ライフサイエンスコーパス: ceramidase

1 erized functional null mutants of Drosophila ceramidase.

2 , to inhibit the hydrolytic activity of acid ceramidase.

3 o produce and purify recombinant, human acid ceramidase.

4 s who lack the degradative enzyme galactosyl ceramidase.

5 S. cerevisiae that also encodes an alkaline ceramidase.

6 fied a rat brain membrane-bound nonlysosomal ceramidase.

7 indicate that the purified enzyme is a novel ceramidase.

8 the plasma with anti-ceramide antibodies or ceramidase.

9 educed transcriptional up-regulation of acid ceramidase.

10 amily member IV (PAQR4) as a Golgi-localized ceramidase.

11 tly explain its original identification as a ceramidase.

12 s dependent on generation of sphingosine via ceramidase.

13 mportant signaling lipid, by the activity of ceramidases.

14 s were completely resistant to inhibitors of ceramidases.

15 e degradation due to increased expression of ceramidases.

16 D-e-C(18:1)-ceramide, a common substrate of ceramidases.

17 nerated from the hydrolysis of ceramides via ceramidases.

18 cordingly called acid, neutral, and alkaline ceramidases.

19 transcriptomic analysis identifies alkaline ceramidase 1 (ACER1), a key enzyme in sphingolipid metab

20 +(o) markedly upregulates the human alkaline ceramidase 1 (haCER1) in HEKs; and its upregulation medi

21 CER1 is the human ortholog of mouse alkaline ceramidase 1 that we previously identified.

22 These data indicate that ASAH1 (acid ceramidase 1) and GBA2 (glucocerebrosidase 2) enzymes th

23 Herein we report that alkaline ceramidase 2 (ACER2) plays a key role in sustaining the

24 Human alkaline ceramidase 2 (ACER2) plays an important role in cellular

25 Here we demonstrate that the human alkaline ceramidase 2 (ACER2), a Golgi enzyme, regulates beta1 ma

26 up-regulated the expression of the alkaline ceramidase 2 (ACER2), and the ACER2 up-regulation decrea

27 The mouse alkaline ceramidase 2 gene (Acer2) is highly expressed in the pla

28 We have purified a membrane bound ceramidase 22,300-fold to apparent homogeneity.

29 te that our previously cloned human alkaline ceramidase 3 (ACER3) specifically controls the hydrolysi

30 study investigates the function of alkaline ceramidase 3 (ACER3)-catalyzed hydrolysis of unsaturated

31 In this study, we identified acid ceramidase (A-CDase) as a general transcription target o

32 The slab gene encodes ceramidase, a central enzyme in sphingolipid metabolism

33 Neutral ceramidase, a key enzyme of sphingolipid metabolism, hyd

34 The expression of acid ceramidase (AC) - a cysteine amidase that hydrolyses the

35 Acid sphingomyelinase (Asm) and acid ceramidase (Ac) are parts of the sphingolipid metabolism

36 whether physiological hypoxia increased acid ceramidase (AC) expression; and (f) the effect of the AC

37 e present study, smooth muscle-specific acid ceramidase (Ac) gene knockout mice (Asah1(fl/fl)/SM(Cre)

38 Lysosomal acid ceramidase (Ac) has been shown to be critical for cerami

39 Acid ceramidase (AC) hydrolyzes ceramides into sphingoid base

40 Acid ceramidase (AC) hydrolyzes ceramides, which are central

41 ulating ulcerative colitis, the role of acid ceramidase (AC) in intestinal inflammation is yet to be

42 c individuals (73%) exhibited increased acid ceramidase (AC) in their serum, measured by Western blot

