ライフサイエンスコーパス: cellulosome

1 those of the larger, multienzyme complexes (cellulosomes).

2 bility, and higher order organization of the cellulosome.

3 rs to dissociate the oligomeric state of the cellulosome.

4 s a key role in the Clostridium thermocellum cellulosome.

5 protein scaffold forming a complex called a cellulosome.

6 atus of anaerobic microorganisms, termed the cellulosome.

7 r biotechnological development of the fungal cellulosome.

8 g apparatus of anaerobic bacteria termed the cellulosome.

9 icellular eukaryotes, also reside within the cellulosome.

10 tive enzymes were known to reside within the cellulosome.

11 nzyme cellulose-degrading complex called the cellulosome.

12 n enzymatic subunits of the C. cellulovorans cellulosome.

13 ng for CelK, a 98-kDa major component of the cellulosome.

14 n extracellular cellulase complex termed the cellulosome.

15 me and properly integrated into the designer cellulosome.

16 aratus into a large multienzyme complex, the cellulosome.

17 's most elaborate multienzyme complexes, the cellulosome.

18 processes exemplified by the assembly of the cellulosome.

19 f beta-glucosidase would be available to the cellulosome.

20 ay enable new strategies to create designers cellulosomes.

21 ing ways to improve biomass conversion using cellulosomes.

22 -grained model to study the self-assembly of cellulosomes.

23 cell surface and complements the activity of cellulosomes.

24 or role and a minor role in C. cellulovorans cellulosomes.

25 ins was suggested as a model for assembly of cellulosomes.

26 cellulose hydrolysis that are distinct from cellulosomes.

27 t when compared to this pattern of untreated cellulosomes.

28 bled into large multienzyme complexes termed cellulosomes.

29 nt synergies with pivotal cellulases in mini-cellulosomes.

30 syl hydrolases into large complexes known as cellulosomes.

31 in type I dockerins that direct enzymes into cellulosomes.

32 ce for the various catalytic subunits of the cellulosome, a large extracellular cellulase complex.

33 idium thermocellum produces the prototypical cellulosome, a large multienzyme complex that efficientl

34 ciated glycosyl hydrolase into the bacterial cellulosome, a multienzyme cellulolytic complex, via its

35 lulosic biomass either by free enzymes or by cellulosomes, ability to rapidly ferment hexose and pent

36 recruit hemicellulases to the sophisticated cellulosomes acting in the gastrointestinal tract of mam

37 In this study, we complemented a cellulosome active on cellulose and hemicellulose by add

38 acts will provide more detailed insight into cellulosome activity.

39 The dynamic integration of enzymes in cellulosomes, afforded by the dual binding mode, is beli

40 retreated switchgrass compared to the native cellulosome alone or in combination with wild-type BglA

41 LPMOs were able to self-assemble in designer cellulosomes alongside an endo- and an exo-cellulase als

42 Consequently, a model of the entire cellulosome and a detailed picture of intermodular conta

43 Complexation of the cellulosome and BglA-CohII resulted in higher overall de

44 EngF is not a component of the cellulosome and does not contain a duplicated sequence (

45 e CipA, which is a structural protein of the cellulosome and has a strong affinity for calcium.

46 Comparison of cellulosome and noncellulosomal enzymes showed that expr

47 e modular versatility of the R. flavefaciens cellulosome and sheds light on the mechanisms that enhan

48 of enzyme recruitment into B. cellulosolvens cellulosome and the identification of the molecular dete

49 Ca is incorporated into the cellulosome and tightly bound as demonstrated using 45Ca

50 p, changes in mRNA and protein expression in cellulosomes and noncellulosomal (hemi)cellulolytic enzy

51 Cellulosomes and noncellulosomal (hemi)cellulolytic enzy

52 lable carbon source and that synergy between cellulosomes and noncellulosomal enzymes contribute to p

53 Cellulosomes and noncellulosomal enzymes had synergistic

54 scription and protein analyses revealed that cellulosomes and noncellulosomal enzymes were expressed

55 dockerin modules, the basic elements of the cellulosome, and manual sequencing of partially sequence

56 lulosomal protease inhibitors would regulate cellulosome architecture and then lignocellulose hydroly

57 The basic elements of its cellulosome architecture, mainly cohesin and dockerin mo

58 ducing bacteria, but reveal a more organized cellulosome architecture.

