cell separation from non-Newtonian particulate blood flo

191 om an endothelial cell surface layer and red cell separation from the endothelial cell surface were m

192 aromyces cerevisiae prevents mother-daughter cell separation, generating multicellular 'snowflake' ye

193 Importantly, application of cell separation, genome-wide expression, and cell-specif

194 re is a sense that the field of microfluidic cell separation has achieved a high level of maturity ov

195 Magnetic cell separation has become a popular technique to enrich

196 hnological work associated with microfluidic cell separation has been driven by needs in clinical dia

197 Immunomagnetic cell separation has been shown to be a highly attractive

198 The reported cell separation has the potential to impact the forensic

199 morphogenetic abnormalities and a defect in cell separation; however, remarkably, cytokinesis appear

200 mpartments) occurring 18 min before daughter cell separation in a 135-min cell cycle so the two const

201 mising applications of the DEP separator for cell separation in a continuous mode.

202 g furrow ingression, membrane resolution and cell separation in budding yeast.

203 Though initially designed for cell separation in DNA sequencing protocols, the flow-th

204 reakage in Kanzi apples and, in contrast, by cell separation in Golden Delicious apples.

205 his allows high-throughput dielectrophoretic cell separation in high conductivity, physiological-like

206 tions; however, only RlpA can support normal cell separation in low-salt media.

207 Among the factors promoting cell separation in mutant cells was a protein of previou

208 resent a simple approach for high-throughput cell separation in parallel in nanoliter droplets and de

209 , both spatially and temporally, with border cell separation in pea root caps.

210 w O(2) inhibited root development and border cell separation in pea seedlings.

211 Thus, the ultrafast daughter cell separation in S. aureus appears to be driven by acc

212 t than the wild-type cells (50% higher), the cell separation in the mutant is slower and often incomp

213 hree dimensions, resulting, after incomplete cell separation, in the 'bunch of grapes' cluster organi

214 ge cross section, together with differential cell separation, indicate the presence of europium parti

215 Impeded cell separation is accompanied by abnormalities in myosi

216 or multinucleate phenotype, indicating that cell separation is also inhibited.

217 For lymphocytes, cell separation is best achieved by fluorescence activat

218 Swift and efficient cell separation is difficult and a major hurdle for isot

219 Cell separation is mediated by autolysins, whose genes a

220 r results therefore support a model in which cell separation is stimulated by the reversible relief o

221 One important aspect of magnetic cell separation is the degree to which a cell binds para

222 Endosperm cell separation is therefore thought to play a critical

223 ct chitin synthase that acts during or after cell separation, is transported normally in chs6 mutants

224 anges in ethylene sensitivity, including the cell separation layer throughout tomato flower abscissio

225 ession of this murein hydrolase activity and cell separation levels to those of the wild-type strain.

226 In this work, we report a scaled, label-free cell separation mechanism called non-equilibrium inertia

227 We previously reported a cell separation method that uses the fluorescence-activa

228 The study presents a dielectrophoretic cell separation method via three-dimensional (3D) nonuni

229 The cell separation method we employed has, as its final ste

230 s an efficient, high-throughput microfluidic cell separation method.

231 We present a dielectrophoresis (DEP)-based cell-separation method, using 3D electrodes on a low-cos

232 Current affinity-based protein or cell separation methods use binary 'bind-elute' separati

233 In model 2, a cell separation model, the mRNA levels of these inflamma

234 genes overlapped significantly with those of cell separation mutants sep10 and sep15.

235 Currently, cell separation occurs almost exclusively by density gra

236 ng 4 (VPS4) follows CHMP4B to this site, and cell separation occurs immediately.

237 RP is crucial to ensure that auxin-regulated cell separation occurs solely along their shared walls.

238 proteins with specific functions to support cell separation of vegetative bacilli and growth in infe

239 od for extraction of NAD(P)(H) from cultured cells, separation of analytes by capillary electrophores

240 tance of considering the potential effect of cell separation on gene expression as well as DNA methyl

241 Following cell separation on the microdevice, DNA extraction, ampl

242 equired for motility but not at the level of cell separation or flagellum biosynthesis.

