ライフサイエンスコーパス: cloned

1 and minor allergens have been identified and cloned.

2 e been mapped, no resistance genes have been cloned.

3 over the parental clone were FACS-sorted and cloned.

4 Here we report that WFhb1-1 has been cloned.

5 ength cDNA of the 14,538-nt viral genome was cloned.

6 Here we have cloned 113 human monoclonal antibodies (mAbs) specific f

7 We cloned 17 AAE genes, expressed them heterologously in Es

8 was sensitive enough to detect one copy of a cloned 23S-5S IGS fragment from "CandidatusRickettsia am

9 Cloned 30 years ago, it was characterized as a flavoenzy

10 We identified and cloned a family of three inositol hexakisphosphate kinas

11 In this work we cloned a full-length cDNA encoding an LPXRFa precursor i

12 To address this we have cloned a full-length cDNA encoding the P. xylostella RyR

13 In this study, we cloned a full-length sequence for the Gnih precursor of

14 We cloned a full-size Tg coding sequence from western clawe

15 We identified a L. edodes C-S lyase (Lecsl), cloned a gene of Csl encoded Lecsl and then combined mod

16 We then cloned a hpRNA targeting YFP under the regulation of the

17 We have cloned a malarial antigen-containing fusion protein, MBP

18 We have cloned a miR395 gene from rice (Oryza sativa) and studie

19 ignaling mutants in Arabidopsis thaliana, we cloned a mutant allele of a gene that encodes a protein

20 e trait locus (QTL) analysis, identified and cloned a novel cinnamyl alcohol dehydrogenase allele (Sb

21 Here, we have identified and cloned a pollen-expressed P. rhoeas threonine-aspartate-

22 In this study, we cloned a putative i-antigen gene from C. irritans, which

23 In this study, we cloned a representative set of mAbs elicited by a model

24 We cloned a set of P5betaR genes from angiosperm plant spec

25 so restored by the exogenous expression of a cloned A* gene.IMPORTANCE Double-stranded DNA viruses ty

26 Ectopic overexpression of the newly cloned AA-enriched variant, PIK3CD-S, in EA PCa cell lin

27 control CER3 transcript levels directly, we cloned additional genes identified in our suppressor scr

28 All cloned AHAs could restore immune effector functions to p

29 I-2 produced by recombinant strains carrying cloned AI synthase genes, increased survival of V. chole

30 ition, compounds that acted as agonists at a cloned alpha-like BiOctR also induced a hyperactivity re

31 In this study, we cloned an isopentenyl pyrophosphate isomerase (IPPI) cDN

32 We cloned and analysed selected upstream regulatory regions

33 In this study, we cloned and analyzed chromosome markers from cultivated p

34 One of the predicted lancelet BCOL proteins, cloned and analyzed for carotenoid cleavage activity in

35 ptide (Ntcp) from the respective species was cloned and analyzed for HBV and HDV receptor activity in

36 We cloned and analyzed the expression of the genes Emx1, Lh

37 Herein, we cloned and biochemically characterized cDNA encoding CBS

38 We previously cloned and characterised alternatively spliced variants

39 to this process, AGAP2 proximal promoter was cloned and characterised using reporter assays.

40 cal steps toward (iso)eugenol production, we cloned and characterized a caffeoyl-coenzyme A O-methylt

41 In the present study, we cloned and characterized a wheat (Triticum aestivum) aux

42 To test this hypothesis we first cloned and characterized ALOX15 orthologs of selected Ca

43 s study, a full-length cDNA encoding CPR was cloned and characterized from T. cinnabarinus (designate

44 protective humoral response against HBV, we cloned and characterized human antibodies specific to th

45 Here, we cloned and characterized Lavandula x intermedia cDNAs en

46 Here we cloned and characterized rare affinity-matured human NAN

47 Here we have cloned and characterized the iridoid synthase enzyme fro

48 l steps after p-coumaric acid production, we cloned and characterized three petunia transcripts with

49 Two decades later, it was cloned and characterized to be a transmembrane mucin, MU

50 it variation in sorghum have been previously cloned and characterized.

51 In both diseases, the autoantigens have been cloned and characterized; pemphigus antigens are desmogl

52 We cloned and expanded mouse elastin-specific CD4(+) T cell

53 se two receptor genes along with CsPYL1 were cloned and expressed in a heterologous system.

