ライフサイエンスコーパス: choline kinase

1 found N-terminal to the catalytic domain of choline kinase.

2 transient increase in the phosphorylation of choline kinase.

3 the predicted amino acid level with the rat choline kinase.

4 efforts selectively targeting P. falciparum choline kinase.

5 esidues are located at the catalytic core of choline kinase.

6 e the mechanism of catalysis by this enzyme, choline kinase A-2 from Caenorhabditis elegans was analy

7 e high degree of structural similarity among choline kinase A-2, aminoglycoside phosphotransferases,

8 ata on the homologous Caenorhabditis elegans choline kinase, a model of the ternary ADP.phosphocholin

9 phosphocholine levels (measured by MRS) and choline kinase activities (r2 = 0.95, P = 0.0008) follow

10 s encoded all of the ethanolamine kinase and choline kinase activities in S. cerevisiae.

11 Phosphatidylinositol synthase and choline kinase activities were not affected by respirato

12 so commonly increased both p70 S6 kinase and choline kinase activities.

13 ocholine levels were found to correlate with choline kinase activities.

14 ADP inhibited choline kinase activity (IC50 = 0.32 mM) in a positive c

15 ells was primarily attributable to increased choline kinase activity and increased catabolism mediate

16 The regulation of choline kinase activity by ATP and ADP may be physiologi

17 In addition, enzymatic assays of choline kinase activity in cells were done.

18 EKI1 has negligible choline kinase activity in vitro and does not influence

19 inc-depleted cells translated into increased choline kinase activity in vitro and in vivo, and an inc

20 the protein kinase A-mediated stimulation in choline kinase activity involved an increase in the appa

21 Maximum choline kinase activity was dependent on Mg2+ ions (10 m

22 ither reaction, an unexpected enhancement of choline kinase activity was observed specifically with t

23 se A resulted in a stimulation (1.9-fold) in choline kinase activity whereas alkaline phosphatase tre

24 The EKI1 gene product also exhibited choline kinase activity.

25 choline kinase resulted in a 60% decrease in choline kinase activity.

26 rylation was accompanied by a stimulation in choline kinase activity.

27 d enhanced the stimulatory effect of zinc on choline kinase activity.

28 ne kinase, and it was also poor inhibitor of choline kinase activity.

29 effectively inhibited only the GmCK2-encoded choline kinase activity.

30 herichia coli demonstrated that each encodes choline kinase activity.

31 was accompanied by a 1.6-fold stimulation of choline kinase activity.

32 cki1Delta eki1Delta double mutant that lacks choline kinase activity.

33 n, but rather due to increased expression of choline kinase alpha (CHKA) and an activated deacylation

34 Choline kinase alpha (CHKA) is a central mediator of cel

35 ly increasing expression of the compensatory choline kinase alpha (Chka) isoform, preventing muscle c

36 In addition, we examined the ability of choline kinase alpha (Chka), a gene paralog of Chkb, to

37 new mechanism of EGFR-c-Src synergy through choline kinase alpha (CHKA).

38 ymes involved in lipid metabolism, including choline kinase alpha (ChoK(alpha)), fatty acid synthase

39 Choline kinase alpha (ChoKalpha) is an enzyme involved i

40 Human choline kinase alpha (CKalpha) is a validated drug targe

41 ynthesis and correlated with an induction of choline kinase alpha expression.

42 ether, these data offer strong evidence that choline kinase alpha has a heretofore underappreciated r

43 Here, we show that choline kinase alpha interacts with the SH3 domain of c-

44 Choline kinase alpha is a 457-residue protein that catal

45 Furthermore, pharmacological inhibition of choline kinase alpha, an enzyme that catalyzes phosphoch

46 for a non-catalytic protein-binding role for choline kinase alpha.

47 -3-phosphate acyltransferase GPAM along with choline kinase-alpha (CHKA), the enzymes that catabolize

48 Choline kinase-alpha (ChoKalpha) is a metabolic enzyme t

49 Choline kinase-alpha activity is required for the downst

50 7) that inhibited purified recombinant human choline kinase-alpha activity, reduced the steady-state

51 Together, these results further validate choline kinase-alpha as a molecular target for the devel

52 Choline kinase-alpha expression and activity are increas

53 ilitate the identification of new classes of choline kinase-alpha inhibitors.

