ライフサイエンスコーパス: centriolar

1 ent to the mammalian sperm manchette, to the centriolar adjunct and acrosomal cap during spermiogenes

2 hat gamma-tubulin is mainly localized in the centriolar adjunct from which an aster of microtubules e

3 Centriolar ALMS1 expression was retained into maturity.

4 Here we demonstrate that the centriolar and basal body protein HYLS-1, the C. elegans

5 e formation, PLK4, associated with increased centriolar and centrosomal protein levels, endow mESCs w

6 ence centrosomes possess increased levels of centriolar and pericentriolar components including gamma

7 Thus, both centriolar and pericentriolar material proteins contribu

8 -tubulin is a component of the basal foot, a centriolar appendage that connects centrioles to the api

9 indicates that centriolin localizes Evi5 to centriolar appendages to turn off centrosomal Rab11 acti

10 tures were present, supporting the idea that centriolar assembly starts with the formation of a tube-

11 Our findings reveal how two centriolar cap proteins with opposing activities regulat

12 Assembly of SAS-6 dimers to form the centriolar cartwheel requires the ZYG-1/Plk4 kinase.

13 EP19 is recruited to the ciliary base by the centriolar CEP350/FOP complex and then specifically capt

14 ils of ultrastructural organization, such as centriolar chirality, that could otherwise be observed o

15 Centriolar coiled coil protein 110 (CP110) caps the dist

16 We show that cea encodes the centriolar coiled-coil protein Sas-6, and that zebrafish

17 trated that an Ana2-dynein light chain (LC8) centriolar complex is critical for proper spindle positi

18 nas, mutation of the gene encoding the novel centriolar component Bld10p results in seemingly acentri

19 investigate the molecular role of the mother centriolar component Cep164 in ciliogenesis.

20 d that the centrosomal protein 83 (CEP83), a centriolar component necessary for primary cilia formati

21 w study shows that PLK4 phosphorylates a key centriolar component, Ana2/STIL, to initiate centriole a

22 ntrioles that elongate normally and localize centriolar components correctly.

23 proximity and CDK1-CyclinB interaction with centriolar components, ensure that centriole biogenesis

24 Centrosomes are composed of a centriolar core surrounded by a pericentriolar material

25 Centrosomes are composed of a centriolar core surrounded by pericentriolar material th

26 bin-depleted cells restored the cellular and centriolar CPAP expression, suggesting its ubiquitinatio

27 in-depleted cells led to the reappearance of centriolar CPAP.

28 somal protein 4.1 epitopes distributed along centriolar cylinders and on pericentriolar fibers, at le

29 erm shapes the developmental consequences of centriolar defects and p53 activation.

30 tegrity and insights into diseases linked to centriolar defects.

31 our knowledge, we have identified the first centriolar deubiquitinating enzyme whose expression regu

32 and is required for the recruitment of five centriolar distal appendage proteins: Sclt1, Ccdc41, Cep

33 lium biogenesis by promoting the assembly of centriolar distal appendages critical for docking ciliar

34 iates, and, as maturation progressed, mother centriolar docking at the plasma membrane.

35 This extra centriolar DSas-6/Ana2 induces centriole overduplication

36 Subdistal appendages (sDAPs) are centriolar elements that are observed proximal to the di

37 s a centrosomal protein possibly involved in centriolar elongation and ciliogenesis.

38 rting endosomes and accumulation in the peri-centriolar endocytic recycling compartment takes place n

39 and promotes the generation of supernumerary centriolar foci, whereas ablation of USP33 destabilizes

40 Overall, identification of a non-centriolar function of CPAP in endocytic trafficking pro

41 ison to cells lacking Ift88 reveals that the centriolar functions of Kif3a are independent of IFT.

42 ith a change in the primary structure of the centriolar inner scaffold protein FAM161A in rodents.

43 epletion of Cep76 drives the accumulation of centriolar intermediates in certain types of cancer cell

44 letion of Tssk1 and 2 (companion paper) this centriolar kinase/substrate pair is predicted to play an

45 Thus, our studies have identified a centriolar kinesin that specifically remodels a subset o

46 omal proteins that play a role in regulating centriolar length and ciliogenesis.

