ライフサイエンスコーパス: co-translational

1 of NatA, the major human NAT involved in the co-translational acetylation of proteins.

2 al membrane insertion of the tomato HMGRs is co-translational and does not involve cleavage of an N-t

3 the fundamental point of convergence between co-translational and post-translational endoplasmic-reti

4 uced by synonymous mutations cause shifts in co-translational and post-translational folding pathways

5 Glycosylation is a complex co-translational and post-translational modification (PT

6 roteins, is one of the most abundant protein co-translational and post-translational modifications.

7 a challenge for cells, which is mitigated by co-translational and sequential events.

8 ed-coils, and short linear motifs may act as co-translational assembly domains.

9 We show that co-translational assembly is governed by structural char

10 ynthesized proteins, recent work showed that co-translational assembly is prevalent in human cells.

11 ndings provide mechanistic insights into the co-translational assembly of GPCR heteromeric complexes

12 mRNA translational control, and demonstrated co-translational assembly of initiation factor complexes

13 During the co-translational assembly of protein complexes, a fully

14 t proteasome, involving ribosome pausing and co-translational assembly of Rpt1 and Rpt2.

15 2-based predictions, we accurately predicted co-translational assembly, including pair identities, at

16 cleoporins for their potential to facilitate co-translational assembly.

17 Co-translational association of mRNAs encoding subunits

18 RAB13 translation leads to a co-translational association of nascent RAB13 with the e

19 general principle allows us to predict their co-translational binding across the proteome based on se

20 acids are likely to be chaperoned during the co-translational biogenesis of membrane proteins; howeve

21 i-based in vitro translation system to allow co-translational capture of translated products by affin

22 stitution reveal that Ypl225w functions as a co-translational chaperone by forming dual interactions

23 However, the principles of co-translational chaperone interaction throughout the pr

24 dynamics of the ribosome-bound state of the co-translational chaperone trigger factor (TF).

25 gnal peptide breaks the alpha helix allowing co-translational cleavage.

26 We hypothesize that co-translational cofactor binding and targeting might be

27 Asparagine-linked glycosylation, the co-translational covalent attachment of carbohydrates to

28 CARSs also catalyze co-translational cysteine polysulfidation and are involv

29 Strikingly, co-translational decay targets encode proteins with high

30 tor (CFTR) is one ERAD substrate targeted to co-translational degradation by the E3 ligase RNF5/RMA1.

31 The co-translational degradation of apoB is controlled by fa

32 gher for soluble RNAs, but the proportion of co-translational degradation relative to the overall mRN

33 dicate that, as RNF5, RNF185 targets CFTR to co-translational degradation.

34 We here reveal the first stages of co-translational domain folding and assembly of CFTR, th

35 ture and function of the encoded proteins by co-translational effects, we sought to test whether rs68

36 Bioinformatic analysis identified reduced co-translational ER targeting of secreted and membrane-a

37 ical activity of StAR and that this post- or co-translational event accounts, in part, for the immedi

38 codons as amino acid-specifying codons is a co-translational event that enables C-terminal extension

39 st that translocation arrest with subsequent co-translational exposure to the cytosol provides an alt

40 that their primary function is to facilitate co-translational folding after synthesis of an autonomou

41 slow translational kinetics could facilitate co-translational folding and assembly of its capsid prot

42 of the free energy landscapes that underlie co-translational folding and discuss the critical coupli

43 ing represents a trade-off between promoting co-translational folding and sterically shielding the na

44 s is mediated by improving the efficiency of co-translational folding and subsequent protein stabilit

45 .Proteins fold under mechanical force during co-translational folding at the ribosome.

46 tructural elements, act as gauges of protein co-translational folding by reducing ribosome speed when

47 ple, dynamic mechanism for the modulation of co-translational folding by the ribosome.

48 ly considered to be sufficiently narrow that co-translational folding can begin only when specific se

49 tic model that calculates a protein domain's co-translational folding curve during synthesis using on

50 Our approach explains essential features of co-translational folding curves and predicts how varying

51 Unexpectedly, co-translational folding does not proceed unidirectional

52 ations affect ribosomal elongation speed and co-translational folding dynamics.