54 r small molecules that may interact with the choline kinase-alpha substrate binding domain, we identi

55 The product of choline kinase-alpha, phosphocholine, serves as an essen

56 providing a method to observe the action of choline kinase, an important target for novel cancer the

57 The enzymatic activities of choline kinase and choline-phosphate cytidylyltransferas

58 The activities of both choline kinase and choline-phosphate cytidylyltransferas

59 esis pathway combining conserved prokaryotic choline kinase and CTP:phosphocholine cytidylyltransfera

60 dicted to encode a fusion protein containing choline kinase and CTP:phosphocholine cytidylyltransfera

61 A gene was cloned, and recombinant LicCA had choline kinase and CTP:phosphocholine cytidylyltransfera

62 ng a cki1Delta eki1Delta mutant defective in choline kinase and ethanolamine kinase, we examined the

63 This gene encodes choline kinase and is also involved in phase variation o

64 s were consistent with the overexpression of choline kinase and phospholipase C detected in the micro

65 Choline kinase and phospholipase C were significantly ov

66 ntered on stopping the catalytic activity of choline kinase and reducing the downstream metabolites i

67 lamine was a poor substrate for the purified choline kinase, and it was also poor inhibitor of cholin

68 ined in a protein kinase A sequence motif in choline kinase are target sites for protein kinase A.

69 Using choline kinase as a substrate, protein kinase C activity

70 f thermotolerance, and At1g74320 encodes for choline kinase (AtCK2) that catalyzes the first reaction

71 The CKI1-encoded choline kinase (ATP:choline phosphotransferase, EC 2.7.1

72 In the yeast Saccharomyces cerevisiae, choline kinase (ATP:choline phosphotransferase, EC 2.7.1

73 caused by loss of function mutations in the choline kinase beta (CHKB) gene which results in dysfunc

74 n is a 1.6-kb intragenic deletion within the choline kinase beta (Chkb) gene, resulting in a complete

75 ans, the CPT1B gene is closely linked to the choline kinase beta (CHKB) gene, which is transcribed fr

76 The CHKB gene encodes choline kinase beta, which catalyzes the first step in t

77 Inhibition of choline kinase by MN58b resulted in altered phospholipid

78 tation did not affect the phosphorylation of choline kinase by protein kinase A, the S30A (protein ki

79 The Saccharomyces cerevisiae CKI1-encoded choline kinase catalyzes the committed step in phosphati

80 Choline kinase catalyzes the committed step in the synth

81 t Saccharomyces cerevisiae, the CKI1-encoded choline kinase catalyzes the committed step in the synth

82 Choline kinase catalyzes the phosphorylation of choline

83 t glucose deprivation induces the binding of choline kinase (CHK) a2 to lipid droplets, which is sequ

84 CHKB is one of two mammalian choline kinase (CHK) enzymes (alpha and beta) that catal

85 ip between hypoxia, choline metabolites, and choline kinase (Chk) in a human prostate cancer model.

86 Effective silencing of choline kinase (chk), the enzyme that converts choline t

87 Choline kinase (Chk), the enzyme that converts choline t

88 Choline kinases (ChKs) could also be critical in the ear

89 oline (trimethyl-2-hydroxyethylammonium) and choline kinase (CK) activity in neoplasms have motivated

90 stoichiometry (0.44 mol of phosphate/mol of choline kinase) consistent with one phosphorylation site

91 The functional importance of RNAi-mediated choline kinase down-regulation on choline phospholipid m

92 choline, other potential substrates for the choline kinase enzyme include ethanolamine, monomethylet

93 eptide maps of the wild type and S30A mutant choline kinase enzymes phosphorylated by protein kinase

94 CHKB encodes one of two mammalian choline kinase enzymes that catalyze the first step in t

95 Choline kinase exhibited saturation kinetics with respec

96 Native choline kinase existed in oligomeric structures of dimer

97 t small interfering RNA (siRNA) silencing of choline kinase expression in transformed HeLa cells comp

98 se results strongly support the targeting of choline kinase in breast cancer cells with RNAi and show

99 essed normally, but the specific activity of choline kinase in cells expressing the S30A, S85A, and S

100 he purified enzyme and were used to identify choline kinase in insect cells and in S. cerevisiae.

101 Crystal structures of human choline kinase in its apo, ADP and phosphocholine-bound

102 erference (RNAi)-mediated down-regulation of choline kinase in nonmalignant and malignant human breas

103 e the existence of separate ethanolamine and choline kinases in mammals and show that ethanolamine ki

104 naling and partially rescues HeLa cells from choline kinase inhibition.