47 In addition, MNR, but not CEP90, restricts centriolar length by recruiting OFD1.

48 bin from cells did not have an effect on the centriolar levels of CEP152, it caused the disappearance

49 a syndromic PPP2R3C variant is defective in centriolar localization and binding to centriolar protei

50 ain, a C-terminal dimerization domain, and a centriolar localization domain.

51 In addition to the centriolar localization during flagellogenesis, mouse TS

52 We confirmed centriolar localization for the human homologs of four c

53 es SPD-2 centriolar recruitment, while SAS-7 centriolar localization is SPD-2-independent.

54 resses centriole duplication by limiting the centriolar localization of CEP135(full) binding proteins

55 Notably, the centriolar localization of FAM92A and -92B depends large

56 ughter centriole formation and regulates the centriolar localization of the other components in C. el

57 fulfills these diverse functions by ensuring centriolar localization of WDR90, recruiting the protein

58 This centriolar localization persisted in ejaculated human sp

59 However, the molecular basis for SPD-2 centriolar localization remains unknown.

60 ate "in-to-out" molecular hub connecting the centriolar lumen, distal microtubule cap, and appendages

61 The clear absence of several centriolar markers in mecD mutants suggests that Asl is

62 two such centrioles nevertheless retain the centriolar markers mEGFP::PACT and pmPoc1::mEGFP.

63 Using fluorescent centriolar markers, we identified a structure near the f

64 AP, negatively regulates CPAP-dependent peri-centriolar material recruitment, and concurrently microt

65 e proteins are generally thought to suppress centriolar microtubule growth, suggesting that distal ti

66 ld proteins that ensure the integrity of the centriolar microtubule wall.

67 centrosomal substructures, which include the centriolar microtubule walls, cartwheel, inner scaffold,

68 tal appendages, recruit Ift88, and stabilize centriolar microtubules at a defined length.

69 ond the distal end of the centriole, as some centriolar microtubules can be more than 50 times longer

70 Second, CP110 ensures that the centriolar microtubules do not extend beyond the distal

71 4, which is required for the assembly of the centriolar microtubules that decorate that tube [4, 5].

72 A also acts as a microtubule depolymerase on centriolar microtubules to regulate centriole length.

73 ically expressed Kif24 specifically remodels centriolar microtubules without significantly altering c

74 Centriolar microtubules, unlike their cytosolic counterp

75 chestrate the assembly of spatially distinct centriolar modules, and provide a framework for dissecti

76 er that correlate with the presence of other centriolar modules, including the pinhead, cartwheel, an

77 triole assembly involves the coordination of centriolar modules.

78 rchitecting the Cep63-Cep152 assembly around centriolar MTs and promoting centriole biogenesis.

79 cells, identifying a potential link between centriolar or ciliary function and mesodermal lineage in

80 Although Plk4's centriolar partners and mechanisms that regulate its sta

81 lved to relieve, rather than supplement, the centriolar pathway during multiciliogenesis.

82 s, whereas a smaller number grow through the centriolar pathway in association with the two parent ce

83 We describe that the outer centriolar plaque and its associated proteins anchor the

84 le maintaining an association with the outer centriolar plaque during cytokinesis.

85 ted and maintain an association to the outer centriolar plaque until the start of segmentation.

86 ores in relation to the mitotic spindle, the centriolar plaque, the centromeres and the apical organe

87 ochondrion, and showed putative roles of the centriolar plaques in apicoplast segregation.

88 s multiple nuclei, in close association with centriolar plaques.

89 PLK4 activation as well as stabilization of centriolar PLK4 and plays a key role in centriole duplic

90 ppendage assembly, indicating that it is the centriolar populations of MNR and CEP90 that are critica

91 not affect Fz/PCP establishment, implicating centriolar positioning as a conserved PCP-readout, likel

92 ization drives PPM1H substrate selection and centriolar PPM1H is critical for regulation of Rab GTPas

93 e splitting reduces the local density of key centriolar precursors to impede overduplication.

94 Here, we characterize these centriolar protein assemblies (Cenpas) to uncover the me

95 Here we show that the centriolar protein Asterless (Asl; human orthologue CEP1

96 three Mps1 phosphorylation sites within the centriolar protein Centrin 2 (Cetn2).