54 ermophilic archaeron Sulfolobus tokodaii was cloned and expressed in E. coli.

55 se from Narcissus sp. and Galanthus spp. was cloned and expressed in Escherichia coli Biochemical ana

56 GroEL gene from Mycoplasma gallisepticum was cloned and expressed in Escherichia coli to facilitate t

57 ive GH10 endo-beta-1,4-xylanase, Xyl10E, was cloned and expressed in Escherichia coli.

58 The gene was cloned and expressed in Escherichia coli.

59 Target 5-HT receptors were cloned and expressed in the HEK293 cell line for functio

60 gulated at different moisture gradients, was cloned and expressed in tobacco.

61 Wild-type and mutant bCYP3A cDNAs were cloned and expressed in V79 cells.

62 ed potassium channel (SK) subunits have been cloned and found to preferentially form heteromeric chan

63 ormation, but not in diploids that have been cloned and frozen.

64 including two localised to the plastid, were cloned and functionally characterised.

65 Here we cloned and functionally characterized NCCbeta from the E

66 lergen Bet v 1 was the first such gene to be cloned and its product characterized.

67 This gene was cloned and over-expressed in Escherichia coli BL21(DE3).

68 The Winspit BoMYB29 CDS was cloned and overexpressed in Winspit and in the DH AG1012

69 M.HpyAXVII in the H. pylori biology, here we cloned and overexpressed the hp1369-70 fusion construct

70 r a beta-glucosidase) of D. bruxellensis was cloned and purified.

71 Its promoter gm-hir1 was cloned and revealed to strongly express a fluorescence r

72 igh throughput methods to sequence 18S rRNA, cloned and sequenced 28S rRNA and microscopically counte

73 AA adjacent to TAP2 as in domestic ducks, we cloned and sequenced genomic UAA-TAP2 fragments from all

74 We have previously cloned and sequenced the lnm gene cluster from Streptomy

75 We cloned and sequenced the promoter of the DBR2 gene from

76 We recovered mRNA from PBMCs from two cats, cloned and sequenced the variable and constant domains o

77 These viruses were also cloned and sequenced through Sanger sequencing to confir

78 The cDNA of the mature trypsin was cloned and sequenced.

79 etabolic regulation, the Leishmania GMPR was cloned and shown to be sufficient to complement the guaC

80 aspase genes were identified, the cDNAs were cloned and the recombinant enzymes were obtained after t

81 The gene was cloned and the sequence was codon optimized and expresse

82 We purified, cloned, and characterized 13 IgGs specific for several t

83 novo transcriptome assembly, we identified, cloned, and characterized 9 previously undiscovered fluo

84 he typical stacked-brick binding pattern was cloned, and deletion of the cluster was performed.

85 of natural Rhi o 2, the full-length cDNA was cloned, and expressed as recombinant (r) allergen.

86 om Escherichia coli The gene was identified, cloned, and functionally expressed in the Omp-deficient

87 uence of RV-A16 from a previous inoculum was cloned, and inoculum virus was produced using reverse ge

88 The majority of antibodies that we isolated, cloned, and sequenced belonged to the IgM isotype of the

89 IgE transcripts were amplified, cloned, and sequenced using RT-PCR.

90 lthy volunteers were primed to piperacillin, cloned, and subjected to the similar analyses.

91 These variants were cloned, and then expressed using the magnICON transient

92 ver, the long-standing question of whether a cloned animal undergoes an accelerated aging process is

93 rtunity to study the health and longevity of cloned animals compared with their cell donors.

94 The health of cloned animals generated by somatic-cell nuclear transfe

95 ing of somatic cells to enable production of cloned animals.

96 ility of early-onset age-related diseases in cloned animals.

97 Nonpathogenic TC-derived virus N13R10 (cloned as a bacterial artificial chromosome [BAC]) has a

98 gen-binding nucleotide coding sequences were cloned as a single chain into a lentiviral backbone and

99 Here, FEN1 was cloned as a suppressor of transcriptional gene silencing

100 The strain C500 has been cloned as an infectious, pathogenic bacterial artificial

101 s with "matched" heavy and light chains were cloned as IgG1, and those of high affinity for specific

102 We have now produced the first documented cloned Atlantic salmon lines.

103 egments on mature peptide part of MAF-1 were cloned, based on the primers designed according to the c

104 the evolution of this regulatory circuit, we cloned both ABA-signaling elements, SLAC1 and OST1, from

105 We have cloned both; while highly conserved in domain organizati

106 A second C5a receptor, C5L2, has also been cloned but has received much less attention because its

107 pacX was cloned by impala transposon mutagenesis.

