ライフサイエンスコーパス: cell stage

1 ess into the blastocoel at the 6th cycle (62-cell stage).

2 wly formed B cells until the ultimate plasma cell stage.

3 e Caenorhabditis elegans embryo up to the 16-cell stage.

4 rease in 5-methylcytosine (5mC) at the eight-cell stage.

5 rs in the bone marrow beginning at the pro-B cell stage.

6 ferentiation is arrested at the guard mother cell stage.

7 gly is not asymmetrically localized at the 4-cell stage.

8 urther developed to the B220(+)CD19(+) pro-B-cell stage.

9 cell divisions, starting at the blastula 64-cell stage.

10 NA and sgRNAs into Nile tilapia embryos at 1 cell stage.

11 ween organisms, but is usually after the two-cell stage.

12 ls were arrested at the B220(+)CD19(-) pro-B-cell stage.

13 rlier in human embryos at the four- to eight-cell stage.

14 t becomes depleted in the posterior by the 4-cell stage.

15 ker protein, arrest development at the pre-B-cell stage.

16 ells were first found at the double-positive cell stage.

17 t fail to progress beyond the transitional B cell stage.

18 mally upregulated TET3 was detected at the 4-cell stage.

19 ce of TET3 was high only at the zygote and 2-cell stage.

20 mocyte maturation up to and beyond the pro-T-cell stage.

21 ne segment-cleavage events at the immature B cell stage.

22 at the common lymphoid progenitor to preproB cell stage.

23 ent that in most cases was arrested at the 2-cell stage.

24 ment, and subsequent transition to the pre-B cell stage.

25 pre-B cells and decreases at the immature B cell stage.

26 two-cell stage to ~90 nJ . s(-1) at the 1024-cell stage.

27 L chain loci become accessible at the pre-B cell stage.

28 at the common lymphoid progenitor to preproB cell stage.

29 the common lymphoid progenitor (CLP) LY6D(+) cell stage.

30 l (mature) B cell value by the cycling pre-B cell stage.

31 ocus in zebrafish after injection at the one-cell stage.

32 nd the germ line are determined by the eight-cell stage.

33 in a severe developmental block at the pre-B cell stage.

34 d/or in the maintenance of a stem/progenitor cell stage.

35 (KO/KO) mutant embryos died before the 32-64-cell stage.

36 in peripheral B cell development at the T1 B cell stage.

37 es, with rapidly declining levels at the two-cell stage.

38 ng an inversion of division order at the two-cell stage.

39 ting memory B-cell populations to the plasma cell stage.

40 as they transition from the RTE to the MN T cell stage.

41 performed ablation experiments at the eight-cell stage.

42 lumen or multiple-lumen phenotype at the two-cell stage.

43 blocks B cell specification at the prepro-B cell stage.

44 early embryonic lethality prior to the eight-cell stage.

45 cell-fate switch occurred at the pIIa-pIIb 2-cell stage.

46 e have found that Notch is active from the 4-cell stage.

47 d domains (DMDs) of imprinted genes at the 8-cell stage.

48 rotein levels are heterogeneous at the eight-cell stage.

49 cells buried inside the embryo around the 16-cell stage.

50 y during embryonic development before the 16-cell stage.

51 and that Padi6-/- embryos arrest at the two-cell stage.

52 es secondary rearrangement at the immature B-cell stage.

53 -) embryos failed to survive after the eight-cell stage.

54 2 is required for development beyond the two-cell stage.

55 f a block in B cell development at the pre-B cell stage.

56 embryos arrested development at the 2- to 5-cell stage.

57 kappa gene recombination at the early pro-B cell stage.

58 its the accessibility of the zygote at the 1-cell stage.

59 ctions converge in both chromosomes by the 8-cell stage.

60 and the nucleolus also appeared around the 8-cell stage.

61 ogically associating domains (TADs) at the 1-cell stage.

62 ing zygotic genome activation (ZGA) at the 2-cell stage.

63 opment in the bone marrow at the small pre-B cell stage.

64 support embryonic development beyond the two-cell stage.

65 nificantly reduced from the 2-cell to the 48-cell stage.

66 mbryo occurs in two distinct phases at the 8-cell stage.

67 ve splice form bias hierarchy, regardless of cell stage.

68 tmentalization can be found as late as the 8-cell stage.