43 Acid ceramidase (AC) is a cysteine hydrolase that plays a cru

44 Acid ceramidase (AC) is a key regulatory enzyme involved in c

45 Acid ceramidase (AC) is a lysosomal cysteine amidase that con

46 Human acid ceramidase (AC) is a lysosomal cysteine amidase, which h

47 Acid ceramidase (AC) is a lysosomal cysteine hydrolase that c

48 Acid ceramidase (AC) is a lysosomal hydrolase encoded by the

49 Acid ceramidase (AC) is an intracellular cysteine amidase tha

50 Acid ceramidase (Ac) is part of the sphingolipid metabolism a

51 Acid ceramidase (AC) is the lysosomal enzyme that degrades ce

52 We hypothesized that Acid Ceramidase (AC) overexpression would counteract the nega

53 utoproteolytic cleavage of the inactive acid ceramidase (AC) precursor into the active heterodimer ex

54 In addition, the acid ceramidase (AC) was also upregulated by Ca2+(o); and its

55 strate that ceramide-deacylating enzyme acid ceramidase (AC) was preferentially upregulated in irradi

56 yotubes that constitutively overexpress acid ceramidase (AC), an enzyme that catalyzes the lysosomal

57 evealed that desipramine down-regulated acid ceramidase (AC), but not sphingosine kinase, at the prot

58 The cysteine amidase, acid ceramidase (AC), hydrolyzes these substances into sphing

59 that the ceramide-metabolizing enzyme, acid ceramidase (AC), is expressed in human cumulus cells and

60 e gene (Asah1) encoding one ceramidase, acid ceramidase (AC), lead to the lysosomal storage disorder

61 inant-negative (DN)-TGFB, DN-Wnt8a, and acid ceramidase (AC)-to induce CM-like cells.

62 A and genomic sequences encoding murine acid ceramidase (AC; E.C. 3.5.1.23) have been isolated and ch

63 Herein we report the mechanism of human acid ceramidase (AC; N-acylsphingosine deacylase) cleavage an

64 the ASAH1 gene, resulting in deficient acid ceramidase (ACDase) activity.

65 l storage disorders caused by deficient acid ceramidase (ACDase) activity.

66 Interestingly, cells deficient in acid ceramidase (aCDase) also exhibited defects in CCL5 induc

67 Both acidic ceramidase (aCDase) and neutral ceramidase (nCDase) acti

68 In MVBs, acid ceramidase (aCDase) converts ceramide into sphingosine a

69 previously demonstrated that inhibiting acid ceramidase (aCDase) increases ceramide in HSCs to amelio

70 he deacylation of galactosylceramide by acid ceramidase (ACDase).

71 ation of ceramide through inhibition of acid ceramidase (aCDase).

72 dentified: 1 acid, 1 neutral, and 3 alkaline ceramidases (ACER1, ACER2, and ACER3).

73 t mutations in the gene (Asah1) encoding one ceramidase, acid ceramidase (AC), lead to the lysosomal

74 rotective action and investigate the role of ceramidase activation in adiponectin anti-inflammatory s

75 2C11 by IL-1beta in rat hepatocytes and that ceramidase activation provides a "switch" that determine

76 Acid ceramidase activities in skin fibroblasts and EBV-transf

77 IL-1beta increases both acid and neutral ceramidase activities, which appear to be regulated by t

78 n reduces) the basal and IL-1beta-stimulated ceramidase activities.

79 g the pcDNA3.1/His-ceramidase construct, and ceramidase activity (at pH 9.5) increased by 50- and 12-

80 void of any ceramidase activity restored the ceramidase activity and caused an increase in the hydrol

81 have demonstrated that BWA does not exhibit ceramidase activity and that bwa null mutants display no

82 ected, providing an accurate measure of acid ceramidase activity as low as 0.1 pmol/mg protein/h.

83 show that adiponectin potently stimulates a ceramidase activity associated with its two receptors, A

84 Not only alkaline ceramidase activity but also the generation of SPH and S

85 s revealed rapid internalization of the acid ceramidase activity from the hamster cell media but not

86 otein that is responsible for all measurable ceramidase activity in Drosophila.