59 lusters comprising fundamental components of cellulosome architecture.

60 from the proximity effects generated by the cellulosome architecture.

61 ng profiles which implied two major types of cellulosome architectures: (i) an intricate cell-bound s

62 Since gene clusters for the cellulosome are also found in C. cellulolyticum and C. j

63 Cellulosomes are assembled by selective high-affinity bi

64 Cellulosomes are key for lignocellulosic biomass degrada

65 Cellulosomes are large, multienzyme, plant cell wall-deg

66 Cellulosomes are large, multiprotein complexes that teth

67 Cellulosomes are multienzyme complexes responsible for e

68 Cellulosomes are polyprotein machineries that efficientl

69 Cellulosomes are produced exclusively by anaerobic micro

70 ABTRACT: Cellulosomes are sophisticated multi-enzymatic nanomachi

71 Cellulosomes are typically recruited to the cell surface

72 XynY and XynZ, components of the cellulosome, are composed of several domains including x

73 nd to be a component of the C. cellulovorans cellulosome as well as the previously reported endogluca

74 The Clostridium thermocellum cellulosome assembles through the interaction of a type

75 ockerin-bearing ORFs potentially involved in cellulosome assembly and a variety of multi-modular scaf

76 Thus, this work reveals a novel mechanism of cellulosome assembly and challenges the ubiquitous impli

77 w dockerin-cohesin interactions mediate both cellulosome assembly and the retention of the protein co

78 Cellulosome assembly involves the binding of enzyme-born

79 racterization of the factors influencing the cellulosome assembly process may enable new strategies t

80 rehensive set of proteins critical to fungal cellulosome assembly, including conserved scaffolding pr

81 In the canonical model of cellulosome assembly, type I dockerin modules of the enz

82 e contains a dockerin domain responsible for cellulosome assembly.

83 ues, is proposed, providing new insight into cellulosome assembly.

84 increased conformational flexibility during cellulosome assembly.

85 imary scaffoldin, and type I modules mediate cellulosome attachment to an anchoring scaffoldin.

86 obal exploration of PTMs associated with the cellulosome based upon a set of defined PTMs that includ

87 to the surface of C. thermocellum and not to cellulosomes because it binds preferentially to type I c

88 me will be useful for rumen microbiology and cellulosome biology and in biofuel production, as one of

89 e to the integration of enzymes into primary cellulosomes but can also characterize polycellulosome a

90 rin module and were formerly detected in the cellulosomes, but only three of them were previously stu

91 t the intriguing possibility that individual cellulosomes can associate with one another via inter-sc

92 We reveal that bacterial cells form 'cellulosome capsules' driven by catalytic product-depend

93 eviously, a dual-binding mode is involved in cellulosome cell-surface attachment, whereas single-bind

94 ar mechanisms used by anaerobic bacteria for cellulosome cellular attachment.

95 rporation of all GH9 cellulases in trivalent cellulosome chimera containing Cel48F and Cel9G generate

96 ern was investigated in the free state or in cellulosome chimeras with key cellulosomal cellulases.

97 In contrast to other known cellulosomes, cohesin types from Bacteroides cellulosolv

98 of binding both to CbpA to form a CbpA-EngE cellulosome complex and to the surface layer of C. cellu

99 iens produces a highly organized multienzyme cellulosome complex that plays a key role in the degrada

100 ellulose degradation activity by binding the cellulosome complex to the cellulose substrate; secondar

101 arrangement of the enzymatic subunits in the cellulosome complex, made possible by the scaffoldin sub

102 izes cellulose with the aid of a multienzyme cellulosome complex.

103 rocedures did not involve a clostridium-like cellulosome complex.

104 ed by assembly of the intricate multi-enzyme cellulosome complex.

105 and was readily incorporated into the native cellulosome complex.

