ライフサイエンスコーパス: circularization

1 tes cassette mobilization (i.e. excision and circularization).

2 hese factors inhibits Drosophila tRNA intron circularization.

3 own to be important, but not sufficient, for circularization.

4 5E and M5 inhibited primer translocation and circularization.

5 rand DNA synthesis: primer translocation and circularization.

6 irus (DHBV) makes a positive contribution to circularization.

7 DNA amplifications that accompany chromosome circularization.

8 site for the second template switch, termed circularization.

9 in both primer translocation/utilization and circularization.

10 location/utilization and a partial defect in circularization.

11 They were also invariably defective for circularization.

12 to maintain telomeres and prevent chromosome circularization.

13 ive for primer translocation/utilization and circularization.

14 for primer translocation and 5'r and 3'r for circularization.

15 the second template switch, a process called circularization.

16 g rapid loss of telomeric DNA and chromosome circularization.

17 rate of the protein increased slightly upon circularization.

18 telomeres by a unique pathway of chromosome circularization.

19 se of a DNA template allowed their efficient circularization.

20 DNA in an unbent configuration that resists circularization.

21 , priming of new strand synthesis and genome circularization.

22 ead ribozyme capable of self-cleavage and re-circularization.

23 ill-in DNA assembly, and Cre-lox in vivo DNA circularization.

24 contrast, gammaH2AX did not function in KSHV circularization.

25 ing open reading frames, RNA fusions and RNA circularization.

26 regulated by Quaking (QKI)-mediated splicing circularization.

27 indicate both NHEJ and HR contribute to KSHV circularization.

28 Therefore, the DDR mediates KSHV and HSV-1 circularization.

29 ong reads or Illumina short reads and genome circularization.

30 king current models insufficient to describe circularization.

31 er a systematic effect of exon length on RNA circularization.

32 y components in unusual ways and promote RNA circularization.

33 diates, cleavage to unit-length strands, and circularization.

34 ly a characteristic essential for plasmid re-circularization.

35 r selection of cloning events against vector circularization.

36 ss of the integrase that was responsible for circularization.

37 d telomere maintenance or through chromosome circularization.

38 pairing during both primer translocation and circularization.

39 d therefore require NHEJ during phage genome circularization.

40 ATM are required for efficient scAAV genome circularization.

41 or Omega, with concomitant failure of genome circularization.

42 1 and 3' end-associated KPAF4 to enable mRNA circularization.

43 by contributing to primer translocation and circularization.

44 hird body followed by tidally driven orbital circularization(2,3).

45 the merger, as basic physics would argue for circularization(6).

46 Circularization achieved with DNA ligase, followed by li

47 fication procedure, RCA-RCA (Restriction and Circularization-Aided Rolling Circle Amplification), whi

48 viral RNAs bypass this mechanism by blocking circularization, allowing efficient translation despite

49 This interaction may enable mRNA circularization, an apparently critical element of mitoc

50 e tail-to-head organization is the result of circularization and breakage of a GAPDH retrogene prior

51 Improvements in cargo design such as RNA circularization and data-driven untranslated region opti

52 rger amounts of pure full-length genomes for circularization and infectivity measurements.

53 es, and absence of both severely compromises circularization and latency.

54 metabolic stability of the sense strand upon circularization and off-target effects were eliminated.

55 Additionally, the circularization and possible fate of HSV genomes are reg

56 rclator, the first tool to automate assembly circularization and produce accurate linear representati

57 This protocol consists of the circularization and purification of linear double-strand

58 ing to oncogenic remodeling through chimeric circularization and reintegration of circular DNA into t

59 Sequence analysis confirmed chromosome circularization and revealed the insertion of adventitio

60 ull-length cDNA libraries, followed by their circularization and the sequencing of the junction fragm

61 o detectable DNA binding based on gel shift, circularization and theta(275) reduction assays.

62 lation undergo telomere deletion, chromosome circularization, and amplification of subtelomeric DNA.

63 at play, however, as not all repeats support circularization, and increasing the stability of the hai

64 s, deletion of additional DNA during plasmid circularization, and insertion of chromosomal DNA fragme

65 ic machinery of these molecules, the role of circularization, and the differences in the possible cir

66 This RNA self-circularization approach to RNA sequencing (RC-Seq) allo

67 template switches, primer translocation and circularization, are required during the synthesis of th

68 Circularization assays showed that HMG-1, but not HMG-I/

69 mina Nextera Mate Pair (NMP) protocol uses a circularization-based strategy that leaves behind 38-bp

70 We suggest that Cp mRNA circularization brings the cytosolic Cp 3'-UTR-binding f

71 s that the presence of these elements favors circularization, but with modest predictive power.