97 new study has shed light on the role of the centriolar protein centrin-2: reducing levels of centrin

98 ls results in supernumerary foci bearing the centriolar protein Centrin.

99 al body of cilia via an interaction with the centriolar protein CEP19 before downstream association w

100 The centriolar protein Cp110 is a regulator of this process

101 ve in centriolar localization and binding to centriolar protein FOP, we propose that imbalanced activ

102 ied a rare missense variant (p.A446T) in the centriolar protein gene POC5 that cosegregated with the

103 . brucei lacks many evolutionarily conserved centriolar protein homologs and constructs the basal bod

104 lethalus syndrome protein HYLS-1 is the only centriolar protein known to remain at the base of mature

105 We identified the mother centriolar protein ODF2 as an interaction partner of MAR

106 mutation in the gene encoding the conserved centriolar protein POC1, which is part of the daughter c

107 reas the carboxy terminus interacts with the centriolar protein Sas-4 (CPAP in humans).

108 ch MTOCs had pericentriolar material and the centriolar protein Sas-4, but no centrioles at their cor

109 ased on its direct interaction with the core centriolar protein SAS-4.

110 icrocephaly protein CenpJ and the C. elegans centriolar protein Sas-4.

111 ication involves self-oligomerization of the centriolar protein SAS-6, but how the 9-fold symmetry is

112 rganized through homo-oligomerization of the centriolar protein SAS-6, but whether SAS-6 self-assembl

113 embly, involving self-oligomerization of the centriolar protein SAS-6.

114 blasts) that the mitotic kinase Polo and its centriolar protein substrate Centrobin (Cnb) accumulate

115 post-transcriptional repression of Cp110, a centriolar protein suppressing cilia assembly.

116 tii Bld10p and human Cep135, is a ubiquitous centriolar protein that also localizes to the spermatid

117 We have identified Cep76, a centriolar protein that interacts with CP110.

118 oles, defining the first stably incorporated centriolar protein that is not required for centriole as

119 uplication is controlled in part by CP110, a centriolar protein that positively regulates centriole d

120 We use this assay to characterize SAS-6, a centriolar protein that we identified based on its requi

121 se haplotype in CEP120, which encodes a core centriolar protein.

122 in-containing protein TbRP2 is a basal body (centriolar) protein essential for axoneme formation in t

123 Thus, Ana3 defines a conserved family of centriolar proteins and plays an important part in ensur

124 driven by an interaction with the conserved centriolar proteins Asl (Cep152 in humans) and DSpd-2 (C

125 centriole protein and functional partner of centriolar proteins CEP350 and FOP.

126 pathogenesis of JATD and expand the role of centriolar proteins in skeletal ciliopathies.

127 to calculate the average toroidal radius of centriolar proteins in the approximately 20-60 nm range

128 ggesting that Cetn2 can organize a subset of centriolar proteins independently of cartwheels.

129 ic cells, however knowledge regarding gamete centriolar proteins is limited.

130 Second, the centriolar proteins SAS-6, Ana1, and Bld10p/Cep135 are i

131 functions for these important basal body and centriolar proteins.

132 t of mitosis through phosphorylation of core centriolar proteins.

133 s and mathematical modelling indicate that a centriolar pulse of Polo activity, potentially generated

134 ch Plk4 inactivates the interaction with its centriolar receptor through multiple rounds of phosphory

135 for centriole distal appendage formation and centriolar recruitment of the intraflagellar transport p

136 w that SAS-7 binds SPD-2 and regulates SPD-2 centriolar recruitment, while SAS-7 centriolar localizat

137 ophages lack a morphologically distinct peri-centriolar recycling compartment but instead demonstrate

138 LIP-50 protein localizes specifically to the centriolar region where the sperm tail originates and to

139 uggested that pharmacological stimulation of centriolar reproduction without subsequent mitosis may l

140 Centriolar SAS-4 remains in dynamic equilibrium with the

141 Centriolar SAS-6 is subsequently reduced by a mechanism

142 iary vesicle formation through regulation of centriolar satellite accretion and Rab8a.