87 dy reports a new assay method to detect acid ceramidase activity in vitro using Bodipy or lissamine r

88 Instead, D-e-MAPP inhibited alkaline ceramidase activity in vitro with an IC50 of 1-5 microM.

89 Here, we demonstrate that only alkaline ceramidase activity is expressed in erythrocytes and tha

90 Alkaline ceramidase activity is highly expressed in mouse erythro

91 Ceramidase activity is impaired in cells lacking both ad

92 ctively, these results suggest that alkaline ceramidase activity is important for the generation of S

93 The ceramidase activity is low, however, and further studies

94 eful wherever the in vitro detection of acid ceramidase activity is of importance.

95 ocytes have alkaline but not acid or neutral ceramidase activity on D-e-C(18:1)-ceramide, a common su

96 that the yeast gene YPC1 encodes an alkaline ceramidase activity responsible for the breakdown of dih

97 ssion in a yeast mutant strain devoid of any ceramidase activity restored the ceramidase activity and

98 A deficiency of acid ceramidase activity results in the lipid storage disorde

99 m by mediating, at least in part, a cellular ceramidase activity that catalyses the hydrolysis of cer

100 o receptors, AdipoR1 and AdipoR2, which have ceramidase activity that increases upon adiponectin bind

101 show that ADIPOR2 possesses intrinsic basal ceramidase activity that is enhanced by adiponectin.

102 of aPHC in mammalian cells elevated in vitro ceramidase activity toward N-4-nitrobenz-2-oxa-1,3-diazo

103 YPC1 ceramidase activity was confirmed by in vitro studies us

104 , perinuclear distribution, although no acid ceramidase activity was detected in the transfected cell

105 asing ceramide production by inhibiting acid ceramidase activity was sufficient to upregulate catheps

106 NC06 inhibits ceramidase activity with an IC(50) of 10.16-25.91 uM for

107 rements resulted (suggesting high endogenous ceramidase activity).

108 ls (Deltaypc1Deltaydc1) that lack endogenous ceramidase activity, and microsomes from ACER2-expressio

109 nd highly sensitive method to determine acid ceramidase activity, and that it could be useful whereve

110 receptors mediate their effects via a novel ceramidase activity, generating sphingoid base as a seco

111 ADIPOR1 also possesses intrinsic ceramidase activity, so we suspect that the two distinct

112 mouse kidney extracts as the source of acid ceramidase activity, this new method was compared with a

113 sease patient to the same extent as the acid ceramidase activity.

114 Paradoxically, cardiolipin stimulated ceramidase activity.

115 pe-independent and correlated with increased ceramidase activity.

116 of membranes by activating contiguous acidic ceramidase activity.

117 romoter, ultimately culminating in increased ceramidase activity.

118 and that bwa null mutants display no loss of ceramidase activity.

119 e endoplasmic reticulum although it retained ceramidase activity.

120 Treatment with recombinant acid ceramidase ameliorates the two pivotal features of cysti

121 e is remarkably homologous to human glucosyl ceramidase, an enzyme involved in the ceramide signallin

122 entrations (that induce AGP) do not activate ceramidase and allow Cer accumulation.

123 Manipulating levels of ceramidase and altering these lipids in spin mutants all

124 vide important new information on human acid ceramidase and further document its central role in sphi

125 with UC2288 prevented the induction of acid ceramidase and inhibited both the formation of PGCC from

126 ng a locus containing Acer2, which encodes a ceramidase and is specifically expressed in the collecti

127 suggest that p21 functions upstream of acid ceramidase and plays an important role in polyploidizati

128 ramide synthase, sphingomyelin synthase, and ceramidase and small interfering RNA knockdown of human

129 ALDI-IMS in combination with novel on-tissue ceramidase and sphingomyelinase enzyme digestions makes

130 ed to investigate function and expression of ceramidase and sphingomyelinase.

131 se A2 (PLA2) activation, markedly suppressed ceramidase and stimulated SMase activity.