53 during biosynthesis on the ribosome(1), and co-translational folding energetics, pathways and outcom

54 e uL23 and uL24 loops is sufficient to alter co-translational folding energetics, which we highlight

55 e extent of binding result in a shift in the co-translational folding equilibrium towards the native

56 th single-molecule experiments, we delineate co-translational folding for a set of GTPase domains wit

57 To quantitatively define co-translational folding in live cells, we developed a h

58 ional elongation of the protein chain during co-translational folding in the cell, where insertion is

59 N-terminal GAU codons may facilitate correct co-translational folding in vertebrates.

60 olymerogenic mutant, Z AAT, forms a distinct co-translational folding intermediate relative to wild-t

61 Our findings demonstrate that co-translational folding intermediates drive how some pr

62 llowing nascent chain release however, these co-translational folding intermediates guide post-transl

63 s, which reveals compacted, partially-folded co-translational folding intermediates possessing molten

64 Co-translational folding is a fundamental process for th

65 Co-translational folding is assumed to simplify the conf

66 Because cellular co-translational folding is challenging to detect, its t

67 Co-translational folding is crucial to ensure the produc

68 in timescale between folding and synthesis, co-translational folding is thought to occur at equilibr

69 this model accurately predicts the course of co-translational folding measured in vivo for four diffe

70 l, Roeselova et al.(1) provide insights into co-translational folding of a multidomain protein in bac

71 by CRISPR/Cas9 gene editing, and studied the co-translational folding of an immunoglobulin-like filam

72 fold under the cradle created by TF, but the co-translational folding of larger proteins is slowed do

73 Chaperones are essential to the co-translational folding of most proteins.

74 These results suggest a pathway for the co-translational folding of repeat proteins and have imp

75 han specific codon identity-could coordinate co-translational folding of the encoded protein.

76 single-molecule experiments, we followed the co-translational folding of the G-domain, encompassing t

77 ool that simulates ribosome kinetics and the co-translational folding of the mRNA in response, I show

78 The results show that the co-translational folding of the WT pro-SP-C TM segment i

79 ypeptides fold efficiently by sequential and co-translational folding of their domains.

80 ase components) is sufficient for successful co-translational folding of two bacterial alpha-helical

81 ectroscopy to provide a basis for studies of co-translational folding on the ribosome of this immunog

82 f this interaction and the energetics of the co-translational folding process itself.

83 es, that TF does not alter significantly the co-translational folding process of a small protein G do

84 vitro for the majority of proteins relate to co-translational folding remains unclear.

85 Time-resolving structure formation during co-translational folding revealed different secondary an

86 ate the role of the exit tunnel structure in co-translational folding, and provide principles for how

87 not insert stably when ribosome-bound during co-translational folding, as they require insertion of d

88 hile a coupling between translation rate and co-translational folding, likely involving an interplay

89 During co-translational folding, the nascent polypeptide chain

90 g of the main E. coli chaperones involved in co-translational folding, Trigger Factor (TF) and DnaK c

91 r understanding key aspects of the nature of co-translational folding.

92 might allow the ribosome itself to modulate co-translational folding.

93 proteins overcome inherent challenges during co-translational folding.

94 nascent chain complexes is key to understand co-translational folding.

95 rocesses, ranging from muscle contraction to co-translational folding.

96 cell-free approach to develop tools to probe co-translational folding.

97 In particular, they suggest that major co-translational functions of bacterial tunnels were ext

98 eling dramatically enhances the detection of co-translational glycopeptides with low abundance when e

99 Further analyses revealed that among co-translational glycoproteins, those related to DNA bin

100 We also explore how the emergence of co-translational glycosylation and the expansion of the

101 form of the OST is primarily responsible for co-translational glycosylation of the nascent polypeptid

102 tion (IVT) systems, we found that inhibiting co-translational iMet processing does not impact ribosom

103 h would support the conditions necessary for co-translational import.