108 g a region of interest that are subsequently cloned by recombination into a luciferase reporter vecto

109 yamine oxidase (PAO) gene was identified and cloned by screening suppression subtractive hybridisatio

110 Pigs were cloned by transfer of chromatin from swine primary fetal

111 Our group originally found and cloned cDNA for a 98-kDa type 1 transmembrane glycoprote

112 igonucleotide primers for qRT-PCR assays and cloned cDNA plasmids corresponding to the Defa paralogs.

113 a major disruption of signaling between the cloned conceptus and the endometrium, particularly the i

114 Our results support a "bottleneck" model for cloned conceptus survival during the periimplantation pe

115 that might be affected by the presence of a cloned conceptus, ultimately leading to mortality before

116 sed genes were affected by the presence of a cloned conceptus, whereas at d 34, approximately 36% and

117 that can recruit SMAD proteins, which, when cloned, could confer induction by TGFbeta.

118 alpha1)-DeltaN in neurodevelopment, we first cloned (CPE/NF-alpha1)-DeltaN transcripts from an embryo

119 Here we present the first individually cloned CRISPR-Cas9 genome wide arrayed sgRNA libraries c

120 ased by 80% in old KO mice and sequencing of cloned Dicer mRNA revealed the complete absence of the f

121 Bait-prey junctions are cloned directly from isolated genomic DNA using LAM-PCR

122 The longevity of both the donor and the cloned dog was close to the median lifespan of Afghan ho

123 vity and health of Snuppy, the world's first cloned dog, and its somatic cell donor, Tai, a male Afgh

124 ein the Bcr 3' untranslated region (UTR) was cloned downstream of a luciferase reporter showed repres

125 The mutation allowed cas9 to be cloned downstream of the strong P(thl) promoter.

126 Moreover, H3K9me2 levels were decreased in cloned embryos regardless of treating either donor cells

127 d apoptotic status of bovine preimplantation cloned embryos.

128 ing to developmental failure in somatic cell cloned embryos.

129 tes to the developmental defects observed in cloned embryos.

130 ly transcribed in guinea pig cells, (ii) the cloned enRep-M9l transcript can express a protein of the

131 rophage-tropic proteins, while the remaining cloned env genes encoded R5 T cell-tropic proteins.

132 We focus here on studies using cloned eRNAs to study their function as transcripts, rev

133 PIEZO1 is a recently cloned eukaryotic cation-selective channel that opens wi

134 In this study, we cloned, expressed and characterized all of the glycoside

135 We successfully cloned, expressed and purified this putative cysteine pr

136 Here, we cloned, expressed, and characterized Rab5a and Rab5b fro

137 Here, we cloned, expressed, and characterized the GTPase LdSar1 a

138 In this study, Stt7 kinase has been cloned, expressed, and purified in a heterologous host.

139 Similarly, surface phenotypes of individual cloned fibroblast-like cells exhibit significant variati

140 We identified and cloned four AQPs from C. lectularius, assessed tissue an

141 striking differences in protein yields when cloned from a chloramphenicol resistant vector into an i

142 IgG AHAs cloned from a single human donor exhibited restricted sp

143 k contrast to reports on Dolly (first animal cloned from adult cells) whose diagnoses of osteoarthrit

144 Luc, which expresses an env gene (C.Du151.2) cloned from an acute heterosexually infected woman and a

145 However, TCRs cloned from an effective and one of two ineffective clon

146 About 61 candidate genes were successfully cloned from Anopheles gambiae and expressed in insect ce

147 tylserotonin-O-methyltransferase (ASMT), was cloned from apple rootstock, Malus zumi.

148 lum, and although alpha-like OctRs have been cloned from Balanus improvisus (BiOctR) and Drosophila m

149 Drug-specific T cells were cloned from blood and inflamed skin, and cellular phenot

150 n this study, five alginate lyase genes were cloned from Cellulophaga algicola DSM 14237 genomic DNA,

151 le fragment complementation system for COase cloned from Chromobacterium sp.

152 antiporter-encoding genes were isolated and cloned from complementary DNA (EcNHX1-EcNHX4).