106 d to the cell surface and involves elaborate cellulosome complexes in specialized cellulolytic specie

107 e of HLDs on cellulose degradation, the mini-cellulosome complexes with HLDs degraded cellulose more

108 crucial role in the assembly of multienzyme cellulosome complexes.

109 ression of xylanase activity and to vary the cellulosome composition depending on the growth substrat

110 on for future studies of biomass conversion, cellulosome composition, and clostridial systems biology

111 rio cellulolyticus produces a highly complex cellulosome comprising an unusual adaptor scaffoldin, Sc

112 yed pivotal roles in describing the original cellulosome concept.

113 the importance of GH9 diversity in bacterial cellulosomes, confirm that Cel9G is the most synergistic

114 complex of Clostridium thermocellum, termed cellulosome, consists of up to 26 polypeptides, of which

115 e degradation by three different recombinant cellulosomes containing either endoglucanase EngE, endog

116 The mixture of the cellulosomes containing ExgS and EngH showed higher acti

117 ion of Clostridium cellulovorans recombinant cellulosomes containing the enzymatic subunit EngB and t

118 n addition to a large collection of integral cellulosome-containing proteins.

119 caffoldin and what governs the population of cellulosomes created during self-assembly.

120 e gel electrophoresis analysis revealed that cellulosomes derived from xylan-, cellobiose-, and cellu

121 ciens FD-1 harbors one of the most intricate cellulosomes described to date, comprising over 200 Doc-

122 Therefore, in contrast to all cellulosomes described to date, the assembly of R. flave

123 species sequenced fall within the class III cellulosome-encoding Clostridium and the class V sacchar

124 it was found that the native C. thermocellum cellulosome exists as a homooligomer and the high-affini

125 ioinformatic analyses of the R. flavefaciens cellulosome failed to identify a CBM predicted to bind t

126 the dual-binding mode in the acquisition of cellulosome flexibility.

127 Several mechanisms involved in cellulosome formation remain unknown, including how cell

128 lot analysis was carried out with the native cellulosome fraction bound to cell wall fragments, the p

129 Purified cellulosomes from C. thermocellum were found to hydrolyz

130 Also, when xylanase activity in the cellulosomes from the xylan-grown cultures was compared

131 ngB, one component of the cellulase complex (cellulosome) from Clostridium cellulovorans, by the dire

132 n the catalytic subunits is a key feature of cellulosome function.

133 A comparison of the glycoside hydrolase and cellulosome functional genes revealed that in the rumen

134 ne cluster for the Clostridium cellulovorans cellulosome has been cloned and sequenced upstream and d

135 or subunits of the Clostridium cellulovorans cellulosome, has been cloned and sequenced.

136 or subunits of the Clostridium cellulovorans cellulosome, has been determined.

137 or subunits of the Clostridium cellulovorans cellulosome, has been isolated and sequenced.

138 Recently, designer cellulosomes have been developed to incorporate foreign

139 The intact cellulosomes hydrolyzes crystalline cellulose in the pre

140 nism for covalent cell-wall anchoring of the cellulosome in R. flavefaciens differs from those report

141 assembly and cell surface attachment of the cellulosome in R. flavefaciens, we determined the crysta

142 ions were also performed by adding different cellulosomes in a sequential manner.

143 sible for the structural organization of the cellulosome, including olpB, orf2p, and olpA.

144 reas single-binding interactions operate for cellulosome integration of enzymes.

145 bed to date, the assembly of R. flavefaciens cellulosome involves single but not dual-binding mode Do

146 The cellulosome is a multiprotein complex, produced primaril

147 The core of the cellulosome is a noncatalytic scaffoldin protein, which

148 nzyme beta-glucosidase; however, because the cellulosome is adsorbed to the insoluble substrate only

149 These findings suggest that the fungal cellulosome is an evolutionarily chimaeric structure-an

150 The Cellulosome is an intricate macromolecular protein compl

151 Ruminococcus flavefaciens' cellulosome is assembled from a repertoire of 223 Doc-co

152 responsible for the enzymatic synergy of the cellulosome is hindered by the large size and inherent f

153 A central feature of bacterial cellulosomes is a large non-catalytic protein, the scaff

154 he biocatalytic activity of anaerobic fungal cellulosomes is expanded by the inclusion of GH3, GH6 an

155 ent dockerins to assemble the most intricate cellulosome known to date.