143 in centriole duplication, ciliogenesis, and centriolar satellite biogenesis and highlights extensive

144 These results suggest that FOP is a centriolar satellite cargo protein and, as for several o

145 The centriolar satellite component PCM-1 colocalized with ce

146 entrosome including microtubule binding, the centriolar satellite component PCM-1, and localized prot

147 analyses showed that Talpid3 is required for centriolar satellite dispersal, which precedes the forma

148 GABARAP instability is mediated through the centriolar satellite E3 ligase Mib1, which interacts wit

149 ired for ciliogenesis but is dispensable for centriolar satellite function.

150 phosphorylates CEP131 at Ser-78 to maintain centriolar satellite integrity.

151 nt manner; however, C2cd3 is dispensable for centriolar satellite integrity.

152 ics with pericentriolar material 1 (PCM1), a centriolar satellite protein crucial for targeting prote

153 entrosomal activity or downregulation of the centriolar satellite protein PCM-1 affects axon formatio

154 olar satellites where it interacted with the centriolar satellite protein PCM-1 and the dynactin subu

155 we demonstrate that CEP290 interacts with a centriolar satellite protein PCM-1, which is implicated

156 ma-tubulin ring complex (gamma-TuRC) and the centriolar satellite protein PCM-1.

157 We show that the centriolar satellite protein PCM1 regulates the recruitm

158 Modulating LIR binding specificity of the centriolar satellite protein PCM1, implicated in autopha

159 drome type 1 protein (OFD1), a centriole and centriolar satellite protein, at K931.

160 Here we characterize the function of a novel centriolar satellite protein, synovial sarcoma X breakpo

161 nown centrosome protein with homology to the centriolar satellite proteins FOR20 and OFD1.

162 ntrosome homeostasis by timely degrading key centriolar satellite proteins PCM1 and CEP290 via HSC70

163 CMA factor LAMP2A exhibit elevated levels of centriolar satellite proteins, which causes aberrant mit

164 vealed extensive proximity interactions with centriolar satellite proteins.

165 MCPH proteins interact with distinct centriolar satellite proteins; CDK5RAP2 interacts with S

166 Quantitative MS on whole-cell and centriolar satellite proteomes of acentriolar cells was

167 y and function of motile cilia and implicate centriolar satellite-associated proteins as a new class

168 Centriolar satellites (CS), membrane-less granules aroun

169 calization occurs normally in the absence of centriolar satellites (PCM1 depletion) but is impaired b

170 Moreover, centriolar satellites also regulate the quantity of prot

171 These results demonstrate that centriolar satellites and centrosomes form independently

172 les colocalize in part with PCM-1 containing centriolar satellites and depletion of PCM-1 interferes

173 /SSX2IP localizes to both the centrosome and centriolar satellites and is required for tethering micr

174 end-directed kinesin motor Kif9 localizes to centriolar satellites and regulates their pericentrosoma

175 ignificance of spatial communication between centriolar satellites and the centrosome is unknown.

176 Centriolar satellites are centrosome-associated structur

177 Centriolar satellites are cytoplasmic particles found in

178 Our data suggest that PCM-1-containing centriolar satellites are involved in the microtubule- a

179 Centriolar satellites are membraneless granules that loc

180 Centriolar satellites are numerous electron-dense granul

181 Centriolar satellites are proteinaceous granules that ar

182 On hMsd1/SSX2IP knockdown, the centriolar satellites become stuck at the microtubule mi

183 Thus, centriolar satellites build a MCPH complex critical for

184 Loss of centriolar satellites by depletion of PCM1 causes reloca

185 ep72, and Cep290 and find that disruption of centriolar satellites by overexpression of Cep72 results

186 These data suggest a mechanism for how centriolar satellites can specifically regulate an ATG8

187 Furthermore, PCM1-GABARAP-positive centriolar satellites colocalize with forming autophagos

188 We propose that PCM1 and centriolar satellites facilitate efficient trafficking o

189 These results suggest that centriolar satellites have a previously unappreciated fu

190 Although centriolar satellites have been implicated in protein tr

191 Although cytoplasmic granules called centriolar satellites have been observed around these st

192 The neuronal primary cilium and centriolar satellites have functions in neurogenesis, bu

193 We show that C2cd3 is localized to the centriolar satellites in a microtubule- and Pcm1-depende

194 CEP290 binds to PCM-1 and localizes to centriolar satellites in a PCM-1- and microtubule-depend

195 To elucidate the role of centriolar satellites in ciliogenesis, we deleted the ge

196 plex, the BBSome, localizes to nonmembranous centriolar satellites in the cytoplasm but also to the m