104 inct classes can be engineered to direct the co-translational incorporation of diverse alpha hydroxy

105 train of Escherichia coli for site-specific, co-translational incorporation of phosphoserine into pro

106 que family of proteins, characterized by the co-translational incorporation of selenium as selenocyst

107 SelB is an elongation factor needed for the co-translational incorporation of selenocysteine.

108 Co-translational insertion and folding is thus spontaneo

109 o reach their sites by diffusion after their co-translational insertion in the rough endoplasmic reti

110 itochondrial genome are hydrophobic and need co-translational insertion into a lipid bilayer, respons

111 ial translocon and its efficient function in co-translational insertion into and translocation across

112 l-tRNA synthetase and tRNA pairs that enable co-translational insertion of a phospho-amino acids, has

113 he direct simulation of trajectories for the co-translational insertion of arbitrary polypeptide sequ

114 thereby co-ordinating pigment delivery, the co-translational insertion of LH polypeptides and their

115 c mismatch, which reduces the expression and co-translational insertion of membrane proteins into syn

116 ochondrial ribosomes and plays a role in the co-translational insertion of mitochondria-synthesized p

117 of the ribosome with the insertase promotes co-translational insertion of nascent chains.

118 oM methylase and the pyl operon required for co-translational insertion of pyrrolysine into the activ

119 lts establish that SBP2 is essential for the co-translational insertion of Sec into selenoproteins.

120 Co-translational insertion of selenocysteine (Sec) into

121 Selenoprotein biosynthesis relies on the co-translational insertion of selenocysteine in response

122 Membrane enrichment is caused by co-translational insertion of signal peptides recognized

123 er synthesis of TMDs 1 and 2, and that after co-translational insertion of the remaining TMDs, redund

124 ognition particle (SRP) targets proteins for co-translational insertion through or into the endoplasm

125 e C-terminal region show specific defects in co-translational insertion, suggesting that the close co

126 t simulation for the mechanistic analysis of co-translational integration and for the engineering of

127 er supported by the fact that inhibiting the co-translational interaction of hdeltaOR-Cys(27) precurs

128 tifs and identify several previously unknown co-translational interactions.

129 of apoB support the intrinsic capability of co-translational lipid recruitment.

130 cytosol and targeted to the proteasomes in a co-translational manner.

131 n to form structure in a highly coordinated, co-translational manner.

132 Here, we elucidate the co-translational mechanism responsible for the expressio

133 As a test for a co-translational mechanism, a chimera of GFP that contai

134 is are carried out by bespoke chaperones but co-translational mechanisms tailored to eEF1A folding re

135 everal recent discoveries highlight multiple co-translational mechanisms that modulate mRNA translati

136 This co-translational membrane insertion and processing requi

137 During co-translational membrane insertion of membrane proteins

138 ribosome nascent chain complex, allowing for co-translational membrane insertion, whereas loop C1 may

139 verages a recently demonstrated link between co-translational membrane integration efficiency and pro

140 e dual signal chimera provides support for a co-translational mitochondrial import pathway.

141 ther, these data suggest a mechanism for the co-translational mode of YidC-mediated membrane protein

142 t some limited topological malleability, the co-translational model likely dominates under normal cir

143 A second, co-translational model, posits that the protein inserts

144 N-myristoylation is a co-translational modification occurring for members of t

145 The co-translational modification of N-terminal acetylation

146 Amino-terminal acetylation is a critical co-translational modification of the newly synthesized p

147 pansion of the functional repertoire of this co-translational modification.

148 Here, our in vitro data reveals evidence of co-translational modulation in 5-HT(2A)R and mGluR2 mRNA

149 ts the complexity of sequence motifs driving co-translational mRNA decay in eukaryotes, and presents

150 s been shown to affect codon usage-dependent co-translational mRNA decay in yeast.

151 the full diversity of sequences that trigger co-translational mRNA decay is poorly understood.

152 sting that the fragments may be generated by co-translational mRNA decay.

153 Co-translational mRNA degradation is a widespread proces

154 eat effects by inducing ribosome pausing and co-translational mRNA turnover.

155 C, orients NMT2 on the ribosomal surface for co-translational myristoylation of nascent chains.

156 ese findings are the first to establish that co-translational N-glycosylation of human GGT is require

157 we show that loss of DC2 causes a defect in co-translational N-glycosylation of proteins that mimics

158 ligosaccharyltransferase complex involved in co-translational N-glycosylation.