156 y identified the physiological importance of cellulosome-localized protease inhibitors in Clostridia.

157 northodox use of aerobic enzymes in designer cellulosome machinery effects simultaneous degradation o

158 exibility in the quaternary structure of the cellulosome may reflect the challenges presented by the

159 activity and synergy degrees than the other cellulosome mixtures, indicating the synergistic effect

160 This sui generis cellulosome model enhances our understanding of the mech

161 es of the solventogenesis pathway and of the cellulosome of C. acetobutylicum comprise a new set of m

162 The cellulosome of Clostridium cellulovorans consists of thr

163 The cellulosome of Clostridium thermocellum is a multipolype

164 The cellulosome of Clostridium thermocellum is a multiprotei

165 The cellulosome of Clostridium thermocellum JW20 consists of

166 t the extended repertoire of proteins in the cellulosome of R. flavefaciens contributes to an extende

167 During the course of evolution, the cellulosome, one of Nature's most intricate multi-enzyme

168 s play a pivotal role in the assembly of the cellulosome, one of nature's most intricate nanomachines

169 ell envelope proteins, thereby anchoring the cellulosome onto the bacterial cell.

170 This report reveals that recruitment of cellulosomes onto the cell surface may involve dockerins

171 n associated with microbial surface-tethered cellulosomes or secreted cellulase enzymes to enhance su

172 al for the in vitro assembly of a "designer" cellulosome, or a recombinant cellulosome with a specifi

173 our results highlight the importance of the cellulosome paradigm for cellulose and hemicellulose deg

174 These studies are the first to expand the cellulosome paradigm of protein complex assembly beyond

175 folding protein of Clostridium cellulovorans cellulosomes, possesses one family 3 cellulose binding d

176 ecause the large size and flexibility of the cellulosome preclude structural determination by traditi

177 The cellulosome produced by the S mutant strain was purified

178 In cellulosomes produced by Clostridium spp., the high-affi

179 with fibronectin domains similar to those in cellulosome-producing bacteria were also abundant, sugge

180 icity types, as generally believed for other cellulosome-producing bacteria, but reveal a more organi

181 sin types from Bacteroides cellulosolvens, a cellulosome-producing bacterium capable of utilizing cel

182 Clostridium thermocellum is a cellulosome-producing bacterium that couples rapid repro

183 contains the genetic elements necessary for cellulosome production.

184 e of post-translational modifications of the cellulosome protein complex used by the bacterium Clostr

185 ehensive characterization, the extracellular cellulosome proteins were analyzed using multiple proteo

186 ts, 85 modified peptides corresponding to 28 cellulosome proteins were identified.

187 ve cellulolytic or structural domains of the cellulosome proteins, suggesting a level of possible reg

188 t was observed that about 15 subunits of the cellulosome reacted with anti-bCDR1 and anti-bCDR2.

189 Better understanding of the mechanism of cellulosome regulation would allow us to improve lignoce

190 mbly mechanism and functional role of fungal cellulosomes remain unknown(5,6).

191 's most elaborate multienzyme complexes, the cellulosome, results from the binding of enzyme-borne do

192 es, depending on structural conformation and cellulosome role.

193 incorporate foreign enzymatic activities in cellulosomes so as to enhance lignocellulose hydrolysis

194 mplex represents the largest fragment of the cellulosome solved by x-ray crystallography to date and

195 rt of a gene cluster that also codes for the cellulosome structural components ScaA and ScaB.

196 assembly of the catalytic subunits into the cellulosome structure.

197 observed and may play a role in higher order cellulosome structure.

198 ions that assemble functional multienzymatic cellulosome structures capable of degrading plant cell w

199 minus of the CipA scaffoldin that may aid in cellulosome substrate recognition and binding.