69 h surprisingly continued to divide to the 16-cell stage.

70 established with a drastic increase at the 8-cell stage.

71 Oct4 contributes to the DHSs gained at the 8-cell stage.

72 ulthood by vegfaa mRNA injections at the one-cell stage.

73 the earliest into the most mature erythroid cell stages.

74 profile compared with lymphomas from other B-cell stages.

75 cell development between the pro-B and pre-B cell stages.

76 nd aPKC and cell compaction at the 8- and 16-cell stages.

77 oliferation at the CD34(+) and CD34(-) pro-B cell stages.

78 by conditionally deleting Mcl-1 at various T cell stages.

79 romosomes, respectively, in the zygote and 2-cell stages.

80 e embryo developing to the 32-, 64-, and 128-cell stages.

81 SI was also studied for embryos at different cell stages (1-, 2-, 4-, 8-, and 16-cell stage) to inves

82 ased (1) ROS levels at the 1-, 2-, 4-, and 8-cell stages; (2) H3K9me3 levels at the 2-, 4-, and 8-cel

83 omeres from Xenopus laevis embryos at the 50-cell stage (~200 ng yolk free protein/blastomere) genera

84 Cs) sporadically express preimplantation two-cell-stage (2C) transcripts, including MERVL endogenous

85 Only 53% and 8% of mature eggs reached the 2-cell stage after IVF in animals receiving a 3 and 5 days

86 injected embryos with azidosugars at the one-cell stage, allowed the zebrafish to develop, and detect

87 bp2 results in a complete block at the pro-B cell stage and a lack of mature B cells.

88 arrested differentiation at the large pre-B-cell stage and caused derepression of cell adhesion and

89 MBGs became apparent at the 4-cell stage and contributed to the initiation of EGA, whe

90 I-I proteins are detected as early as at two-cell stage and exhibit distinct and dynamic expression p

91 tosis for nutrient acquisition at its single-cell stage and for antibacterial defense at its multicel

92 anscription factor GATA3 is induced at the 4-cell stage and is consistently present during pre-implan

93 hereas PBGs preferentially appeared at the 8-cell stage and might affect embryo compaction and trophe

94 In spite of a severe block at the pro-B cell stage and profound B cell lymphopenia, significant

95 that are expressed after the KIT+ progenitor cell stage and remain expressed through CD19+ and AICDA+

96 g, host HSCs arrested at the short-term stem cell stage and remained in the marrow in a quiescent cel

97 ks early B cell development at the pre-pro-B cell stage and renders B cell progenitors unresponsive t

98 differentiation events as early as the eight-cell stage and soon thereafter for proper division of th

99 genome activation and DHS formation at the 2-cell stage and that Oct4 contributes to the DHSs gained

100 ocysts and TET1 protein was present at the 4-cell stage and the blastocysts.

101 hat cell polarity is established by the four-cell stage and then reiteratively lost during subsequent

102 polarisation of blastomeres at the 8- and 16-cell stage and then the maintenance of trophectoderm lin

103 or three cycles after the 5th cell cycle (32-cell stage) and ingress into the blastocoel at the 6th c

104 utoreactive cells at the emergent immature B cell stage, and a relaxed selection for peripheral toler

105 activated in mouse embryos as early as the 4-cell stage, and becomes spatially restricted by late bla

106 was detectable at differing levels by the 64 cell stage, and IP(3)-induced Ca(2+) transients could be

107 e mouse embryo undergoes compaction at the 8-cell stage, and its transition to 16 cells generates pol

108 es, spent significantly longer time in the 2-cell stage, and started expressing Plag1 ectopically fro

109 r Aurkb nor Aurkc is expressed after the one-cell stage, and that AURKC is more stable during maturat

110 BAFFR is already expressed at the immature B cell stage, and that the prosurvival protein Bcl-2 does

111 hese data further support the development of cell-, stage-, and/or receptor-specific anti-TNF-alpha t

112 vantage beginning at the late transitional B-cell stage; and (3) a similar in vivo selective advantag

113 ges; (2) H3K9me3 levels at the 2-, 4-, and 8-cell stage; and (3) the expression of IRE-1 and CHOP at

114 ydrogenase, and aconitase, resulted in a one-cell stage arrest before entry into mitosis: pronuclear