159 he protein translocation channel to catalyze co-translational N-linked glycosylation of proteins in t

160 ately 177 kDa ALK polypeptide core undergoes co-translational N-linked glycosylation, emerging in its

161 series of covalent modifications, including co-translational N-myristoylation at Gly(2), as well as

162 he nascent protein exit tunnel, accelerating co-translational N-terminal initiator methionine (iMet)

163 More than 180 co-translational O-GlcNAcylated proteins were site-speci

164 ematic and site-specific analysis of protein co-translational O-GlcNAcylation can advance our underst

165 ive method was developed to identify protein co-translational O-GlcNAcylation, which is very useful t

166 y and site-specifically characterize protein co-translational O-GlcNAcylation.

167 are very low abundant and the abundances of co-translational ones are even much lower.

168 ese proteins cannot follow the SRP-dependent co-translational pathway that typifies most integral mem

169 transmembrane domain that cannot access the co-translational pathway.

170 l inner membrane proteins are assembled by a co-translational process directed by SRP/FtsY, the SecYE

171 his meant that an important dimension of the co-translational process remained unstudied.

172 n the ER, (b) heterodimer formation is not a co-translational process, and (c) heme insertion is a de

173 states that are far removed from the natural co-translational process.

174 thesis, potentially influencing simultaneous co-translational processes such as folding and chemical

175 the ribosome to carefully regulate disparate co-translational processes that determine the fate of na

176 cleavage and N-glycosylation of proteins are co-translational processes, but little is known about th

177 pausing of ribosomes can affect a variety of co-translational processes, including protein targeting

178 thermodynamically regulate folding and other co-translational processes.

179 y selection indicate their potential role in co-translational processes.

180 vidence that PEXEL position is conserved for co-translational processing and export.

181 ex (NAC) orchestrates the binding of several co-translational processing factors on ribosomes, its ro

182 hionine aminopeptidase (MetAP) catalyzes the co-translational processing of initiator methionine from

183 e have assayed the 24-kD fl2 alpha-zein in a co-translational processing system in vitro.

184 zed upon heat acclimation to sustain correct co-translational protein complex assembly.

185 of stochastic compartmentalization and that co-translational protein delivery is the main contributo

186 report that sustained mRNA localization and co-translational protein delivery leads to a heterogeneo

187 low translation elongation, likely impacting co-translational protein folding and allowing improved i

188 quences revealed that rare codons can impact co-translational protein folding and that positions of s

189 ral aspects of protein biogenesis, including co-translational protein folding and translational fidel

190 Detailed structural studies of co-translational protein folding are now beginning to em

191 Co-translational protein folding is an essential process

192 mic nascent chain associations to coordinate co-translational protein folding, facilitate accelerated

193 f cellular protein homeostasis by regulating co-translational protein folding, localization, and matu

194 expression levels, ribosome localization and co-translational protein folding.

195 final protein structure and thus possibly to co-translational protein folding.

196 ical basis of the distinct thermodynamics of co-translational protein folding.

197 role in regulating translation kinetics and co-translational protein folding.

198 sage regulates protein function by affecting co-translational protein folding.

199 hanisms that mediate efficient mitochondrial co-translational protein import.

200 was associated with specific changes of the co-translational protein interaction network, including

201 -alpha-acetylation is one of the most common co-translational protein modifications in humans and is

202 ), a ribonucleoprotein (RNP) responsible for co-translational protein secretion that contains a non-c

203 recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellu

204 tors and suggest that the basic mechanism of co-translational protein targeting is conserved between

205 The SRP is a central component of the co-translational protein targeting machinery that binds

206 During co-translational protein targeting, the signal recogniti

207 ty of the protein distribution by inhibiting co-translational protein targeting.

208 lance between the efficiency and fidelity of co-translational protein targeting.

209 nfolded Protein Response is coupled with the co-translational protein translocation pathway to mainta

210 Sec61 is the key component of the mammalian co-translational protein translocation system and has be

211 access to bulk lipid, we propose a model for co-translational protein translocation.

212 elease the truncated protein product in this co-translational quality control pathway.

213 ger RNAs that lack stop codons is one of the co-translational quality control pathways.