153 The MfR protein subunits Met and SRC, cloned from Daphnia pulex, were fused to the fluorophore

154 a panel of human recombinant antibodies was cloned from different B cell subpopulations, and the pre

155 Here, we survey 332 NBS-LRR genes cloned from five resistant Oryza sativa (rice) cultivars

156 274 and PDCD1LG2 cis-regulatory regions were cloned from genomic DNA and assessed in full or by mutat

157 ear transfer to generate three lines of mice cloned from iNKT nuclei.

158 By studying antibodies cloned from patients with heterologous secondary infecti

159 Fertile mice were cloned from several neurons, establishing the compatibil

160 the reactivities of more than 450 antibodies cloned from single B cells from human fetal liver, bone

161 , Vps20, Vps24, Snf7, Vps46, and Vps60) were cloned from Spodoptera frugiperda Using a viral compleme

162 A type 2 metallothionein gene, SsMT2, was cloned from Suaeda salsa, a salt- and alkali-tolerant pl

163 The HCV1406 TCR was cloned from T cells that expanded when a hepatitis C vir

164 Six TAs are cloned from tea, strawberry (Fragaria x ananassa, Fa) an

165 quence identity with a proteinase previously cloned from the American cockroach (Per a 10).

166 of a single recombinant insulin-like peptide cloned from the Aplysia CNS cDNA replicated both the enh

167 The two genes were cloned from the chromosome of Pseudomonas aeruginosa.

168 and functional profile of the Ca(V)2 channel cloned from the early-diverging animal Trichoplax adhaer

169 re found in CD247 complementary DNAs (cDNAs) cloned from the patient as well as in cDNA and genomic D

170 raniol into the secologanin pathway was also cloned from the trancriptome resource.

171 SlCAT2 was cloned from tomato flower cDNA, over-produced in Escheri

172 emical function of two members of this group cloned from V. minor (VmPiNMT) and R. serpentina (RsPiNM

173 Here, a PV was isolated and cloned from Vietnamese P. monodon specimens, designated

174 s been implicated in bystander apoptosis, we cloned full-length Envs from plasma of viremic patients

175 We cloned full-length GLP-1 receptor (GLP-1R) mRNA from a h

176 To address this systematically, we cloned full-length open reading frames of alternatively

177 We cloned Gal-1 promoter and identified the transcription f

178 ations above wild-type levels via exogenous, cloned-gene expression compensated for inefficient B pro

179 ly undocumented expression of 35% and 90% of cloned genes at 2 and 4 days post-fertilization, respect

180 To validate these in silico results, we cloned genes encoding candidate acid phosphatases from g

181 Cloned genes in the vicinity of the four QTLs were also

182 It is now possible to deliver cloned genes safely and stably to specific retinal cell

183 omerulonephritis QTL (Crgn) and positionally cloned genes underlying Crgn1 and Crgn2, which accounted

184 e known molecular function of the individual cloned genes.

185 We cloned genetically divergent env genes from the plasma o

186 igen reactivity profile and were tested with cloned GGTA1/CMAH/B4GalNT2 glycan knockout porcine cells

187 By homology search, we also identified and cloned GPCAT genes from three plant species.

188 emical validation, a full-length ZmTps21 was cloned, heterologously expressed in Escherichia coli, an

189 noxy)pyrrolidine-2-carboxylic acid (1b), for cloned homomeric kainic acid receptors subtype 1 (GluK1)

190 rve was prepared with a dilution series of a cloned human anti-ADAMTS13 autoantibody in ADAMTS13-depl

191 shown to change the activity of CHRMs using cloned human CHRMs and CHRM knockout mice but not human

192 The cloned IgG-specific AHA-variable regions were mutated fr

193 We cloned immunoglobulin G (IgG) variable domains from cryo

194 ating stable cell lines the sgRNAs have been cloned in a lentivirus backbone containing PiggyBac tran

195 ic G protein-coupled receptors (GPCRs) to be cloned in any organism.