72 cted cells, possibly due to low-level genome circularization by a cellular enzyme.

73 and in vivo studies on splicing-mediated RNA circularization by demonstrating the intracellular produ

74 We combined both PEGylation and circularization by exploiting two distinct sortase enzym

75 providing repetitive sequences suitable for circularization by non-recA-dependent pathways following

76 II introns, and cRNAs developed via in-cell circularization by the ubiquitously expressed RtcB prote

77 Circularization can improve RNA persistence, yet simple

78 Protein circularization can significantly impact protein enginee

79 When activation occurred after DNA circularization, cohesion persisted.

80 When gene activation occurred before DNA circularization, cohesion was lost.

81 release into the host nucleus, resulting in circularization, concatemer formation, or chromosomal in

82 ain, which promoted PAB1 oligomerization and circularization, correspondingly accelerated CCR4 deaden

83 approximately 400 nucleotides [nt]) promote circularization cotranscriptionally, whereas pre-mRNAs c

84 lementary 10-23 variants were inactivated by circularization, creating deoxyribozymogens.

85 Circularization (crosslinking the N and C termini) of th

86 The circularization-dependent and orientation-specific scAAV

87 We have developed circularization-dependent and orientation-specific self-

88 ecific cellular proteins involved, the scAAV circularization-dependent vector was used as a reporter

89 Circularization did not lead to a significant thermodyna

90 mple ligation of genomic ends and shows that circularization does not occur by annealing of single-st

91 and biochemical studies reveal that backbone circularization does not prevent the adoption of the nat

92 Circularization does not require protein synthesis in th

93 in the binary, followed by orbital decay and circularization due to tidal dissipation in the stars.

94 template switches (primer translocation and circularization) during synthesis of plus-strand DNA to

95 template switches, primer translocation and circularization, during plus-strand DNA synthesis.

96 tiffer DNA than normal cytosine, with poorer circularization efficiencies and lower ability to form n

97 This circularization enhances the IRES activity of SHMT1 by f

98 d from differentiation-regulated alternative circularization events within the same gene, each contai

99 Gel electrophoresis and self-ligation circularization experiments revealed that the CG*C-II du

100 Special categories of splicing such as exon circularization, first and last intron processing, alter

101 enomes were examined for five mutants by RNA circularization followed by cDNA synthesis, amplificatio

102 Here we describe 'circularization for high-throughput analysis of nuclease

103 Here we describe circularization for in vitro reporting of cleavage effec

104 he off-target effects using a combination of circularization for in vitro reporting of cleavage effec

105 Notably, RNA circularization for mRNAs with viral 5' UTRs restores co

106 cing requires the essential elements of mRNA circularization, i.e., eukaryotic initiation factor 4G,

107 We show that for single-cell RNA-Seq circularization improves the recovery of accurate single

108 Deletion of taz1 did not suppress chromosome circularization in cells lacking Rad3/Rad26 and Tel1/Rad

109 circular RNA biogenesis that may account for circularization in genes that lack noticeable flanking i

110 rnal PAI segment was capable of excision and circularization in the donor, and is mobilized as a coin

111 ested by the routing of nuclear viral DNA to circularization in the form of 2-long terminal repeat (2

112 ction of eIF3, and supporting a role of mRNA circularization in the mechanisms governing mRNA transla

113 ose termini are capable of undergoing facile circularization in vitro.

114 reconstitution approach, we demonstrate that circularization inhibits de novo translation initiation

115 This can lead to circularization, integration, or the formation of extrac

116 Circularization involves transfer of the nascent 3' end

117 Circularization involves transfer of the nascent plus st

118 eviously demonstrated that selective genomic circularization is a robust in-solution approach for cap

119 The circularization is based on use of a vector that contain

120 However, how RNA circularization is connected to alternative splicing rem

121 These results suggest that mRNA circularization is crucial for initiating codon-usage-de

122 we propose that the process of excision and circularization is important in the emergence, pathogene

123 These results mean that the process of circularization is influenced by the earlier steps in DN

124 results suggest that productive rAAV genome circularization is mediated primarily by nonhomologous e