243 It can be scaled up for routine laboratory cell separation or implemented on a miniaturized scale.

244 During filamentous growth, no cell separation or subsequent beta-glucan exposure occur

245 Cell separation, or abscission, is a highly specialized

246 lls into known auxin-regulated LRP-overlying cell separation pathways, and speculate how PDLP5 may fu

247 centus are highly pinched at multiple sites (cell separation phenotype) and they do not divide to pro

248 ackground and causes slow-growth and delayed-cell-separation phenotypes in the S288C strain backgroun

249 onally designed microfluidic magnetophoretic cell separation platform capable of throughputs of 240 m

250 In this microfluidic cell separation platform, flexible cells with high defor

251 In cell separations, positive selection (capture of the tar

252 within analytical chemistry and proteomics, cell separations predominantly rely upon the second, lab

253 ) was applied to several clinically relevant cell separation problems, including the purging of human

254 oteins was found to be released while border cell separation proceeds.

255 IKE2 (HSL2), have been shown to activate the cell separation process that leads to organ abscission.

256 be selectively digested during the critical cell separation process.

257 ntional laboratory methods, showing that the cell separation product in the outlet reservoir was of m

258 he mother-bud neck, and septum formation and cell separation rapidly ensue.

259 cyte growth factor (HGF) induces endothelial cell separation, regulates expression of cell adhesion m

260 involved in pheromone response and daughter cell separation, respectively.

261 e transcriptase polymerase chain reaction of cell separations showed that the increased production of

262 The demonstrated cell separation shows promising applications of the DEP

263 nd hypothesize that CST negatively regulates cell separation signaling directly and indirectly.

264 We conclude that elements of the same cell separation signaling module have been adapted to fu

265 the orchestration of events during the final cell separation step of cell division called abscission.

266 region of Gag that is critical for the virus-cell separation step) is involved in controlling particl

267 In addition, cell separation studies and flow cytometric analyses rev

268 Cell separation studies showed that bright gp70(+) cells

269 Cell separation studies showed that the increased argina

270 Cell separation studies showed that the unresponsiveness

271 tunately, many current selection methods for cell separation, such as magnetic activated cell sorting

272 odeoxynucleotides prevented endothelial cell-cell separation, suggesting that Slug acts early in the

273 m leukapheresis product with a GMP-compliant cell separation system and placed in 5-day (short) or 21

274 haped electrode design can be applied to any cell separation system, regardless of the separation met

275 ortant role in such a sedimentation-involved cell separation system.

276 oretic field-flow-fractionation (DEP-FFF), a cell-separation technique that exploits the differences

277 Cell separation techniques for droplets have been report

278 Utilizing cell separation techniques, it was determined that the w

279 Physical cell separation techniques, such as cell sorting and las

280 For analysis of rare cell separation, the local viscosity distribution is cal

281 Although Gef3p is not essential for cell separation, the simultaneous disruption of gef3(+)

282 , including two genes required for efficient cell separation: the chitinase-encoding gene CTS1 and th

283 cytokinesis, and also functions to maintain cell separation through much of the subsequent interphas

284 a wide range of applications including blood cell separation, ultrasound contrast agent preparation,

285 This quadruple mutant is delayed at cell separation upon release from mitotic arrest.

286 )-cross-linked pectic polymers and extensive cell separation upon tissue disruption.

287 Cell separation using dielectrophoresis as well as elect

288 iched preparations were isolated by magnetic cell separation using the FDC-restricted monoclonal anti

289 controls transcription of genes involved in cell separation, we show that disruption of some of thes

290 ces between individual cell types, efficient cell separations were achieved by dielectrophoresis on t

291 Cell separations were achieved in a thin chamber equippe

292 RNA-binding protein Scw1 and severely delays cell separation when combined either with a septin mutat

293 ease of fabrication and use is suitable for cell separations when subsequent analysis of target cell

294 TGFbeta2 mediates initial endothelial cell-cell separation while TGFbeta3 is required for the cell

295 art1 cells lyse during cell separation with a thinner and defective septum.

296 Thus, we propose that Cdc14 coordinates cell separation with mitotic exit via FEAR-initiated pho

297 circulating tumor cells, and for label-free cell separation with potential applications in biologica

298 Organ detachment requires cell separation within abscission zones (AZs).

299 tion of Neisseria gonorrhoeae ltgC inhibited cell separation without affecting peptidoglycan monomer

300 me atmospheric composition stimulated border cell separation without significantly influencing root g