196 Salmonella typhimurium InvA gene sequences (cloned in E. coli and after 30-cycle PCR) with SYBR((R))

197 e sufficient to repress a reporter gene when cloned in genome-integrated lentiviral reporter construc

198 ising simultaneously two donor DNA templates cloned in plasmids with different antibiotic resistant g

199 hSGLT1 gene was cloned in two different plasmids to develop parallel str

200 All 3 mutants cloned in Xenopus oocytes caused an aberrant modulation

201 liver a 152 kb herpes simplex virus 1 genome cloned in yeast into mammalian cells to produce infectio

202 Genomes can be cloned; individuals cannot.

203 The full-length cDNA was cloned into a baculovirus expression vector, and recombi

204 s that adopted a G-quadruplex structure were cloned into a luciferase dual vector and examined for th

205 omain from the collagen alpha3(IV) chain was cloned into a mammalian expression vector, pCI-neo, with

206 native flanking sequences were amplified and cloned into a pCAG-eGFP vector directed by the ubiquitou

207 y 100 nt homology arms and gRNA target sites cloned into a plasmid.

208 l receptor (TCR) alpha- and beta-chains were cloned into a retroviral vector.

209 terleukins and chemokines, were individually cloned into adeno-associated viral vectors.

210 hromosome, are synthesized in vitro and then cloned into allelic exchange vectors using standard proc

211 mplified from patient-derived serum samples, cloned into an expression vector, and used to generate v

212 mcr-3.5 was cloned into E. coli DH5alpha.

213 A full-length Cx43 cloned into mammalian expression vector pCI-neo was used

214 Two hundred nine FSPs were selected and cloned into nonhuman Great Ape Adenoviral and Modified V

215 These isoforms were subsequently cloned into recombinant adeno-associated viral (AAV) vec

216 us regions of the HABP2 promoter region were cloned into reporter constructs, and the promoter activi

217 the protease gene, cathepsin S (Rs-cps), was cloned into the binary vector pFGC5941 in the forward an

218 we used an animal model, wherein NC1-peptide cloned into the pCI-neo mammalian expression vector was

219 9 and 36 residues from the C-terminus, were cloned into the recombinant expression vector pET-28a-Pe

220 e coding sequences of FvXTH9 and FvXTH6 were cloned into the vector pYES2 and expressed in Saccharomy

221 TRPM2, the second subfamily member to be cloned, is expressed in many tissues including brain, he

222 ck garden ant (Lasius niger), identified and cloned its cognate receptor and determined its pharmacol

223 rulence factors and we confirm results using cloned knock-out parasites.

224 In HEK293 cells co-transfected with cloned Kv4.3 (or Kv4.2) and beta-subunit KChIP2, NS5806

225 204 cloned lines trapped diverse proteins, including 64 orth

226 PDE3A promoter studies were performed in a cloned luciferase construct.

227 ssays and sequence analysis of single-colony cloned M. genitalium organisms.

228 PSTVd genomes from plants inoculated with a cloned master sequence revealed naturally occurring vari

229 F) binding sites in the AaTPS1 promoter, and cloned members of both TF classes were able to activate

230 ase resistance (R) genes were identified and cloned more than two decades ago.

231 Here, we reported a newly cloned mosquito Aedes (Ae.) aegypti salivary allergen.

232 we use the quantified symptom intensities of cloned MSV isolates in differentially resistant maize ge

233 Here, we cloned multiple Env sequences from 7 HIV-2-infected pati

234 to monitor the growth of pools of previously cloned mutants and reproducibly differentiated between d

235 gsk3, tkl3, and PBANKA_082960 by genotyping cloned mutants.

236 key factors to control viral replication we cloned Mx1 cDNAs from three bat families, Pteropodidae,

237 Here we cloned naked mole-rat ASIC3 (nmrASIC3) and used a cell-s

238 Moreover, the display level of a cloned Nanobody on the surface of an individual yeast ce

239 ph1 clustered separately from all previously cloned NLRs from the Triticeae and displayed highest seq

240 We cloned nod positionally and found that it encodes CELL N

241 Analysis of cloned Ntcp from all species revealed a pronounced role

242 We cloned one of these antibodies, 3H3, from memory B cells

243 s (Rht-D, Vrn-B1, and Vrn-D1) that have been cloned or known QTLs (TaGA2ox8, APO1, TaSus1-7B, and Rht

244 sequencing applications using collections of cloned ORFs as probes.

245 M. tuberculosis DapE (MtDapE) was cloned, over-expressed and purified as an N-terminal hex

246 lic archaeon Archaeoglobus fulgidus has been cloned, over-expressed in Escherichia coli and biochemic

247 KEY MESSAGE: The vacuolar SlCAT2 was cloned, over-produced in E. coli and reconstituted in pr

248 the putative B. adolescentis 20beta-HSDH was cloned, overexpressed, and purified.