125 s suggest that intramolecular recombination (circularization) is far more efficient than intermolecul

126 on with small interfering RNAs targeting the circularization junction of circHIPK3 or elevated using

127 of a hospital Klebsiella pneumoniae strain, circularization junctions (CJs) were detected for six GI

128 Herein, circularization kinetics and affinity measurements with

129 estigated by a variety of methods, including circularization kinetics, apparent helical repeat determ

130 nd strand transferase and play a role in the circularization, linearization and possibly integration

131 RNA circularization may be a general replication mechanism f

132 escue the destabilized mutant indicates that circularization may be a useful tool in protein engineer

133 ocessing signal is required, suggesting that circularization may occur post-transcriptionally.

134 ng the efficiency of translation, transcript circularization may serve as an essential structural det

135 ization, and the differences in the possible circularization mechanisms.

136 eir abilities to facilitate ligase-catalyzed circularization of a linear 88 bp DNA molecule, and to r

137 eaction has been used for the intramolecular circularization of a single stranded oligonucleotide whi

138 The catalytic efficiency of the circularization of a single-stranded substrate was 5-fol

139 RNAs'), we identified CircPVT1, generated by circularization of an exon of the PVT1 gene, as a circul

140 one from either Tn916 or Tn5386 promoted the circularization of constructs from the three different t

141 laccase2 flanking introns support efficient circularization of diverse exons in Drosophila and human

142 Extrachromosomal circularization of DNA is an important genomic feature i

143 Circularization of each PLP molecule is dependent upon h

144 requires DDR, and inhibiting DDR impairs the circularization of ecDNA.

145 We find that circularization of exons is widespread and correlates wi

146 The foundation of this approach is the circularization of fragmented viral RNAs, which are then

147 to normal human cells was reported to induce circularization of genes and chromosomes, in bacteria, t

148 ial artificial chromosome (BAC) was based on circularization of head-to-tail concatemers of VAC DNA.

149 show that double repeats that are formed by circularization of infecting genomes are rapidly convert

150 ovalently closed circular DNA, was formed by circularization of linear DNA by nonhomologous recombina

151 erfect duplex DNA, nor is it able to mediate circularization of linear duplex DNA.

152 Our findings also suggest that spontaneous circularization of linear Streptomyces chromosomes may b

153 However, it is not clear whether circularization of lncRNA happens only in nucleus or it

154 rring atheroprotection, thereby showing that circularization of long non-coding RNAs may alter RNA fu

155 n with the sub-Neptune prevents the complete circularization of LP 791-18d's orbit, resulting in cont

156 sequences can restore pairing to improve the circularization of poor circularizers.

157 whether a lack of DNA-PKcs activity reduces circularization of rAAV genomes in SCID muscle and wheth

158 tudy also suggests the possible RBP-mediated circularization of RNA in the cytoplasm through back-spl

159 of relaxed circular DNA by the didomain and circularization of short DNA fragments (in the presence

160 r, these complexes no longer facilitated the circularization of short DNA molecules and had lost the

161 We conclude that circularization of SMC5/6 provided by the kleisin NSE4 i

162 constructs, called selectors, that guide the circularization of specific DNA target regions.

163 Circularization of T-DNA by the FLP/FRT site-specific re

164 ilar to KSHV, ATM and DNA-PKcs have roles in circularization of the alpha herpesvirus, herpes simplex

165 r a more sustainable agriculture system, the circularization of the crop industries is of the utmost

166 plasmids and RNAi screening, we reveal that circularization of the Drosophila laccase2 gene is regul

167 ase 1 specifically and efficiently catalyzes circularization of the genuine PSTVd monomeric linear re

168 These results suggest that circularization of the HSV genome may not occur early in

169 Circularization of the infecting Mu DNA does not require

170 ed shape changes are not fragmentation but a circularization of the inner mitochondrial membrane, whi

171 s in formation of internal organs, including circularization of the intestine and bifurcation of the

172 The model postulated two steps (i) circularization of the linear DNA molecule that had been

173 that are joined by random recombination upon circularization of the linear genome at entry into cells

174 Following circularization of the linear viral genome, DNA replicat

175 ysis toolkit that automates the assembly and circularization of the mitochondrial genomes of Kinetopl

176 ES-mediated translation of SHMT1 whereby the circularization of the mRNA typically provided by the eu

177 es which are said to promote translation via circularization of the mRNA, but in no case has this bee