105 inase substrate choline (250 microM) and the choline kinase inhibitor hemicholinium-3 (2 mM) enhanced

106 The choline kinase inhibitor hemicholinium-3 inhibits approx

107 veals a mode of action for two P. falciparum choline kinase inhibitors both in vitro and in vivo.

108 y challenges in the development of drug-like choline kinase inhibitors.

109 ning tumor response following treatment with choline kinase inhibitors.

110 In this work we examined the hypothesis that choline kinase is also phosphorylated by protein kinase

111 These results show that choline kinase is encoded by a small, multigene family i

112 Choline kinase is overexpressed in breast cancer cells a

113 The Saccharomyces cerevisiae CKI-encoded choline kinase is phosphorylated on a serine residue and

114 Choline kinase is upregulated in prostate cancer, result

115 Both soybean choline kinase isoforms demonstrated negligible ethanola

116 ndent and dependent on the concentrations of choline kinase (K(m) = 27 microg/ml) and ATP (K(m) = 15

117 lted in the derepression of the CKI1-encoded choline kinase (Kennedy pathway enzyme) but decreased th

118 he concentrations of ATP (Km 2.1 microM) and choline kinase (Km 0.12 microM).

119 olamine kinase activity of the P. falciparum choline kinase, leading to a severe decrease in the phos

120 A third unique choline kinase-like cDNA, GmCK3, was also identified but

121 nd yeast choline kinases was used to isolate choline kinase-like cDNAs from soybean (Glycine max L.).

122 e N-terminal amino acid sequence of purified choline kinase matched perfectly with the deduced sequen

123 f, and Asp-301 and Glu-303, in the signature choline kinase motif.

124 The full-length LicA polypeptide resembles choline kinases of eucaryotes, suggesting that the pathw

125 In vitro, protein kinase A phosphorylated choline kinase on a serine residue with a stoichiometry

126 orted the conclusion that phosphorylation of choline kinase on Ser(30) and Ser(85) by protein kinase

127 owth factor treatment, but the activities of choline kinase or choline-phosphate cytidylyltransferase

128 control, but it did not alter activities of choline kinase or cholinephosphotransferase.

129 Choline kinase phosphorylates choline to phosphocholine

130 In support of the role of the choline kinase product phosphocholine in the mediation o

131 er(30) and Ser(85), respectively, within the choline kinase protein were substrates for protein kinas

132 RNAi knockdown of choline kinase reduced proliferation, as detected by pro

133 e/ethanolamine-binding site of P. falciparum choline kinase, reflecting different types of inhibition

134 Choline kinase, responsible for the phosphorylation of c

135 ty whereas alkaline phosphatase treatment of choline kinase resulted in a 60% decrease in choline kin

136 A Ser25 to Ala (S25A) mutation in choline kinase resulted in a 60% decrease in protein kin

137 58b is a novel anticancer drug that inhibits choline kinase, resulting in inhibition of phosphocholin

138 olic action has been well studied because of choline kinase's link to cancer malignancy and poor pati

139 Streptococcus pneumoniae choline kinase (sChoK) has previously been proposed as a

140 Hemicholinium-3 (HC-3), an inhibitor of choline kinase, strongly inhibited UV-induced AP-1 activ

141 itogenic Ca2+ effects, cotreatments with the choline kinase substrate choline (250 microM) and the ch

142 The turnover number per choline kinase subunit was 153 s-1.

143 se) consistent with one phosphorylation site/choline kinase subunit.

144 A choline kinase synthetic peptide (SQRRHSLTRQ) containing

145 dylcholine (PtdCho), is initiated by a novel choline kinase (TgCK).

146 subunit molecular mass (73 kDa) of purified choline kinase was in good agreement with the predicted

147 ere were consistent with the conclusion that choline kinase was regulated by protein kinase A phospho

148 yed sequence homology to mammalian and yeast choline kinases was used to isolate choline kinase-like

149 MthK acts as a bifunctional choline kinase which can utilize ATP or the MthB demethy

150 to the Saccharomyces cerevisiae CKI1-encoded choline kinase, which also exhibits ethanolamine kinase

151 ares its regulatory region with heat-induced choline kinase, which has a possible role in heat signal

152 Phosphorylation of choline kinase with protein kinase A resulted in a stimu

153 Choline kinase with Ser(30) to Ala (S30A) and Ser(85) to