115 ation mutation (DeltaN-Zfp36l2) leads to two-cell stage arrest of embryos derived from the homozygous

116 -1 remained inhibitory-phosphorylated in one-cell stage-arrested embryos, indicative of a G2-like arr

117 es pointing to the peripheral transitional B-cell stage as a major juncture where transient autocrine

118 d between the T2 and the mature follicular B cell stage as a result of a partial defect in BCR signal

119 B cell differentiation to the CD19(+) pro-B cell stage as well as survival of CD19(+) pro-B cells.

120 In C. elegans the 4-cell stage blastomere EMS is an endomesodermal precursor

121 al when confined to half of the embryo via 2-cell stage blastomere injections, the latter does not pr

122 The 8-cell stage blastomere MS is primarily a mesodermal precu

123 ibe how to establish EPSCs from single eight-cell-stage blastomeres or whole eight-cell pre-implantat

124 stent with a random distribution up to the 8-cell stage but not at later stages.

125 ivity are mostly deleted before the mature B cell stage, but are positively selected and expanded in

126 ial deletion at the transitional to mature B cell stage, but become Env(-) upon receptor editing.

127 itis elegans embryo is elaborated at the one-cell stage by the polarization of the partitioning (PAR)

128 genes are established at the embryonic stem cell stage by two parallel, but distinct, repressor path

129 s repressed at the pro-B cell and immature B cell stages by the kinase Akt through its 'antagonism' o

130 The two-cell stage Caenorhabditis elegans embryo divides asynchr

131 assessment of polarity establishment in one-cell stage Caenorhabditis elegans embryos by combining t

132 rtical response to laser ablation in the one-cell-stage Caenorhabditis elegans embryo and in the gast

133 mouse blastomere from an embryo until the 8-cell stage can generate an entire blastocyst.

134 mbranes, and also between cells of different cell stage, cell and tissue types, and organisms.

135 We show that a C. elegans one-cell stage centrosome at metaphase contains >10,000 micr

136 ydrogenase is transiently nuclear at the 4/8-cell stage coincident with timing of human embryonic gen

137 zes to hermaphrodite X chromosomes at the 30-cell stage, coincident with a developmental transition f

138 ed beta-catenin which, by as early as the 32-cell stage, commits nuclei in prospective dorsal lineage

139 ith embryonic genome activation at the eight-cell stage, continuing through the emergence of epiblast

140 nome-wide demethylation is complete at the 2-cell stage, contrary to previous observations in mice.

141 ing and loss of activated STAT5 at the pre-B cell stage corresponds with Igkappa locus accessibility

142 By the early globular ( approximately 32-cell) stage, dcl1-null mutant embryos overexpress approx

143 nalysis of six consecutive neural progenitor cell stages derived from a HES5::eGFP reporter human emb

144 Here, we defined a four-cell-stage developmental framework for Tex cells.

145 go through a Rag(hi) CD79(+)IgH-mu(+) pre-B cell stage, different from mammals.

146 d that blockade of Erk signalling from the 8-cell stage does not impede blastocyst formation but supp

147 petitive element silencing occurs by the two-cell stage, does not require Xist, and occurs several di

148 oxo1 caused a substantial block at the pro-B cell stage due to a failure to express interleukin 7 rec

149 o-B cells resulted in an arrest at the pre-B cell stage due to lower expression of the recombination-

150 uccessfully fertilized eggs die before the 2-cell stage due to persistence of secreted innate immune

151 tion in nuclear organization occurs at the 8-cell stage during C. elegans embryogenesis.

152 After development into the pro-B cell stage, Ebf1 and other genes switched compartments t

153 ection of DNA constructs into fertilized one-cell stage eggs, followed by a low dose of irradiation,

154 ation is asymmetrically regulated in the two-cell stage embryo and that the PAR-4 and PAR-1 polarity

155 on of polarised blastomeres in the 8- and 16-cell stage embryo determines the fate of daughter cells,

156 ories by analyzing the outwardly similar one-cell stage embryo of its close relative Caenorhabditis b

157 In the eight-cell stage embryo, H3K4me3 deposition is poor in the ger

158 a specific cell cycle transition in the one-cell stage embryo.

159 y expressed in the apical membranes of the 8-cell-stage embryo just before morula compaction.

160 n mRNA or proteoglycan/protein core into one-cell stage embryos caused cyclopia.