178 Subsequent circularization of the oligonucleotide label facilitated

179 significantly greater amounts of bending and circularization of the one-base overhang undecamer duple

180 d telomere by homologous recombination; (ii) circularization of the plasmid by non-homologous end-to-

181 ve transposition of Tn916 and is followed by circularization of the transposon and its transfer to a

182 of genome configuration revealed that rapid circularization of the viral DNA occurred on entry, thou

183 integration, excision, and extrachromosomal circularization of these elements, and they have similar

184 an increase in the frequency of excision and circularization of Tn916 caused by expression of integra

185 In contrast, deletions, amplifications, and circularizations of a wild-type strain happened at heter

186 Deletions, amplifications, and circularizations of the linear 8.7-Mb chromosome occurre

187 ward the intracellular space, preventing its circularization or even assembly on a relatively flat me

188 behaviour of this molecule in assays such as circularization or gel electrophoresis can only be under

189 nd in the appropriate state to permit either circularization or integration of the viral DNA in vivo.

190 performance of SpyCatcher-SpyTag in nanodisc circularization paves the way for the use of cNDs in mem

191 Flow-cytometric and digestion circularization PCR analyses revealed that delta c-Rel B

192 In contrast, digestion-circularization PCR analysis and high-throughput sequenc

193 nation to S gamma1, as measured by digestion-circularization PCR, is dramatically reduced in STAT6-de

194 c DNA recombination as measured by digestion-circularization PCR.

195 ractions of three essential elements of mRNA circularization, poly(A), PABP, and eIF4G.

196 the level of DNA recombination by digestion-circularization polymerase chain reaction (DC-PCR).

197 ng the 3' elements, as measured by digestion circularization-polymerase chain reaction or by the expr

198 duced as a result of exon skipping, with its circularization possibly occurring without the involveme

199 s of the ncRNAs were determined using an RNA circularization procedure.

200 erminal repeat retrotransposon DNA through a circularization process and is therefore necessary for e

201 erminal redundancy and the efficiency of the circularization process in those variants.

202 ition of the terminal redundancy made to the circularization process.

203 ) are nearly superimposable, indicating that circularization produces no substantial change in the lo

204 ting disk extending at most to the so-called circularization radius, and that the disk precesses as a

205 r chromosomal double-strand breaks, and cDNA circularization removes the pro-apoptotic signal.

206 In contrast to the chromosomal circularization reported previously in Schizosaccharomyc

207 Circularization requires formation of long-range Alu-med

208 n) expression system for achieving rapid RNA circularization, resulting in RNA aptamers with high sta

209 In contrast, we find circularization results in a dramatic stabilization of a

210 at the processes of primer translocation and circularization share a common underlying mechanism.

211 at the processes of primer translocation and circularization share a mechanism during which 5E, M, an

212 Basic polymer theory predicts that circularization should lead to a net thermodynamic stabi

213 d to identify the P9 domain as important for circularization, showing that swapping sequences can res

214 The circularization site is processed by the tRNA splicing e

215 Here we describe a method, SNP linkage by circularization (SLiC), to identify linkage between CAG

216 ation), which utilizes an efficient DNA self-circularization step during library preparation.

217 We employ an intramolecular circularization step that increases the efficiency of li

218 alt-EJ) DNA repair process of the host for a circularization step to synthesize their second-strand D

219 roidae, the host enzyme catalyzing the final circularization step, has remained elusive.

220 Upon circularization, the torsion constant increased by a fac

221 Together with RNA barcode stabilization via circularization, these scalable single-cell quantitative

222 ear PLP with a target DNA sequence; (ii) PLP circularization through enzymatic ligation; and (iii) qR

223 lizes restriction digestion and whole genome circularization to generate genomic sequences amenable t

224 eccentricity oscillations and a future tidal circularization trajectory.

225 Although primer translocation and circularization use different donor and acceptor sequenc

226 r RNAs (cRNAs): cRNAs developed via in vitro circularization using group II introns, and cRNAs develo

227 s end-joining is not required for chromosome circularization was further supported by analysis of sur

228 For these variants, the subsequent circularization was inhibited.

229 its template was restored to 54 nucleotides, circularization was substantially restored.

230 tain specific cases, such as the kinetics of circularization, where the rate-limiting step is a high-

231 es from some Tn916-like elements can promote circularization with termini derived from heterologous t

232 f back-splicing events showed widespread RNA circularization, with the average tumor expressing 7,232