214 Enhancing co-translational quality control prevents C-I30 C-termin

215 modulate translation elongation and impacts co-translational quality control to minimize production

216 es and inserted into the inner membrane in a co-translational reaction facilitated by the Oxa1 insert

217 Here, we show that the co-translational recognition of Rpl3 and Rpl4 by their r

218 Studies in vivo show that co-translational regulation of the viral coat and replic

219 Here, we show that co-translational ribosome pairing allows their nascent c

220 evailing view of N-terminal acetylation as a co-translational ribosome-associated process and suggest

221 At the pachytene stage, when other co-translational RNA surveillance pathways are sequester

222 mic domains requiring the dual action of the co-translational Sec and post-translational Tat pathways

223 enous opal suppression pathway competes with co-translational Sec insertion.

224 s in selenoprotein mRNAs and facilitation of co-translational Sec insertion.

225 Co-translational secondary folding of nascent chains ins

226 Furthermore, rerouting of the scFab to the co-translational signal recognition particle pathway com

227 We here detail the mechanism by which co-translational signal-peptide cleavage is prevented.

228 wo distinct N-glycosylation sites: a typical co-translational site and a consensus site approximately

229 Mutations that ablate the co-translational site concomitantly reduce glycosylation

230 of the KCNE1 post-translational site into a co-translational site restored both monoglycosylation an

231 he glycosylation sites on all glycoproteins, co-translational sites have different local structures a

232 he Ssa family of cytosolic Hsp70 and not the co-translational Ssb homolog, consistent with the lack o

233 These early co-translational steps are crucial for overall folding a

234 esting that Z's misfolding is initiated from co-translational structure.

235 embranes is one of the central themes to the co-translational targeting and import of proteins.

236 an in vitro assay that faithfully mimics the co-translational targeting and translocation of the amin

237 nthesized triglyceride (TG) are critical for co-translational targeting of apolipoprotein B (apoB100)

238 (SRP) and its receptor (SR) function in the co-translational targeting of nascent protein-ribosome c

239 recognition particle (SRP) pathway mediates co-translational targeting of nascent proteins to membra

240 ribonucleoprotein particle that mediates the co-translational targeting of newly synthesized proteins

241 l recognition particle (SRP) is required for co-translational targeting of polypeptides to the endopl

242 ) and the SRP receptor (SR) that control the co-translational targeting of proteins to cellular membr

243 ntial component of the SRP that mediates the co-translational targeting of proteins to the correct ce

244 recognition particle (SRP) that functions in co-translational targeting of proteins to the membrane.

245 GTPases Ffh and FtsY play a central role in co-translational targeting of proteins, assembling in a

246 The first step in the co-translational targeting of secretory proteins to the

247 in from the precursor did not interfere with co-translational targeting of the nascent chain to the S

248 . coli components to function in a bona fide co-translational targeting pathway remains unclear.

249 e domain, known as a signal anchor (SA), for co-translational targeting to the endoplasmic reticulum

250 In co-translational targeting, interactions among these pro

251 ase, an SP that is recognized by the SRP for co-translational targeting, it is found that substrate b

252 ligosaccharyl transferase (OT) catalyzes the co-translational transfer of a dolichol-linked tetradeca

253 bosome-rich ER membranes expanded, promoting co-translational translocation and enhanced biogenesis o

254 Ribosome stalling during co-translational translocation at the ER causes transloc

255 y to demonstrate that Sec63p is required for co-translational translocation in vitro and specifically

256 e cytoplasm, rather than allowing its normal co-translational translocation into the endoplasmic reti

257 sttranslational direct chloroplast import or co-translational translocation into the ER prior to chlo

258 demonstrate that in vitro reconstitution of co-translational translocation is greatly enhanced using

259 d that this is due to its ability to inhibit co-translational translocation of CD4 into the lumen of

260 hat associates with ribosomes to promote the co-translational translocation of proteins across biolog

261 The co-translational translocation of proteins into the endo

262 During the co-translational translocation of secretory and membrane

263 ted splitting of ribosomes that stall during co-translational translocation of secretory proteins int

264 These findings support the hypothesis that a co-translational translocation pathway exists for import

265 This co-translational translocation pathway is initiated when

266 ike cytosolic mRNAs, secretome mRNAs undergo co-translational translocation, and thus require precise

267 associates with the translating ribosome in co-translational translocation, and with the SecA ATPase

268 In co-translational translocation, the ribosome funnel and

269 doplasmic reticulum membrane is important to co-translational translocation.

270 The magnitude of co-translational ubiquitination and subsequent degradati

271 described of these degradative processes is co-translational ubiquitinylation and proteasomal degrad