161 11-33% of 1-cell stage embryos injected with TALEN mRNAs targeting r

162 One-cell stage embryos were injected with NOX4 RNA prior to

163 We discovered that injecting 2-cell stage embryos with a plasmid bearing a varphiC31 in

164 By co-injection of one-cell stage embryos with Cas9 mRNA and Npc1l1 sgRNA, we a

165 ng a guide RNA (gRNA) and Cas9 mRNA into one-cell stage embryos.

166 ), matured oocytes (metaphase II eggs) and 2-cell stage embryos.

167 In two-cell-stage embryos harboring abnormal monopolar spindles

168 Satb1 is differentially expressed within 16-cell-stage embryos, with higher expression levels in the

169 ulating in two nuclear foci in the 64- to 1k-cell-stage embryos.

170 mbination with partial blockade at the pro-B cell stage, Emu deletion (core or full length) did not a

171 nto the yolk of zebrafish embryos at the one-cell stage enables systematic imaging of sialylation and

172 ced in any position at any time after the 32-cell stage exhibited randomized laterality.

173 TADs become weaker at the 2-cell stage, followed by gradual consolidation.

174 Upon injection at the one-cell stage, FRET nanoprobes can be imaged in developing

175 ost severely perturbed at the CD4(+)CD8(+) T-cell stage from which tumors initiate.

176 Zygotes at the 1-cell stage have been genetically modified by microinject

177 n encounter at the Rag-expressing immature B-cell stage helps shape pre-immune BCR repertoires.

178 duced and heterogeneously expressed in the 8-cell-stage human embryos during the major wave of embryo

179 logy analysis of genes activated at the four-cell stage identified categories related to RNA processi

180 Beginning at the four-cell stage, imprinted XCI (iXCI) exclusively silences th

181 ac is depleted from the X even before the 30-cell stage in a DCC-independent manner.

182 repetitive elements are silenced at the two-cell stage in a parent-of-origin-specific manner.

183 before B-lineage commitment at the prepro-B cell stage in adult animals.

184 dT RNA is expressed exclusively at the pro-B cell stage in B6.56R.

185 quiescence was acquired at the follicular B cell stage in both humans and mice.

186 a nearly continuous time scale up to the 350-cell stage in C. elegans embryos.

187 t B-cell development is blocked at the pre-B-cell stage in mice deficient for Mef2c and Mef2d TFs and

188 B cell development past the pro-B cell stage in mice requires the Cul4-Roc1-DDB1 E3 ubiqui

189 n is profoundly blocked beyond the pre-pro-B cell stage in Myb(f/f) Mb1-cre mice.

190 arks and germline transcription at the pro-B cell stage in Rag1-deficient mice.

191 ene expression analyses of the pollen mother cell stage in seven diploid sexual and seven diploid apo

192 s ectopic expression of Sox2 prior to the 16-cell stage in the absence of Yap1, Wwtr1 and Tead4 Inter

193 r earlier in B-cell development at the pro-B-cell stage in the bone marrow.

194 Differentiation to the follicular B cell stage in vitro correlated with surface acquisition

195 ically at the transitional to naive mature B cell stage in WAS subjects.

196 ntiation, without the requirement of a pre-B cell stage in zebrafish.

197 premature AID expression from earliest pro-B-cell stages in B-cell transformation.

198 port that emigration is shifted to earlier B-cell stages in mice with orthotopic breast cancer, spont

199 tablished after the first cell division (two-cell stage) in Caco-2 three-dimensional cultures.

200 esponse to oxidative stress during red blood cell stages, indicative of a protective role seen in oth

201 rogeneous gene expression, as early as the 4-cell stage, initiates cell-fate decisions by modulating

202 vo polarisation of the mouse embryo at the 8-cell stage is directed by Phospholipase C and Protein ki

203 GL transcription is delayed to the mature B-cell stage is presently unknown.

204 mex-3 3'UTR nor protein degradation at the 4-cell stage is strictly required.

205 of the natural repertoire at the immature B cell stage is sufficient to promote editing.

206 o these two lineages during the 8-cell to 32-cell stages is accompanied by a significant amount of ce

207 Labeling of cells of intermediate, immature cell stages is elevated.

208 at the immature B and double-positive (DP) T cell stages is mediated through tonic (foreign antigen i

209 cell development, but its function at late B cell stages is unknown.