197 the centrosome and reveal roles for Kif9 and centriolar satellites in the regulation of cellular prot

198 Finally, we show that loss of centriolar satellites in zebrafish leads to phenotypes c

199 pid3 resulted in an aberrant distribution of centriolar satellites involved in protein trafficking to

200 We show that the population of OFD1 at the centriolar satellites is rapidly degraded by autophagy u

201 calization of the FOP-FGFR1 fusion kinase to centriolar satellites may be relevant to myeloproliferat

202 some, suggesting that their association with centriolar satellites normally restricts their centrosom

203 pectrometry (MS)-based proteome profiling of centriolar satellites obtained by affinity purification

204 d OFD1 protein stability and localization at centriolar satellites of primary cilia and decreased cil

205 More strikingly, OFD1 depletion at centriolar satellites promotes cilia formation in both c

206 n, OFD1 (oral-facial-digital syndrome 1), at centriolar satellites promotes primary cilium biogenesis

207 We propose that Cep290 and Cep72 in centriolar satellites regulate the ciliary localization

208 at the kinesin Kif9 controls the position of centriolar satellites relative to the centrosome and rev

209 reveals that removal of OFD1 by autophagy at centriolar satellites represents a general mechanism to

210 During ciliogenesis, BBS4 relocalizes from centriolar satellites to the primary cilium.

211 eloproliferative neoplasm, also localizes to centriolar satellites where it increases tyrosine phosph

212 We detected Par6alpha at the centrosome and centriolar satellites where it interacted with the centr

213 localizes to cytoplasmic granules known as "centriolar satellites" that are partly enriched around t

214 Centrosomes are orbited by centriolar satellites, dynamic multiprotein assemblies n

215 e embryonic fibroblasts, OFD1 accumulates at centriolar satellites, leading to fewer and shorter prim

216 Centriolar satellites, membrane-less assemblies of prote

217 around centrosomes and with CEP131-positive centriolar satellites, promoting CEP131 localization to

218 plex localizes to both distal centrioles and centriolar satellites, proteinaceous granules surroundin

219 cells also have an array of granules, termed centriolar satellites, that localize around the centroso

220 To study the requirement for centriolar satellites, we generated mice lacking PCM1, a

221 ation, accompanied by reduced density of the centriolar satellites, with reexpression of C-NAP1 rescu

222 , translation is enriched at centrosomes and centriolar satellites, with UNK and CEP131 promoting thi

223 onstrate that Ccdc11 is a novel component of centriolar satellites-cytoplasmic granules that serve as

224 lar transport, pericentrosomal material, and centriolar satellites-decreased.

225 the ciliopathy-associated protein Cep290 to centriolar satellites.

226 ylatable CEP131 variant results in dispersed centriolar satellites.

227 osphomimetic variant promoted aggregation of centriolar satellites.

228 al, GABARAP marks a subtype of PCM1-positive centriolar satellites.

229 rity, centrosome length and orientation, and centriolar satellites.

230 the cellular and structural complexities of centriolar satellites.

231 kdown that reduces the population of OFD1 at centriolar satellites.

232 s essential for maintaining the integrity of centriolar satellites.

233 We show that centriolar singlet microtubules are converted into BB do

234 centriole, which may explain why no typical centriolar structure is observed under electron microsco

235 o the periphery of the centrosome but not at centriolar structures as in mammals.

236 interacts with the inner scaffold, a crucial centriolar subcompartment for centriole size control and

237 o centrioles, which is also mediated by OFD1 centriolar translocation.

238 isted in ejaculated human spermatozoa, while centriolar TSKS diminished in mouse sperm, where centrio

239 , which are necessary to form the C. elegans centriolar tube, a scaffold in centriole formation [4, 5

240 flagellar assembly function of specialized, centriolar tubulin cofactor C domain-containing proteins