249 iminosum DNA was confirmed by sequencing the cloned PCR products, and while alignment of the ITS ampl

250 Here, we cloned PIK3CD alternative transcripts in human brain and

251 onor templates (synthesized linear dsDNA and cloned plasmid DNA constructs).

252 Jurkat cells and in vitro methylation of the cloned PLS3 promoter suppressed luciferase expression in

253 f nematode effectors in parasitic plants, we cloned predicted effectors genes from Heterodera avenae

254 roducts was assessed by Sanger sequencing of cloned products.

255 qubit at room temperature, which stores the cloned quantum states for a millisecond under ambient co

256 If several cloned R genes were available, it would be possible to p

257 ies of >55 nM were found for all other human-cloned receptor subtypes tested.

258 for the missing ARPP phosphatase were found, cloned, recombinantly expressed, and purified.

259 rim5 homologues restricted any of a panel of cloned retroviruses.

260 We cloned sabaeus CD4 and 10 candidate coreceptors.

261 A recombinant Asd(+) plasmid pCZ1 with the cloned Salmonella Choleraesuis O-antigen gene cluster wa

262 DNA of the protein was cloned, sequenced, and expressed, confirming its identit

263 Phylogenetic analysis of the cloned sequences demonstrated a high diversity of unchar

264 arative genomic hybridization, mate pair and cloned sequences, and FISH analyses, we have identified

265 recent report detailing the health status of cloned sheep concluded that the animals had aged normall

266 sure measurements in 13 aged (7-9 years old) cloned sheep, including four derived from the cell line

267 l long-term health effects in a cohort of 13 cloned sheep.

268 urally conceived sheep, and our healthy aged cloned sheep.

269 Trans-complementing the deletion with the cloned sitABCD genes confirmed that they accounted for t

270 Molecularly cloned SIVmac239 is difficult for antibodies to neutrali

271 The cloned Sr60 also can be a useful component of transgenic

272 The RON3 gene was map-based cloned starting from the ron3-1 leaf mutant and found to

273 the previously identified 32 aa peptide was cloned successfully.

274 Here we cloned, tagged and expressed 26 of the 29 SARS-CoV-2 pro

275 We cloned the entire genome of M. mycoides in yeast and use

276 Here, we cloned the FHB resistance gene Fhb7 by assembling the ge

277 We cloned the first leaf rust resistance gene Rph1 (Rph1 a)

278 We cloned the full-length EB1 cDNA for its overexpression i

279 We cloned the FZL gene based on the lesion mimic phenotype

280 We cloned the gene encoding the Yellow Fluorescence Protein

281 Therefore, we cloned the maize Tel2 and Tti1 homologs and showed that

282 We cloned the prototypic EBV-neutralizing antibody, 72A1, a

283 us for mutants defective in IT formation and cloned the responsible gene, ERN1, encoding an AP2/ERF t

284 -mediated recombineering (LLHR), we directly cloned the skt gene cluster using the Streptomyces site-

285 conserved in an invertebrate SLO2 channel we cloned the SLO2 channel fromDrosophila(dSLO2) and compar

286 We previously cloned the taqIIRM gene, purified the recombinant protei

287 e contribution to CD8(+) T-cell function, we cloned the TCR alpha and beta chain genes from one effec

288 ents to use in a RACE PCR-based approach and cloned three full-length OSC transcripts from cork (QsOS

289 and crop species, and although none has been cloned to date, transcript profiling experiments have id

290 Each data analysis job can be shared or cloned to disseminate the knowledge gained, thus propaga

291 The selected plants were cloned to reduce genetic variability within each genotyp

292 omic annotations for a recently positionally-cloned Tst gene (Thiosulfate Sulfur Transferase, synonym

293 nonetheless reversible for wild-type and sub-cloned U2OS cells, except for lasting genomic difference

294 ins critical for ESE function, MYC ESEs were cloned upstream of a green fluorescent protein (GFP) rep

295 In this method, CRSs are cloned upstream of a minimal promoter and reporter gene,

296 mportant genes in vivo, their promoters were cloned upstream of the luxCDABE operon, and luciferase e

297 the generation of infectious molecules from cloned viral sequences.

298 Sequencing of genomes of passaged and cloned viruses revealed mutations near the N terminus of

299 The cloned viruses revealed typical acute and set point vira

300 s are not yet fully understood, we generated cloned viruses with all the necessary characteristic of