132 mide preceded transient activation of acidic ceramidase and subsequent production of sphingosine, fol

133 effect on transcriptional activation of acid ceramidase and that CerS2 slightly but significantly dec

134 These studies provide new insights into acid ceramidase and the related lipid hydrolase, acid sphingo

135 r determining the activity and inhibition of ceramidases and may be adapted for quantifying sphingomy

136 iency: Ormdl1, sphingosine kinase-2, neutral ceramidase, and ceramide synthase-5.

137 liver mitochondria have ceramidase, reverse ceramidase, and thioesterase activities.

138 precipitated with acid ceramidase using anti-ceramidase antibodies.

139 f a cDNA encoding for a novel human alkaline ceramidase (aPHC) that hydrolyzes phytoceramide selectiv

140 Ceramidases are enzymes involved in regulating cellular

141 Therefore, ceramidases are key enzymes in the regulation of the cel

142 -transferase [SPT]) and ceramide hydrolysis (ceramidase) are elevated in obese adipose tissues.

143 de degradation to increase ceramide via acid ceramidase (ARN082) for proof of principle.

144 alysis of sequences homologous to human acid ceramidase (ASAH) revealed a 1233-bp cDNA (previously de

145 Acid ceramidase (ASAH1) plays a pivotal role in regulating th

146 alysis revealed that only expression of acid ceramidase (ASAH1) was increased.

147 Moreover, suppression of expression of acid ceramidase (ASAH1), an enzyme that produces SPH, increas

148 rmation depends on the lysosomal enzyme acid ceramidase (ASAH1).

149 dependence is demonstrated both by in vitro ceramidase assays and in intact hepatocytes using a fluo

150 eport detailed characterization of this acid ceramidase-associated "reverse activity" and provide evi

151 ine ratio increments), suggesting a possible ceramidase block.

152 Blocking acid ceramidase but not sphingosine kinase activity in alveol

153 these discrepancies are due to activation of ceramidase by the low concentrations of IL-1beta ( appro

154 Ceramidases catalyze the conversion of ceramide to sphin

155 rane bSMase-induced ceramide using bacterial ceramidase caused ERM hyperphosphorylation and formation

156 ngosine and sphingosine 1-phosphate (S1P) by ceramidase (CDase) and sphingosine kinase.

157 regulation of IRF8 prevented changes of acid ceramidase, ceramide, and sphingosine in CF epithelial c

158 These results suggest that the acid ceramidase/ceramide signaling pathway controls EV releas

159 EK 293 and MCF7 cells using the pcDNA3.1/His-ceramidase construct, and ceramidase activity (at pH 9.5

160 neration in arrestin mutant flies expressing ceramidase correlated with a decrease in ceramide levels

161 sembly of ceramide channels, as initiated by ceramidase, could be accelerated by the direct interacti

162 Ceramidases deacylate ceramides, important intermediates

163 exogenous sphingomyelinase or inhibition of ceramidase decreased SRE-mediated gene expression.

164 autosomal recessive disorder caused by acid ceramidase deficiency that usually presents as early-ons

165 limits of detection for sphingosine-NDA and ceramidase-derived sphingosine-NDA were 9.6 and 12.3 fmo

166 The activity of the purified ceramidase did not require cations, and it was inhibited

167 YPC1p, aPHC exhibited no reverse activity of ceramidase either in vitro or in cells.

168 Acid ceramidase, expressed in a recombinant SV, decreased int

169 Although ceramidase expression aids the removal of internalized r

170 Ceramidase expression facilitates the endocytic turnover

171 Therefore, the phenotypic consequence of ceramidase expression in photoreceptors is caused by fac

172 F8) and a concomitant downregulation of acid ceramidase expression with CF and an increase of ceramid

173 beta1-integrins downregulate acid ceramidase expression, resulting in further accumulation