210 estered at the lamina, and only at the pre-B cell stage located to central nuclear domains.

211 shortly thereafter, at the approximately 300-cell stage, making XND-1 the earliest zygotically expres

212 evels of H3K9 acetylation at the 2-cell to 8-cell stages, meanwhile, significantly decreased the apop

213 that development arrests prior to the mid-2-cell stage, mediated in part by reductions in beta-caten

214 sa protein accumulates selectively in the 16-cell stage micromeres, and then is restricted to the sma

215 Moreover, as early as the eight-cell stage, miR156-mediated repression of zygotic SPL tr

216 o investigate whether the blastomeres of two-cell-stage mouse embryos can reprogram more differentiat

217 a human embryo until the approximately eight-cell stage (n=85).

218 ent or -independent) subject to the target T-cell stage of activation and its tissue location.

219 coincides with induction of EBF at the Pro-B cell stage of B cell differentiation.

220 -)VpreB(+)CXCR4(+) Consistent with the pro-B-cell stage of B-cell development, microarray analysis re

221 g globin in the liver occurs at a progenitor cell stage of development and is preceded by DNA replica

222 However, cells at the pre-B-cell stage of development did not initiate disease.

223 and is necessary for cleavage beyond the two-cell stage of development.

224 o a partial developmental block at the pre-B cell stage of development.

225 The C. elegans MS blastomere, born at the 7-cell stage of embryogenesis, generates primarily mesoder

226 paternal X chromosome (Xp) at about the four-cell stage of embryonic development.

227 on of RNA Pol II at Ser2 observed at 2- or 4-cell stage of embryos under Padi1 knockdown or inhibitin

228 bryos, is symmetrically localized in the one-cell stage of Protorhabditis group species.

229 rosomes for all cell sizes down to the eight-cell stage of the Caenorhabditis elegans embryo, and it

230 dy, we applied single-cell RNA-seq to the 16-cell stage of the Ciona embryo, a marine chordate and pe

231 and VEGF120 mice at E9.5, the primitive stem cell stage of the neuroepithelium.

232 cations from single cells spanning the 1-128-cell stages of development.

233 against the asexually reproducing red blood cell stages of the parasite, which are responsible for t

234 scent Gata6 and Nanog protein from the eight-cell stage onward before it preferentially cosegregates

235 the CFU-E/proerythroblast (CD71(+) Ter119(-) cells) stage onward, erythroid progenitors exhibited exc

236 to affect zygotic gene activation at the two-cell stage or lineage gene transcription at the morula s

237 The highly correlated gene pairs at the 4-cell stage overlapped with those showing the same direct

238 ntly increased incidences of arrest at the 4-cell stage (p < 0.05).

239 ll-intrinsic mechanism starting at the pro-B cell stage paralleling IL-33 expression.

240 es that proliferation during pro-B and pre-B cell stages plays an important role in the homeostasis o

241 embryos are preimplantation lethal by the 32-cell stage, precluding in vivo study of Geminin's role i

242 intact IL-7 signaling, GON4L-deficient pro-B cell stage precursors failed to undergo a characteristic

243 aging multiple times at the Neural Precursor Cell stage, prior to final differentiation.

244 ary to expectations, outside cells at the 16-cell stage represent a heterogeneous population, with so

245 After the 4-cell stage, residual MEX-3 is degraded in somatic cells,

246 rexpression and knockdown of Dvr1 at the one-cell stage resulted in defects at epiboly and C&E.

247 ete block in B cell development at the pre-B cell stage resulting from a deletion in the Fnip1 gene.

248 transporters are affected as early as the 8 cell stage, resulting in apical redistribution of auxin.

249 ells was impaired at the pre- and immature B cell stage, resulting in decreased numbers of follicular

250 Deletion of Cdc42 from proB/preB-cell stage significantly blocked B-cell development at T

251 By the 8-cell stage, somatic chromatin architecture is largely re

252 r results form a basis for understanding the cell stage-specific roles for CHD7-mediated chromatin re

253 be differentiated to hematopoietic precursor cells, stage-specific analysis of T cell maturation conf

254 ome of the polarization events at the late 8-cell stage such as compaction and apical localization of

255 cell stage, TET3 mRNA remained high at the 4-cell stage suggesting that degradation of TET3 is relate

256 GCB cell to the plasmablast--the transient B-cell stage targeted in ABC-DLBCL transformation--by anta

257 minor differences were detected in the pro-B cell stage tended to diminish with B cell maturation, su

258 cription or translation was blocked at the 2-cell stage, TET3 mRNA remained high at the 4-cell stage

259 opment is arrested at an IgD- transitional B cell stage that we term transitional type 0 (T0).