200 wn as CelS, S(S), and S8), the most abundant cellulosome subunit, was undertaken to gain insight into

201 bacterial cellulase systems, the multienzyme cellulosome system of the anaerobic, cellulolytic bacter

202 The cellulosome system of the human gut bacterium R. champan

203 in the human gut that produces an intricate cellulosome system of various architectures.

204 We test our model against the biological cellulosome system.

205 s from those reported thus far for any other cellulosome system.

206 D-1 genome revealed a particularly elaborate cellulosome system.

207 e-dockerin construct to bind more tightly to cellulosomes than a single domain and with greater cover

208 in complex from the Clostridium thermocellum cellulosome that comprises a C-terminal trimodular fragm

209 9G generated a mixture of heterogeneous mini-cellulosomes that exhibit more activity on crystalline c

210 Despite substantial research on this cellulosome, the role of a group of truncated but functi

211 ntributes to the assembly of R. flavefaciens cellulosome, the structures of two group 1 Docs bound to

212 Particularly fascinating are cellulosomes, the multimodular extracellular complexes p

213 In a similar experiment with EDTA-treated cellulosomes, these subunits reacted with anti-bCDR1 but

214 ures of all of the individual modules of the cellulosome, this mechanism of enzymatic synergy remains

215 dase (BglA-CohII) that binds directly to the cellulosome through an unoccupied dockerin module of its

216 f digestive enzymes is incorporated into the cellulosome through the interaction of the dockerin doma

217 t also that EngE plays an anchoring role for cellulosomes through the interaction of its dockerin dom

218 , while type II Coh-Doc complexes tether the cellulosome to the bacterial wall.

219 econdarily, HLDs aid the binding of the CbpA/cellulosome to the C. cellulovorans cell surface.

220 he HLDs of CbpA play a role in attaching the cellulosome to the cell surface, since they showed some

221 ay a secondary role in the attachment of the cellulosome to the cell surface.

222 rete multi-component protein networks called cellulosomes to effectively digest lignocellulosic bioma

223 lent modes of cohesin recognition to recruit cellulosomes to the cell surface, a mechanism that maxim

224 ot form large polypeptide structures, called cellulosomes, to deconstruct biomass.

225 of feruloyl esterase in the C. thermocellum cellulosome together with its other hydrolytic activitie

226 rmit improved understanding of the bacterial cellulosome using biophysical approaches, with emphasis

227 e anchoring in the Ruminococcus flavefaciens cellulosome using single-molecule force spectroscopy and

228 Docs of groups 3 and 6 are recruited to the cellulosome via a single-binding mode mechanism with an

229 The composition of the purified cellulosome was analyzed by tandem mass spectrometry wit

230 C. cellulovorans native cellulosomes were found to have higher affinity for cell

231 caG), thus enabling formation of a cell-free cellulosome, whereby ScaG interacts with a divalent scaf

232 ture produce a multiprotein complex called a cellulosome, which co-ordinates glycoside hydrolase asse

233 xtracellular multiprotein complex termed the cellulosome, which consists of >70 subunits, most of the

234 DTA at 37 degrees C, Ca is released from the cellulosome, which disintegrates into polypeptides.

235 al role in the assembly of B. cellulosolvens cellulosome, which is consistent with its unmatched comp

236 extracellular multienzyme complex called the cellulosome, which is involved in plant cell wall degrad

237 GH3 beta-glucosidase component of the fungal cellulosome, which is thereby identified as a potential

238 ophisticated multi-enzyme complexes known as cellulosomes, which enable the efficient breakdown of th

239 cteria use large enzymatic assemblies called cellulosomes, which recruit complementary enzymes to pro

240 d into large multienzymes complexes, termed "cellulosomes," which allow for efficient hydrolysis of c

241 responsible for enzyme integration into the cellulosome, while type II Coh-Doc complexes tether the

242 f a "designer" cellulosome, or a recombinant cellulosome with a specific function.

243 hown in our previous paper, treatment of the cellulosome with ethylenediaminetetraacetic acid (EDTA)

244 ) under aerobic conditions disintegrates the cellulosome with formation of truncated catalytic subuni