174 Here, we show that expression of ceramidase facilitates the dissolution of incompletely f

175 ha) and 40 (beta)-kDa subunits as human acid ceramidase from natural sources, had an acidic pH optimu

176 We have previously purified a membrane-bound ceramidase from rat brain and recently cloned the human

177 These results indicate that SLAB ceramidase function controls presynaptic terminal sphing

178 We show that secreted ceramidase functions in a cell-nonautonomous manner to m

179 ts showed that the green fluorescent protein-ceramidase fusion protein presented a mitochondrial loca

180 Finally, a green fluorescent protein-ceramidase fusion protein was constructed to investigate

181 Galactosyl-ceramidase (GALC) is a ubiquitous lysosomal enzyme cruci

182 lb(cre) (liver-specific deletion of the acid ceramidase gene Asah1) mice.

183 Instead, the neutral ceramidase gene CDase encodes the protein that is respon

184 hat: (i) the structure of the murine neutral ceramidase gene is virtually identical to that of the hu

185 y functions to repress the expression of the ceramidase genes YDC1 and YPC1, thereby revealing, for t

186 Here we identify a novel human ceramidase (haCER2) that regulates the levels of both sp

187 inhibitor D-e-MAPP, suggesting that alkaline ceramidases have a role in the generation of SPH and S1P

188 Five human ceramidases have been identified: 1 acid, 1 neutral, and

189 We show that these two ceramidases have different substrate specificity, such t

190 nwashing (Bwa) is the only putative alkaline ceramidase homologue present in Drosophila.

191 The unchanged levels of galactosyl ceramidase, i.e., the enzyme lacking in Krabbe disease,

192 n cells, and it was a poor inhibitor of acid ceramidase (IC50>500 microM).

193 n mediate transcriptional activation of acid ceramidase in a JNK-dependent manner that is independent

194 ve demonstrated a downregulation of the acid ceramidase in CF epithelial cells resulting in an increa

195 Role of neutral ceramidase in colon cancer.

196 nects to a marked downregulation of the acid ceramidase in human and murine CF epithelial cells.

197 everal genes encode for variants of alkaline ceramidase in mammals.

198 Here, we find that deletion of neutral ceramidase in multiple breast cancer models in female mi

199 Loss of acid ceramidase in myeloid cells suppresses intestinal neutro

200 Overexpression of acid ceramidase in normal human skin fibroblasts also led to

201 Our results implicate acid ceramidase in the pathogenesis of GBA1-associated PD, su

202 ll-nonautonomous function, overexpression of ceramidase in tissues distant from photoreceptors suppre

203 e-MAPP functions as an inhibitor of alkaline ceramidase in vitro and in cells resulting in elevation

204 Deletion of myeloid neutral ceramidase in vivo and in vitro induces exhaustion in tu

205 These studies point to an important role for ceramidases in the regulation of endogenous levels of ce

206 Ceramidase inhibition increases the ceramide content of

207 tivation/C20:4 generation; (2) C20:4-induced ceramidase inhibition, coupled with SMase stimulation, m

208 Treatment with the ceramidase inhibitor ceranib-2 induced EBV lytic replica

209 S1P increases were inhibited by the alkaline ceramidase inhibitor D-e-MAPP, suggesting that alkaline

210 strongly by the incubation of cells with the ceramidase inhibitor D-erythro-2-tetradecanoylamino-1-ph

211 TNF-alpha and IL-1beta cross-signaling, the ceramidase inhibitor N-oleoyl ethanolamine (1 microM) or

212 is effect was mimicked by treatment with the ceramidase inhibitor N-oleoyl ethanolamine.