260 g been known that one blastomere at the four-cell stage, the D cell, and its direct descendants play

261 During its red blood cell stage, the malaria parasite Plasmodium falciparum c

262 We find that at the two-cell stage, the somatic AB cell initiates centrosome sep

263 al distribution was lost, such that by the 2-cell stage there was no evidence of PKA localisation.

264 also execute the chiral skew event at the 4-cell stage to establish the C. elegans LR body axis.

265 trongylocentrotus purpuratus, from the eight-cell stage to late in gastrulation.

266 dynamics of mouse development from the eight-cell stage to postimplantation using lineage-specific RN

267 sis at the transition from the large pre-BII cell stage to the small pre-BII cell stage was exacerbat

268 ion increases from ~60 nJ . s(-1) at the two-cell stage to ~90 nJ . s(-1) at the 1024-cell stage.

269 ifferent cell stages (1-, 2-, 4-, 8-, and 16-cell stage) to investigate the localization changes of s

270 nscriptionally inactive, but at around eight-cell stage transcription is activated in the somatic lin

271 in ES cells activates a totipotent-like two-cell-stage transcriptional program that includes high le

272 mpetent to express Sox2 as early as the four-cell stage, transcriptional repression prevents the prem

273 ur understanding of the parasite's red blood cell-stage transcriptome.

274 hich plays a critical regulatory role during cell stage transitions of mouse embryonic stem cells (mE

275 At the naive T cell stage, two intrinsic checkpoints that actively main

276 evels during mouse development, from the two-cell stage up to gastrulation.

277 and tracking of individual nuclei from the 4-cell stage up to the blastocyst.

278 mbryo development was also arrested at the 4-cell stage upon depletion of PADI1 or inhibition of PADI

279 We find that at the 16-cell stage, very few inside cells are initially produced

280 ling through cytokine receptors at the pro-B-cell stage (via activation of the signal-transduction pr

281 arge pre-BII cell stage to the small pre-BII cell stage was exacerbated by abnormal cytokine signalin

282 To induce deletion of podoplanin at the 2-cell stage, we generated a podoplanin(fl/fl) mouse cross

283 at multipolar divisions at the zygote or two-cell stage were associated with CCFs and generated a ran

284 When blastomeres of 4- to 8-cell stages were dissociated, the polarized MOEP19 domai

285 Embryos (1-4 cell stage) were microinjected with either 1 or 10 ng ve

286 s asymmetry becomes more pronounced at the 4-cell stage, when SKN-1 is high in the posterior cell's d

287 have a developmental block at the pro/pre-B cell stage, whereas a B cell-specific Shp-1 deficiency p

288 to progress toward the transitional type 2 B cell stage, whereas cells that have passed this step gen

289 or developmental progression at the prepro-B cell stage, whereas E12 is dispensable for early B cell

290 observed in all euploid embryos to the four-cell stage, whereas only 30% of aneuploid embryos exhibi

291 n of monocytes and LCs at an early precursor cell stage, whereas progenitor cell expansion or granulo

292 the H chain locus is accessible at the pro-B cell stage, whereas the L chain loci become accessible a

293 lamina to central domains only at the pro-B cell stage, whereas, Igkappa remained sequestered at the

294 negative) to CD4(+)CD8(+) (double positive) cell stages, whereas T cell activation and adhesion are

295 lei of the four vegetal-most cells at the 64-cell stage, which give rise to definitive larval and adu

296 and multiple progenitor (transit-amplifying) cell stages, which ultimately give rise to TD cells.

297 sulted in a developmental block at the pre-B cell stage, with a corresponding lack of peripheral B ce

298 mice results in embryonic arrest at the 1-2 cell stage, with arrested embryos failing to undergo the

299 gregation of chromosomes as early as the two-cell stage, with corresponding high levels of aneuploidy

300 ection of mRNAs encoding these ZFNs into one-cell-stage zebrafish embryos led to mutagenic lesions at