213 -2-(N-myristoylamino)-1-phenyl-1-propanol, a ceramidase inhibitor, and TNFalpha, a homologue of adipo

214 Acid ceramidase inhibitor, ARN082, elevated cellular ceramide

215 Similarly, exogenous ceramide or the ceramidase inhibitor, B13, induced CD1d gene expression

216 tment of nude mice with B13, the most potent ceramidase inhibitor, completely prevented tumor growth

217 Application of ceramide analogues and ceramidase inhibitors induced rapid cell death through a

218 ults indicate that the Asah2-encoded neutral ceramidase is a key enzyme for the catabolism of dietary

219 Neutral ceramidase is a key regulator of ceramide, the central i

220 These results demonstrate that this novel ceramidase is a mitochondrial enzyme, and they suggest t

221 monstrated that the sphingolipid enzyme acid ceramidase is dispensable for polyploidization upon ther

222 The Asah2-encoded neutral ceramidase is highly expressed in the small intestine al

223 Furthermore, our results show that secreted ceramidase is internalized and localizes to endosomes.

224 Acid ceramidase is one of the key enzymes that regulate the m

225 Mechanistically, myeloid neutral ceramidase is required for the generation of lipid dropl

226 ngosine, a product of ceramide hydrolysis by ceramidase, is capable of destabilizing ceramide channel

227 mide axis, which may be countered by neutral ceramidase, is suggested to limit cell motility and meta

228 show that, although BWA is unlikely to be a ceramidase, it is a regulator of sphingolipid flux in Dr

229 Fe did not directly affect HK-2 ceramidase levels.

230 renamed human ASAHL since it is a human acid ceramidase-like sequence), chromosomal location, primer

231 d characterization of a novel mouse alkaline ceramidase (maCER1) with a highly restricted substrate s

232 Similar to other alkaline ceramidases, maCER1 had an alkaline pH optimum of 8.0, a

233 he brush border, suggesting that the neutral ceramidase may be involved in a pathway for the digestio

234 phages have high constitutive levels of acid ceramidase mRNA, protein, and activity.

235 ogues of ceramide on rat brain mitochondrial ceramidase (mt-CDase) were investigated.

236 Acid ceramidase (murine gene code: Asah1) (50 kDa) belongs to

237 In this study, we report that neutral ceramidase (N-acylsphingosine amidohydrolase [ASAH2]) is

238 Acid ceramidase (N-acylsphingosine amidohydrolase) is the lys

239 solated and characterized the murine neutral ceramidase (N-CDase) gene, mapped its chromosomal locati

240 analogs (C2- or C6-ceramide) or inhibitor of ceramidase (N-oleoyl ethanolamine) led to a time- and do

241 sphingosine production using an inhibitor of ceramidase, n-oleoylethanolamine, completely abrogated t

242 Both acidic ceramidase (aCDase) and neutral ceramidase (nCDase) activities declined after L- and H-U

243 adiponectin significantly increased neutral ceramidase (nCDase) activity (3.7-fold; P<0.01).

244 itochondria due to the activation of neutral ceramidase (NCDase) and the reduced activity of sphingos

245 Neutral ceramidase (nCDase), a key enzyme in sphingolipid metabo

246 Neutral ceramidase (NcDase), which is highly expressed in the in

247 was markedly decreased in liver from neutral ceramidase (NCDase)-deficient mice.

248 ated analogues of ceramide and inhibitors of ceramidases offer a promising therapeutic strategy with

249 cts of treatment with recombinant human acid ceramidase on inflammation and infection.Methods: Sphing

250 cts of treatment with recombinant human acid ceramidase on sphingolipid profile and inflammatory medi

251 olino-1-propanol (PDMP), which inhibits acid ceramidase or glucosylceramide synthase and then increas

252 an myocardial function; and c) inhibition of ceramidase or nitric oxide synthase attenuates myocardia

253 tion, coupled with an on-tissue digestion by ceramidase or sphingomyelinase.

254 olic conversion, with specific inhibitors of ceramidase or sphingosine kinase, attenuated the expecte

255 cted protein product belongs to the alkaline ceramidase, PAQR receptor, Per1, SID-1, and TMEM8 (CREST

256 In the absence of ceramidase, photoreceptors degenerate in a light-depende

257 I tract raising the possibility that neutral ceramidase plays a detoxifying role against inadvertent

258 py stress promotes polyploidy, and that acid ceramidase plays a role in depolyploidization.

259 Using an acid ceramidase promoter driven luciferase reporter plasmid,

260 eramide antibodies or degradation by neutral ceramidase protected against infection with SARS-CoV-2.

261 veolar macrophages, because there was little ceramidase protein or activity (or sphingosine) in monoc

262 ction of anti-ceramide antibodies or neutral ceramidase rapidly abrogated stress-induced MDD, and int

263 uced inflammatory response via Cav1-mediated ceramidase recruitment and activation in an AdipoR1-depe

264 surements to study the role of IRF8 for acid ceramidase regulation.

265 findings establish PAQR4 as Golgi-localized ceramidase required for cellular growth in breast cancer

266 Targeted expression of Drosophila neutral ceramidase rescued retinal degeneration in arrestin and

267 h N-oleoylethanolamine (OE), an inhibitor of ceramidase, results in resistance to DNA damage-induced

268 that highly purified liver mitochondria have ceramidase, reverse ceramidase, and thioesterase activit

269 Sphingosine is generated by the action of ceramidase(s) on ceramide, and alveolar macrophages have

270 golipid metabolism by inducing expression of ceramidase, sphingosine kinases 1/2, and S1P receptors.

271 Moreover, knockdown of neutral ceramidase suppressed the formation of lamellipodia conc

272 Transgenic expression of ceramidase suppresses retinal degeneration in Drosophila

273 uced apoptosis in various organs may involve ceramidases that facilitate the degradation of ceramide.

274 Inhibition of acid ceramidase, the lysosomal enzyme that deacylates GluCer

275 Ceramidase, the only enzyme known to hydrolyze proapopto

276 hytoceramidase, the first mammalian alkaline ceramidase to be identified as being specific for the hy

277 hod utilizes purified human recombinant acid ceramidase to completely hydrolyze ceramide to sphingosi

278 Ceramide can be metabolized by ceramidase to sphingosine (Sph), and Sph to sphingosine

279 The contribution of neutral ceramidase to the reprogramming of the tumor microenviro

280 Treatment with recombinant human acid ceramidase, to decrease ceramide, reduced both inflammat

281 levels of sphingosine, which is generated by ceramidases upon hydrolysis of ceramide.

282 activity could be co-precipitated with acid ceramidase using anti-ceramidase antibodies.

283 R deficiency causes a downregulation of acid ceramidase via upregulation of IRF8, which is a central

284 The increase in acid ceramidase was confirmed by expression and activity anal

285 ed sphingolipid pathways and found that acid ceramidase was constitutively overexpressed in leukemic

286 Human acid ceramidase was overexpressed in Chinese hamster ovary ce

287 The high levels of acid ceramidase were specific to alveolar macrophages, becaus

288 interfering RNA knockdown of human alkaline ceramidase, which all increase endogenous ceramide level

289 induction of p21 and that knockdown of acid ceramidase, which hydrolyzes ceramide, does not interfer

290 ces cerevisiae gene YPC1 encodes an alkaline ceramidase with a dual activity, catalyzing both hydroly

291 data suggest that maCER1 is a novel alkaline ceramidase with a stringent substrate specificity and th

292 ore the physiological functions of a neutral ceramidase with diverse cellular locations, we disrupted

293 ly hydrolyzes phytoceramide, whereas the new ceramidase YDC1p hydrolyzes dihydroceramide preferential

294 In mammals, degradation of ceramide by ceramidase yields sphingosine, which is phosphorylated b

295 d on sequence homology to the yeast alkaline ceramidases YPC1p, we report the identification and clon

296 d 32% identity to the Saccharomyces alkaline ceramidases (YPC1p and YDC1p) and the human alkaline phy